1 Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake 2 3 Miroslava Soldánová a,†, Simona Georgieva a,†, Jana Roháčová a,b, Rune Knudsen c, Jesper A. 4 Kuhn c, Eirik H. Henriksen c, Anna Siwertsson c, Jenny C. Shaw d, Armand M. Kuris d, Per-Arne 5 Amundsen c, Tomáš Scholz a,b, Kevin D. Lafferty e, Aneta Kostadinova a,* 6 7 a Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05 8 České Budějovice, Czech Republic 9 b Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, 10 Czech Republic 11 c Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, 12 UiT The Arctic University of Norway, N9037 Tromsø, Norway 13 d Department of Ecology, Evolution and Marine Biology and Marine Science Institute, University 14 of California, Santa Barbara, California 93106 USA 15 e United States Geological Survey, Western Ecological Research Center c/o Marine Science 16 Institute, University of California, Santa Barbara, California 93106 USA 17 18 † Equal contributors 19 * Corresponding author. Tel.: +420-38-777 5933; fax: +420-38-5310388. 20 E-mail address:
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[email protected] 21 22 23 24 25 26 Note: Supplementary data associated with this article. 27 28 29 International Journal for Parasitology 47 (2017) 327–345 30 http://dx.doi.org/10.1016/j.ijpara.2016.12.008 1 31 ABSTRACT 32 To identify trematode diversity and life-cycles in the sub-Arctic lake Takvatn, we characterised 33 120 trematode isolates from mollusc first intermediate hosts and metacercariae from second 34 intermediate host fishes and invertebrates using molecular techniques.