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Universidade De São Paulo Faculdade De Zootecnia E Engenharia De Alimentos UNIVERSIDADE DE SÃO PAULO FACULDADE DE ZOOTECNIA E ENGENHARIA DE ALIMENTOS LAÍS GRIGOLETTO Genomic studies in Montana Tropical Composite cattle Pirassununga 2020 LAIS GRIGOLETTO Genomic studies in Montana Tropical Composite cattle Versão Corrigida Thesis submitted to the College of Animal Science and Food Engineering, University of São Paulo in partial fulfillment of the requirements for the degree of Doctor in Science from the Animal Biosciences program. Concentration area: Genetics, Molecular and Cellular Biology Supervisor: Prof. Dr. José Bento Sterman Ferraz Co-supervisor: Prof. Dr. Fernando Baldi Pirassununga 2020 Ficha catalográfica elaborada pelo Serviço de Biblioteca e Informação, FZEA/USP, com os dados fornecidos pelo(a) autor(a) Grigoletto, Laís G857g Genomic studies in Montana Tropical Composite cattle / Laís Grigoletto ; orientador José Bento Sterman Ferraz ; coorientador Fernando Baldi. -- Pirassununga, 2020. 183 f. Tese (Doutorado - Programa de Pós-Graduação em Biociência Animal) -- Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo. 1. beef cattle. 2. composite. 3. genomics. 4. imputation. 5. genetic progress. I. Ferraz, José Bento Sterman, orient. II. Baldi, Fernando, coorient. III. Título. Permitida a cópia total ou parcial deste documento, desde que citada a fonte - o autor UNIVERSIDADE DE SÃO PAULO Faculdade de Zootecnia e Engenharia de Alimentos Comissão de Ética no Uso de Animais DISPENSA DE ANÁLISE ÉTICA Comunicamos que o projeto de pesquisa abaixo identificado está dispensado da análise ética por utilizar animais oriundos de coleções biológicas formadas anteriormente ao ano de 2008, ano da promulgação da Lei nº 11.794/2008 – lei que estabelece procedimentos para o uso científico de animais. Ressaltamos que atividades realizadas na vigência da referida lei, ou que resulte em incremento do acervo biológico, devem ser submetidas à análise desta CEUA conforme disposto pelo Conselho Nacional de Controle de Experimentação Animal (CONCEA). Projeto: Genomic Studies in Montana tropical composite cattle. Finalidade: Pesquisa Acadêmica – Tese de Doutorado Pesquisador: Laís Grigoletto Pesquisador responsável: Prof. Dr. José Bento Sterman Ferraz (Dep. ZMV) Instituição: Faculdade de Zootecnia e Engenharia de Alimentos Informações adicionais: - . Pirassununga, 18 de agosto de 2020. Profa. Dra. CRISTIANE GONÇALVES TITTO Profa. Dra. DANIELE DOS SANTOS MARTINS Presidente da Comissão de Ética no Uso de Vice-Presidente da Comissão de Ética no Uso de Animais Animais Av. Duque de Caxias Norte, 225 - 13635-900 – Pirassununga, SP Fone: (19) 3565-6759 -- E-mail: [email protected] Universidade de São Paulo ATA DE DEFESA Aluno: 74135 - 9206875 - 2 / Página 1 de 1 Ata de defesa de Tese do(a) Senhor(a) Laís Grigoletto no Programa: Biociência Animal, do(a) Faculdade de Zootecnia e Engenharia de Alimentos da Universidade de São Paulo. Aos 10 dias do mês de julho de 2020, no(a) Sala do docente (GMAB) realizou-se a Defesa da Tese do(a) Senhor(a) Laís Grigoletto, apresentada para a obtenção do título de Doutora intitulada: "Estudos genômicos em bovinos de corte composto Montana tropical" Após declarada aberta a sessão, o(a) Sr(a) Presidente passa a palavra ao candidato para exposição e a seguir aos examinadores para as devidas arguições que se desenvolvem nos termos regimentais. Em seguida, a Comissão Julgadora proclama o resultado: Nome dos Participantes da Banca Função Sigla da CPG Resultado José Bento Sterman Ferraz Presidente FZEA - USP ______________ Heidge Fukumasu Titular FZEA - USP ______________ Juliana Petrini Titular UNIFAL - Externo ______________ Ricardo Vieira Ventura Titular FMVZ - USP ______________ Luiz Fernando Brito Titular PU - Externo ______________ Resultado Final: ______________ Parecer da Comissão Julgadora * Eu, Erica Cristina Mello Ferraz ____________________________________ , lavrei a presente ata, que assino juntamente com os(as) Senhores(as). Pirassununga, aos 10 dias do mês de julho de 2020. Heidge Fukumasu Juliana Petrini Ricardo Vieira Ventura Luiz Fernando Brito José Bento Sterman Ferraz Presidente da Comissão Julgadora * Obs: Se o candidato for reprovado por algum dos membros, o preenchimento do parecer é obrigatório. A defesa foi homologada pela Comissão de Pós-Graduação em ______________ e, portanto, o(a) aluno(a) _______________ jus ao título de Doutora em Ciências obtido no Programa Biociência Animal. __________________________________ Presidente da Comissão de Pós-Graduação To my lovely and supportive family. In memory of my grandmother's Maria Luiza and Cacilda (“Cacá”). I dedicate. ACKNOWLEDGEMENTS I truly believe that every person, situation, and the lesson come in our life for a reason. I am very blessed to have and met amazing people that I have learned with and can call then family and friends. I am very glad to no longer be the same person as I was in the past year. I’ve learned with each one and situation in my life. Also, I feel so realized for each decision I made in life until now. I was very lucky during my Ph.D. I met (and re-met) very special people, which did not only teach me about genetics and genomics but also taught me a new perspective and how to be a better person and chase my dreams. They helped me to not give up, even when I was scared and did not believe in myself. I could have never done half of what I did in my Ph.D. if I have not met them at some point in my path. There is a theory called six degrees of separation originated from a scientific study d that describes, in the world, a maximum of six bonds of friendship is necessary for two people to be connected, so on in the past five years, I may have more connections built. Thus, I would like to show here my thanks to some of the people that were part of these five (2 of masters and 3 of Ph.D.) tremendous years of my life. First of all, I would like to thank my family. My parents Renato and Angela to give me the support, care, and they love to go through this intense cycle of my life. I do not have words to describe how I am thankful. To my brother, Dr. Renan, who shared the whole life with me, also be very protective for me, and of course, we divide the Ph.D. life together. Even more, I am so thankful to Renan and my sister in law Carolina, to my blessed and perfect nephew Bento. To all my uncles and aunts, and cousins. To my little cousin Gabi (“Pri”) to all the support! If I had the chance of choosing my family, I would choose all of you again! I would like to say a very big thank you to my advisor, Dr. José Bento Sterman Ferraz, for all support that he has given me since when I sent an email asking to be your Master student. That decision to give up my job and start this journey worth each second. Bento, you gave me a lot of opportunities that I could have never imagined. You were always there ready to help me with everything that I needed. You always helped me to achieve my dreams as if they were yours, and you believed in my potential even when I did not. You are the best advisor that I could have asked for, and I am very proud of being your student and called you as a friend (sometimes more like my second father). You have a special place in my life, and we need to have our barbecue as soon as possible. Thank you very much for everything that you have done for me. I admire you a lot for everything that you have been doing for the improvement of the beef cattle industry in Brazil. Dr. Luiz Fernando Brito, you have been the best person I have met in life. It was a pleasure to have the opportunity to stay close with you last year (2019-2020) doing part of my Ph.D. at Purdue University. I am very thankful for having trusted and believe in me. Thanks for being by my side helping, teaching, and cheering for me. You proved to me how far we can go if we work hard, and how life brings us good surprises when we do our best. But especially, our discussions/partnerships/projects/travels were fundamental in making me who I am today. I remember that the first thing you said to me was: “Lais, my job here, is to make you think”. And, Luiz every day that I need to do something, I always think why should I need to do this When? How? I am sure that we will continue to work together. Thanks for everything. Dra. Hinayah, you are an example of a professional and a person for me. Thanks for all your help, discussion, dinners, and friendship. I miss sharing my day and all my stories with you. I can have a piece of what it is to have a sister in my life. Elisangela, our kindness “Li”, you’ve been all my supporting and friend in this important cycle of my life. I could have never imagined that someone would be so nice and kind. You were the most sweetness surprise from life, and I have sure that you will always be present in my life. Thank you for all these years, coffee times, advisers, care, listening, and other more special moments. Dr. Fernando Baldi, you helped me a lot in my early beginning in the academic field. Thanks for all these years. I’ve learned a lot from you. Dr. Joanir Pereira Eler, thanks for sharing all the data and your knowledge with me. Dr. Miguel Henrique Almeida Santana, I would like to thank you for always have the right words and trust. And, of course, for your friendship. You are like a brother to me.
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