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Identification of Molecules Relevant for the Invasiveness of Fibrosarcomas and Melanomas
Helsinki University Biomedical Dissertations No. 148 IDENTIFICATION OF MOLECULES RELEVANT FOR THE INVASIVENESS OF FIBROSARCOMAS AND MELANOMAS Pirjo Nummela Department of Pathology Haartman Institute Faculty of Medicine and Division of Biochemistry Department of Biosciences Faculty of Biological and Environmental Sciences University of Helsinki Finland Academic dissertation To be presented for public examination with the permission of the Faculty of Biological and Environmental Sciences of the University of Helsinki in the Lecture Hall 2 of Haartman Institute (Haartmaninkatu 3, Helsinki), on 13.5.2011 at 12 o’clock noon. Helsinki 2011 Supervisor Docent Erkki Hölttä, M.D., Ph.D. Department of Pathology Haartman Institute University of Helsinki Thesis committee Docent Jouko Lohi, M.D., Ph.D. Department of Pathology Haartman Institute University of Helsinki and Pirjo Nikula-Ijäs, Ph.D. Division of Biochemistry Department of Biosciences University of Helsinki Reviewers Professor Veli-Matti Kähäri, M.D., Ph.D. Department of Dermatology University of Turku and Turku University Hospital and Docent Jouko Lohi, M.D., Ph.D. Opponent Professor Jyrki Heino, M.D., Ph.D. Department of Biochemistry and Food Chemistry University of Turku Custos Professor Kari Keinänen, Ph.D. Division of Biochemistry Department of Biosciences University of Helsinki ISBN 978-952-92-8821-2 (paperback) ISBN 978-952-10-6924-6 (PDF) ISSN 1457-8433 http://ethesis.helsinki.fi Helsinki University Print Helsinki 2011 To Juha, Joona, and Joel TABLE OF CONTENTS LIST OF ORIGINAL PUBLICATIONS -
Targeting the Phosphatidylinositide-3 Kinase Pathway and the Mitogen
Targeting the Phosphatidylinositide-3 Kinase Pathway and the Mitogen-Activated-Protein Kinase Pathway through Thymosin-β4, Exercise, and Negative Regulators to Promote Retinal Ganglion Cell Survival or Regeneration by Mark Magharious A thesis submitted in conformity with the requirements for the degree of Master of Science Rehabilitation Sciences Institute University of Toronto © Copyright by Mark Magharious 2015 Targeting the Phosphatidylinositide-3 Kinase Pathway and the Mitogen-Activated-Protein Kinase Pathway through Thymosin-β4, Exercise, and Negative Regulators to Promote Retinal Ganglion Cell Survival or Regeneration Mark Magharious Master of Science Rehabilitation Sciences Institute University of Toronto 2015 Abstract The phosphatidylinositide-3 kinase (PI3K) and mitogen-activated-protein kinase (MAPK) pathways mediate cellular survival in the presence of apoptotic stimuli. These pathways are known to promote the survival of injured retinal ganglion cells (RGCs), central nervous system neurons that project visual information from the retina to the brain. Injury to the optic nerve triggers apoptosis of RGCs. This work demonstrates that Thymosin-β4, a peptide involved in actin sequestration, both enhances RGC survival after injury and increases axonal regeneration. Moreover, Thymosin-β4 modulates the PI3K and MAPK pathways. In addition, this study demonstrates that exercise reduces apoptosis of injured RGCs, and explores the function of the PI3K and MAPK pathways in this process. Finally, small peptides are used to interfere with the functions of PTEN, a negative regulator of the PI3K pathway, as well as Erbin and BCR, negative regulators in the MAPK pathway. These peptides enhance RGC survival and axonal regeneration after injury. ii Acknowledgments I would like to take this opportunity to recognize all those who helped me through the process of researching and writing this thesis. -
Sized Neuropeptides
M ETHODS IN MOLECULAR BIOLOGY™ Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Neuropeptides Methods and Protocols Edited by Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria, Università degli Studi di Torino, Grugliasco, TO, Italy; Istituto Nazionale di Neuroscienze (INN), Università degli Studi di Torino, Grugliasco, TO, Italy Editor Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria Università degli Studi di Torino and Istituto Nazionale di Neuroscienze (INN) Università degli Studi di Torino Grugliasco, TO, Italy [email protected] Please note that additional material for this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-309-7 e-ISBN 978-1-61779-310-3 DOI 10.1007/978-1-61779-310-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011936011 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. -
Deer Thymosin Beta 10 Functions As a Novel Factor for Angiogenesis And
Zhang et al. Stem Cell Research & Therapy (2018) 9:166 https://doi.org/10.1186/s13287-018-0917-y RESEARCH Open Access Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration Wei Zhang1,2†, Wenhui Chu1,2†, Qingxiu Liu1,2†, Dawn Coates3, Yudong Shang1,2 and Chunyi Li1,2* Abstract Background: Deer antlers are the only known mammalian organ with vascularized cartilage that can completely regenerate. Antlers are of real significance as a model of mammalian stem cell-based regeneration with particular relevance to the fields of chondrogenesis, angiogenesis, and regenerative medicine. Recent research found that thymosin beta 10 (TMSB10) is highly expressed in the growth centers of growing antlers. The present study reports here the expression, functions, and molecular interactions of deer TMSB10. Methods: The TMSB10 expression level in both tissue and cells in the antler growth center was measured. The effects of both exogenous (synthetic protein) and endogenous deer TMSB10 (lentivirus-based overexpression) on antlerogenic periosteal cells (APCs; nonactivated antler stem cells with no basal expression of TMSB10) and human umbilical vein endothelial cells (HUVECs; endothelial cells with no basal expression of TMSB10) were evaluated to determine whether TMSB10 functions on chondrogenesis and angiogenesis. Differences in deer and human TMSB10 in angiogenesis and molecular structure were determined using animal models and molecular dynamics simulation, respectively. The molecular mechanisms underlying deer TMSB10 in promoting angiogenesis were also explored. Results: Deer TMSB10 was identified as a novel proangiogenic factor both in vitro and in vivo. Immunohistochemistry revealed that TMSB10 was widely expressed in the antler growth center in situ, with the highest expression in the reserve mesenchyme, precartilage, and transitional zones. -
Snomed Ct Dicom Subset of January 2017 Release of Snomed Ct International Edition
SNOMED CT DICOM SUBSET OF JANUARY 2017 RELEASE OF SNOMED CT INTERNATIONAL EDITION EXHIBIT A: SNOMED CT DICOM SUBSET VERSION 1. -
Echinoderm Antimicrobial Peptides to Contrast Human Pathogens
s Chemis ct try u d & Schillaci and Arizza, Nat Prod Chem Res 2013, 1:2 o r R P e s l e a r a r u DOI: 10.4172/2329-6836.1000109 t c h a N Natural Products Chemistry & Research ISSN: 2329-6836 Review article Open Access Echinoderm Antimicrobial Peptides to Contrast Human Pathogens Domenico Schillaci1* and Vincenzo Arizza1,2 1Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi 18, - 90123 Palermo, Ital 2Istituto Euromediterraneo di Scienza e Biotecnologia - 90139 Palermo, Italy Abstract Increased attention has been focused in marine invertebrates as a source of bioactive molecules for biomedical applications. Many bioactive molecules are part of the innate immune system. Some more recently isolated compounds, mainly from sea urchin and sea cucumber are antimicrobial peptides (AMP) active against Gram positive, Gram negative and fungi. In this review we described the most recent studies on AMP isolated from echinoderms. The AMP are little peptides <10 kDa with cationic charge and amphipathic structure. Recently, it was demonstrated that in the coelomocyte lysates of Paracentrotus. lividus and Holothuria tubulosa AMP are present with activity against staphylococcal and Pseudomonas aeruginosa antibiofilm. The data show the great potential of application of AMPs in biotechnology for developing novel therapeutic agents and as complements to conventional antibiotic therapy to combat the multi - resistant bacterial strains. Keywords: Antimicrobial peptides; Therapeutic agents; Antibiotic nucleic acids and/or enzymatic proteins, leading to bacterial cell death therapy [10-14]. Moreover, it appears that many AMPs may be multifunctional microbicides, acting simultaneously at the cell membrane and internal Introduction sites [11]. -
Catalogcatalog
CATALOGCATALOG 25052505 ParviewParview RoadRoad •• Middleton,Middleton, WIWI 53562-257953562-2579 •• 800-383-7799800-383-7799 www.newcomersupply.comwww.newcomersupply.com 20212021 ABOUT US IHC Mission Keeping it real: Our job is to help you get your job done! Everything we do is designed with your needs in mind. Our goal is to make sure your goals are met. Keeping the lines of communication open: Our technical support staff is ready to help you solve problems, resolve concerns, and make sure that your procedures run as smoothly as possible. Keeping prices competitive: We take pride in making sure our products arrive at your lab with the fairest price possible. Keeping a sense of humor: Life is Good! Don’t forget that. We won’t. If you’re having a bad day, don’t worry, it’s our joy to make sure your day is full of HISTOCALIFRAGILISTICEXPIALIDOCIOUS! We work hard to bring you the best histology supply services available. Please call 800-383-7799 or e-mail [email protected] anytime with any questions or comments. 1 ® 2505 Parview Road, Middleton, WI 53562-2579 • 800-383-7799 • www.newcomersupply.com 78 ORDERING INFORMATION PLACING AN ORDER RETURNS To place an order, contact customer service by phone, fax, Contact Newcomer Supply prior to returning any product. mail or email. Our experienced staff is available and ready If you have a problem with a product or shipment, call us to help you find the histology laboratory products you need. within five business days of the issue. Returns without a To see our current product listings, please visit our website. -
Luxol Fast Blue Protocol Ihc World
Luxol Fast Blue Protocol Ihc World RedfordIs Len shadowed absterging when distractedly Zacharias if singling euhemerizing Clyde loudensdespotically? or burgeons. Fractional Barnard pre-empt very distressfully while Bartlet remains spick and undespairing. HE staining of PFA fixed, frozen postmortem human brain tissue. Gmri trichrome stain Luxol fast red stain Methyl blue Movat's stain. Members have heavy walls in tap water. Product Description IHC World control slides are prepared using top quality tissues that ever been processed embedded and sectioned following standard. Ministerial action group on time would have likewise banded together. White glass, with ground edges. SVF offers advantages of direct with rapid isolation procedure to a xenobiotic-free environment. A fast music-resolution single-point 3D MPF mapping protocol was. MS is an autoimmune disease that causes demyelination of the neurons in the central nervous system. The world by two high on request is better tissue is made at sakura finetek usa, but we can easily. The presence of a tumor and its distance from the surgical margin is an essential element of tumor assessment. For Researchers Icahn School wilderness Medicine. Alternatively, the devise may be embedded separately, in specific ways, such equation the valves may be viewed optimally, or embedded with the apex first, poll that sections will play both ventricles for evaluation of thickness etc. Please support our research. Made of acetal polymer. The agreement tended to be better for FNACs than fluid specimens. These cassettes are used luxol fast blue, all protocols were approved. HT 09 Nerve Flashcards Quizlet. CNS by matrine in experimental autoimmune encephalomyelitis. -
Views of the NIDA, NINDS Or the National Summed Across the Three Auditory Forebrain Lobule Sec- Institutes of Health
Xie et al. BMC Biology 2010, 8:28 http://www.biomedcentral.com/1741-7007/8/28 RESEARCH ARTICLE Open Access The zebra finch neuropeptidome: prediction, detection and expression Fang Xie1, Sarah E London2,6, Bruce R Southey1,3, Suresh P Annangudi1,6, Andinet Amare1, Sandra L Rodriguez-Zas2,3,5, David F Clayton2,4,5,6, Jonathan V Sweedler1,2,5,6* Abstract Background: Among songbirds, the zebra finch (Taeniopygia guttata) is an excellent model system for investigating the neural mechanisms underlying complex behaviours such as vocal communication, learning and social interactions. Neuropeptides and peptide hormones are cell-to-cell signalling molecules known to mediate similar behaviours in other animals. However, in the zebra finch, this information is limited. With the newly-released zebra finch genome as a foundation, we combined bioinformatics, mass-spectrometry (MS)-enabled peptidomics and molecular techniques to identify the complete suite of neuropeptide prohormones and final peptide products and their distributions. Results: Complementary bioinformatic resources were integrated to survey the zebra finch genome, identifying 70 putative prohormones. Ninety peptides derived from 24 predicted prohormones were characterized using several MS platforms; tandem MS confirmed a majority of the sequences. Most of the peptides described here were not known in the zebra finch or other avian species, although homologous prohormones exist in the chicken genome. Among the zebra finch peptides discovered were several unique vasoactive intestinal and adenylate cyclase activating polypeptide 1 peptides created by cleavage at sites previously unreported in mammalian prohormones. MS-based profiling of brain areas required for singing detected 13 peptides within one brain nucleus, HVC; in situ hybridization detected 13 of the 15 prohormone genes examined within at least one major song control nucleus. -
FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen 2003
Boeken zonder publiciteit FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen NATIONAAL FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Interuniversitair Instituut voor Kernwetenschappen Fonds voor Geneeskundig Wetenschappelijk Onderzoek 2003 Lijst der kredietgenieters (Met opgave van hun onderzoeksprogramma en onthaalinstelling) Egmontstraat 5 1000 BRUSSEL Tel. (02) 512.91.10 With English Summary and Abstracts FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen NATIONAAL FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Interuniversitair Instituut voor Kernwetenschappen Fonds voor Geneeskundig Wetenschappelijk Onderzoek 2003 Lijst der kredietgenieters (Met opgave van hun onderzoeksprogramma en onthaalinstelling) Egmontstraat 5 1000 BRUSSEL Tel. (02) 512.91.10 AFKORTINGEN A.R.A. : Algemeen Rijksarchief en K.M.I. : Koninklijk Meteorologisch Rijksarchief in de Provinciën Instituut van België B.I.R.A. : Belgisch Instituut voor K.M.K.G. : Koninklijke Musea voor Ruimte-Aëronomie Kunst en Geschiedenis B.R. : Bibliothèque Royale Albert K.M.M.A. : Koninklijk Museum voor Ier Midden-Afrika C.E.G.E.S. : Centre d’Etudes et de Docu- K.M.S. : Koninklijke Militaire mentation Guerre et Sociétés School contemporaines K.M.S.K.B. : Koninklijke Musea voor C.L.O.Gent: Centrum voor Landbouwkun- Schone Kunsten van België dig Onderzoek - Gent K.S.B. : Koninklijke Sterrenwacht C.O.D.A. : Centrum voor Onderzoek in van België Diergeneeskunde en Agro- K.U.Brussel: Katholieke Universiteit chemie Brussel E.R.M. : Ecole Royale Militaire K.U.Leuven : Katholieke Universiteit E.S.K.E. : Egyptologische Stichting Leuven Koningin Elisabeth L.U.C. : Limburgs Universitair Cen- F.N.R.S. Fonds National de la Recher- trum che Scientifique M.R.A.C. : Musée Royal de l'Afrique F.P.Ms : Faculté Polytechnique de Centrale Mons N.F.W.O. -
\\Win1\Jp\Rapport 2004\Mand2004.3.Wpd
FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen NATIONAAL FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Interuniversitair Instituut voor Kernwetenschappen Fonds voor Geneeskundig Wetenschappelijk Onderzoek 2004 Lijst der kredietgenieters Egmontstraat 5 Boeken zonder publiciteit 1000 BRUSSEL Tel. (02) 512.91.10 FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen NATIONAAL FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Interuniversitair Instituut voor Kernwetenschappen Fonds voor Geneeskundig Wetenschappelijk Onderzoek 2004 Lijst der kredietgenieters Egmontstraat 5 With English Abstracts 1000 BRUSSEL Tel. (02) 512.91.10 AFKORTINGEN A.R.A. : Algemeen Rijksarchief en Rijksarchief in de Provinciën B.I.R.A. : Belgisch Instituut voor Ruimte-Aëronomie B.R. : Bibliothèque Royale Albert Ier C.E.G.E.S. : Centre d’Etudes et de Documentation Guerre et Sociétés contemporaines C.L.O.Gent : Centrum voor Landbouwkundig Onderzoek - Gent C.O.D.A. : Centrum voor Onderzoek in Diergeneeskunde en Agrochemie E.R.M. : Ecole Royale Militaire E.S.K.E. : Egyptologische Stichting Koningin Elisabeth FNRS : Fonds National de la Recherche Scientifique F.P.Ms : Faculté Polytechnique de Mons F.U.C.A.M. : Faculté Universitaire Catholique de Mons F.U.S.A.Gx : Faculté Universitaire des Sciences Agronomiques de Gembloux F.U.N.D.P. : Facultés Universitaires Notre-Dame de la Paix à Namur F.U.S.L. : Facultés Universitaires Saint-Louis à Bruxelles FWO : Fonds voor Wetenschappelijk Onderzoek - Vlaanderen I.A.P. : Instituut voor het Archeologisch Patrimonium I.B.W. : Instituut voor Bosbouw en Wildbeheer IIKW : Interuniversitair Instituut voor Kernwetenschappen I.M.E.C. : Interuniversitair Micro-elektronica Centrum v.z.w. I.N. : Instituut voor Natuurbehoud I.P.B. : Institu(u)t Pasteur van/du Brussel/Bruxelles I.R.M. -
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