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Gold T. Rotating Neutron Stars As the Origin of the Pulsating Radio Sources
CC/NUMBER 8 ® FEBRUARY 22, 1993 This Week's Citation Classic Gold T. Rotating neutron stars as the origin of the pulsating radio sources. Nature 218:731-2, 1968; and Gold T. Rotating neutron stars and the nature of pulsars. Nature 221:25-7, 1969. [Center for Radiophysics and Space Research: and Cornell-Sydney University Astronomy Center. Cornell University, Ithaca. NY] Enigmatic, rapidly pulsating radio sources were preferred to attribute these sources to interpreted as rotating beacons sweeping over distant galaxies, not to stellar objects. the Earth, radiating out from rapidly rotating, The pulsars now seemed to repre- extremely collapsed stars ("neutron stars"). Nu- merous detailed predictions followed from this sent just the stellar objects I had dis- model, and all were soon verified. The model cussed then. Calculations existed for also accounted closely for the previously unex- the collapsed "neutron stars" that in- plained luminosity of the Crab Nebula. [The dicated approximately their size, as SCI® indicates that these papers have been small as a few kilometers, and their cited in more than 240 and 150 publications, mass, on the order of a solar mass.4 respectively.] Astronomers generally thought that even if they existed, they could never be discovered. However hot, a star so small could not be seen at astronomi- The Nature of Pulsars cal distances. But they had not con- sidered the energy concentration re- Thomas Gold sulting from the collapse: enormous Center for Radiophysics magnetic field strengths and a spin and Space Research energy quite comparable with the en- Cornell University tire content of nuclear energy of the Ithaca, NY 14853 star before its collapse. -
The Deep, Hot Biosphere (Geochemistry/Planetology) THOMAS GOLD Cornell University, Ithaca, NY 14853 Contributed by Thomas Gold, March 13, 1992
Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6045-6049, July 1992 Microbiology The deep, hot biosphere (geochemistry/planetology) THOMAS GOLD Cornell University, Ithaca, NY 14853 Contributed by Thomas Gold, March 13, 1992 ABSTRACT There are strong indications that microbial gasification. As liquids, gases, and solids make new contacts, life is widespread at depth in the crust ofthe Earth, just as such chemical processes can take place that represent, in general, life has been identified in numerous ocean vents. This life is not an approach to a lower chemical energy condition. Some of dependent on solar energy and photosynthesis for its primary the energy so liberated will increase the heating of the energy supply, and it is essentially independent of the surface locality, and this in turn will liberate more fluids there and so circumstances. Its energy supply comes from chemical sources, accelerate the processes that release more heat. Hot regions due to fluids that migrate upward from deeper levels in the will become hotter, and chemical activity will be further Earth. In mass and volume it may be comparable with all stimulated there. This may contribute to, or account for, the surface life. Such microbial life may account for the presence active and hot regions in the Earth's crust that are so sharply of biological molecules in all carbonaceous materials in the defined. outer crust, and the inference that these materials must have Where such liquids or gases stream up to higher levels into derived from biological deposits accumulated at the surface is different chemical surroundings, they will continue to repre- therefore not necessarily valid. -
And Thermo-Adaptation in Hyperthermophilic Archaea: Identification of Compatible Solutes, Accumulation Profiles, and Biosynthetic Routes in Archaeoglobus Spp
Universidade Nova de Lisboa Osmo- andInstituto thermo de Tecnologia-adaptation Química e Biológica in hyperthermophilic Archaea: Subtitle Subtitle Luís Pedro Gafeira Gonçalves Osmo- and thermo-adaptation in hyperthermophilic Archaea: identification of compatible solutes, accumulation profiles, and biosynthetic routes in Archaeoglobus spp. OH OH OH CDP c c c - CMP O O - PPi O3P P CTP O O O OH OH OH OH OH OH O- C C C O P O O P i Dissertation presented to obtain the Ph.D degree in BiochemistryO O- Instituto de Tecnologia Química e Biológica | Universidade Nova de LisboaP OH O O OH OH OH Oeiras, Luís Pedro Gafeira Gonçalves January, 2008 2008 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica Osmo- and thermo-adaptation in hyperthermophilic Archaea: identification of compatible solutes, accumulation profiles, and biosynthetic routes in Archaeoglobus spp. This dissertation was presented to obtain a Ph. D. degree in Biochemistry at the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa. By Luís Pedro Gafeira Gonçalves Supervised by Prof. Dr. Helena Santos Oeiras, January, 2008 Apoio financeiro da Fundação para a Ciência e Tecnologia (POCI 2010 – Formação Avançada para a Ciência – Medida IV.3) e FSE no âmbito do Quadro Comunitário de apoio, Bolsa de Doutoramento com a referência SFRH / BD / 5076 / 2001. ii ACKNOWNLEDGMENTS The work presented in this thesis, would not have been possible without the help, in terms of time and knowledge, of many people, to whom I am extremely grateful. Firstly and mostly, I need to thank my supervisor, Prof. Helena Santos, for her way of thinking science, her knowledge, her rigorous criticism, and her commitment to science. -
The Crystal Structure of Pyrococcus Furiosus Ornithine Carbamoyltransferase Reveals a Key Role for Oligomerization in Enzyme Stability at Extremely High Temperatures
Proc. Natl. Acad. Sci. USA Vol. 95, pp. 2801–2806, March 1998 Biochemistry The crystal structure of Pyrococcus furiosus ornithine carbamoyltransferase reveals a key role for oligomerization in enzyme stability at extremely high temperatures VINCENT VILLERET*, BERNARD CLANTIN†,CATHERINE TRICOT‡,CHRISTIANNE LEGRAIN‡,MARTINE ROOVERS§¶, i VICTOR STALON†,NICOLAS GLANSDORFF‡§¶, AND JOZEF VAN BEEUMEN* *Laboratorium voor Eiwitbiochemie en Eiwitengineering, Universiteit Gent, Ledeganckstraat 35, B-9000 Gent, Belgium; and †Laboratoire de Microbiologie, Universite´Libre de Bruxelles, ‡Institut de Recherches du Centre d’Enseignement et de Recherches des Industries Alimentaires, Commission de la Communaute´ Franc¸aise de Belgique, Re´gionBruxelles Capitale, §Laboratorium voor Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, and ¶Vlaams Interuniversitair Instituut voor Biotechnologie, avenue E. Gryson 1, B-1070 Brussels, Belgium Edited by Max F. Perutz, Medical Research Council, Cambridge, United Kingdom, and approved January 5, 1998 (received for review September 8, 1997) ABSTRACT The Pyrococcus furiosus (PF) ornithine car- lating agent (8). The involvement of such a thermolabile bamoyltransferase (OTCase; EC 2.1.3.3) is an extremely heat- intermediate in the metabolism of extreme thermophilic mi- stable enzyme that maintains about 50% of its activity after croorganisms raises the question of which mechanisms protect heat treatment for 60 min at 100°C. To understand the it from decomposition at elevated growth temperatures. Re- molecular basis of thermostability of this enzyme, we have cent results suggest that in Thermus aquaticus and Pyrococcus determined its three-dimensional structure at a resolution of furiosus (PF), CP is protected from the bulk of the aqueous 2.7 Å and compared it with the previously reported structures phase by channeling between carbamoylphosphate synthetase of OTCases isolated from mesophilic bacteria. -
Science Loses Some Friends Francis Crick, Thomas Gold, and Philip Abelson
Monthly Planet October 2004 Science Loses Some Friends Francis Crick, Thomas Gold, and Philip Abelson By Iain Murray he scientifi c world lost three impor- Throughout his career, Abelson used which suggests that the universe and Ttant fi gures in recent weeks, as scientifi c principles to determine genu- the laws of physics have always existed Francis Crick, Thomas Gold, and Philip ine new developments from hype and in the same, steady state. This has since Abelson have all passed away. In their publicity stunts (he was famously dis- been supplanted as the dominant cos- careers, each demonstrated the best that missive of the scientifi c value of the race mological paradigm by the Big Bang science has to offer humanity. Their loss to the moon). In that, he should prove a theory. illustrates how much worse off the state role model for true scientists. After working at the Royal Greenwich of science is today than during their Observatory, Gold moved to the United glory years. Thomas Gold States to become Professor of Astron- Astronomer Thomas Gold had an omy at Harvard. From there he moved Philip Abelson equally distinguished career, in fi elds as to Cornell, where he demonstrated that Philip Abelson was a scientist of truly diverse as engineering, physiology, and the newly discovered “pulsar” phenom- broad talents. One of America’s fi rst cosmology; and he was never afraid of enon must contain a rotating neutron nuclear physicists, he discovered the being called a maverick. A fellow of both star (a star more massive than the sun element Neptunium and designed the the Royal Society and the National Acad- but just 10 km in diameter). -
Notes of a Fringe -Watcher
MARTIN GARDNER Notes of a Fringe -Watcher The Anomalies of Chip Arp We have watched the farther galaxies I don't know what the 'C stands for, but fleeing away from us, wild herds of panic all his friends call him 'Chip.' He's in horses—or a trick of distance deceived danger of losing all those friends, if he the prism. keeps up what he's doing now! "Now mind you, I am kidding, be —Robinson Jeffers cause I am very fond of Chip Arp. In fact, we were graduate students at Caltech N HIS SPLENDID book The Drama together. But in those years he was nice." I of the Universe (1978) the late George An astronomer at Hale Observatories, Abell, an astronomer who served on the in California, Chip Arp is still adding to editorial board of this magazine, specu his collection of crazy objects, and still lates what generates the enormous ener making sharp rapier jabs at his more gies emitted by quasars (quasi-stellar orthodox colleagues. (Actual sword fenc sources). Quasars are almost surely the ing, by the way, is one of his major hob most distant known objects in the uni bies.) Like Thomas Gold, the subject of verse. Their gigantic redshifts indicate this column two issues back, Arp is a that some are moving outward at veloci competent, well-informed scientist who ties close to 90 percent of the speed of delights in the role of gadfly. His peculiar light. They occupy positions believed to anomalies are quasars that seem to be be very near the "edge" of the light connected by bridges of luminous gas barrier, a boundary beyond which noth but that have markedly different red- ing could ever be observed from our shifts, or high-redshift quasars that ap galaxy. -
Counts Metabolic Yr10.Pdf
Advanced Review Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms James A. Counts,1 Benjamin M. Zeldes,1 Laura L. Lee,1 Christopher T. Straub,1 Michael W.W. Adams2 and Robert M. Kelly1* The current upper thermal limit for life as we know it is approximately 120C. Microorganisms that grow optimally at temperatures of 75C and above are usu- ally referred to as ‘extreme thermophiles’ and include both bacteria and archaea. For over a century, there has been great scientific curiosity in the basic tenets that support life in thermal biotopes on earth and potentially on other solar bodies. Extreme thermophiles can be aerobes, anaerobes, autotrophs, hetero- trophs, or chemolithotrophs, and are found in diverse environments including shallow marine fissures, deep sea hydrothermal vents, terrestrial hot springs— basically, anywhere there is hot water. Initial efforts to study extreme thermo- philes faced challenges with their isolation from difficult to access locales, pro- blems with their cultivation in laboratories, and lack of molecular tools. Fortunately, because of their relatively small genomes, many extreme thermo- philes were among the first organisms to be sequenced, thereby opening up the application of systems biology-based methods to probe their unique physiologi- cal, metabolic and biotechnological features. The bacterial genera Caldicellulosir- uptor, Thermotoga and Thermus, and the archaea belonging to the orders Thermococcales and Sulfolobales, are among the most studied extreme thermo- philes to date. The recent emergence of genetic tools for many of these organ- isms provides the opportunity to move beyond basic discovery and manipulation to biotechnologically relevant applications of metabolic engineering. -
Ts2631 Endolysin from the Extremophilic Thermus Scotoductus Bacteriophage Vb Tsc2631 As an Antimicrobial Agent Against Gram-Negative Multidrug-Resistant Bacteria
viruses Article Ts2631 Endolysin from the Extremophilic Thermus scotoductus Bacteriophage vB_Tsc2631 as an Antimicrobial Agent against Gram-Negative Multidrug-Resistant Bacteria Magdalena Plotka 1,* , Malgorzata Kapusta 2, Sebastian Dorawa 1, Anna-Karina Kaczorowska 3 and Tadeusz Kaczorowski 1,* 1 Laboratory of Extremophiles Biology, Department of Microbiology, Faculty of Biology, University of Gdansk, 80-822 Gdansk, Poland 2 Department of Plant Cytology and Embryology, Faculty of Biology, University of Gdansk, 80-308 Gdansk, Poland 3 Collection of Plasmids and Microorganisms, Faculty of Biology, University of Gdansk, 80-308 Gdansk, Poland * Correspondence: [email protected] (M.P.); [email protected] (T.K.); Tel.: +48-58-523-60-75 (M.P.); +48-58-523-60-67 (T.K.) Received: 5 June 2019; Accepted: 15 July 2019; Published: 18 July 2019 Abstract: Bacteria that thrive in extreme conditions and the bacteriophages that infect them are sources of valuable enzymes resistant to denaturation at high temperatures. Many of these heat-stable proteins are useful for biotechnological applications; nevertheless, none have been utilized as antibacterial agents. Here, we demonstrate the bactericidal potential of Ts2631 endolysin from the extremophilic bacteriophage vB_Tsc2631, which infects Thermus scotoductus, against the alarming multidrug-resistant clinical strains of Acinetobacter baumannii, Pseudomonas aeruginosa and pathogens from the Enterobacteriaceae family. A 2–3.7 log reduction in the bacterial load was observed in antibacterial tests against A. baumannii and P. aeruginosa after 1.5 h. The Ts2631 activity was further enhanced by ethylenediaminetetraacetic acid (EDTA), a metal ion chelator (4.2 log reduction in carbapenem-resistant A. baumannii) and, to a lesser extent, by malic acid and citric acid (2.9 and 3.3 log reductions, respectively). -
BIOGEOCHEMICAL INTERACTIONS in FLOODED UNDERGROUND MINES Renee Schmidt Montana Tech
Montana Tech Library Digital Commons @ Montana Tech Graduate Theses & Non-Theses Student Scholarship Summer 2017 BIOGEOCHEMICAL INTERACTIONS IN FLOODED UNDERGROUND MINES Renee Schmidt Montana Tech Follow this and additional works at: http://digitalcommons.mtech.edu/grad_rsch Part of the Geochemistry Commons Recommended Citation Schmidt, Renee, "BIOGEOCHEMICAL INTERACTIONS IN FLOODED UNDERGROUND MINES" (2017). Graduate Theses & Non-Theses. 129. http://digitalcommons.mtech.edu/grad_rsch/129 This Thesis is brought to you for free and open access by the Student Scholarship at Digital Commons @ Montana Tech. It has been accepted for inclusion in Graduate Theses & Non-Theses by an authorized administrator of Digital Commons @ Montana Tech. For more information, please contact [email protected]. BIOGEOCHEMICAL INTERACTIONS IN FLOODED UNDERGROUND MINES by Renée Schmidt A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Geoscience: Geochemistry Option Montana Tech 2017 ii Abstract This study presents a biogeochemical analysis of microbial communities in flooded underground mines in Butte, Montana, USA. Samples were collected from nine mineshafts representing three distinct geochemical zones. These zones consist of the East, West, and Outer Camp mines. The East Camp mines, bordering the Berkeley Pit Superfund site, have the highest concentrations of dissolved metals and the most acidic pH values. Dissolved metal concentrations in the West Camp are one to three orders of magnitude lower than in the East Camp and have nearly neutral pH values. The Outer Camp mines have similar metal concentrations to the West Camp but are neutral to alkaline in pH. Sulfide levels also differ between the zones. In the East Camp, sulfide levels were below detection limits, whereas the West and Outer Camp mines had sulfide -6 -4 18 concentrations ranging from 10 to 10 mol/L. -
Can High Pressures Enhance the Long-Term Survival of Microorganisms?
Can High Pressures Enhance The Long-Term Survival of Microorganisms? Robert M. Hazen & Matt Schrenk Carnegie Institution & NASA Astrobiology Institute Pardee Keynote Symposium: Evidence for Long-Term Survival of Microorganisms and Preservation of DNA Philadelphia – October 24, 2006 Objectives I. Review the nature and distribution of life at high pressures. II. Examine the extreme pressure limits of cellular life. III. Compare experimental approaches to study microbial behavior at high P and T. IV. Explore how biomolecules and their reactions might change at high pressure? I. High-Pressure Life HMS Challenger – 1870s 20 MPa (~200 atmospheres) High-Pressure Life Deep-Sea Hydrothermal Vents – 1977 High-Pressure Life Discoveries of Microbial Life in Crustal Rocks – 1990s P ~ 100 MPa Witwatersrand Deep Microbiology Project Energy from radiolytic cleavage of water. Lin, Onstott et al. (2006) Science Thomas Gold’s Hypothesis: Organic Synthesis in the Mantle ß>300 MPa Thomas Gold (1999) NY:Springer-Verlag. Life at High Pressures on other Worlds? Deep, wet environments may exist on Mars, Europa, etc. I. What is the Nature and Distribution of Life at High-Pressure? Deep life, especially microbial life, is abundant throughout the upper crust . II. What are the Extreme Pressure Limits of Life? How deep might microbes exist in Earth’s crust? Pressure Can Enhance T Stability [Pledger et al. (1994) FEMS Microbiology [Margosch et al. (2006) Appl. Environ. Ecology 14, 233-242.] Microbiology 72, 3476-3481.] Life’s Extreme Pressure Limits 60 km Fast, cold slabs have regions of P > 2 GPa & T < 150°C [Stein & Stein (1996) AGU Monograph 96] Pressure Limits of Life Hydrothermal Opposed-Anvil Cell Pressure Limits of Life E. -
Edward Milne's Influence on Modern Cosmology
ANNALS OF SCIENCE, Vol. 63, No. 4, October 2006, 471Á481 Edward Milne’s Influence on Modern Cosmology THOMAS LEPELTIER Christ Church, University of Oxford, Oxford OX1 1DP, UK Received 25 October 2005. Revised paper accepted 23 March 2006 Summary During the 1930 and 1940s, the small world of cosmologists was buzzing with philosophical and methodological questions. The debate was stirred by Edward Milne’s cosmological model, which was deduced from general principles that had no link with observation. Milne’s approach was to have an important impact on the development of modern cosmology. But this article shows that it is an exaggeration to intimate, as some authors have done recently, that Milne’s rationalism went on to infiltrate the discipline. Contents 1. Introduction. .........................................471 2. Methodological and philosophical questions . ..................473 3. The outcome of the debate .................................476 1. Introduction In a series of articles, Niall Shanks, John Urani, and above all George Gale1 have analysed the debate stirred by Edward Milne’s cosmological model.2 Milne was a physicist we can define, at a philosophical level, as an ‘operationalist’, a ‘rationalist’ and a ‘hypothetico-deductivist’.3 The first term means that Milne considered only the observable entities of a theory to be real; this led him to reject the notions of curved space or space in expansion. The second term means that Milne tried to construct a 1 When we mention these authors without speaking of one in particular, we will use the expression ‘Gale and co.’ 2 George Gale, ‘Rationalist Programmes in Early Modern Cosmology’, The Astronomy Quarterly,8 (1991), 193Á218. -
Extremophiles
Extremophiles These microbes thrive under conditions that would kill other creatures. The molecules that enable extremophiles to prosper are becoming useful to industry by Michael T. Madigan and Barry L. Marrs DEEP-SEA VENT HEAT-LOVING MICROBES (THERMOPHILES AND HYPERTHERMOPHILES) SEA ICE COLD-LOVING MICROBES (PSYCHROPHILES) Methanopyrus kandleri Polaromonas vacuolata thereby increasing efficiency and reduc- magine diving into a refreshingly ing costs. They can also form the basis of cool swimming pool. Now, think entirely new enzyme-based processes. I instead of plowing into water that tially serve in an array of applications. Perhaps 20 research groups in the U.S., is boiling or near freezing. Or consider Of particular interest are the enzymes Japan, Germany and elsewhere are now jumping into vinegar, household am- (biological catalysts) that help extremo- actively searching for extremophiles and monia or concentrated brine. The leap philes to function in brutal circumstanc- their enzymes. Although only a few ex- would be disastrous for a person. Yet es. Like synthetic catalysts, enzymes, tremozymes have made their way into many microorganisms make their home which are proteins, speed up chemical use thus far, others are sure to follow. As in such forbidding environments. These reactions without being altered them- is true of standard enzymes, transform- microbes are called extremophiles be- selves. Last year the biomedical field and ing a newly isolated extremozyme into cause they thrive under conditions that, other industries worldwide spent more a viable product for industry can take from the human vantage, are clearly ex- than $2.5 billion on enzymes for appli- several years.