Journal of General Virology (2014), 95, 1352–1365 DOI 10.1099/vir.0.064436-0 A novel papillomavirus in Ade´lie penguin (Pygoscelis adeliae) faeces sampled at the Cape Crozier colony, Antarctica Arvind Varsani,1,2,3 Simona Kraberger,1 Scott Jennings,4 Elizabeth L. Porzig,5 Laurel Julian,1 Melanie Massaro,6 Annie Pollard,5 Grant Ballard7 and David G. Ainley5 Correspondence 1School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Arvind Varsani Private Bag 4800, Christchurch 8140, New Zealand
[email protected] 2Department of Plant Pathology and Emerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USA 3Electron Microscope Unit, Division of Medical Biochemistry, Department of Clinical Laboratory Sciences, University of Cape Town, Observatory, 7700, South Africa 4Department of Fisheries and Wildlife, Oregon Cooperative Fish and Wildlife Research Unit, US Geological Survey, Oregon State University, Corvallis, OR, USA 5HT Harvey and Associates, Los Gatos, CA 95032, USA 6School of Environmental Sciences, Charles Sturt University, Albury, NSW 2640, Australia 7Point Blue Conservation Science, Petaluma, CA 94954, USA Papillomaviruses are epitheliotropic viruses that have circular dsDNA genomes encapsidated in non-enveloped virions. They have been found to infect a variety of mammals, reptiles and birds, but so far they have not been found in amphibians. Using a next-generation sequencing de novo assembly contig-informed recovery, we cloned and Sanger sequenced the complete genome of a novel papillomavirus from the faecal matter of Ade´lie penguins (Pygoscelis adeliae) nesting on Ross Island, Antarctica. The genome had all the usual features of a papillomavirus and an E9 ORF encoding a protein of unknown function that is found in all avian papillomaviruses to date.