Bull. Natl. Mus. Nat. Sci., Ser. A, 40(1), pp. 15–51, February 22, 2014 Revision of Isoparorchis Southwell, 1913 (Digenea, Hemiuroidea, Isoparorchiidae), Parasites of the Air Bladder of Freshwater Catfishes: a Molecular and Morphological Study Takeshi Shimazu1, Thomas H. Cribb2, Terrence L. Miller3, Misako Urabe4, Nguyen Van Ha5, Tran Thi Binh5 and Marina B. Shed’ko6 1 10486–2 Hotaka-Ariake, Azumino, Nagano 399–8301, Japan E-mail:
[email protected] 2 School of Biological Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia 3 School of Marine and Tropical Biology, James Cook University, Cairns, Queensland 4870, Australia 4 Department of Ecosystem Studies, School of Environmental Sciences, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522–8525, Japan 5 Department of Parasitology, Institute of Ecology and Biological Resources, Vietnamese Academy of Sciences and Technology, 18-Hoang Quoc Viet, Hanoi, Vietnam 6 Institution of Russian Academy of Sciences, Institute of Biological and Soil Sciences, Far Eastern Branch of Russian Academy of Sciences, 159 Stoletiya Street, Vladivostok 690022, Russian Federation (Received 5 November 2013; accepted 18 December 2013) Abstract Isoparorchis Southwell, 1913 (Digenea, Hemiuroidea, Isoparorchiidae), parasites of the air bladder of freshwater catfishes (Osteichthyes, Siluriformes) from East, Southeast and South Asia and Australia, is revised based on a molecular and morphological study. Materials, including syntypes where available, were examined from Russia (Primorskiy Kray), Japan, Vietnam, Cam- bodia, Bangladesh, India and Australia. The entire second internal transcribed spacer region of the ribosomal DNA (ITS2 rDNA) was sequenced for 18 distinct samples. Four of the five previously described species are considered to be valid: Isoparorchis trisimilitubis Southwell, 1913 (type spe- cies) from India, I.