Laboratory Procedure Manual Analyte: Trihalomethanes/MTBE/Nitromethane Matrix: Whole Blood Method: Solid Phase Microextraction with GC Separation/High Resolution MS Method No: 2101.01 Revised: April 30, 2015 As performed by: Tobacco & Volatiles Branch Division of Laboratory Sciences National Center for Environmental Health Contact: Dr. Ben Blount Phone: 770-488-7894 Fax: 770-488-0181 Email:
[email protected] James L. Pirkle, M.D., Ph.D. Director, Division of Laboratory Sciences Important Information for Users The Centers for Disease Control and Prevention (CDC) periodically refines these laboratory methods. It is the responsibility of the user to contact the person listed on the title page of each write-up before using the analytical method to find out whether any changes have been made and what revisions, if any, have been incorporated. THMs & MTBE VOCs in Blood DLS Method Code: 2101.01 National Center for Health Staistics 2 This document details the Lab Protocol for testing the items listed in the following table Data File Name Variable Name SAS Label LBXVBF Blood Bromoform (pg/mL) LBXVBM Blood Bromodichloromethane (pg/mL) VOCMWB_F LBXVCF Blood Chloroform (pg/mL) LBXVCM Blood Dibromochloromethane (pg/mL) LBXVME Blood MTBE (pg/mL) LBXVNM Blood Nitromethane (pg/mL) THMs & MTBE VOCs in Blood DLS Method Code: 2101.01 National Center for Health Staistics 3 1. Clinical Relevance and Summary of Test Principle a. Clinical Relevance The prevalence of disinfection by-products in drinking water supplies has raised concerns about possible adverse health effects from chronic exposure to these potentially carcinogenic compounds. To support studies exploring the relation between exposure to trihalomethanes (THMs), nitromethane (NM: biomarker for halonitromethanes), methyl tert-butyl ether (MTBE) and adverse health effects, an automated analytical method was developed using capillary gas chromatography (GC) and high-resolution mass spectrometry (MS) with selected ion mass detection and isotope-dilution techniques.