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WO 2012/112524 A2 23 August 2012 (23.08.2012) P O P C T

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WO 2012/112524 A2 23 August 2012 (23.08.2012) P O P C T (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2012/112524 A2 23 August 2012 (23.08.2012) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 5/(94 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, (21) International Application Number: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, PCT/US20 12/0250 18 DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (22) International Filing Date: HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, 14 February 2012 (14.02.2012) KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, (26) Publication Language: English SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 61/442,744 14 February 201 1 (14.02.201 1) US (84) Designated States (unless otherwise indicated, for every PCT/US201 1/024936 kind of regional protection available): ARIPO (BW, GH, 15 February 201 1 (15.02.201 1) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, 13/258,653 22 September 201 1 (22.09.201 1) US UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, 13/303,433 23 November 201 1 (23. 11.201 1) US TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (71) Applicant (for all designated States except US): THE LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, RED TRIANGLE, LLC [US/US]; 17618 Dunbar Road, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Mount Vernon, Washington 98273 (US). GW, ML, MR, NE, SN, TD, TG). (72) Inventors; and Declarations under Rule 4.17 : (75) Inventors/Applicants (for US only): BURR, Randall, W. — as to applicant's entitlement to applyfor and be granted a [US/US]; 19698 Montpiliar Place, Mount Vernon, WA patent (Rule 4.1 7(H)) 98274 (US). HEINRICHER, Jackie R. [US/US]; 5722 Campbell Lake Rd., Anacortes, Washington 98221 (US). Published: (74) Agent: VEINTENHEIMER, Eric, E.; 777 6th Street, — without international search report and to be republished N.W., Suite 1100, Washington, District of Columbia upon receipt of that report (Rule 48.2(g)) 20001 (US). < o (54) Title: MEDIA, KITS, SYSTEMS AND METHODS FOR THE MICROPROPAGATION OF MONOCOTYLEDONOUS PLANTS (57) Abstract: Disclosed herein are media, systems and methods for achieving micropropagation of monocotyledonous plants. MEDIA, KITS, SYSTEMS AND METHODS FOR THE MICROPROPAGATION OF MONOCOTYLEDONOUS PLANTS CROSS-REFERENCE TO RELATED APPLICATIONS [0001 ] This application claims priority to and the benefit of U.S. Patent Application No. 13/303,433, filed November 23, 201 1, which is a continuation- in-part of U.S. Patent Application No. 13/258,653, filed September 22, 201 1, which is a National Stage Entry of International Patent Application No. PCT/US201 1/024936, filed February 15, 201 1. This application also claims priority to and the benefit of U.S. Provisional Patent Application No. 61/442,744, filed February 14, 201 1. The entire disclosures of U.S. Patent Application Nos. 13/303,433 and 13/258,653, International Patent Application No. PCT/US201 1/024936, and U.S. Provisional Patent Application No. 61/442,744 are incorporated by reference herein in their entireties. FIELD OF THE DISCLOSURE [0002] Disclosed herein are media, kits, systems and methods for achieving micropropagation of plants, particularly monocotyledonous plants ("monocots"). BACKGROUND OF THE DISCLOSURE [0003] Micropropagation (also known as tissue culturing with the terms used interchangeably herein) is an excellent method to achieve large scale production of many plants. Micropropagation is not unlike growing plants from cuttings. However, unlike plants grown from cuttings, micropropagated plants are grown in vitro in sterile media. Typically, the media comprises agar, with the addition of various compounds such as nutrients, inorganic salts, growth regulators, sugars, vitamins and other compounds. [0004] A benefit to tissue culturing plants is that the plants can be grown in a sterile environment so that they remain disease free. Other benefits include the ability to grow very large numbers of plants in a small space, the reduced water and nutrient needs of micropropagated plants, and the rapid multiplication of tissues that can in turn be used to yield more tissue culture material. Moreover micropropagation is very flexible and rapid upscaling is possible (within 1 year nearly one million plants can be produced from any genotype). Such short time frames and large numbers cannot be rivaled by any conventional method. Tissue culturing also provides for the production of high quality plants which are easy to transport and deliver. [0005] A number of plants have never been successfully micropropagated on a large scale. For example, while some papers have been published which address tissue culturing of monocot, in practice, the described methods have not translated into commercially viable propagation systems. SUMMARY OF THE DISCLOSURE [0006] The present disclosure provides effective media, kits, systems and methods for tissue culturing plants on a commercial scale. [0007] One embodiment disclosed herein is a media for micropropagating plants wherein said media comprises meta-topolin and/or thidiazuron. In another embodiment, the media comprises meta-topolin. [0008] In another embodiment, the media is b-9-i media, b-9-ii media, b-9-iii media, b-9-iv media, b-9-v media, CW2-i media, CW 2- media, CW2-iii media, CW2-iv media, CW2-v media, b-1 0-i media, b-1 0-ii media, b-1 0-iii media, b- 10-iv media, b-1 0-v media, b-1 1-i media, b-1 1-ii media, b-1 1-iii media, b-1 1-iv media, b-1 1-v media, b-1 2c-i media, b-12c-ii media, b-1 2c-iii media, b-1 2c-iv media, b-1 2c-v media, b-1 -i media, b-1-ii media, b-1-iii media, b-1 -iv media, b- 1-v media, b-4-i media, b-4-ii media, b-4-iii media, b-4-iv media, b-4-v media, b-6-i media, b-6-ii media, b-6-iii media, b-6-iv media, b-6-v media, CW1 -i media, CW1 -ii media, CW1 -iii media, CW1 -iv media, CW1 -v media, CW3-i media, CW3-ii media, CW3-iii media, CW3-iv media, CW3-v media, CW4-i media, CW4-ii media, CW4-iii media, CW4-iv media, CW4-v media, CW5-i media, CW5-ii media, CW5-iii media, CW5-iv media, CW5-v media, CW6-i media, CW 6- media, CW6-iii media, CW6-iv media, CW6-v media, R 1-i media, R 1- media, R 1-iii media, R 1-iv media, R 1-v media, R2-i media, R2- media, R2-iii media, R2-iv media, R2-v media, R3-i media, R3- media, R3-iii media, R3-iv media, R3-v media, R4-i media, R4 - media, R4-iii media, R4-iv media or R4-v media. [0009] One embodiment disclosed herein is a kit for micropropagating plants wherein said kit includes a media comprising meta-topolin and/or thidiazuron. In another embodiment, the media comprises meta-topolin. In another embodiment, the media is b-9-i media, b-9-ii media, b-9-iii media, b-9-iv media, b-9-v media, CW2-i media, CW 2- media, CW2-iii media, CW2-iv media, CW2-v media, b-10-i media, b-1 0-ii media, b-1 0-iii media, b-1 0-iv media, b-1 0-v media, b-1 1-i media, b-1 1-ii media, b-1 1-iii media, b-1 1-iv media, b-1 1-v media, b-1 2c-i media, b-12c-ii media, b-1 2c-iii media, b-1 2c-iv media, b-1 2c-v media, b-1 -i media, b-1-ii media, b-1-iii media, b-1 -iv media, b- 1-v media, b-4-i media, b-4-ii media, b-4-iii media, b-4-iv media, b-4-v media, b-6-i media, b-6-ii media, b-6-iii media, b-6-iv media, b-6-v media, CW1 -i media, CW1 -ii media, CW1 -iii media, CW1 -iv media, CW1 -v media, CW3-i media, CW3-ii media, CW3-iii media, CW3-iv media, CW3-v media, CW4-i media, CW4-ii media, CW4-iii media, CW4-iv media, CW4-v media, CW5-i media, CW5-ii media, CW5-iii media, CW5-iv media, CW5-v media, CW6-i media, CW6-ii media, CW6-iii media, CW6-iv media, CW6-v media, R 1-i media, R 1-ii media, R 1-iii media, R 1-iv media, R 1-v media, R2-i media, R2-ii media, R2-iii media, R2-iv media, R2-v media, R3-i media, R3-ii media, R3-iii media, R3-iv media, R3-v media, R4-i media, R4-ii media, R4-iii media, R4-iv media or R4-v media.
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