Hints on Growing Clivia
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New Insights Into the Microbiota of Moth Pests
International Journal of Molecular Sciences Review New Insights into the Microbiota of Moth Pests Valeria Mereghetti, Bessem Chouaia and Matteo Montagna * ID Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, 20122 Milan, Italy; [email protected] (V.M.); [email protected] (B.C.) * Correspondence: [email protected]; Tel.: +39-02-5031-6782 Received: 31 August 2017; Accepted: 14 November 2017; Published: 18 November 2017 Abstract: In recent years, next generation sequencing (NGS) technologies have helped to improve our understanding of the bacterial communities associated with insects, shedding light on their wide taxonomic and functional diversity. To date, little is known about the microbiota of lepidopterans, which includes some of the most damaging agricultural and forest pests worldwide. Studying their microbiota could help us better understand their ecology and offer insights into developing new pest control strategies. In this paper, we review the literature pertaining to the microbiota of lepidopterans with a focus on pests, and highlight potential recurrent patterns regarding microbiota structure and composition. Keywords: symbiosis; bacterial communities; crop pests; forest pests; Lepidoptera; next generation sequencing (NGS) technologies; diet; developmental stages 1. Introduction Insects represent the most successful taxa of eukaryotic life, being able to colonize almost all environments, including Antarctica, which is populated by some species of chironomids (e.g., Belgica antarctica, Eretmoptera murphyi, and Parochlus steinenii)[1,2]. Many insects are beneficial to plants, playing important roles in seed dispersal, pollination, and plant defense (by feeding upon herbivores, for example) [3]. On the other hand, there are also damaging insects that feed on crops, forest and ornamental plants, or stored products, and, for these reasons, are they considered pests. -
Pests and Diseases
Clivia assassins – pests and diseases Clivia is really quite a resistant little genus, with only a few serious pests and diseases that can be life threatening . Others, though not lethal, can seriously affect a plant’s appearance and growth . General hygienic culture conditions like good drainage, removal of infected plants and material and a spray programme can successfully prevent these pests and diseases from becoming a serious problem . Just a note of caution when working with toxic chemicals: Read the instructions of all chemicals before use! Make sure that chemicals can be mixed without detrimental effects to treated plants – if not stated as mixable, test it first on a few plants before you treat your whole collection . Plant pests Lily borer (Brithys crini, Brithys pancratii) Brithys species, also known as Amaryllis caterpillars, are serious pests amongst members of the Amaryllidaceae. They target Crinum, Cyrthanthus, Haemanthus, Nerine, Amaryllis and Clivia, to name a few. This destructive pest has three to four generations in nature annually and if a severe infestation occurs it can destroy plants within a few days. The caterpillar Breakfast! The yellow and brown-black banding is easily distinguishable with its yellow and black or brown pattern of the Brithys caterpillar makes it easy to recognise . Only a mushy pseudo stem remains banding pattern. Young larvae emerge from a cluster of after Mr Caterpillar’s visit . Growing clivias eggs, usually on the underside (abaxial side) of leaves, and then start to tunnel into the leaf. Once inside, the larvae eat the soft tissue between the outer two epidermal cell layers, tunnelling their way towards the base of the leaf. -
Clivia Miniata Colour Mutations and Their Breeding
Clivia Miniata Colour Mutations and their Breeding I don’t profess to be geneticists or to know very much about the way in which colour is inherited in Clivia, but by taking a very simple approach of line breeding and a lot of trial and error I have managed to produce true breeding lines of Clivia. This knowledge will be valuable to any breeder wishing to perpetuate a particular colour mutation. In the best interest of Clivia I have decided to write this article and attempt to share what I have learnt with others. It is my opinion that for a particular colour mutation to be inherited by sexual reproduction, a pathway has to occur i.e. the genes or other factors influencing the colour would have to line up. A simple way in which to view the chromosomes and the loci on which these chromosomes have mutated is by taking two rulers and placing them side by side, each representing different plants with similar colour mutations and only if both plants have mutated on the same point of the chromosomes (rulers) would the mutation be transferred to the off spring by sexual reproduction. Plants are relatively easy to line breed / inbreed because of the fact that they can be self- pollinated. Unfortunately, mechanisms are in place in plants that inhibit self-pollination and so more effort is required to perpetuate these colour mutations. The best-known colour mutation in Clivia is the yellow mutation occurring in Clivia miniata. Even here just because two plants are yellow doesn’t necessarily mean they have mutated at the same loci on the chromosome. -
Gut Bacterial Communities of Two Insect Species Feeding on Toxic Plants Are Dominated by Enterococcus Sp
fmicb-07-01005 June 24, 2016 Time: 16:20 # 1 ORIGINAL RESEARCH published: 28 June 2016 doi: 10.3389/fmicb.2016.01005 The Generalist Inside the Specialist: Gut Bacterial Communities of Two Insect Species Feeding on Toxic Plants Are Dominated by Enterococcus sp. Cristina Vilanova1,2, Joaquín Baixeras1, Amparo Latorre1,2,3* and Manuel Porcar1,2* 1 Cavanilles Institute of Biodiversity and Evolutionary Biology, Universitat de València, Valencia, Spain, 2 Institute for Integrative Systems Biology (I2SysBio), University of Valencia-CSIC, Valencia, Spain, 3 Unidad Mixta de Investigación en Genómica y Salud, Centro Superior de Investigación en Salud Pública, Valencia, Spain Some specialist insects feed on plants rich in secondary compounds, which pose a major selective pressure on both the phytophagous and the gut microbiota. However, microbial communities of toxic plant feeders are still poorly characterized. Here, we Edited by: Mark Alexander Lever, show the bacterial communities of the gut of two specialized Lepidoptera, Hyles ETH Zürich, Switzerland euphorbiae and Brithys crini, which exclusively feed on latex-rich Euphorbia sp. and Reviewed by: alkaloid-rich Pancratium maritimum, respectively. A metagenomic analysis based on Virginia Helena Albarracín, CONICET, Argentina high-throughput sequencing of the 16S rRNA gene revealed that the gut microbiota Jeremy Dodsworth, of both insects is dominated by the phylum Firmicutes, and especially by the common California State University, gut inhabitant Enterococcus sp. Staphylococcus sp. are also found in H. euphorbiae San Bernardino, USA though to a lesser extent. By scanning electron microscopy, we found a dense ring- *Correspondence: Manuel Porcar shaped bacterial biofilm in the hindgut of H. euphorbiae, and identified the most [email protected]; prominent bacterium in the biofilm as Enterococcus casseliflavus through molecular Amparo Latorre [email protected] techniques. -
The Green Scene Brighten Winter with a Clivia Plant
Marion County Extension 210 N. Iowa St. Knoxville, IA 50138 641.842.2014 [email protected] February, 2019 Volume 17, Issue 2 The Green Scene Save a tree! Send your email address to [email protected] to receive this publication via e-mail. Brighten Winter with a Clivia Plant By Richard Jauon Flowers are one of the best antidotes to the icy winds of winter, and growing a houseplant that buds and blooms inside while all is dormant outside is particularly satisfying. This winter, as an alternative to the brightly blooming azaleas, chrysanthemums or traditional holiday plants, consider growing a clivia plant. What is a clivia? Clivia or kaffir lily (Clivia spp.) is a herbaceous flowering plant native to South Africa. Plants have long, arching, strap-like leaves (similar to an amaryllis) and produce dense clusters of trumpet-shaped flowers atop 18 to 24 inch stems. The flowers of Clivia minia- ta are typically orange with yellow eyes or centers. However, there are also several rare and expensive yellow-flowering cultivars. While clivias are not winter hardy in Iowa, they are excellent, low maintenance houseplants. How do I get a clivia to bloom? Clivias need a rest period of six to 12 weeks in fall and winter to initiate flower bud development. Temperatures dur- ing this time should be 40 to 55 degrees Fahrenheit. A guest bedroom, porch or a partially heated garage (temperatures must remain above 35 F) may be suitable plant locations. Water sparingly (about once a month). When flower stalks appear, move plants to a slightly warmer location and begin to water more frequently. -
Biodiversity and Ecology of Critically Endangered, Rûens Silcrete Renosterveld in the Buffeljagsrivier Area, Swellendam
Biodiversity and Ecology of Critically Endangered, Rûens Silcrete Renosterveld in the Buffeljagsrivier area, Swellendam by Johannes Philippus Groenewald Thesis presented in fulfilment of the requirements for the degree of Masters in Science in Conservation Ecology in the Faculty of AgriSciences at Stellenbosch University Supervisor: Prof. Michael J. Samways Co-supervisor: Dr. Ruan Veldtman December 2014 Stellenbosch University http://scholar.sun.ac.za Declaration I hereby declare that the work contained in this thesis, for the degree of Master of Science in Conservation Ecology, is my own work that have not been previously published in full or in part at any other University. All work that are not my own, are acknowledge in the thesis. ___________________ Date: ____________ Groenewald J.P. Copyright © 2014 Stellenbosch University All rights reserved ii Stellenbosch University http://scholar.sun.ac.za Acknowledgements Firstly I want to thank my supervisor Prof. M. J. Samways for his guidance and patience through the years and my co-supervisor Dr. R. Veldtman for his help the past few years. This project would not have been possible without the help of Prof. H. Geertsema, who helped me with the identification of the Lepidoptera and other insect caught in the study area. Also want to thank Dr. K. Oberlander for the help with the identification of the Oxalis species found in the study area and Flora Cameron from CREW with the identification of some of the special plants growing in the area. I further express my gratitude to Dr. Odette Curtis from the Overberg Renosterveld Project, who helped with the identification of the rare species found in the study area as well as information about grazing and burning of Renosterveld. -
(Tribe Haemantheae) Inferred from Plastid and Nuclear Non-Coding DNA Sequences
Plant Syst. Evol. 244: 141–155 (2004) DOI 10.1007/s00606-003-0085-z Generic relationships among the baccate-fruited Amaryllidaceae (tribe Haemantheae) inferred from plastid and nuclear non-coding DNA sequences A. W. Meerow1, 2 and J. R. Clayton1 1 USDA-ARS-SHRS, National Germplasm Repository, Miami, Florida, USA 2 Fairchild Tropical Garden, Miami, Florida, USA Received October 22, 2002; accepted September 3, 2003 Published online: February 12, 2004 Ó Springer-Verlag 2004 Abstract. Using sequences from the plastid trnL-F Key words: Amaryllidaceae, Haemantheae, geo- region and nrDNA ITS, we investigated the phy- phytes, South Africa, monocotyledons, DNA, logeny of the fleshy-fruited African tribe Haeman- phylogenetics, systematics. theae of the Amaryllidaceae across 19 species representing all genera of the tribe. ITS and a Baccate fruits have evolved only once in the combined matrix produce the most resolute and Amaryllidaceae (Meerow et al. 1999), and well-supported tree with parsimony analysis. Two solely in Africa, but the genera possessing main clades are resolved, one comprising the them have not always been recognized as a monophyletic rhizomatous genera Clivia and Cryp- monophyletic group. Haemanthus L. and tostephanus, and a larger clade that unites Haemanthus and Scadoxus as sister genera to an Gethyllis L. were the first two genera of the Apodolirion/Gethyllis subclade. One of four group to be described (Linneaus 1753). Her- included Gethyllis species, G. lanuginosa, resolves bert (1837) placed Haemanthus (including as sister to Apodolirion with ITS. Relationships Scadoxus Raf.) and Clivia Lindl. in the tribe among the Clivia species are not in agreement with Amaryllidiformes, while Gethyllis was classi- a previous published phylogeny. -
Towards a Molecular Understanding of the Biosynthesis of Amaryllidaceae Alkaloids in Support of Their Expanding Medical Use
Int. J. Mol. Sci. 2013, 14, 11713-11741; doi:10.3390/ijms140611713 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Review Towards a Molecular Understanding of the Biosynthesis of Amaryllidaceae Alkaloids in Support of Their Expanding Medical Use Adam M. Takos and Fred Rook * Plant Biochemistry Laboratory, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +45-3533-3343; Fax: +45-3533-3300. Received: 28 April 2013; in revised form: 26 May 2013 / Accepted: 27 May 2013 / Published: 31 May 2013 Abstract: The alkaloids characteristically produced by the subfamily Amaryllidoideae of the Amaryllidaceae, bulbous plant species that include well know genera such as Narcissus (daffodils) and Galanthus (snowdrops), are a source of new pharmaceutical compounds. Presently, only the Amaryllidaceae alkaloid galanthamine, an acetylcholinesterase inhibitor used to treat symptoms of Alzheimer’s disease, is produced commercially as a drug from cultivated plants. However, several Amaryllidaceae alkaloids have shown great promise as anti-cancer drugs, but their further clinical development is restricted by their limited commercial availability. Amaryllidaceae species have a long history of cultivation and breeding as ornamental bulbs, and phytochemical research has focussed on the diversity in alkaloid content and composition. In contrast to the available pharmacological and phytochemical data, ecological, physiological and molecular aspects of the Amaryllidaceae and their alkaloids are much less explored and the identity of the alkaloid biosynthetic genes is presently unknown. An improved molecular understanding of Amaryllidaceae alkaloid biosynthesis would greatly benefit the rational design of breeding programs to produce cultivars optimised for the production of pharmaceutical compounds and enable biotechnology based approaches. -
Growing the Miracle Cl Ivia
The newly discovered miracle clivi a 'Gardening from the arid north-western Cape, Clivia mirabilis flowering at Kirstenbosch. Photo: Graham Duncan. Growing the miracle cl ivia by Graham Duncan, Kirstenbosch The recent new addition to the genus Clivia brought about large quantities of water and sustain the plant over the long a great flurry of excitement among ardent followers of clivias. and dry, harsh summer. The rounded seeds of C. mirabilis The new species was discovered by game guard Johannes are relatively small and similar to those of C. nobilis. Afrika in a nature reserve in a remote canyon of the The flowers of C. mirabilis cannot realistically be described Bokkeveld escarpment near Nieuwoudtville in the north as being any more attractive than the best forms of the three western Cape. It was brought to the attention of botanists at other tubular-flowered species C. caulescens, e. gardenii and Kirstenbosch by Wessel Pretorius, officer in charge of the e. nobilis, and its most appealing features in my view, apart reserve. from the reddish-orange perianth, are the long, dark red The region where it was found is semi-arid, with a arching flower stalks and bright orangy-red ovaries. Mediterranean-type climate, very different from the subtrop Flowering rather late in the year, from mid-October to ical environment in which the four other Clivia species November, the berries ripen rapidly in only four months by (e. caulescens, C. gardenii, C. miniata and C. nobilis) are mid- to late March, just before the onset of the first autumn found in eastern and north-eastern South Africa. -
BRUNSVIGIA ORIENTALIS Candelabra flower, Koningskandelaar, Perdespookbossie Amaryllidaceae
SNR FACT SHEET BRUNSVIGIA ORIENTALIS Candelabra flower, Koningskandelaar, Perdespookbossie Amaryllidaceae Late summer in Steenbok Park sees the emergence of the spectacular crimson Candelabra flower or Brunsvigia orientalis which grows in scattered colonies in coastal sand. The bud of this large bulb pushes up through the sand on its sturdy stem before a leaf can be seen, and produces up to 40 flowers in a head shaped like a rounded candelabra. As the flowers fade the ovaries enlarge and become papery and eventually the flower stem breaks away and the flower head is blown about, tumbling over the ground and scattering its seeds. These ‘balls’ blowing in the wind no doubt give rise to the Afrikaans name Perdespookbossie. The plant was initially called Amaryllis orientalis, but in 1753 Lorenz Heister, a botanist and professor of medicine at the University of Helmstädt, renamed it Brunsvigia in honour of his patron the Duke of Brunswick. Karl Wilhelm Ferdinand (1735-1806), a cultured and benevolent despot, promoted the study of plants. The bulb had been sent to Germany in 1748 by Cape Governor, Ryk Tulbagh, who was very interested in the flora and fauna of the Cape and regularly sent plants and stuffed animals to Europe. Brunsvigias are deciduous and have adapted to the dry period of the year by resting underground. The large flower heads appear shortly before the rainy season. Sunbirds searching for nectar in the tubular flowers are their chief pollinators. Once the seeds have been scattered they germinate very quickly, giving the seedling a full rainy season to develop sufficiently to withstand its first dry season underground. -
MAPEAMENTO DOS SÍTIOS DE Dnar 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL
EMMANUELLY CALINA XAVIER RODRIGUES DOS SANTOS MAPEAMENTO DOS SÍTIOS DE DNAr 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL. RECIFE-PE 2015 i EMMANUELLY CALINA XAVIER RODRIGUES DOS SANTOS MAPEAMENTO DOS SÍTIOS DE DNAr 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL. Tese apresentada ao Programa de Pós-Graduação em Botânica da Universidade Federal Rural de Pernambuco como parte dos requisitos para obtenção do título de Doutora em Botânica. Orientador: Prof. Dr. Reginaldo de Carvalho Dept° de Genética/Biologia, Área de Genética/UFRPE Co-orientador: Prof. Dr. Leonardo Pessoa Felix Dept° de Fitotecnia, UFPB RECIFE-PE 2015 ii MAPEAMENTO DOS SÍTIOS DE DNAr 5S E 45S E ORGANIZAÇÃO DA CROMATINA EM REPRESENTANTES DA FAMÍLIA AMARYLLIDACEAE JAUME ST.-HIL. Emmanuelly Calina Xavier Rodrigues dos Santos Tese defendida e _________________ pela banca examinadora em ___/___/___ Presidente da Banca/Orientador: ______________________________________________ Dr. Reginaldo de Carvalho (Universidade Federal Rural de Pernambuco – UFRPE) Comissão Examinadora: Membros titulares: ______________________________________________ Dra. Ana Emília de Barros e Silva (Universidade Federal da Paraíba – UFPB) ______________________________________________ Dra. Andrea Pedrosa Harand (Universidade Federal de Pernambuco – UFPE) ______________________________________________ Dr. Felipe Nollet Medeiros de Assis (Universidade Federal da Paraíba – UFPB) ______________________________________________ Dr. Marcelo Guerra (Universidade Federal de Pernambuco – UFPE) Suplentes: ______________________________________________ Dra. Lânia Isis Ferreira Alves (Universidade Federal da Paraíba – UFPB) ______________________________________________ Dra. Sônia Maria Pereira Barreto (Universidade Federal de Pernambuco – UFRPE) iii A minha família, em especial ao meu pai José Geraldo Rodrigues dos Santos que sempre foi o meu maior incentivador e a quem responsabilizo o meu amor pela docência. -
Final Policy Review: Alternative Risk Management Measures to Import Lilium Spp
International plant protection convention 14_EWGCutFlowers_2014_June Final policy review Lilium spp. Agenda Item: 4.1 ------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------- Final policy review: Alternative risk management measures to import Lilium spp. cut flowers from Taiwan December 2013 International plant protection convention 14_EWGCutFlowers_2014_June Final policy review Lilium spp. Agenda Item: 4.1 ------------------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------- © Commonwealth of Australia Ownership of intellectual property rights Unless otherwise noted, copyright (and any other intellectual property rights, if any) in this publication is owned by the Commonwealth of Australia (referred to as the Commonwealth). Creative Commons licence All material in this publication is licensed under a Creative Commons Attribution 3.0 Australia Licence, except for content supplied by third parties, photographic images, logos, and the Commonwealth Coat of Arms. Creative Commons Attribution 3.0 Australia Licence is a standard form licence agreement that allows you to copy, distribute, transmit and adapt this publication provided that you attribute the work. A summary of the licence terms is available from creativecommons.org/licenses/by/3.0/au/deed.en.