Deer Thymosin Beta 10 Functions As a Novel Factor for Angiogenesis And
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Identification of Molecules Relevant for the Invasiveness of Fibrosarcomas and Melanomas
Helsinki University Biomedical Dissertations No. 148 IDENTIFICATION OF MOLECULES RELEVANT FOR THE INVASIVENESS OF FIBROSARCOMAS AND MELANOMAS Pirjo Nummela Department of Pathology Haartman Institute Faculty of Medicine and Division of Biochemistry Department of Biosciences Faculty of Biological and Environmental Sciences University of Helsinki Finland Academic dissertation To be presented for public examination with the permission of the Faculty of Biological and Environmental Sciences of the University of Helsinki in the Lecture Hall 2 of Haartman Institute (Haartmaninkatu 3, Helsinki), on 13.5.2011 at 12 o’clock noon. Helsinki 2011 Supervisor Docent Erkki Hölttä, M.D., Ph.D. Department of Pathology Haartman Institute University of Helsinki Thesis committee Docent Jouko Lohi, M.D., Ph.D. Department of Pathology Haartman Institute University of Helsinki and Pirjo Nikula-Ijäs, Ph.D. Division of Biochemistry Department of Biosciences University of Helsinki Reviewers Professor Veli-Matti Kähäri, M.D., Ph.D. Department of Dermatology University of Turku and Turku University Hospital and Docent Jouko Lohi, M.D., Ph.D. Opponent Professor Jyrki Heino, M.D., Ph.D. Department of Biochemistry and Food Chemistry University of Turku Custos Professor Kari Keinänen, Ph.D. Division of Biochemistry Department of Biosciences University of Helsinki ISBN 978-952-92-8821-2 (paperback) ISBN 978-952-10-6924-6 (PDF) ISSN 1457-8433 http://ethesis.helsinki.fi Helsinki University Print Helsinki 2011 To Juha, Joona, and Joel TABLE OF CONTENTS LIST OF ORIGINAL PUBLICATIONS -
The Future of Dermatopathology
Modern Pathology (2006) 19, S155–S163 & 2006 USCAP, Inc All rights reserved 0893-3952/06 $30.00 www.modernpathology.org The future of dermatopathology A Neil Crowson Departments of Dermatology, Pathology, and Surgery, University of Oklahoma and Regional Medical Laboratory, St John Medical Center, Tulsa, OK, USA Of the major issues that dermatopathology will face in the immediate future, two powerful challenges loom large. The first is the application of novel nondestructive imaging technologies to in vivo diagnosis in humans. The second is the application of molecular technologies to a diagnostic arena which formerly belonged exclusively to the light microscopist. The first to be considered in this context is the application of near infrared spectroscopy to the noninvasive in vivo diagnosis of neoplastic skin disease. The second will be a discussion of application, methodology and the current state of the art in microarray technologies as they apply to neoplastic dermatopathology and, in particular, the diagnosis and prognostication of melanoma. Modern Pathology (2006) 19, S155–S163. doi:10.1038/modpathol.3800513 Keywords: in vivo microscope; tissue microarray; basal cell carcinoma; infrared spectroscopy Noninvasive assessment of skin lesions keratoses and squamous cell carcinomata in vitro. by near infrared (IR) spectroscopy Melanocytic nevi could be subdivided into banal vs dysplastic nevi based upon their spectral differences In the late 1990s, working with Dr Laura McIntosh and melanomas could be separately recognized as and colleagues at the National Research Council of well. Furthermore, the different types of lesion were Canada, the University of Manitoba, Central Medical shown to have distinct mid-IR signatures when Laboratories and the Misericordia General Hospital compared to adjacent normal epidermal and dermal in Winnipeg, Canada, we designed and patented a compartments. -
Targeting the Phosphatidylinositide-3 Kinase Pathway and the Mitogen
Targeting the Phosphatidylinositide-3 Kinase Pathway and the Mitogen-Activated-Protein Kinase Pathway through Thymosin-β4, Exercise, and Negative Regulators to Promote Retinal Ganglion Cell Survival or Regeneration by Mark Magharious A thesis submitted in conformity with the requirements for the degree of Master of Science Rehabilitation Sciences Institute University of Toronto © Copyright by Mark Magharious 2015 Targeting the Phosphatidylinositide-3 Kinase Pathway and the Mitogen-Activated-Protein Kinase Pathway through Thymosin-β4, Exercise, and Negative Regulators to Promote Retinal Ganglion Cell Survival or Regeneration Mark Magharious Master of Science Rehabilitation Sciences Institute University of Toronto 2015 Abstract The phosphatidylinositide-3 kinase (PI3K) and mitogen-activated-protein kinase (MAPK) pathways mediate cellular survival in the presence of apoptotic stimuli. These pathways are known to promote the survival of injured retinal ganglion cells (RGCs), central nervous system neurons that project visual information from the retina to the brain. Injury to the optic nerve triggers apoptosis of RGCs. This work demonstrates that Thymosin-β4, a peptide involved in actin sequestration, both enhances RGC survival after injury and increases axonal regeneration. Moreover, Thymosin-β4 modulates the PI3K and MAPK pathways. In addition, this study demonstrates that exercise reduces apoptosis of injured RGCs, and explores the function of the PI3K and MAPK pathways in this process. Finally, small peptides are used to interfere with the functions of PTEN, a negative regulator of the PI3K pathway, as well as Erbin and BCR, negative regulators in the MAPK pathway. These peptides enhance RGC survival and axonal regeneration after injury. ii Acknowledgments I would like to take this opportunity to recognize all those who helped me through the process of researching and writing this thesis. -
Sized Neuropeptides
M ETHODS IN MOLECULAR BIOLOGY™ Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Neuropeptides Methods and Protocols Edited by Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria, Università degli Studi di Torino, Grugliasco, TO, Italy; Istituto Nazionale di Neuroscienze (INN), Università degli Studi di Torino, Grugliasco, TO, Italy Editor Adalberto Merighi Dipartimento di Morfofisiologia Veterinaria Università degli Studi di Torino and Istituto Nazionale di Neuroscienze (INN) Università degli Studi di Torino Grugliasco, TO, Italy [email protected] Please note that additional material for this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-309-7 e-ISBN 978-1-61779-310-3 DOI 10.1007/978-1-61779-310-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011936011 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. -
Maccarrone-G.Pdf
Journal of Chromatography B, 1047 (2017) 131–140 Contents lists available at ScienceDirect Journal of Chromatography B jou rnal homepage: www.elsevier.com/locate/chromb MALDI imaging mass spectrometry analysis—A new approach for protein mapping in multiple sclerosis brain lesions a,b,1 a,1 c Giuseppina Maccarrone , Sandra Nischwitz , Sören-Oliver Deininger , a d,e d Joachim Hornung , Fatima Barbara König , Christine Stadelmann , b,1 a,f,∗,1 Christoph W. Turck , Frank Weber a Max Planck Institute of Psychiatry, Kraepelinstr. 2-10, 80804 Munich, Germany b Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Germany c Bruker Daltonik GmbH, Fahrenheitstr. 4, 28359 Bremen, Germany d Institute of Neuropathology, University Medical Center Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany e Institut für Pathologie, Klinikum Kassel, Mönchebergstr. 41-43, 34125 Kassel, Germany f Medical Park Bad Camberg, Obertorstr. 100-102, 65520 Bad Camberg, Germany a r t i c l e i n f o a b s t r a c t Article history: Multiple sclerosis is a disease of the central nervous system characterized by recurrent inflammatory Received 21 February 2016 demyelinating lesions in the early disease stage. Lesion formation and mechanisms leading to lesion Accepted 1 July 2016 remyelination are not fully understood. Matrix Assisted Laser Desorption Ionisation Mass Spectrom- Available online 1 July 2016 etry imaging (MALDI–IMS) is a technology which analyses proteins and peptides in tissue, preserves their spatial localization, and generates molecular maps within the tissue section. In a pilot study we Keywords: employed MALDI imaging mass spectrometry to profile and identify peptides and proteins expressed in MALDI imaging mass spectrometry normal-appearing white matter, grey matter and multiple sclerosis brain lesions with different extents LC–ESI–MS/MS of remyelination. -
RNA Sequencing Reveals the Alteration of the Expression of Novel Genes in Ethanol-Treated Embryoid Bodies
RESEARCH ARTICLE RNA Sequencing Reveals the Alteration of the Expression of Novel Genes in Ethanol-Treated Embryoid Bodies Chanchal Mandal1, Sun Hwa Kim1, Jin Choul Chai1, Seon Mi Oh1, Young Seek Lee1, Kyoung Hwa Jung2*, Young Gyu Chai1,3* 1 Department of Molecular and Life Science, Hanyang University, Ansan, Republic of Korea, 2 Institute of Natural Science and Technology, Hanyang University, Ansan, Republic of Korea, 3 Department of Bionanotechnology, Hanyang University, Seoul, Republic of Korea * [email protected] (YGC); [email protected] (KHJ) Abstract Fetal alcohol spectrum disorder is a collective term representing fetal abnormalities associ- OPEN ACCESS ated with maternal alcohol consumption. Prenatal alcohol exposure and related anomalies are well characterized, but the molecular mechanism behind this phenomenon is not well Citation: Mandal C, Kim SH, Chai JC, Oh SM, Lee characterized. In this present study, our aim is to profile important genes that regulate cellu- YS, Jung KH, et al. (2016) RNA Sequencing Reveals the Alteration of the Expression of Novel Genes in lar development during fetal development. Human embryonic carcinoma cells (NCCIT) are Ethanol-Treated Embryoid Bodies. PLoS ONE 11(3): cultured to form embryoid bodies and then treated in the presence and absence of ethanol e0149976. doi:10.1371/journal.pone.0149976 (50 mM). We employed RNA sequencing to profile differentially expressed genes in the eth- Editor: Shihui Yang, National Renewable Energy anol-treated embryoid bodies from NCCIT vs. EB, NCCIT vs. EB+EtOH and EB vs. EB Lab, UNITED STATES +EtOH data sets. A total of 632, 205 and 517 differentially expressed genes were identified Received: July 13, 2015 from NCCIT vs. -
The Human Gene Connectome As a Map of Short Cuts for Morbid Allele Discovery
The human gene connectome as a map of short cuts for morbid allele discovery Yuval Itana,1, Shen-Ying Zhanga,b, Guillaume Vogta,b, Avinash Abhyankara, Melina Hermana, Patrick Nitschkec, Dror Friedd, Lluis Quintana-Murcie, Laurent Abela,b, and Jean-Laurent Casanovaa,b,f aSt. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, NY 10065; bLaboratory of Human Genetics of Infectious Diseases, Necker Branch, Paris Descartes University, Institut National de la Santé et de la Recherche Médicale U980, Necker Medical School, 75015 Paris, France; cPlateforme Bioinformatique, Université Paris Descartes, 75116 Paris, France; dDepartment of Computer Science, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; eUnit of Human Evolutionary Genetics, Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, Institut Pasteur, F-75015 Paris, France; and fPediatric Immunology-Hematology Unit, Necker Hospital for Sick Children, 75015 Paris, France Edited* by Bruce Beutler, University of Texas Southwestern Medical Center, Dallas, TX, and approved February 15, 2013 (received for review October 19, 2012) High-throughput genomic data reveal thousands of gene variants to detect a single mutated gene, with the other polymorphic genes per patient, and it is often difficult to determine which of these being of less interest. This goes some way to explaining why, variants underlies disease in a given individual. However, at the despite the abundance of NGS data, the discovery of disease- population level, there may be some degree of phenotypic homo- causing alleles from such data remains somewhat limited. geneity, with alterations of specific physiological pathways under- We developed the human gene connectome (HGC) to over- come this problem. -
Dissecting Heterogeneous Cell Populations Across Drug and Disease Conditions with Popalign
Dissecting heterogeneous cell populations across drug and disease conditions with PopAlign Sisi Chena,b,1 , Paul Rivauda,b , Jong H. Parka,b, Tiffany Tsoua,b , Emeric Charlesc, John R. Haliburtond, Flavia Pichiorrie, and Matt Thomsona,b,1 aDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125; bBeckman Center for Single-Cell Profiling and Engineering, California Institute of Technology, Pasadena, CA 91125; cDepartment of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720; dAugmenta Bioworks Inc., Menlo Park, CA 94025; and eDepartment of Hematologic Malignancies Translational Science, City of Hope, Monrovia, CA 91016 Edited by Jonathan S. Weissman, University of California, San Francisco, CA, and approved September 25, 2020 (received for review March 31, 2020) Single-cell measurement techniques can now probe gene expres- The key conceptual advance underlying PopAlign is repre- sion in heterogeneous cell populations from the human body sentational: we model the distribution of gene-expression states across a range of environmental and physiological conditions. within a heterogeneous cell population using a probabilistic However, new mathematical and computational methods are mixture model that we infer from single-cell data. PopAlign required to represent and analyze gene-expression changes that identifies and represents subpopulations of cells as indepen- occur in complex mixtures of single cells as they respond to sig- dent Gaussian densities within a reduced -
Echinoderm Antimicrobial Peptides to Contrast Human Pathogens
s Chemis ct try u d & Schillaci and Arizza, Nat Prod Chem Res 2013, 1:2 o r R P e s l e a r a r u DOI: 10.4172/2329-6836.1000109 t c h a N Natural Products Chemistry & Research ISSN: 2329-6836 Review article Open Access Echinoderm Antimicrobial Peptides to Contrast Human Pathogens Domenico Schillaci1* and Vincenzo Arizza1,2 1Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi 18, - 90123 Palermo, Ital 2Istituto Euromediterraneo di Scienza e Biotecnologia - 90139 Palermo, Italy Abstract Increased attention has been focused in marine invertebrates as a source of bioactive molecules for biomedical applications. Many bioactive molecules are part of the innate immune system. Some more recently isolated compounds, mainly from sea urchin and sea cucumber are antimicrobial peptides (AMP) active against Gram positive, Gram negative and fungi. In this review we described the most recent studies on AMP isolated from echinoderms. The AMP are little peptides <10 kDa with cationic charge and amphipathic structure. Recently, it was demonstrated that in the coelomocyte lysates of Paracentrotus. lividus and Holothuria tubulosa AMP are present with activity against staphylococcal and Pseudomonas aeruginosa antibiofilm. The data show the great potential of application of AMPs in biotechnology for developing novel therapeutic agents and as complements to conventional antibiotic therapy to combat the multi - resistant bacterial strains. Keywords: Antimicrobial peptides; Therapeutic agents; Antibiotic nucleic acids and/or enzymatic proteins, leading to bacterial cell death therapy [10-14]. Moreover, it appears that many AMPs may be multifunctional microbicides, acting simultaneously at the cell membrane and internal Introduction sites [11]. -
Robust Hypergraph Regularized Non-Negative Matrix Factorization for Sample Clustering and Feature Selection in Multi-View Gene E
Yu et al. Human Genomics 2019, 13(Suppl 1):46 https://doi.org/10.1186/s40246-019-0222-6 RESEARCH Open Access Robust hypergraph regularized non- negative matrix factorization for sample clustering and feature selection in multi- view gene expression data Na Yu1, Ying-Lian Gao2, Jin-Xing Liu1*, Juan Wang1* and Junliang Shang1 From IEEE International Conference on Bioinformatics and Biomedicine 2018 Madrid, Spain. 3-6 December 2018 Abstract Background: As one of the most popular data representation methods, non-negative matrix decomposition (NMF) has been widely concerned in the tasks of clustering and feature selection. However, most of the previously proposed NMF-based methods do not adequately explore the hidden geometrical structure in the data. At the same time, noise and outliers are inevitably present in the data. Results: To alleviate these problems, we present a novel NMF framework named robust hypergraph regularized non-negative matrix factorization (RHNMF). In particular, the hypergraph Laplacian regularization is imposed to capture the geometric information of original data. Unlike graph Laplacian regularization which captures the relationship between pairwise sample points, it captures the high-order relationship among more sample points. Moreover, the robustness of the RHNMF is enhanced by using the L2,1-norm constraint when estimating the residual. This is because the L2,1-norm is insensitive to noise and outliers. Conclusions: Clustering and common abnormal expression gene (com-abnormal expression gene) selection are conducted to test the validity of the RHNMF model. Extensive experimental results on multi-view datasets reveal that our proposed model outperforms other state-of-the-art methods. -
Views of the NIDA, NINDS Or the National Summed Across the Three Auditory Forebrain Lobule Sec- Institutes of Health
Xie et al. BMC Biology 2010, 8:28 http://www.biomedcentral.com/1741-7007/8/28 RESEARCH ARTICLE Open Access The zebra finch neuropeptidome: prediction, detection and expression Fang Xie1, Sarah E London2,6, Bruce R Southey1,3, Suresh P Annangudi1,6, Andinet Amare1, Sandra L Rodriguez-Zas2,3,5, David F Clayton2,4,5,6, Jonathan V Sweedler1,2,5,6* Abstract Background: Among songbirds, the zebra finch (Taeniopygia guttata) is an excellent model system for investigating the neural mechanisms underlying complex behaviours such as vocal communication, learning and social interactions. Neuropeptides and peptide hormones are cell-to-cell signalling molecules known to mediate similar behaviours in other animals. However, in the zebra finch, this information is limited. With the newly-released zebra finch genome as a foundation, we combined bioinformatics, mass-spectrometry (MS)-enabled peptidomics and molecular techniques to identify the complete suite of neuropeptide prohormones and final peptide products and their distributions. Results: Complementary bioinformatic resources were integrated to survey the zebra finch genome, identifying 70 putative prohormones. Ninety peptides derived from 24 predicted prohormones were characterized using several MS platforms; tandem MS confirmed a majority of the sequences. Most of the peptides described here were not known in the zebra finch or other avian species, although homologous prohormones exist in the chicken genome. Among the zebra finch peptides discovered were several unique vasoactive intestinal and adenylate cyclase activating polypeptide 1 peptides created by cleavage at sites previously unreported in mammalian prohormones. MS-based profiling of brain areas required for singing detected 13 peptides within one brain nucleus, HVC; in situ hybridization detected 13 of the 15 prohormone genes examined within at least one major song control nucleus. -
FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen 2003
Boeken zonder publiciteit FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen NATIONAAL FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Interuniversitair Instituut voor Kernwetenschappen Fonds voor Geneeskundig Wetenschappelijk Onderzoek 2003 Lijst der kredietgenieters (Met opgave van hun onderzoeksprogramma en onthaalinstelling) Egmontstraat 5 1000 BRUSSEL Tel. (02) 512.91.10 With English Summary and Abstracts FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Vlaanderen NATIONAAL FONDS VOOR WETENSCHAPPELIJK ONDERZOEK Interuniversitair Instituut voor Kernwetenschappen Fonds voor Geneeskundig Wetenschappelijk Onderzoek 2003 Lijst der kredietgenieters (Met opgave van hun onderzoeksprogramma en onthaalinstelling) Egmontstraat 5 1000 BRUSSEL Tel. (02) 512.91.10 AFKORTINGEN A.R.A. : Algemeen Rijksarchief en K.M.I. : Koninklijk Meteorologisch Rijksarchief in de Provinciën Instituut van België B.I.R.A. : Belgisch Instituut voor K.M.K.G. : Koninklijke Musea voor Ruimte-Aëronomie Kunst en Geschiedenis B.R. : Bibliothèque Royale Albert K.M.M.A. : Koninklijk Museum voor Ier Midden-Afrika C.E.G.E.S. : Centre d’Etudes et de Docu- K.M.S. : Koninklijke Militaire mentation Guerre et Sociétés School contemporaines K.M.S.K.B. : Koninklijke Musea voor C.L.O.Gent: Centrum voor Landbouwkun- Schone Kunsten van België dig Onderzoek - Gent K.S.B. : Koninklijke Sterrenwacht C.O.D.A. : Centrum voor Onderzoek in van België Diergeneeskunde en Agro- K.U.Brussel: Katholieke Universiteit chemie Brussel E.R.M. : Ecole Royale Militaire K.U.Leuven : Katholieke Universiteit E.S.K.E. : Egyptologische Stichting Leuven Koningin Elisabeth L.U.C. : Limburgs Universitair Cen- F.N.R.S. Fonds National de la Recher- trum che Scientifique M.R.A.C. : Musée Royal de l'Afrique F.P.Ms : Faculté Polytechnique de Centrale Mons N.F.W.O.