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Guide to Microplate Readers a One-Stop Solution for Your Microplate Detection Needs Choose the Microplate Reader That Best Suits Your Research Needs

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Guide to microplate readers A one-stop solution for your microplate detection needs Choose the microplate reader that best suits your research needs ™ Thermo Scientific microplate readers Fluoroskan FL provide flexibility, performance, and Multiskan FC Multiskan SkyHigh Fluoroskan Luminoskan fluorometer and ease of use for a variety of microplate Considerations photometer spectrophotometer fluorometer luminometer luminometer Varioskan LUX scanning multimode reader Absorbance, fluorescence assays. Whether you need to Fluorescence, Applications Absorbance Fluorescence Luminescence Optional: time-resolved fluorescence (TRF), luminescence measure fluorescence, absorbance, luminescence, AlphaScreen readout luminescence, or time-resolved Absorbance and fluorescence excitation: 200–1,000 nm fluorescence, or perform AlphaScreen™ Fluorescence emission: 370–840 nm assays, we offer a microplate reader Fluorescence: Luminescence: 360–670 nm solution to meet the needs of your excitation: 320– specific workflow. With a portfolio of Excitation: 320– 700 nm; TRF excitation: fixed to 334 nm Wavelength range 340–850 nm 200–1,000 nm 700 nm; emission: 270–670 nm emission: 360–670 nm (spectral scanning 200–840 nm) dedicated and modular, upgradeable 360–800 nm TRF emission: 400–700 nm Luminescence: multimode readers, we also offer (spectral scanning 270–840 nm) solutions that fit your current budget 270–670 nm AlphaScreen excitation: fixed to 680 nm with options to meet your lab’s AlphaScreen emission: 400–660 nm future needs. Monochromator for UV-Vis absorbance Not required for most and fluorescence intensity Thermo Scientific plate readers Wavelength selection Filters Monochromator Filters applications Filters Filters can be used when necessary Filters for luminescence when necessary, TRF, have a number of features to AlphaScreen assays help you save time and maximize ™ ™ Thermo Scientific µDrop 6–1,536 wells productivity, including: 96 wells and µDrop™ Duo Plates, Plate format 6–384 wells 6–384 wells 6–384 wells (fluorometry, TRF, luminometry, AlphaScreen assays) (384 wells optional) 6-48 well plates*, 96 and • Auto-calibration 384 well plates µDrop and µDrop Duo Plates/6–384 wells (absorbance) Incubation Optional Yes Yes Yes Yes Yes • Easy export to Microsoft™ Excel™ format Shaking Yes Yes Yes Yes Yes Yes Dispensers No No Optional (to three)** Optional (to three)** Optional (to three)** Optional (to two) • Automation readiness with Top (standard) robot compatibility Top/bottom reading NA NA Top/bottom† Top Top/bottom† Bottom (optional)† • No limit to the number of computers Cuvettes No Optional No No No With μDrop Plate on which you can install the intuitive Gas control module No No No No No Optional Thermo Scientific™ SkanIt™ Software • Ready-to-use protocols available in an extensive online protocol library For more information, go to thermofisher.com/platereaders * Maximum plate height with lid 19.5 mm. ** Third dispenser requires additional installation. † Instruments with bottom-read capabilities feature multilocation reads per well. 2 Fluoroskan FL Multiskan FC Multiskan SkyHigh Fluoroskan Luminoskan fluorometer and Considerations photometer spectrophotometer fluorometer luminometer luminometer Varioskan LUX scanning multimode reader Absorbance, fluorescence Fluorescence, Applications Absorbance Fluorescence Luminescence Optional: time-resolved fluorescence (TRF), luminescence luminescence, AlphaScreen readout Absorbance and fluorescence excitation: 200–1,000 nm Fluorescence emission: 370–840 nm Fluorescence: Luminescence: 360–670 nm excitation: 320– Excitation: 320– 700 nm; TRF excitation: fixed to 334 nm Wavelength range 340–850 nm 200–1,000 nm 700 nm; emission: 270–670 nm emission: 360–670 nm (spectral scanning 200–840 nm) 360–800 nm TRF emission: 400–700 nm Luminescence: (spectral scanning 270–840 nm) 270–670 nm AlphaScreen excitation: fixed to 680 nm AlphaScreen emission: 400–660 nm Monochromator for UV-Vis absorbance Not required for most and fluorescence intensity Wavelength selection Filters Monochromator Filters applications Filters Filters can be used when necessary Filters for luminescence when necessary, TRF, AlphaScreen assays ™ ™ Thermo Scientific µDrop 6–1,536 wells 96 wells and µDrop™ Duo Plates, Plate format 6–384 wells 6–384 wells 6–384 wells (fluorometry, TRF, luminometry, AlphaScreen assays) (384 wells optional) 6-48 well plates*, 96 and 384 well plates µDrop and µDrop Duo Plates/6–384 wells (absorbance) Incubation Optional Yes Yes Yes Yes Yes Shaking Yes Yes Yes Yes Yes Yes Dispensers No No Optional (to three)** Optional (to three)** Optional (to three)** Optional (to two) Top (standard) Top/bottom reading NA NA Top/bottom† Top Top/bottom† Bottom (optional)† Cuvettes No Optional No No No With μDrop Plate Gas control module No No No No No Optional * Maximum plate height with lid 19.5 mm. ** Third dispenser requires additional installation. † Instruments with bottom-read capabilities feature multilocation reads per well. 3 A range of microplate readers for maximum flexibility and performance To measure absorbance • Fast operation: full spectrum (200–1,000 nm) of a well Thermo Scientific™ Multiskan™ FC is obtained in less than 10 seconds and a full 96-well Microplate Photometer microplate is read in 6 seconds A robust and reliable filter-based • Access to Connect or Microsoft™ OneDrive™ cloud-based instrument that can be used for a tools allows you to securely store, access, share, and wide variety of research and routine manage data remotely (touchscreen models) applications. It can be used as a stand-alone instrument or under PC control with intuitive thermofisher.com/multiskanskyhigh SkanIt Software. To measure fluorescence • Wide variety of applications, including ELISAs, endotoxin Thermo Scientific™ Fluoroskan™ assays, cytotoxicity assays, and growth curves Microplate Fluorometer • Shaking and incubation up to 50°C for Featuring excellent optical performance, temperature-sensitive assays the Fluoroskan Microplate Fluorometer is ideal for life science research applications • Proven performance and reliable day-to-day results such as fluorometric protein and enzyme studies, molecular through patented optical design and self-diagnostics interaction studies, and nucleic acid quantification, as well thermofisher.com/multiskanfc as reporter gene, fluorometric kinase, immunological, and cell-based assays. Thermo Scientific™ Multiskan™ • High sensitivity for both top and bottom reading SkyHigh Microplate • Onboard dispensers for precise initiation of Spectrophotometer kinetic reactions The Multiskan SkyHigh Microplate Spectrophotometer is easy to use • Can be equipped with up to three reagent dispensers for for any photometric or turbidimetric use during fast kinetic assays, such as Ca2+ flux assays research application, especially nucleic acid and protein thermofisher.com/fluoroskan analysis. It offers an optional easy-to-use graphic touchscreen user interface and multiple connectivity To measure luminescence options (USB, computer, or cloud). Thermo Scientific™ Luminoskan™ • Allows kinetic, spectral, and endpoint measurements for Microplate Luminometer a variety of applications Useful for a variety of luminometric research applications, the Luminoskan • Separates optimized measurement modes for absorption Microplate Luminometer is a compact and turbidimetric measurements and robust instrument offering plate format versatility, • Fast reading speed essential for kinetic applications fast reading speeds, and up to three dispensers. The Luminoskan luminometer provides simplicity and • Available in three different configurations: 1) touchscreen, reliability for increased efficiency of workflows. 2) cuvette and touchscreen, and 3) operated only with SkanIt PC Software • Fast reading speed essential for kinetic applications • Reads µDrop and µDrop Duo plates for microvolume • Onboard dispensers for precise initiation of analysis of DNA, RNA, and protein kinetic reactions • Models with a touchscreen have an easy-to-use interface • Capable of reading a 96-well plate in just 18 seconds, for stand-alone use, including ready-made protocols for essential for kinetic applications such as enzyme kinetic UV-based nucleic acid and protein quantification as well studies and phagocytosis assays as colorimetric protein quantification thermofisher.com/luminoskan 4 To measure fluorescence and luminescence Software for readout Thermo Scientific™ Fluoroskan™ FL Microplate SkanIt Software Fluorometer and Luminometer The intuitive interface of our updated SkanIt Software Featuring excellent optical performance, will guide you through the measurement process to help the Fluoroskan FL Microplate you get the results you need. With SkanIt Software, you Fluorometer and Luminometer is ideal have full control over the instrument settings for all your for fluorometric and luminometric Thermo Scientific microplate readers. SkanIt Software assays in life science research. The is available in two editions. The research edition is for Fluoroskan FL fluorometer and luminometer offers plate scientists working in life science research, and the drug format versatility, fast plate reading speeds, and up to discovery edition provides features to help you comply with three dispensers. the requirements of FDA 21 CFR Part 11. • High sensitivity for both top and bottom reading SkanIt Software makes microplate reading easy • Fast reading speed essential for kinetic applications SkanIt Software provides excellent usability and flexibility, even for the most challenging
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