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Rhodotomla Cresolica Sp. Nov., a Cresol-Assimilating Yeast Species Isolated from Soil

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Rhodotomla Cresolica Sp. Nov., a Cresol-Assimilating Yeast Species Isolated from Soil INTERNATIONAL JOURNALOF SYSTEMATIC BACTERIOLOGY, Apr. 1997, p. 324-327 Vol. 47, No. 2 0020-7713/97/$04,00+0 Copyright 0 1997, International Union of Microbiological Societies Rhodotomla cresolica sp. nov., a Cresol-Assimilating Yeast Species Isolated from Soil WOUTER J. MIDDELHOVEN1* AND FRANS SPAAIJ' La boratorium voor Microbiologie, Wageningen Agricultural University, 6700 EJ Wageningen, The Netherlands, and Labor fur Schimmelpilze und Hefen, 72076 Tubingen, Germany2 A cresol-assimilating yeast strain of a previously undescribed species belonging to the genus Rhodotorula was isolated from soil. The new strain differs from the previously described species of the genus in its pattern of assimilation of carbon and nitrogen compounds, G+C content, and low levels of DNA-DNA-homology. The new species Rhodotorula cresolica is described. The type strain is CBS 7998. Several yeast species are able to assimilate a great variety of Gilford Response UV-VIS spectrophotometer and thermal programming soft- benzene compounds as sole sources of carbon and energy. ware heating at a rate of O.l"C/min. The G+C content was calculated by the following formula: G+C content = (2.08 X T,) - 106.4, where T,,, is the thermal Strains displaying this property have been found in culture denaturation temperature. A standard preparation of Candida parapsilosis CBS collections (5) and in several natural habitats (7, 12 . In a 604 DNA (T,, 70.6"C) was included as a control in every determination. The previous publication, the isolation of yeast strain G36.2 (T = value reported below is the mean 2 standard deviation from three determina- type strain) from soil polluted with ortho-cresol was reported tions. The extent of DNA-DNA reassociation was determined by using the same instrument (3, 13). (7). Inoculation with strain G36T of soil contaminated with Ubiquinone isoprenologs were extracted and purified as described previously o-cresol accelerated the degradation of this pollutant (7), thus (16, 17). To determine the type of coenzyme Q (CoQ), we used reverse-phase demonstrating that strain G36T can successfully compete for thin-layer chromatography, Merck HPTLC RP-18 F254s plates, and acetone- ortho-cresol with the general soil flora. Identification of this acetonitrile (80:20, vol/vol) as the developing solvent (8). The separated com- organism was based on physiological properties (i.e., the pat- ponents were detected under UV light at 254 nm. tern of assimilation of carbon and nitrogen compounds). The results revealed similarity between strain G36T and Rhodoto- RESULTS rula aurantiaca (Saito) Lodder. However, strain G36T lacked Latin diagnosis of Rhodotorula cresolica Middelhoven et the red pigment and the tendency to elongate to cylindrical Spaaij sp. nov. In medio liquid0 dextrosum et peptonum et cells characteristic of R. aurantiaca. Moreover, it grew without extractum levidinis continenti post 3 dies ad 20°C cellulae sunt vitamins. This prompted us to reconsider the taxonomic posi- globosae aut ovoideae (2-11 X 2-9 pm), singulae aut binae. tion of strain G36T. The results of DNA base composition and Post hebdomades 4 sedimentum adest. Pelliculum non for- DNA-DNA reassociation studies led us to propose a novel mantur. Cultura in agar dextrosum et peptonum et extractum species, named Rhodotomla cresolica Middelhoven et Spaaij. levidinis post hebdominades 4, 20"C, viscosa, crema, glabra, Strain G36 (= CBS 7998) is the type strain of this new taxon. nitida, margo integer. In agara farina Zea maydis confect0 post dies 7, 20°C nec mycelium nec pseudomycelium formantur. MATERIALS AND METHODS Fermentatio nulla. Assimilat D-glucosum, D-glucosaminum Isolation of the strain. Acidic sandy soil (pH [H,O] 3.9; moisture content, (lente), D-ribosum (lente), D-xylosum, D-arabinosum (lente), 10%) obtained just beneath the litter layer of a 130-year-old oak grove at sucrosum, maltosum, trehalosum, a-methyl-D-glucosidum, cel- Wageningen, The Netherlands, was supplied with ortho-cresol (about 20 mgL500 lobiosum (lente), arbutinum, melezitosum, glycerolurn, ribi- g of soil). The contaminated soil was incubated at 10°C in a covered beaker for tolum, xylitolum, glucitolum, mannitolum, glucono-&lactonurn, 2 months. From time to time the contents were mixed with a sterile spatula. Yeasts were enriched by shaking a slurry containing 2.5 g of polluted soil with 10 acidum 2-ceto-~-gluconicum, acidum 5-ceto-~-gluconicum, ml of 1% glucose-1% yeast extract for 2 days at 20°C. This culture was used as acidum gluconicum, acidum glucuronicum, acidum galactu- an inoculum for slant cultures (6) containing o-cresol as the sole carbon source. ronicum, acidum lacticum (lente), acidum succinicum, acidum Strain G36T was obtained by streaking material from the zone of growth on YM citricum (lente), ethanolum, propano-1,2-diolum, acidum qui- agar. The strain was maintained in the same medium. Characterization of the strain. Strain G36T was examined for its morpholog- nicum, acidum saccharicum (lente). Non assimilat D-galacto- ical, cultural, and physiological properties by using the standard methods sum, L-sorbosum, L-arabinosum, L-rhamnosum, salicinum, adopted for yeast identification (15). Utilization of carbon and nitrogen sources melibiosum, lactosum, raffinosum, inulinum, amylum solubile, was examined at 25°C on a rotary shaker rotating at a speed of 100 rpm. erythritolum, L-arabinitolum, galactitolum, inositolum, meth- Utilization of nitrogen sources was confirmed by the auxanographic technique. Assimilation of potentially toxic benzene compounds was studied by the slant anolum, butano-2,3-diolum. Assirnilat kalii nitratum, sodii ni- culture method (6). trosum, ethylaminum, L-lysinum, cadaverinum, creatinum, cre- To determine the DNA base composition, cells were grown in 2% GYP (15) atininum. Non assimilat glucosaminum et imidazolum. at 25°C on a rotary shaker until the late log phase. Cells were harvested by Vitamina externa ad crescentiam non necessariae sunt. centrifugation, washed in water and saline EDTA (0.1 M NaCl, 0.15 M EDTA), and broken with a French press. DNA was isolated by the method of Cryer et al. Crescit in 25"C, non crescit in 30°C. Materiae amyloideae non (2) and was purified by centrifugation in a cesium chloride-ethidium bromide formantur. Ureum finditur. Proportio guanini plus cytosini in gradient as described by Maniatis et al. (4). The purity of the DNA preparation acido deoxyribonucleico 49.2 ? 0.4 per centum. was checked by the spectrophotometric method (4). The base composition of the Typus: CBS 7998 isolatus ex terra Wageningen in collectione DNA was determined in 0.1X SSC (1X SSC is 0.15 M sodium chloride plus 0.015 M sodium citrate, pH 7.0) from the thermal denaturation profile (9) with a zymotica Centraalbureau voor Schimmelcultures, Delphi Bata- vorum. Morphological characteristics of the species. After 5 days of * Corresponding author. Mailing address: Laboratorium voor Mi- growth in broth containing glucose (2%, wt/vol), yeast extract crobiologie, Wageningen Agricultural University, P.O. Box 8033, 6700 (0.5%, wt/vol), and peptone (l%,wt/vol) at 20"C, the cells are EJ Wageningen, The Netherlands. globose to ovoid (2 to 11 by 2 to 9 pm). A pellicle is not 324 VOL.47, 1997 RHODOTORULA CRESOLICA SP. NOV. 325 TABLE 1. Assimilation of carbon compounds hydrolyzed. The color reaction with Diazonium Blue B is pos- Assimilation by itive. The G+C content of the nuclear DNA is 49.2 5 0.4 Compound strain G36= mol%. The major CoQ is CoQ 10. Etymology. The specific epithet cresolica pertains to cresols; D-Glucose .................................................................................... +a cresolica, referring to the ability to assimilate cresols. D-Galactose ................................................................................. - L-Sorbose ..................................................................................... - Origin and deposits. Strain G36T was isolated from soil in D-Glucosamine ............................................................................ D Wageningen, The Netherlands. Type strain CBS 7998 has been D-Ribose ...................................................................................... D deposited in the yeast culture collection of the Centraalbureau D-Xylose ....................................................................................... + voor Schimmel-cultures in Delft, The Netherlands, and in the L-Arabinose ................................................................................. - culture collection of the Laboratory of Microbiology in D-Arabinose................................................................................. D Wageningen, The Netherlands. L-Rhamnose................................................................................. - Sucrose ......................................................................................... + Maltose ........................................................................................ + DISCUSSION aa-Trehalose ............................................................................... + Methyl-a-D-glucoside................................................................. + Besides the differences in utilization of carbon and nitrogen Cellobiose .................................................................................... D Salicin ........................................................................................... - compounds (Table 4), our isolate lacks the red pigment, the Arbutin ........................................................................................
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