DOCSLIB.ORG

Kroppenstedtia Pulmonis Sp. Nov. and Kroppenstedtia Sanguinis Sp. Nov., Isolated from Human Patients

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Kroppenstedtia Pulmonis Sp. Nov. and Kroppenstedtia Sanguinis Sp. Nov., Isolated from Human Patients Antonie van Leeuwenhoek (2016) 109:603–610 DOI 10.1007/s10482-016-0663-z ORIGINAL PAPER Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients Melissa E. Bell . Brent A. Lasker . Hans-Peter Klenk . Lesley Hoyles . Catherine Spro¨er . Peter Schumann . June M. Brown Received: 30 September 2015 / Accepted: 2 February 2016 / Published online: 24 February 2016 Ó Springer International Publishing Switzerland (outside the USA) 2016 Abstract Three human clinical strains (W9323T, relatedness was 94.6 %, confirming that X0209T and X0209T and X0394) isolated from a lung biopsy, blood X0394 belong to the same species. Chemotaxonomic and cerebral spinal fluid, respectively, were charac- data for strains W9323T and X0209T were consistent terised using a polyphasic taxonomic approach. Com- with those described for the members of the genus parative analysis of the 16S rRNA gene sequences Kroppenstedtia: the peptidoglycan was found to con- showed the three strains belong to two novel branches tain LL-diaminopimelic acid; the major cellular fatty within the genus Kroppenstedtia: 16S rRNA gene acids were identified as iso-C15 and anteiso-C15; and sequence analysis of W9323T showed close sequence the major menaquinone was identified as MK-7. similarity to Kroppenstedtia eburnea JFMB-ATET Differences in endospore morphology, carbon source (95.3 %), Kroppenstedtia guangzhouensis GD02T utilisation profiles, and cell wall sugar patterns of (94.7 %) and strain X0209T (94.6 %); sequence anal- strains W9323T and X0209T, supported by phyloge- ysis of strain X0209T showed close sequence similarity netic analysis, enabled us to conclude that the strains to K. eburnea JFMB-ATET (96.4 %) and K. each represent a new species within the genus Krop- guangzhouensis GD02T (96.0 %). Strains X0209T penstedtia, for which the names Kroppenstedtia pul- and X0394 were 99.9 % similar to each other by 16S monis sp. nov. (type strain W9323T = DSM rRNA gene sequence analysis. The DNA–DNA 45752T = CCUG 68107T) and Kroppenstedtia & M. E. Bell ( ) C. Spro¨er Á P. Schumann Centers for Disease Control and Prevention, Building 17, Leibniz-Institute DSMZ-German Collection of Room 2209, Mailstop D-11, 1600 Clifton Road, Atlanta, Microorganisms and Cell Cultures, 38124 Brunswick, GA 30333, USA Germany e-mail: [email protected] L. Hoyles B. A. Lasker Á J. M. Brown Department of Biomedical Sciences, Faculty of Science & Bacterial Special Pathogens Branch, Division of High- Technology, University of Westminster, 115 New Consequence Pathogens and Pathology, National Center Cavendish Street, London W1W 6UW, UK for Emerging and Zoonotic Infectious Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA H.-P. Klenk Head of School, School of Biology, Newcastle University, Ridley Building 2, Newcastle upon Tyne NE1 7RU, UK 123 604 Antonie van Leeuwenhoek (2016) 109:603–610 sanguinis sp. nov. (type strain X0209T = DSM Special Bacteriology Reference Laboratory (SBRL), 45749T = CCUG 38657T) are proposed. Atlanta, GA. Genotypic and phenotypic data suggest that strain W9323T represents a new species, for which Keywords Kroppenstedtia species Á Kroppenstedtia we propose the name Kroppenstedtia pulmonis sp. pulmonis Á Kroppenstedtia sanguinis Á Polyphasic nov., and that strains X0209T and X0394 both taxonomy Á 16S rRNA gene Á Thermoactinomycetes represent another new species, for which we propose the name Kroppenstedtia sanguinis sp. nov. Introduction Materials and methods The family Thermoactinomycetaceae was established to Bacterial strains accommodate new taxa (Mechercharimyces spp.) and the previously described genera Thermoactinomyces, Strain X0209T was obtained from the Culture Collec- Laceyella, Thermoflavimicrobium, Seinonella and tion University of Go¨teborg (CCUG), Go¨teborg, Swe- Planifilum (Matsuo et al. 2006). The family now den as ‘Thermoactinomyces sanguinis’(CCUG encompasses 19 genera, with representatives isolated 38657T). Two strains, W9323T and X0394, were sent from clinical specimens (e.g. Desmospora, Marinither- to the SBRL, Centers for Disease Control and Preven- mofilum, Hazenella) or environmental sources (e.g. tion for identification. K. eburnea DSM 45196T was Mechercharimyces, Lihuaxuella, Geothermomicrobium used as a phenotypic, chemotaxonomic and genetic and Risungbinella) (Matsuo et al. 2006; Yassin et al. control and K. guangzhouensis GD02T and Melghir- 2009;Yuetal.2012;Bussetal.2013;Zhouetal.2014; imyces algeriensis NariEXT were used as chemotaxo- Kim et al. 2015; Zhang et al. 2015). Salient properties of nomic and genetic controls throughout this study. The the family Thermoactinomycetaceae include thermo- GenBank accession numbers for the 16S rRNA gene tolerant growth up to 60 °C, Gram positivity, non-acid sequences and the patients’ data associated with these fastness and formation of single spores that may be strains are given in Table 1. sessile or on simple sporophores with the structure and properties of endospores. The description of the family Phenotypic analyses was emended by establishing the 16S rRNA gene sequence signature nucleotides appropriate for supra- Morphological, cultural, physiological generic assignment (Yassin et al. 2009). Von Jan et al. and biochemical analyses (2011) further emended the description of the family Thermoactinomycetaceae (to contain either LL-di- To examine morphologic features, strains were grown aminopimelic acid or meso-diaminopimelic acid) when aerobically using brain heart infusion (BHI) broth, they described a new genus and species, Kroppenstedtia heart infusion agar (HIA; Becton, Dickinson and Co, eburnea, that contains the isomer LL-diaminopimelic Sparks, MD) supplemented with 5 % rabbit blood, acid in its peptidoglycan. HIA slants, trypticase soy agar (TSA) supplemented Kroppenstedtia species have been isolated from with 5 % sheep blood (Becton, Dickinson and Co) at environmental and clinical sources. Although the type 35 and 45 °C for 3 to 7 days and then examined for strain of K. eburnea was isolated from an environ- microscopic and macroscopic features. Gram and mental source (a plastic surface in a contract manu- modified Kinyoun acid-fast staining were conducted facturing company in Germany), Barker et al. (2012) as described previously (Berd 1973). Cultures were identified 14 strains of this species in clinical samples examined for optimal growth at 35, 45, 50 and 60 °C (blood, skin, peritoneal fluid, cerebral spinal fluid) in for 7 days on HIA slants with 5 % rabbit blood. the United States. Another species, Kroppenstedtia Optimal growth was determined by comparative guangzhouensis, was isolated from soil in south China observation of the amount of cell mass produced at (Yang et al. 2013). Three human clinical strains of each temperature. All phenotypic studies were per- Kroppenstedtia species were identified in a retrospec- formed under optimal growth conditions (at 45 °Cin tive evaluation of 16S rRNA gene sequences at the air). 123 Antonie van Leeuwenhoek (2016) 109:603–610 605 Table 1 Strains used in this study Strain Date received or Source Geographic GenBank accession reference source number of 16S rRNA gene Kroppenstedtia eburnea (DSM 45196T) von Jan et al. (2011) Plastic surface Germany FN665656 K. guangzhouensis (GD02T) Yang et al. (2013) Soil China KC311557 Melghirimyces algeriensis (NariEXT) Addou et al. (2012) Soil from salt lake Algeria HQ383683 W9323T 2008 Lung, 78-year-old male New York, USA KC875234 X0209T 1997 Blood, 59-year-old male Ga¨vle, Sweden AJ251778 X0394 2010 CSF, 16-year-old female Quebec, Canada KC875233 Decomposition tests for adenine, casein, esculin, analysed by the method of Minnikin et al. (1984). hypoxanthine, tyrosine, urea and xanthine, utilisation Analysis of isoprenoid quinones by HPLC was of 22 carbohydrates as sole source of carbon, utilisa- performed as described by Kroppenstedt (1982, tion of acetamide and citrate, arylsulfatase production 1985). Cellular fatty acids were prepared by the and nitrate reduction and growth in the presence of method of Klatte et al. (1994) and the fatty acid methyl lysozyme were performed as described previously esters were then separated as described by Sasser (Conville and Witebsky 2007; Conville et al. 2008; (1990) using the Microbial Identification System Weyant et al. 1996; Yassin et al. 1995). The decom- (MIDI, Inc., Sherlock version 6.1). Standardisation position of casein was compared with casein plus of the physiological age of the study and reference 0.5 % NaCl as described by von Jan et al. (2011). strains was obtained by choosing the sector from a quadrant streak of culture plates. Antimicrobial susceptibility testing Genetic analyses Since no guidelines were available for the genus Kroppenstedtia, the MICs to 10 antimicrobial agents 16S rRNA gene sequence analysis were determined following interpretative breakpoints as recommended for aerobic actinomycetes (Clinical Purification of whole-cell DNA, amplification of near and Laboratory Standards Institute 2011) for amikacin, full-length 16S rRNA gene fragments, primers and amoxicillin-clavulanate, ceftriaxone, ciprofloxacin, nucleotides for PCR, purification of amplicons and clarithromycin, imipenem, linezolid, minocycline, DNA cycle sequencing were described previously moxifloxacin and trimethoprim-sulfamethoxazole; the (Lasker et al. 2011). Consensus 16S rRNA gene interpretive breakpoint for ampicillin used was that sequences were assembled and edited using recommended for Enterobacteriaceae (Clinical and Sequencher version 4.10.1
Load more

Recommended publications
  • Archaea, Bacteria and Termite, Nitrogen Fixation and Sustainable Plants Production
    Sun W et al . (2021) Notulae Botanicae Horti Agrobotanici Cluj-Napoca Volume 49, Issue 2, Article number 12172 Notulae Botanicae Horti AcademicPres DOI:10.15835/nbha49212172 Agrobotanici Cluj-Napoca Re view Article Archaea, bacteria and termite, nitrogen fixation and sustainable plants production Wenli SUN 1a , Mohamad H. SHAHRAJABIAN 1a , Qi CHENG 1,2 * 1Chinese Academy of Agricultural Sciences, Biotechnology Research Institute, Beijing 100081, China; [email protected] ; [email protected] 2Hebei Agricultural University, College of Life Sciences, Baoding, Hebei, 071000, China; Global Alliance of HeBAU-CLS&HeQiS for BioAl-Manufacturing, Baoding, Hebei 071000, China; [email protected] (*corresponding author) a,b These authors contributed equally to the work Abstract Certain bacteria and archaea are responsible for biological nitrogen fixation. Metabolic pathways usually are common between archaea and bacteria. Diazotrophs are categorized into two main groups namely: root- nodule bacteria and plant growth-promoting rhizobacteria. Diazotrophs include free living bacteria, such as Azospirillum , Cupriavidus , and some sulfate reducing bacteria, and symbiotic diazotrophs such Rhizobium and Frankia . Three types of nitrogenase are iron and molybdenum (Fe/Mo), iron and vanadium (Fe/V) or iron only (Fe). The Mo-nitrogenase have a higher specific activity which is expressed better when Molybdenum is available. The best hosts for Rhizobium legumiosarum are Pisum , Vicia , Lathyrus and Lens ; Trifolium for Rhizobium trifolii ; Phaseolus vulgaris , Prunus angustifolia for Rhizobium phaseoli ; Medicago, Melilotus and Trigonella for Rhizobium meliloti ; Lupinus and Ornithopus for Lupini, and Glycine max for Rhizobium japonicum . Termites have significant key role in soil ecology, transporting and mixing soil. Termite gut microbes supply the enzymes required to degrade plant polymers, synthesize amino acids, recycle nitrogenous waste and fix atmospheric nitrogen.
    [Show full text]
  • Extremozymes of the Hot and Salty Halothermothrix Orenii
    Extremozymes of the Hot and Salty Halothermothrix orenii Author Kori, Lokesh D Published 2012 Thesis Type Thesis (PhD Doctorate) School School of Biomolecular and Physical Sciences DOI https://doi.org/10.25904/1912/2191 Copyright Statement The author owns the copyright in this thesis, unless stated otherwise. Downloaded from http://hdl.handle.net/10072/366220 Griffith Research Online https://research-repository.griffith.edu.au Extremozymes of the hot and salty Halothermothrix orenii LOKESH D. KORI (M.Sc. Biotechnology) School of Biomolecular and Physical Sciences Science, Environment, Engineering and Technology Griffith University, Australia Submitted in fulfillment of the requirements of the degree of Doctor of Philosophy December 2011 STATEMENT OF ORIGINALITY STATEMENT OF ORIGINALITY This work has not previously been submitted for a degree or diploma in any university. To the best of my knowledge and belief, the thesis contains no material previously published or written by another person except where due reference is made in the thesis itself. LOKESH DULICHAND KORI II ACKNOWLEDGEMENTS ACKNOWLEDGEMENTS I owe my deepest gratitude to my supervisor Prof. Bharat Patel, for offering me an opportunity for being his postgraduate. His boundless knowledge motivates me for keep going and enjoy the essence of science. Without his guidance, great patience and advice, I could not finish my PhD program successfully. I take this opportunity to give my heartiest thanks to Assoc. Prof. Andreas Hofmann, (Structural Chemistry, Eskitis Institute for Cell & Molecular Therapies, Griffith University) for his support and encouragement for crystallographic work. I am grateful to him for teaching me about the protein structures, in silico analysis and their hidden chemistry.
    [Show full text]
  • Desulfuribacillus Alkaliarsenatis Gen. Nov. Sp. Nov., a Deep-Lineage
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central Extremophiles (2012) 16:597–605 DOI 10.1007/s00792-012-0459-7 ORIGINAL PAPER Desulfuribacillus alkaliarsenatis gen. nov. sp. nov., a deep-lineage, obligately anaerobic, dissimilatory sulfur and arsenate-reducing, haloalkaliphilic representative of the order Bacillales from soda lakes D. Y. Sorokin • T. P. Tourova • M. V. Sukhacheva • G. Muyzer Received: 10 February 2012 / Accepted: 3 May 2012 / Published online: 24 May 2012 Ó The Author(s) 2012. This article is published with open access at Springerlink.com Abstract An anaerobic enrichment culture inoculated possible within a pH range from 9 to 10.5 (optimum at pH with a sample of sediments from soda lakes of the Kulunda 10) and a salt concentration at pH 10 from 0.2 to 2 M total Steppe with elemental sulfur as electron acceptor and for- Na? (optimum at 0.6 M). According to the phylogenetic mate as electron donor at pH 10 and moderate salinity analysis, strain AHT28 represents a deep independent inoculated with sediments from soda lakes in Kulunda lineage within the order Bacillales with a maximum of Steppe (Altai, Russia) resulted in the domination of a 90 % 16S rRNA gene similarity to its closest cultured Gram-positive, spore-forming bacterium strain AHT28. representatives. On the basis of its distinct phenotype and The isolate is an obligate anaerobe capable of respiratory phylogeny, the novel haloalkaliphilic anaerobe is suggested growth using elemental sulfur, thiosulfate (incomplete as a new genus and species, Desulfuribacillus alkaliar- T T reduction) and arsenate as electron acceptor with H2, for- senatis (type strain AHT28 = DSM24608 = UNIQEM mate, pyruvate and lactate as electron donor.
    [Show full text]
  • Developing a Genetic Manipulation System for the Antarctic Archaeon, Halorubrum Lacusprofundi: Investigating Acetamidase Gene Function
    www.nature.com/scientificreports OPEN Developing a genetic manipulation system for the Antarctic archaeon, Halorubrum lacusprofundi: Received: 27 May 2016 Accepted: 16 September 2016 investigating acetamidase gene Published: 06 October 2016 function Y. Liao1, T. J. Williams1, J. C. Walsh2,3, M. Ji1, A. Poljak4, P. M. G. Curmi2, I. G. Duggin3 & R. Cavicchioli1 No systems have been reported for genetic manipulation of cold-adapted Archaea. Halorubrum lacusprofundi is an important member of Deep Lake, Antarctica (~10% of the population), and is amendable to laboratory cultivation. Here we report the development of a shuttle-vector and targeted gene-knockout system for this species. To investigate the function of acetamidase/formamidase genes, a class of genes not experimentally studied in Archaea, the acetamidase gene, amd3, was disrupted. The wild-type grew on acetamide as a sole source of carbon and nitrogen, but the mutant did not. Acetamidase/formamidase genes were found to form three distinct clades within a broad distribution of Archaea and Bacteria. Genes were present within lineages characterized by aerobic growth in low nutrient environments (e.g. haloarchaea, Starkeya) but absent from lineages containing anaerobes or facultative anaerobes (e.g. methanogens, Epsilonproteobacteria) or parasites of animals and plants (e.g. Chlamydiae). While acetamide is not a well characterized natural substrate, the build-up of plastic pollutants in the environment provides a potential source of introduced acetamide. In view of the extent and pattern of distribution of acetamidase/formamidase sequences within Archaea and Bacteria, we speculate that acetamide from plastics may promote the selection of amd/fmd genes in an increasing number of environmental microorganisms.
    [Show full text]
  • Antonie Van Leeuwenhoek Journal of Microbiology
    Antonie van Leeuwenhoek Journal of Microbiology Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients --Manuscript Draft-- Manuscript Number: ANTO-D-15-00548R1 Full Title: Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients Article Type: Original Article Keywords: Kroppenstedtia species, Kroppenstedtia pulmonis, Kroppenstedtia sanguinis, polyphasic taxonomy, 16S rRNA gene, thermoactinomycetes Corresponding Author: Melissa E Bell, MS Centers for Disease Control and Prevention Atlanta, Georgia UNITED STATES Corresponding Author Secondary Information: Corresponding Author's Institution: Centers for Disease Control and Prevention Corresponding Author's Secondary Institution: First Author: Melissa E Bell, MS First Author Secondary Information: Order of Authors: Melissa E Bell, MS Brent A. Lasker, PhD Hans-Peter Klenk, PhD Lesley Hoyles, PhD Catherine Spröer Peter Schumann June Brown Order of Authors Secondary Information: Funding Information: Abstract: Three human clinical strains (W9323T, X0209T and X0394) isolated from lung biopsy, blood and cerebral spinal fluid, respectively, were characterized using a polyphasic taxonomic approach. Comparative analysis of the 16S rRNA gene sequences showed the three strains belonged to two novel branches within the genus Kroppenstedtia: 16S rRNA gene sequence analysis of W9323T showed closest sequence similarity to Kroppenstedtia eburnea JFMB-ATE T (95.3 %), Kroppenstedtia guangzhouensis GD02T (94.7 %) and strain X0209T (94.6 %); sequence analysis of strain X0209T showed closest sequence similarity to K. eburnea JFMB-ATE T (96.4 %) and K. guangzhouensis GD02T (96.0 %). Strains X0209T and X0394 were 99.9 % similar to each other by 16S rRNA gene sequence analysis. The DNA-DNA relatedness was 94.6 %, confirming that X0209T and X0394 belong to the same species.
    [Show full text]
  • Urukthapelstatin A, a Novel Cytotoxic Substance from Marine-Derived Mechercharimyces Asporophorigenens YM11-542 I
    J. Antibiot. 60(4): 251–255, 2007 THE JOURNAL OF ORIGINAL ARTICLE ANTIBIOTICS Urukthapelstatin A, a Novel Cytotoxic Substance from Marine-derived Mechercharimyces asporophorigenens YM11-542 I. Fermentation, Isolation and Biological Activities Yoshihide Matsuo, Kaneo Kanoh, Takao Yamori, Hiroaki Kasai, Atsuko Katsuta, Kyoko Adachi, Kazuo Shin-ya, Yoshikazu Shizuri Received: December 22, 2006 / Accepted: March 9, 2007 © Japan Antibiotics Research Association Abstract Urukthapelstatin A, a novel cyclic peptide, was isolated from the cultured mycelia of marine-derived Thermoactinomycetaceae bacterium Mechercharimyces asporophorigenens YM11-542. The peptide was purified by solvent extraction, silica gel chromatography, ODS flash chromatography, and finally by preparative HPLC. Urukthapelstatin A dose-dependently inhibited the growth of human lung cancer A549 cells with an IC50 value of 12 nM. Urukthapelstatin A also showed potent cytotoxic activity against a human cancer cell line panel. Keywords urukthapelstatin A, cyclic peptide, cytotoxic, Thermoactinomyces Introduction In the course of screening for new antibiotic compounds from marine-derived isolates, Mechercharimyces asporophorigenens YM11-542 was found to produce a Fig. 1 Structures of urukthapelstatin A (1), novel anticancer compound, urukthapelstatin A (1, Fig. 1), mechercharstatin (2; former name, mechercharmycin) and which was determined by a spectral analysis and chemical YM-216391 (3). degradation to be a unique cyclic peptide. This peptide possessed potent cytotoxicity against human cancer cell Y. Matsuo (Corresponding author), K. Kanoh, H. Kasai, A. K. Shin-ya: Chemical Biology Team, Biological Information Katsuta, K. Adachi, Y. Shizuri: Marine Biotechnology Institute Research Center (BIRC), National Institute of Advanced Co. Ltd., 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan, Industrial Science and Technology (AIST), 2-42 Aomi, Koto-ku, E-mail: [email protected] Tokyo 135-0064, Japan T.
    [Show full text]
  • Wo 2010/096550 A2
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date 26 August 2010 (26.08.2010) WO 2010/096550 A2 (51) International Patent Classification: (74) Agents: SALIWANCHIK, David, R. et al; Saliwanchik, C12N 1/20 (2006.01) A61K 35/74 (2006.01) Lloyd & Saliwanchik, P.O. Box 142950, Gainesville, FL A23C 9/123 (2006.01) C12R 1/225 (2006.01) 32614-2950 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2010/024575 kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, (22) International Filing Date: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, 18 February 2010 (18.02.2010) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (26) Publication Language: English ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (30) Priority Data: NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, 61/153,5 16 18 February 2009 (18.02.2009) US SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, 61/297,480 22 January 2010 (22.01 .2010) US TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicant (for all designated States except US): UNI¬ (84) Designated States (unless otherwise indicated, for every VERSITY OF FLORIDA RESEARCH FOUNDA¬ kind of regional protection available): ARIPO (BW, GH, TION, INC.
    [Show full text]
  • Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure
    Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure Authors: Suzanne L. Ishaq, Stephen P. Johnson, Zach J. Miller, Erik A. Lehnhoff, Sarah Olivo, Carl J. Yeoman, and Fabian D. Menalled The final publication is available at Springer via http://dx.doi.org/10.1007/s00248-016-0861-2. Ishaq, Suzanne L. , Stephen P. Johnson, Zach J. Miller, Erik A. Lehnhoff, Sarah Olivo, Carl J. Yeoman, and Fabian D. Menalled. "Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure." Microbial Ecology 73, no. 2 (February 2017): 417-434. DOI: 10.1007/s00248-016-0861-2. Made available through Montana State University’s ScholarWorks scholarworks.montana.edu Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure 1,2 & 2 & 3 & 4 & Suzanne L. Ishaq Stephen P. Johnson Zach J. Miller Erik A. Lehnhoff 1 1 2 Sarah Olivo & Carl J. Yeoman & Fabian D. Menalled 1 Department of Animal and Range Sciences, Montana State University, P.O. Box 172900, Bozeman, MT 59717, USA 2 Department of Land Resources and Environmental Sciences, Montana State University, P.O. Box 173120, Bozeman, MT 59717, USA 3 Western Agriculture Research Center, Montana State University, Bozeman, MT, USA 4 Department of Entomology, Plant Pathology and Weed Science, New Mexico State University, Las Cruces, NM, USA Abstract Farming practices affect the soil microbial commu- then individual farm. Living inoculum-treated soil had greater nity, which in turn impacts crop growth and crop-weed inter- species richness and was more diverse than sterile inoculum- actions.
    [Show full text]
  • Melghirimyces Thermohalophilus Sp. Nov., a Thermoactinomycete Isolated from an Algerian Salt Lake
    International Journal of Systematic and Evolutionary Microbiology (2013), 63, 1717–1722 DOI 10.1099/ijs.0.043760-0 Melghirimyces thermohalophilus sp. nov., a thermoactinomycete isolated from an Algerian salt lake Ammara Nariman Addou,1,2 Peter Schumann,3 Cathrin Spro¨er,3 Amel Bouanane-Darenfed,2 Samia Amarouche-Yala,4 Hocine Hacene,2 Jean-Luc Cayol1 and Marie-Laure Fardeau1 Correspondence 1Aix-Marseille Universite´, Universite´ du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marie-Laure Fardeau Marseille Cedex 09, France [email protected] 2Laboratoire de Biologie Cellulaire et Mole´culaire (e´quipe de Microbiologie), Universite´ des sciences et de la technologie, Houari Boume´die`nne, Bab Ezzouar, Algiers, Algeria 3Leibniz Institut DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany 4Centre de Recherche Nucle´aire d’Alger (CRNA), Algeria A novel filamentous bacterium, designated Nari11AT, was isolated from soil collected from a salt lake named Chott Melghir, located in north-eastern Algeria. The strain is an aerobic, halophilic, thermotolerant, Gram-stain-positive bacterium, growing at NaCl concentrations between 5 and 20 % (w/v) and at 43–60 6C and pH 5.0–10.0. The major fatty acids were iso-C15 : 0, anteiso- C15 : 0 and iso-C17 : 0. The DNA G+C content was 53.4 mol%. LL-Diaminopimelic acid was the diamino acid of the peptidoglycan. The major menaquinone was MK-7, but MK-6 and MK-8 were also present in trace amounts. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and three unidentified phospholipids.
    [Show full text]
  • Phylogenomic Analysis Supports the Ancestral Presence of LPS-Outer Membranes in the Firmicutes
    Phylogenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes. Luisa Cs Antunes, Daniel Poppleton, Andreas Klingl, Alexis Criscuolo, Bruno Dupuy, Céline Brochier-Armanet, Christophe Beloin, Simonetta Gribaldo To cite this version: Luisa Cs Antunes, Daniel Poppleton, Andreas Klingl, Alexis Criscuolo, Bruno Dupuy, et al.. Phy- logenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes.. eLife, eLife Sciences Publication, 2016, 5, pp.e14589. 10.7554/eLife.14589.020. pasteur-01362343 HAL Id: pasteur-01362343 https://hal-pasteur.archives-ouvertes.fr/pasteur-01362343 Submitted on 8 Sep 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License RESEARCH ARTICLE Phylogenomic analysis supports the ancestral presence of LPS-outer membranes in the Firmicutes Luisa CS Antunes1†, Daniel Poppleton1†, Andreas Klingl2, Alexis Criscuolo3, Bruno Dupuy4, Ce´ line Brochier-Armanet5, Christophe Beloin6, Simonetta Gribaldo1* 1Unite´ de
    [Show full text]
  • Deciphering the Composition and Functional Profile of the Microbial Communities in Chinese Moutai Liquor Starters
    Downloaded from orbit.dtu.dk on: Oct 03, 2021 Deciphering the composition and functional profile of the microbial communities in Chinese Moutai liquor starters Gan, Shu Heng; Yang, Fan; Sahu, Sunil Kumar; Luo, Ru Ye; Liao, Shui Lin; Wang, He Yu; Jin, Tao; Wang, Li; Zhang, Peng Fan; Liu, Xin Total number of authors: 14 Published in: Frontiers in Microbiology Link to article, DOI: 10.3389/fmicb.2019.01540 Publication date: 2019 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Gan, S. H., Yang, F., Sahu, S. K., Luo, R. Y., Liao, S. L., Wang, H. Y., Jin, T., Wang, L., Zhang, P. F., Liu, X., Xu, J., Xu, J., Wang, Y. Y., & Liu, H. (2019). Deciphering the composition and functional profile of the microbial communities in Chinese Moutai liquor starters. Frontiers in Microbiology, 10, [1540]. https://doi.org/10.3389/fmicb.2019.01540 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
    [Show full text]
  • Genome Diversity of Spore-Forming Firmicutes MICHAEL Y
    Genome Diversity of Spore-Forming Firmicutes MICHAEL Y. GALPERIN National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894 ABSTRACT Formation of heat-resistant endospores is a specific Vibrio subtilis (and also Vibrio bacillus), Ferdinand Cohn property of the members of the phylum Firmicutes (low-G+C assigned it to the genus Bacillus and family Bacillaceae, Gram-positive bacteria). It is found in representatives of four specifically noting the existence of heat-sensitive vegeta- different classes of Firmicutes, Bacilli, Clostridia, Erysipelotrichia, tive cells and heat-resistant endospores (see reference 1). and Negativicutes, which all encode similar sets of core sporulation fi proteins. Each of these classes also includes non-spore-forming Soon after that, Robert Koch identi ed Bacillus anthracis organisms that sometimes belong to the same genus or even as the causative agent of anthrax in cattle and the species as their spore-forming relatives. This chapter reviews the endospores as a means of the propagation of this orga- diversity of the members of phylum Firmicutes, its current taxon- nism among its hosts. In subsequent studies, the ability to omy, and the status of genome-sequencing projects for various form endospores, the specific purple staining by crystal subgroups within the phylum. It also discusses the evolution of the violet-iodine (Gram-positive staining, reflecting the pres- Firmicutes from their apparently spore-forming common ancestor ence of a thick peptidoglycan layer and the absence of and the independent loss of sporulation genes in several different lineages (staphylococci, streptococci, listeria, lactobacilli, an outer membrane), and the relatively low (typically ruminococci) in the course of their adaptation to the saprophytic less than 50%) molar fraction of guanine and cytosine lifestyle in a nutrient-rich environment.
    [Show full text]