DOCSLIB.ORG

This Thesis Has Been Submitted in Fulfilment of the Requirements for a Postgraduate Degree (E.G

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
This Thesis Has Been Submitted in Fulfilment of the Requirements for a Postgraduate Degree (E.G This thesis has been submitted in fulfilment of the requirements for a postgraduate degree (e.g. PhD, MPhil, DClinPsychol) at the University of Edinburgh. Please note the following terms and conditions of use: This work is protected by copyright and other intellectual property rights, which are retained by the thesis author, unless otherwise stated. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This thesis cannot be reproduced or quoted extensively from without first obtaining permission in writing from the author. The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the author. When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given. Lost Pigs and Broken Genes: The search for causes of embryonic loss in the pig and the assembly of a more contiguous reference genome Amanda Warr A thesis submitted for the degree of Doctor of Philosophy at the University of Edinburgh 2019 Division of Genetics and Genomics, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh Declaration I declare that the work contained in this thesis has been carried out, and the thesis composed, by the candidate Amanda Warr. Contributions by other individuals have been indicated throughout the thesis. These include contributions from other authors and influences of peer reviewers on manuscripts as part of the first chapter, the second chapter, and the fifth chapter. Parts of the wet lab methods including DNA extractions and Illumina sequencing were carried out by, or assistance was provided by, other researchers and this too has been indicated throughout the thesis. This work has not been submitted for any other degree or professional qualifications. All sources of information have been acknowledged. Amanda Warr 2019 I Abstract The pig is an economically important species, with pork being the most widely consumed meat in the world. Genomic technologies have the potential to improve reproduction, health and efficiency in the pig industry. Additionally, pigs are more similar to humans than species commonly used as medical models and improved genomic resources for the pig may facilitate its use in medical modelling. The cost of DNA sequencing has greatly decreased in recent years, allowing more researchers to incorporate next generation sequencing into their projects. Many bioinformatic tools are designed to accept a reference genome as a truth against which individuals are compared, however most of the available reference genome sequences are low-quality drafts. It is important to understand the limitations of available reference genomes in order to make full use of the sequencing technologies available. Chapter 2 assesses the quality of the published pig draft reference genome sequence, Sscrofa10.2, using short-read sequencing data from the individual from which the genome was assembled. By identifying regions where the reads disagree with the assembly, regions of low-confidence are identified and a filter is produced to reduce the impact of these regions on genomic analyses. Chapter 3 makes use of exome sequencing data to identify variants that are predicted to truncate proteins in 96 pigs, through application of filters, including the filter designed in Chapter 2. This is reduced to a short list of variants that are likely to have an impact on phenotype, specifically variants that may be associated with reproductive phenotypes and embryonic lethality. Additionally, imputation from the 96 pig III exomes to a larger set of 446 pigs each genotyped for ~60,000 single nucleotide polymorphisms (SNPs) with the PorcineSNP60 BeadChip (Illumina) is carried out, and variants are investigated for association between two reproductive phenotypes and the imputed exome variants using a genome-wide association study (GWAS) and a number of candidate genes are identified. Chapter 4 uses an alternate method of identifying phenotype altering variants by using whole genome sequencing of a trio of individuals (sire, dam and affected individual) to search for a genetic cause of foetal mummification in pigs. Finally, chapter 5 focuses on improving the available resources for the pig by reassembling the pig genome using the latest long- read sequencing technologies, producing a much improved assembly, Sscrofa11.1. The assembly is one of the most contiguous reference genomes currently available with a contig N50 of 48.2Mb, only 103 gaps remaining (less the Y chromosome) and two closed chromosomes. This project improves the available genomic resources for the pig, and identifies several putative causal variants and candidate genes underlying important traits in a commercial population. IV Lay Summary Pork is the most widely consumed meat in the world, and it is important that we are able to produce enough pork to meet demand. In order to improve pork production, it would be beneficial to know exactly why one pig performs better or worse than others. Every living thing is made up of cells, and in those cells are a set of instructions, or “DNA”, that determine the traits of individuals along with environmental influences. We call the full set of DNA in the cell a “genome”, and the sections that specifically instruct on how to make proteins are called the “genes”. Using equipment called DNA sequencers, we can read the instructions. The DNA is written in sequences of “bases” (A, G, T and C). Most DNA sequencers can only read a few hundred bases at a time. A pig’s genome has roughly 2.6 billion bases. If we piece together sequences from the genome we can make a “reference genome”, a sequence that represents the order in which the bases are found in the complete set of DNA in an individual. Using this we can take the short sequences that DNA sequencers produce and compare them to the reference to start to understand what is different between individuals, and how those differences, or “variants”, affect the traits of the individual. For the pig, there is already a reference genome, however, in the second chapter I describe methods I used to identify parts of this reference that are badly assembled. The genome has many mistakes in it and thousands of gaps. If we compare sequences to a reference that doesn’t accurately represent any pig this can confuse analyses and make data harder to interpret. In chapter 3, I use sequences from the genes of 96 pigs and by comparing them to the V reference I try to identify variants that cause the protein to be cut short, truncating the protein potentially makes it unable to function. I use the badly assembled regions from the second chapter to exclude some inaccurate variants and identify several variants that might truncate proteins that are important for pork production. I also try to relate the presence of variants to differences in the breeding success of the pigs. In chapter 4, I look at sequences from a piglet that died during pregnancy, and both of its parents, to see if the piglet inherited any variants from its parents that may have caused its death. Each individual has two copies of a gene, one copy inherited from each parent, so even if both parents are healthy, they might each have one copy of a damaging variant, and both pass down this copy to the piglet. In chapter 2 and 3, badly assembled regions of the reference genome made accurate analysis difficult. In chapter 5, I assemble a new reference genome using a new kind of DNA sequencer that can read tens of thousands of bases at a time instead of only a few hundred. These longer sequences make it easier to find overlapping sequences and assemble the genome with fewer mistakes and fewer gaps. This new reference genome is much more accurate than the previous one and is now available for other researchers to use, and should make genome analysis in the pig more accurate and useful. This work provides other scientists with better resources to work with, and also identifies genetic variants that might cause smaller litter sizes in pigs that following further validation can be avoided to improve pork production. VI Acknowledgements I would like to thank my primary supervisor Mick Watson for his advice, support and encouragement throughout the project. I had no experience of bioinformatics and limited programming experience when I started this project, and Mick’s guidance has helped me to develop these skills. I would also like to thank the Watson group, particularly Christelle Robert who played a big part in introducing me to exome sequencing at the beginning of the project and whose data was used in chapter 3. I’d like to thank my second supervisor, Alan Archibald, who gave me the opportunity to reassemble the pig genome, this was a challenging part of the project, but also very rewarding and Alan’s enthusiasm for producing a high quality reference assembly is contagious. My final supervisor, David Hume, was also very supportive and always full of ideas and advice. All three of my supervisors have shown great faith in me, and have helped to build my confidence in my abilities, for which I am extremely grateful. My project was part funded by Genus PIC, and I’d like to thank Joseph Deeb, Matthew Campbell, Steve Rounsley and Alan Mileham for their support. Particular thanks to Joseph and Matthew, who not only advised me on science, but also arranged accommodation for me and drove me around for ~3 months while I was in the States. Several parts of this project involved wet lab work, in which I had some experience but was not confident. I’d like to thank everyone who welcomed the poor, lost bioinformatician into their lab and were always happy to answer VII silly questions and lend me equipment.
Load more

Recommended publications
  • Relationality and Masculinity in Superhero Narratives Kevin Lee Chiat Bachelor of Arts (Communication Studies) with Second Class Honours
    i Being a Superhero is Amazing, Everyone Should Try It: Relationality and Masculinity in Superhero Narratives Kevin Lee Chiat Bachelor of Arts (Communication Studies) with Second Class Honours This thesis is presented for the degree of Doctor of Philosophy of The University of Western Australia School of Humanities 2021 ii THESIS DECLARATION I, Kevin Chiat, certify that: This thesis has been substantially accomplished during enrolment in this degree. This thesis does not contain material which has been submitted for the award of any other degree or diploma in my name, in any university or other tertiary institution. In the future, no part of this thesis will be used in a submission in my name, for any other degree or diploma in any university or other tertiary institution without the prior approval of The University of Western Australia and where applicable, any partner institution responsible for the joint-award of this degree. This thesis does not contain any material previously published or written by another person, except where due reference has been made in the text. This thesis does not violate or infringe any copyright, trademark, patent, or other rights whatsoever of any person. This thesis does not contain work that I have published, nor work under review for publication. Signature Date: 17/12/2020 ii iii ABSTRACT Since the development of the superhero genre in the late 1930s it has been a contentious area of cultural discourse, particularly concerning its depictions of gender politics. A major critique of the genre is that it simply represents an adolescent male power fantasy; and presents a world view that valorises masculinist individualism.
    [Show full text]
  • Cohen, Comic Relief: Humor in Contemporary American Literature
    Studies in English, New Series Volume 4 Article 39 1983 Cohen, Comic Relief: Humor in Contemporary American Literature Charles Sanders University of Illinois, Urbana-Champagne Follow this and additional works at: https://egrove.olemiss.edu/studies_eng_new Part of the American Literature Commons, and the English Language and Literature Commons Recommended Citation Sanders, Charles (1983) "Cohen, Comic Relief: Humor in Contemporary American Literature," Studies in English, New Series: Vol. 4 , Article 39. Available at: https://egrove.olemiss.edu/studies_eng_new/vol4/iss1/39 This Article is brought to you for free and open access by the Studies in English at eGrove. It has been accepted for inclusion in Studies in English, New Series by an authorized editor of eGrove. For more information, please contact [email protected]. Sanders: Cohen, Comic Relief: Humor in Contemporary American Literature SARAH BLACHER COHEN, ED. COMIC RELIEF: HUMOR IN CONTEMPORARY AMERICAN LITERATURE. URBANA, CHICAGO, AND LONDON: THE UNIVER­ SITY OF ILLINOIS PRESS, 1978. 339 pp. $15.00. The year was 1983. Praisers of the literary imagination who believed that their praises should reflect some impassioned bit of the imaginative—those artist-critic-scholar-teacher out-of-sorts like Guy Davenport, or Richards Gilman and Howard, or George Steiner, or the brothers Fussell, for whom “excellence...is ever radical”—all these had been interned upon the new Sum-thin-Else Star. (To a neighbor­ ing star, rumor has it, must eventually come Sanford Pinsker, Earl Rovit, Max F. Schulz, and Philip Stevick, especially if they insist on writing with a brio that places them in brilliant relief to the twelve others with whom they have presently, unfortunately, been asso­ ciated.) A few remaining disciples of letters and the fine arts were now relocated in the High Aesthetic Education Camp of the One Galactic University Sandbox, Inc.
    [Show full text]
  • “The Grin of the Skull Beneath the Skin:” Reassessing the Power of Comic Characters in Gothic Literature
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Dissertations, Theses, and Student Research: Department of English English, Department of 12-2011 “The grin of the skull beneath the skin:” Reassessing the Power of Comic Characters in Gothic Literature Amanda D. Drake University of Nebraska-Lincoln Follow this and additional works at: https://digitalcommons.unl.edu/englishdiss Part of the Literature in English, British Isles Commons Drake, Amanda D., "“The grin of the skull beneath the skin:” Reassessing the Power of Comic Characters in Gothic Literature" (2011). Dissertations, Theses, and Student Research: Department of English. 57. https://digitalcommons.unl.edu/englishdiss/57 This Article is brought to you for free and open access by the English, Department of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Dissertations, Theses, and Student Research: Department of English by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. “The grin of the skull beneath the skin:” Reassessing the Power of Comic Characters in Gothic Literature by Amanda D. Drake A Dissertation Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Doctor of Philosophy English Nineteenth-Century Studies Under the Supervision of Professor Stephen Behrendt Lincoln, Nebraska December, 2011 “The grin of the skull beneath the skin:” 1 Reassessing the Power of Comic Characters in Gothic Literature Amanda D. Drake, Ph.D. University of Nebraska, 2011 Advisor: Dr. Stephen Behrendt Neither representative of aesthetic flaws or mere comic relief, comic characters within Gothic narratives challenge and redefine the genre in ways that open up, rather than confuse, critical avenues.
    [Show full text]
  • Structural Variant Calling by Assembly in Whole Human Genomes: Applications in Hypoplastic Left Heart Syndrome by Matthew Kendzi
    STRUCTURAL VARIANT CALLING BY ASSEMBLY IN WHOLE HUMAN GENOMES: APPLICATIONS IN HYPOPLASTIC LEFT HEART SYNDROME BY MATTHEW KENDZIOR THESIS Submitted in partial FulFillment oF tHe requirements for the degree of Master of Science in BioinFormatics witH a concentration in Crop Sciences in the Graduate College of the University oF Illinois at Urbana-Champaign, 2019 Urbana, Illinois Master’s Committee: ProFessor MattHew Hudson, CHair ResearcH Assistant ProFessor Liudmila Mainzer ProFessor SaurabH SinHa ABSTRACT Variant discovery in medical researcH typically involves alignment oF sHort sequencing reads to the human reference genome. SNPs and small indels (variants less than 50 nucleotides) are the most common types oF variants detected From alignments. Structural variation can be more diFFicult to detect From short-read alignments, and thus many software applications aimed at detecting structural variants From short read alignments have been developed. However, these almost all detect the presence of variation in a sample using expected mate-pair distances From read data, making them unable to determine the precise sequence of the variant genome at the speciFied locus. Also, reads from a structural variant allele migHt not even map to the reference, and will thus be lost during variant discovery From read alignment. A variant calling by assembly approacH was used witH tHe soFtware Cortex-var for variant discovery in Hypoplastic Left Heart Syndrome (HLHS). THis method circumvents many of the limitations oF variants called From a reFerence alignment: unmapped reads will be included in a sample’s assembly, and variants up to thousands of nucleotides can be detected, with the Full sample variant allele sequence predicted.
    [Show full text]
  • ELEMENTS of FICTION – NARRATOR / NARRATIVE VOICE Fundamental Literary Terms That Indentify Components of Narratives “Fiction
    Dr. Hallett ELEMENTS OF FICTION – NARRATOR / NARRATIVE VOICE Fundamental Literary Terms that Indentify Components of Narratives “Fiction” is defined as any imaginative re-creation of life in prose narrative form. All fiction is a falsehood of sorts because it relates events that never actually happened to people (characters) who never existed, at least not in the manner portrayed in the stories. However, fiction writers aim at creating “legitimate untruths,” since they seek to demonstrate meaningful insights into the human condition. Therefore, fiction is “untrue” in the absolute sense, but true in the universal sense. Critical Thinking – analysis of any work of literature – requires a thorough investigation of the “who, where, when, what, why, etc.” of the work. Narrator / Narrative Voice Guiding Question: Who is telling the story? …What is the … Narrative Point of View is the perspective from which the events in the story are observed and recounted. To determine the point of view, identify who is telling the story, that is, the viewer through whose eyes the readers see the action (the narrator). Consider these aspects: A. Pronoun p-o-v: First (I, We)/Second (You)/Third Person narrator (He, She, It, They] B. Narrator’s degree of Omniscience [Full, Limited, Partial, None]* C. Narrator’s degree of Objectivity [Complete, None, Some (Editorial?), Ironic]* D. Narrator’s “Un/Reliability” * The Third Person (therefore, apparently Objective) Totally Omniscient (fly-on-the-wall) Narrator is the classic narrative point of view through which a disembodied narrative voice (not that of a participant in the events) knows everything (omniscient) recounts the events, introduces the characters, reports dialogue and thoughts, and all details.
    [Show full text]
  • Why Wonder Woman Matters
    Why Wonder Woman Matters When I was a kid, being a hero seemed like the easiest thing in the world to be- A Blue Beetle quote from the DC Comics publication The OMAC Project. Introduction The superhero is one of modern American culture’s most popular and pervasive myths. Though the primary medium, the comic book, is often derided as juvenile or material fit for illiterates the superhero narrative maintains a persistent presence in popular culture through films, television, posters and other mediums. There is a great power in the myth of the superhero. The question “Why does Wonder Woman matter?” could be answered simply. Wonder Woman matters because she is a member of this pantheon of modern American gods. Wonder Woman, along with her cohorts Batman and Superman represent societal ideals and provide colorful reminders of how powerful these ideals can be.1 This answer is compelling, but it ignores Wonder Woman’s often turbulent publication history. In contrast with titles starring Batman or Superman, Wonder Woman comic books have often sold poorly. Further, Wonder Woman does not have quite the presence that Batman and Superman both share in popular culture.2 Any other character under similar circumstances—poor sales, lack of direction and near constant revisions—would have been killed off or quietly faded into the background. Yet, Wonder Woman continues to persist as an important figure both within her comic universe and in our popular consciousness. “Why does Wonder Woman matter?” To answer this question an understanding of the superhero and their primary medium, the comic book, is required, Wonder Woman is a comic book character, and her existence in the popular consciousness largely depends on how she is presented within the conventions of the comic book superhero narrative.
    [Show full text]
  • Resources for Educators, a Division of CCH Incorporated Home & School CONNECTION® May 2019 • Page 2
    Home&School ® Working Together for School Success CONNECTION May 2019 Shoally Creek Elementary SHORT NOTES Being neighborly Show your child how Summer learning traditions neighbors can depend on each other. Your child has spent the entire If a package is delivered on a rainy day school year learning new and the family isn’t home, she could things. Help him hang write a note saying she’s holding it for onto that knowledge, them. Or if someone leaves headlights and learn even more, on, knock on their door together to let by starting summer the person know. traditions like these. Everyday research STEM Olympics Sharpen your youngster’s research Boost your young- skills by challenging him to use them ster’s STEM skills with for practical purposes. Say he wants a series of household a pet or wonders why he needs to go engineering competi- to bed on time. Ask him to look into tions. For the first contest, what being a pet owner would require each person could build a catapult or how sleep affects kids. with craft sticks and rubber bands. See whose catapult can launch a ball the Growing up farthest. Next, maybe family members will on July 21 or a back-to-school celebration As your child approaches puberty, compete to engineer a boat that carries the the last weekend of summer break. she might compare herself to others. most pennies without sinking. Reading pals Explain that everyone develops at their Family celebrations For a fun way to stay in touch—and own pace. The tallest person in her Have your child use math to plan practice reading—help your youngster class right now may not be tallest special events, such as an Independence find a relative to be his reading pal.
    [Show full text]
  • See Who Attended
    Company Name First Name Last Name Job Title Country 24Sea Gert De Sitter Owner Belgium 2EN S.A. George Droukas Data analyst Greece 2EN S.A. Yannis Panourgias Managing Director Greece 3E Geert Palmers CEO Belgium 3E Baris Adiloglu Technical Manager Belgium 3E David Schillebeeckx Wind Analyst Belgium 3E Grégoire Leroy Product Manager Wind Resource Modelling Belgium 3E Rogelio Avendaño Reyes Regional Manager Belgium 3E Luc Dewilde Senior Business Developer Belgium 3E Luis Ferreira Wind Consultant Belgium 3E Grégory Ignace Senior Wind Consultant Belgium 3E Romain Willaime Sales Manager Belgium 3E Santiago Estrada Sales Team Manager Belgium 3E Thomas De Vylder Marketing & Communication Manager Belgium 4C Offshore Ltd. Tom Russell Press Coordinator United Kingdom 4C Offshore Ltd. Lauren Anderson United Kingdom 4Cast GmbH & Co. KG Horst Bidiak Senior Product Manager Germany 4Subsea Berit Scharff VP Offshore Wind Norway 8.2 Consulting AG Bruno Allain Président / CEO Germany 8.2 Consulting AG Antoine Ancelin Commercial employee Germany 8.2 Monitoring GmbH Bernd Hoering Managing Director Germany A Word About Wind Zoe Wicker Client Services Manager United Kingdom A Word About Wind Richard Heap Editor-in-Chief United Kingdom AAGES Antonio Esteban Garmendia Director - Business Development Spain ABB Sofia Sauvageot Global Account Executive France ABB Jesús Illana Account Manager Spain ABB Miguel Angel Sanchis Ferri Senior Product Manager Spain ABB Antoni Carrera Group Account Manager Spain ABB Luis andres Arismendi Gomez Segment Marketing Manager Spain
    [Show full text]
  • Humor As Cognitive Play
    Abstracts 393 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 JOHN MORREALL 27 28 Humor as Cognitive Play 29 30 31 This article assesses three traditional theories of laughter and humor: the Superi- 32 ority Theory, the Relief Theory, and the Incongruity Theory. Then, taking insights 33 from those theories, it presents a new theory in which humor is play with cognitive 34 shifts. 35 The oldest account of what we now call humor is the Superiority Theory. For 36 Plato and Aristotle laughter is an emotion involving scorn for people thought of as 37 inferior. Plato also objects that laughter involves a loss of self-control that can lead to 38 violence. And so in the ideal state described in his Republic and Laws, Plato puts 39 tight restrictions on the performance of comedy. 40 This negative assessment of laughter, humor, and comedy influenced early 41 Christian thinkers, who derived from the Bible a similar understanding of laughter 42 as hostile. The classic statement of the Superiority Theory is that of Thomas 394 Abstracts 1 Hobbes, who describes laughter as an expression of »sudden glory«. Henri Bergson’s 2 account of laughter in Le Rire incorporates a version of the Superiority Theory. 3 For any version of the Superiority Theory to be correct, two things must be true 4 when we laugh: we must compare ourselves with someone else or with our former 5 selves, and in that comparison we must judge our current selves superior.
    [Show full text]
  • Commencement Ceremony December 16, 2017 the Star-Spangled Banner
    EAS SITY T TENNESSEE STATE UNIVER Commencement Ceremony December 16, 2017 The Star-Spangled Banner Oh say, can you see, by the dawn’s early light, What so proudly we hailed at the twilight’s last gleaming? Whose broad stripes and bright stars through the perilous fight, O’er the ramparts we watched, were so gallantly streaming? And the rockets’ red glare, the bombs bursting in air, Gave proof through the night that our flag was still there. Oh say does that star-spangled banner yet wave O’er the land of the free and the home of the brave? Alma Mater In the shadow of the mountains, In thy halls we formed our friendships, Under skies of blue, Dear old college home; Stands our dear old Alma Mater, And to thee we pledge our hearts, Glorious to view, Wherever we may roam, Sound the chorus, speed it onward; Sound the chorus, speed it onward; Thee we’ll never fail! Thee we’ll never fail! Hail to thee, our Alma Mater— Hail to thee, our Alma Mater— Hail to thee, all hail! Hail to thee, all hail! Recent Faculty Retirees William H. Blanton Sandy K. Halford Roy Redman Engineering Nursing Undergraduate Programs Allied Health Sciences Joseph Borden Rhona S. Hurwitz Anna D. Roberts University School Curriculum and Instruction Management and Marketing Martha H. Bringhurst Kathryn J. Klopfenstein Michael A. Smith Nursing Undergraduate Programs Pediatrics Art and Design David O. Chastain Brent Liang Daniel J. Sobol Pediatrics Obstetrics and Gynecology Communication and Performance Balvin Chua Merry N. Miller Walter E. Stead Pharmacology Psychiatry Management and Marketing David M.
    [Show full text]
  • Exploring Trauma Through Fantasy
    IN-BETWEEN WORLDS: EXPLORING TRAUMA THROUGH FANTASY Amber Leigh Francine Shields A Thesis Submitted for the Degree of PhD at the University of St Andrews 2018 Full metadata for this thesis is available in St Andrews Research Repository at: http://research-repository.st-andrews.ac.uk/ Please use this identifier to cite or link to this thesis: http://hdl.handle.net/10023/16004 This item is protected by original copyright This item is licensed under a Creative Commons Licence https://creativecommons.org/licenses/by-nc-nd/4.0/ 4 Abstract While fantasy as a genre is often dismissed as frivolous and inappropriate, it is highly relevant in representing and working through trauma. The fantasy genre presents spectators with images of the unsettled and unresolved, taking them on a journey through a world in which the familiar is rendered unfamiliar. It positions itself as an in-between, while the consequential disturbance of recognized world orders lends this genre to relating stories of trauma themselves characterized by hauntings, disputed memories, and irresolution. Through an examination of films from around the world and their depictions of individual and collective traumas through the fantastic, this thesis outlines how fantasy succeeds in representing and challenging histories of violence, silence, and irresolution. Further, it also examines how the genre itself is transformed in relating stories that are not yet resolved. While analysing the modes in which the fantasy genre mediates and intercedes trauma narratives, this research contributes to a wider recognition of an understudied and underestimated genre, as well as to discourses on how trauma is narrated and negotiated.
    [Show full text]
  • 2019-20 Big South Presidential Honor Roll
    2019-20 BIG SOUTH PRESIDENTIAL HONOR ROLL CAMPBELL (261) Gerald Wilkes ............................................................................................................ football Kirsten Murphy ...................................................................... women’s lacrosse Zachary Adams .................................................................................................. baseball Jack Wilkes .................................................................................................................. football Olivia Ohlmann ..................................................................... women’s lacrosse Waldy Arias ............................................................................................................... baseball Michael Wooten .................................................................................................... football Shania Roehrich .................................................................. women’s lacrosse Tyler Babin ................................................................................................................. baseball Claude Churchward ................................................................................ men’s golf Kathleen Rogers ................................................................... women’s lacrosse Ryan Chasse ............................................................................................................ baseball Alejandro Cogollo ....................................................................................
    [Show full text]