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<I>Nephroma Helveticum ISSN (print) 0093-4666 © 2013. Mycotaxon, Ltd. ISSN (online) 2154-8889 MYCOTAXON http://dx.doi.org/10.5248/125.263 Volume 125, pp. 263–275 July–September 2013 Study on the phylogeny of Nephroma helveticum and allied species Hai-Ying Wang1*, Dai-Feng Jiang1, Yan-Hong Huang2, Pan-Meng Wang1 & Tao Li1 1Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, 250014, P. R. China 2 Food and Fermentation Engineering Key Lab of Shandong Province, Jinan 250013, P. R. China * Correspondence to: endolichen@gmail.com Abstract — Nephroma subhelveticum sp. nov. is described, supported by both morphological and molecular data as a species new to science. The nrDNA ITS sequence analysis indicates that N. subhelveticum and N. flavorhizinatum are allied species of N. helveticum, and that N. isidiosum derives from N. helveticum. Key words — Asia, China, evolution, lichen, taxonomy Introduction The species of Nephroma Ach. (Nephromataceae) have middle-sized foliose thalli, cyanobacteria (sometimes green algae) as their photobionts, apothecia produced on the lower surface, 8-spored asci, commonly 3-septate ascospores, and often abundant terpenoids (James & White 1987, Burgaz & Martínez 1999, Brodo et al. 2001, Wetmore & Nash 2002, Louwhoff 2009). This genus includes 37 species worldwide (Tian et al. 2011). Both Nephroma helveticum and N. isidiosum are cosmopolitan species with terpenoids, and only these two species in the genus have isidia (Brodo et al. 2001). Nephroma helveticum has lobules and flat isidia, while N. isidiosum has true cylindrical or branched isidia (Brodo et al. 2001). Nephroma flavorhizinatum also has lobules and terpenoids (Tian et al. 2011). The similar morphological and chemical characters indicate that these three species may be closely related. Recently some morphologically similar specimens that also have terpenoids, lobules, and very crisp thalli, were found from China. The nrDNA ITS sequences generated from these Nephroma representatives have been analysed so as to establish their phylogenetic relationships. 264 ... Wang & al. Table 1. GenBank sequences of Nephroma and Lobaria included in the molecular analyses. Species Origin GenBank no. N. helveticum China, Yunnan DQ001292 Macaronesia HQ455071 USA, southwest AY124119 China, Tibet AY124120 USA, northwest AY124124 N. subhelveticum South Korea DQ066705 (recorded as N. helveticum) South Korea DQ066706 China, central AY124118 China, central AY124122 China, northeast AY124127 Russia, northwest AY124128 N. parile Canada, east AY124148 N. resupinatum South Korea DQ066710 L. macaronesica Outgroup GU072745 L. pulmonaria GU072753 L. retigera EU626996 Materials & methods Morphology & chemistry The specimens studied are preserved in SDNU (Lichen Section of Botanical Herbarium, Shandong Normal University). The morphology and anatomy of the specimens were examined using an Olympus SZX16 stereomicroscope and Olympus BX61 compound microscope; morphological characters were photographed under Olympus with DP72. Lichen substances were identified using standardized thin layer chromatography techniques (TLC) with C system (Orange et al. 2010). Molecular analyses Taxon sampling — nrDNA ITS sequences were obtained from 41 Chinese Nephroma specimens; 13 additional Nephroma sequences and outgroup sequences from three Lobaria species were downloaded from GenBank (Table 1). PCR amplification and sequencing — Total DNA was extracted by the modified CTAB method (Rogers & Bendich 1988). E9 (ttgtacacaccgcccgt), NF1R (atccgaggtcaatcgtgt), NF2R (tgatccgaggtcaatcgt), and CL2R (tttcttttcctccgcttattga) were used for PCR amplification of the nrDNA ITS as primers. PCR reaction program: initial denaturation at 95°C for 3 min, followed by 35 cycles of 30 s denaturation at 94°C, 45 s annealing at 58°C, 1 min extension at 72°C, and completed with a final 8 min extension at 72°C. Products were purified with Gel Extraction Mini Kit (SABC). Shanghai Genecore Corp. carried out the sequencing reactions. Data analysis — The sequences were aligned using MAFFT version 7 (Katoh & Standley 2013). The aligned ITS matrix was edited manually and phylogenetic analyses were conducted using MEGA 5 (Tamura et al. 2011). The phylogenetic tree was inferred using the Minimum Evolution method (Rzhetsky & Nei 1992) and tested by 1000 bootstrap replications (Felsenstein 1985). The evolutionary distances were computed using the Maximum Composite Likelihood method (Tamura et al. 2004). All positions containing Nephroma subhelveticum sp. nov. (China) ... 265 alignment gaps and missing data were eliminated in pairwise sequence comparisons (Pairwise deletion option). A total of 606 positions comprised the final dataset. Morphology & chemistry Nephroma helveticum Ach., Lich. univ.: 523 (1810) Figs 1, 2 Thallus foliose, leathery. Lobes with stout lobules (0.5 × 1 mm) at margin. Upper surface brown; isidia and soredia absent; tomentum and pruina often present; lobules marginal and laminal, often branched. Upper cortex pale Figure 1. Nephroma helveticum (20080653). A: Thallus; B: Lobe with marginal lobules; C: Laminal lobules; D: Rough and tomentose dorsal surface of apothecium; E: Tomentum on lower surface; F: Apothecium immersed on lower surface. 266 ... Wang & al. brown, ca. 35 µm thick; photobiont Nostoc, ca. 65 µm thick; medulla white, with abundant crystals soluble in water, ca. 80 µm thick; lower cortex pale, ca. 30 µm thick. Lower surface brown, tomentose, paler and smooth at the margins; rhizines stubby, sparse. Apothecia common, immersed on lower surface at tips of lobes, kidney-shaped; disc dark brown, ≤7 mm in diameter; dorsal surface rough, with abundant clustered tomentum; margin usually crenulate. Epihymenium pale brown, without crystals, ca. 15 µm tall; hymenium hyaline, without crystals, ca. 40 µm tall; hypothecium pale brown, without crystals, ca. 35 µm tall. Paraphyses simple. Asci clavate, I+ blue, 8-spored; ascospores pale brown, 3-septate, 15–20 × 3–4 µm. Pycnidia not seen. Spot tests — Thallus upper surface: K–, C–, KC–, PD–; medulla: K–, C–, KC–, PD–. Secondary metabolites — N1, N2, ± other lichen substances (Fig. 3). Ecology & distribution in China— on bark or moss in Guizhou, Jilin, Shanxi, Shaanxi, Sichuan, Tibet, and Yunnan. Specimens examined — CHINA. Guizhou, Kaili, Mt. Leigong, alt. 1027 m, on bark, 2 Nov 2009, H.Y. Wang 20102726. Jilin, Wangqing, Mt. Tulaopoding, alt. 1100 m, on moss, 22 Jul 2012, H.Y. Wang 20129292. Shanxi, Ningwu, Mt. Luya, alt. 2500 m, on bark, 26 Aug 2011, H.Y. Wang 20121819A. Shaanxi, Meixian, Mt. Taibai, alt. 2100 m, on bark, 2 Aug 2005, W. Fu 20083001; 17 Jun 2011, Z.L. Huang 20114881(GenBank JX867681); alt. 2150 m, on bark, 17 Jun 2011, Z.L. Huang 20114339 (GenBank JX867682); alt. 2200 m, on bark, 2 Aug 2005, W. Fu 20080332, 20100571; alt. 2700 m, on bark, 17 Jun 2011, Z.L Huang 20114639K; alt. 2950 m, on bark, 17 Jun 2011, Z.L. Huang 20114717 (GenBank JX867703). Sichuan, Litang, alt. 4200 m, on bark, 5 Nov 2008, H.Y. Wang 20080388; Litang, Mt. Kazila, alt. 4700 m, on bark, 7 Nov 2008, H.Y. Wang 20080323, 20080354. Tibet, Nyingchi, Dongjiuxiang, alt. 2550 m, on bark, 17 Jul 2011, Y.L. Cheng 20114928, 20117991 (GenBank JX867707), 20117991A (GenBank JX867710), 20117991C (GenBank JX867713), 20118005, 20118005A, 20118005D, 20118005E, 20118005F (GenBank JX867712), 20118006B (GenBank JX867704), 20118006C (GenBank JX867683), 20118011, 20118044, 20118255A (GenBank JX867709), 20118300 (GenBank JX867705), 20118545; Mt Sejila, alt. 4100 m, on bark, 20 Oct 2007, G.Y. Han 20072907; alt. 4500 m, on bark, 20 Jul 2011, Y.L. Cheng 20118118A, 20118461, 20118729A (GenBank JX867706), 20118729E; Lulangzhen, observation station, alt. 2550 m, on bark, 14 Jul 2011, D.F. Jiang 20119099. Yunnan, Shangri-La, Tianshengqiao, alt. 3500 m, on bark, 3 Nov 2008, H.Y. Wang 20080653 (GenBank JX867708), 20081370, 20081391, 20081515, 20081715, 20081885, 20082058, 20082065, 20082181, 20082185, 20082213, 20082264, 20082270, 20082291, 20082355, 20102930, 20083063, 20083097, 20083312, 20083385, 20083861, 20086001, 20086011; Mt. Shika, alt. 4300 m, on bark, 2 Nov 2008, H.Y. Wang 20081991, 20082033, 20082218, 20083336, 20086017; Yulong, Mt. Laojun, alt. 4000 m, on bark, 6 Nov 2009, H.Y. Wang 20100564 (GenBank JX867711); 7 Nov 2009, H.Y. Wang 20090001, 20100562; Jianchuan, Mt. Shibao, alt. 2600 m, on bark, 31 Oct 2008, H.Y. Wang 20083409; Lijiang, Mt. Yulong, alt. 3200 m, on bark, 7 Nov 2009, M. Li 20102729A. Comments — Nephroma helveticum is considered to produce flat isidia (Brodo et al. 2001). However, we did not find true isidia on our specimens. The lobules of N. helveticum from China are varied (Fig. 2 I–N), sometimes very narrow and Nephroma subhelveticum sp. nov. (China) ... 267 Figure 2. Nephroma helveticum (A–H, 20080653; I, 20080323; J, 20118011; K, 20118005D; L, 20118729E; M, 20080388; N, 20100564). A: Section of thallus; B: Crystals in thallus; C: Section of apothecium; D: Crystals in apothecium; E: Ascus and ascospores; F: Iodine reaction of ascus and paraphyses; G: Paraphyses; H: Ascospores; I–N: Various laminal lobules. isidium-like but always dorsiventral. Although N. helveticum and N. isidiosum have similar secondary substances (TLC, Fig. 3), N. isidiosum can be clearly separated by its typical cylindrical to coralloid isidia and lack of apothecia. 268 ... Wang & al. Figure 3. TLC results: A: UV254 nm before acid; B: Sunlight after acid and heating; C: UV365 nm after acid and heating. Nephroma helveticum (1, 20100564; 2, 20114717; 3, 20118255A; 4, 20117991C; 5, 20118300;
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