Biologia 63/3: 349—367, 2008 Section Botany DOI: 10.2478/s11756-008-0052-9 DNA ploidy level variability of some fescues (Festuca subg. Festuca, Poaceae) from Central and Southern Europe measured in fresh plants and herbarium specimens Petr Šmarda Masaryk University, Faculty of Science, Institute of Botany and Zoology, Kotlářská 2,CZ-61137 Brno, Czech Republic; e-mail:
[email protected]. Abstract: Using flow cytometry in fresh plants and herbarium vouchers, DNA ploidy levels for 411 individuals of 44 taxa of the genus Festuca, including 4 natural hybrids, originating from 237 sites in Austria, Bulgaria, Croatia, Czech Republic, Estonia, Germany, Hungary, Italy, Poland, Romania, Slovakia, Slovenia, and Switzerland were estimated. The following taxa and DNA ploidy levels are reported: F. airoides (2n ≈ 2x), F. alpestris (2n ≈ 2x), F. alpina s.l. (2n ≈ 2x), F. amethystina subsp. amethystina (2n ≈ 4x), F. bosniaca subsp. bosniaca (2n ≈ 2x), F. brevipila (2n ≈ 6x), F. bucegiensis (2n ≈ 2x), F. carnuntina (2n ≈ 6x), F. csikhegyensis (2n ≈ 4x), F. csikhegyensis × F. eggleri (2n ≈ 4x), F. dalmatica (2n ≈ 4x), F. duvalii (2n ≈ 4x), F. eggleri (2n ≈ 2x,4x), F. filiformis (2n ≈ 2x), F. glauca (2n ≈ 6x), F. heterophylla (2n ≈ 4x), F. inops (2n ≈ 2x), F. laevigata (2n ≈ 8x), F. laxa (2n ≈ 4x), F. lemanii (2n ≈ 6x), F. norica (2n ≈ 2x), F. ovina subsp. ovina (2n ≈ 2x), F. ovina subsp. guesfalica (2n ≈ 4x), F. ovina × F. pallens (2n ≈ 4x), F. pallens (2n ≈ 2x,3x), F. pallens × F. pseudodalmatica (2n ≈ 3x,4x), F. pirinica (2n ≈ 2x), F. polesica (2n ≈ 2x), F. psammophila subsp. dominii (2n ≈ 2x), F. pseudodalmatica (2n ≈ 4x), F.