Tese 2014 Cristiane Gouvêa Fajardo
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CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya
CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya RENATO GOBBI VETTORAZZI UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO - UENF CAMPOS DOS GOYTACAZES - RJ FEVEREIRO - 2020 CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya RENATO GOBBI VETTORAZZI “Tese apresentada ao Centro de Ciências e Tecnologias Agropecuárias da Universidade Estadual do Norte Fluminense Darcy Ribeiro, como parte das exigências para obtenção do título de Doutor em Genética e Melhoramento de Plantas” Orientadora: Prof.ª Virginia Silva Carvalho CAMPOS DOS GOYTACAZES - RJ FEVEREIRO – 2020 FICHA CATALOGRÁFICA UENF - Bibliotecas Elaborada com os dados fornecidos pelo autor. V592 Vettorazzi, Renato Gobbi. Criopreservação de orquídeas brasileiras do gênero Cattleya / Renato Gobbi Vettorazzi. - Campos dos Goytacazes, RJ, 2020. 118 f. : il. Bibliografia: 80 - 93. Tese (Doutorado em Genética e Melhoramento de Plantas) - Universidade Estadual do Norte Fluminense Darcy Ribeiro, Centro de Ciências e Tecnologias Agropecuárias, 2020. Orientadora: Virginia Silva Carvalho. 1. Orchidaceae. 2. Banco Criogênico. 3. Vitrificação. 4. Manutenção da Diversidade. 5. Conservação de Germoplasma. I. Universidade Estadual do Norte Fluminense Darcy Ribeiro. II. Título. CDD - 631.5233 AGRADECIMENTOS A Deus, pela proteção, graças e bênçãos concedidas; À minha esposa Milena, por não medir esforços em ajudar, pelo companheirismo, carinho, paciência, compreensão e, sobretudo, pelo amor sempre presente; À minha família, pelas orações e por me fazer uma pessoa digna. Em especial, -
The Genus Brassavola, (L.) R.Br
The Genus Brassavola, (L.) R.Br. in W.T.Aiton, Hortus Kew. 5: 216 (1813) Type: Brassavola [B.] cucullata [bra-SAH-vo-la kyoo-kyoo-LAH-ta] There are 28 species (OrchidWiz [update Dec 2017]) that are epiphytes and sometimes lithophytes at elevations of from sea level to 3300 ft (1000 m) from Mexico, southern Caribbean islands to northern Argentina in moist or wet montane forests, mangroves, rocky crevices and cliff faces. They are most fragrant at night and many with a citrus smell. The genus is characterized by very small pencil-like pseudobulbs, often forming large clumps; a single, fleshy, apical, sub-terete leaf and the inflorescence produced form the apex of the pseudobulb. The inflorescence carries from a single to a few large flowers. The floral characteristics are elongate narrow similar sepals and petals, the base of the lip usually tightly rolled around at least a portion of the column which carries 12, sometimes eight unequal pollina with prominent opaque caudicles. The flowers usually occur, as a rule, in spring, summer and fall. The flowers are generally yellow to greenish white with a mostly white lip. It is not unusual for dark spots, usually purple, to be in the region where the sepals, petals, and lip join the stem (claw). This spotting is a dominant generic trait in Brassavola nodose. They are easily cultivated under intermediate conditions. Although this is a relatively small genus (28 species), the species show an unusually close relationship with one another in their floral patterns, coloration, and column structure making identification difficult, key to know where the plants were collected. -
New Cattleya Orchid Hybrid(1)
GUILHERME AUGUSTO CITO ALVES CORREIO et. al 145 CULTIVAR DESCRIPTION New Cattleya orchid hybrid(1) GUILHERME AUGUSTO CITO ALVES(2), RODRIGO THIBES HOSHINO(2), DOUGLAS JUNIOR BERTONCELLI(2), RONAN CARLOS COLOMBO(2), VANESSA STEGANI(3), RICARDO TADEU DE FARIA(2) ABSTRACT The hybrid, obtained by back crossing between (Cattleya labiata x Cattleya forbesii) x Cattleya labiata is a vigorous plant, bi- or unifoliate, features slender and cylindrical pseudobulbs and leathery dark-greenish leaves, with oblanceolate shape format of blunt tips with the first flowering four years after sowing. In Londrina, flowering occurred twice a year, between the months of April and May and October and November with 2-4 flowers per pseudobulb and durability ranging from 15 to 20 days. The flowers of the new hybrid were purple with a labellum with a yellow center and purple stripes. Keywords: breeding, floriculture, Orchidaceae, selection. RESUMO Novo híbrido de orquídea Cattleya O híbrido, obtido do retrocruzamento entre (Cattleya labiata x Cattleya forbesii) x Cattleya labiata é uma planta vigorosa, bi ou unifoliada, apresenta pseudobulbos delgados e cilíndricos e folhas coriáceas com tonalidade verde escuro, com formato oblanceolado, de pontas obtusas, apresentando o primeiro florescimento após quatros anos da semeadura. O florescimento em Londrina ocorreu duas vezes no ano, entre os meses de abril e maio e outubro e novembro com 2-4 flores por pseudobulbo e durabilidade entre 15 a 20 dias. As flores do novo híbrido possuem coloração lilás e labelo com um centro amarelo e listras roxas. Palavras-chave: floricultura, melhoramento, Orchidaceae, seleção. 1. INTRODUCTION allows the introduction of relevant genes such as shape, durability, color, and others into hybrids of orchids The Orchidaceae family has about 26,500 species, (WITHNER, 1988) distributed across all continents (KEW, 2011). -
Copyrighted Material
Index Page numbers in italics refer to Figures; those in bold to Tables. Abies 32 albuminous cells 42, 44, 65, 65, Acacia alata 81, 85, 98 108 Acer 164 alcian blue 182 Acer pseudoplatanus 165, 166 alcohol-based fi xatives 171–2 achenes 128 aleurone grains 102 acid bog habitat 152 algae 6 Acmopyle pancheri 65 Alismatales 67 acrolein 172–3 Allium 18, 19, 111 fi xation procedure 174–5 Alnus glutinosa 28, 29, 37, 165, 167 adaptations 6–8, 135–53 Alnus nepalensis 29 ecological 73, 76, 137–8 Aloe 9, 76, 77, 78, 139 hydrophytes 150–2 Aloe lateritia var. kitaliensis 77, 79 mechanical 135–7 Aloe somaliensis 140 mesophytes 147–50 aloes 13, 76, 78, 86, 142, 157 practical aspects 152–3 Ammophila 139, 142 xerophytes see xerophytes Ammophila arenaria (marram grass) Aegilops crassa 95, 99, 102 82, 92, 141 aerial roots 49, 149 Anacardiaceae 86, 139 Aerva lanata 81 Anarthria 156 Aesculus hippocastanum 129 Anarthriaceae 156, 156 Aesculus pavia 44 angiosperms 4, 7, 10 Agave 10, 76 fl oral part vascularization 121–3 Agave franzonsinii 95, 102 phloem 65, 108 Agrostis 100, 138COPYRIGHTEDsecondary MATERIAL 43–5 Agrostis stolonifera 99 taxonomy 155 Ailanthus 159 wood (secondary xylem) 31–6, air spaces 36 hydrophytes 150 axial system 33 mesophyll 74, 97, 112 growth rings 33, 35, 41 xerophytes 146 rays 35–6 Ajuga reptans var. atropurpurescens ring porous 33–4, 41 110 animal feeds 159–60 Albuca 73 animal pests 162–3 288 Annonaceae 130 black ironwood (Krugiodendron annuals 7, 8, 57 ferreum) 33 Anthemis 128 Boehmeria 62 Index Anthemis arvenis 128, 130 Bombax (kapok) -
Estudio De Factibilidad De Exportación De Orquídeas Ecuatorianas Utilizando La Estrategia B2c”
UNIVERSIDAD DE GUAYAQUIL FACULTAD DE CIENCIAS ECONÓMICAS MAESTRÍA EN NEGOCIOS INTERNACIONALES CON MENCION EN COMERCIO EXTERIOR TESIS PRESENTADA PARA OPTAR EL GRADO DE MAGÍSTER EN NEGOCIOS INTERNACIONALES CON MENCIÓN EN COMERCIO EXTERIOR “ESTUDIO DE FACTIBILIDAD DE EXPORTACIÓN DE ORQUÍDEAS ECUATORIANAS UTILIZANDO LA ESTRATEGIA B2C” ELABORADOR POR: TANIA PALACIOS SARMIENTO TUTOR DE TESIS: ING. MARIO VASQUEZ J. GUAYAQUIL – ECUADOR DICIEMBRE - 2015 1 DERECHOS DE AUTORÍA POR MEDIO DE LA PRESENTE CERTIFICO QUE LOS CONTENIDOS DESARROLLADOS EN ESTA TESIS SON DE ABSOLUTA PROPIEDAD Y RESPONSABILIDAD DE TANIA PALACIOS S. CON C.C. No. 0917542672, CUYO TEMA ES: “ESTUDIO DE FACTIBILIDAD DE EXPORTACIÓN DE ORQUÍDEAS ECUATORIANAS UTILIZANDO LA ESTRATEGIA B2C” TANIA PALACIOS S. C.C. No. 0917542672 GUAYAQUIL, DICIEMBRE DE 2015. 2 CERTIFICACIÓN DEL TUTOR ING. COM. MARIO VASQUEZ JIMENEZ, TUTOR DE LA TESIS PARA GRADO DENOMINADA: “ESTUDIO DE FACTIBILIDAD DE EXPORTACIÓN DE ORQUÍDEAS ECUATORIANAS UTILIZANDO LA ESTRATEGIA B2C” COMO REQUISITO PARA OPTAR POR EL TÍTULO DE MAGISTER EN NEGOCIOS INTERNACIONALES POR LA EGRESADA: TANIA PALACIOS S. C.C. No. 0917542672 CERTIFICA QUE: SE HA DESARROLLADO, REVISADO Y APROBADO EN TODAS SUS PARTES, POR CONSIGUIENTE SE ENCUENTRA APTA PARA SU TRÁMITE DE SUSTENTACIÓN. ______________________________________ Ing. Com. Mario Vásquez Jiménez TUTOR DE TESIS 3 AGRADECIMIENTO TANIA PALACIOS Agradezco a mi amiga Viviana Medina, mi compañera y amiga de estudios del pregrado en la ESPOL, ya que gracias a su intensa insistencia y tortura diaria me ayudó a encender motores para terminar este gran reto; el mismo que ha sido a base de mucho sacrificio. Y también agradezco a mi Dios, ya que me ha concedido vida y gracias a su voluntad puedo terminar este sueño que creí no lograrlo. -
Genetic Variation and Conservation of Changnienia Amoena, an Endangered Orchid Endemic to China
Pl. Syst. Evol. 258: 251–260 (2006) DOI 10.1007/s00606-006-0410-4 Genetic variation and conservation of Changnienia amoena, an endangered orchid endemic to China A. Li and S. Ge Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China Received November 4, 2005; accepted December 27, 2005 Published online: April 11, 2006 Ó Springer-Verlag 2006 Abstract. Changnienia amoena is a diploid and genetic structure found in C. amoena. Based on self-compatible orchid endemic to China. This these findings, we proposed conservation manage- species is in great danger of extinction with its ments for this endangered species, including current distribution being highly fragmented and habitat protection along with the protection of discontinuous. This study investigated the level their pollinators, artificial pollination as well as and apportionment of genetic diversity of this ex situ conservation. species using RAPD technique. Based on 119 discernible DNA fragments generated by 16 prim- Key words: Conservation genetics, RAPDs, ers, an intermediate level of genetic diversity was Changnienia amoena, endangered orchid. found at the species level with the percentage of polymorphic bands (P) of 76.5%, expected het- erozygosity (He) of 0.194. However, the genetic Introduction diversity at the population level was significantly lower (P=37.2%, He=0.120) compared with the The Orchidaceae is one of the largest and most average of other species with similar life history diverse families of flowering plants, including characteristics. A high level of population differ- up to one tenth of all flowering plant species in entiation was detected with 43.8% variation the world (Dressler 1993). -
Redalyc.DNA Barcode Regions for Differentiating Cattleya Walkeriana and C. Loddigesii
Acta Scientiarum. Biological Sciences ISSN: 1679-9283 [email protected] Universidade Estadual de Maringá Brasil Rivera-Jiménez, Hernando; Rossini, Bruno César; Vagner Tambarussi, Evandro; Veasey, Elizabeth Ann; Ibanes, Bruna; Marino, Celso Luis DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii Acta Scientiarum. Biological Sciences, vol. 39, núm. 1, enero-marzo, 2017, pp. 45-52 Universidade Estadual de Maringá Maringá, Brasil Available in: http://www.redalyc.org/articulo.oa?id=187150588007 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Acta Scientiarum http://www.uem.br/acta ISSN printed: 1679-9283 ISSN on-line: 1807-863X Doi: 10.4025/actascibiolsci.v39i1.33024 DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii Hernando Rivera-Jiménez1, Bruno César Rossini1,2, Evandro Vagner Tambarussi3,4*, Elizabeth Ann Veasey5, Bruna Ibanes5 and Celso Luis Marino1 1Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil. 2Instituto de Biotecnologia, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil. 3Universidade Estadual do Centro-Oeste, PR-153, Km 7, 84500-000, Irati, Paraná, Brazil. 4Programa de Pós-Graduação em Ciência Florestal, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Rua José Barbosa de Barros, 1780, 18610-307, Botucatu, São Paulo, Brazil. 5Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, São Paulo, Brazil. *Author for correspondence. E-mail: [email protected]. ABSTRACT. Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. -
Universidade Estadual De Mato Grosso Do Sul Unidade Universitária De Dourados Programa De Pós-Graduação Em Recursos Naturais
Universidade Estadual de Mato Grosso do Sul Unidade Universitária de Dourados Programa de Pós-Graduação em Recursos Naturais TÉCNICAS DE CULTIVO in vitro COMO ALTERNATIVA PARA A CONSERVAÇÃO DE Schomburgkia crispa Lindl. (ORCHIDACEAE) E SUA REINTRODUÇÃO EM AMBIENTE NATURAL Acadêmica: Jackeline Schultz Soares Dourados - MS Fevereiro de 2018 Universidade Estadual de Mato Grosso do Sul Unidade Universitária de Dourados Programa de Pós-Graduação em Recursos Naturais TÉCNICAS DE CULTIVO in vitro COMO ALTERNATIVA PARA A CONSERVAÇÃO DE Schomburgkia crispa Lindl. (ORCHIDACEAE) E SUA REINTRODUÇÃO EM AMBIENTE NATURAL Acadêmica: Jackeline Schultz Soares Orientador: Profº Dr. Etenaldo Felipe Santiago Coorientadora: Profª Drª Yara B. C. J. Rosa (in memoriam) “Tese apresentada ao programa de pós- graduação em Recursos Naturais, área de concentração em Recursos Naturais, da Universidade Estadual de Mato Grosso do Sul, como parte das exigências para a obtenção do título de Doutor em Recursos Naturais”. Dourados - MS Fevereiro de 2018 S654t Soares, Jackeline Schultz Técnicas de cultivo in vitro como alternativa para a conservação de Schomburgkia crispa Lindl.(Orchidceae) e sua reintrodução em ambiente natural / Jackeline Schultz Soares. Dourados, MS: UEMS, 2018. 101p. ; 30cm. Tese (Doutorado) – Recursos Naturais – Universidade Estadual de Mato Grosso do Sul, Unidade Universitária de Dourados, 2018. Orientador: Prof. Dr. Etenaldo Felipe Santiago. 1. Orchidaceae. 2. Semeadura assimbiótica. 3. Cerrado. I. Título. CDD 23.ed. 584.15 Cdd . - ????????? “E peço isto: que o vosso amor cresça mais e mais em ciência e em todo o conhecimento” Filipenses 1:9 iii A Deus, À minha família, À Profª. Drª. Yara Brito Chaim Jardim Rosa (in memoriam), por ter me acompanhado desde o início, me ensinando com maestria o ofício da pesquisa científica, Dedico. -
Sistemática Y Evolución De Encyclia Hook
·>- POSGRADO EN CIENCIAS ~ BIOLÓGICAS CICY ) Centro de Investigación Científica de Yucatán, A.C. Posgrado en Ciencias Biológicas SISTEMÁTICA Y EVOLUCIÓN DE ENCYCLIA HOOK. (ORCHIDACEAE: LAELIINAE), CON ÉNFASIS EN MEGAMÉXICO 111 Tesis que presenta CARLOS LUIS LEOPARDI VERDE En opción al título de DOCTOR EN CIENCIAS (Ciencias Biológicas: Opción Recursos Naturales) Mérida, Yucatán, México Abril 2014 ( 1 CENTRO DE INVESTIGACIÓN CIENTÍFICA DE YUCATÁN, A.C. POSGRADO EN CIENCIAS BIOLÓGICAS OSCJRA )0 f CENCIAS RECONOCIMIENTO S( JIOI ÚGIC A'- CICY Por medio de la presente, hago constar que el trabajo de tesis titulado "Sistemática y evo lución de Encyclia Hook. (Orchidaceae, Laeliinae), con énfasis en Megaméxico 111" fue realizado en los laboratorios de la Unidad de Recursos Naturales del Centro de Investiga ción Científica de Yucatán , A.C. bajo la dirección de los Drs. Germán Carnevali y Gustavo A. Romero, dentro de la opción Recursos Naturales, perteneciente al Programa de Pos grado en Ciencias Biológicas de este Centro. Atentamente, Coordinador de Docencia Centro de Investigación Científica de Yucatán, A.C. Mérida, Yucatán, México; a 26 de marzo de 2014 DECLARACIÓN DE PROPIEDAD Declaro que la información contenida en la sección de Materiales y Métodos Experimentales, los Resultados y Discusión de este documento, proviene de las actividades de experimen tación realizadas durante el período que se me asignó para desarrollar mi trabajo de tesis, en las Unidades y Laboratorios del Centro de Investigación Científica de Yucatán, A.C., y que a razón de lo anterior y en contraprestación de los servicios educativos o de apoyo que me fueron brindados, dicha información, en términos de la Ley Federal del Derecho de Autor y la Ley de la Propiedad Industrial, le pertenece patrimonialmente a dicho Centro de Investigación. -
CVIOS NEWSLETTER CENTRAL VANCOUVER ISLAND ORCHID SOCIETY November 2017
CVIOS NEWSLETTER CENTRAL VANCOUVER ISLAND ORCHID SOCIETY November 2017 CVIOS Meeting are held September to June on a Saturday at the Harewood Activity Center, 195 Fourth St, Nanaimo. UPCOMING MEETINGS Doors open at 11:00 with a brief business meeting starting & EVENTS at 12pm. Following is a display of plants brought in by members, a coffee break, prize draw and a featured 2017/2018: presentation or demonstration. There is a sales table where orchid supplies and plants brought in by members November 18: Visit to can be purchased Paramount Orchids in Parksville Mailing Address: PO Box 1061 December 9: Christmas Party Nanaimo BC, V9R 5Z2 Website: www.cvios.org Email: Laurie Forbes: [email protected] January 20: Meeting Newsletter Submissions: [email protected] February 17: Meeting & Bag Draw CVIOS EXECUTIVE 2017/18 March 17: Meeting President & AOS Chair: Laurie Forbes Past President: Bryan Emery April 21: Meeting Vice President: Constance Gordon-Webster Treasurer: Darlene Rathwell Secretary & Newsletter: Suzanne Currie May: 26: Plant Sale Membership: Dora Glover Plant Sales Table: Donna McDonnell June 16: Last Meeting until Library: Michael DeLeur September Refreshments: Sandra Lathrope Programs: Alexey Tretyakov Directors At Large: Sheila Wilson (publicity), Corey Timmins CVIOS NEWSLETTER CENTRAL VANCOUVER ISLAND ORCHID SOCIETY November 2017 CVIOS UPCOMING EVENTS: November 18 Paramount Orchid Visit – no regular meeting this month December 9 CVIOS Christmas party and potluck The Christmas party and potluck begins at 12pm. There will be a bag draw or silent auction – please bring items. Members please bring a dish to share, a plate and utensils. Members can also make an orchid display with their orchids and the winner will receive a prize. -
ORCHID POLLINATION ECOLOGY Orchid Pollination Exploring a Fascinating World by Ron Mchatton
ORCHID POLLINATION ECOLOGY Orchid Pollination Exploring a Fascinating World BY RON MCHATTON [1] The first example of sexual deception in Australian orchids involved the genus Cryptostylis. These orchids are pollinated by male parasitic wasps of the genus Lissopimpla. Here four male Lissopimpla excelsa wasps compete for the favors of a Cryptostylis erecta flower. MARK CLEMENTS [2] Silver-spotted skipper (Epargyreus clarus) pollinating a small purple fringed orchid (Platanthera psycodes) at Mt. Mitchell, North Carolina. The long spurs of the orchid have nectar at their ends and the skipper must push its proboscis deep into the spur to get to the nectar. Note the pollinia attached to the base of JAMES PETRANKA JAMES the skipper’s proboscis. bumblebee species. In this case, pollinia of Den. infundibulum are placed on the head of the bee while that from the Cymbidium, with much longer column, becomes at- tached to the bee’s central thorax. Because the column of the former species is much shorter, only pollinia placed near the front of the bee will be in a position to contact the stigmatic surface of the Dendrobium column as the bee exits the flower (Du Puy and Cribb 2007). Orchids with large gullet flowers such as Cymbidium are typically pollinated by large carpenter bees and bumblebees are known to pollinate Spiranthes and are implicated in the pollination of northern NT NT U U and some high-elevation species where other large bees are less common or active. 3 4 ERIC H ERIC H Some bees gather oils from flowers rather than nectar or pollen and many orchids [3–4] Sobralia [3] and Cattleya [4], two gen- NO ONE IS REALLY SURE HOW LONG have evolved to attract these pollinators. -
Interspecific Genetic Analysis of Orchids in Brazil Using
Plant Syst Evol (2014) 300:1825–1832 DOI 10.1007/s00606-014-1009-9 ORIGINAL ARTICLE Interspecific genetic analysis of orchids in Brazil using molecular markers Cristiane Gouveˆa Fajardo • Fa´bio de Almeida Vieira • Wagner Franco Molina Received: 4 November 2013 / Accepted: 2 February 2014 / Published online: 28 February 2014 Ó Springer-Verlag Wien 2014 Abstract Several species of Orchidaceae, one of the that unifoliate and bifoliolate species are genetically largest plant families, are considered endangered through- divergent. Additionally, PCA indicated a close relation out South America and legal protection policies are needed between C. granulosa and C. schofieldiana, a species con- so they can be preserved. Inter simple sequence repeats sidered to be a variety of C. granulosa by many researchers. (ISSRs) markers are a potential tool to be used in the phy- Thus, we conclude that ISSR genetic markers are effective logenetic reconstruction of closely related species. In this in detecting genetic differentiation among orchid species. study, we evaluate the polymorphic information content (PIC) and optimum number of ISSR markers (ONM) for Keywords Brassavola tuberculata Á Cattleya Á ISSR Á five Laeliinae orchids in order to assess genetic diversity. Genetic differentiation Á Laeliinae Á Orchidaceae The phylogenetic relationships between Cattleya granu- losa, an endangered Brazilian orchid, and four other native Brazilian species (Brassavola tuberculata, Cattleya bicolor, Introduction Cattleya labiata and Cattleya schofieldiana) were analyzed for genetic diversity and differentiation. The 11 selected With approximately 24,000 recognized species and about 800 primers generated 166 unambiguous loci (PIC = 0.354; genera, Orchidaceae is one of the largest plant families (World ONM = 156).