Product datasheet Anti-AEBP2 Antibody Catalog Number: PB1029

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-AEBP2 Antibody Name AEBP2 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,ICC/IF,FCM Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human AEBP2 recombinant (Position: K424-Q517). Human AEBP2 shares 98.8% amino acid (aa) sequence identity with mouse AEBP2. Purification Immunogen affinity purified. Observed MW 54KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry(Paraffin-embedded Section): 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM): 1-3μg/1x106 cells

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Adipocyte Enhancer-Binding Protein is a protein that in humans is encoded by the evolutionarily well-conserved gene AEBP2. This gene is mapped to 12p12.3. AEBP2 is a DNA-binding transcriptional . It may regulate the migration and development of the cells through the PRC2-mediated epigenetic mechanism and is most likely a targeting protein for the mammalian PRC2 complex.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-AEBP2 Antibody Catalog Number: PB1029

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis of analysis of AEBP2 using AEBP2 using anti- AEBP2 anti-AEBP2 antibody antibody (PB1029).AEBP2 (PB1029). Electrophoresis was detected in paraffin- was performed on a embedded section of 5-20% SDS-PAGE gel at human lung cancer 70V (Stacking gel) / 90V tissues. Heat mediated (Resolving gel) for 2-3 antigen retrieval was hours. The sample well of performed in citrate each lane was loaded buffer (pH6, epitope with 50ug of sample retrieval solution) for 20 under reducing mins. The tissue section conditions. Lane 1: HL-60 was blocked with 10% whole cell lysates.After goat serum. The tissue Electrophoresis, section was then were transferred to a incubated with 1μg/ml Nitrocellulose membrane rabbit anti- AEBP2 at 150mA for 50-90 Antibody (PB1029) minutes. Blocked the overnight at 4°C. membrane with 5% Non- Biotinylated goat anti- fat Milk/ TBS for 1.5 hour rabbit IgG was used as at RT. The membrane was secondary antibody and incubated with rabbit anti- incubated for 30 minutes AEBP2 antigen affinity at 37°C. The tissue purified polyclonal section was developed antibody (Catalog # using Strepavidin-Biotin- PB1029) at 0.5 μg/mL Complex (SABC)(Catalog overnight at 4°C, then # SA1022) with DAB as washed with the chromogen. TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AEBP2 at approximately 54KD. The expected band size for AEBP2 is at 54KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-AEBP2 Antibody Catalog Number: PB1029

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Figure 3. IHC analysis of Figure 4. IHC analysis of AEBP2 using anti- AEBP2 AEBP2 using anti- AEBP2 antibody (PB1029).AEBP2 antibody (PB1029).AEBP2 was detected in paraffin- was detected in paraffin- embedded section of embedded section of human mammary cancer human placenta tissues. tissues. Heat mediated Heat mediated antigen antigen retrieval was retrieval was performed performed in citrate in citrate buffer (pH6, buffer (pH6, epitope epitope retrieval solution) retrieval solution) for 20 for 20 mins. The tissue mins. The tissue section section was blocked with was blocked with 10% 10% goat serum. The goat serum. The tissue tissue section was then section was then incubated with 1μg/ml incubated with 1μg/ml rabbit anti- AEBP2 rabbit anti- AEBP2 Antibody (PB1029) Antibody (PB1029) overnight at 4°C. overnight at 4°C. Biotinylated goat anti- Biotinylated goat anti- rabbit IgG was used as rabbit IgG was used as secondary antibody and secondary antibody and incubated for 30 minutes incubated for 30 minutes at 37°C. The tissue at 37°C. The tissue section was developed section was developed using Strepavidin-Biotin- using Strepavidin-Biotin- Complex (SABC)(Catalog Complex (SABC)(Catalog # SA1022) with DAB as # SA1022) with DAB as the chromogen. the chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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