Physiochemical and Antibacterial Characterization of Fruits of Three Chilean Trees
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72 Fruits (2), 87–96 | ISSN 0248-1294 print, 1625-967X online | https://doi.org/10.17660/th.2017/72.2.4 | © ISHS 2017 Original article Citronella mucronata (Cardiopteridaceae), Pitavia punctata (Rutaceae)Physiochemical and Beilschmiediaand antibacterial berteroana characterization (Lauraceae), of fruits three of endemic and threatened Chilean trees , G.F. Narváez2, M.F. Morales3 3 4 and C.R. Figueroa 1 5,a F.A.12 Sáez , H.M. Bello , C. Balbontín 3 Master Program in Forest Sciences, Faculty of Forest Sciences, University of Concepción, Concepción, Chile 4 Faculty of Forest Sciences, University of Concepción, Concepción, Chile Research Lab of Antibacterial Agents, Faculty of Biological Sciences, University of Concepción, Concepción, Chile 5 Small Fruits and Berry Crops Research, Institute for Agricultural Research (INIA)-Quilamapu, Chillán, Chile Phytohormone Research Laboratory, Institute of Biological Sciences, University of Talca, Talca, Chile Summary Significance of this study Introduction – Several native tree species are What is already known on this subject? scarcely studied in relation to fruit properties. In or- • Citronella mucronata, Pitavia punctata and Beilschmie- der to bring about information of these plant resourc- dia berteroana are threatened endemic trees of central es, the characterization of ripening-associated prop- erties of the fruit of three endemic and threatened studied. Chilean trees (Citronella mucronata, Pitavia punctata Chile whose fruit properties have been scarcely and Beilschmiedia berteroana) was performed in the What are the new findings? present study. Materials and methods – The physio- • C. mucronata and P. punctata chemical characterization of two developmental fruit a high amount of pectin and bacteriostatic effect, stages in each species included the measurement of fruits extracts showed soluble solid content (SSC), titratable acidity (TA), pH, for both fruits. color and chlorophyll content; cell wall composition respectively. Harvest indexes were described and lignin content; the total phenolic content and What is the expected impact on horticulture? antioxidant capacity of the fruit extracts. Moreover, • the minimal inhibitory concentration (MIC) was car- used for food and pharmaceutical industries. ried out in fruit extracts against 11 bacterial strains. Under a sustainable management, these fruits can be Results and discussion – Pitavia punctata and B. bertero- ana fruits exhibited similar SSC and TA values showing a significant increase of the SSC:TA ratio during devel- Résumé opment. However, C. mucronata showed the highest SSC value at stage II with 8.1 °Brix, accompanied by riennes des fruits de Citronella mucronata a noticeable color change. The analysis of pectin frac- pteridaceae),Caractéristiques de Pitaviaphysico-chimiques punctata (Rutaceae) et anti-bacté et de- tions suggests that solubilisation of pectic polymers (Cardio- increase during fruit development in all species. The Beilschmiedia berteroana (Lauraceae), trois arbres fruits of C. mucronata presented the highest level of total uronic acids (for stage I and II, a 63% and 71% of Introduction – Nombreuses sont les espèces the cell wall material, respectively). The phenolic con- d’arbreschiliens endémiquesindigènes dont et menacés.les propriétés des fruits sont tent, antioxidant capacity and MIC were particularly peu étudiées. Afin d’obtenir des informations sur ces remarkable in P. punctata fruits at stage II, when fruit ressources végétales, l’étude présente a caractérisé les reaches 6.5 °Brix, with values of 924.4 mg GAE 100 g-1 propriétés associées au stade de maturité des fruits -1 FW, 2.51 mM FeSO4 g FW, and 6.25% v/v, respective- de trois arbres chiliens endémiques et menacés (Ci- ly. Conclusion – These results relate interesting prop- tronella mucronata, Pitavia punctata et Beilschmiedia erties of C. mucronata and P. punctata fruits for food berteroana). Matériel et méthodes – La caractérisation and pharmaceutical industries with physiological in- physicochimique de deux stades de développement dexes at stage II, mainly soluble solids and color. de chaque espèce comprenait les mesures suivantes: la teneur en matières solubles (SSC), l’acidité titrable Keywords (TA), le pH, la couleur et la teneur en chlorophylle des extraits de ces fruits, ainsi que la composition des pa- rois cellulaires, les teneurs en lignine, en composés Chile, endemic species, non-timber forest products, phénoliques totaux et la capacité anti-oxydante. De underutilized species, fruit quality, cell wall composition, plus, la concentration inhibitrice minimale (CMI) des antioxidant compounds a Corresponding author: extraits de ces fruits a été effectuée contre 11 souches or [email protected] [email protected]. Volume 72 | Issue 2 | March/April 2017 87 Sáez et al. | Physiochemical and antibacterial characterization of fruits of three Chilean trees bactériennes. Résultats et discussion – Les fruits de P. et al Pitavia punctata is a perennial tree belonging punctata et de B. berteroana ont présenté des valeurs to1.2 the cm Rutaceae diameter, dark purple at ripe stage (Hechenleitner semblables de SSC et de TA, montrant une augmenta- ., 2005). tion significative du rapport SSC: TA au cours de leur for antimicrobial family, properties and its (Esterhuizen potential could et al be., 2006).similar to développement. Cependant, C. mucronata a montré other species of the family which have been characterized la plus grande valeur de SSC au stade II avec 8,1 °Brix P. punctata Anet accompagné d’un changement notable de coloration. alearly characterizationP. ofpunctata extracts from stems and leaves of L’analyse des fractions de pectine suggère que la so- reported several flavonoid compounds (Silva lubilisation des polymères pectiques augmente au ., 1971). Fruits of are ovoid drupeset al (1.8–2.5 cours du développement des fruits chez toutes les es- Beilschmiediacm diameter) berteroanagreen at the is anfirst evergreen developmental tree that stages belongs and to pèces étudiées. Les fruits de C. mucronata ont présen- theyellowish Lauraceae green at ripe stage (Hechenleitner ., 2005). té le niveau le plus élevé d’acides uroniques totaux (2×2 cm) (Hechenleitner et al (pour les stades I et II, respectivement 63% et 71% de about the fruit family properties with globular of this species and greyish-yellow is scarce, however drupes la paroi cellulaire). Le contenu phénolique, la capaci- ., 2005). The information té anti-oxydante et la CMI ont été particulièrement re- et al marquables dans les fruits de P. punctata au stade II, the fruits could be interesting since they would present high quand les fruits atteignent 6,5 °Brix, avec des valeurs speciesamounts has of mucilagenot been reported.(Rodríguez Chilean ., 1983).native fruits have the -1 -1 de 924,4 mg GAE 100 g FW, 2,51 mM FeSO4 g FW et potentialThe characterization to become in appreciated of fruit properties supplies for in nutraceuticalthis group of 6,25% v/v, respectivement. Conclusion – Ces résultats rapportent des propriétés particulières des fruits de Aristotelia chilensis Ugni C. mucronata et P. punctata peuvent intéresser l’in- molinaeindustry. In this sense, extracts fromLuma the apiculata Chilean native berries dustrie alimentaire ou pharmaceutique avec des va- ‘maqui’ ( [Molina] Stuntz), ‘murtilla’ ( leurs de référence au stade II du développement des Turcz.) and ‘arrayan’ ( [DC.] Burret) fruits, surtout en matières solubles et coloration. duehave to shown the phenolic interesting composition results forof theirtheir leavesantioxidant and fruits and antibacterial activitieset al and potential ethealth al benefits, mainlyet Mots-clés al (Genskowsky ., 2016; Suwalsky ., 2007; Fuentes pectin,., 2016). a main Nevertheless, component other of the interesting cell wall metabolitesfound in a wide and Chili, espèces endémiques, produits forestiers non-ligneux, polymers could be found in endemic fruits. For instance, espèces sous-utilisées, qualité du fruit, composition des and pharmaceutical industries (Willats et al., 2006). In parois cellulaires, composés anti-oxydants variety of fruits, is a valuable ingredient for food, cosmetic processes can be described in indigenous or underutilized Introduction fruitsaddition, that othercould helpinteresting understand and theparticular ripening physiological phenomena, Fragaria chiloensis [L.] Mill.) (Cherian et al Chile iset considered al a ‘biogeographic island’, with diverse as in the case of the Chilean strawberry ( climates, ecosystems, and an extraordinary biodiversity potential of Citronella., 2014).mucronata, Pitavia punctata and (Armesto ., 1998). While the diversity of native plants Chile has a high percentage of endemism what makes it a BeilschmiediaThe objective berteroana of this fruits work as materialswas to fordetermine the food andthe may be low, considering with otheret Southal., 2000). American However, countries, there of‘hotspot’ these endemic of biodiversity resources (Myers (Hechenleitner et al pharmaceutical industries. The characterization included is little information about the biology and usefulness of many the analysis of physiochemical and antimicrobial properties sustainable management of forests and their .,species 2005). withThis of the fruits. In a first step, fruit quality parameters such as conservationsituation constitutes problems. a major While economicforests have opportunity been studied for for the a color, solid soluble concentration (SSC) and titratable acidity long time under the approach of obtaining wood