UQCRC2 Antibody

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Efficient Professional Protein and Antibody Platforms UQCRC2 Antibody Basic information: Catalog No.: UPA60311 Source: Rabbit Size: 50ul/100ul Clonality: Polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: Protein affinity purified. Useful Information: WB:1:1000-2000 ICC:1:50-1:200 Applications: IHC:1:50-1:200 FC:1:50-1:100 Reactivity: Human, Mouse, Rat Specificity: This antibody recognizes UQCRC2 protein. Immunogen: Recombinant protein within human UQCRC2 aa 100-350. Cytochrome c is a well characterized, mobile electron transport protein that is essential to energy conversion in all aerobic organisms. Cytochrome b as- sociates with cytochrome c subunit 1 and the Rieske protein to form complex III (also designated cytochrome bc1 complex), which is involved in cel- lular respiration. Ubiquinol cytochrome c reductase (UQCRFS1), also re- ferred to as Rieske iron-sulfur protein, represents an important subunit of Description: complex III of the mitochondrial respiratory chain that transfers electrons from ubiquinol to cytochrome c. The UQCRFS1 complex is made up of 3 respiratory subunits (cytochrome b, cytochrome c1, Rieske protein), 2 core proteins, and 6 low-molecular weight proteins. Ubiquinol cytochrome-c reductase complex core protein 2 (UQCRC2) represents one of the core proteins of UQCRFS1, and it is required for the assembly of the complex. Uniprot: P22695(Human) Q9DB77(Mouse) P32551(Rat) BiowMW: 48 kDa Buffer: 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution. ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). ICC staining UQCRC2 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). ICC staining UQCRC2 in SiHa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue). Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .

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Autism Multiplex Family with 16P11.2P12.2 Microduplication Syndrome in Monozygotic Twins and Distal 16P11.2 Deletion in Their Brother
European Journal of Human Genetics (2012) 20, 540–546 & 2012 Macmillan Publishers Limited All rights reserved 1018-4813/12 www.nature.com/ejhg ARTICLE Autism multiplex family with 16p11.2p12.2 microduplication syndrome in monozygotic twins and distal 16p11.2 deletion in their brother Anne-Claude Tabet1,2,3,4, Marion Pilorge2,3,4, Richard Delorme5,6,Fre´de´rique Amsellem5,6, Jean-Marc Pinard7, Marion Leboyer6,8,9, Alain Verloes10, Brigitte Benzacken1,11,12 and Catalina Betancur*,2,3,4 The pericentromeric region of chromosome 16p is rich in segmental duplications that predispose to rearrangements through non-allelic homologous recombination. Several recurrent copy number variations have been described recently in chromosome 16p. 16p11.2 rearrangements (29.5–30.1 Mb) are associated with autism, intellectual disability (ID) and other neurodevelopmental disorders. Another recognizable but less common microdeletion syndrome in 16p11.2p12.2 (21.4 to 28.5–30.1 Mb) has been described in six individuals with ID, whereas apparently reciprocal duplications, studied by standard cytogenetic and ﬂuorescence in situ hybridization techniques, have been reported in three patients with autism spectrum disorders. Here, we report a multiplex family with three boys affected with autism, including two monozygotic twins carrying a de novo 16p11.2p12.2 duplication of 8.95 Mb (21.28–30.23 Mb) characterized by single-nucleotide polymorphism array, encompassing both the 16p11.2 and 16p11.2p12.2 regions. The twins exhibited autism, severe ID, and dysmorphic features, including a triangular face, deep-set eyes, large and prominent nasal bridge, and tall, slender build. The eldest brother presented with autism, mild ID, early-onset obesity and normal craniofacial features, and carried a smaller, overlapping 16p11.2 microdeletion of 847 kb (28.40–29.25 Mb), inherited from his apparently healthy father.
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Airway Inflammation Mitochondrial Dysfunction Increases Allergic
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UQCRC2 Antibody Order 021-34695924 [email protected] Support 400-6123-828 50Ul [email protected] 100 Ul √ √ Web
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The Human Genome Project
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Datasheet Blank Template
SAN TA C RUZ BI OTEC HNOL OG Y, INC . UQCRC2 (H-135): sc-292924 BACKGROUND RECOMMENDED SECONDARY REAGENTS Cytochrome c is a well characterized, mobile electron transport protein that To ensure optimal results, the following support (secondary) reagents are is essential to energy conversion in all aerobic organisms. Cytochrome b asso - recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004 ciates with cytochrome c subunit 1 and the Rieske protein to form complex III (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat anti- (also designated cytochrome bc1 complex), which is involved in cellular res - rabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™ piration. Ubiquinol cytochrome c reductase (UQCRFS1), also referred to as Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: Rieske iron-sulfur protein, represents an important subunit of complex III of sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- the mitochondrial respiratory chain that transfers electrons from ubiquinol to itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). cytochrome c. The UQCRFS1 complex is made up of three respiratory sub - 3) Immunofluorescence: use goat anti-rabbit IgG-FITC: sc-2012 (dilution units (cytochrome b, cytochrome c1, Rieske protein), two core proteins, and range: 1:100-1:400) or goat anti-rabbit IgG-TR: sc-2780 (dilution range: six low-molecular weight proteins. Ubiquinol cytochrome-c reductase com plex 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. core protein 2 (UQCRC2) represents one of the core proteins of UQCRFS1, and it is required for the assembly of the complex.
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Clinical Utility Gene Card For: 16P12.2 Microdeletion
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