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Anti-B2 Bradykinin Receptor (B5685)

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Anti-B2 Bradykinin Receptor (B5685) Anti-B2 Bradykinin Receptor produced in rabbit, affinity isolated antibody Catalog Number B5685 Product Description In the central nervous system, kinins are known to 4 Anti-B2 Bradykinin Receptor is produced in rabbit using induce neural tissue damage. The presence of as immunogen a synthetic peptide corresponding to the bradykinin receptors has been noted on the glial C-terminal domain of human B2 Bradykinin Receptor, astrocytes and oligodendrocytes, and recently on conjugated to KLH. The antibody is affinity-purified microglia as well.4 using the immunizing peptide immobilized on agarose. Reagent The antibody specifically recognizes B2 Bradykinin Supplied as a solution of 1 mg/ml in phosphate buffered Receptor in human arterial smooth muscle by saline, pH 7.7, containing 0.1% sodium azide as a immunohistochemistry with formalin-fixed, paraffin- preservative. embedded tissues, and by immunocytochemistry. The immunizing peptide has 83% homology with the rat Precautions and Disclaimer gene and 89% homology with the mouse gene. Other This product is for R&D use only, not for drug, species reactivity has not been confirmed. household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards The nonapeptide Bradykinin (BK) is a member of the and safe handling practices. kinin family, proinflammatory peptides, and is among the most potent and efficacious vasodilator agonists Storage/Stability 1 known. Kinins act on two distinct receptors: B2 and B1 Store at -20 °C in working aliquots. Repeated freezing receptors. There is little B1 Receptor expression in most and thawing, or storage in "frost-free" freezers, is not healthy tissues, but its expression may be induced or recommended. If slight turbidity occurs upon prolonged enhanced by cytokines produced in inflammation. B2 storage, clarify the solution by centrifugation before Receptor is constitutively expressed on various cell use. Working dilution samples should be discarded if types, including endothelial cells, nerve fibers, not used within 12 hours. leukocytes, and mast cells.2 ESTs have been isolated from colon, connective tissue, heart, Product Profile heart/melanocyte/uterus, kidney, lung, placenta, and Immunohistochemistry: a working concentration of thyroid libraries. The effects of the kinins may be direct 2-12 mg/mL is determined using human arterial smooth or associated with the stimulation of secondary muscle mediators of inflammation, including prostanoids, tachykinins, cytokines, mast cell-derived products, and Immunocytochemistry: a working concentration of nitric oxide (NO). 2-12 mg/mL is recommended Bradykinin can exert both negative and protective Note: In order to obtain best results and assay effects. Stimulation of B2 Receptors is implicated in the sensitivities of different techniques and preparations, pathogenesis of inflammation, pain and tissue injury. we recommend determining optimal working dilutions However, bradykinin can also exert cardioprotective by titration test. mechanisms through the vasorelaxant, anti- hypertrophic and anti-atherosclerotic endothelial References mediators NO, prostaglandins and tissue-type 1. Kang, D.S., et al., Mol. Pharmacol., 62. 281-288 3 plasminogen activator. (2002). 2. Souza, D.G., et al., J Immunol., 172, 2542-2548 4. Noda, M., Neurochem. Int., 45, 437-42 (2004). (2004). 3. Heitsch, H., Expert Opin. Investig. Drugs, 12, 759- This product is manufactured by MBL International 770 (2003). Corporation FF,PHC 10/10-1 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip..
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