Methamphetamine-Induced Degeneration of Dopaminergic Neurons Involves Autophagy and Upregulation of Dopamine Synthesis
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The Dopamine Transporter: More Exciting Than Housekeeping
The Dopamine Transporter: More Exciting than Housekeeping Susan Ingram Washington State University Vancouver Dopaminergic Synapse Presynaptic cell MGluR 2 Na+ Cl- GIRK D2 DA DA D2 D1 a1 Postsynaptic cell Currents are activated at lower dopamine concentrations than are required for transport Ingram, et al., 2002 Low DA concentrations increase firing Ingram, et al., 2002 DAT-mediated chloride current is excitatory in cultured midbrain DA neurons DEPOLARIZATION HYPERPOLARIZATION Ingram, et al., 2002 Proteins that regulate intracellular Cl- Whole-cell patch clamp recordings from DA neurons Raclopride Sulpiride Prazosin TTX biocytin Prasad and Amara, 2001 Amphetamine activates a DAT-mediated current at low concentrations Cultures Slices Watts, Fyfe and Ingram, unpublished data. DA neurons make glutamatergic autapses in culture and amphetamine increases AMPA currents Ingram, et al., unpublished data mbYFPQS localizes to the membrane of cultured midbrain neurons Watts, Jimenez and Ingram, unpublished data. Amphetamine stimulates a dose-dependent change in mbYFPQS fluorescence in both soma and dendrites of DA neurons Watts and Ingram, unpublished data. KCC2 Expression is different in cultures and slices cultures slices TH KCC2 Jamieson and Ingram, unpublished data. Baculoviral Transfections Watts and Ingram, unpublished data. Summary • DAT-mediated chloride current may alter excitability of DA neurons and integration of synaptic activity. • The current may be activated selectively (relative to transport) by low DA and amphetamine concentrations suggesting a role in increasing release of DA. • The amphetamine-mediated current is dose-dependent in both cultures and slices of midbrain neurons but is inhibited at high amphetamine concentrations (20 µM). • The mbYFPQS is a sensitive tool to measure intracellular chloride concentrations (K50 = 30 mM) and is useful for monitoring changes in intracellular chloride concentrations in dendrites. -
(19) United States (12) Patent Application Publication (10) Pub
US 20130289061A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2013/0289061 A1 Bhide et al. (43) Pub. Date: Oct. 31, 2013 (54) METHODS AND COMPOSITIONS TO Publication Classi?cation PREVENT ADDICTION (51) Int. Cl. (71) Applicant: The General Hospital Corporation, A61K 31/485 (2006-01) Boston’ MA (Us) A61K 31/4458 (2006.01) (52) U.S. Cl. (72) Inventors: Pradeep G. Bhide; Peabody, MA (US); CPC """"" " A61K31/485 (201301); ‘4161223011? Jmm‘“ Zhu’ Ansm’ MA. (Us); USPC ......... .. 514/282; 514/317; 514/654; 514/618; Thomas J. Spencer; Carhsle; MA (US); 514/279 Joseph Biederman; Brookline; MA (Us) (57) ABSTRACT Disclosed herein is a method of reducing or preventing the development of aversion to a CNS stimulant in a subject (21) App1_ NO_; 13/924,815 comprising; administering a therapeutic amount of the neu rological stimulant and administering an antagonist of the kappa opioid receptor; to thereby reduce or prevent the devel - . opment of aversion to the CNS stimulant in the subject. Also (22) Flled' Jun‘ 24’ 2013 disclosed is a method of reducing or preventing the develop ment of addiction to a CNS stimulant in a subj ect; comprising; _ _ administering the CNS stimulant and administering a mu Related U‘s‘ Apphcatlon Data opioid receptor antagonist to thereby reduce or prevent the (63) Continuation of application NO 13/389,959, ?led on development of addiction to the CNS stimulant in the subject. Apt 27’ 2012’ ?led as application NO_ PCT/US2010/ Also disclosed are pharmaceutical compositions comprising 045486 on Aug' 13 2010' a central nervous system stimulant and an opioid receptor ’ antagonist. -
Program Book
PARKINSON’SDISEASE2014 ADVANCING RESEARCH, IMPROVING LIVES PROGRAM MATERIALS Sponsored by: January 6 – 7, 2014 Natcher Conference Center National Institutes of Health Bethesda, MD About our cover: The program cover image is a stylized version of the Parkinson’s Disease Motor-Related Pattern (PDRP), an abnormal pattern of regional brain function observed in MRI studies which shows increased metabolism indicated by red in some brain regions (pallidothalamic, pontine, and motor cortical areas), and decreased metabolism indicated by blue in others (associated lateral premotor and posterior parietal areas). Original image used with permission of David Eidelberg, M.D. For further information see: Hirano et al., Journal of Neuroscience 28 (16): 4201-4209. Welcome Message from Dr. Story C. Landis Welcome to the National Institute of Neurological Disorders and Stroke (NINDS) conference, “Parkinson’s Disease 2014: Advancing Research, Improving Lives.” Remarkable new discoveries and technological advances are rapidly changing the way we study the biological mechanisms of Parkinson’s disease, identify paths to improved treatments, and design effective clinical trials. Elucidating mechanisms and developing and testing effective interventions require a diverse set of approaches and perspectives. The NINDS has organized this conference with the primary goal of seeking consensus on, and prioritizing, research recommendations spanning clinical, translational, and basic Parkinson’s disease research that we support. We have assembled a stellar and dedicated group of session chairs and panelists who have worked collaboratively to identify emerging research opportunities in Parkinson’s research. While we have divided our working groups into three main research areas, we expect each will inform the others over the course of the next two days, and we look forward to both complementary and unique perspectives. -
Pharmacological Characterizations of H05, a Novel Potent Serotonin And
JPET/2018/248351 Title: Pharmacological characterization of (3-(benzo[d][1,3] dioxol-4-yloxy) -3-(4-fluorophenyl)-N, N-dimethylpropan-1-amine (H05), a novel serotonin and noradrenaline reuptake inhibitor with moderate 5-HT2A antagonist activity for the treatment of depression Authors: Xiangqing Xu, Yaqin Wei, Qiang Guo, Song Zhao, Zhiqiang Liu, Ting Xiao, Yani Liu, Yinli Qiu, Yuanyuan Hou, Guisen Zhang and KeWei Wang Affiliations: Department of Molecular and Cellular Pharmacology, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University , Beijing, China (X.X., T.X., K.W.W.); School of pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu, China (Y.W.); Institute of pharmaceutical research, Jiangsu Nhwa Pharmaceutical Co., Ltd., Xuzhou, Jiangsu, China (Q.G., S.Z., Z.L., Y.Q., Y.H., G.Z.); Department of Pharmacology, School of Pharmacy, Qingdao University, Qingdao, Shandong, China (Y.L., K.W.W.) 1 Running title page Running title: A novel SNRI with 5-HT2A antagonist activity for treatment of depression Corresponding author: KeWei Wang Address: No. 38 Xueyuan Road, Haidian District, Beijing, 100191, China. Tel: 86•10•82805605; E•mail: [email protected] or [email protected] Number of text pages: 57 Number of tables: 3 Number of figures: 7 Number of references: 71 Words in Abstract: 248 Words in Introduction: 712 Words in Discussion:1357 Abbreviations: ADs: antidepressants; SSRIs: selective serotonin reuptake inhibitors; NRIs: norepinephrine reuptake inhibitors; SNRIs: serotonin and norepinephrine -
Current Topics in Behavioral Neurosciences
Current Topics in Behavioral Neurosciences Series Editors Mark A. Geyer, La Jolla, CA, USA Bart A. Ellenbroek, Wellington, New Zealand Charles A. Marsden, Nottingham, UK For further volumes: http://www.springer.com/series/7854 About this Series Current Topics in Behavioral Neurosciences provides critical and comprehensive discussions of the most significant areas of behavioral neuroscience research, written by leading international authorities. Each volume offers an informative and contemporary account of its subject, making it an unrivalled reference source. Titles in this series are available in both print and electronic formats. With the development of new methodologies for brain imaging, genetic and genomic analyses, molecular engineering of mutant animals, novel routes for drug delivery, and sophisticated cross-species behavioral assessments, it is now possible to study behavior relevant to psychiatric and neurological diseases and disorders on the physiological level. The Behavioral Neurosciences series focuses on ‘‘translational medicine’’ and cutting-edge technologies. Preclinical and clinical trials for the development of new diagostics and therapeutics as well as prevention efforts are covered whenever possible. Cameron S. Carter • Jeffrey W. Dalley Editors Brain Imaging in Behavioral Neuroscience 123 Editors Cameron S. Carter Jeffrey W. Dalley Imaging Research Center Department of Experimental Psychology Center for Neuroscience University of Cambridge University of California at Davis Downing Site Sacramento, CA 95817 Cambridge CB2 3EB USA UK ISSN 1866-3370 ISSN 1866-3389 (electronic) ISBN 978-3-642-28710-7 ISBN 978-3-642-28711-4 (eBook) DOI 10.1007/978-3-642-28711-4 Springer Heidelberg New York Dordrecht London Library of Congress Control Number: 2012938202 Ó Springer-Verlag Berlin Heidelberg 2012 This work is subject to copyright. -
Compositions and Methods for Selective Delivery of Oligonucleotide Molecules to Specific Neuron Types
(19) TZZ ¥Z_T (11) EP 2 380 595 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 26.10.2011 Bulletin 2011/43 A61K 47/48 (2006.01) C12N 15/11 (2006.01) A61P 25/00 (2006.01) A61K 49/00 (2006.01) (2006.01) (21) Application number: 10382087.4 A61K 51/00 (22) Date of filing: 19.04.2010 (84) Designated Contracting States: • Alvarado Urbina, Gabriel AT BE BG CH CY CZ DE DK EE ES FI FR GB GR Nepean Ontario K2G 4Z1 (CA) HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL • Bortolozzi Biassoni, Analia Alejandra PT RO SE SI SK SM TR E-08036, Barcelona (ES) Designated Extension States: • Artigas Perez, Francesc AL BA ME RS E-08036, Barcelona (ES) • Vila Bover, Miquel (71) Applicant: Nlife Therapeutics S.L. 15006 La Coruna (ES) E-08035, Barcelona (ES) (72) Inventors: (74) Representative: ABG Patentes, S.L. • Montefeltro, Andrés Pablo Avenida de Burgos 16D E-08014, Barcelon (ES) Edificio Euromor 28036 Madrid (ES) (54) Compositions and methods for selective delivery of oligonucleotide molecules to specific neuron types (57) The invention provides a conjugate comprising nucleuc acid toi cell of interests and thus, for the treat- (i) a nucleic acid which is complementary to a target nu- ment of diseases which require a down-regulation of the cleic acid sequence and which expression prevents or protein encoded by the target nucleic acid as well as for reduces expression of the target nucleic acid and (ii) a the delivery of contrast agents to the cells for diagnostic selectivity agent which is capable of binding with high purposes. -
ALC Neuroprotection in Mitochondrial Dysfunction V2 Mar2013x
LÍDIA ALEXANDRA DOS SANTOS CUNHA ACETYL -L-CARNITINE NEUROPROTECTION IN MITOCHONDRIAL DYSFUNCTION Tese de Candidatura ao Grau de Doutor em Patologia e Genética Molecular, submetida ao Instituto de Ciências Biomédicas Abel Salazar Universidade do Porto Orientadora: Doutora Maria Teresa Burnay Summavielle Categoria: Investigador Auxiliar Afiliação: Instituto de Biologia Molecular e Celular DIRECTIVAS LEGAIS Nesta Tese foram apresentados os resultados contidos nos artigos publicados ou em vias de publicação seguidamente mencionados: Cunha L , Horvath I, Ferreira S, Lemos J, Costa P, Vieira D, Veres DS, Szigeti K, Summavielle T, Máthé D, Metello LF Preclinical imaging: an essential ally in biosciences and drug development (Submitted for publication) Cunha L , Bravo J, Fernandes S, Magalhães A, Costa P, Metello LF, Horvath I, Borges F, Szigeti K, Máthé D, Summavielle T Acetyl-L-Carnitine preconditioning in methamphetamine exposure leads to excessive glucose uptake impairing reference memory and deregulated mitochondrial membrane function (Submitted for publication) Summavielle T, Cunha L , Damiani D , Bravo J, Binienda Z, Koverech A , Virmani A (2011) Neuroprotective action of acetyl-L-carnitine on methamphetamine-induced dopamine release . American Journal of Neuroprotection and Neuroregeneration 3:1-7. Comunicações orais apresentadas em congressos internacionais: Cunha L , Bravo J, Gonçalves R, Rodrigues C, Rodrigues A, Metello LF, Summavielle T The role of mitochondria in acetyl-L-carnitine neuroprotective action European Association of Nuclear Medicine Annual Congress. October 2012. Birmingham, UK. Eur J Nucl Med Mol Imaging (2011) 38 (Suppl 2):S227 iii Apresentações sob a forma de poster em congressos internacionais: Cunha L , Bravo J, Costa P, Fernandes S, Oliveira M, Castro R, Metello LF, Summavielle T Acetyl-L-carnitine improves cell bioenergetics European Association of Nuclear Medicine Annual Congress. -
Phencyclidine: an Update
Phencyclidine: An Update U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES • Public Health Service • Alcohol, Drug Abuse and Mental Health Administration Phencyclidine: An Update Editor: Doris H. Clouet, Ph.D. Division of Preclinical Research National Institute on Drug Abuse and New York State Division of Substance Abuse Services NIDA Research Monograph 64 1986 DEPARTMENT OF HEALTH AND HUMAN SERVICES Public Health Service Alcohol, Drug Abuse, and Mental Health Administratlon National Institute on Drug Abuse 5600 Fishers Lane Rockville, Maryland 20657 For sale by the Superintendent of Documents, U.S. Government Printing Office Washington, DC 20402 NIDA Research Monographs are prepared by the research divisions of the National lnstitute on Drug Abuse and published by its Office of Science The primary objective of the series is to provide critical reviews of research problem areas and techniques, the content of state-of-the-art conferences, and integrative research reviews. its dual publication emphasis is rapid and targeted dissemination to the scientific and professional community. Editorial Advisors MARTIN W. ADLER, Ph.D. SIDNEY COHEN, M.D. Temple University School of Medicine Los Angeles, California Philadelphia, Pennsylvania SYDNEY ARCHER, Ph.D. MARY L. JACOBSON Rensselaer Polytechnic lnstitute National Federation of Parents for Troy, New York Drug Free Youth RICHARD E. BELLEVILLE, Ph.D. Omaha, Nebraska NB Associates, Health Sciences Rockville, Maryland REESE T. JONES, M.D. KARST J. BESTEMAN Langley Porter Neuropsychiatric lnstitute Alcohol and Drug Problems Association San Francisco, California of North America Washington, D.C. DENISE KANDEL, Ph.D GILBERT J. BOTV N, Ph.D. College of Physicians and Surgeons of Cornell University Medical College Columbia University New York, New York New York, New York JOSEPH V. -
Terminoloogia Raamat.Pdf
A I G O O L O N I M R E T A I S T A A M R A F FARMAATSIATERMINOLOOGIA Euroopa farmakopöa ravimvormid, manustamisviisid, pakendid ja droogid ATC süsteemid FARMAATSIATERMINOLOOGIA Euroopa farmakopöa ravimvormid, manustamisviisid, pakendid ja droogid ATC süsteemid Tartu 2010 Koostajad: Toivo Hinrikus, Mailis Jaamaste, Eveli Kikas, Peet-Henn Kingisepp, Ott Laius, Ain Raal, Sander Sepp, Ave-Ly Toomvap, Peep Veski, Daisy Volmer Farmaatsiaterminoloogia ekspertkomisjoni liikmed aastast 1999 (Tartu Ülikool): Toivo Hinrikus, Peet-Henn Kingisepp, Ain Raal, Peep Veski, Daisy Volmer Farmaatsiaterminoloogia ekspertkomisjoni liikmed aastatel 1999–2009 (Ravimiamet): Birgit Aasmäe, Malle Jaagola, Mailis Jaamaste, Eveli Kikas, Silja Kokk, Signe Leito, Margit Plakso, Lembit Rägo, Helga Suija, Ave-Ly Toomvap, Merje Veeberg Keeletoimetaja: Rutt Läänemets Kaanefoto: Ain Raal Kirjastanud: Ravimiamet Nooruse 1, 50411 Tartu Telefon: +372 737 4140 Faks: +372 737 4142 E-post: [email protected] Trükise väljaandmist toetasid Eesti Terminoloogia Ühing ja Haridus- ja Teadusministeerium projekti „Terminikomisjonide toetuskonkurss 2009“ raames. Väljaande refereerimisel või tsiteerimisel palume viidata allikale. ISBN 978-9949-18-919-9 Saateks Räägime ravimitest eesti keeles Ravimimaailmas toimub üleilmastumine tohutu hooga. Sageli mõeldakse ravim välja ühes, selle omadusi ja toimet hinnatakse teises ning seda toodetakse kolmandas maailma nurgas. Sama ravimit võivad kasutada arstid, apteekrid ja patsiendid üle kogu maailma. Kui ravimi kõrvaltoime registreeritakse näiteks Portugalis, peame meiegi Eestis sellest oma järel- dused tegema ja teadma, mis on sellest oluline meie patsientide jaoks. Selleks, et ravimitega tegelejad erinevates riikides üksteist mõistaksid, peavad nad kasutama ühtset terminoloogiat. Nüüdisajal on üldkasutatavaks suhtlemisvahendiks teatavasti inglise keel. Just inglise keeles trükitud Euroopa farmakopöa on aluseks ravimitega seonduvate terminite kasuta- misele kogu Euroopas. -
G-33-667 Cost Share
11:40:18 OCA PAD INITIATION - PROJECT HEADER INFORMATION 12/13/89 Active Project #: G-33-667 Cost share #: Rev #: 0 Center # : 10/24-6-R6834-1A0 Center shr #: OCA file #: Work type : RES Contract#: 1 RO1 DA06305-01 Mod #: Document : GRANT Prime #: Contract entity: GTRC Subprojects ? : N Main project #: Project unit: CHEM Unit code: 02.010.136 Project director(s): ZALKOW L H CHEM (404)894-4045 Sponsor/division names: DHHS/PHS/ADAMHA / ALCOHOL, DRUG ABUSE & MENTAL Sponsor/division codes: 108 / 004 Award period: 890930 to 900831 (performance) 901130 (reports) Sponsor amount New this change Total to date Contract value 187,296.00 187,296.00 Funded 187,296.00 187,296.00 Cost sharing amount 0.00 Does subcontracting plan apply ?: N Title: IRREVERSIBLE ANTAGONISTS OF COCAINE AND OTHER STIMULANTS PROJECT ADMINISTRATION DATA OCA contact: Kathleen R. Ehlinger 894-4820 Sponsor technical contact Sponsor issuing office KIM PHAM, PH.D. CATHERINE MILLS (301)443-5280 (301)443-6024 NATIONAL INSTITUTE ON DRUG ABUSE NATIONAL INSTITUTE ON DRUG ABUSE 5600 FISHERS LANE 5600 FISHERS LANE, ROOM 10-25 ROCKVILLE, MD. 20857 ROCKVILLE, MD. 20857 Security class (U,C,S,TS) : U ONR resident rep. is ACO (Y/N): N Defense priority rating : N/A N1H supplemental sheet Equipment title vests with: Sponsor GIT X Administrative comments - INITIATION OF PROJECT. YEAR 1 OF PROPOSED 3 YEAR PROJECT. GEORGIA INSTITUTE OF TECHNOLOGY OFFICE OF CONTRACT ADMINISTRATION NOTICE OF PROJECT CLOSEOUT Cloaeout Notice Date 03/19/91 Project No. G-33-667 Center No. 10/24-6-R6834-1A0_ Project Director ZALKOW L H School/Lab CHEMISTRY Sponsor DHHS/PHS/ADAMHA/ALCOHOL, DRUG ABUSE & MENTAL Contract/Grant No. -
Isoflurane Inhibits Dopaminergic Synaptic Vesicle Exocytosis Coupled to Cav2.1 and Cav2.2 in Rat Midbrain Neurons
This Accepted Manuscript has not been copyedited and formatted. The final version may differ from this version. Research Article: New Research | Neuronal Excitability Isoflurane inhibits dopaminergic synaptic vesicle exocytosis coupled to CaV2.1 and CaV2.2 in rat midbrain neurons Christina L. Torturo1,2, Zhen-Yu Zhou1, Timothy A. Ryan1,3 and Hugh C. Hemmings1,2 1Departments of Anesthesiology, Weill Cornell Medicine, New York, NY 10065 2Pharmacology, Weill Cornell Medicine, New York, NY 10065 3Biochemistry, Weill Cornell Medicine, New York, NY 10065 https://doi.org/10.1523/ENEURO.0278-18.2018 Received: 16 July 2018 Revised: 18 December 2018 Accepted: 21 December 2018 Published: 10 January 2019 Author Contributions: CLT, ZZ, TAR and HCH designed the research; CLT performed the research, TAR contributed unpublished reagents/analytic tools; CLT and ZZ analyzed the data; CLT, ZZ, TAR, and HCH wrote the paper. Funding: http://doi.org/10.13039/100000002HHS | National Institutes of Health (NIH) GM58055 Conflict of Interest: HCH: Editor-in-Chief of the British Journal of Anaesthesia; consultant for Elsevier. Funding Sources: NIH GM58055 Corresponding author: Hugh C. Hemmings, E-mail: [email protected] Cite as: eNeuro 2019; 10.1523/ENEURO.0278-18.2018 Alerts: Sign up at www.eneuro.org/alerts to receive customized email alerts when the fully formatted version of this article is published. Accepted manuscripts are peer-reviewed but have not been through the copyediting, formatting, or proofreading process. Copyright © 2019 Torturo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. -
Conference Schedule
Society on NeuroImmune Pharmacology (SNIP) 23rd Scientific Conference Hotel DOUBLETREE BY HILTON PHILADELPHIA CENTER CITY Philadelphia, PA, USA March 29 - April 1, 2017 Previous Conferences:1993 Toronto Hilton, Canada; 1994 Breakers, Palm Beach, FL; 1995 Bristol Court, San Diego, CA; 1996 Caribe Hilton, San Juan, PR; 1997 Opryland Hotel, Nashville TN; 1998 Scottsdale Princess, Scottsdale, AR; 2000 NIH Mazur Auditorium, Bethesda MD; 2001 Emory University, Atlanta, GA; 2002 Clearwater Beach Hilton, Clear Water, FL; 2004, La Fonda Hotel, Santa Fe, NM; 2005 Clearwater Beach Hilton, Clear Water, FL; 2006 La Fonda Hotel, Santa Fe, NM; 2007 City Center Marriott Hotel, Salt Lake City, UT; 2008 Francis Marion Hotel, Charleston, SC; 2009 Pearl Plaza Howard Johnson, Wuhan, China; 2010 Manhattan Beach Marriott, Manhattan Beach, CA; 2011 Hilton Clearwater Beach Resort, Clear Water Beach, FL; 2012 Hawaii Prince Hotel, Honolulu, HI; 2013 Conrad Hilton, San Juan, PR; 2014 Intercontinental New Orleans, LA.; 2015; Hyatt Regency, Miami, FL; 2016, Hotel Galaxy, Krakow, Poland. Page 1 TABLE OF CONTENTS Title Page 1 Table of Contents 2 Acknowledgement of Special Contributors and Sponsors 3-5 The SNIP Council, Officials, and Committees 6-7 Annual Society Awards 8-9 2016 Early Career Investigator Travel Awardees 9-11 2016 Plenary Speaker Biographies 12-14 Conference Agenda Wednesday March 29, 2017 City-Wide NeuroAIDS Discussion Group 15-16 Opening Reception 16 Poster Session 1 (W1-72, Abstract listings page 25) 16 1st Annual DISC Networking Hour 16 Thursday March 30, 2017 Presidential Symposium: Dopamine Neurotransmission in HIV-1 Infection 16-17 Presidential Symposium: Dr. David Sulzer 17 Symposium 2: Novel mechanisms of CNS infection 17-18 Meet the Mentors Lunch 18 SNIP Council Meeting 18 Pharmacology Symposium: Dr.