The Association of Serum Biomarkers with Cardiac Health in Captive Gorillas
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THE ASSOCIATION OF SERUM BIOMARKERS WITH CARDIAC HEALTH IN CAPTIVE GORILLAS A thesis submitted to Kent State University in partial fulfillment of the requirements for the degree of Master of Arts by Eric E. Henthorn August, 2010 Thesis written by Eric E. Henthorn B.S., Kent State University, 2004 M.A., Kent State University, 2010 Approved by: __________________________________________ Dr. Mary Ann Raghanti Advisor __________________________________________ Dr. Richard Meindl Chair, Department of Anthropology __________________________________________ Dr. Timothy Moerland Dean, College of Arts and Sciences ii TABLE OF CONTENTS AKNOWLEDGEMENTS.................................................................................... viii ABSTRACT……………………………………………………………………….1 Chapter I. INTRODUCTION .............................................................................3 Gorilla Status and Conservation .......................................................4 Heart Disease ....................................................................................6 G. g. gorilla Heart Disease ...............................................................6 The Diet/Activity Level Connection .................................................7 A Natural Gorilla Diet ......................................................................8 Captivity and Captive Gorilla Diets ..................................................8 Adipose, Obesity and Thrifty Genotypes........................................10 Metabolic Syndrome .......................................................................13 Glucose and Insulin.........................................................................14 Indices of Insulin Resistance and Sensitivity ..................................15 Leptin ..............................................................................................16 Cholesterol ......................................................................................17 Oxidized LDL .................................................................................18 Prolactin ..........................................................................................19 Ferritin.............................................................................................20 Research Questions and Predictions ...............................................21 II. MATERIALS AND METHODS .....................................................23 Echocardiograph Measures .............................................................23 Enzyme Immunoassays (EIAs) .......................................................24 Insulin Sensitivity ...........................................................................25 Statistical Analyses .........................................................................25 III. RESULTS .......................................................................................27 EIA Validation ................................................................................27 Biomarker and Echocardiograph Values ........................................27 Statistical Analyses .........................................................................30 PCA 1 ..............................................................................................30 iii PCA 2 ..............................................................................................32 PCA 3 ..............................................................................................34 PCA 4 ..............................................................................................36 Correlation Analyses .......................................................................38 Correlation Analysis 1 ....................................................................38 Correlation Analysis 2 ....................................................................46 Correlation Analysis 3 ....................................................................50 Correlation Analysis 4 ....................................................................54 IV. DISCUSSION ................................................................................58 Principal findings ............................................................................58 Implications.....................................................................................59 Great Ape Heart Disease .................................................................64 Confounding Factors .......................................................................66 Assessment ......................................................................................66 Study Limitations ............................................................................67 Future Directions ............................................................................67 V. CONCLUSIONS ..............................................................................69 LITERATURE CITED ..........................................................................................70 APPENDIX A. ......................................................................................................83 APPENDIX B. .....................................................................................................84 iv LIST OF FIGURES 1. PCA 1 scree plot ...............................................................................................31 2. PCA 2 scree plot ...............................................................................................33 3. PCA 3 scree plot ...............................................................................................35 4. PCA 4 scree plot ...............................................................................................37 5. LogEF against age .............................................................................................39 6. LogCholesterol against age ...............................................................................40 7. LogQUICKI against age ...................................................................................40 8. LogLeptin against age .......................................................................................41 9. LogFerritin against age ......................................................................................41 10. LogLeptin and LogCholesterol .......................................................................42 11. LogLeptin and LogQUICKI ...........................................................................42 12. LogLeptin and LogFerritin .............................................................................43 13. LogEF and LogQUICKI controlling for age ..................................................43 14. LogQUICKI and LogLeptin controlling for age .............................................44 15. LogQUICKI and LogFerritin controlling for age ...........................................44 16. LogQUICKI and LogCholesterol controlling for age .....................................45 17. LogProlactin and LogCholesterol controlling for age ....................................45 18. LogCholesterol and LogFerritin controlling for age .......................................46 19. LogIVS and age ..............................................................................................45 v 20. LogIVS and LogFerritin .................................................................................45 21. LogIVS and LogCholesterol ...........................................................................46 22. LogIVS and LogQUICKI controlling for age .................................................46 23. LogIVS and LogProlactin controlling for age ................................................47 24. LogLVPW and age .........................................................................................49 25. LogLVPW and LogFerritin controlling for age ..............................................49 26. LogLVPW and LogProlactin controlling for age ...........................................50 27. LogFerritin and LogoxLDL controlling for age .............................................50 28. LogLVIDd and age .........................................................................................53 29. LogLVIDd and LogProlactin controlling for age ...........................................53 30. LogLVIDd and LogQUICKI controlling for age ............................................54 vi LIST OF TABLES 1. Descriptive statistics of biomarkers for gorillas with heart disease ..................28 2. Descriptive statistics of biomarkers for gorillas without heart disease ............28 3. Descriptive statistics of echocardiograph measures for gorillas with heart disease ...........................................................................29 4. Descriptive statistics of echocardiograph measures for gorillas without heart disease ......................................................................29 5. PCA 1 solution ..................................................................................................31 6. PCA 2 solution ..................................................................................................33 7. PCA 3 solution ..................................................................................................35 8. PCA 4 solution ..................................................................................................37 9. Correlation analysis 1 .......................................................................................39