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Fisheries & Aquatic Resources Program Vol. 9 No. 2 November 2010 Fisheries & Aquatic Resources Program - Midwest Region The Mission of the U.S. Fish & Wildlife Service: working with others to conserve, protect and enhance fish, wildlife, and plants and their habitats for the continuing benefit of the American people. The vision of the Service’s Fisheries Program is working with partners to restore and maintain fish and other aquatic resources at self-sustaining levels and to support Federal mitigation programs for the benefit of the American public. Implementing this vision will help the Fisheries Program do more for aquatic resources and the people who value and depend on them through enhanced partnerships, scientific integrity, and a balanced approach to conservation. Features La Crosse FHC Staff Visit the University of Notre Dame 4 Staff met with Notre Dame to discuss the Service assuming the role of eDNA sampling for Asian carp BY TERRY OTT, LA CROSSE FHC 2010 Watercraft Safety Training: The Year in Review 6 Watercraft safety training was completed by 120 students. BY DAVE WEDAN, LACROSSE FWCO Hatcheries… Not Just for Fish Anymore! Raising mudpuppies indoors during the winter has been a challenge at the 8 Genoa NFH. BY CHRIS OLDS, GENOA NFH -USFWS The Airboat Certification Course was held twice at the Horicon National Wildfife Refuge in 2010 with 14 students certified. To view other issues of “Fish Lines,” visit our website at: http://www.fws.gov/midwest/Fisheries/library/fishlines.htm 2 Fish Lines / November 2010 Table of Tontents Conservation Briefs ................................................................... 9-18 9 Annual Discussion of NESP Fish Passage Monitoring 14Neosho NFH Staff and Partners Lead “Fall 9 Efforts on the Upper Mississippi River 14PEEP Day” 2010 Vol. 9 No. 2 BY ROB SIMMONDS, CARTERVILLE FWCFWCO BY MELISSA CHEUNG, NEOSHO NFH ASSISTANT REGIONAL DIRECTOR 9 Connecting Across the Ocean 14Round Two of “Sturgeon in the Mike Weimer BY ANGELA BARAN, GENOA NFH Classroom” BY JORGE BUENING, GENOA NFH 10La Crosse Fish Health Center Solves Goldfish 15Fish and Wildlife Service - 101 To submit suggestions or comments, e-mail Mystery BY MARK STEINGRAEBER, LA CROSSE FWCO [email protected] BY COREY PUZACH, LA CROSSE FHC U.S. Fish & Wildlife Service, Midwest Region 11Sullivan Creek NFH Ships ‘Eyed” Lake Trout Eggs 15Gifts from a Far BY CRYSTAL LEGAULT ANDERSON, PENDILLS CREEK BY KAY HIVELY, FRIENDS OF THE NEOSHO NFH Fisheries & Aquatic Resources Program NFH 1 Federal Drive, Ft. Snelling, MN 55111 Phone: 612/713-5111 11La Crosse FHC Completes Health Inspection at 16Largemouth Bass Used to Study Endocrine 11Jordan River NFH 16Disruptors BY ERIC LEIS AND RYAN KATONA, LA CROSSE FHC BY ANGELA BARAN, GENOA NFH 12Green Bay FWCO Assists with Lake Whitefish 17November Shocking – Its Freezing! 12Tagging Study in Lake Michigan’s Green Bay 17 BY SCOTT YESS, LA CROSSE FWCO BY TED TRESKA, GREEN BAY FWCO 12La Crosse FHC Completes Health Inspection at 18Great Lakes Mass Marking Team Participates in 12Keweenaw Bay Tribal Fish Hatchery 18Tagging Workshop BY ERIC LEIS AND RYAN KATONA, LA CROSSE FHC BY JAMES WEBSTER, GREEN BAY FWCO 13New Water Source for the Genoa NFH 18Biologist Attends Leadership Training BY DOUG ALOISI, GENOA NFH BY COREY PUZACH, LA CROSSE FHC 13First Full Scale Auto Tagging a -USFWS/KarlaBarelt 13Success at Iron River NFH Brook trout are marked at the Jordan River BY NICK STARZL, IRON RIVER NFH National Fish Hatchery as part of the “Baby Brookies” program, where school groups help culture fish for local fishing events. Fish Lines is produced by the Fisheries and Aquatic Resources Program, Region 3, U.S. Fish & Wildlife Service, Ft. Snelling, Minne- sota. Items included are selected from monthly reports submitted by Region 3 fisheries offices. Photos included are used by permission and may be copyrighted. Equal opportunity to participate in, and benefit from programs and activities of the U.S. Fish and Wildlife Service is available to all individuals regardless of race, color, national origin, sex, age, disability, religion, sexual orientation, status as a parent and genetic information. For information contact Congressional Actions ........................................................................... 19 the U.S. Department of Interior, Office for ....................................................... Equal Opportunity, 1849 C Street N.W., Midwest Region Fisheries Divisions 20 Washington, DC 20240 Fisheries Contacts ................................................................................. 21 Fish Tails ............................................................................................. 22 Printed on 30% Recycled by Fiber Weight Paper Table of Tontents Fish Lines / November 2010 3 La Crosse FHC Staff Visit the University of Notre Dame BY TERRY OTT, LA CROSSE FHC arah Bauer and Terry Ott with the La Crosse cies were brought to North America in the early Fish Health Center (FHC) traveled to “Fight- 1970s for algae control in catfish culture ponds in the ing Irish” country, the University of Notre south. These invasive fish were accidentally intro- S st Dame in South Bend, Indiana, on October 31 to meet duced into the Mississippi River through flooding with Dr. David Lodge and his staff at the Center for anomalies in the early 1980s and have slowly been Aquatic Conservation. The meeting was to discuss making their way upstream in the Mississippi River the Fish and Wildlife Service assuming the role of basin. Now they are threatening to enter the Great environmental DNA (eDNA) sampling for Asian carp Lakes via the Chicago Sanitary and Shipping Canal, if they can bypass or navigate through the electrical barriers located upstream of Lockport, Illinois. During their visit to Notre Dame, Sarah and Terry were introduced to the eDNA surveillance project by Dr. Lodge, by discussing many facets of the project like false positives versus alternate pathways, devel- opment of new genetic markers and the possibility of developing a qPCR assay for Asian carp within the next several years. Dr. Chris- topher Jerde, -USFWS/SarahBauer postdoctoral Illumination of a polymerase chain reaction (PCR) gel in a laboratory at the University of Notre Dame. research associate and provide the Fish and Wildlife Service with back- and member of the eDNA team, talked to both of ground information on eDNA development at Notre them about the process of collection and data entry. Dame. The eDNA sampling is a type of polymerase He continued to discuss field sampling and collection chain reaction (PCR) technique used in molecular of two liter water samples, cooler controls, and data biology to amplify a single or a few copies of a piece of collection before, during and after each sample site. DNA across several orders of magnitude, generating Dr. Jerde discussed how to effectively disinfect thousands to millions of copies of a particular DNA sample gear and boats between sample collection sequence. Notre Dame eDNA sampling is a process sites. Dr. Andrew Mahon, research assistant profes- whereby genetic material is collected onto filter sor at Notre Dame, discussed sample filtration in the paper from filtering water samples taken from the laboratory and DNA extraction methods. Sarah and Chicago Sanitary and Shipping Canal, in Chicago. Terry participated in a hands-on demonstration of water filtration of the samples, observed lab equip- The genetic material is being shed by two species of ment in use during the extraction processes, and Asian carp- bighead and silver. These two carp spe- viewed biological kits necessary to perform the 4 Fish Lines / November 2010 Featured Articles extraction process. Quality Assurance/Quality Control in eDNA surveillance work was discussed in some length to assure water samples were not compromised in any way. Dr. Mahon discussed the process of how a negative and positive water sample is determined. Sarah and Terry pro- cessed and exam- ined the final product, a gel electrophoresis of silver carp at ~200 bps and bighead carp at ~300bps. At the end of the day, Sarah and -USFWS/SarahBauer Terry spent the Terry Ott filters water samples as part of the eDNA sampling procedure, which will be used to detect the presence of Asian carp genetic material. night at the famous Morris Inn on the campus of the University of Notre Dame. The inn is within walking dis- tance of all of the major campus attrac- tions like the Administration building with its’ famous golden dome to the “House that Rock Built.” The football stadium that legendary head football coach Knute Rockne had built in 1930, which produced over the decades seven Heisman trophy winners, numer- ous All Americans, and the movie “Rudy” and the “Four Horsemen.” A short walk across campus and you can literally see thousands of vigil candles burning at the Grotto of Lourdes, which was built by immigrant stone cutters during the late 1800s as a replica of the one in Lourdes, France. Or walk inside the beautiful Basilica of the Sacred Heart to observe the painted art work on the ceiling done by Vatican painter Luigi Gregori. Or just sit in a pew and gaze at the beautiful stained glass windows along the sides -USFWS/SarahBauer of the church letting the late autumn A trip to Notre Dame is not complete without experiencing some of the history such as the light in. Notre Dame is truly a special Knute Rockne statue. place to visit. For further info about the La Crosse FHC: http://www.fws.gov/midwest/LaCrosseFishHealthCenter/ Featured Articles Fish Lines / November 2010 5 2010 Watercraft Safety Training: The Year in Review BY DAVE WEDAN, LACROSSE FWCO he past year was another busy and rewarding one for the Regional Watercraft Safety In- Tstructor Team with more than 120 students successfully completing the following courses: Winter Airboat Training: This course was developed by Region 3 Instructors specifically for winter operation on snow, ice and open water. Two one-day courses were attended by regional airboat operators and instructors, the Regional Safety Team, the Region 6 Watercraft Safety Coordinator, and a Twin Cities area Fire/Rescue Team.
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