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National Institute of Genetics Japan ISSN 0077-4995 NATIONAL INSTITUTE OF GENETICS JAPAN ANNUAL REPORT No. 39 1988 Published by THE NATIONAL INSTITUTE OF GENETICS Misima, Sizuoka-ken, Japan 1989 Annual Report of the National Institute of Genetics No. 39, 1988 Published by The National Institute of Genetics, Japan 1989 CONTENTS General statement .............................................. 1 Staff 7 Council and Advisory Committee '" .. .. 10 Association for propagation of the knowledge of genetics 12 Projects of research for 1988 13 Research activities in 1988 18 1. Molecular Genetics Promoter selectivity of E. coli RNA polymerase: Cloning and characterization ofE. coli promoters. KUBOTA, M., YAMAZAKI, Y., FUJITA, N. and ISHIHAMA, A. 18 Promoter selectivity of E. coli RNA polymerase: Subunit (j) plays an essential role in stringent control. IGARASHI, K., FUJITA, N., OKAMOTO, H. and ISHIHAMA, A. 19 RNA polymerase sigma-related protein in Escherichia coli: Puri­ fication and characterization of proteins which crossreact with anti-sigma peptide antibodies. UESHIMA, R., FUJITA, N. and ISHIHAMA, A. 20 RNA polymerase and transcription signals of Micrococcus luteus. NAKAYAMA, M., FUJITA, N., OSAWA, S. and ISHIHAMA, A. '" 20 Molecular mechanism of influenza virus RNA transcription. I. Analysis of promoters for viral RNA polymerase. ISHIHAMA, A., PARVIN, J. D., PALESE, P., KRYSTAL, M., HONDA, A., KATO, A., UEDA, S., YOKOIYAMA, A., YAMAZAKI, Y., YAMA- NAKA, K. and NAGATA, K. 21 Molecular mechanism of influenza virus RNA transcription. II. Matrix protein epitopes involved in transcription inhibition. HANKINS, R. W., NAGATA, K., BUCHER, D. J., and ISHIHAMA, A 22 Molecular mechanism of influenza virus RNA transcription. III. Binding mode of influenza virus nucleoprotein to RNA. YAMANAKA, K., OGASAWARA, N., YOSHIKAWA, H., NAGATA, K. and ISHIHAMA, A. 23 ii ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 39 Transcription and replication of influenza virus RNA: An in vitro system using isolation nuclei from virus-infected cells. NAGA- TA, K., SAKAGAMI, H. and ISHIHAMA, A. 24 Translational regulation of influenza virus mRNAs. YAMANAKA, K., OGASAWARA, N., YOSHIKAWA, H., NAGATA, K. and ISHI- HAMA. A 25 Mechanism of anti-influenza virus by mouse Mx gene. YOKOI­ YAMA, A., NAGATA, K., IWAKURA, Y., and ISHIHAMA, A. .... 25 Regulation of transcription by nuclear factor I, a protein essential for adenovirus DNA replication. MATSUMOTO, K., NAGATA, K., HANAOKA, F. and ISHIHAMA, A. 26 Soluble 5'-nucleotidases from rabbit heart. YAMAZAKI, Y., TRUONG, Y. L., LOWENSTEIN, J. M., and ISHIHAMA, A. 27 Structure and complete nucleotide sequence of the h~man thy­ midylate synthase gene. KANEDA, S., NALBANTOGLU, J., TAKEISHI, K., AYUSAWA, D., SHIMIZU, K., GOTOH, 0., HORIE, N. and SENO, T. ...................................... .. 28 Functional analysis of the human thymidylate synthase gene with the use of a minigene. KANEDA, S., AYUSAWA, D. and SENO, T. 29 Characterization of DNA double strand scission in mouse cells induced by thymidylate deprivation. AYUSAWA, D., TEZUKA, H. and SENO, T. 30 Coupling between replication and translation by E. coli single- stranded DNA binding protein. SHIMAMOTO, N. 31 Kinetic study on transcription by immobilized operons: Release of E. coli RNA polymerase (J subunit requires f3-r phosphodiester bond of nucleoside triphosphates. SHIMAMOTO, N. and FUJIOKA, M. 31 Global variation in G+C content along vertebrate genome DNA, and chromosome band structures. IKEMURA, T., AOTA, S., ISHIBASHI, F. and MATSUMOTO, K. 32 Purification of RNA molecules with high resolution by two- dimensional gel techniques. IKEMURA, T. 33 Computer analyses for searching for protein coding regions in human genome DNA sequences. WADA, K., AOTA, S. and IKEMURA, T. 34 CONTENTS iii Purification of a DNA supercoiling factor from the posterior silk gland of Bombyx mori. OHTA, T. and HIROSE, S. 34 DNA supercoiling facilitates formation of the transcription initia- tion complex on the fibroin gene promoter. TABUCHI, H. and HIROSE, S. ....................................... .. 35 Mechanism of mRNA capping reaction. MIZUMOTO, K. 36 Messenger RNA synthesis of Sendai virus (HVJ). MIZUMOTO, K 37 Regulation of chromosomal replication in bacteria. YOSHIKAWA, H 38 II. Microbial Genetics Gene organization in the region containing a new gene involved in chromosome partition in Escherichia coli. KATO, J., NISHIMURA, Y., YAMADA, M., SUZUKI, H. and HIROTA, Y. .. 41 Isolation of novel mutants defective in DNA replication in Es- cherichia coli. YASUDA, S. 43 AIlelism of Escherichia coli parA to gyrB and some views on par­ dependent regulation of ceIl division. NISHIMURA, Y., KATO, J. and SUZUKI, H. .. .................................. .. 45 Nucleotide sequence of the sufI gene responsible for the multicopy suppression of a thermosensitive cell-division mutation in E. coli. YAMAMOTO, Y., KATO, J., SUZUKI, H. and NISHI- MURA, Y 47 C-terminal processing of penicillin-binding protein 3 of Escherichia coli. I. Analysis ofthe degradation product that represents the C-terminal half. SAKAGAMI, Y., NAGASAWA, H., SUZUKI, A., HARA, H. and SUZUKI, H. ............................... 48 C-terminal processing of penicillin-binding protein 3 of Escherichia coli. II. Determination of the cleavage site. NAGASAWA, H., SUZUKI, A., HARA, H. and SUZUKI, H. .. ................ .. 49 Requirement of the C-terminal region for the function of Escheri- chia coli penicillin-binding protein 3. HARA, H. and SUZUKI, H. 51 Cloning ofa gene involved in the C-terminal processing ofpenicillin­ binding protein 3 of Escherichia coli. HARA, H. and SUZUKI, H 52 iv ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 39 Mapping of prc that causes a defect in the C-terminal processing in Escherichia coli. HARA, H., HIGASHITANI, A., NISHIMURA, Y. and SUZUKI, H. 55 Accumulation of the precursor form of penicillin-binding protein 3 in Escherichia coli mutants defective in general protein ex- port. HARA, H. and SUZUKI, H. 56 A new gene controlling the frequency of cell division per round of DNA replication in Escherichia coli. NISHIMURA, A. 57 A cell division regulatory mechanism controls the flagellar regulon in Escherichia coli. NISHIMURA, A. and HIROTA, Y. 58 Restriction map of a Bacillus subtilis gene involved in cell division, sporulation and exoenzyme secretion. SADAIE, Y. 59 III. Immunogenetics Novel molecular organization of meiotic recombinational hotspots in the murine major histocompatibility complex. SHIROISHI, T., SAGAI, T., HANZAWA, N., STEINMETZ, M. and MORIWAKI, K 60 New evidence for trans-species evolution of the H-2 class I poly­ morphism. SAGAI, T., SHIROISHI, T. and MORIWAKI, K. .... 61 Steroid 21-hydroxylase deficiency in mice. GOTOH, H., SAGAI, T., RATA, J., SHIROISHI, T. and MORIWAKI, K. 62 IV. Developmental and Somatic Cell Genetics Artificial expression of tissue-specific characteristics in embryonic lethal cells of Drosophila melanogaster. KURODA, Y. 63 Selective infection of SR spiroplasms in cultured embryonic cells of Drosophila melanogaster. KURODA, Y., SAKAGUCHI, B., OISHI, K. and SHIMADA, Y. 64 Antimutagenic effects of safrole on chemically induced mutations in cultured Chinese hamster cells. KURODA, Y., JAIN, A. K. and TEZUKA, H. 65 Injury effect on hydra head regeneration. SHIMIZU, H. and SUGI- YAMA, T 66 Random displacement of I-cells into buds and maintenance of the I-cell population in Hydra. FUJISAWA, T. 67 CONTENTS v Genetic studies on the silkworm adult lifespan (a). Heredity of the short adult lifespan (sdi). MURAKAMI, A. 67 Genetic studies on the silkworm adult lifespan (b). A biological characteristic of the Daizo (sdi) strain. MURAKAMI, A. 69 Genetic studies on the silkworm adult lifespan (c). Sex differences and their biological significance. MURAKAMI, A. 70 The genetic nature of known mutants in the domesticated mulberry silkworm, Bombyx mori. MURAKAMI, A. 71 Decrease in hatchability of eggs laid by flies reared on excess yeast in Drosophila melanogaster. MINATO, K. 73 Purification and structure analysis of an autocrine growth factor in conditioned medium obtained from primary cultures of scleral fibroblasts of the chick embryo. FUJIOKA, M., KAWA­ HARA, A., AMANO, M., WATANABE, K. and SHIMAMOTO, N ... 74 The three-dimensional ultrastructure of intracellular membranous system in cultured chick skeletal muscle cells. SHIMADA, Y. 75 v. Cytogenetics Molecular analysis of complement factor H genes in wild mice (Mus musculus) and their relatives. HARADA, Y., BONHOMME, F., NATSUUME-SAKAI, S., MORIWAKI, K. and TOMITA, T..... 76 Reproductive ability of hybrids between Mus musculus castaneus and M. m. subspecies in China. NIWA, M., WAKASUGI, N., MITA, A. and MORIWAKI, K . 77 Effect of the Y chromosome and H-2 complex of Japanese wild mouse on sperm morphology. SUH, D.-S., STYRNA, J. and MORIWAKI, K . 78 DNA sequencing analysis of rDNA in Robertsonian mice. KURI­ HARA, Y., SUZUKI, H., WINKING, H., BONHOMME, F. and MORI- WAKI, K . 79 Geographical survey of wild mouse populations in Eastern Asia based on restriction analysis and sequence analysis of mito­ chondrial DNA. YONEKAWA, H., MIYASHITA, N. and MORI- WAKI, K . 79 vi ANNUAL REPORT OF NATIONAL INSTITUTE OF GENETICS NO. 39 VI. Mutagenesis and Radiation Genetics Tumor suppression gene in Asian wild-derived strains of mice. MIYASHITA, N. and MORIWAKI, K. 81 VII. Population Genetics Further simulation studies on evolution by gene duplication. OHTA, T. 82 Time for acquiring a new gene by duplication. OHTA, T. 83 Evolution by gene duplication and compensatory advantageous
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