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R036 Publication Only Basic Science: Biofilm Investigation of Candidaemia Caused by Biofilm-Forming and Non-Biofilm-Forming Isolates: a Multi-Centre Study S

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R036 Publication Only Basic Science: Biofilm Investigation of Candidaemia Caused by Biofilm-Forming and Non-Biofilm-Forming Isolates: a Multi-Centre Study S R036 Publication Only Basic Science: Biofilm Investigation of candidaemia caused by biofilm-forming and non-biofilm-forming isolates: a multi-centre study S. Ozturk1, M. Tasbakan2, D. Metin3, H. Pullukcu2, O. Turhan4, B. Ozhak Baysan5, S. Senol6, C. Cetin6, M. Ertugrul1, *. West Anatolia Fungal Infection Study Group7 1Infectious Diseases and Clinical Microbiology, Adnan Menderes University, Aydin, Turkey ; 2Infectious Diseases and Clinical Microbiology, Ege University, Izmir, Turkey ; 3Medical Microbiology, Ege University, Izmir, Turkey ; 4Infectious Diseases and Clinical Microbiology, Akdeniz University, Antalya, Turkey ; 5Medical Microbiology, Akdeniz University, Antalya, Turkey ; 6Infectious Diseases and Clinical Microbiology, Celal Bayar University, Manisa, Turkey ; 7Infectious Diseases and Clinical Microbiology, and Medical Microbiology, West Anatolia, Turkey Objectives: Bloodstream infections (BSI) due to Candida spp. are common infections amonghospitalized patients and mostly occur as device associated infections causedby biofilm formation medical devices. The aim of this study is to investigatethe ability to produce biofilm of Candidaspp. isolated from blood cultures. Methods: From five university hospitals, 85 patients with Candida BSIs and their first Candida blood isolates were included in the study, in one year period. Patient's data were extracted from hospital records including demographics, comorbidities,invasive procedures, surgery, antibiotic and antifungal usage, hospital length of stay, and death within the 30 days after candidemia. Determination of biofilm formation modified by Toledo-Arana et.all. Organisms were grown for 24 h at 35°C on sabouraud dexrose agar (SDA). A loop of yeast colony were inoculated sabouraud dexrose broth (SDB) supplemented with 8% glucose. Strains were grown overnight in SDB at 35°C, the culture was diluted 1:40 in SDB–8% glucose,and 200 µl of this cell suspension was used to inoculate sterile 96-well polystyrene microtiter plates. The assessment of biofilm formation was performed thrice in three wells for each strain. After 48 h at 35°C, wells were gently washed with 200 µl of phosphate-buffered saline (PBS), dried in an inverted position, and stained with 1% crystal violet for 15 min. The wells were rinsed again, and the crystal violet was solubilized in 200 µl of ethanol-acetone (80:20, vol/vol). The optical dansity at 590 nm was determined using a microplate reader . After removal of cut-off values,OD > 1strains were considered slime positive. , Table :Demographic characteristics of the patients Results: Among 85 isolates 36 (42,4%) was produced biofilm. We did not find any significant relationship between biofilm formation and comorbidities except renal failure., There was no statistically significant correlation between biofilm positivity and invasive procedures, surgery, antibiotic and antifungal usage,hospital length of stay, and death within the 30 days after candidemia mortality likewise. Conclusions: Contrary to expectations, our data show that biofilm growth by Candida was no statistically significant correlation between clinical outcomes. We thought that these results may be related to the small number of strains; so, further studies with more strains are needed to. WEST ANATOLIA FUNGAL INFECTION STUDY GROUP are ; OZTURKS.B.1 ,TASBAKAN M.2 ,METIN D.Y.3 ,PULLUKCU H.2,TURHAN O.4 ,OZHAK BAYSAN B.5 ,SENOL S.6 ,CETINC.B.6 ,ERTUGRUL M.B.1 ,KAYA O.7 ,AVCI M.8,MERMUT G.8 ,SAYIN-KUTLU S.9 ,ERGIN C.10 ,ALP-CAVUS S.11 , AVKAN-OGUZ V.11 ,YAPAR N.11 Infectious Diseases andClinical Microbiology, 1AdnanMenderes University, Aydin, TURKEY; 2Ege University, Izmir, TURKEY; 4AkdenizUniversity, Antalya, TURKEY; 6Celal Bayar University, Manisa, TURKEY;7Suleyman Demirel University, Isparta, TURKEY; 8IzmirEducation and Research Hospital, Izmir, TURKEY; 9PamukkaleUniversity, Denizli, TURKEY; 11Dokuz Eylul University, Izmir, TURKEY Medical Microbiology, 3Ege University,Izmir, TURKEY,5Akdeniz University, Antalya, TURKEY; 10PamukkaleUniversity, Denizli, TURKEY.
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