376 but different for the human-baited tent, with a majority (41%) of An. by plastic containers and small pools along the way. The main specie vagus. Eight of the nine morphological species represented in this study collected was Ae. aegypti followed by Culex quinquefasciatus. Anopheles were captured on each of the three hosts, suggesting a plasticity in mosquitoes were not found in the highway area. Tire repair shops were host attraction behavior. Multiple host feeding and flexibility in feeding the places with the highest number of infected tires; we also found behavior could have important implications for malaria control. abandoned tires infected with mosquito larva. 1250 1252 ASPECTS OF ECOLOGY OF POTENTIAL RIFT VALLEY FEVER A COMPARATIVE EVALUATION OF SIX DIFFERENT MALARIA VIRUS MOSQUITO VECTORS, KHARTOUM STATE, SUDAN VECTOR COLLECTION METHODS IN LOW-LYING MALARIA ENDEMIC REGIONS OF WESTERN KENYA Deena M. Abdelgadir Federal Ministry of Health Sudan, Khartoum, Sudan Nabie Bayoh1, George Olang1, Nico Govella2, Gerry Killeen2, John Gimnig3 Rift valley fever epidemics are disruptive and expensive to local and 1Kenya Medical Research Institute, Kisumu, Kenya, 2Ifakara Health regional economies. After a devastating outbreak of Rift Valley Fever Institute, Dar es Salaam, United Republic of Tanzania, 3Centers for Disease in khartoum state, Sudan 2007; ecological baseline surveys were Control and Prevention, Atlanta, GA, United States conducted in Khartoum State, Sudan, during the rainy season (end of July to the beginning of September) 2008 in order to identify mosquito Outdoor biting and other forms of behavioral adaptation by malaria species present and evaluate their emergence and survivorship. Larval vectors to domestic insecticide-based control measures may compromise identification of species of Culicine and Anopheline mosquitoes present the sensitivity of conventional sampling tools operating indoors such as in Khartoum State taken from five study sites represents Khartoum state light traps or indoor resting catches, thus preventing effective surveillance indicated that Anopheles arabiensis is the only species of the Anopheline and management of vector populations. We evaluated six different vector mosquitoes found. Three species of culicine mosquitoes were found: collection methods to optimize a protocol for operational sampling of Culex quinquefasciatus, Cx univittatus and Cx arbeeni, Species of Aedes malaria vectors robust to variations in vector behavior, notably variations were found in irrigated schemes at one study site and was absent from associated with the presence of important malaria control methods. Over the other four study sites, these species were Ae. vittatus and Ae. vexans, 30 days, we replicated a Latin square design 10 times at sites in 4 districts whose presence was recorded after the onset of the rainy season.the in western Kenya: Kisumu, Bondo, Nyando and Rachuonyo. Each site same breeding site was first occupied by Ae.vittatus then Ae. vexans, consisted of 3 locally representative houses through which the six different with an interval of habitat drying. Daily emergent adults Culicine and sets of trapping methods were rotated every 3 nights in a random order Anopheline mosquitoes present were taken from randomly selected of three possible arrangements: 1) Indoor human landing catch (HLC) and breeding sites in the five study sites, population measurements were outdoor HLC, 2) CDC Light trap placed beside an occupied insecticide- performed. The absolute number of emergent adults was obtained by treated net indoors combined with Ifakara tent traps outdoors, and 3) collecting mosquitoes under net-traps covering the breeding sites. Records Window traps to catch exiting mosquitoes combined with both pot and were taken each day for seven constitutive days, synchronized emergence box formats of resting traps placed both indoors and outdoors. At each of males and females was observed at all the study sites, showing an site, a fourth house was selected for pyrethrum spray catch (PSC). The overall marked predominance of females in emergence trap catches. Adult top collection methods with their corresponding number of Anopheles survival rate was the most important factor determining the stability of the per collection effort were PSC (10.5), HLC indoor (3.0), Light trap (3.0) population and total egg production. Females that become infected when HLC Outdoor (2.8) and Ifakara tent traps (2.7). Resting Boxes and Pots taking a blood meal must survive throughout the incubation period of the positioned both indoors and outdoors caught less than 1 Anopheles per pathogen. Under controlled laboratory environment, effect of food types collection effort. HLC outdoor collected the highest amount of Culex at (sucrose 10%, sucrose 10% and blood diet) on longevity of adult female 77.4 per collection effort. Irrespective of the intensity or type of insecticide mosquitoes was conducted, sugar-fed and blood-fed mosquitoes exhibited based vector control method in place and of biting behavior of the local very high percentage of surviving rates beyond the 15 days (incubation malaria vectors, we conclude that pyrethrum spray catch is the most period for RVFV),However these have varied among the five study areas. sensitive method for vector collection in low lying malaria endemic regions Also results indicated prolonged survival of sugar-fed female mosquitoes of western Kenya. more than blood and sugar fed females, this served to increase survivorship of females until they find the appropriate host. 1253 1251 HETEROGENEOUS FEEDING PATTERNS OF AEDES AEGYPTI IN HOUSEHOLDS IN IQUITOS, PERU MAIN MOSQUITO BREEDING SITES FOR AEDES AEGYPTI IN 1 2 1 THE PAN-AMERICAN HIGHWAY: CUCUTA-PAMPLONA AREA Kelly Liebman , Helvio Astete , Steven T. Stoddard , Moises 3 4 5 1 (NORTE DE SANTANDER - COLOMBIA) IN 2010 Sihuincha , Eric Halsey , Tadeusz J. Kochel , Amy Morrison , Thomas W. Scott1 1 2 Berlin L. Londono-Renteria , Flaminio Londono , Jenny Carolina 1University of California, Davis, Davis, CA, United States, 2Navy Medical 3 1 Cardenas , Lucio Daniel Cardenas Research Unit-6, Iquitos, Peru, 3Hospital Apoyo Iquitos, Iquitos, Peru, 4Navy 1Universidad de Pamplona, Pamplona, Colombia, 2Politecnico Jaime Isaza Medical Research Unit-6, Lima, Peru, 5Navy Medical Research Center, Silver Cadavid, Bello, Colombia, 3Hospital Los Patios-Norte de Santander, Los Spring, MD, United States Patios, Colombia Heterogeneous biting by female mosquitoes can significantly alter Aedes aegypti is the principal dengue vector in Colombia where dengue transmission of mosquito-borne pathogens. Previous studies show Aedes transmission is limited by the presence of the vector; unfortunately in this aegypti, the primary vector of dengue viruses (DENVs), more frequently country, the presence of Ae. aegypti has been documented up to 2200 bite individuals with higher body mass index (BMI). Because BMI increases m.a.s.l. Norte de Santander is the second most endemic area for dengue with age, we expect positive linear relationship with age and biting. in the country. Previous studies have associated travel and transport as Studies show, however, that young adults receive more bites than older key factors in the spread of diseases and vectors. With this pilot study, we adults. Factors such as sex, mosquito exposure time and previous DENV investigated the main breeding sites and mosquito larva species on the infection should, therefore, be used to analyze heterogeneous feeding highway from Cucuta (325 m.a.s.l) to Pamplona (2342 m.a.s.l) in 75km patterns. Between October 2009 and November 2010, 2,035 interviews distance. We found that tires where the main breeding site followed with 280 participants were conducted in 19 households in Iquitos, Peru. www.astmh.org 377 Interviews focused on anthropomorphic characteristics and time spent in 1255 houses. In the week following interviews adult mosquitoes were collected twice daily, yielding 1,878 engorged and partially engorged mosquitoes. A COMPUTER SYSTEM FOR FORECASTING WEST NILE VIRUS Engorged abdomens were excised and participant DNA was obtained by RISK USING EARTH OBSERVATION DATA cheek swab. All DNA was extracted using Qiagen extraction columns. 1 1 1 Human DNA was amplified at 10 microsatellite loci, and allelic profiles Michael C. Wimberly , Ting-Wu Chuang , Aashis Lamsal , 1 1 2 identified using capillary electrophoresis. A computer program matched Alemayehu Midekisa , Geoffrey M. Henebry , Gabriel Senay , 1 1 participant profiles to mosquito blood meals. To date, 99 of 115 identified Michael B. Hildreth , Yi Liu blood meal profiles have been matched to participants. 29 young adults 1South Dakota State University, Brookings, SD, United States, 2USGS Earth (ages 15 - 35) received 50 bites (1.72 bites/person). 14 children (<15) and Resources Observation and Science Center, Sioux Falls, SD, United States 23 older adults (>35) received 154 and 34 bites, respectively (1.07 and Although there have been many calls to expand the use of earth 1.48 bites/person). In one household of 12 residents ranging in age from observation technologies in the health sciences, there are few examples 5 to 70 years with BMI of 13 to 32 kg/m2, 2 young adults ages 27 and 31 of operational systems with demonstrated impacts on public health. Our with BMI’s of 23 and 21.2 contributed to 46% of the 26 identified blood research objective was to bridge the gap between remote sensing and meals, consistent with the idea that young adults are bitten most often,