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15.0 Hazardous Waste Disposal
LABORATORY SAFETY MANUAL Revised June 2018 Emergency Contact Information In case of emergency: 911 Security Services: (daytime) 705-474-7600 ext. 5555 (cell phone) 705-498-7244 Laboratory Safety Coordinator: 474-3450 ext. 4180 Emergency Procedures** Chemical Spill On Body • Rinse affected area immediately for 15 minutes using emergency shower, if required. • Care must be taken to avoid contamination with face and eye(s). In Eye(s) • Immediately flush eye(s) using emergency eyewash station for a minimum of 15 minutes. In Laboratory • Assess the scene. If the situation is beyond your capabilities, contact your supervisor. • If safe to do so, turn off any ignition sources if flammable material is present. • Use spill kits to assist in spill containment. • Dispose of waste material with other hazardous waste. Fire • If you hear the Fire Alarm ring continuously evacuate the building immediately. • If you hear the Fire Alarm ring intermittently be alert however evacuation is not necessary. • If Fire Alarm changes from intermittent to continuous evacuate immediately • If you detect a fire, do not attempt to extinguish it yourself unless you are capable and it is safe to do so. Report the injury/incident to your supervisor as soon as possible. ** This is to be used as a quick reference only. For more detailed information, please refer to section 8.0 Emergency Procedures. Laboratory Safety Manual – Nipissing University 2 Table of Contents Acknowledgements ................................................................................................... -
Gas Generator Bottle Introduction SCIENTIFIC This Gas Generator Setup Provides an Easy Way to Generate and Collect Gas
Gas Generator Bottle Introduction SCIENTIFIC This gas generator setup provides an easy way to generate and collect gas. Specific instructions are provided for the generation of hydrogen gas using zinc and acid. Concepts • Generation of gases • Water displacement Materials Hydrochloric acid solution, HCl, 3 M Glass plates or Sulfuric acid solution, H2SO4, 3 M Glass tubing Mossy zinc, Zn, 6 g Pneumatic trough Water, tap Rubber tubing Bent glass tubing* Silicone grease packet* Gas collecting bottles or tubes, 3 or 4 Thistle tube* Gas generator bottle* Two-hole rubber stopper* *Materials included. Safety Precautions Hydrochloric acid solution is toxic by ingestion and inhalation and is severely corrosive to skin, eyes and other tissues, as is sulfuric acid solu- tion. Hydrogen gas is a highly flammable gas and a severe fire hazard. Exercise extreme caution when testing the gas and keep the gas generator away from flames. Wear chemical splash goggles, chemical-resistant gloves, and a chemical-resistant apron. This activity requires the use of hazardous components and/or has the potential for hazardous reactions. Please review current Material Safety Data Sheets for additional safety, handling, and disposal information. Procedure 1. Set up the apparatus as shown in the figure to the right. Lubricate the glass tubing and thistle tube with silicone grease before inserting into the stopper. Make sure Thistle tube the water level is above the platform. Prepare bottles for collecting gas by water Two-hole rubber stopper displacement. To do this, fill each gas collecting bottle (or tube) over the brim with tap water, and then cover each with a flat glass plate. -
Fisherbrand Reusable Glassware Brochure
Glassware Disposable, Reusable and Specialty Table of Contents Glassware Beakers ............................................................................................................................................................................................................... 1 Bottles ..............................................................................................................................................................................................................1-2 Media ........................................................................................................................................................................................................... 1 Storage .....................................................................................................................................................................................................1-2 Burets ...............................................................................................................................................................................................................2-3 Cells ..................................................................................................................................................................................................................4-5 Spectrophotometer ................................................................................................................................................................................. -
The Calibration of Small Volumetric Laboratory Glassware
NBSIR 74-461 The Calibration of Small Volumetric Laboratory Glassware Josephine Lembeck Institute for Basic Standards National Bureau of Standards Washington, D. C. 20234 December 1974 Final Prepared for Optical Physics Division Institute for Basic Standards Washington, D. C. 20234 NBSIR 74-461 THE CALIBRATION OF SMALL VOLUMETRIC LABORATORY GLASSWARE Josephine Lembeck Institute for Basic Standards National Bureau of Standards Washington, D. C. 20234 December 1974 Final Prepared for Optical Physics Division Institute for Basic Standards Washington, D. C. 20234 U. S. DEPARTMENT OF COMMERCE, Frederick B. Dent, Secretary NATIONAL BUREAU OF STANDARDS, Richard W. Roberts. Director The Calibration of Small Volumetric Laboratory Glassware by Josephine Lembeck 1. INTRODUCTION , This article describes an acceptable procedure for calibrating burets, pipets and volumetric flasks of the types shown in figure 1. The physical characteristics of these items are indicated in Interim Federal Specifications NNN-B-00789a (GSA-FSS) , NNN-P- 00395a (GSA-FSSi NNN-F-00289a (GSA-FSS), and NBS Handbook 105-2. The general procedure is based upon a determination of the volume of water either contained in or delivered by the vessel. This volume of water is based upon knowledge of its measured mass and its tabulated density. Inasmuch as these calibration volumes, especially the delivery volume, may be altered by the manner in which the vessels are used, it is imperative that the user of the vessel employ the same techniques as those employed during calibration. The techniques of importance here are: 1. reading and adjusting meniscus 2. filling the vessel 3. draining the vessel. v These three topics will be covered in detail. -
601 Automated Pipetting System Operation
™ EzMate 401 / 601 Automated Pipetting System Operation Manual Ver. 2.2 Trademarks: Beckman®, Biomek® 3000 ( Beckman Coulter Inc.); Roche®, LightCycler® 480 ( Roche Group); DyNAmo™ ( Finnzyme Oy); SYBR® (Molecular Probes Inc.); Invitrogen™, Platinum® ( Invitrogen Corp.), MicroAmp® ( Applied Biosystems); Microsoft® , Windows® 7 (Microsoft Corp.). All other trademarks are the sole property of their respective owners. Copyright Notice: No part of this manual may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, without prior written permission of Arise Biotech Corp. Notice: This product only provides pipetting functionality. Arise Biotech Corp. does not guarantee the performance and results of the assay and reagent. Table of Contents 1. Safety Precautions ............................................................................... 1 2. Product Introduction ........................................................................... 3 2.1 Features .................................................................................................................... 3 2.2 Hardware Overview ................................................................................................. 4 2.2.1 Outlook ........................................................................................................ 4 2.2.2 Control Notebook Computer........................................................................ 6 2.2.3 Automated Pipetting Module (APM).......................................................... -
Technical Methods an Interchangeable Throttling Device for Pipettes by J
J Clin Pathol: first published as 10.1136/jcp.7.4.348 on 1 November 1954. Downloaded from J. clin. Path. (19541, 7, 348. TECHNICAL METHODS AN INTERCHANGEABLE THROTTLING DEVICE FOR PIPETTES BY J. F. ACKROYD From the Medical Unit, St. Mary's Hospital Medical School, London (RECEIVED FOR PUBLICATION FEBRUARY 19, 1954) Many techniques in immunology require the tight plug of sealing-wax as shown in the figure. A rapid and accurate measurement of a large number rubber teat is then placed over the throttled end of the of small volumes of fluid. A day's work in a barrel. When a fine throttle is used expansion of the routine Wassermann laboratory doing 100 tests, compressed teat is so slow that the volume of fluid in can very controlled, for instance, involves the performance of nearly the graduated stem be accurately and small volumes of, for example, 0.1 ml. of fluid can 1,500 volumetric measurements. be discharged into a series of tubes with considerable Of the many devices for performing such measure- speed and accuracy. This design of pipette has three ments, one of the simplest and most satisfactory is the major drawbacks. First, the sealing-wax joints are liable throttled pipette shown in Fig. la. It is a slightly to fracture; second, the pipette cannot be cleaned without modified version of one described by Wright and Cole- melting the joints and subsequently reassembling the to brook in 1912, and has been in continuous use in the apparatus; and, third, the throttle tends become copyright. laboratories of St. -
Safety Handbook of the Department of Chemistry and Physics
SAFETY HANDBOOK OF THE DEPARTMENT OF CHEMISTRY AND PHYSICS Revised August 18, 2021 Approved by University Environmental Health and Safety Office August 18, 2021 Revised August 18, 2021 1 TABLE OF CONTENTS CONTENTS CONTENTS .................................................................................................................... 2 INTRODUCTION ............................................................................................................. 5 EMERGENCY RESPONSE NUMBERS ......................................................................... 7 RESPONSE TO ACCIDENTS ......................................................................................... 8 ACCIDENT/INCIDENT REPORT FORM ....................................................................... 10 SAFETY TRAINING ...................................................................................................... 11 SAFETY INSPECTIONS ............................................................................................... 11 REVIEW OF PROPOSED NEW EXPERIMENTS FOR TEACHING LABS ................... 11 SAFETY POLICY FOR LABORATORY WORK ............................................................ 12 SAFETY DOCUMENTATION PRIOR TO LABORATORY WORK ................................ 12 BUILDING EVACUATION ............................................................................................. 12 FIRE ALARM PROCEDURE ......................................................................................... 13 EMERGENCY RESPONSE TO LABORATORY FIRE ................................................. -
Safety in the Chemistry Laboratory
Reference: Safety in Academic Chemistry Laboratories-Accident Prevention for College and University Students. A Publication of American Chemical Society Joint Board-Council Committee on Chemical Safety. 7th Edition-Vol 1 Safety in the Chemistry Laboratory General • All students must pass the Safety Quiz and sign a Safety Agreement before working in the lab. • State and Federal law require the use of splash proof safety goggles by anyone working in a chemical lab. No student will be allowed to work in the lab or weighing room without wearing the department approved splash-proof safety goggles with side shield, lab apron/coat, and closed toe shoes. There will be no exception to this rule. • You will be doing lab experiments that require hazardous chemicals. To ensure a safe chemistry lab you need to follow : o all safety rules given , o the safety DVD, and o all written and verbal instructions given for each experiment. • All safety rules will be strictly enforced. Ignoring or failing to follow any safety rule or instruction will result in your being dismissed from the lab. Safety Equipment Know the locations/operations and use of the following emergency equipments: 1. Fire extinguisher is stored in a compartment attached to the wall. 2. Red fire alarm is on the wall at eyelevel next to the fire extinguisher 3. Fire blanket is stored inside a labeled red box attached to the wall next to the fire extinguisher. The blanket is to be used on clothing that caught fire. The blanket can also be used to cover a shock victim. 4. -
Laboratory Safety Manual – Nipissing University 2 Table of Contents Acknowledgements
LABORATORY SAFETY MANUAL Revised October 2015 Emergency Contact Information In case of emergency: 911 Security Services: (daytime) 474-7600 ext. 5555 (cell phone) 498-7244 Laboratory Safety Coordinator : 474-3450 ext. 4180 Manager, Environmental Health & Safety: 474-3450 ext. 4811 Emergency Procedures Chemical Spill On Body • Rinse affected area immediately for 15 minutes using emergency shower, if required. • Care must be taken to avoid contamination with face and eye(s). In Eye(s) • Immediately flush eye(s) using emergency eyewash station for a minimum of 15 minutes. In Laboratory • Assess the scene. If the situation is beyond your capabilities, contact your supervisor. • If safe to do so, turn off any ignition sources if flammable material is present. • Use spill kits to assist in spill containment. • Dispose of waste material with other hazardous waste. Fire • If you hear the Fire Alarm ring continuously evacuate the building immediately. • If you hear the Fire Alarm ring intermittently be alert however evacuation is not necessary. • If Fire Alarm changes from intermittent to continuous evacuate immediately • If you detect a fire, do not attempt to extinguish it yourself unless you are capable and it is safe to do so. Report the injury/incident to your supervisor as soon as possible. ** This is to be used as a quick reference only. For more detailed information, please refer to section 8.0 Emergency Procedures. Laboratory Safety Manual – Nipissing University 2 Table of Contents Acknowledgements ................................................................................................... -
Fisher Science Education 2021 Product Catalog Featured Suppliers
Lab Consumables Fisher Science Education 2021 Product Catalog Featured Suppliers Visit fisheredu.com/featuredsuppliers to learn more about these suppliers and their products. Helpful Icons Guarantee New product If you’re not 100% satisfied with your purchase, contact our customer service team within 30 days of your invoice date and we’ll either exchange, repair, or replace the product, or Must be shipped by truck for regulatory give you a credit for the full purchase price. Call us toll-free reasons for a return authorization number. Special order items, furniture, and closeouts cannot be exchanged or credited. Meets Americans with Disabilities Act Phone: 1-800-955-1177 • 7 a.m. to 5:30 p.m. requirements Central Time, Monday through Friday Fax: 1-800-955-0740 • 24 hours a day, 7 days a week Protects against splashes from Email: [email protected] hazardous chemicals or potentially infectious materials Website: fisheredu.com Address: Fisher Science Education 4500 Turnberry Drive Applicable for remote learning Hanover Park, IL 60133 For international orders, see page 110. All prices are subject to change. Connect with Us on Social Media fisheredu.com/facebook twitter.com/fishersciedu pinterest.com/fishersciedu Lab Consumables Preparing today’s students to be the innovators of tomorrow isn’t always easy, but finding the right teaching tools can be. From basic lab supplies to state-of- the-art classroom technology, the Fisher Science Education team has everything you need to create a 21st century STEM learning environment. Visit fisheredu.com to get started. Want to customize aspects of your curriculum? Explore custom kits to meet the unique demands of your classroom. -
Echo Inc. Glass Tube Thermometers Odor
Echo Inc: Thermometer Filling Safety Data Sheet Section 1: Identification Product Name: Echo Inc. glass tube thermometers Manufacturer/Supplier: Echo Inc. Address: 2755 Columbus Ave, Springfield, OH 45503 Emergency Phone Number: 855-639-3648 Product Use: Glass tube thermometers Section 2: Hazard(s) Identification Odor: No odor Immediate health, physical and environmental hazards: none Eye contact: No health effects are expected Skin contact: No health effects are expected Inhalation: No health effects are expected Ingestion: No health effects are expected Section 3: Composition/Information on Ingredients ' Ingredients C.A.S No. % byWt. Texanol Isobutyrate 6846-50-0 97-99 Solvent Blue A 14233-37-5 1-1.5 Solvent Red 26 4477-79-6 1-1.5 Toulene 108-88-3 < .25 Isopropanol 99% 67-63-0 < .25 , Section 4: First-Aid Measures First Aid Procedures: The following first aid recommendations are based on an assumption that appropriate personal and industrial hygiene practices are followed. Eye Contact: Flush eyes with water Skin contact: Wash affected area with soap and water Inhalation: No need for first air anticipated If swallowed: Make sure all glass is removed and rinse with water. Section 5: Fire-Fighting Measures Flammable Properties: Flash point: 289.99 Degrees Fahrenheit Extinguishing Media: Foam or Water but not directly on liquid, may cause frothing Protection of Fire Fighters: Wear full protective equipment (Bunker Gear) and a self-contained breathing apparatus. NFPA: Flammable and Combustible Liquids Classification: Combustible Liquid Class IliA Unusual Fire and Explosion Hazards: Not applicable Section 6: Accidental Release Measures Accidental Release Measures: Steps to be taken in case material is released or spilled. -
Pick-And-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation
micromachines Article Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation Moeto Nagai * ID , Keita Kato, Kiyotaka Oohara and Takayuki Shibata Department of Mechanical Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan; [email protected] (K.K.); [email protected] (K.O.); [email protected] (T.S.) * Correspondence: [email protected]; Tel.: +81-532-44-6701 Received: 22 October 2017; Accepted: 28 November 2017; Published: 30 November 2017 Abstract: A robust pick and placement operation of a single cell is necessary for efficient sample collection. Detection and manipulation of single cells requires minimum invasiveness. We report a less-invasive method for picking up and placing single cells using optical and electrical observations for robust cell manipulation. We measured the ionic current through a glass pipette during a cell capture and release operation to detect its capture. Trapping a cell on the pipette tip by suction decreased the current and allowed the detection of cell capture within 1 s. A time-series ionic current was sensitive to the location of a cell and effective at detecting a single cell. A time-series ionic current had a higher signal-to-noise ratio than time-series microscope images. Cell membrane integrity was analyzed at the different capturing and voltage conditions. Serum protein coating shows improvement of a cell release from a pipette tip. Measurement of trajectory and distance of a cell reveals that the movement depends on an ejection flow and the flow in a dish. We achieved a pick-up and placement operation for single cells that was compatible with an open-top microwell while performing observations using optical microscopy and measurements using an electrical current.