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(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2012/112524 A2 23 August 2012 (23.08.2012) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 5/(94 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, (21) International Application Number: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, PCT/US20 12/0250 18 DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (22) International Filing Date: HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, 14 February 2012 (14.02.2012) KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, (26) Publication Language: English SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 61/442,744 14 February 201 1 (14.02.201 1) US (84) Designated States (unless otherwise indicated, for every PCT/US201 1/024936 kind of regional protection available): ARIPO (BW, GH, 15 February 201 1 (15.02.201 1) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, 13/258,653 22 September 201 1 (22.09.201 1) US UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, 13/303,433 23 November 201 1 (23. 11.201 1) US TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (71) Applicant (for all designated States except US): THE LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, RED TRIANGLE, LLC [US/US]; 17618 Dunbar Road, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Mount Vernon, Washington 98273 (US). GW, ML, MR, NE, SN, TD, TG). (72) Inventors; and Declarations under Rule 4.17 : (75) Inventors/Applicants (for US only): BURR, Randall, W. — as to applicant's entitlement to applyfor and be granted a [US/US]; 19698 Montpiliar Place, Mount Vernon, WA patent (Rule 4.1 7(H)) 98274 (US). HEINRICHER, Jackie R. [US/US]; 5722 Campbell Lake Rd., Anacortes, Washington 98221 (US). Published: (74) Agent: VEINTENHEIMER, Eric, E.; 777 6th Street, — without international search report and to be republished N.W., Suite 1100, Washington, District of Columbia upon receipt of that report (Rule 48.2(g)) 20001 (US). < o (54) Title: MEDIA, KITS, SYSTEMS AND METHODS FOR THE MICROPROPAGATION OF MONOCOTYLEDONOUS PLANTS (57) Abstract: Disclosed herein are media, systems and methods for achieving micropropagation of monocotyledonous plants. MEDIA, KITS, SYSTEMS AND METHODS FOR THE MICROPROPAGATION OF MONOCOTYLEDONOUS PLANTS CROSS-REFERENCE TO RELATED APPLICATIONS [0001 ] This application claims priority to and the benefit of U.S. Patent Application No. 13/303,433, filed November 23, 201 1, which is a continuation- in-part of U.S. Patent Application No. 13/258,653, filed September 22, 201 1, which is a National Stage Entry of International Patent Application No. PCT/US201 1/024936, filed February 15, 201 1. This application also claims priority to and the benefit of U.S. Provisional Patent Application No. 61/442,744, filed February 14, 201 1. The entire disclosures of U.S. Patent Application Nos. 13/303,433 and 13/258,653, International Patent Application No. PCT/US201 1/024936, and U.S. Provisional Patent Application No. 61/442,744 are incorporated by reference herein in their entireties. FIELD OF THE DISCLOSURE [0002] Disclosed herein are media, kits, systems and methods for achieving micropropagation of plants, particularly monocotyledonous plants ("monocots"). BACKGROUND OF THE DISCLOSURE [0003] Micropropagation (also known as tissue culturing with the terms used interchangeably herein) is an excellent method to achieve large scale production of many plants. Micropropagation is not unlike growing plants from cuttings. However, unlike plants grown from cuttings, micropropagated plants are grown in vitro in sterile media. Typically, the media comprises agar, with the addition of various compounds such as nutrients, inorganic salts, growth regulators, sugars, vitamins and other compounds. [0004] A benefit to tissue culturing plants is that the plants can be grown in a sterile environment so that they remain disease free. Other benefits include the ability to grow very large numbers of plants in a small space, the reduced water and nutrient needs of micropropagated plants, and the rapid multiplication of tissues that can in turn be used to yield more tissue culture material. Moreover micropropagation is very flexible and rapid upscaling is possible (within 1 year nearly one million plants can be produced from any genotype). Such short time frames and large numbers cannot be rivaled by any conventional method. Tissue culturing also provides for the production of high quality plants which are easy to transport and deliver. [0005] A number of plants have never been successfully micropropagated on a large scale. For example, while some papers have been published which address tissue culturing of monocot, in practice, the described methods have not translated into commercially viable propagation systems. SUMMARY OF THE DISCLOSURE [0006] The present disclosure provides effective media, kits, systems and methods for tissue culturing plants on a commercial scale. [0007] One embodiment disclosed herein is a media for micropropagating plants wherein said media comprises meta-topolin and/or thidiazuron. In another embodiment, the media comprises meta-topolin. [0008] In another embodiment, the media is b-9-i media, b-9-ii media, b-9-iii media, b-9-iv media, b-9-v media, CW2-i media, CW 2- media, CW2-iii media, CW2-iv media, CW2-v media, b-1 0-i media, b-1 0-ii media, b-1 0-iii media, b- 10-iv media, b-1 0-v media, b-1 1-i media, b-1 1-ii media, b-1 1-iii media, b-1 1-iv media, b-1 1-v media, b-1 2c-i media, b-12c-ii media, b-1 2c-iii media, b-1 2c-iv media, b-1 2c-v media, b-1 -i media, b-1-ii media, b-1-iii media, b-1 -iv media, b- 1-v media, b-4-i media, b-4-ii media, b-4-iii media, b-4-iv media, b-4-v media, b-6-i media, b-6-ii media, b-6-iii media, b-6-iv media, b-6-v media, CW1 -i media, CW1 -ii media, CW1 -iii media, CW1 -iv media, CW1 -v media, CW3-i media, CW3-ii media, CW3-iii media, CW3-iv media, CW3-v media, CW4-i media, CW4-ii media, CW4-iii media, CW4-iv media, CW4-v media, CW5-i media, CW5-ii media, CW5-iii media, CW5-iv media, CW5-v media, CW6-i media, CW 6- media, CW6-iii media, CW6-iv media, CW6-v media, R 1-i media, R 1- media, R 1-iii media, R 1-iv media, R 1-v media, R2-i media, R2- media, R2-iii media, R2-iv media, R2-v media, R3-i media, R3- media, R3-iii media, R3-iv media, R3-v media, R4-i media, R4 - media, R4-iii media, R4-iv media or R4-v media. [0009] One embodiment disclosed herein is a kit for micropropagating plants wherein said kit includes a media comprising meta-topolin and/or thidiazuron. In another embodiment, the media comprises meta-topolin. In another embodiment, the media is b-9-i media, b-9-ii media, b-9-iii media, b-9-iv media, b-9-v media, CW2-i media, CW 2- media, CW2-iii media, CW2-iv media, CW2-v media, b-10-i media, b-1 0-ii media, b-1 0-iii media, b-1 0-iv media, b-1 0-v media, b-1 1-i media, b-1 1-ii media, b-1 1-iii media, b-1 1-iv media, b-1 1-v media, b-1 2c-i media, b-12c-ii media, b-1 2c-iii media, b-1 2c-iv media, b-1 2c-v media, b-1 -i media, b-1-ii media, b-1-iii media, b-1 -iv media, b- 1-v media, b-4-i media, b-4-ii media, b-4-iii media, b-4-iv media, b-4-v media, b-6-i media, b-6-ii media, b-6-iii media, b-6-iv media, b-6-v media, CW1 -i media, CW1 -ii media, CW1 -iii media, CW1 -iv media, CW1 -v media, CW3-i media, CW3-ii media, CW3-iii media, CW3-iv media, CW3-v media, CW4-i media, CW4-ii media, CW4-iii media, CW4-iv media, CW4-v media, CW5-i media, CW5-ii media, CW5-iii media, CW5-iv media, CW5-v media, CW6-i media, CW6-ii media, CW6-iii media, CW6-iv media, CW6-v media, R 1-i media, R 1-ii media, R 1-iii media, R 1-iv media, R 1-v media, R2-i media, R2-ii media, R2-iii media, R2-iv media, R2-v media, R3-i media, R3-ii media, R3-iii media, R3-iv media, R3-v media, R4-i media, R4-ii media, R4-iii media, R4-iv media or R4-v media.
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  • THE BAMBOOS of NEPAL and BHUTAN PART III: Drepanostachyum, Himalayacalamus, Ampelocalamus, Neomicrocalamus and Chimonobambusa (Gramineae: Poaceae, Bambusoideae)

    THE BAMBOOS of NEPAL and BHUTAN PART III: Drepanostachyum, Himalayacalamus, Ampelocalamus, Neomicrocalamus and Chimonobambusa (Gramineae: Poaceae, Bambusoideae)

    EDINB. J. BOT. 51(3): 301–330 (1994) THE BAMBOOS OF NEPAL AND BHUTAN PART III: Drepanostachyum, Himalayacalamus, Ampelocalamus, Neomicrocalamus and Chimonobambusa (Gramineae: Poaceae, Bambusoideae) C. M. A. S TAPLETON * This paper completes the systematic treatment of the bamboos of Nepal and Bhutan, covering five genera from subtropical to lower temperate zones. Three further genera from the subtribe Arundinariinae Bentham are included: Drepanostachyum Keng f., Himalayacalamus Keng f., and Ampelocalamus Chen, Wen & Sheng . They have semelauctant ebracteate inflorescences, pachymorph rhizomes, and 3 stamens. Neomicrocalamus Keng f. has semel- auctant bracteate inflorescences and 6 stamens, and is in the new subtribe described here, Racemobambosinae. Chimonobambusa Makino has bracteate inflorescences and 3 stamens and is the only Himalayan genus in the subtribe Shibataeinae (Nakai) Soderstrom & Ellis. A new Drepanostachyum species from Bhutan is described as D. annulatum. Himalayacalamus , which was originally described as a monotypic genus, is enlarged by the description of five new species, H. asper , H. brevinodus , H. cupreus , H. fimbriatus , and H. porcatus , all from Nepal. A Himalayan representative of the genus Ampelocalamus , A. patellaris , is transferred from Dendrocalamus. Neomicrocalamus andro- pogonifolius from eastern Bhutan is transferred from Bambusa . STATUS AND S EPARATION OF THE G ENERA These genera have all been considered to be part of Arundinaria Michaux at one time. The type species of the genera Drepanostachyum Keng f. , Ampelocalamus Chen, Wen & Sheng, Neomicrocalamus Keng f., and Chimonobambusa Makino were originally described as species of Arundinaria Michaux, while the type species of Himalayacalamus was initially described as a species of Thamnocalamus Munro, before being transferred into Arundinaria .
  • Dear Author, Please, Note That Changes Made to the HTML Content

    Dear Author, Please, Note That Changes Made to the HTML Content

    Dear Author, Please, note that changes made to the HTML content will be added to the article before publication, but are not reflected in this PDF. Note also that this file should not be used for submitting corrections. YMPEV 5375 No. of Pages 12, Model 5G 26 December 2015 Molecular Phylogenetics and Evolution xxx (2015) xxx–xxx 1 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev 5 6 3 Multi-locus plastid phylogenetic biogeography supports the Asian q 4 hypothesis of the temperate woody bamboos (Poaceae: Bambusoideae) a,b,d b a,b c a,b 7 Xian-Zhi Zhang , Chun-Xia Zeng , Peng-Fei Ma , Thomas Haevermans , Yu-Xiao Zhang , a,b,d b,⇑ a,b,⇑ 8 Li-Na Zhang , Zhen-Hua Guo , De-Zhu Li 9 a Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China 10 b Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming, Yunnan 650201, China 11 c Institut de Systématique, Évolution, Biodiversité ISYEB, Unité Mixte de Recherche 7205, Centre National de la Recherche Scientifique/Muséum national d’histoire naturelle/École 12 Pratique des Hautes Études/Université Pierre-et-Marie-Curie, Muséum national d’histoire naturelle, Sorbonne Universités, 57 Rue Cuvier, CP39, F-75231 Paris cedex 05, France 13 d Kunming College of Life Sciences, University of Chinese Academy of Sciences, Kunming, Yunnan 650201, China 1415 16 article info abstract 3218 19 Article history: In this paper we investigate the biogeography of the temperate woody bamboos (Arundinarieae) using a 33 20 Received 19 June 2015 densely-sampled phylogenetic tree of Bambusoideae based on six plastid DNA loci, which corroborates 34 21 Revised 22 November 2015 the previously discovered 12 lineages (I–XII) and places Kuruna as sister to the Chimonocalamus clade.