SINAI Journal of Applied Sciences 10 (1) 2021 039-046 Available online at www.sinjas.journals.ekb.eg SINAI Journal of Applied Sciences

Print ISSN 2314-6079 Online ISSN 2682-3527 IDENTIFICATION AND TAXONOMIC STUDY OF SHRIMPS IN BARDAWIL LAGOON, NORTH SINAI, Samar A. Amin1*, G.D.I. Hassanen2 and M.S. Ahmed3 1. Post-Grad. Stud., Dept. Fish Res., Fac. Environ. Agric. Sci., Arish Univ., Egypt. 2. Dept. Fish Res., Fac. Environ. Agric. Sci., Arish Univ., Egypt. 3. Fac. Aquacul. and Marine Fisheries, Arish Univ., Egypt. ARTICLE INFO ABSTRACT Article history: Taxonomic study of shrimp on the Bardawil lagoon, were studied in Received: 14/03/2021 specimens of shrimp collected from Bardawil lagoon fishers from Seasonal Revised: 19/03/2021 occurrence of shrimp During, 2019. Identification of species was based on the Accepted: 01/04/2021 morphological characters of rostrum (dorsal and ventral teeth), remarks of Available online: 13/04/2021 carapace, antenna, sub apical spines on telson and the colored pattern of the Keywords: whole body based on standard keys and diagnoses available from the current Species, literature. Modified Key identify shrimp species from Bardawil lagoon, was Shrimps, prepared after morphometric analysis. A total of 5 species from belong to Taxonomic, three ; , and . Among these, two Bardawil lagoon species belong to the genus Penaeus ( (De Haan, 1844) and Penaeus () japonicas (Bate, 1888). The species which taken from genus Metapenaeus namely Metapenaeus stebbingi (Nobili, 1904) and . (Fabricus, 1798), One species belong to the genus . (Forskål, 1775). Additional research is needed Check for to more clearly define the distribution of shrimp species in Bardawil lagoon. updates

INTRODUCTION MATERIALS AND METHODS

Penaeid shrimps are important resources Bardawil lagoon (Fig. 1) is a shallow lagoon of high salinity. It’s area about 685 for worldwide fisheries and . 2 They supply the increasing population with km (165,000 acres) and extends for a distance of about 95 km, from point 45 km east of proteins and provide significant Port Said to the point 18 km west of Al- earnings of foreign exchange for developing Arish and its maximum width is 22 km and countries (Jayawickrama, 2010). Without the average depth is 135 cm (Gafrd, 2009). , nobody would be sure of the identity of organisms whether they belonged Sampling and Morphological to the same or different species as the failure Identification to recognize fishes as distinct biological The study was carried out during the 3 units can lead to wrong diagnosis (Guerra- seasons (Summer- Spring-Autumn). The Garcia Without taxonomy, nobody would collection of 731 random samples of shrimp be sure of the identity of organisms whether species from the commercial catch of they belonged to the same or different Bardawil Lagoon, 2019. Which were iced in species as the failure to recognize fishes as an ice bin and transported to the laboratory distinct biological units can lead to wrong of the Institute of Environmental Studies, Arish University for taxonomic examinations diagnosis (Guerra-Garcia et al., 2008; and measurements. The present study was Keat-Chuan et al., 2017). This research is mainly based on the phenotype and meristic preliminary attempt to provide a detailed character observation of fresh specimens in identification of shrimps in Bardawil lagoon. the samples. ـــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ * Corresponding author: E-mail address: [email protected] https://doi.org/ 10.21608/SINJAS.2021.67830.1012 © 2020 SINAI Journal of Applied Sciences. Published by Fac. Environ. Agric. Sci., Arish Univ. All rights reserved.

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Fig. 1. Satellite image of Bardawil lagoon

The present study was mainly based on which taken from genus Metapenaeus the phenotype and meristic character namely Metapenaeus stebbingi and observation of fresh specimens in the Metapenaeus monoceros. One species samples. Shrimps were examined using belong to the genus Melicertus (Melicertus hand lenses and under a binocular stereo kerathurus). Morphometric characteristics microscope (OPTIKA Stereomicroscope showed more distinguish between MB5–4083.B5, ). Samples were individuals of Penaeus and Metapenaeus as determined at the species level and before the identification of shrimps traditionally Measurement, samples were separated on are relied on morphometric analysis; however, the basis of phenotype. The identity was it is well known that such characteristics are confirmed with the help of widely used influenced by environmental conditions references (De Bruin et al., 1994; Carpenter (Bowman et al., 1982). Piratheepa et al. and Niem, 1998 and Piratheepa et al., (2016), described Penaeus semisulcatus as 2016). having a reddish brown to pale brown body color may be Dark in color. The pulpit has RESULTS AND DISCUSSION 5-8 teeth on the top and 2-4 teeth on the ventral side. Based on the morphological Morphological Analyses and Shrimp characteristics, two groups of P. semisulcatus Species are distinguished in the Persian Gulf. The first group is characterized by a reddish In this study, a total of five species of body color with deep red or brown Penaeid shrimps were identified by transverse bands, cream and brown striped morphologically. Shrimp were determined color of the whip antenna. Second group based on the following morphological subspecies of P. persicus, is characterized characteristics. Number of rostral teeth, by a creamy pink body color without distinct down area telson and exopods on fifth transverse stripes, and its whip antenna has pereiopods were considered one of the a cream color without stripes (Rahnama et major morphological characteristics used to al., 2010). Otero et al. (2013), were identified identify shrimp species, which is categorized Marsupenaeus japonicus, result showed a under one family, the family. It well-developed rostrum with 7 - 11 teeth on became clear through the study, there are the dorsal margin and 1 tooth on the ventral five species belong to three genus; Penaeus, marginal. The body was pale, with brown Melicertus and Metapenaeus. Among these, bands and the pereiopods and pleopods two species belong to the genus Penaeus were pale yellow near their bases with blue (Penaeus semisulcatus and Penaeus markings near the tips. FAO (2011) describe (Marsupenaeus) japonicas). The species

Amin, et al. | SINAI Journal of Applied Sciences 10 (1) 2021 039-046 41 Melicertus kerathurus body is light creamy 7. Rostrum with one tooth on ventral side brown with gray spots and rust colored. …. Penaeus (Marsupenaeus) japonicas Carapace is Creamy. Rostrum has Mostly 9 and Melicertus kerathurus. teeth on the top and 1 tooth appear on the 8. Rostrum with 9–10 teeth on dorsal side, ventral side. There are 3 cicatrices on the pereiopods and pleopods yellow and blue last abdominal segments. Piratheepa et al. and 3 pairs of movable spines on telson. (2016) descriptive remarks for Metapenaeus Body with yellow and brown bands monoceros as follows body pink, antennae ……Penaeus (Marsupenaeus) japonicas. with a red Colour; pereopods and pleopods of same Colour as body, sometimes more 9. Rostrum with 8–13 teeth on dorsal side and there are spines on base of first 2 intensely pink. Carapace is pink with a pairs of pereiopods ………. Melicertus grayish dark spot. Rostrum has 9-12 teeth kerathurus. on the top and no tooth appear on the ventral side. The outer tip of the Telson is Shrimps were identified based on the blue. Jayawickrama (2010). Describe of morphological characteristics. Through Metapenaeus stebbingi. Body white to virtual examination and microscopic creamy–yellow with grey and rust coloured examination inside the laboratory, each specks; antennae Colour to grey–purplish. species was determined separately by The rostrum there is no tooth on ventral studying its phenotype as a follow: side and 7-10 teeth on the top. Telson with Penaeus semisulcatus (De Haan, 1844) spines. All of these results are in complete Common name agreement with the results of this thesis in describing their type in terms of Green tiger . appearance. Colour Key Identifies Shrimp Species from Pale reddish brown with dark brown Bardawil Lagoon dorsal transverse stripes. White and brown antenna. 1. Rostrum with teeth on its dorsal side only ...... Genus Metapenaeus. Descriptive remarks 2. Rostrum with 9–12 teeth on dorsal side, The carapace is reddish brown Colour telson with movable spines and there are with Brown transverse lines. The rostrum basial spines on first 3 pereiopods has 5-8 teeth on the top and 2-4teeth appear ……… Metapenaeus monoceros. on the ventral side. Antenna banded white and brown. 3. Rostrum with 8–10 teeth on dorsal side and no ischial spine on 1stpereiopod Penaeus (Marsupenaeus) japonicus …….. Metapenaeus stebbingi. (Spence Bate, 1888) Common name 4. Rostrum with teeth on its dorsal and ventral side………Genus Penaeus. Kuruma prawn 5. Rostrum with 2–4 teeth on ventral side Colour ………. Penaeus semisulcatus. Lightly brown to greenish brown with 6. Rostrum with 5–8 teeth on dorsal side, blue edge at tail fan. and no spines on telson and antennae Descriptive remarks banded white and brown. Dark green to The carapace is soft and shiny. The dark brown cross bands on the abdomen, rostrum has one tooth on ventral side and 9 leg with yellow and reddish bands ……. teeth on the top. Telson has a mixture of Penaeus semisulcatus. three colors brown, yellow and blue.

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Fig. 2. Penaeus semisulcatus from Bardawil lagoon during fishing season, 2019

Fig. 3. Penaeus (Marsupenaeus) japonicus from Bardawil lagoon during fishing season, 2019

Fig. 4. Melicertus kerathurus from Bardawil lagoon during fishing season, 2019

Melicertus kerathurus (Forskål, 1775) Descriptive remarks Colour The carapace is pink with a grayish dark spot. The rostrum has 9-12 teeth on the top The body is light creamy brown with and no tooth appear on the ventral side. The gray spots and rust colored. outer tip of the Telson is blue. Descriptive remarks Metapenaeus stebbingi (Nobili, 1904) The carapace is Creamy. The rostrum has 9 teeth on the top and 1 tooth appear on Common name the ventral side. There are 3 cicatrices on Peregrine shrimp the last abdominal segments. Colour Metapenaeus monoceros (Fabricus, 1798) White to creamy-yellow with grey Common name coloured specks and antennae by Colour grey. Ginger prawn. Descriptive remarks Colour The carapace is cream colour. The Body pink, antennae with a red Colour; rostrum has 7-10 teeth on the top and no pereopods and pleopods of same Colour as teeth appear on the ventral side. Telson body, sometimes more intensely pink. with a spine.

Amin, et al. | SINAI Journal of Applied Sciences 10 (1) 2021 039-046 43

Fig. 5. Metapenaeus monoceros from Bardawil lagoon during fishing season, 2019

Fig. 6. Metapenaeus stebbingi from Bardawil lagoon during fishing season, 2019

Conclusion 1873–76. Zool. 24 (part 52): i-xc, 1-942, 1-150. During the present study, Randomly collection of samples and transported for Bowman, T.E. and Abele, L.G. (1982). taxonomic examinations and measurements. Classification of recent Crustacea, In: The present study was mainly based on the L.G. Abele (Ed.). The Biology of the phenotype and meristic character observation Crustacea. Systematics, the Fossil of fresh specimens. and through the Record and Biogeography, Academic examination presence was recorded five Press, New York, 1-27. species Penaeus semisulcatus, Metapenaeus Brito, C. (2001). Towards an institutional stebbingi, Penaeus japonicus, Metapenaeus theory of the dynamics of industrial monoceros and Melicertus Keraturus in networks, J. Business and Indust. Bardawil lagoon in North Sinai and their Market., 16 (3): 150-166. identifying characters were very closely Charpenter, K.E. and Niem, V.H. (1998). similar with the descriptions given by Many FAO species identification guide for previous studies. fishery purposes. The living marine resources of The Western Central Pacific REFERENCES Vol. 2. Cephalopods, , Holothurians and Sharks. FAO, Rome, Abusaif, S.M. (2017). Biological and fishery 687-1396. studies on The Commercial Prawn Crocos, P.J. (1987). Reproductive dynamics species (Marsupenaeus japonicus) on of the tiger prawn Penaeus esculentus, the western Mediterranean Egyptian and a comparison with P. semisulcatus, coast. Marine Sci. Dept., Fac. Sci., Suez in the north-western Gulf of Carpentaria, Canal Univ. Australia. Marine and Freshwater Res., 38 (1): 91-102. Bate, C.S. (1888). Report on the Crustacea Macrura collected by the Challenger De Bruin, G.H.P.; Russell, B. and Bogusch, during the years 1873-76. Report on the A. (1994). The Marine Fishery Resources Scientific Results of the Voyage of of , FAO species Identification guide for fishery purposes. FAO, Rome. H.M.S. Challenger During the Years

44 Amin, et al. | SINAI Journal of Applied Sciences 10 (1) 2021 039-046 De Haan, W. (1844). Crustacea. In: von Salem, M. and El-Aiatt, A. (2012). Siebold, P.F., Fauna Japonica sive Improvement of Trammel Net Landing Descriptio Animalium, quae in Itinereper and Reduction of fishing for Gilthead Japoniam, Jussu et Auspiciis Superiorum, Sea bream (Sparus aurata) Juveniles in qui Summum in India Batava Imperium Bardawil lagoon, North Sinai, Egypt. J. Tenent, Suspecto, Annis 1823-1830 Animal, Poultry and Fish Prod., Suez Collegit, Notis, Observationibus et Canal Univ., Morphometric Analyses. J. Adumbrationibus Illustravit. i-xxxi, ix- Anim. Vet. Adv., 5 (3): 172-175. xvi, 1-243, Plates A-J, L-Q, 1-55. NCMR (2002). Comparative studies of the Lugduni-Batavorum. state of population of Penaeus kerathurus Fabricius, J.C. (1798). Supplementum shrimp in north Mediterranean. In: A.J. entomologiae systematicae. Proft et Storch, CONIDES and F.LUMARE (eds.), Final Hafniae [= Copenhagen], [2] + 572 pp. report, Research Project No. 0037/98 FAO (2011). http://www.fao/org/fishery/ DG-XIV Fisheries: 238. species/fact sheets/Melicertus kerathurus. Nuri BAŞUSTA. (1997). Some Biological Forskål, P. (1775). Descriptions animalium Aspects of Penaeid Shrimp Inhabiting avium, amphibiorum, piscium, insectorum, Yumurtalık Bight in İskenderun Bay vermium; quæ in itinere orientali (North-Eastern Mediterranean) Meting observavit Petrus Forskål. Post mortem KUMLU, Dursun AVŞAR, Tufan auctoris edidit Carsten Niebuhr. EROLDOĞAN Fac. Fisheries, Çukurova Adjuncta est materia medica Kahirina Univ., 01330 Balcalı, Adana-. atque tabula maris rubri geographica. - Otero, M.; Cebrian, E.; Francour, P.; pp. 1-20, [=1-34], 1-164, 1 map. Hauniæ Galil, B. and Savini, D. (2013). (Möller). Monitoring Marine Invasive Species in GAFRD (2009). The general authority for Mediterranean Marine Protected Areas fishery resources development: summary (MPAs): A strategy and practical gui production statistics. Cairo, Egypt. Malaga, Spain: IUCN, 136. Guerra-Garcia, J.M.; Espinosa, F. and Piratheepa, S.; Chitravadivelu, K. and Garcia-Gómez, J.C. (2008). Trends in Edrisinghe, U. (2016). Taxonomic study Taxonomy today: an overview about the and Identification key to the Species of main topics in Taxonomy. Zool. Baetica, the shrimps, particularly the Family 19:15-49. Penaeidae in Kakkaithivu coastal waters, Jaffna, Sri Lanka. Int. J. Sci. and Res. Jayawickrama, S.J.C. (2010). Towards Public., 6 : 5. sustainable utilization of Penaeid prawn resources in Sri Lankan waters. Vidura, Rahnama, R.; Hosseini, S.J.; Qasemi, 13: 17-19. S.A.; Yavari, V.; Zolgharnein, H. and Matinfar, A. (2010). Preiminary Keat-Chuan, N.C.; Aun-Chuan, O.P.; molecular comparison of P. penaeus Wong, W.L. and Khoo, G. (2017). A semisulcatus of Persion Gulf and its Review of Fish Taxonomy Conventions subspecies Penaeus semisulcatus persicus and Species Identification Techniques. J. using 16S rRNA Modern Genetics, 25: Survey in Fisheries Sci., 4 (1): 54-93. 23-31. King, B.H. and King, R.B. (1995). Yassien, M.H. (2004). Biology and fishery Sibmating and its fitness consequences of the green tiger prawn Penaeus in the parasitoid wasp Spalangia semisulcatus De Haan (1850) in Bardawil cameroni (Hymenoptera: Pteromalidae). Lagoon, Northern Sinai, Egypt. Bulletin J. Insect. Behavior, 8: 723-730. of National Inst. Oceanography and Fisheries, ARE, 30:1-22.

Amin, et al. | SINAI Journal of Applied Sciences 10 (1) 2021 039-046 45 الملخص العربً دراسة تصنٍفٍة للتعرف على أنواع الجمبري فً منخفض البردوٌل سمر عبذالرحٍم أمٍن1، جابر دسوقً إبراهٍم2، محمذ سالم أحمذ3 1- طبنت دراسبد ػهٍب، قسى انضزوح انسًكٍخ واألحٍبء انًبئٍخ، كهٍخ انؼهىو انشراػٍخ انجٍئٍخ انؼزٌش، جبيؼخ انؼزٌش، يصز. 2- قسى انضزوح انسًكٍخ واألحٍبء انًبئٍخ، كهٍخ انؼهىو انشراػٍخ انجٍئٍخ انؼزٌش، جبيؼخ انؼزٌش، يصز. 3- كلية االستزراع المائي والمصايد البحرية، جامعة العريش، مصر. رًذ اجزاء دراسخ رصٍُفٍخ نهزؼزف ػهى أَىاع انجًجزي انًىجىدح فً يُخفض انجزدوٌم حٍش رى جًغ ػ 731ٍُخ يٍ انجًجزي يٍ انصٍبدٌٍ وفحصهى واخذ انقٍبسبد انزصٍُفٍخ. اسزُذد انذراسخ انحبنٍخ ثشكم أسبسً ػهى انزًٍش يٍ خالل انًُط انظبهزي ويالحظخ انسًبد انًًٍشح نهؼٍُبد انطبسجخ. ثبسزخذاو يقٍبص رحهٍهً 1.... يجى وكذنك رى اخذ انقٍبسبد انًىرفًٍززٌخ يٍ خالل دراسخ انطىل اإلجًبنً نكم فزد إنى أقزة يى يٍ طزف انًُصخ إنى َهبٌخ انذٌم. رى قٍبص طىل انذرع إنى أقزة يى يٍ طزف انًُصخ إنى انهبيش انخهفً نهذرع. رى رسجٍم انىسٌ اإلجًبنً ألقزة 1.. جى ثبسزخذاو يٍشاٌ انذقخ اإلنكززوًَ. رى دراسخ وفزح األَىاع وانزىسٌغ انًىسًً، صى رى انزقبط ثؼض انصىر ألجشاء يٍ انجًجزي رحذ انًٍكزوسكىة. ورى انزأكٍذ ػهى هىٌخ خًسخ أَىاع يٍ انجًجزي فً انجزدوٌم وجذ اَهى ٌُزًىا إنى صالصخ أجُبص: )Penaeus, Melicertus, Metapenaeus(، وجذ اٌ انجًجزي انسىٌسً يىجىد ثُسجخ 84.54% يٍ إجًبنً انؼٍُبد. وانجًجزي االثٍض ًٌضم 6.1%. وانجًجزي انقشاسي ًٌضم 2.8%. وانجًجزي انًجزقش ًٌضم 3.8% وانجًجزي انًهكً ًٌضم 2.6%. نذنك رىصً انذراسخ ثزكًهخ االثحبس انزصٍُفٍخ ػهى أَىاع انجًجزي انزً رى انزؼزف ػهٍهب ورقذٌى يؼهىيبد وفٍزح ػٍ االَىاع وانُست انًخزهفخ نزىاجذ كم َىع. الكلمات االسترشادٌة: األَىاع، انجًجزي، انزصٍُف، يُخفض انجزدوٌم.

ـــــــــــــــــــــــــــ المحكمـــــــون: 1- أ.د. محمد محمد السيد المر أستاذ بيولوجيا األسماك والمصايد، كلية الزراعة، جامعة قناة السويس، مصر. 2- أ.د. عطيــــه علـــــي عمــر أستاذ بيولوجيا األسماك المساعد، المعهد القومي لعلوم المحيطات ومصايد األسماك، مصر.

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