DOCSLIB.ORG

18 INTERNATIONAL CONFERENCE on the CELL and MOLECULAR BIOLOGY of CHLAMYDOMONAS Carnegie Institution for Science, Washington

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
18 INTERNATIONAL CONFERENCE on the CELL and MOLECULAR BIOLOGY of CHLAMYDOMONAS Carnegie Institution for Science, Washington 18th INTERNATIONAL CONFERENCE ON THE CELL AND MOLECULAR BIOLOGY OF CHLAMYDOMONAS Carnegie Institution for Science, Washington DC. June 17-21, 2018 Arthur Grossman – Chair Stephen King – Co-Chair 1 SUPPORTED BY 2 3 Program For "18th International Conference on the Cell and Molecular Biology of Chlamydomonas”, Washington DC, June 17-21, 2018 SUNDAY – June 17 Registration 4:00-7:30 PM Public talk - 7:30-8:30 PM Dr. Susan Dutcher - Washington University – Division of Biology and Biomedical Sciences From Algal Motility to Human Disease Question and Answer 8:30-9:00 PM Reception 9:00-10:00 PM MONDAY June 18 Light Breakfast 8:30 AM Session 1. Structure-Function of the Flagella Introduction (A. Grossman and S. King) 9:00-9:10 AM George Witman – Chair 9:10-9:20 AM Overview: Emerging Areas of Flagella Biology Gang Fu 9:20-9:50 AM Cryo-electron Tomography Provides New Insights to the Structure of Chlamydomonas Flagella Win Sale 9:50-10:10 AM Mechanisms of Assembly and Transport of the Flagellar Inner Dynein Arms Elizabeth Smith 10:10-10:30 AM A Role for PACRG in Ciliary Motility Coffee Break 10:30-11:00 AM Karl Lechtreck 11:00- 11:20 AM Basal Bodies as Organizing Centers for Intraflagellar Transport (IFT) Ahmet Yildiz 11:20-11:40 AM Dynamics of the IFT Machinery at the Tip of Chlamydomonas Flagella TALKS FROM ABSTRACTS Mareike A. Jordan 11:40-11:50 AM The Cryo-EM Structure of Intraflagellar Transport Trains Reveals How Its Motors Avoid Engaging in a Tug-of-War Tomohiro Kubo 11:50-12:00 PM A Microtubule-Dynein Tethering Complex Regulates the Axonemal Inner Dynein f (I1) Peeyush Ranjan 12:00-12:10 PM En route from the Plasma Membrane to the Ciliary Membrane during Signaling, a Membrane Signaling Protein is 4 Internalized, Aligns Along Cytoplasmic Microtubules, and Returns to the Peri-ciliary Plasma Membrane Discussion for Abstract Talks 12:10-12:20 PM Lunch 12:20-1:50 PM Session 2. Developing Technologies Ursula Goodenough Chair 1:40-1:50 PM Overview: New Resources to Probe Chlamydomonas Biology Peter Hegemann - Plenary Lecture 1:50-2:30 PM Advanced Biophysics and Reverse Genetics for Sensory Photoreceptor Characterization Ben Engel 2:30-2:50 PM Exploring the Molecular Landscape of Chlamydomonas with in situ Cryo-electron Tomography Attila Molnar 2:50-3:10 PM Efficient Targeted DNA Editing and Replacement Using Cpf1 Ribonucleoproteins and Single- Stranded DNA Martin Jonikas (Workshop) 3:10-3:50 PM Discussion on the Future of the Chlamydomonas Mutant Library Project Coffee Break 3:50-4:00 PM Posters Session 1 4:00-6:00 PM Free Time TUESDAY June 19 Light Breakfast 8:30 AM Session 3. Plastid Structure, Function and Biogenesis Francis-André Wollman - Chair 9:00-9:10 AM Overview Wojciech Nawrocki 9:10-9:30 AM The Mechanism of Cyclic Electron Flow around Photosystem I Setsuko Wakao 9:30-9:50 AM Acetate-Requiring Mutants Revisited: Sequencing and Comparative Transcriptomics Towards Discovering Photosynthesis Genes Guillaume Allorent 9:50-10:10 AM Regulation of Light Acclimation by UV-B Photoperception in Chlamydomonas reinhardtii Alexandra Viola-Bohne - 10:10-10:30 AM Metabolic Control of Chloroplast Gene Expression Coffee Break 10:30-10:50 AM Shai Saroussi 10:50-11:10 AM Dynamics of Photosynthetic Alternative Electron Transport 5 Felix Willmund 11:10-11:30 AM Quantitative Analysis of Chlamydomonas Chloroplast Translation by Ribosome- Profiling Talks from Abstracts Michael Hippler 11:30-11:40 AM Structure and Function of Photosystem I Complexes and Potential Implications on Photosynthetic Electron Transport Regulation in Microalgae Xiaobo Li 11:40-11:50 PM A Systems Biology Study Reveals a Global View of Genes Required for Eukaryotic Photosynthesis Marcello De Mia 11:50-12:00 PM Nitric Oxide Plays a Role in the Remodeling of the Photosynthetic Apparatus under Sulfur Starvation in Chlamydomonas reinhardtii Discussion for abstract talks 12:00-12:10 PM Lunch/free time 12:10-4:00 PM Posters Session 2 4:00-6:00 PM WEDNESDAY June 19 Light Breakfast 8:30 AM Session 4. Metabolic Processes Pierre Cardol - Chair 9:00-9:10 AM Overview Stephane Lemaire 9:10-9:40 AM Redox Regulation of Carbon Fixation and Stress Responses in Chlamydomonas Dimitris Petroutsos 9:40-10:00 Regulation of Photosynthesis by Carbon Metabolism in C. reinhardtii - Organization of the Chlamydomonas Pyrenoid Xenie Johnson 10:00-10:20 AM Interactions between Carbon Metabolism and the Alternative Electron Transport Pathways of Photosynthesis Coffee Break 10:20-10:50 AM Anja Hemschemeier 10:50- 11:10 AM (Low-oxygen) Stress in Chlamydomonas – The Role(s) of the Soluble Guanylate Cyclase CYG12 Talks from Abstracts Elisabeth Hommel 11:10-11:20 AM A Giant Type I Polyketide Synthase Participates in Zygospore Maturation in 6 Chlamydomonas reinhardtii Kristen Holbrook 11:20-11:30 PM Visualization of Metal Sequestration and Homeostasis Yonghua Li-Beisson 11:30-11:40 PM Reverse Coupling from Peroxisome to Chloroplast Impacts Lipid Homeostasis and Chloroplast Metabolism Discussion for Abstract Talks 11:40-11:50 PM Devaki Bhaya 11:50-12:10 PM Synthetic Biology at NSF Lunch 12:10-1:50 PM Session 5. Cell Cycle, Photoperception, Heat and Circadian Control" Maria Mittag - Chair 1:50-2:00 PM Overview Fred Cross Plenary Lecture 2:00-2:40 PM Chlamydomonas Cell Cycle Genetics Masayuki Onishi 2:40-3:00 PM - Cleavage-Furrow Formation without F-Actin or Myosin – Rethinking Cytokinesis through Chlamydomonas Cell Division Ru Zhang 3:00-3:20 PM Identifying Genome-Wide Regulators of Heat Responses in Chlamydomonas by Using Quantitative Pooled Screens Daniela Strenkert 3:20-3:40 PM A Day in the Life of Chlamydomonas: A Systems Biology Approach of the Cell Cycle Carol Dieckmann 3:40-4:00 PM Choosing the Right Path to the Eyespot Posters Session 3 4:00-6:00 PM Free Time THURSDAY June 21 Light Breakfast 8:30 AM Session 6. Novel Studies and Developing Systems Olivier Vallon - Chair 9:00-9:10 AM Overview Pierre Crozet 9:10-9:30 AM Birth of a Photosynthetic Chassis: a MoClo Toolbox for Synthetic Biology in Chlamydomonas reinhardtii Lital Davidi 9:30-9:50 AM Orchestrated Response of Dunaliella for Coping with Iron Limitation Severin Sasso 9:50-10:10 AM Beware of Evil Soil Bacteria! Raj Luxmi 10:10-10:30 AM A Peptide Amidating Monooxygenase and Its Amidated Products Appear in Ciliary Ectosomes 7 Coffee Break 10:30- 11:00 AM Inmaculada Couso 11:00-11:20 AM TORC1 Mediates Phosphate Limitation in Chlamydomonas reinhardtii Melissa Roth 11:20-11:40 AM A Molecular Switch for Oxygenic Photosynthesis and Metabolism in a Unicellular Green Alga Awards 11:40-11:50 AM Postdoc/Graduate Student Talks Olivier Vallon 11:50-12:10 Next Chlamydomonas Conference ABSTRACTS Regulation of Light Acclimation by UV-B Photoperception in C. reinhardtii (Talk) Allorent G.1; 2, Lefebvre-Legendre L.2, Chappuis R.2, Kuntz M.1, Truong TB.3, Niyogi KK.3, Petroutsos D.1, Finazzi G.1, Ulm R.2, Goldschmidt-Clermont M.2 1Cell and Plant Physiology laboratory, CEA Grenoble, Grenoble, France; 2 Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland and 3 Howard Hughes Medical Institute, Department of Plant and Microbial Biology, University of California, Berkeley, CA; Contact: Guillaume Allorent, [email protected]. Sunlight is essential for phototrophs, being a substrate for photosynthesis and a developmental signal. When photosynthetic organisms are exposed to high light, a wide range of mechanisms are activated to dissipate excess absorbed energy and protect the photosynthetic machinery from photodamage. These processes, mainly associated with photosystem II (PSII), are referred to as Non-Photochemical Quenching mechanisms (or NPQ). The most prominent NPQ component in plants and green algae is qE, the energy-dependent component of NPQ, which corresponds to a thermal dissipation of excess energy. UV is an intrinsic component of the sunlight spectrum, which severely impacts photosynthetic organisms mainly via its UV-B component. UV-B is sensed by the UV-B-specific photoreceptor UVR8 and regulates several biological functions. The impact of UV-B radiation on photosynthesis is becoming a major challenge in plant biology since it affects crop productivity but also aquatic ecosystems. Here, we report in the green alga Chlamydomonas reinhardtii that UVR8 induces accumulation of specific members of the light-harvesting complex (LHC) superfamily that contribute to qE, in particular LHC Stress-Related 1 (LHCSR1) and Photosystem II Subunit S (PSBS). The capacity for qE is strongly induced by UV-B, although the patterns of qE-related proteins accumulating in response to UV-B or to high light are clearly different. The competence for qE induced by acclimation to UV-B markedly contributes to photoprotection upon subsequent exposure to high light. Our study reveals an anterograde link between photoreceptor-mediated signaling in the nucleocytosolic compartment and the photoprotective regulation of photosynthetic activity in the chloroplast. A Chloroplast DnaJ-like Chaperone in Green Alga Chlamydomonas reinhardtii (Poster P1) Richa Amiya, Michal Shapira Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel. ([email protected]) 8 Assembly of most photosynthetic complexes is sensitive to the redox state within the chloroplast and involves a variety of chaperones, some showing target specificities by means not yet resolved. Our lab investigates the role of redox regulation on chaperone activities in the chloroplast, with special emphasis on a family of DnaJ-like chaperones contain four CXXCXGXG motifs, a known Zn-binding domain in DnaJ proteins. Members of this chaperone family bear resemblance to the Rubisco-specific bundle-sheath defective-2 (BSD 2) chaperone, all lacking the other typical Dna- J like domains (Brutnell, Sawers et al.
Load more

Recommended publications
  • Lateral Gene Transfer of Anion-Conducting Channelrhodopsins Between Green Algae and Giant Viruses
    bioRxiv preprint doi: https://doi.org/10.1101/2020.04.15.042127; this version posted April 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 5 Lateral gene transfer of anion-conducting channelrhodopsins between green algae and giant viruses Andrey Rozenberg 1,5, Johannes Oppermann 2,5, Jonas Wietek 2,3, Rodrigo Gaston Fernandez Lahore 2, Ruth-Anne Sandaa 4, Gunnar Bratbak 4, Peter Hegemann 2,6, and Oded 10 Béjà 1,6 1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel. 2Institute for Biology, Experimental Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, Berlin 10115, Germany. 3Present address: Department of Neurobiology, Weizmann 15 Institute of Science, Rehovot 7610001, Israel. 4Department of Biological Sciences, University of Bergen, N-5020 Bergen, Norway. 5These authors contributed equally: Andrey Rozenberg, Johannes Oppermann. 6These authors jointly supervised this work: Peter Hegemann, Oded Béjà. e-mail: [email protected] ; [email protected] 20 ABSTRACT Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity 1,2. Four ChR families are currently known. Green algal 3–5 and cryptophyte 6 cation-conducting ChRs (CCRs), cryptophyte anion-conducting ChRs (ACRs) 7, and the MerMAID ChRs 8. Here we 25 report the discovery of a new family of phylogenetically distinct ChRs encoded by marine giant viruses and acquired from their unicellular green algal prasinophyte hosts.
    [Show full text]
  • Understanding Physiological Adaptations, Metabolic Potential And
    UNDERSTANDING PHYSIOLOGICAL ADAPTATIONS, METABOLIC POTENTIAL AND ECOLOGY IN A NOVEL PHOTOAUTOTROPHIC ALGA FOR BIOFUEL PRODUCTION by Luisa Fernanda Corredor Arias A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Microbiology MONTANA STATE UNIVERSITY Bozeman, Montana November 2019 ©COPYRIGHT by Luisa Fernanda Corredor Arias 2019 All Rights Reserved ii DEDICATION To Ben, the love of my life, partner in crime and my happy place. Your love, support, devotion and kindness are beyond measure. To our little sunshine Lily. You turned my world into a wonderful rollercoaster of love and filled my life with joy and purpose. Para mi mamá Magda, por el inmenso amor, la dedicación y la ternura que siempre me brinda. Para mi papá Germán, quién despertó en mi la pasión por la ciencia y el conocimiento. iii ACKNOWLEDGEMENTS I would like to thank the Fulbright Scholarship Program for opening my mind to the world and its endless possibilities. It truly changed my life in the most positive ways. I would like to thank my advisor, Dr. Matthew Fields, for giving me the chance to be part of his lab, the opportunities he provided for my professional development and for his support and advice for the last seven years of my life. I am very grateful to my committee members, Drs. Robin Gerlach, Mensur Dlakic and Abigail Richards for their support and always taking the time to help me, teach me and guide me through grad school and science. A special thank you to former and current members of the Fields lab for their support and friendship, coming to work was always great fun.
    [Show full text]
  • 18S Ribosomal RNA Gene Phylogeny of a Colonial Volvocalean Lineage (Tetrabaenaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and Its Close Relatives
    J. Jpn. Bot. 91 Suppl.: 345–354 (2016) 18S Ribosomal RNA Gene Phylogeny of a Colonial Volvocalean Lineage (Tetrabaenaceae-Goniaceae-Volvocaceae, Volvocales, Chlorophyceae) and Its Close Relatives a,b, a,b a,b Takashi NAKADA *, Takuro ITO and Masaru TOMITA aInstitute for Advanced Biosciences, Keio University, Kakuganji, Tsuruoka, Yamagata, 997-0052 JAPAN; bSystems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa, Kanagawa, 252-0882 JAPAN; *Corresponding author: [email protected] (Accepted on January 19, 2016) The lineage of colonial green algae consisting of Tetrabaenaceae, Goniaceae, and Volvocaceae (TGV-clade) belongs to the clade Reinhardtinia within Volvocales (Chlorophyceae). Reinhardtinia is closely related to some species in the unicellular genera Chlamydomonas and Vitreochlamys. Although 18S rRNA gene sequences are preferred phylogenetic markers for many volvocalean species, phylogenetic relationships among the TGV-clade and its relatives have been examined mainly based on chloroplast genes and ITS2 sequences. To determine the candidate unicellular sister, 18S rRNA gene sequences of 41 species of the TGV-clade and its relatives were newly determined, and single and 6-gene phylogenetic analyses performed. No unicellular sister was determined by 18S rRNA gene analyses, but 6 unicellular clades and 11 ribospecies were recognized as candidates. Five of the candidate lineages and 27 taxa of the TGV-clade were examined by 6-gene phylogeny, revealing one clade including Chlamydomonas reinhardtii, Chlamydomonas debaryana, and Vitreochlamys ordinata to be more closely related than that containing Vitreochlamys aulata and Vitreochlamys pinguis. Key words: 18S rRNA, colonial, green algae, molecular phylogeny, unicellular, Volvocales. Tetrabaenaceae, Goniaceae, and cells) 8- to 50,000-celled genera (Pandorina, Volvocaceae constitute a colonial green Volvulina, Platydorina, Colemanosphaera, algal clade (TGV-clade) within Volvocales Yamagishiella, Eudorina, Pleodorina, and (Chlorophyceae), and include simple to Volvox).
    [Show full text]
  • 1 FATTY ACIDS in PHOTOTROPHIC and MIXOTROPHIC GYRODINIUM GALATHE- ANUM (DINOPHYCEAE) Adolf, J. E.1, Place, A. R.2, Lund, E.2, St
    PSA ABSTRACTS 1 1 Texas south jetty was completed between April 1999 FATTY ACIDS IN PHOTOTROPHIC AND and February, 2000. Species composition, seasonal pe- MIXOTROPHIC GYRODINIUM GALATHE- riodicity, and fluctuations in temperature and salinity ANUM (DINOPHYCEAE) were determined. This is the first comprehensive 1 2 2 study of benthic macroalgae conducted in Corpus Adolf, J. E. , Place, A. R. , Lund, E. , Stoecker, Christi Bay, which is shallow, turbid, and lacks natural 1 1,3 D. K. , & Harding, L. W., Jr hard substrate. Man-made jetties are necessary for 1Horn Point Lab, University of Maryland Center for suitable floral attachment. Macroalgae are affected by Environmental Science, Cambridge, MD 21613 USA; changes in salinity as freshwater inflows are followed 2Center of Marine Biotechnology, Baltimore, MD 21202 by periods of drought, which increase salinity. These USA; 3Maryland Sea Grant, University of Maryland, effects are most notable where freshwater enters at College Park, MD 20742 USA the south end near Oso Bay and at the north end at Nueces Bay. Previous Texas algal collections de- Fatty acids were measured in G. galatheanum grown scribed species of Enteromorpha, Ulva, Gelidium, and either phototrophically, or mixotrophically with Gracilaria as the most dominant plants of the area. Storeatula major (Cryptophyceae) as prey. G. galatheanum, This supports the current study with the additions of like many photosynthetic dinoflagellates, contains Hypnea musciformis and Centroceras clavulatum. Domi- high amounts of n-3 long-chain-polyunsaturated fatty nant plants at the Port Aransas jetty include Ulva fasci- acids (LC-PUFA) such as docosahexaenoic acid ata, Padina gymnospora, and Hypnea musciformis. The (DHA, 22:6n-3) and the hemolytic toxic fatty acid Rhodophyta including Gracilaria, Gelidium, and Centro- 18:5n-3.
    [Show full text]
  • Tracing the Evolution of the Arbuscular Mycorrhizal Symbiosis in the Plant Lineage
    Tracing the evolution of the arbuscular mycorrhizal symbiosis in the plant lineage Guru Vighnesh Radhakrishnan Thesis submitted for the degree of Doctor of Philosophy to the University of East Anglia Research conducted at the John Innes Centre September 2017 © This copy of thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with the author and that use of any information derived there from must be in accordance with current UK Copyright Law. In addition, any quotation or extract must include full attribution. Abstract The Arbuscular Mycorrhizal (AM) symbiosis is formed by ~80% of land plants with a specific group of soil fungi, the AM fungi. Through this symbiosis, plants obtain nutrients that they otherwise would not be able to access. Based on data from fossils and extant plants, it has been predicted that the AM symbiosis evolved in early land plants. Research in the past two decades utilising angiosperm model plant species has identified several plant genes that regulate the AM symbiosis. These studies have also revealed that these symbiosis genes are highly conserved in the angiosperms but whether this conservation extends to the non-flowering plants has not been explored. In the present study, a comprehensive phylogenetic analysis of the symbiosis genes was conducted, using genomic and transcriptomic data from the non-flowering plant lineages, to gain insights into the evolution of these genes in plants. The results of this analysis indicate that these genes evolved in a stepwise fashion. While some genes appeared in the algal ancestors of the charophytes, others appeared in the early land plant ancestors of liverworts.
    [Show full text]
  • PHYLUM Chlorophyta Phylum Chlorophyta to Order Level
    PHYLUM Chlorophyta Phylum Chlorophyta to Order Level P Chlorophyta C Bryopsidophyceae Chlorophyceae Nephroselmidophyceae Pedinophyceae Pleurastrophyceae Prasinophyceae Trebouxiophyceae Ulvophyceae O Bryopsidales Chlorocystidales Nephroselmidales Pedinomonadales Pleurastrales Pyramimonadales Chlorellales Cladophorales Volvocales Scourfieldiales Mamiellales Oocystales Codiolales Chaetopeltidales Chlorodendrales Prasiolales Trentepohliales Tetrasporales Prasinococcales Trebouxiales Ulotrichales Chlorococcales Pseudo- Ulvales Sphaeropleales scourfieldiales Siphonocladales Microsporales Dasycladales Oedogoniales Chaetophorales P Chlorophyta C Nephroselmidophyceae Pedinophyceae Pleurastrophyceae O Nephroselmidales Pedinomonadales Scourfieldiales Pleurastrales F Nephroselmidaceae Pedinomonadaceae Scourfieldiaceae Pleurastraceae G Anticomonas Anisomonas Scourfieldia Microthamnion Argillamonas Dioriticamonas Pleurastrosarcina Bipedinomonas Marsupiomonas Pleurastrum Fluitomonas Pedinomonas Hiemalomonas Resultor Myochloris Nephroselmis Pseudopedinomonas Sinamonas P Chlorophyta Prasinophyceae C O Pyramimonadales Mamiellales Chlorodendrales Prasinococcales Pseudoscourfieldiales F Polyblepharidaceae Mamiellaceae Chlorodendraceae Prasinococcaceae Pycnococcaceae Halosphaeraceae Monomastigaceae Mesostigmataceae G Polyblepharides Bathycoccus Prasinocladus Prasinococcus Pycnococcus Selenochloris Crustomastix Scherffelia Prasinoderma Pseudoscourfieldia Stepanoptera Dolichomastix Tetraselmis Sycamina Mamiella Mantoniella Prasinochloris Micromonas Protoaceromonas
    [Show full text]
  • The Probiotics of Biofuel: a Metagenomic Study of Microalgae Grown for Fuel Production
    ABSTRACT Title of Thesis: THE PROBIOTICS OF BIOFUEL: A METAGENOMIC STUDY OF MICROALGAE GROWN FOR FUEL PRODUCTION Samuel Russell Major, Master of Science, 2018 Thesis Directed By: Dr. Russell T. Hill, Professor, Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science Ponds in Frederick, MD were fertilized with chicken manure to increase the nutrient load in the water and stimulate microalgal growth. Nutrient analyses indicate that fertilization results in significant increases in the DOC, TDN, and TDP. The bacterial and eukaryotic microalgal communities were analyzed using 16S and 18S rRNA gene sequencing, respectively. Communities were analyzed pre-fertilization and for 15 days following fertilization. Molecular data reveals a decrease in diversity as microalgal blooms form. The microalgal density increased following fertilization, with enrichment for the Chlamydomonadales order. Prior to fertilization the bacterial communities were dominated by five phyla: Actinobacteria, Bacteroidetes, Cyanobacteria, Proteobacteria, and Verrucomicrobia. Dominant bacterial genera post-fertilization included Flavobacterium, Limnohabitans, and Polynucleobacter. Bacteria isolated from the ponds were screened for effects on Scenedesmus sp. HTB1 to identify bacteria that either enhance or inhibit microalgal growth. The growth- promoting bacteria were closely related to bacteria found to be enriched during microalgal bloom formation. THE PROBIOTICS OF BIOFUEL: A METAGENOMIC STUDY OF MICROALGAE GROWN FOR FUEL PRODUCTION by Samuel Russell Major Thesis submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Master of Science 2018 Advisory Committee: Professor Russell T. Hill, Chair Professor Feng Chen Associate Professor Yantao Li © Copyright by Samuel Russell Major 2018 Dedication I would like to dedicate this work to my mother and father for their unwavering support for all my endeavors.
    [Show full text]
  • Fay<Wei!Li! Department!Of!Biology! Duke!Unive
    ! ! Seeing!the!Light:!the!Origin!and!Evolution!of!Plant!Photoreceptors! by! Fay<Wei!Li! Department!of!Biology! Duke!University! ! Date:_______________________! Approved:! ! ___________________________! Kathleen!M.!Pryer,!Supervisor! ! ___________________________! Meng!Chen! ! ___________________________! Sönke!Johnsen! ! ___________________________! Corbin!Jones! ! ___________________________! Sarah!Mathews! ! Dissertation!submitted!in!partial!fulfillment!of! the!requirements!for!the!degree!of!Doctor!of!Philosophy!in!the!Department!of! Biology!in!the!Graduate!School! of!Duke!University! ! 2015! ! ! i v! ! ! ABSTRACT! Seeing!the!Light:!the!Origin!and!Evolution!of!Plant!Photoreceptors! by! Fay<Wei!Li! Department!of!Biology! Duke!University! ! Date:_______________________! Approved:! ! ___________________________! Kathleen!M.!Pryer,!Supervisor! ! ___________________________! Meng!Chen! ! ___________________________! Sönke!Johnsen! ! ___________________________! Corbin!Jones! ! ___________________________! Sarah!Mathews! ! An!abstract!of!a!dissertation!submitted!in!partial!fulfillment!of! the!requirements!for!the!degree!of!Doctor!of!Philosophy!in!the!Department!of! Biology!in!the!Graduate!School! of!Duke!University! ! 2015! i v! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! Copyright!by! Fay<Wei!Li! 2015! ! ! ! Abstract Plants!use!an!array!of!photoreceptors!to!measure!the!quality,!quantity,!and!direction!of! light!in!order!to!respond!to!ever<changing!light!environments.!Photoreceptors!not!only!determine! how!and!when!individual!plants!complete!their!life!cycles,!but!they!also!have!a!profound!and!
    [Show full text]
  • Ui Annotated Key to the Identification of Commonly Occurring and Dominant Genera of Algae Observed in the Phytoplankton of the United States
    ui Annotated Key to the Identification of Commonly Occurring and Dominant Genera of Algae Observed in the Phytoplankton of the United States GEOLOGICAL SURVEY WATER-SUPPLY PAPER 2079 An Annotated Key to the Identification of Commonly Occurring and Dominant Genera of Algae Observed in the Phytoplankton of the United States By PHILLIP E. GREESON GEOLOGICAL SURVEY WATER-SUPPLY PAPER 2079 UNITED STATES GOVERNMENT PRINTING OFFICE, WASHINGTON: 1982 UNITED STATES DEPARTMENT OF THE INTERIOR JAMES G. WATT, Secretary GEOLOGICAL SURVEY Dallas L. Peck, Director Library of Congress catalog-card No. 81-600168 For sale by the Superintendent of Documents, U.S. Government Printing Office Washington, D.C. 20402 CONTENTS Page Abstract_______________________________________ 1 Introduction _________________________________________ 1 Acknowledgment _ ___________________________________ 3 Taxonomic key to the identification of commonly occurring and dominant genera of algae observed in the phytoplankton of the United States ___________ 9 Descriptions of the genera _______________________________ 14 Chlorophyta ______________________________________ 14 Actinastrum __________________________________________________ 14 Ankistrodesmus _______________________________________________ 16 Chlamydomonas _______________________________________________ 18 Chodatella ____________________________________________________ 20 Coelastrum ___________________________________________________ 22 Cosmarium ___________________________________________________ 24 Crucigenia ___________________________________________________
    [Show full text]
  • Assessing the Persistence of Environmental DNA and Environmental RNA for Zooplankton Biodiversity Monitoring by Metabarcoding
    Assessing the persistence of environmental DNA and environmental RNA for zooplankton biodiversity monitoring by metabarcoding Michaela Harris Department of Biology McGill University, Montreal A thesis submitted to McGill University in partial fulfilment of the requirement of the degree of Master of Science Biology April 2019 © Michaela Harris, 2019 1 Table of Contents Abstract ................................................................................................................................. 5 Résumé .................................................................................................................................. 6 Index of Tables ..................................................................................................................... 8 Index of Figures .................................................................................................................... 9 Acknowledgements ............................................................................................................. 10 Contribution of Authors .................................................................................................... 11 General Introduction ......................................................................................................... 12 Literature Review .......................................................................................................... 13 Molecular Biomonitoring ............................................................................................. 13
    [Show full text]
  • One Thousand Plant Transcriptomes and the Phylogenomics of Green Plants
    Article https://doi.org/10.1038/s41586-019-1693-2 Supplementary information One thousand plant transcriptomes and the phylogenomics of green plants In the format provided by the One Thousand Plant Transcriptomes Initiative authors and unedited Nature | www.nature.com/nature Supplemental Methods Transcriptome data generation Sample acquisition: The 1000 plants initiative (1KP or onekp) analyzed 1342 RNA seq libraries from 1147 species including 1112 green plant species representing all of the major taxa within the Viridiplantae, including angiosperms, conifers, ferns, mosses, streptophytic algae and chlorophyte green algae (Supplementary Table 1). Because of the diversity of species, no one source could be used. Samples were provided by a global network of more than a hundred tissue and RNA providers (see author contributions) who obtained materials from a variety of sources, including field collection of wild plants, botanical gardens, greenhouses, laboratory specimens, and culture collections. Typically, samples were fast growing tissues such as young leaves or shoots, because these are expected to be largely comprised of live cells and therefore rich in RNA. However, some RNAs were derived from a mix of vegetative and reproductive tissues and we did not attempt to define specific standards on growth conditions, time of collection, or age of tissue. Vouchering system: The majority of the samples have corresponding vouchers deposited at various institutions, as convenient for the sample providers (http://www.onekp.com/samples/list.php). For the algal species, material from culture collections was sourced and the relevant collection identifiers are reported. Species names were validated with the Taxonomic Name Resolution Service1(http://tnrs.iplantcollaborative.org) or checked against other plant name databases such as Tropicos (http://www.tropicos.org).
    [Show full text]
  • On the Rediscovery of Volvox Perglobator (Volvocales, Chlorophyceae) and the Evolution of Outcrossing from Self-Fertilization
    Evolutionary Ecology Research — Volume 19 (2019) APPENDIX On the rediscovery of Volvox perglobator (Volvocales, Chlorophyceae) and the evolution of outcrossing from self-fertilization Erik R. Hanschen1,2,*, Dinah R. Davison2, Patrick J. Ferris2, Richard E. Michod2 1 Division of Bioscience, Los Alamos National Laboratory, Los Alamos, New Mexico 87544 2Department of Ecology and Evolutionary Biology, University of Arizona *Correspondence: [email protected] Morphological tree estimation We accounted for the substantial effect of lost Volvox section Volvox species (Volvox merrillii, Volvox amboensis, Volvox prolificus) on ancestral state reconstruction (Figure S3) by estimating a tree from morphological traits for the seven species with genetic data. If the morphological tree and genetic tree are congruent, morphology may be used to estimate species relationships. We used previously defined morphological traits (Nozaki and Itoh 1994) and traits which serve to morphologically identify species (Isaka et al. 2012), for a total of 39 traits. After removing invariant traits, seven traits remained (Table S3). Other traits (cell number, colony length, etc.) are not included as they are highly variable within species (Isaka et al. 2012; Nozaki et al. 2015). It was not possible to use PartitionFinder (Lanfear et al. 2016) to automatically identify the optimal model of evolution due to an insufficient number of traits; therefore, trees were estimated using both BINCAT and BINGAMMA models. Trees were made with RAxML version 8.0.20 (Stamatakis 2014) using 1,000 bootstraps. Both morphological trees had poor bootstrap support (below 32, Figure S4), and both trees were discordant with the chloroplast and ITS trees (normalized Robinson-Foulds distance, 50 to 75% of nodes different; Robinson & Foulds, 1981).
    [Show full text]