DOCSLIB.ORG

(12) United States Patent (10) Patent No.: US 8,501,463 B2 Cox Et Al

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(12) United States Patent (10) Patent No.: US 8,501,463 B2 Cox Et Al USOO85O1463B2 (12) United States Patent (10) Patent No.: US 8,501,463 B2 Cox et al. (45) Date of Patent: Aug. 6, 2013 (54) ANAEROBC PRODUCTION OF HYDROGEN (56) References Cited AND OTHER CHEMICAL PRODUCTS U.S. PATENT DOCUMENTS (75) Inventors: Marion E. Cox, Morgan Hill, CA (US); 5,350,685 A 9/1994 Taguchi et al. Laura M. Nondorf, Morgan Hill, CA 5,464,539 A 11/1995 Ueno et al. 6,090,266 A 7/2000 Roychowdhury (US); Steven M. Cox, Morgan Hill, CA 6,251,643 B1 6/2001 Hansen et al. (US) 6,299,774 B1 * 10/2001 Ainsworth et al. ........... 210,603 6,342,378 B1 1/2002 Zhang et al. (73) Assignee: Anaerobe Systems, Morgan Hill, CA 6,569,332 B2 * 5/2003 Ainsworth et al. ........... 210,603 2004/0050778 A1 3/2004 Noike et al. (US) 2004/O115782 A1 6/2004 Paterek (*) Notice: Subject to any disclaimer, the term of this FOREIGN PATENT DOCUMENTS patent is extended or adjusted under 35 WO WO-2006-119052 A2 11/2006 U.S.C. 154(b) by 1347 days. OTHER PUBLICATIONS (21) Appl. No.: 11/912,881 Liu et al., 2004. Effects of Culture and Medium Conditions on Hydro gen Production from Starch Using Anaerobic Bacteria. Journal of (22) PCT Fled: Apr. 27, 2006 Bioscience and Bioengineering, vol. 98, No. 4, pp. 251-256.* Zhang et al., Distributed Computer Control of Penicillin Fermenta (86) PCT NO.: PCT/US2OO6/O16332 tion Industrial Production. Proceedings of the IEEE International Conference on Industrial Technology, 1996, pp. 52-56.* S371 (c)(1), New Brunswick, an eppenforf Company, pp. 1-3. http://www.nbsc. (2), (4) Date: Jun. 23, 2008 com/BioFlo-415-SIP-Fermentor.aspx Printed Mar. 26, 2012.* Prell et al. 2000. Lower layer control of the CSTR, a basic part of the bioreactor control system. 27th International Conference of Slovak (87) PCT Pub. No.: WO2O06/119052 Society of Chemical Engineering, Tatranske Matliare, Slovakia, PCT Pub. Date: Nov. 9, 2006 Proc. p. 30, Lecture No. R16.* Claassen, P.A.M. et al., “Utilisation of biomass for the supply of energy carriers.” Appl. Microbiol. Biotechnol. 52:741-755 (1999). (65) Prior Publication Data Gorman, J., “Hydrogen: The Next Generation, Cleaning up productionof a future fuel.” 8 pages retrieved from Internet on Oct. US 2008/0311640 A1 Dec. 18, 2008 27, 2004 http://www.sciencenews.org/articles/2w0021012/bob11. asp. Henley, M. et al., “Microbial Hydrogen Production.” 3 pages Related U.S. Application Data retrieved from Internet on Oct. 27, 2004 http://www.afrlhorizons. com/Briefs, Jun03/MLO227.html. (60) Provisional application No. 60/678,101, filed on May Levin, D.B. et al., “Biohydrogen production: prospects and limita 3, 2005, provisional application No. 60/677,856, filed tions to practical application.” Intl. J. Hydrogen Energy 29:173-185 (2004). on May 3, 2005, provisional application No. Logan, B. et al., "Biological Hydrogen Production as a Sustainable 60/678,077, filed on May 3, 2005, provisional GreenTechnology for Pollution Prevention.” 2 pages retrieved from application No. 60/678,100, filed on May 3, 2005, Internet on Oct. 27, 2004 http://www.engr/psu.edu/h2e/Pub/Logan provisional application No. 60/678,098, filed on May etal 1.htm. Logan, B. et al., “Biological Hydrogen Production in a Bioreactor” 1 3, 2005, provisional application No. 60/677,998, filed page retrieved from Internet on Oct. 27, 2004 http://www.engr/psu. on May 3, 2005. edu/h2e/Pub/Logan etal 1.htm. (51) Int. C. (Continued) CI2M I/07 (2006.01) CI2M I/2 (2006.01) Primary Examiner—Jon PWeber CI2P3/00 (2006.01) Assistant Examiner — Kailash C Srivastava CI2P I/04 (2006.01) (74) Attorney, Agent, or Firm — Wilson, Sonsini, Goodrich CI2N L/20 (2006.01) & Rosati CI2M I/36 (2006.01) CI2M 1/38 (2006.01) (57) ABSTRACT CI2M 1/107 (2006.01) Described herein are methods for producing chemical prod (52) U.S. C. ucts by anaerobically fermenting a particular biomass using USPC ..................... 435/303.2; 435/168; 435/252.1; anaerobic bacteria. Such chemical products includehydrogen 435/286.1; 435/290.4 and other gases, acetic acid and other volatile organic acids, (58) Field of Classification Search Solvents, Solids, and salts of Volatile organic acids. USPC .............. 435/168,252.1, 286.1, 290.4, 303.2 See application file for complete search history. 5 Claims, 18 Drawing Sheets US 8,501.463 B2 Page 2 OTHER PUBLICATIONS Hussy et al., “Continuous Fermentative Hydrogen Production from a Wheat Starch Co-Product by Mixed Microfloara.” Biotech. Bioeng. Logan, B. et al., “Biological Hydrogen Production Measured in 84(6):619-626 (2003). Batch Anaerobic Respirometers.” Environ. Sci. Technol. 36:2530 Lay, J.J. et al., “Influence of chemical nature of organic wastes on 2535 (2002). Oh, S.E. et al., “The Relative Effectiveness of pH Control and Heat their conversion to hydrogen by heat-shock digested sludge.” Intl. J. Treatment for Enhancing Biohydrogen Gas Production.” Environ. Hydrogen Energy 28:1361-1367 (2003). Sci. Technol. 37:5186-5190 (2003). Taguchi et al., “Direct conversion of ceulolosic materials to hydro Raskin, S.S. et al., “Hydrogen Production by Anaerobic Microbial gen by Clostridium sp. Strain No. 2. Enzyme and Microbial Tech. Communities Exposed to Repeated Heat Treatments.” Proceedings 17: 147-150 (1995). of the 2002 U.S. DOE Hydrogen Program Review, NREL/CP-610 Yokol, H. et al., “Microbial production of hydrogen from starch 32405, 17 pages. manufacturing wastes.” Biomass and Bioenergy 22:389-395 (2002). Regan, J. et al., “Genetic Engineering of Chlostridium PCT/US06/16332 Search Report Dated Sep. 12, 2008. acetobutylicum for Enhanced Production of Hydrogen Gas, 1 page retrieved from Internet on Oct. 27, 2004 http://www.engrpsu.edu/ Abraham et al., "Commissioning and Re-Design of a Class A Ther h2e/Pub/Regan etal 1.htm. mal Hydrolysis Facility for Pre-Treatment of Primary and Secondary Ren, N.O. et al., “Hydrogen Production from Molasses by Anaerobic Sludge Prior to Anaerobic Digestion.” 76' Annual Water Environ Activated Sludge in Pilot-Scale Bioreactor.” Poster Presentation 2-27 ment Federation Technical Exposition and Conference, from 26' Symposium on Biotechnology for Fuels and Chemicals, XP002660698, pp. 1-14 (2003) Retrieved from the Internet: May 9-12, 2004, Chattanooga, TN USA, retrieved from Internet on URL:http://www.cambi.no/photoalbum/view2. Dec. 17, 2004, http://www.ct.ornil.gov/symposium/index files/2ab P3NpemU9b3JnJmlkPTIyMDAZMCZOeXB1PTE *abstract *p. 2, stracts/2 27.htm. last paragraph-p. 3, last paragraph. Taguchi, F. et al., “Continuous Hydrogen Production by Clostridium Wang et al., “Producing Hydrogen from Wastewater Sludge by sp. Strain No. 2 from Cellulose Harolysate in an Aqueous Two-Phase Clostridium Bifermentans,” Journal of Biotechnology, vol. 102, No. System.” J. Fermentation and Bioengineering 82(1):80-83 (1996). 1, pp. 83-92 (2003). Van Ginkel, S. et al., “Role of Initial Sucrose and pH Levels on EP06751822.5 Extended European Search Report mailed Oct. 18, Natural, Hydrogen-Producing, Anaerobe Germination.” Proceedings 2011. of the 2001 DOE Hydrogen Program Review, NREL/CP-570 3053.15 pages. * cited by examiner U.S. Patent Aug. 6, 2013 Sheet 2 of 18 US 8,501.463 B2 + z?un61-I SSeuJ01E U.S. Patent Aug. 6, 2013 Sheet 3 of 18 US 8,501,463 B2 €3.Infi!-- wrwryorms Vancornycfnsuscesceptible. -- U.S. Patent Aug. 6, 2013 Sheet 4 of 18 US 8,501.463 B2 #7eun61-I uwww.sax ter-r mini-in. al-rial waterinerrassisternaror U.S. Patent Aug. 6, 2013 Sheet 5 of 18 US 8,501.463 B2 ----****.xxx****** ?un61-IG U.S. Patent Aug. 6, 2013 Sheet 9 of 18 US 8,501.463 B2 l U.S. Patent Aug. 6, 2013 Sheet 11 of 18 US 8,501.463 B2 ??eun61-I U.S. Patent Aug. 6, 2013 Sheet 12 of 18 US 8,501.463 B2 U.S. Patent Aug. 6, 2013 Sheet 13 of 18 US 8,501.463 B2 €),bun61-I SSeluOIRJO |d??S SSeluO?g30 Zd??S U.S. Patent Aug. 6, 2013 Sheet 14 of 18 US 8,501.463 B2 | U.S. Patent Aug. 6, 2013 Sheet 16 of 18 US 8,501.463 B2 SSEuIO/g U.S. Patent Aug. 6, 2013 Sheet 17 of 18 US 8,501.463 B2 L?,eun61-I No.sae quoudinbº U.S. Patent Aug. 6, 2013 Sheet 18 of 18 US 8,501.463 B2 ?un61-I8), US 8,501,463 B2 1. 2 ANAEROBC PRODUCTION OF HYDROGEN into chemical products. In another aspect are assemblages AND OTHER CHEMICAL PRODUCTS which comprise sources of steam; turbines; and digesters. In one aspect is the use of bacteria for the production of CROSS REFERENCE hydrogen and other chemical products. In another aspect biomass prepared for use in hydrogen production has been This application claims the benefit of U.S. provisional sterilized, deoxygenated, concentrated, detoxificated, and/or application Ser Nos. 60/678,101, filed May 3, 2005; 60/677, pre-digested while optimal bacterial strains for anaerobic 856, filed May 3, 2005; 60/678,077, filed May 3, 2005; fermentation of the biomass have been selected by means of 60/678,100, filed May 3, 2005; 60/678,098, filed May 3, a Knowledge Management System, and selected and isolated 2005; and 60/677,998, filed May 3, 2005, all of which are 10 using an isolation/enrichment system. incorporated by reference in their entirety. Bacterial Strains In further or alternative embodiments of all aspects FIELD OF THE INVENTION described herein using bacterial strains to anaerobically fer ment biomass and obtain chemical products therefrom, the The methods and compositions described herein are 15 bacterial strains are substantially purified anaerobic bacteria directed to the production of hydrogen and other chemical and such substantially purified bacterial strains are selected to products via the anaerobic bacterial fermentation of biomass, anaerobically ferment the biomass, and in still further or and the production of hydrogen and other chemical products alternative embodiments of all aspects, the Substantially puri via bacterial conversion of products obtained from anaerobic fied bacterial strains are selected from the group consisting of fermentation.
Load more

Recommended publications
  • The Microbial Ecology of Sulfur Transformations in Oyster Pond, Woods Hole, Massachusetts
    University of New Hampshire University of New Hampshire Scholars' Repository Doctoral Dissertations Student Scholarship Spring 1970 THE MICROBIAL ECOLOGY OF SULFUR TRANSFORMATIONS IN OYSTER POND, WOODS HOLE, MASSACHUSETTS FRANK W. BARVENIK Follow this and additional works at: https://scholars.unh.edu/dissertation Recommended Citation BARVENIK, FRANK W., "THE MICROBIAL ECOLOGY OF SULFUR TRANSFORMATIONS IN OYSTER POND, WOODS HOLE, MASSACHUSETTS" (1970). Doctoral Dissertations. 933. https://scholars.unh.edu/dissertation/933 This Dissertation is brought to you for free and open access by the Student Scholarship at University of New Hampshire Scholars' Repository. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of University of New Hampshire Scholars' Repository. For more information, please contact [email protected]. 71-5863 BARVENIK, Frank W., 1943- | THE MICROBIAL ECOLOGY OF SULFUR TRANSFORMATIONS I IN OYSTER POND, WOODS HOLE, MASSACHUSETTS. f i University of New Hampshire, Ph.D., 1970 f Microbiology University Microfilms, Inc., Ann Arbor, Michigan THIS DISSERTATION HAS BEEN MICROFILMED EXACTLY AS RECEIVED THE MICROBIAL ECOLOGY OF SULFUR TRANSFORMATIONS IN OYSTER POND, WOODS HOLE, MASSACHUSETTS by FRANK W. BARVENIK B.A., University of Connecticut, 1965 A THESIS Submitted to the University of New Hampshire In Partial Fulfillment of The Requirements for the Degree of Doctor of Philosophy Graduate School Department of Microbiology June, 1970 This thesis has been examined and approved. C. __ _ Thesisis director, #£len2alen E. Jones, Prof. of Microbiology William R. Chesbro, Prof. of Microbiology ______ jawrence W. Slanetz, Prof. of M igroipiology IjHtVX 7 ~ _____ ___ _________________ Henri E. Gaudettfy, Asso. Prcff. of Geiology Sanuiel C.
    [Show full text]
  • Arxiv:2105.11503V2 [Physics.Bio-Ph] 26 May 2021 3.1 Geometry and Swimming Speeds of the Cells
    The Bank Of Swimming Organisms at the Micron Scale (BOSO-Micro) Marcos F. Velho Rodrigues1, Maciej Lisicki2, Eric Lauga1,* 1 Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge CB3 0WA, United Kingdom. 2 Faculty of Physics, University of Warsaw, Warsaw, Poland. *Email: [email protected] Abstract Unicellular microscopic organisms living in aqueous environments outnumber all other creatures on Earth. A large proportion of them are able to self-propel in fluids with a vast diversity of swimming gaits and motility patterns. In this paper we present a biophysical survey of the available experimental data produced to date on the characteristics of motile behaviour in unicellular microswimmers. We assemble from the available literature empirical data on the motility of four broad categories of organisms: bacteria (and archaea), flagellated eukaryotes, spermatozoa and ciliates. Whenever possible, we gather the following biological, morphological, kinematic and dynamical parameters: species, geometry and size of the organisms, swimming speeds, actuation frequencies, actuation amplitudes, number of flagella and properties of the surrounding fluid. We then organise the data using the established fluid mechanics principles for propulsion at low Reynolds number. Specifically, we use theoretical biophysical models for the locomotion of cells within the same taxonomic groups of organisms as a means of rationalising the raw material we have assembled, while demonstrating the variability for organisms of different species within the same group. The material gathered in our work is an attempt to summarise the available experimental data in the field, providing a convenient and practical reference point for future studies. Contents 1 Introduction 2 2 Methods 4 2.1 Propulsion at low Reynolds number .
    [Show full text]
  • Int J Syst Evol Microbiol 67 1
    Author version : International Journal of Systematic and Evolutionary Microbiology, vol.67(6); 2017; 1949-1956 Imhoffiella gen. nov.. a marine phototrophic member of family Chromatiaceae including the description of Imhoffiella purpurea sp. nov. and the reclassification of Thiorhodococcus bheemlicus Anil Kumar et al. 2007 as Imhoffiella bheemlica comb. nov. Nupur1, Mohit Kumar Saini1, Pradeep Kumar Singh1, Suresh Korpole1, Naga Radha Srinivas Tanuku2, Shinichi Takaichi3 and Anil Kumar Pinnaka1* 1Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh – 160 036, INDIA 2CSIR-National Institute of Oceanography, Regional Centre, 176, Lawsons Bay Colony, Visakhapatnam-530017, INDIA 3Nippon Medical School, Department of Biology, Kyonan-cho, Musashino 180-0023, Japan Address for correspondence* Dr. P. Anil Kumar Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology (CSIR), Sector 39A, Chandigarh – 160 036, INDIA Email: [email protected] Telephone: 00-91-172-6665170 Running title Imhoffiella purpurea sp. nov. Subject category New taxa (Gammaproteobacteria) The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain AK35T is HF562219. A coccoid-shaped phototrophic purple sulfur bacterium was isolated from a coastal surface water sample collected from Visakhapatnam, India. Strain AK35T was Gram-negative, motile, purple colored, containing bacteriochlorophyll a and the carotenoid rhodopinal as major photosynthetic pigments. Strain AK35T was able to grow photoheterotrophically and could utilize a number of organic substrates. It was unable to grow photoautotrophically. Strain AK35T was able to utilize sulfide and thiosulfate as electron donors. The main fatty acids present were identified as C16:0, C18:1 T 7c and C16:1 7c and/or iso-C15:0 2OH (Summed feature 3) were identified.
    [Show full text]
  • Coupled Reductive and Oxidative Sulfur Cycling in the Phototrophic Plate of a Meromictic Lake T
    Geobiology (2014), 12, 451–468 DOI: 10.1111/gbi.12092 Coupled reductive and oxidative sulfur cycling in the phototrophic plate of a meromictic lake T. L. HAMILTON,1 R. J. BOVEE,2 V. THIEL,3 S. R. SATTIN,2 W. MOHR,2 I. SCHAPERDOTH,1 K. VOGL,3 W. P. GILHOOLY III,4 T. W. LYONS,5 L. P. TOMSHO,3 S. C. SCHUSTER,3,6 J. OVERMANN,7 D. A. BRYANT,3,6,8 A. PEARSON2 AND J. L. MACALADY1 1Department of Geosciences, Penn State Astrobiology Research Center (PSARC), The Pennsylvania State University, University Park, PA, USA 2Department of Earth and Planetary Sciences, Harvard University, Cambridge, MA, USA 3Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA 4Department of Earth Sciences, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA 5Department of Earth Sciences, University of California, Riverside, CA, USA 6Singapore Center for Environmental Life Sciences Engineering, Nanyang Technological University, Nanyang, Singapore 7Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany 8Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA ABSTRACT Mahoney Lake represents an extreme meromictic model system and is a valuable site for examining the organisms and processes that sustain photic zone euxinia (PZE). A single population of purple sulfur bacte- ria (PSB) living in a dense phototrophic plate in the chemocline is responsible for most of the primary pro- duction in Mahoney Lake. Here, we present metagenomic data from this phototrophic plate – including the genome of the major PSB, as obtained from both a highly enriched culture and from the metagenomic data – as well as evidence for multiple other taxa that contribute to the oxidative sulfur cycle and to sulfate reduction.
    [Show full text]
  • Supporting Information
    Supporting Information Musat et al. 10.1073/pnas.0809329105 SI Text bottles at in situ light and temperature conditions for 4, 8, and Method Development and Testing. Before application to environ- 12 h. In situ light and temperature conditions were created in the mental samples, the HISH-SIMS procedure was tested using a laboratory based on multiple measurements of light intensities mixture of 2 bacterial cultures, E. coli, a gammaproteobacterium and temperature performed annually and seasonally in the grown on 13C-labeled glucose as sole carbon source, and Azo- chemocline of Lake Cadagno between 1994–2007 [e.g., (5–7), arcus sp., a betaproteobacterium grown without labeled sub- this study]. In situ temperature was maintained between 5 to 8 °C strate. The cultures were fixed and then mixed, filtered on using a mix of water and ice under light intensities of 10–20 ␮mol Ϫ Ϫ gold-palladium coated filters, and hybridized with a horseradish m 2 s 1 as previously described (5, 7). Subsamples were taken peroxidase (HRP)-labeled oligonucleotide probe targeting the for bulk measurements of nitrogen and carbon from all three 23S rRNA gene of the Gammaproteobacteria. Following the incubation time points and only from a 12-h incubation exper- deposition of fluorine-containing tyramides within the hybrid- iment for nanoSIMS analysis of single cells. Water samples for ized cells, filters were analyzed using nanoSIMS. In this way, only bulk measurements were filtered on 0.7 ␮m pore-size type GF/F a single nanoSIMS scan was required to quantify the incorpo- filters (Millipore). After freeze-drying and decalcifying with ration of 13C-glucose by individual cells and to identify these cells 37% hydrochloric acid, the GF/F filters were kept at room based on a signal conferred by rRNA-targeted oligonucleotide temperature until further processing.
    [Show full text]
  • Potential Role of Microbiome in Oncogenesis, Outcome Prediction
    Oral Oncology 99 (2019) 104453 Contents lists available at ScienceDirect Oral Oncology journal homepage: www.elsevier.com/locate/oraloncology Review Potential role of microbiome in oncogenesis, outcome prediction and therapeutic targeting for head and neck cancer T ⁎ Ester Orlandia,b, , Nicola Alessandro Iacovellib, Vincenzo Tombolinic, Tiziana Rancatid, Antonella Polimenie, Loris De Ceccof, Riccardo Valdagnia,d,g, Francesca De Felicec a Department of Radiotherapy 1, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milan, Italy b Department of Radiotherapy 2, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milan, Italy c Department of Radiotherapy, Policlinico Umberto I, “Sapienza” University of Rome, Rome, Italy d Prostate Cancer Program, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milan, Italy e Department of Oral and Maxillo Facial Sciences, Policlinico Umberto I, “Sapienza” University of Rome, Italy f Integrated Biology Platform, Department of Applied Research and Technology Development, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milan, Italy g Department of Oncology and Hemato-Oncology, University of Milan, Milan, Italy ARTICLE INFO ABSTRACT Keywords: In the last decade, human microbiome research is rapidly growing involving several fields of clinical medicine Head and neck cancer and population health. Although the microbiome seems to be linked to all sorts of diseases, cancer has the Biomarkers biggest potential to be investigated. Microbiome Following the publication of the National Institute of Health - Human Microbiome Project (NIH-HMP), the Microbiota link between Head and Neck Cancer (HNC) and microbiome seems to be a fast-moving field in research area. Oncogenesis However, robust evidence-based literature is still quite scarce. Nevertheless the relationship between oral mi- Radiotherapy Immunotherapy crobiome and HNC could have important consequences for prevention and early detection of this type of tumors.
    [Show full text]
  • 3. Jedna Z Krvných Skupín Systému AB0; 4. Skr. Bukálny. B – Symbo
    B – 1. symbol pre bel; 2. chem. značka prvku →bór; 3. jedna z krvných skupín systému AB0; 4. skr. bukálny. B – symbol pre hustotu magnetického toku. B. – skr. pre →Bacillus. B-ALP – skr. angl. bone alcalic phosphatase kostná alkalická fosfatáza. B-bunky – 1. syn. -bunky Langerhansových ostrovčekov; →pankreas; 2. syn. B-lymfocyty; →lymfocyty. B-komplex – multivitamínový prípravok vitamínov B. B-lymfocyty – [B podľa Fabriciovej burzy, imunol. orgánu vtákov] B bunky, druh lymfocytov, kt. sa zúčastňuje na humorálnej imunite (tvorbe protilátok) a niekt. ďalších imunitných funkciách. Povrchové molekuly sú CD 19,20. Receptorom pre antigén je membránový imunoglobulín. B-reťazec – jeden z polypeptidových reťazcov →inzulínu. B-vírus – 1. vírus →hepatitídy B.; 2. vírus zo skupiny Cercopithecine herpes virus 1. B-vlákna →nerv. b – skr. 1. pre barn; 2. skr. angl. born narodený; 3. skr. báza (genet. označenie dĺţky sekvencie nukleotidov, napr. 50 b = sekvencia 50 nukleotidov). b-vlna – pozit. kmit s vyskou amplitúdou v →elektroretinograme nasledujúci po vlne a, prejav komplexnej aktivity vrstvy bipolárnych buniek. – - – predpona označujúca 1. staršie označenie druhého atómu uhlíka v reťazci, na kt. sa pripája hlavná funkčná skupina, napr. kys. -hydroxymaslová správ. kys. 3-hydroxymaslová; 2. špecifická rotácia opticky aktívnej látky, napr. -D-glukóza; 3. orientácia exocyklického atómu al. skupiny, napr. cholest-5-en-3--ol; 4. plazmatický proteín, kt. migruje v elektroforéze v pruhu - lipoproteín; 5. člen série príbuzných chem. látok, najmä série stereoizomérov, izomérov, polymérov al. alotroických foriem, napr. -karotén; 6. -lúč. B2 – staršie označenie pre CD21. B4 – staršie označenie pre CD 19. B19 virus – ľudský parvovírus z čeľade Parvoviridae, značne rozšírený, vyvolávajúci obvykle inaparentné infekcie.
    [Show full text]
  • 974-Form.Pdf
    California Association for Medical Laboratory Technology Distance Learning Program ANAEROBIC BACTERIOLOGY FOR THE CLINICAL LABORATORY by James I. Mangels, MA, CLS, MT(ASCP) Consultant Microbiology Consulting Services Santa Rosa, CA Course Number: DL-974 3.0 CE/Contact Hour Level of Difficulty: Intermediate © California Association for Medical Laboratory Technology. Permission to reprint any part of these materials, other than for credit from CAMLT, must be obtained in writing from the CAMLT Executive Office. CAMLT is approved by the California Department of Health Services as a CA CLS Accrediting Agency (#0021) and this course is is approved by ASCLS for the P.A.C.E. ® Program (#519) 1895 Mowry Ave, Suite 112 Fremont, CA 94538-1766 Phone: 510-792-4441 FAX: 510-792-3045 Notification of Distance Learning Deadline All continuing education units required to renew your license must be earned no later than the expiration date printed on your license. If some of your units are made up of Distance Learning courses, please allow yourself enough time to retake the test in the event you do not pass on the first attempt. CAMLT urges you to earn your CE units early!. CAMLT Distance Learning Course # DL-974 1 © California Association for Medical Laboratory Technology Outline A. Introduction B. What are anaerobic bacteria? Concepts of anaerobic bacteriology C. Why do we need to identify anaerobes? D. Normal indigenous anaerobic flora; the incidence of anaerobes at various body sites E. Anaerobic infections; most common anaerobic infections F. Specimen collection and transport; acceptance and rejection criteria G. Processing of clinical specimens 1. Microscopic examination 2.
    [Show full text]
  • This Article Was Published in an Elsevier Journal. the Attached Copy
    This article was published in an Elsevier journal. The attached copy is furnished to the author for non-commercial research and education use, including for instruction at the author’s institution, sharing with colleagues and providing to institution administration. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier’s archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright Author's personal copy Available online at www.sciencedirect.com Geochimica et Cosmochimica Acta 72 (2008) 1396–1414 www.elsevier.com/locate/gca Okenane, a biomarker for purple sulfur bacteria (Chromatiaceae), and other new carotenoid derivatives from the 1640 Ma Barney Creek Formation Jochen J. Brocks a,*, Philippe Schaeffer b a Research School of Earth Sciences and Centre for Macroevolution and Macroecology, The Australian National University, Canberra, ACT 0200, Australia b Laboratoire de Ge´ochimie Bio-organique, CNRS UMR 7177, Ecole Europe´enne de Chimie, Polyme`res et Mate´riaux, 25 rue Becquerel, 67200 Strasbourg, France Received 20 June 2007; accepted in revised form 12 December 2007; available online 23 December 2007 Abstract Carbonates of the 1640 million years (Ma) old Barney Creek Formation (BCF), McArthur Basin, Australia, contain more than 22 different C40 carotenoid derivatives including lycopane, c-carotane, b-carotane, chlorobactane, isorenieratane, b-iso- renieratane, renieratane, b-renierapurpurane, renierapurpurane and the monoaromatic carotenoid okenane.
    [Show full text]
  • Susceptibility and Resistance Data
    toku-e logo For a complete list of references, please visit antibiotics.toku-e.com Levofloxacin Microorganism Genus, Species, and Strain (if shown) Concentration Range (μg/ml)Susceptibility and Aeromonas spp. 0.0625 Minimum Inhibitory Alcaligenes faecalis 0.39 － 25 Bacillus circulans Concentration0.25 － 8 (MIC) Data Bacillus subtilis (ATCC 6051) 6.25 Issue date 01/06/2020 Bacteroides capillosus ≤0.06 － >8 Bacteroides distasonis 0.5 － 128 Bacteroides eggerthii 4 Bacteroides fragilis 0.5 － 128 Bacteroides merdae 0.25 － >32 Bacteroides ovatus 0.25 － 256 Bacteroides thetaiotaomicron 1 － 256 Bacteroides uniformis 4 － 128 Bacteroides ureolyticus ≤0.06 － >8 Bacteroides vulgatus 1 － 256 Bifidobacterium adolescentis 0.25 － >32 Bifidobacterium bifidum 8 Bifidobacterium breve 0.25 － 8 Bifidobacterium longum 0.25 － 8 Bifidobacterium pseudolongum 8 Bifidobacterium sp. 0.25 － >32 Bilophila wadsworthia 0.25 － 16 Brevibacterium spp. 0.12 － 8 Brucella melitensis 0.5 Burkholderia cepacia 0.25 － 512 Campylobacter coli 0.015 － 128 Campylobacter concisus ≤0.06 － >8 Campylobacter gracilis ≤0.06 － >8 Campylobacter jejuni 0.015 － 128 Campylobacter mucosalis ≤0.06 － >8 Campylobacter rectus ≤0.06 － >8 Campylobacter showae ≤0.06 － >8 Campylobacter spp. 0.25 Campylobacter sputorum ≤0.06 － >8 Capnocytophaga ochracea ≤0.06 － >8 Capnocytophaga spp. 0.006 － 2 Chlamydia pneumonia 0.125 － 1 Chlamydia psittaci 0.5 Chlamydia trachomatis 0.12 － 1 Chlamydophila pneumonia 0.5 Citrobacter diversus 0.015 － 0.125 Citrobacter freundii ≤0.00625 － >64 Citrobacter koseri 0.015 －
    [Show full text]
  • Dark Aerobic Sulfide Oxidation by Anoxygenic Phototrophs in the Anoxic Waters 2 of Lake Cadagno 3 4 Jasmine S
    bioRxiv preprint doi: https://doi.org/10.1101/487272; this version posted December 6, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Dark aerobic sulfide oxidation by anoxygenic phototrophs in the anoxic waters 2 of Lake Cadagno 3 4 Jasmine S. Berg1,2*, Petra Pjevac3, Tobias Sommer4, Caroline R.T. Buckner1, Miriam Philippi1, Philipp F. 5 Hach1, Manuel Liebeke5, Moritz Holtappels6, Francesco Danza7,8, Mauro Tonolla7,8, Anupam Sengupta9, , 6 Carsten J. Schubert4, Jana Milucka1, Marcel M.M. Kuypers1 7 8 1Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, 28359 Bremen, Germany 9 2Institut de Minéralogie, Physique des Matériaux et Cosmochimie, Université Pierre et Marie Curie, CNRS UMR 10 7590, 4 Place Jussieu, 75252 Paris Cedex 05, France 11 3Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, University of Vienna, 1090 12 Vienna, Austria 13 4Eawag, Swiss Federal Institute of Aquatic Science and Technology, Kastanienbaum, Switzerland 14 5Department of Symbiosis, Max Planck Institute for Marine Microbiology, 28359 Bremen, Germany 15 6Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung, Am Alten Hafen 26, 27568 16 Bremerhaven, Germany 17 7Laboratory of Applied Microbiology (LMA), Department for Environmental Constructions and Design (DACD), 18 University of Applied Sciences and Arts of Southern Switzerland (SUPSI), via Mirasole 22a, 6500 Bellinzona, 19 Switzerland 20 8Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland 21 9Institute for Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, ETH 22 Zurich, 8093 Zurich, Switzerland.
    [Show full text]
  • Spatio-Temporal Distribution of Phototrophic Sulfur Bacteria in the Chemocline of Meromictic Lake Cadagno (Switzerland)
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by RERO DOC Digital Library FEMS Microbiology Ecology 43 (2003) 89^98 www.fems-microbiology.org Spatio-temporal distribution of phototrophic sulfur bacteria in the chemocline of meromictic Lake Cadagno (Switzerland) Mauro Tonolla a, Sandro Peduzzi a;b, Dittmar Hahn b;Ã, Ra¡aele Peduzzi a a Cantonal Institute of Microbiology, Microbial Ecology (University of Geneva), Via Giuseppe Bu⁄ 6, CH-6904 Lugano, Switzerland b Department of Chemical Engineering, New Jersey Institute of Technology (NJIT), and Department of Biological Sciences, Rutgers University, 101 Warren Street, Smith Hall 135, Newark, NJ 07102-1811, USA Received 24 May 2002; received in revised form 26 July 2002; accepted 30 July 2002 First published online 4 September 2002 Abstract In situ hybridization was used to study the spatio-temporal distribution of phototrophic sulfur bacteria in the permanent chemocline of meromictic Lake Cadagno, Switzerland. At all four sampling times during the year the numerically most important phototrophic sulfur bacteria in the chemocline were small-celled purple sulfur bacteria of two yet uncultured populations designated Dand F. Other small- celled purple sulfur bacteria (Amoebobacter purpureus and Lamprocystis roseopersicina) were found in numbers about one order of magnitude lower. These numbers were similar to those of large-celled purple sulfur bacteria (Chromatium okenii) and green sulfur bacteria that almost entirely consisted of Chlorobium phaeobacteroides. In March and June when low light intensities reached the chemocline, cell densities of all populations, with the exception of L. roseopersicina, were about one order of magnitude lower than in August and October when light intensities were much higher.
    [Show full text]