Pick-And-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation
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15.0 Hazardous Waste Disposal
LABORATORY SAFETY MANUAL Revised June 2018 Emergency Contact Information In case of emergency: 911 Security Services: (daytime) 705-474-7600 ext. 5555 (cell phone) 705-498-7244 Laboratory Safety Coordinator: 474-3450 ext. 4180 Emergency Procedures** Chemical Spill On Body • Rinse affected area immediately for 15 minutes using emergency shower, if required. • Care must be taken to avoid contamination with face and eye(s). In Eye(s) • Immediately flush eye(s) using emergency eyewash station for a minimum of 15 minutes. In Laboratory • Assess the scene. If the situation is beyond your capabilities, contact your supervisor. • If safe to do so, turn off any ignition sources if flammable material is present. • Use spill kits to assist in spill containment. • Dispose of waste material with other hazardous waste. Fire • If you hear the Fire Alarm ring continuously evacuate the building immediately. • If you hear the Fire Alarm ring intermittently be alert however evacuation is not necessary. • If Fire Alarm changes from intermittent to continuous evacuate immediately • If you detect a fire, do not attempt to extinguish it yourself unless you are capable and it is safe to do so. Report the injury/incident to your supervisor as soon as possible. ** This is to be used as a quick reference only. For more detailed information, please refer to section 8.0 Emergency Procedures. Laboratory Safety Manual – Nipissing University 2 Table of Contents Acknowledgements ................................................................................................... -
Gas Generator Bottle Introduction SCIENTIFIC This Gas Generator Setup Provides an Easy Way to Generate and Collect Gas
Gas Generator Bottle Introduction SCIENTIFIC This gas generator setup provides an easy way to generate and collect gas. Specific instructions are provided for the generation of hydrogen gas using zinc and acid. Concepts • Generation of gases • Water displacement Materials Hydrochloric acid solution, HCl, 3 M Glass plates or Sulfuric acid solution, H2SO4, 3 M Glass tubing Mossy zinc, Zn, 6 g Pneumatic trough Water, tap Rubber tubing Bent glass tubing* Silicone grease packet* Gas collecting bottles or tubes, 3 or 4 Thistle tube* Gas generator bottle* Two-hole rubber stopper* *Materials included. Safety Precautions Hydrochloric acid solution is toxic by ingestion and inhalation and is severely corrosive to skin, eyes and other tissues, as is sulfuric acid solu- tion. Hydrogen gas is a highly flammable gas and a severe fire hazard. Exercise extreme caution when testing the gas and keep the gas generator away from flames. Wear chemical splash goggles, chemical-resistant gloves, and a chemical-resistant apron. This activity requires the use of hazardous components and/or has the potential for hazardous reactions. Please review current Material Safety Data Sheets for additional safety, handling, and disposal information. Procedure 1. Set up the apparatus as shown in the figure to the right. Lubricate the glass tubing and thistle tube with silicone grease before inserting into the stopper. Make sure Thistle tube the water level is above the platform. Prepare bottles for collecting gas by water Two-hole rubber stopper displacement. To do this, fill each gas collecting bottle (or tube) over the brim with tap water, and then cover each with a flat glass plate. -
2017 Automated Liquid Handling Resource Guide
The Lab Manager AUTOMATED LIQUID HANDLING RESOURCE GUIDE What You Need to Know When Buying an Automated Liquid Handling System BY RYAN ACKERMAN Upgrading Stand-Alone Automated Liquid Handling Systems to Workstations BY ANGELO DEPALMA, PhD Small-Volume Liquid Handling Requires Advanced Technology BY MIKE MAY, PhD 20 10 Common Liquid Handling Errors & How to Avoid Them 17 BY LAB MANAGER The Latest in Pipette Tip Design BY ANGELO DEPALMA, PhD Automated Liquid Handling Product Finder BY LAB MANAGER Automatic Liquid Handling Resource Guide 2017 What You Need to Know When Buying an Automated Liquid Handling System Automated liquid handlers come in a seemingly endless variety of configurations, with many different specifications. By Ryan Ackerman MAINTENANCE TIP: AUTOMATED LIQUID HANDLING The signs that you should get your automated liquid handler serviced are fairly obvious. If the system is constantly experiencing glitches or producing inconsistent, unreliable results, it’s probably time to do some maintenance. Mechanical problems are another hint that you may want to call your service technician—these issues can include pipette tips being out of calibration, the deck not being “framed” correctly, belts being worn out, or the pipette stages being out of alignment. Why is it important to know the typical sample How does the sensitivity to contamination affect the type volume being used? of automated liquid handler required? Automated liquid handlers come in a seemingly endless variety of As the instrumentation used to analyze samples becomes more configurations, with many different specifications. An important one sensitive, the methods used to transport and prepare the samples to consider is what minimum, or maximum dispensing volume is must become resistant to contamination to ensure no carryover correct for your processes. -
Fisherbrand Reusable Glassware Brochure
Glassware Disposable, Reusable and Specialty Table of Contents Glassware Beakers ............................................................................................................................................................................................................... 1 Bottles ..............................................................................................................................................................................................................1-2 Media ........................................................................................................................................................................................................... 1 Storage .....................................................................................................................................................................................................1-2 Burets ...............................................................................................................................................................................................................2-3 Cells ..................................................................................................................................................................................................................4-5 Spectrophotometer ................................................................................................................................................................................. -
Q & a – Accuracy and Precision
Food Analysis – FScN 4312W Laboratory: Assessment of Accuracy and Precision Key to Questions 1. Theoretically, how are standard deviation, coefficient of variation, mean, percent relative error, and 95% confidence interval affected by: (1) more replicates, and (2) a larger size of measurement? Was this evident in looking at the actual results obtained using the volumetric pipettes and the buret, with n = 3 vs n = 6, and with 1mL vs 10mL? Ans: (1) As the sample size increases (more replicates) - Calculated mean approaches the true mean - Standard deviation is inversely proportional to the square root of sample size, hence it decreases - CV decreases, as standard deviation approaches 0 for larger sample size - % Relative error decreases as calculated mean approaches true mean - 95% confidence interval narrows down (2) Larger size of measurement - Mean is close to true mean - Standard deviation might be larger due to larger size - CV decreases - % Relative error decreases, as calculated mean is close to true mean - 95% confidence interval narrows down Not all of the above assumptions were confirmed in this experiment according to the data observed. One reason could be that the sample size was very small (n = 3 and n = 6), to notice significant differences, another reason could be human error and variation from one person’s measuring technique to another. 2. Why are percent relative error and coefficient of variation used to compare the accuracy and precision, respectively, of the volumes from pipetting/dispensing 1 and 10mL with the volumetric pipettes and buret in parts 2 and 3, rather than simply the mean and standard deviation, respectively? Ans: Mean calculated from the readings gives the calculated mean, which may differ from the true mean. -
Introducing Automation to Your Lab
Introducing Automation to Your Lab A step-by-step reference guide for the 21st Century biologist Written by Opentrons © OPENTRONS 2019 | INTRODUCING AUTOMATION TO YOUR LAB 1 INTRODUCTION Table of Contents CONTENT FIGURES Introduction Dispelling Myths About Lab Automation 03 Figure 1 Busting The Top 5 Automation Myths 03 Chapter 1 The Overall Benefits Of Lab Automation 05 Figure 2 Top 5 Benefits Of Lab Automation 05 Chapter 2 Choosing The Best Workflow To Automate 06 Figure 3 Your Workflow Is A Good Candidate For 06 Automation If... Chapter 3 Precision And Lab Automation 08 Figure 4 Translating Manual Processes To Automation 07 Chapter 4 How Much Liquid To Move? 09 – Volume Ranges Figure 5 Levels of Throughput 09 – Low, Medium, or High Throughput? Figure 6 Robot Pricing Table 12 Chapter 5 Space Considerations 10 Figure 7 The Opentrons Approach: 14 – Sterility Figure 8 Next Steps When Purchasing An Automated 16 – Size Lab Robot Chapter 6 Cash Considerations 11 Chapter 7 Developing Your Protocol 14 Chapter 8 Choosing The Right Vendor 15 Conclusion Next Steps On The Path To Automation 16 Appendix Resources 17 © OPENTRONS 2019 | INTRODUCING AUTOMATION TO YOUR LAB 2 INTRODUCTION Dispelling the Myths of Lab Automation “Repetitio est mater studiorum,” says the Latin FIGURE 1 proverb: “Repetition is the mother of all learning.” Busting the top 5 automation myths Scientists live this ancient saying every day, winning hard-earned discoveries by repeating the same process over and over. In some cases, this simply MYTH REALITY builds a large enough sample size to create statistical significance in the results. -
The Calibration of Small Volumetric Laboratory Glassware
NBSIR 74-461 The Calibration of Small Volumetric Laboratory Glassware Josephine Lembeck Institute for Basic Standards National Bureau of Standards Washington, D. C. 20234 December 1974 Final Prepared for Optical Physics Division Institute for Basic Standards Washington, D. C. 20234 NBSIR 74-461 THE CALIBRATION OF SMALL VOLUMETRIC LABORATORY GLASSWARE Josephine Lembeck Institute for Basic Standards National Bureau of Standards Washington, D. C. 20234 December 1974 Final Prepared for Optical Physics Division Institute for Basic Standards Washington, D. C. 20234 U. S. DEPARTMENT OF COMMERCE, Frederick B. Dent, Secretary NATIONAL BUREAU OF STANDARDS, Richard W. Roberts. Director The Calibration of Small Volumetric Laboratory Glassware by Josephine Lembeck 1. INTRODUCTION , This article describes an acceptable procedure for calibrating burets, pipets and volumetric flasks of the types shown in figure 1. The physical characteristics of these items are indicated in Interim Federal Specifications NNN-B-00789a (GSA-FSS) , NNN-P- 00395a (GSA-FSSi NNN-F-00289a (GSA-FSS), and NBS Handbook 105-2. The general procedure is based upon a determination of the volume of water either contained in or delivered by the vessel. This volume of water is based upon knowledge of its measured mass and its tabulated density. Inasmuch as these calibration volumes, especially the delivery volume, may be altered by the manner in which the vessels are used, it is imperative that the user of the vessel employ the same techniques as those employed during calibration. The techniques of importance here are: 1. reading and adjusting meniscus 2. filling the vessel 3. draining the vessel. v These three topics will be covered in detail. -
2021 Product Guide
2021 PRODUCT GUIDE | LIQUID HANDLING | PURIFICATION | EXTRACTION | SERVICES TABLE OF CONTENTS 2 | ABOUT GILSON 56 | FRACTION COLLECTORS 4 | COVID-19 Solutions 56 | Fraction Collector FC 203B 6 | Service Experts Ready to Help 57 | Fraction Collector FC 204 7 | Services & Support 8 | OEM Capabilities 58 | AUTOMATED LIQUID HANDLERS 58 | Liquid Handler Overview/selection Guide 10 | LIQUID HANDLING 59 | GX-271 Liquid Handler 11 | Pipette Selection Guide 12 | Pipette Families 60 | PUMPS 14 | TRACKMAN® Connected 60 | Pumps Overview/Selection Guide 16 | PIPETMAN® M Connected 61 | VERITY® 3011 18 | PIPETMAN® M 62 | Sample Loading System/Selection Guide 20 | PIPETMAN® L 63 | VERITY® 4120 22 | PIPETMAN® G 64 | DETECTORS 24 | PIPETMAN® Classic 26 | PIPETMAN® Fixed Models 66 | PURIFICATION 28 | Pipette Accessories 67 | VERITY® CPC Lab 30 | PIPETMAN® DIAMOND Tips 68 | VERITY® CPC Process 34 | PIPETMAN® EXPERT Tips 70 | LC Purification Systems 36 | MICROMAN® E 71 | Gilson Glider Software 38 | DISTRIMAN® 72 | VERITY® Oligonucleotide Purification System 39 | REPET-TIPS 74 | Accessories Overview/Selection Guide 40 | MACROMAN® 75 | Racks 41 | Serological Pipettes 43 | PLATEMASTER® 76 | GEL PERMEATION 44 | PIPETMAX® CHROMATOGRAPHY (GPC) 76 | GPC Overview/Selection Guide 46 | BENCHTOP INSTRUMENTS 77 | VERITY® GPC Cleanup System 46 | Safe Aspiration Station & Kit 47 | DISPENSMAN® 78 | EXTRACTION 48 | TRACKMAN® 78 | Automated Extraction Overview/ 49 | Digital Dry Bath Series Selection Guide 49 | Roto-Mini Plus 80 | ASPEC® 274 System 50 | Mini Vortex Mixer 81 | ASPEC® PPM 50 | Vortex Mixer 82 | ASPEC® SPE Cartridges 51 | Digital Mini Incubator 84 | Gilson SupaTop™ Syringe Filters 86 | EXTRACTMAN® 52 | CENTRIFUGES 52 | CENTRY™ 103 Minicentrifuge 88 | SOFTWARE 53 | CENTRY™ 117 Microcentrifuge 88 | Software Selection Guide 53 | CENTRY™ 101 Plate Centrifuge 54 | PERISTALTIC PUMP 54 | MINIPULS® 3 Pump & MINIPULS Tubing SHOP ONLINE WWW.GILSON.COM 1 ABOUT US Gilson is a family-owned global manufacturer of sample management and purification solutions for the life sciences industry. -
Laboratory Equipment Used in Filtration
KNOW YOUR LAB EQUIPMENTS Test tube A test tube, also known as a sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. Beakers Beakers are used as containers. They are available in a variety of sizes. Although they often possess volume markings, these are only rough estimates of the liquid volume. The markings are not necessarily accurate. Erlenmeyer flask Erlenmeyer flasks are often used as reaction vessels, particularly in titrations. As with beakers, the volume markings should not be considered accurate. Volumetric flask Volumetric flasks are used to measure and store solutions with a high degree of accuracy. These flasks generally possess a marking near the top that indicates the level at which the volume of the liquid is equal to the volume written on the outside of the flask. These devices are often used when solutions containing dissolved solids of known concentration are needed. Graduated cylinder Graduated cylinders are used to transfer liquids with a moderate degree of accuracy. Pipette Pipettes are used for transferring liquids with a fixed volume and quantity of liquid must be known to a high degree of accuracy. Graduated pipette These Pipettes are calibrated in the factory to release the desired quantity of liquid. Disposable pipette Disposable transfer. These Pipettes are made of plastic and are useful for transferring liquids dropwise. Burette Burettes are devices used typically in analytical, quantitative chemistry applications for measuring liquid solution. Differing from a pipette since the sample quantity delivered is changeable, graduated Burettes are used heavily in titration experiments. -
Thermo Scientific Pipetting Guide
Thermo Scientific Pipetting Guide Tips for Good Laboratory Pipetting Part of Thermo Fisher Scientific Thermo Scientific Thermo Scientific Contents Introduction 3 Pipetting Pipetting Guide Pipetting terms 3 Types of pipettes 4 General pipetting guidelines 6 Pipetting techniques 6 Tip information 7 Recommendations for pipetting different compounds 8 Ensuring optimum performance 8 Usage of Finpipette Novus 9 Factors affecting the accuracy of air displacement pipettes 10 Preventing cross-contamination 11 Maintenance of your Finnpipettes 1 Autoclaving 1 UV resistance 1 Calibrating your pipettes (incl. conversion table) 13 On-line pipette calibration 15 General guidelines for decontaminating pipettes 16 Chemical compatibility of plastics 18 Customer support 19 Trouble shooting 19 Thermo Scientific Over 35 Years of Innovation A leader in pipetting For over 35 years we have led the way in liquid handling products and microplate instrumentation. We have always ensured that innovation, ergonomics, accuracy, precision and safety are key aspects of our products’ designs. In 1971 we introduced Thermo Scientific Finnpipettes, the world’s first continuously variable micropi- pettes. In 1976 we introduced the world’s first multichannel pipette. Since then we have continuously improved our pipettes, always leading the way with ergonomic design. Over the last 35 years, innovations such as the improved finger rest, soft-touch tip ejection and super blow-out have made Finnpipettes increasingly user-friendly. Our intensive research program and commitment to our customers forms the foundation for future innovations. The new demands in pipette applications are the key drivers of our product development. To date, over 3 mil- lion Finnpipettes have been sold in 150 countries. -
96-Pcr Plate Domino
DOMI N Y O B B Y O N D O M I PIPETTE CONSUMABLE COMPATIBILITY OneLab 1.7.0 PIPETTE | CONSUMABLE COMPATIBILITY OneLab 1.7.0 OneLab Design & Execute Unique software for the design and execution of lab protocols. The pace of innovation in current day biological research demands the highest levels of reproducibility and traceability. Andrew Alliance, a Waters Company, has developed an intelligent cloud-based software environment called OneLab within which experiments can be intuitively designed, repeatably executed, and tracked through a rapidly evolving ecosystem of connected devices and accessories that it is building together with partner organisations. Andrew Alliance has developed the Andrew+ Pipetting Robot and the Pipette+ Intelligent Pipetting System, both of which seamlessly connect with OneLab. Andrew Alliance is an eco-friendly CO2-Neutral Certified Company, dedicated to developing sustainable solutions. Andrew Alliance is constantly adding new capabilities, accessories and consumables to OneLab as indicated by this catalog which is updated on a regular basis. 1 500 Compatible Incompatible « on-the-fly » : Numeric value : compatible only with minimum volume in uL on-the-fly dispensing which must be present in the consumable to avoid collisions. 2 /83 PIPETTE | CONSUMABLE COMPATIBILITY OneLab 1.7.0 [218.2002] MICROPLATE DOMINO CELLSTAR® 24-well cell culture plate, Greiner Bio-One CELLSTAR® 24-well cell culture multiwell plate; Flat well bottom; Chimney well design; Each well has a fl attened, raised ring to re- duce cross contamination; -
601 Automated Pipetting System Operation
™ EzMate 401 / 601 Automated Pipetting System Operation Manual Ver. 2.2 Trademarks: Beckman®, Biomek® 3000 ( Beckman Coulter Inc.); Roche®, LightCycler® 480 ( Roche Group); DyNAmo™ ( Finnzyme Oy); SYBR® (Molecular Probes Inc.); Invitrogen™, Platinum® ( Invitrogen Corp.), MicroAmp® ( Applied Biosystems); Microsoft® , Windows® 7 (Microsoft Corp.). All other trademarks are the sole property of their respective owners. Copyright Notice: No part of this manual may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, without prior written permission of Arise Biotech Corp. Notice: This product only provides pipetting functionality. Arise Biotech Corp. does not guarantee the performance and results of the assay and reagent. Table of Contents 1. Safety Precautions ............................................................................... 1 2. Product Introduction ........................................................................... 3 2.1 Features .................................................................................................................... 3 2.2 Hardware Overview ................................................................................................. 4 2.2.1 Outlook ........................................................................................................ 4 2.2.2 Control Notebook Computer........................................................................ 6 2.2.3 Automated Pipetting Module (APM)..........................................................