Efficient Professional Protein and Antibody Platforms UQCRC2 Antibody Basic information: Catalog No.: UPA60311 Source: Rabbit Size: 50ul/100ul Clonality: Polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: Protein affinity purified. Useful Information: WB:1:1000-2000 ICC:1:50-1:200 Applications: IHC:1:50-1:200 FC:1:50-1:100 Reactivity: Human, Mouse, Rat Specificity: This antibody recognizes UQCRC2 protein. Immunogen: Recombinant protein within human UQCRC2 aa 100-350. Cytochrome c is a well characterized, mobile electron transport protein that is essential to energy conversion in all aerobic organisms. Cytochrome b as- sociates with cytochrome c subunit 1 and the Rieske protein to form com- plex III (also designated cytochrome bc1 complex), which is involved in cel- lular respiration. Ubiquinol cytochrome c reductase (UQCRFS1), also re- ferred to as Rieske iron-sulfur protein, represents an important subunit of Description: complex III of the mitochondrial respiratory chain that transfers electrons from ubiquinol to cytochrome c. The UQCRFS1 complex is made up of 3 res- piratory subunits (cytochrome b, cytochrome c1, Rieske protein), 2 core proteins, and 6 low-molecular weight proteins. Ubiquinol cytochrome-c re- ductase complex core protein 2 (UQCRC2) represents one of the core pro- teins of UQCRFS1, and it is required for the assembly of the complex. Uniprot: P22695(Human) Q9DB77(Mouse) P32551(Rat) BiowMW: 48 kDa Buffer: 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data:

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.

ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

ICC staining UQCRC2 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

ICC staining UQCRC2 in SiHa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]