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Ana Cristina Oltramari Toledo.Pdf UNIVERSIDADE ESTADUAL DE PONTA GROSSA SETOR DE CIÊNCIAS BIOLÓGICAS E DA SAÚDE PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS ANA CRISTINA OLTRAMARI TOLEDO DESENVOLVIMENTO, CARACTERIZAÇÃO E AVALIAÇÃO DAS ATIVIDADES BIOLÓGICAS DE NANOPARTÍCULAS DE PRATA E DE OURO, OBTIDAS POR SÍNTESE VERDE, A PARTIR DO EXTRATO AQUOSO DAS SEMENTES DE Pterodon emarginatus Vogel (Fabaceae) ASSOCIADAS À GENTAMICINA E AO ÁCIDO HIALURÔNICO PONTA GROSSA 2021 ANA CRISTINA OLTRAMARI TOLEDO DESENVOLVIMENTO, CARACTERIZAÇÃO E AVALIAÇÃO DAS ATIVIDADES BIOLÓGICAS DE NANOPARTÍCULAS DE PRATA E DE OURO, OBTIDAS POR SÍNTESE VERDE, A PARTIR DO EXTRATO AQUOSO DAS SEMENTES DE Pterodon emarginatus Vogel (Fabaceae) ASSOCIADAS À GENTAMICINA E AO ÁCIDO HIALURÔNICO Tese apresentada para a obtenção do título de doutora na Universidade Estadual de Ponta Grossa, Área de Fármacos, Medicamentos e Biociências Aplicadas à Farmácia. Orientadora: Profa. Dra. Josiane de Fátima Padilha de Paula PONTA GROSSA 2021 Toledo, Ana Cristina Oltramari T649 Desenvolvimento, caracterização e avaliação das atividades biológicas de nanopartículas de prata e de ouro, obtidas por síntese verde, a partir do extrato aquoso das sementes de Pterodon emarginatus Vogel (Fabaceae) associadas à gentamicina e ao ácido hialurônico / Ana Cristina Oltramari Toledo. Ponta Grossa, 2021. 169 f. Tese (Doutorado em Ciências Farmacêuticas - Área de Concentração: Fármacos, Medicamentos e Biociências Aplicadas à Farmácia), Universidade Estadual de Ponta Grossa. Orientadora: Profa. Dra. Josiane de Fátima Padilha de Paula. 1. Pterodon emarginatus Vogel. 2. Síntese verde. 3. Nanopartículas metálicas. 4. Atividades biológicas. I. Paula, Josiane de Fátima Padilha de. II. Universidade Estadual de Ponta Grossa. Fármacos, Medicamentos e Biociências Aplicadas à Farmácia. III.T. CDD: 615.321 Ficha catalográfica elaborada por Maria Luzia Fernandes Bertholino dos Santos- CRB9/986 Programa de Pós-Graduação em Ciências Farmacêuticas Programa de Pós-Graduação em PttOGft"".DliPÓ5-OAfoOU"ÇI.O (11. ClEotClI'S F","""CEUTlCAS Ciências Farmacêuticas Associação Ampla entre a Universidade Estadual do Centro-Oeste e a Universidade Estadual de Ponta Grossa Ponta Grossa, 04 de março de 2021. ATA DE EXAME DE DEFESA DE TESE DE DOUTORADO EM CIÊNCIAS FARMACÊUTICAS-ÁREA DE CONCENTRAÇÃO: FÁRMACOS, MEDICAMENTOS E BIOCIÊNCIAS APLICADAS À FARMÁCIA NÚMERO 03/2021 DA DOUTORANDA ANA CRISTINA OLTRAMARI TOLEDO REALIZADA NO DIA 04 DE MARÇO DE 2021, NA UNIVERSIDADE ESTADUAL DE PONTA GROSSA. Aos quatro dias de março de dois mil e vinte e um, às 14hOOmin, via videoconferência, na Universidade Estadual de Ponta Grossa (UEPG) em sessão pública sob a presidência da Professora Doutora Josiane de Fátima Padilha de Paula reuniu-se a Banca Examinadora de exame de defesa de tese de Doutorado em Ciências Farmacêuticas da doutoranda Ana Cristina Oltramari Toledo, na linha de pesquisa: Desenvolvimento e Controle de Fármacos, Medicamentos e Correlatos, constituída pela Professora Doutora Josiane de Fátima Padilha de Paula (UEPGIPR) e demais Doutores (membros titulares): Daniela Gaspardo Folquitto (CESCAGE/PR), Patricia Mathias Doll Boscardin (UEPGIPR), Patrícia Mazureki Campos (UEPG/PR) e Vanessa Cristina Godoy Jasinski (CESCAGE/PR). Iniciados os trabalhos, a presidência deu conhecimento aos membros da banca e ao candidato das normas que regem o exame de defesa de tese de Doutorado e definiu-se a ordem a ser seguida pelos examinadores para arguição. O título do trabalho foi: "DESENVOLVIMENTO, CARACTERIZAÇÃO E AVALIAÇÃO DAS ATIVIDADES BIOLÓGICAS DE NANOPARTÍCULAS DE PRATA E DE OURO, OBTIDAS POR SÍNTESE VERDE, A PARTIR DO EXTRATO AQUOSO DAS SEMENTES DE Pterodon emarginatus Vogel (FABACEAE) ASSOCIADAS À GENTAMICINA E AO ÁCIDO IDALURÔNICO". Encerrada a defesa, a banca considerou 4p R.O V H DA a tese, considerado como requisito parcial para a obtenção do título de Doutor em Ciências Farmacêuticas. O aluno deverá entregar, no prazo de até 30 dias, a versão definitiva da tese de Doutorado, com as modificações sugeridas pelos membros da banca examinadora. Nada mais havendo a ser tratado, lavrou-se a presente ata que vai assinada pelos membros da Banca Examinadora. Observações (se necessário): --------------------------------------------------- Pró - Reitoria de Pesquisa e Pós- Graduação Programa de Pós-Graduação em Ciências Farmacêuticas, Fármacos, medicamentos e biociências aplicadas à Farmácia - Fone (42) 3220-3337- [email protected] Av. Carlos Cavalcanti, 4748 - Ponta Grossa - Paraná - Brasil - CEP: 84030-900 ~cyoeste ~e<:' ~ Programa de iUIICENTRO Pós-Graduação em Ciências Farmacêuticas Programa de ~ pública e tratulta Pós-Graduação em '?o ••••• PPOGR"'''''' Dfi P06.yIU.()U;t,CAO l>.o 11MCIEIfCIA. FAlUilACf"UTlCAS ~SJê.\!U(\ Ciências Farmacêuticas Associação Ampla entre a Universidade Estadual do Centro-Oeste e a Universidade Estadual de Ponta Grossa Alteração de título: sim Dnão ~ Novo título: ---------------------------------------------------------------------- Josiane de Fátima Padilhí de Paula 1 - (DEF AR - UEPG) Presidente &.u&!~ --D-a-n-i-el-a-G-a-s-p-ar-d~OlqUi~I""••2.'O.""'~~(~C~E-S-C-A-G-E---P-R-)---T-i-tu-la-r--- ~ Patricia Mathias Doll Boscardin 3 - (DEF AR-UEPG) - Titular ( Vanessa Cristina Godoy Jasinski 5 - Pró - Reitoria de Pesquisa e Pós- Graduação Programa de Pós-Graduação em Ciências Farmacêuticas, Fármacos, medicamentos e biociências aplicadas à Farmácia - Fone (42) 3220-3337- [email protected] Av. Carlos Cavalcanti, 4748 - Ponta Grossa - Paraná - Brasil- CEP: 84030-900 Dedico à minha família. Meu esposo Alceu pelo suporte emocional e técnico, presença constante e compreensão. Ao filho Mateus que colaborou com seu conhecimento em física, matemática e química na solução de dúvidas surgidas durante a pesquisa, também pela compreensão pelas minhas ausências, meu amor incondicional. AGRADECIMENTOS A Deus (Pai, Filho e Espírito Santo) pelo dom da vida, pela presença contínua em todos os momentos dando forças e iluminando meu caminho. Às minhas mães Maria, a do Céu pela sua intercessão junto a Deus pelos meus pedidos, a da terra que na experiência singela de seus 90 anos me ensinou a nunca desistir de orar e confiar, todos os dias com seu terço nas mãos. Ao meu Anjo da Guarda, minha doce companhia que nunca me abandonou e, naqueles momentos em que tudo parecia dar errado, no silêncio de meu coração, ele indicava o caminho. Ao meu querido pai, Alonso (in memorian) que com sacrifícios sempre procurou dar a melhor educação tanto para a formação de minha personalidade quanto a intelectual, muitas saudades meu pai. À professora Dra. Josiane de Fátima Padilha de Paula por aceitar ser minha orientadora e sempre me conduzir com toda a paciência, experiência e amizade pelos difíceis caminhos da pesquisa. À professora Dra. Patrícia Mathias Döll Boscardin sempre disponível a auxiliar ensinando, disponibilizando materiais e corrigindo todas as etapas dos ensaios de viabilidade celular. À professora Dra. Carla Cristine Kanunfre, do Departamento de Biologia Geral da Universidade Estadual de Ponta Grossa, que disponibilizou o Laboratório de Biologia Celular a fim de me auxiliar no desenvolvimento deste trabalho. Ao professor Dr. Luis Antonio Esmerino, do Departamento de Análises Clínicas e Toxicológicas da Universidade Estadual de Ponta Grossa, que possibilitou as análises da atividade antibacteriana no laboratório de Microbiologia Clínica bem como forneceu as cepas e auxiliou nas análises. Ao professor Prof. Dr. André Mauricio Brinatti, do Departamento de Física da Universidade Estadual de Ponta Grossa por ter gentilmente cedido o laboratório de Física Aplicada a Solos e Ciências Ambienteais para uma etapa de minha pesquisa. Ao professor Dr. Paulo Vitor Farago pela disponibilidade de fornecer equipamentos e reagentes para o desenvolvimento de algumas análises, bem como seu otimismo. À professora Dra. Jessica Mendes Nadal pelo incentivo e colaboração no desenvolvimento e avaliação das análises térmicas. À Colega de pós-graduação e de trabalho Luciane Mendes Monteiro pelo auxílio com a triagem fitoquímica e atividade antioxidante. À colega de pós-graduação Luiza Stolz Cruz pela disponibilidade em auxiliar em todas as etapas dos testes de viabilidade celular, seu otimismo, paciência e dedicação em todos os momentos foram imprescindíveis para que tudo funcionasse corretamente. Às Colegas - amigas de pós-graduação Bárbara Justus, Daniele Priscila da Silva Fardin de Assunção, pela presença, suporte e amizade em todos os momentos. Às colegas de pós-graduação Mackelly Simionatto pelo auxílio com equipamentos e reagentes no teste da atividade hemolítica e Marilú Swiech pelas trocas de experiência na pesquisa. Aos colegas de pós-graduação Joel Toríbio Espinoza e Guilherme dos Anjos Camargo pelo auxílio na interpretação de algumas análises. À Bruna de Lima Diniz por estar sempre disposta a auxiliar com seu conhecimento na formatação da tese. À funcionária e amiga de laboratório Miriam Soares que nunca mediu esforços para me ajudar no desenvolvimento da pesquisa, até mesmo na árdua tarefa de abrir os frutos de P. emarginatus. Ao técnico Maurício de Carvalho por ter auxiliado na etapa de preparação para as análises microbiológicas. Aos técnicos do LABMU e LABMU-SEBISA, Ana, Raquel, Sílvio, Vanessa, Virgínia, Traudi e Janaína, pelo atendimento cordial e pelas análises realizadas. Ao Programa de Pós-graduação em Ciências Farmacêuticas UNICENTRO- UEPG, pela oportunidade de crescimento técnico-cientifíco. Ao DEFAR pela liberação parcial de minhas atividades para poder realizar
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