DOCSLIB.ORG

Invert4 2 139 149 Tolstenkov.PM6

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Invert4 2 139 149 Tolstenkov.PM6 Çîîëîãèÿ áåñïîçâîíî÷íûõ, 2007, 4(2): 139149 © ÇÎÎËÎÃÈß ÁÅÑÏÎÇÂÎÍÎ×ÍÛÕ, 2007 Îðãàíèçàöèÿ ìûøå÷íîé ñèñòåìû è ðàñïðåäåëåíèå NO-åðãè÷åñêèõ è ñåðîòîíèíåðãè÷åñêèõ ýëåìåíòîâ ó òðåìàòîä Allocreadium isoporum Looss, 1894 (Allocreadiidae) è Paramphistomum cervi Zeder, 1790 (Paramphistomatidae) Î.Î. Òîëñòåíêîâ1, Í.Á. Òåðåíèíà1, Í.Ì. Øàëàåâà2, Ò.Â. Ãàéâîðîíñêàÿ1 1 Öåíòð ïàðàçèòîëîãèè ôèëèàë Èíñòèòóòà ïðîáëåì ýêîëîãèè è ýâîëþöèè èìåíè À.Í. Ñåâåðöîâà ÐÀÍ, Ëåíèíñêèé ïðîñïåêò 33, 119071 Ìîñêâà, Ðîññèÿ. e-mail: [email protected] 2 Êàôåäðà çîîëîãèè áåñïîçâîíî÷íûõ Áèîëîãè÷åñêîãî ôàêóëüòåòà Ìîñêîâñêîãî Ãîñóäàð- ñòâåííîãî Óíèâåðñèòåòà, Âîðîáüåâû ãîðû, 119991 Ìîñêâà, Ðîññèÿ. ÐÅÇÞÌÅ: Ñ èñïîëüçîâàíèåì TRITC-ìå÷åííîãî ôàëëîèäèíà èçó÷åíà àíàòîìè÷åñêàÿ îðãàíèçàöèÿ ìûøå÷íîé ñèñòåìû ó òðåìàòîä Allocreadium isoporum è Paramphistomum cervi. Èññëåäîâàíî ðàñïðåäåëåíèå NO-åðãè÷åñêèõ è 5-ÍÒ-åðãè÷åñêèõ ýëåìåíòîâ â íåðâíîé ñèñòåìå è ìóñêóëàòóðå. Ñòðóêòóðû, ãåíåðèðóþùèå îêñèä àçîòà, è 5-ÍÒ- åðãè÷åñêèå ýëåìåíòû íàéäåíû â öåíòðàëüíîé è ïåðèôåðè÷åñêîé íåðâíîé ñèñòåìå îáîèõ âèäîâ. Íàëè÷èå NO-åðãè÷åñêèõ ñòðóêòóð è 5-ÍÒ-åðãè÷åñêèõ ýëåìåíòîâ â òîëùå ìóñêóëàòóðû ñâèäåòåëüñòâóåò îá ó÷àñòèè îêñèäà àçîòà è ñåðîòîíèíà â ðåãóëÿ- öèè ñîêðàòèòåëüíîé àêòèâíîñòè òðåìàòîä. ÊËÞ×ÅÂÛÅ ÑËÎÂÀ: òðåìàòîäû, Allocreadium isoporum, Paramphistomum cervi, ìóñêóëàòóðà, íåðâíàÿ ñèñòåìà, íåéðîìåäèàòîðû, îêñèä àçîòà, ñåðîòîíèí. The organization of the muscular system and the distribution of NO-ergic and serotoninergic elements of trematodes Allocreadium isoporum Looss, 1894 (Allocreadiidae) and Paramphistomum cervi Zeder, 1790 (Paramphistomatidae) O.O. Tolstenkov1, N.B. Terenina1, N.M. Shalaeva2, T.V. Gaivoronskaja1 1 Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Science, Leninskiy prospect 33, 119071 Moscow,, Russia. e-mail: [email protected] 2 Department of Invertebrate Zoology, Biological Faculty, Moscow State University, Vorobievy Gori, 119899 Moscow, Russia ABSTRACT: TRITC-conjugated phalloidin staining was used to investigate the anatomical organization of the musculature of Allocreadium isoporum and Paramphistomum cervi. 140 Î.Î. Òîëñòåíêîâ, Í.Á. Òåðåíèíà, Í.Ì. Øàëàåâà, Ò.Â. Ãàéâîðîíñêàÿ The distribution of NO-ergic and 5-HT-ergic elements in the nervous system and the musculature was studied. Structures generating NO and 5-HT-ergic elements were observed in the central and peripheral nervous system of both species. The presence of NO-ergic and 5-HT-ergic nerve elements in the musculature testified the participation of Nitric oxide and serotonin in the regulation of the motility of trematodes. KEY WORDS: Trematoda, Allocreadium isoporum, Paramphistomum cervi, musculature, nervous system, neuromediators, nitric oxide, serotonin. Ââåäåíèå Ìîñêîâñêîé îáëàñòè. Ìàòåðèàë ôèêñèðîâà- ëè â 4%-íîì ïàðàôîðìàëüäåãèäå â 0,01 Ì Ïðèìåíåíèå ãèñòîõèìè÷åñêèõ è èììó- ôîñôàòíîì áóôåðíîì ðàñòâîðå (ðH 7,4) ïðè íîöèòîõèìè÷åñêèõ ìåòîäîâ ïîçâîëèëî âûÿ- 4°Ñ è çàòåì ñîõðàíÿëè â 10%-íîé ñàõàðîçå, âèòü â íåðâíîé ñèñòåìå ãåëüìèíòîâ õîëè- ïðèãîòîâëåííîé íà 0,01 Ì ôîñôàòíîì áóôå- íåðãè÷åñêèå, ñåðîòîíèíåðãè÷åñêèå, ïåïòè- ðå. ×àñòü ìàòåðèàëà áûëà èñïîëüçîâàíà äëÿ äåðãè÷åñêèå ýëåìåíòû (Øèøîâ, 1991; Barton ïðèãîòîâëåíèÿ êðèîñòàòíûõ ñðåçîâ òîëùè- et al., 1993; Biserova et al., 1996; Biserova et íîé 20 ìêì, äðóãóþ ÷àñòü èñïîëüçîâàëè äëÿ al., 2000; Gzubaj, Grabda-Kazubska, 1993; îêðàøèâàíèÿ öåëûõ ïàðàçèòîâ. Marks et al., 1995; Niewiadomska et al., 1996 è Îêðàñêó ìóñêóëàòóðû ïðîâîäèëè ñ èñ- äð.). Îòêðûòèå ôóíêöèè îêñèäà àçîòà êàê ïîëüçîâàíèåì TRITC (òåòðàìåòèëðîäàìèí àòèïè÷íîãî íåéðîíàëüíîãî ìåññåíäæåðà â èçîòèîöèàíàò) ìå÷åííîãî ôàëëîèäèíà (â íåðâíîé ñèñòåìå ìëåêîïèòàþùèõ (Garth- ðàçâåäåíèè 1:200) âî âëàæíîé êàìåðå â òå÷å- waite, 1991; Snyder, 1992) äàëî òîë÷îê ê èñ- íèå 1 ÷ â òåìíîòå ïðè òåìïåðàòóðå 4°C â ñëåäîâàíèþ ýòîãî âåùåñòâà íå òîëüêî ó ìëå- ñîîòâåòñòâèè ñ ìåòîäîì, îïèñàííûì Wahl- êîïèòàþùèõ, íî è ó äðóãèõ æèâîòíûõ, â òîì berg (1998). ÷èñëå è áåñïîçâîíî÷íûõ.  ïîñëåäíèå ãîäû Äëÿ èäåíòèôèêàöèè íåðâíûõ ñòðóêòóð, ïîÿâèëèñü äàííûå î íàëè÷èè ñòðóêòóð, ãåíå- ñîäåðæàùèõ ñèíòàçó îêñèäà àçîòà ôåð- ðèðóþùèõ îêñèä àçîòà, ó ãåëüìèíòîâ (Gus- ìåíò ñèíòåçà îêñèäà àçîòà (NO-ñèíòàçó), tafsson et al., 2001; Terenina, Gustafsson, 2002; èñïîëüçîâàëè ÍÀÄÔÍ (íèêîòèíàìèäàäåíèí- Terenina et al., 2006). äèíóêëåîòèäôîñôàò, âîññòàíîâëåííàÿ ôîð-  íàñòîÿùåé ðàáîòå ïðèâåäåíû ðåçóëü- ìà)-äèàôîðàçíûé (ÍÀÄÔÍ-ä) ãèñòîõèìè- òàòû èçó÷åíèÿ ìûøå÷íîé ñèñòåìû, à òàêæå ÷åñêèé ìåòîä. Ìåòîä îñíîâàí íà îïðåäåëå- äàííûå èììóíîöèòîõèìè÷åñêîãî è ãèñòîõè- íèè ôåðìåíòà ÍÀÄÔÍ-äèàôîðàçû, èçâåñò- ìè÷åñêîãî èññëåäîâàíèÿ ñåðîòîíèíåðãè÷åñ- íîãî êàê ìàðê¸ð NO-ñèíòàçû, ñîãëàñíî ñòàí- êèõ íåðâíûõ ñòðóêòóð è ñòðóêòóð, ãåíåðèðó- äàðòíîé ìåòîäèêå (Hope, Vincent, 1989). þùèõ îêñèä àçîòà (NO-åðãè÷åñêèõ, íèòðî- Ëîêàëèçàöèþ ñåðîòîíèíåðãè÷åñêèõ (5- êñèäåðãè÷åñêèõ), ó äâóõ âèäîâ òðåìàòîä: ÍÒ-åðãè÷åñêèõ) íåðâíûõ ñòðóêòóð îïðåäå- Allocreadium isoporum Looss, 1894 (ñåì. Allo- ëÿëè èììóíîöèòîõèìè÷åñêè â ñîîòâåòñòâèè creadiidae) è Paramphistomum cervi Zeder, ñ ìåòîäîì Coons et al. (1955). Îêðàøèâàíèå 1790 (ñåì. Paramphistomatidae). ïðîâîäèëè íà êðèîñòàòíûõ ñðåçàõ è òîòàëü- íûõ ïðåïàðàòàõ. Ñðåçû èíêóáèðîâàëè â ïåð- âè÷íîé àíòèñûâîðîòêå (Incstar, USA, â ðàç- Ìàòåðèàë è ìåòîäû âåäåíèè 1:500) 48 ÷ ïðè òåìïåðàòóðå 4°C; âî âòîðè÷íîé àíòèñûâîðîòêå (TRITC, DAKO, â Âçðîñëûå ôîðìû A. isoporum áûëè âçÿòû ðàçâåäåíèè 1:30) ïðåïàðàòû èíêóáèðîâàëè â èç êèøå÷íèêà óêëåè (Alburnus alburnus), òå÷åíèå 3 ÷. ïîéìàííîé â âîäîåìàõ Êàðåëèè, P. cervi Äëÿ ñîïîñòàâëåíèÿ ÍÀÄÔÍ-ä ñòðóêòóð, èç ðóáöà äîìàøíåé êîðîâû (Bos taurus) â ñòðóêòóð, ñîäåðæàùèõ ñåðîòîíèí, è ìûøå÷- Îðãàíèçàöèÿ ìûøå÷íîé ñèñòåìû ó òðåìàòîä 141 íûõ âîëîêîí áûëî ïðîâåäåíî òðîéíîå îêðà- öåâûìè âîëîêíàìè. Âåòâè êèøå÷íèêà â ðàé- øèâàíèå ñðåçîâ: ÍÀÄÔÍ-äèàôîðàçíûì ãè- îíå áèôóðêàöèè è äî èçãèáà â êàóäàëüíîì ñòîõèìè÷åñêèì ìåòîäîì, çàòåì èììóíîöè- íàïðàâëåíèè òàêæå èìåþò äâà ìûøå÷íûõ ñëîÿ òîõèìè÷åñêîå îêðàøèâàíèå íà ñåðîòîíèí è ìåíåå ðàçâèòûé íàðóæíûé ïðîäîëüíûé è îêðàñêà ìûøå÷íûõ âîëîêîí ñ èñïîëüçîâà- âíóòðåííèé êîëüöåâîé.  öåëîì, ìóñêóëàòó- íèåì TRITC-ìå÷åííîãî ôàëëîèäèíà. ðà êèøå÷íèêà ñòàíîâèòñÿ ìåíåå âûðàæåííîé Ïðåïàðàòû èññëåäîâàëè ñ ïîìîùüþ êîí- ïî ìåðå óäàëåíèÿ îò ìåñòà áèôóðêàöèè. ôîêàëüíîãî ñêàíèðóþùåãî ëàçåðíîãî ìèê-  ïîëîâîé ñèñòåìå A. isoporum ìûøå÷- ðîñêîïà Leica TCS 4D, ñîåäèíåííîãî ñ ôëó- íûé ñëîé íàèáîëåå ñèëüíî ðàçâèò â îáëàñòè îðåñöåíòíûì ìèêðîñêîïîì Leitz Aristoplan, åå äèñòàëüíûõ îòäåëîâ. Äèñòàëüíûé îòäåë à òàêæå ìèêðîñêîïà Axiostar plus, Zeiss. ìàòêè ìåòðàòåðì, à òàêæå äèñòàëüíûå îòäåëû ìóæñêîé ïîëîâîé ñèñòåìû èìåþò Ðåçóëüòàòû òîëñòóþ ìûøå÷íóþ ñòåíêó, ñîñòîÿùóþ èç õîðîøî ðàçâèòîãî êîëüöåâîãî ñëîÿ è ìåíåå Allocreadium isoporum. Ìóñêóëàòóðà ìîùíîãî ïðîäîëüíîãî ñëîÿ (ðèñ. 1D). Êîëü- ñòåíêè òåëà A. isoporum ïðåäñòàâëåíà òðåìÿ öåâûå è ïðîäîëüíûå ìûøå÷íûå âîëîêíà ñëîÿìè ìûøå÷íûõ âîëîêîí: íàðóæíûì êîëü- îêðóæàþò ïîëîâîå îòâåðñòèå, îòêðûâàþùå- öåâûì, ïðîìåæóòî÷íûì ïðîäîëüíûì, à òàê- åñÿ ìåäèàííî. Îîòèï èìååò óòîëùåííóþ æå ñëîåì âíóòðåííèõ äèàãîíàëüíûõ âîëî- ìûøå÷íóþ ñòåíêó, ñîñòîÿùóþ èç êîëüöå- êîí (ðèñ. 1À). Êîëüöåâûå âîëîêíà îðèåíòè- âûõ è ïðîäîëüíûõ ìûøå÷íûõ âîëîêîí. Ïðî- ðîâàíû â îñíîâíîì ïîä ïðÿìûì óãëîì ê êñèìàëüíûå îòäåëû ïîëîâîé ñèñòåìû èìå- ïðîäîëüíîé îñè òåëà. Îíè ÿâëÿþòñÿ ñàìûìè þò òîíêóþ ìûøå÷íóþ ñòåíêó, ñîñòîÿùóþ òîíêèìè è ìíîãî÷èñëåííûìè, ðàñïîëîæåíû ïðåèìóùåñòâåííî èç êîëüöåâûõ âîëîêîí. ÷àùå äðóãèõ. Ïðîäîëüíûå âîëîêíà èìåþò Ïðè èññëåäîâàíèè NO-åðãè÷åñêèõ ñòðóê- áîëüøóþ òîëùèíó, ÷åì êîëüöåâûå, è ðàñïî- òóð ó A. isoporum îáíàðóæåíî, ÷òî ÍÀÄÔÍ- ëîæåíû ðåæå. Òîëñòûå äèàãîíàëüíûå âîëîê- äèàôîðàçíàÿ ðåàêöèÿ âûÿâëÿåòñÿ ïî áîêàì íà ðàñïîëàãàþòñÿ ïàðàìè è ïåðåñåêàþòñÿ ãëîòêè, íà óðîâíå âåðõíåé åå ÷àñòè, ò.å. â ïîä óãëîì 120150°. îáëàñòè öåðåáðàëüíûõ ãàíãëèåâ (ðèñ. 1E). Ìîùíî ðàçâèòàÿ ìóñêóëàòóðà ðîòîâîé è NO-åðãè÷åñêèå âîëîêíà âèäíû â îòõîäÿùèõ áðþøíîé ïðèñîñîê ïðåäñòàâëåíà òðåìÿ òè- îò öåðåáðàëüíûõ ãàíãëèåâ ïðîäîëüíûõ íå- ïàìè ìûøå÷íûõ âîëîêîí: ðàäèàëüíûìè, ðâíûõ ñòâîëàõ, êîòîðûå ïðîñëåæèâàþòñÿ íà êîëüöåâûìè è ïðîäîëüíûìè. Êîëüöåâûå âî- ñðåçàõ äî óðîâíÿ áðþøíîé ïðèñîñêè.  äè- ëîêíà îáðàçóþò âíåøíèé ìûøå÷íûé ñëîé ñòàëüíîé ÷àñòè òåëà ïàðàçèòà ïîëîæèòåëü- ñòåíêè ïðèñîñêè, ïîä íèìè ðàñïîëàãàåòñÿ íîå îêðàøèâàíèå ïðîäîëüíûõ íåðâíûõ ñòâî- òîíêèé ñëîé ïðîäîëüíûõ âîëîêîí, ðàäèàëü- ëîâ íå âûÿâëåíî. Ñïåöèôè÷åñêè îêðàøåí- íûå âîëîêíà ïðîõîäÿò îò âíóòðåííåé ê âíåø- íûå âîëîêíà îòìå÷åíû ìåæäó ðàäèàëüíûìè íåé ñòåíêå ïðèñîñêè. Îò ñòåíêè òåëà ê áðþø- ìûøå÷íûìè âîëîêíàìè áðþøíîé è ðîòî- íîé ïðèñîñêå ñ ÷åòûðåõ ñòîðîí èäóò ïó÷êè âîé ïðèñîñîê â ñóáòåãóìåíòå. Ïîìèìî ýòî- ìûøå÷íûõ âîëîêîí (ðèñ. 1B). Ìóñêóëèñòàÿ ãî, ñðåäè ìûøå÷íûõ âîëîêîí ðîòîâîé è ãëîòêà èìååò øàðîîáðàçíóþ ôîðìó è âêëþ- áðþøíîé ïðèñîñîê, à òàêæå ãëîòêè îáíàðó- ÷àåò äâà ñëîÿ ìûøå÷íûõ âîëîêîí: õîðîøî æåíû íåñêîëüêî ðàäèàëüíî ðàñïîëîæåííûõ ðàçâèòûé ðàäèàëüíûé ñëîé è êîëüöåâîé ñëîé ñòðóêòóð, èìåþùèõ ïîëîæèòåëüíóþ ðåàê- (ðèñ. 1C). Íà ãðàíèöå ðîòîâîé ïðèñîñêè è öèþ íà ÍÀÄÔÍ-äèàôîðàçó (ðèñ. 1F). Ðåàê- ïðåôàðèíêñà èìååòñÿ ñóæåíèå òèïà ñôèíêòå- öèÿ íàáëþäàëàñü òàêæå â âîëîêíàõ, ðàñïî- ðà, îãðàíè÷åííîå êîëüöåâûìè âîëîêíàìè. ëîæåííûõ âáëèçè áðþøíîé ïðèñîñêè. Ïèùåâîä èìååò ðàçâèòûé ìûøå÷íûé ñëîé, Ñèëüíîå ïîëîæèòåëüíîå îêðàøèâàíèå íà ïðåäñòàâëåííûé õîðîøî âûðàæåííûìè íà- ÍÀÄÔÍ-äèàôîðàçó îáíàðóæåíî â äèñòàëü- ðóæíûìè ïðîäîëüíûìè è âíóòðåííèìè êîëü- íûõ è ïðîêñèìàëüíûõ îòäåëàõ ìóæñêîé è 142 Î.Î. Òîëñòåíêîâ, Í.Á. Òåðåíèíà, Í.Ì. Øàëàåâà, Ò.Â. Ãàéâîðîíñêàÿ Îðãàíèçàöèÿ ìûøå÷íîé ñèñòåìû ó òðåìàòîä 143 æåíñêîé ïîëîâîé ñèñòåìû: â ìûøå÷íîé ñòåí- òóðó è ñîñòîÿò èç òîíêîãî ñëîÿ ïðîäîëüíûõ êå ñåìÿèçâåðãàòåëüíîãî êàíàëà, à òàêæå â è êîëüöåâûõ âîëîêîí, à òàêæå ìíîãî÷èñëåí-
Load more

Recommended publications
  • Review and Meta-Analysis of the Environmental Biology and Potential Invasiveness of a Poorly-Studied Cyprinid, the Ide Leuciscus Idus
    REVIEWS IN FISHERIES SCIENCE & AQUACULTURE https://doi.org/10.1080/23308249.2020.1822280 REVIEW Review and Meta-Analysis of the Environmental Biology and Potential Invasiveness of a Poorly-Studied Cyprinid, the Ide Leuciscus idus Mehis Rohtlaa,b, Lorenzo Vilizzic, Vladimır Kovacd, David Almeidae, Bernice Brewsterf, J. Robert Brittong, Łukasz Głowackic, Michael J. Godardh,i, Ruth Kirkf, Sarah Nienhuisj, Karin H. Olssonh,k, Jan Simonsenl, Michał E. Skora m, Saulius Stakenas_ n, Ali Serhan Tarkanc,o, Nildeniz Topo, Hugo Verreyckenp, Grzegorz ZieRbac, and Gordon H. Coppc,h,q aEstonian Marine Institute, University of Tartu, Tartu, Estonia; bInstitute of Marine Research, Austevoll Research Station, Storebø, Norway; cDepartment of Ecology and Vertebrate Zoology, Faculty of Biology and Environmental Protection, University of Lodz, Łod z, Poland; dDepartment of Ecology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia; eDepartment of Basic Medical Sciences, USP-CEU University, Madrid, Spain; fMolecular Parasitology Laboratory, School of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston-upon-Thames, Surrey, UK; gDepartment of Life and Environmental Sciences, Bournemouth University, Dorset, UK; hCentre for Environment, Fisheries & Aquaculture Science, Lowestoft, Suffolk, UK; iAECOM, Kitchener, Ontario, Canada; jOntario Ministry of Natural Resources and Forestry, Peterborough, Ontario, Canada; kDepartment of Zoology, Tel Aviv University and Inter-University Institute for Marine Sciences in Eilat, Tel Aviv,
    [Show full text]
  • Synopsis of the Parasites of Fishes of Canada
    1 ci Bulletin of the Fisheries Research Board of Canada DFO - Library / MPO - Bibliothèque 12039476 Synopsis of the Parasites of Fishes of Canada BULLETIN 199 Ottawa 1979 '.^Y. Government of Canada Gouvernement du Canada * F sher es and Oceans Pëches et Océans Synopsis of thc Parasites orr Fishes of Canade Bulletins are designed to interpret current knowledge in scientific fields per- tinent to Canadian fisheries and aquatic environments. Recent numbers in this series are listed at the back of this Bulletin. The Journal of the Fisheries Research Board of Canada is published in annual volumes of monthly issues and Miscellaneous Special Publications are issued periodically. These series are available from authorized bookstore agents, other bookstores, or you may send your prepaid order to the Canadian Government Publishing Centre, Supply and Services Canada, Hull, Que. K I A 0S9. Make cheques or money orders payable in Canadian funds to the Receiver General for Canada. Editor and Director J. C. STEVENSON, PH.D. of Scientific Information Deputy Editor J. WATSON, PH.D. D. G. Co«, PH.D. Assistant Editors LORRAINE C. SMITH, PH.D. J. CAMP G. J. NEVILLE Production-Documentation MONA SMITH MICKEY LEWIS Department of Fisheries and Oceans Scientific Information and Publications Branch Ottawa, Canada K1A 0E6 BULLETIN 199 Synopsis of the Parasites of Fishes of Canada L. Margolis • J. R. Arthur Department of Fisheries and Oceans Resource Services Branch Pacific Biological Station Nanaimo, B.C. V9R 5K6 DEPARTMENT OF FISHERIES AND OCEANS Ottawa 1979 0Minister of Supply and Services Canada 1979 Available from authorized bookstore agents, other bookstores, or you may send your prepaid order to the Canadian Government Publishing Centre, Supply and Services Canada, Hull, Que.
    [Show full text]
  • Trematoda: Allocreadiidae) by Means of 28S Ribosomal DNA Sequences
    Advances in Bioscience and Biotechnology, 2014, 5, 209-215 ABB http://dx.doi.org/10.4236/abb.2014.53027 Published Online February 2014 (http://www.scirp.org/journal/abb/) Genetic characterization of far eastern species of the genus Crepidostomum (Trematoda: Allocreadiidae) by means of 28S ribosomal DNA sequences Dmitry M. Atopkin1,2*, Marina B. Shedko1 1Lab. Parasitology, Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia 2Far Eastern Federal University, Vladivostok, Russia Email: *[email protected] Received 14 November 2013; revised 13 January 2014; accepted 26 January 2014 Copyright © 2014 Dmitry M. Atopkin, Marina B. Shedko. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In accordance of the Creative Commons Attribution License all Copyrights © 2014 are reserved for SCIRP and the owner of the intellectual property Dmitry M. Atopkin, Marina B. Shedko. All Copyright © 2014 are guarded by law and by SCIRP as a guardian. ABSTRACT Trematoda; Digenea; Phylognetic Relationships Genetic divergence and phylogenetic relationships of four species of the genus Crepidostomum Braun, 1900 1. INTRODUCTION sensu Caira, Bogĕa (2005) were revealed using partial Species of the Allocreadiidae are important components sequences of 28S ribosomal RNA gene. Genetic di- of the parasite fauna of the freshwater fishes and their vergence between C. cf. farionis (Muller, 1784) and C. systematics have not been clarified yet. The membership nemachilus Krotov, 1959 was 3.1%, which corres- of these trematodes to either family Allocreadiidae Stos- ponds to the mean value of interspecific divergence sich, 1903 or family Bunoderidae Nicoll, 1914 has been between Crepidostomum species.
    [Show full text]
  • Curriculum Vitae
    1 CURRICULUM VITAE DR. GERARDO PÉREZ PONCE DE LEÓN Mayo, 2019 2 1.- DATOS PERSONALES Nombre: GERARDO PEREZ PONCE DE LEON. Fecha y Lugar de Nacimiento: 18 de Febrero de 1963. México, D.F. Teléfono: Oficina: 56229131 Correo Electrónico: [email protected] Estado Civil: Casado 2.- NOMBRAMIENTO ACTUAL: INVESTIGADOR TITULAR "C" de T.C. (desde el año 2002) 3.- SISTEMA NACIONAL DE INVESTIGADORES: INV. NACIONAL NIV. III (Julio 2004 - 30 Diciembre del 2024). Miembro Regular de la Academia Mexicana de Ciencias. Noviembre 2004- OTRAS DISTINCIONES: - Premio Nacional de Investigación en Alimentos en el Area de Aspectos de Frontera en Biología Animal Día Mundial de la Alimentación. 16 de Octubre de 1987. Mención Honorífica. - Medalla “GABINO BARREDA” como reconocimiento a los estudios de Doctorado en Ciencias (Biología) en la Facultad de Ciencias, UNAM. Noviembre 14, 1996. - Premio de Investigación en Ciencias Naturales. Academia Mexicana de Ciencias. 2002. - Henry Baldwin Ward Medal. American Society of Parasitologists. 2005 - Clark P. Reid Mentor Award. American Society of Parasitologists. 2014 4.- ANTIGÜEDAD EN LA UNAM: 34 Años (Diciembre 9, 1984- 3 Programa Estímulos a Productividad y Rendimiento Académico PRIDE D 2002-2022 5.- FORMACIÓN ACADÉMICA 5.1 Licenciatura: Biología. Facultad de Ciencias, UNAM.1982-1986. Obtención: 9 de diciembre de 1986. 5.2 Maestría en Ciencias (Biología).Facultad de Ciencias, UNAM;1986-1988. Obtención: 25 de abril de 1989 5.3 Doctorado en Ciencias (Biología). Fac.de Ciencias,UNAM;1989-1992. Obtención: 24 de septiembre, 1992 5.4 Posdoctorado: Universidad de Toronto, Departamento de Zoología. Canadá. Proyecto: Taxonomía y Relaciones Filogenéticas de la Familia Pronocephalidae (Digenea).
    [Show full text]
  • Serotonin and Neuropeptide Fmrfamide in the Attachment Organs of Trematodes
    ©2018 Institute of Parasitology, SAS, Košice DOI 10.2478/helm-2018-0022 HELMINTHOLOGIA, 55, 3: 185 – 194, 2018 Serotonin and neuropeptide FMRFamide in the attachment organs of trematodes N. B. TERENINA1*, N. D. KRESHCHENKO2, N. B. MOCHALOVA1, S. O. MOVSESYAN1 1А.N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, Center of Parasitology, 119071 Moscow, Leninsky pr. 33, Russia, *E-mail: [email protected]; 2Institute of Cell Biophysics of Russian Academy of Sciences, Moscow Region, Pushchino, Institutskaya 3, Russia Article info Summary Received April 2, 2018 The serotoninergic and FMRFamidergic nervous system of the attachment organs of trematodes Accepted June 5, 2018 were examined using immunocytochemical techniques and confocal scanning laser microscopy. Adult trematodes from eight families as well as cercariae and metacercariae from ten families were studied. TRITC-conjugated phalloidin was used to stain the muscle fi bres. The serotonin- and FMR- Famide-immunoreactive (IR) nerve cells and fi bres were revealed to be near the muscle fi bres of the oral and ventral suckers of the trematodes and their larvae. The results indicate the important role of neurotransmitters, serotonin and neuropeptide FMRFamide in the regulation of muscle activity in the attachment organs of trematodes and can be considered in perspective for the development of new anthelmintic drugs, which can interrupt the function of the attachment organs of the parasites. Keywords: Trematodes; attachment organs; neurotransmitters; serotonin; FMRFamide; nervous system Introduction riculture. That is why studying this animal group is important, not only for solving fundamental medical problems, but also for having The parasitic fl atworms, trematodes have well-developed adhe- great practical signifi cance.
    [Show full text]
  • Neotropical Vol. 9
    ISSN Versión impresa 2218-6425 ISSN Versión Electrónica 1995-1043 REVIEW ARTICLE / ARTÍCULO DE REVISIÓN CHROMOSOMES AND CYTOGENETICS OF HELMINTHS (TURBELLARIA, TREMATODA, CESTODA, NEMATODA AND ACANTHOCEPHALA) CROMOSOMAS Y CITOGENÉTICA DE HELMINTOS (TURBELLARIA, TREMATODA, CESTODA, NEMATODA Y ACANTHOCEPHALA) Tanveer A. Sofi1, Fayaz Ahmad1, Bashir A. Sheikh1, Omer Mohi Ud Din Sofi2 & Khalid M. Fazili3 1Post Graduate Department of Zoology, University of Kashmir, Srinagar, Kashmir, 1900 06, India. 2SK University of Agricultural Sciences and Technology, Shuhama, Aluestang Srinagar, 1900 06, India. 3Post Graduate Department of Bitechnology, University of Kashmir, Srinagar, Kashmir, 1900 06 Ph. No. India. 09797127214. [email protected] Neotropical Helminthology, 2015, 9(1), jan-jun: 113-162. ABSTRACT We review the literature from 1886 to 2014 and the current status of knowledge of the chromosomes and cytogenetics of all species of Turbellaria, Trematoda, Cestoda, Nematoda, and Acanthocephala. Karyological data are discussed and tabulated for 614 species: 115 species of Turbellaria, 278 species of Trematoda, 117 species of Cestoda, 85 species of Nematoda and 19 species of Acanthocephala. Turbellarians are not parasitic except for a few possible exceptions and they show a gradual reduction of the basic number of chromosomes. Trematodes are numerous which points towards the continued efforts in this field of research. Data on chromosomes are lacking for acetabulate cestodes of the orders: Litobothriidea, Lecanicephalidea, Cathetocephalidea, Rhinebothriidea and Tetrabothriidea. Keywords: chromosomes – cytogenetics - Acanthocephala- Cestoda- - Nematoda- Trematoda- Turbellaria. RESUMEN En este artículo revisamos la literatura desde 1886 hasta 2014 y el estado actual del conocimiento de los cromosomas y la citogenética de todas las especies de las familias de turbellaria, trematoda, cestoda, nematoda y acanthocephala.
    [Show full text]
  • Guide to the Parasites of Fishes of Canada
    Canadian Special Publication of Fisheries and Aquatic Sciences 124 Guide to the Parasites of Fishes of Canada Edited by L Margolis and Z Kabata 11111111illyellfill Part IV Trematoda David L Gibson m Department ori Fisheries & Orean's Library rAu°Anur 22 1996 Ministere cles Perches et Oceans des OTTAWA c 3 1 ( LF cJ GUIDE TO THE PARASITES OF FISHES OF CANADA PART IV NRC Monograph Publishing Program R.H Haynes, OC, FRSC (York University): Editor, Monograph Publishing Program Editorial Board: W.G.E. Caldwell, FRSC (University of Western Ontario); P.B. Cavers (University of Western Ontario); G. Herzberg, CC, FRS, FRSC (NRC, Steacie Institute of Molecular Sciences); K.U. IngoId, OC, FRS, FRSC, (NRC, Steacie Institute of Molecular Sciences); M. Lecours (Université Laval); L.P. Milligan, FRSC (University of Guelph); G.G.E. Scudder, FRSC (University of British Columbia); E.W. Taylor, FRS (University of Chicago); B.P. Dan- cik, Editor-in-Chief, NRC Research Journals and Monographs (University of Alberta) Publishing Office: M. Montgomery, Director General, CISTI; A. Holmes, Director, Publishing Directorate; G.J. Neville, Head, Monograph Publishing Program; E.M. Kidd, Publication Officer. Publication Proposals: Proposals for the NRC Monograph Publishing Program should be sent to Gerald J. Neville, Head, Monograph Publishing Program, National Research Council of Canada, NRC Research Press, 1200 Montreal Road, Building M-55, Ottawa, ON K 1 A 0R6, Canada. Telephone: (613) 993-1513; fax: (613) 952-7656; e-mail: gerry.nevi lie@ nrc.ca . © National Research Council of Canada 1996 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior written permission of the National Research Council of Canada, Ottawa, Ontario KlA 0R6, Canada.
    [Show full text]
  • A1078e02.Pdf
    5 PARASITE-HOST LIST 7 KINGDOM PROTISTA3 FAMILY BODONIDAE SUBKINGDOM PROTOZOA Ichthyobodo necator (F,B,M) PHYLUM MASTIGOPHORA (Henneguy, 1884) Pinto, 1928 Syn.: Costia necatrix Henneguy, 1884 CLASS KINETOPLASTIDEA Location: gills, skin Hosts:Blicca bjoerkna ORDER KINETOPLASTIDA Carassius carassius Dist.: Lake Rāznas, Daugava River SUBORDER TRYPANOSOMATINA Records: Shulman 1949; Kirjusina & Vismanis 2004 FAMILY TRYPANOSOMATIDAE Remarks: A dangerous ectoparasite for practically all fish, Ichthyobodo causes mortalities mainly of young fish and those Trypanosoma carassii (F) with lowered resistance (see Lom and Mitrophanow, 1883 Dyková 1992). Syn.: Trypanosoma danilewskyi Laveran and Mesnil, 1904 Includes: T. gracilis of Kirjusina and CLASS DIPLOMONADEA Vismanis, 2004 Location: blood ORDER DIPLOMONADIDA Host: Cyprinus carpio carpio Dist.: Latvia (ponds) FAMILY HEXAMITIDAE Records: Vismanis & Peslak 1963; Vismanis 1964; Kirjusina & Vismanis 2004 Remarks: This trypanosome is a pathogen of Hexamita salmonis (Moore, 1923) (F,B) juvenile common carp. The leeches Piscicola Wenyon, 1926 geometra and Hemiclepsis marginata are Syn.: Hexamita truttae (Schmidt, 1920) reported to be vectors (see Lom and Dyková Octomitus truttae Schmidt, 1920 1992). Location: gall bladder, intestine The synonymy follows Lom and Hosts: Lota lota (1,4) Dyková (1992). Oncorhynchus mykiss (2,3,4) Salmo salar (2,4) Dist.: Lake Rāznas, Kegums Water Reservoir, Trypanosoma granulosum (F) Daugava River Laveran and Mesnil, 1909 Records: 1. Shulman 1949 (Lake Rāznas, Location: blood Daugava River); 2. Vismanis, Kuznetsova, Host: Anguilla anguilla & Rakitsky 1983 (hatchery); 3. Lullu et al. Dist.: Lake Usmas; Venta River; Gulf of Riga 1989 (tanks); 4. Kirjusina & Vismanis 2004 Records: Kirjusina & Vismanis 2000 (Lake (Lake Rāznas, Kegums Water Reservoir, Usmas, Venta River, Gulf of Riga), 2004 tanks) (Lake Usmas) Remarks: The pathogenicity of this flagellate Remarks: The leeches Piscicola geometra and is not clear.
    [Show full text]
  • T. C. Uludağ Üniversitesi Fen Bilimleri Enstitüsü Türkiye Endemiği Siraz Baliği Capoeta Baliki (Turan, Kotte
    T. C. ULUDAĞ ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ TÜRKİYE ENDEMİĞİ SİRAZ BALIĞI CAPOETA BALİKİ (TURAN, KOTTELAT, EKMEKÇİ & İMAMOĞLU, 2006) (CYPRINIFORMES: CYPRINIDAE) 'NİN HELMİNT FAUNASI MCEBO EMMANUEL MNISI TÜRKİYE ENDEMİĞİ SİRAZ BALIĞI CAPOETA BALİKİ (TURAN, KOTTELAT, EKMEKÇİ & İMAMOĞLU, 2006) (CYPRINIFORMES: CYPRINIDAE) 'NİN HELMİNT FAUNASI MCEBO EMMANUEL MNISI T. C. ULUDAĞ ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ TÜRKİYE ENDEMİĞİ SİRAZ BALIĞI CAPOETA BALIKİ (TURAN, KOTTELAT, EKMEKÇİ & İMAMOĞLU, 2006) (CYPRINIFORMES: CYPRINIDAE) 'NİN HELMİNT FAUNASI MCEBO EMMANUEL MNİSİ Prof. Dr. Hikmet S. YILDIRIMHAN (Danışman) YÜKSEK LİSANS TEZİ BİYOLOJİ ANABİLİM DALI BURSA- 2017 Her hakkı saklıdır ÖZET Yüksek Lisans Tezi TÜRKİYE ENDEMİĞİ SİRAZ BALIĞI, CAPOETA BALIKİ (TURAN, KOTTELAT, EKMEKÇİ & İMAMOĞLU, 2006) (CYPRINIFORMES: CYPRINIDAE) 'NİN HELMİNT FAUNASI MCEBO EMMANUEL MNİSİ Uludağ Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Danışman: Prof. Dr. Hikmet Sami YILDIRIMHAN Şubat, Mart ve Nisan 2017 Ayları sırasında, 65 Siraz Balığı Capoeta baliki, Eskişehir'de Seydi Nehri'nden helmint parazitleri incelenmek üzere toplantı. Bu balıklardan 24’ünde helmint parazit raslanmıştır ve toplam 65 parazit gözlenmiştir. Kaydedilen bu parazitler 1 Digeanea; Allocreadium isoporomu ve 3 nematod türü; Contracaecum sp, Eustrongylides excisus ve Rabdochona denutata'dan oluşmaktadır. Allocreadium isoporom 12 balıkta (% 18.46) bulunmuştur. Balıkların% 12.31'ini oluşturan sekiz balık, Contracaecum sp., Eustrongylides excisus ve Rabdochona denutata ile enfekte
    [Show full text]
  • Checklist of the Parasites of Fishes of Viet Nam
    FAO Checklist of the parasites FISHERIES TECHNICAL of fishes of Viet Nam PAPER 369/2 by J. Richard Arthur Barriere, British Columbia Canada and Bui Quang Te Research Institute for Aquaculture No. 1 Din Bang, Tien Son, Bac Ninh Viet Nam FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS Rome, 2006 The designations employed and the presentation of material in this information product do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal or development status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. ISBN 978-92-5-105635-6 All rights reserved. Reproduction and dissemination of material in this in- formation product for educational or other non-commercial purposes are authorized without any prior written permission from the copyright holders provided the source is fully acknowledged. Reproduction of material in this information product for resale or other commercial purposes is prohibited without written permission of the copyright holders. Applications for such permission should be addressed to the Chief, Electronic Publishing Policy and Support Branch, Information Division, FAO, Viale delle Terme di Caracalla, 00153 Rome, Italy or by e-mail to [email protected] © FAO 2006 iii PREPARATION OF THIS DOCUMENT This checklist is part of the continuing effort of the Food and Agriculture Organization of the United Nations to address the need for information on the occurrence of diseases and pathogens of aquatic animals in the Asia-Pacific Region. Two previous checklists, published as FAO Fisheries Technical Papers Nos.
    [Show full text]
  • Ms, and Data from the Freshwater Environment Are 57 Scarce
    1 Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake 2 3 Miroslava Soldánová a,†, Simona Georgieva a,†, Jana Roháčová a,b, Rune Knudsen c, Jesper A. 4 Kuhn c, Eirik H. Henriksen c, Anna Siwertsson c, Jenny C. Shaw d, Armand M. Kuris d, Per-Arne 5 Amundsen c, Tomáš Scholz a,b, Kevin D. Lafferty e, Aneta Kostadinova a,* 6 7 a Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05 8 České Budějovice, Czech Republic 9 b Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, 10 Czech Republic 11 c Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, 12 UiT The Arctic University of Norway, N9037 Tromsø, Norway 13 d Department of Ecology, Evolution and Marine Biology and Marine Science Institute, University 14 of California, Santa Barbara, California 93106 USA 15 e United States Geological Survey, Western Ecological Research Center c/o Marine Science 16 Institute, University of California, Santa Barbara, California 93106 USA 17 18 † Equal contributors 19 * Corresponding author. Tel.: +420-38-777 5933; fax: +420-38-5310388. 20 E-mail address: [email protected]; [email protected] 21 22 23 24 25 26 Note: Supplementary data associated with this article. 27 28 29 International Journal for Parasitology 47 (2017) 327–345 30 http://dx.doi.org/10.1016/j.ijpara.2016.12.008 1 31 ABSTRACT 32 To identify trematode diversity and life-cycles in the sub-Arctic lake Takvatn, we characterised 33 120 trematode isolates from mollusc first intermediate hosts and metacercariae from second 34 intermediate host fishes and invertebrates using molecular techniques.
    [Show full text]
  • A Database on Parasites of Freshwater and Brackish Fish in the United Kingdom
    Aquatic Parasite Information – a Database on Parasites of Freshwater and Brackish Fish in the United Kingdom A thesis submitted to Kingston University London for the Degree of Doctor of Philosophy by Bernice BREWSTER BSc (Hons.) London June 2016 Declaration This thesis is being submitted in partial fulfilment of the requirements of Kingston University, London for the award of Doctor of Philosophy. I confirm that all work included in this thesis was undertaken by myself and is the result of my own research and all sources of information have been acknowledged. Bernice Brewster 2nd June 2016 I Abstract A checklist of parasites of freshwater fish in the UK is an important source of information concerning hosts and their distribution for all aspects of scientific research. An interactive, electronic, web-based database, Aquatic Parasite Information has been designed, incorporating all freshwater and brackish species of fish, parasites, taxonomy, synonyms, authors and associated hosts, together with records for their distribution. One of the key features of Aquatic Parasite Information is this checklist can be updated. Interrogation of Aquatic Parasite Information has revealed that some parasites of freshwater and brackish species of fish, such as the unicellular groups or those metazoans that are difficult to identify using morphological characters, are under reported. Aquatic Parasite Information identified the monogenean family Dactylogyridae and the cestodes infecting UK freshwater fish as under-represented groups, owing to the difficulties identifying them morphologically. Both the Dactylogyridae and cestodes have implications for pathology, outbreaks of disease and morbidity in freshwater fish in the UK, therefore accurate identification is critical. Studies were undertaken using both standard morphological techniques of histology and molecular techniques to identify dactylogyrid species and tapeworms commonly found parasitizing fish in the UK.
    [Show full text]