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JOURNAL OF VIROLOGY Volume 68 November 1994 No. 11 MINIREVIEW Molecular Biology of the Human Immunodeficiency Virus Ramu A. Subbramanian and Eric 6831-6835 Accessory Proteins A. Cohen ANIMAL VIRUSES Monoclonal Antibodies against Influenza Virus PB2 and NP J. Baircena, M. Ochoa, S. de la 6900-6909 Polypeptides Interfere with the Initiation Step of Viral Luna, J. A. Melero, A. Nieto, J. mRNA Synthesis In Vitro Ortin, and A. Portela Low-Affinity E2-Binding Site Mediates Downmodulation of Frank Stubenrauch and Herbert 6959-6966 E2 Transactivation of the Human Papillomavirus Type 8 Pfister Late Promoter Template-Dependent, In Vitro Replication of Rotavirus RNA Dayue Chen, Carl Q.-Y. Zeng, 7030-7039 Melissa J. Wentz, Mario Gorziglia, Mary K. Estes, and Robert F. Ramig Improved Self-Inactivating Retroviral Vectors Derived from Paul Olson, Susan Nelson, and 7060-7066 Spleen Necrosis Virus Ralph Dornburg Isolation of a New Foamy Retrovirus from Orangutans Myra 0. McClure, Paul D. 7124-7130 Bieniasz, Thomas F. Schulz, Ian L. Chrystie, Guy Simpson, Adriano Aguzzi, Julian G. Hoad, Andrew Cunningham, James Kirkwood, and Robin A. Weiss Cell Lines Inducibly Expressing the Adeno-Associated Virus Christina Holscher, Markus Horer, 7169-7177 (AAV) rep Gene: Requirements for Productive Replication Jurgen A. Kleinschmidt, of rep-Negative AAV Mutants Hanswalter Zentgraf, Alexander Burkle, and Regine Heilbronn Role of Flanking E Box Motifs in Human Immunodeficiency S.-H. Ignatius Ou, Leon F. 7188-7199 Virus Type 1 TATA Element Function Garcia-Martinez, Eyvind J. Paulssen, and Richard B. Gaynor Characterization and Molecular Basis of Heterogeneity of Fernando Rodriguez, Carlos 7244-7252 the African Swine Fever Virus Envelope Protein p54 Alcaraz, Adolfo Eiras, Rafael J. Yafiez, Javier M. Rodriguez, Covadonga Alonso, Jose F. Rodriguez, and Jose M. Escribano Activation of Adenovirus-Coded Protease and Processing of Ailsa Webster, Ian R. Leith, and 7292-7300 Preterminal Protein Ronald T. Hay A Conserved Helical Element Is Essential for Internal Changyu Wang, Peter Sarnow, and 7301-7307 Initiation of Translation of Hepatitis C Virus RNA Aleem Siddiqui Scanning Mutagenesis of the Arginine-Rich Region of the Michael Hammerschmid, Diana 7329-7335 Human Immunodeficiency Virus Type 1 Rev trans Activator Palmeri, Michael Ruhl, Herbert Jaksche, Irene Weichselbraun, Ernst Bohnlein, Michael H. Malim, and Joachim Hauber The Large Surface Protein of Duck Hepatitis B Virus Is Elizabeth V. L. Grgacic and David 7344-7350 Phosphorylated in the Pre-S Domain A. Anderson Substrate Requirements of Hepatitis C Virus Serine Yasumasa Komoda, Makoto 7351-7357 Proteinase for Intermolecular Polypeptide Cleavage in Hijikata, Shinya Sato, Shin-ichi Escherichia coli Asabe, Koichi Kimura, and Kunitada Shimotohno Continued on following page Continued from preceding page Expression of Semliki Forest Virus nsPl-Specific Pirjo Laakkonen, Marko Hyvonen, 7418-7425 Methyltransferase in Insect Cells and in Escherichia coli Johan Peranen, and Leevi Kaariainen Specific Inhibition of Aphthovirus Infection by RNAs Alfonso Gutierrez, Encarnaci6n 7426-7432 Transcribed from both the 5' and the 3' Noncoding Regions Martinez-Salas, Belen Pintado, and Francisco Sobrino Sequence Requirements for Stable Binding and Function of John A. Chiorini, Stephen M. 7448-7457 Rep68 on the Adeno-Associated Virus Type 2 Inverted Wiener, Roland A. Owens, Sirkka Terminal Repeats. R. M. Kyostio, Robert M. Kotin, and Brian Safer Map Locations of Mouse Hepatitis Virus Kaisong Fu and Ralph S. Baric 7458-7466 Temperature-Sensitive Mutants: Confirmation of Variable Rates of Recombination Specificity of the Hepatitis C Virus NS3 Serine Protease: Alexander A. Kolykhalov, Eugene 7525-7533 Effects of Substitutions at the 3/4A, 4A/4B, 4B/5A, and V. Agapov, and Charles M. Rice 5A/SB Cleavage Sites on Polyprotein Processing Protein-Protein Interactions between Human David J. Spector and Mary J. 7549-7553 Cytomegalovirus IE2-580aa and pUL84 in Lytically Infected Tevethia Cells Intravirion Reverse Transcripts in the Peripheral Blood Hui Zhang, Omar Bagasra, 7591-7597 Plasma of Human Immunodeficiency Virus Type 1-Infected Masahiro Niikura, Bernard J. Individuals Poiesz, and Roger J. Pomerantz A Transcriptionally Controlled trans-Processing Assay: Stephen S. Whitehead and Dennis 7603-7608 Putative Identification of a Vaccinia Virus-Encoded E. Hruby Proteinase Which Cleaves Precursor Protein P25K Expression and Purification of Recombinant Polyomavirus Xiaoyin Cai, Deching Chang, Scott 7609-7613 VP2 Protein and Its Interactions with Polyomavirus Proteins Rottinghaus, and Richard A. Consigli Amino Acid Sequence Analysis of the Proteolytic Cleavage Gregory J. Tobin, Raymond C. 7620-7627 Products of the Bovine Immunodeficiency Virus Gag Sowder II, Daniele Fabris, Marie Precursor Polypeptide Y. Hu, Jane K. Battles, Catherine Fenselau, Louis E. Henderson, and Matthew A. Gonda Sequence Heterogeneity in the Termini of Lymphocytic Barbara J. Meyer and Peter J. 7659-7664 Choriomeningitis Virus Genomic and Antigenomic RNAs Southern Transcription of a Human Neurotropic Virus Promoter in Douglas Kerr, Chun-Fan Chang, 7637-7643 Glial Cells: Effect of YB-1 on Expression of the JC Virus Nancie Chen, Gary Gallia, Ganesh Late Gene Raj, Benjamin Schwartz, and Kamel Khalili VIRUS-CELL INTERACTIONS Mutually Exclusive Interaction of the Adenovirus E4-6/7 Robert J. O'Connor and Patrick 6848-6862 Protein and the Retinoblastoma Gene Product with Internal Hearing Domains of E2F-1 and DP-1 Induction of Herpes Simplex Virus Type 1 Immediate-Early Walter M. Ralph, Jr., Mark S. 6871-6882 Gene Expression by a Cellular Activity Expressed in Vero Cabatingan, and Priscilla A. and NB41A3 Cells after Growth Arrest-Release Schaffer Selective Inhibition of Virus Protein Synthesis by C. Amici, C. Giorgi, A. Rossi, and 6890-6899 Prostaglandin Al: a Translational Block Associated with M. G. Santoro HSP70 Synthesis An Internal Deletion Enhances the Transcriptional Activity Karen L. MacKenzie, Lynn 6924-6932 of a Recombinant Retrovirus in Hematopoietic Cells In Vivo Bonham, and Geoff Symonds Regulation of Susceptibility and Cell Surface Receptor for Oliver T. Keppler, Markus 6933-6939 the B-Lymphotropic Papovavirus by N Glycosylation Herrmann, Monika Oppenlander, Wolfgang Meschede, and Michael Pawlita Continued on following page Continued from preceding page Membrane and Protein Interactions of a Soluble Form of Matthew R. Klimjack, Susan 6940-6946 the Semliki Forest Virus Fusion Protein Jeffrey, and Margaret Kielian Identification and Characterization of an Epstein-Barr Virus Gerhard Laux, Frank Dugrillon, 6947-6958 Nuclear Antigen 2-Responsive cis Element in the Christine Eckert, Brigitte Adam, Bidirectional Promoter Region of Latent Membrane Protein Ursula Zimber-Strobl, and Georg and Terminal Protein 2 Genes W. Bornkamm Interference of Interleukin-10 with Human Neeltje A. Kootstra, Angelique B. 6967-6975 Immunodeficiency Virus Type 1 Replication in Primary van't Wout, Han G. Huisman, Monocyte-Derived Macrophages Frank Miedema, and Hanneke Schuitemaker Mutations in the Cytoplasmic Tail of Herpes Simplex Virus Duncan W. Wilson, Nick 6985-6993 Glycoprotein H Suppress Cell Fusion by a Syncytial Strain Davis-Poynter, and Anthony C. Minson La Autoantigen Alleviates Translational Repression by the 5' Yuri V. Svitkin, Arnim Pause, and 7001-7007 Leader Sequence of the Human Immunodeficiency Virus Nahum Sonenberg Type 1 mRNA Direct Interactions between Autoantigen La and Human Yung-Nien Chang, Daniel J. 7008-7020 Immunodeficiency Virus Leader RNA Kenan, Jack D. Keene, Anne Gatignol, and Kuan-Teh Jeang A Late Adenovirus Factor Induces eIF-4E Yan Zhang, David Feigenblum, 7040-7050 Dephosphorylation and Inhibition of Cell Protein Synthesis and Robert J. Schneider The E2 Transcriptional Repressor Can Compensate for SP1 Caroline Demeret, Moshe Yaniv, 7075-7082 Activation of the Human Papillomavirus Type 18 Early and Frangoise Thierry Promoter PCR-Based Analysis of Herpes Simplex Virus Type 1 Ramesh Ramakrishnan, Myron 7083-7091 Latency in the Rat Trigeminal Ganglion Established with a Levine, and David J. Fink Ribonucleotide Reductase-Deficient Mutant Long-Term Promoter Activity during Herpes Simplex Virus J. R. Lokensgard, D. C. Bloom, 7148-7158 Latency A. T. Dobson, and L. T. Feldman The Transcriptional Regulator YY1 Binds to the Mikio Momoeda, Masako Kawase, 7159-7168 5'-Terminal Region of B19 Parvovirus and Regulates P6 Stephen M. Jane, Koichi Promoter Activity Miyamura, Neal S. Young, and Sachiko Kajigaya A Small Yeast RNA Selectively Inhibits Internal Initiation of Saumitra Das, Peter Coward, and 7200-7211 Translation Programmed by Poliovirus RNA: Specific Asim Dasgupta Interaction with Cellular Proteins That Bind to the Viral 5'-Untranslated Region Host Range and Cell Cycle Activation Properties of Robert Freund, Paul H. Bauer, 7227-7234 Polyomavirus Large T-Antigen Mutants Defective in pRB Harry A. Crissman, E. Morton Binding Bradbury, and Thomas L. Benjamin Interaction of Papillomaviruses with the Cell Surface Richard B. S. Roden, Reinhard 7260-7266 Kirnbauer, A. Bennett Jenson, Douglas R. Lowy, and John T. Schiller Serum Response Factor Has Functional Roles both in Masahiro Fujii, Haruo Tsuchiya, 7275-7283 Indirect Binding to the CArG Box and in the Tatsuya Chuhjo, Tomoyoshi Transcriptional Activation Function of Human T-Cell Minamino, Ken-Ichi Miyamoto, Leukemia Virus Type I Tax and Motoharu Seiki Effect of CD4 Gene Expression on Adenovirus Replication J. Hotta, L. Shi,
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    Genome-wide engineering of an infectious clone of PNAS PLUS herpes simplex virus type 1 using synthetic genomics assembly methods Lauren M. Oldfielda,1, Peter Grzesikb,1, Alexander A. Voorhiesc, Nina Alperovicha, Derek MacMathb, Claudia D. Najeraa, Diya Sabrina Chandrab, Sanjana Prasadb, Vladimir N. Noskova, Michael G. Montaguea,2, Robert M. Friedmand, Prashant J. Desaib,3, and Sanjay Vasheea,3 aDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, Rockville, MD 20850; bDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University, Baltimore, MD 21231; cDepartment of Infectious Diseases, J. Craig Venter Institute, Rockville, MD 20850; and dPolicy Center, J. Craig Venter Institute, La Jolla, CA 92037 Edited by Jef D. Boeke, New York University Langone Medical Center, New York, NY, and approved August 25, 2017 (received for review January 11, 2017) Here, we present a transformational approach to genome engineer- single modification with the BAC-based system takes weeks to ing of herpes simplex virus type 1 (HSV-1), which has a large DNA complete. If multiple changes in the genome are desired, each genome, using synthetic genomics tools. We believe this method will must be made sequentially, greatly increasing the timeframe of enable more rapid and complex modifications of HSV-1 and other making mutant viruses. large DNA viruses than previous technologies, facilitating many The synthetic genomics assembly method described herein has useful applications. Yeast transformation-associated recombination many potential advantages over the BAC-based system (9). It is an was used to clone 11 fragments comprising the HSV-1 strain KOS application of existing tools to engineer large virus genomes and 152 kb genome.
  • Production, Purification and Evaluation of Insect Cell-Expressed Proteins with Diagnostic Potential

    Production, Purification and Evaluation of Insect Cell-Expressed Proteins with Diagnostic Potential

    JYVÄSKYLÄ STUDIES IN BIOLOGICAL AND ENVIRONMENTAL SCIENCE 192 Patrik Michel Production, Purification and Evaluation of Insect Cell-expressed Proteins with Diagnostic Potential JYVÄSKYLÄN YLIOPISTO JYVÄSKYLÄ STUDIES IN BIOLOGICAL AND ENVIRONMENTAL SCIENCE 192 Patrik Michel Production, Purification and Evaluation of Insect Cell-expressed Proteins with Diagnostic Potential Esitetään Jyväskylän yliopiston matemaattis-luonnontieteellisen tiedekunnan suostumuksella julkisesti tarkastettavaksi yliopiston Ambiotica-rakennuksen salissa YAA303 lokakuun 17. päivänä 2008 kello 12. Academic dissertation to be publicly discussed, by permission of the Faculty of Mathematics and Science of the University of Jyväskylä, in the Building Ambiotica, Auditorium YAA303, on October 17, 2008 at 12 o'clock noon. UNIVERSITY OF JYVÄSKYLÄ JYVÄSKYLÄ 2008 Production, Purification and Evaluation of Insect Cell-expressed Proteins with Diagnostic Potential JYVÄSKYLÄ STUDIES IN BIOLOGICAL AND ENVIRONMENTAL SCIENCE 192 Patrik Michel Production, Purification and Evaluation of Insect Cell-expressed Proteins with Diagnostic Potential UNIVERSITY OF JYVÄSKYLÄ JYVÄSKYLÄ 2008 Editors Varpu Marjomäki Department of Biological and Environmental Science, University of Jyväskylä Pekka Olsbo, Marja-Leena Tynkkynen Publishing Unit, University Library of Jyväskylä Jyväskylä Studies in Biological and Environmental Science Editorial Board Jari Haimi, Anssi Lensu, Timo Marjomäki, Varpu Marjomäki Department of Biological and Environmental Science, University of Jyväskylä URN:ISBN:978-951-39-3365-4