The Mitochondrial Genome of Oreogeton Sp. (Diptera: Empididae)

Mitochondrial DNA Part B Resources

ISSN: (Print) 2380-2359 (Online) Journal homepage: https://www.tandfonline.com/loi/tmdn20

The mitochondrial genome of Oreogeton sp. (Diptera: Empididae)

Qicheng Yang, Shang Gao, Zhaohui Pan & Ding Yang

To cite this article: Qicheng Yang, Shang Gao, Zhaohui Pan & Ding Yang (2019) The mitochondrial genome of Oreogeton sp. (Diptera: Empididae), Mitochondrial DNA Part B, 4:2, 2365-2366, DOI: 10.1080/23802359.2019.1631129 To link to this article: https://doi.org/10.1080/23802359.2019.1631129

Published online: 12 Jul 2019.

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Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=tmdn20 MITOCHONDRIAL DNA PART B 2019, VOL. 4, NO. 2, 2365–2366 https://doi.org/10.1080/23802359.2019.1631129

MITOGENOME ANNOUNCEMENT The mitochondrial genome of Oreogeton sp. (Diptera: Empididae)

a,b b a b Qicheng Yang , Shang Gao , Zhaohui Pan and Ding Yang aCollege of Food Science, Tibet Agriculture and Animal Husbandry University, Tibet, China; bCollege of Plant Protection, China Agricultural University, Beijing, China

ABSTRACT ARTICLE HISTORY The dance fly Oreogeton sp. belongs to the subfamily Oreogetoninae of Empididae. The mitogenome Received 29 January 2019 of Oreogeton sp. (GenBank accession number: MK639348) was sequenced, the first representative of Accepted 31 May 2019 the mitogenome of the subfamily. The nearly complete mitogenome is 15,718 bp totally, consisting of KEYWORDS 13 protein-coding genes, two rRNAs, and 22 transfer RNAs. All genes have the similar locations and Mitochondrial genome; strands with that of other published species of Empididae. The nucleotide composition biases toward ％ Oreogetoninae; A and T, which together made up 77.2 of the entirety. Bayesian inference analysis strongly supported phylogenetics the monophyly of Empidoidea, Empididae, and Dolichopodidae. This result also suggested that Oreogetoninae was assigned to the sister group to the clade that consists of Trichopezinae and Clinocerinae, and then Empidinae is the sister to the clade that contains Oreogetoninae, Trichopezinae, and Clinocerinae.

Introduction content of PCGs, tRNAs, and rRNAs is 75.0%, 77.3%, and 82.0% respectively. The total length of all 13 PCGs of O. sp. is Empididae is one of the largest families in Diptera with over 11,278 bp. Five PCGs (NAD2, NAD3, ATP8, NAD5 and NAD6) 5000 described species from the world. Both adults and initiated with ATT codons, and six PCGs (COII, COIII, ATP6, larvae of dance fly are predatory, which are the natural NAD4, NAD4L, and CYTB) initiated with ATG codons, CO1 and enemies for the pests that damage crops, fruiters, woods, NAD1 initiated with TCG and ATA as a start codon, respect- and sanitation (Yang et al. 2007). They are widely used as a ively. Twelve PCGs used typical termination codons TAA biological indicator of evaluating the quality of environment except NAD5 used T in Oreogeton sp. and biodiversity (Yang and Yang 2003). Phylogenetic analysis was performed based on the nucleo- The specimens of Oreogeton sp. used for this study were tide sequences of 13 PCGs from 11 Diptera species. Bayesian collected in Diebu County of Gansu by Shuangmei Ding and (BI) analysis generated the phylogenetic tree topologies based identified by Ding Yang. Specimens are deposited in the on the PCGs matrices (Figure 1). According to the phylogen- Entomological Museum of China Agricultural University etic result, the monophyly of Empidoidea was strongly sup- (CAU). The total genomic DNA was extracted from the whole ported. The monophyletic Dolichopodidae, which contains body (except head) of the specimen using the QIAamp DNA Dolichopodinae and Hydrophorinae, was assigned to the sister Blood Mini Kit (Qiagen, Germany) and stored at 20 C until group to the clade of monophyletic Empididae that consists of needed. The mitogenome was amplified and sequenced as Empidinae, Clinocerinae, Oreogetoninae, and Trichopezinae in described in our previous study (Wang et al. 2016). The this study. For the phylogeny within Empididae, Clinocerinae is nearly complete mitogenome of Oreogeton sp. (GenBank the sister to the clade of Trichopezinae, and the accession number: MK639348) is 15,718 bp. It encoded 13 Oreogetoninae was assigned to the sister group to the clade PCGs, 22 tRNA genes, and two rRNA genes and the control that consists of Trichopezinae and Clinocerinae, and then region could not be sequenced entirely in this study and Empidinae is the sister to the clade that contains were similar with related reports before (Li et al. 2017; Zhou Oreogetoninae, Trichopezinae, and Clinocerinae. This result et al. 2017; Gao et al. 2018). All genes have the similar loca- that Dolichopodidae and Empididae are monophyletic respect- tions and strands with that of other published Empididae ively is consistent with the phylogenetic result of the previous species. The nucleotide composition of the mitogenome was research (Yang et al. 2007). The mitogenome of Oreogeton sp. biased toward A and T, with 77.2% of A þ T content could provide the important information for the further stud- (A ¼ 39.8%, T ¼ 37.4%, C ¼ 13.6%, G ¼ 9.3%). The A þ T ies of Empidoidea phylogeny.

CONTACT Zhaohui Pan [email protected] College of Food Science, Tibet Agriculture & Animal Husbandry University, Linzhi, Tibet 860000, China; Ding Yang [email protected] College of Plant Protection, China Agricultural University, Beijing 100193, China These authors contributed equally to this work. ß 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2366 Q. YANG ET AL.

Figure 1. Bayesian phylogenetic tree of 11 Diptera species. The posterior probabilities are labeled at each node. Genbank accession numbers of all sequence used in the phylogenetic tree have been included in the Figure 1 and corresponding to the names of all species.

Disclosure statement Li X, Ding S, Hou P, Liu X, Zhang C, Yang D. 2017. Mitochondrial genome analysis of Ectophasia rotundiventris (Diptera: Tachinidae). Mitochondrial The authors report no conflicts of interest. The authors alone are respon- DNA B. 2:457–458. sible for the content and writing of the paper. Wang K, Li X, Ding S, Wang N, Mao M, Wang M, Yang D. 2016. The complete mitochondrial genome of the Atylotus miser (Diptera: Funding Tabanomorpha: Tabanidae), with mitochondrial genome phylogeny of lower Brachycera (Orthorrhapha). Gene. 586:184–196. This research was supported by the National Natural Science Foundation of Yang D, Yang C. 2003. Fauna Sinica Insecta. Vol. 34. Diptera Empididae. China [No. 31772497] and National Key Research and Development Plan Beijing: Science Press. from the Ministry of Science and Technology of China [2017YFD0201000]. Yang D, Zhang K, Yao G, Zhang J. 2007. World Catalog of References Empididae (Insecta: Diptera). Beijing: China Agricultural University Press. Gao S, Zhou J, Cao Y, Zhang J, Yang D. 2018. The mitochondrial genome Zhou Q, Ding S, Li X, Zhang T, Yang D. 2017. Complete mitochondrial of Trichoclinocera yixianensis (Diptera: Empididae). Mitochondrial DNA B. genome of Allognosta vagans (Diptera, Stratiomyidae). Mitochondrial 3:452–453. DNA B. 2:461–462.