Tendon Fibroblasts
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Review the Cellular Matrix: a Feature of Tensile Bearing Dense Soft
Histol Histopathol (2002) 17: 523-537 Histology and http://www.hh.um.es Histopathology Cellular and Molecular Biology Review The cellular matrix: a feature of tensile bearing dense soft connective tissues I.K. Lo, S. Chi, T. Ivie, C.B. Frank and J.B. Rattner Joint Injury and Arthritis Research Group, University of Calgary, Calgary, Canada S u m m a r y. The term connective tissue encompasses a recent studies using a variety of microscopic and indirect d iverse group of tissues that reside in diff e r e n t immunofluorescence techniques suggest that these e nvironments and must support a spectrum of apparently diverse groups of tissues, which are mechanical functions. Although the extracellular matrix characterized by a spectrum of functions and in vivo of these tissues is well described, the cellular mechanical environments, are organized around similar architecture of these tissues and its relationship to tissue architectural principles. function has only recently become the focus of study. It The purpose of this rev i ew is to highlight recent n ow appears that tensile-bearing dense connective d evelopments in our understanding of the cellular tissues may be a specific class of connective tissues that architecture of dense soft connective tissues and to display a common cellular organization characterized by discuss their functional implications. It will become fusiform cells with cytoplasmic projections and ga p clear that a 3-dimensional cellular arrangement, a junctions. These cells with their cellular projections are “cellular matrix”, consisting of fusiform cells with organised into a complex 3-dimensional network leading cytoplasmic projections and gap junctions may define a to a phy s i c a l l y, chemically and electrically connected s p e c i fic class of hy p o c e l l u l a r, tensile-bearing, dense cellular matrix. -
Comparative Multi-Scale Hierarchical Structure of the Tail, Plantaris, and Achilles Tendons in 2 the Rat 3 Andrea H
bioRxiv preprint doi: https://doi.org/10.1101/396309; this version posted August 20, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Comparative Multi-scale Hierarchical Structure of the Tail, Plantaris, and Achilles Tendons in 2 the Rat 3 Andrea H. Lee, Dawn M. Elliott* 4 Department of Biomedical Engineering, University of Delaware 5 *Corresponding author. Tel.: +1 302 831 1295. E-mail address: [email protected] (D.M. Elliott). 6 7 Keyword: Tendon, Hierarchical Structure, Multi-scale, Imaging 8 9 10 Abstract (500 words) 11 Rodent tendons are widely used to study human pathology, such as tendinopathy and 12 repair, and to address fundamental physiological questions about development, growth, and 13 remodeling. However, how the gross morphology and the multi-scale hierarchical structure of 14 rat tendons, such as the tail, plantaris, and Achillles tendons, compare to that of human 15 tendons are unknown. In addition, there remains disagreement about terminology and 16 definitions. Specifically, the definition of fascicle and fiber are often dependent on the diameter 17 size and not their characteristic features, which impairs the ability to compare across species 18 where the size of the fiber and fascicle might change with animal size and tendon function. 19 Thus, the objective of the study was to select a single species that is widely used for tendon 20 research (rat) and tendons with varying mechanical functions (tail, plantaris, Achilles) to 21 evaluate the hierarchical structure at multiple length scales. -
Establishment and Investigation of Tendon-Derived Cell Lines Immortalized by the Human Telomerase Reverse Transcriptase Gene
Aus der Chirurgischen Klinik und Poliklinik – Innenstadt, der Ludwig-Maximilians-Universität München Direktor: Prof. Dr. med. Wolf Mutschler Establishment and investigation of tendon- derived cell lines immortalized by the human telomerase reverse transcriptase gene. DISSERTATION zum Erwerb des Doktorgrades der Medizin an der Medizinischen Fakultät der Ludwig-Maximilians-Universität zu München vorgelegt von: Sophia Amina Poppe aus München, im Jahr 2010 Mit Genehmigung der Medizinischen Fakultät der Universität München Berichterstatter: Prof. Dr. med. Matthias Schieker Mitberichterstatter: Prof. Dr. Günther Eißner Prof. Dr. Peter Müller Mitbetreuung durch die promovierten Mitarbeiter: Dr. rer. nat. Denitsa Docheva Dekan: Prof. Dr. med. Dr. h.c. M. Reiser FACR, FRCR Tag der mündlichen Prüfung: 08. Juli 2010 1. INTRODUCTION......................................................................................................................... 4 1.1. General background......................................................................................................... 4 1.1.1. Anatomical and molecular structure of tendons.............................................. 4 1.1.1.1. Tendinous tissue................................................................................................ 4 1.1.1.2. Osteotendinous junction - enthesis............................................................ 5 1.1.1.3. Myotendinous junction................................................................................... 7 1.1.2. Development of tendons...................................................................................... -
Biomaterials in Tendon and Skeletal Muscle Tissue Engineering: Current Trends and Challenges
materials Review Biomaterials in Tendon and Skeletal Muscle Tissue Engineering: Current Trends and Challenges Megane Beldjilali-Labro 1,†, Alejandro Garcia Garcia 1,†, Firas Farhat 1,† ID , Fahmi Bedoui 2, Jean-François Grosset 1, Murielle Dufresne 1 and Cécile Legallais 1,* ID 1 CNRS, UMR 7338, Biomécanique-Bioingénierie, Sorbonne Universités, Université de Technologie de Compiègne, 60200 Compiègne, France; [email protected] (M.B.-L.); [email protected] (A.G.G.); fi[email protected] (F.F.); [email protected] (J.-F.G.); [email protected] (M.D.) 2 CNRS FRE 2012, Laboratoire Roberval, Sorbonne Universités, Université de Technologie de Compiègne, 60200 Compiègne, France; [email protected] * Correspondence: [email protected] † These authors contributed equally to this work. Received: 31 May 2018; Accepted: 25 June 2018; Published: 29 June 2018 Abstract: Tissue engineering is a promising approach to repair tendon and muscle when natural healing fails. Biohybrid constructs obtained after cells’ seeding and culture in dedicated scaffolds have indeed been considered as relevant tools for mimicking native tissue, leading to a better integration in vivo. They can also be employed to perform advanced in vitro studies to model the cell differentiation or regeneration processes. In this review, we report and analyze the different solutions proposed in literature, for the reconstruction of tendon, muscle, and the myotendinous junction. They classically rely on the three pillars of tissue engineering, i.e., cells, biomaterials and environment (both chemical and physical stimuli). We have chosen to present biomimetic or bioinspired strategies based on understanding of the native tissue structure/functions/properties of the tissue of interest. -
Nomina Histologica Veterinaria, First Edition
NOMINA HISTOLOGICA VETERINARIA Submitted by the International Committee on Veterinary Histological Nomenclature (ICVHN) to the World Association of Veterinary Anatomists Published on the website of the World Association of Veterinary Anatomists www.wava-amav.org 2017 CONTENTS Introduction i Principles of term construction in N.H.V. iii Cytologia – Cytology 1 Textus epithelialis – Epithelial tissue 10 Textus connectivus – Connective tissue 13 Sanguis et Lympha – Blood and Lymph 17 Textus muscularis – Muscle tissue 19 Textus nervosus – Nerve tissue 20 Splanchnologia – Viscera 23 Systema digestorium – Digestive system 24 Systema respiratorium – Respiratory system 32 Systema urinarium – Urinary system 35 Organa genitalia masculina – Male genital system 38 Organa genitalia feminina – Female genital system 42 Systema endocrinum – Endocrine system 45 Systema cardiovasculare et lymphaticum [Angiologia] – Cardiovascular and lymphatic system 47 Systema nervosum – Nervous system 52 Receptores sensorii et Organa sensuum – Sensory receptors and Sense organs 58 Integumentum – Integument 64 INTRODUCTION The preparations leading to the publication of the present first edition of the Nomina Histologica Veterinaria has a long history spanning more than 50 years. Under the auspices of the World Association of Veterinary Anatomists (W.A.V.A.), the International Committee on Veterinary Anatomical Nomenclature (I.C.V.A.N.) appointed in Giessen, 1965, a Subcommittee on Histology and Embryology which started a working relation with the Subcommittee on Histology of the former International Anatomical Nomenclature Committee. In Mexico City, 1971, this Subcommittee presented a document entitled Nomina Histologica Veterinaria: A Working Draft as a basis for the continued work of the newly-appointed Subcommittee on Histological Nomenclature. This resulted in the editing of the Nomina Histologica Veterinaria: A Working Draft II (Toulouse, 1974), followed by preparations for publication of a Nomina Histologica Veterinaria. -
Rebuilding Tendons: a Concise Review on the Potential of Dermal Fibroblasts
cells Review Rebuilding Tendons: A Concise Review on the Potential of Dermal Fibroblasts Jin Chu 1 , Ming Lu 2, Christian G. Pfeifer 1,3 , Volker Alt 1,3 and Denitsa Docheva 1,* 1 Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, 93053 Regensburg, Germany; [email protected] (J.C.); [email protected] (C.G.P.); [email protected] (V.A.) 2 Department of Orthopaedic Surgery, First Affiliated Hospital of Dalian Medical University, Dalian 116023, China; [email protected] 3 Department of Trauma Surgery, University Regensburg Medical Centre, 93053 Regensburg, Germany * Correspondence: [email protected]; Tel.: +49-(0)941-943-1605 Received: 29 June 2020; Accepted: 2 September 2020; Published: 8 September 2020 Abstract: Tendons are vital to joint movement by connecting muscles to bones. Along with an increasing incidence of tendon injuries, tendon disorders can burden the quality of life of patients or the career of athletes. Current treatments involve surgical reconstruction and conservative therapy. Especially in the elderly population, tendon recovery requires lengthy periods and it may result in unsatisfactory outcome. Cell-mediated tendon engineering is a rapidly progressing experimental and pre-clinical field, which holds great potential for an alternative approach to established medical treatments. The selection of an appropriate cell source is critical and remains under investigation. Dermal fibroblasts exhibit multiple similarities to tendon cells, suggesting they may be a promising cell source for tendon engineering. Hence, the purpose of this review article was in brief, to compare tendon to dermis tissues, and summarize in vitro studies on tenogenic differentiation of dermal fibroblasts. -
Tendon Extracellular Matrix Remodeling and Defective Cell Polarization in the Presence of Collagen VI Mutations
cells Article Tendon Extracellular Matrix Remodeling and Defective Cell Polarization in the Presence of Collagen VI Mutations Manuela Antoniel 1,2, Francesco Traina 3,4, Luciano Merlini 5 , Davide Andrenacci 1,2, Domenico Tigani 6, Spartaco Santi 1,2, Vittoria Cenni 1,2, Patrizia Sabatelli 1,2,*, Cesare Faldini 7 and Stefano Squarzoni 1,2 1 CNR-Institute of Molecular Genetics “Luigi Luca Cavalli-Sforza”-Unit of Bologna, 40136 Bologna, Italy; [email protected] (M.A.); [email protected] (D.A.); [email protected] (S.S.); [email protected] (V.C.); [email protected] (S.S.) 2 IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy 3 Ortopedia-Traumatologia e Chirurgia Protesica e dei Reimpianti d’Anca e di Ginocchio, Istituto Ortopedico Rizzoli di Bologna, 40136 Bologna, Italy; [email protected] 4 Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, Università Degli Studi Di Messina, 98122 Messina, Italy 5 Department of Biomedical and Neuromotor Sciences, University of Bologna, 40123 Bologna, Italy; [email protected] 6 Department of Orthopedic and Trauma Surgery, Ospedale Maggiore, 40133 Bologna, Italy; [email protected] 7 1st Orthopaedic and Traumatologic Clinic, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy; [email protected] * Correspondence: [email protected]; Tel.: +39-051-6366755; Fax: +39-051-4689922 Received: 20 December 2019; Accepted: 7 February 2020; Published: 11 February 2020 Abstract: Mutations in collagen VI genes cause two major clinical myopathies, Bethlem myopathy (BM) and Ullrich congenital muscular dystrophy (UCMD), and the rarer myosclerosis myopathy. In addition to congenital muscle weakness, patients affected by collagen VI-related myopathies show axial and proximal joint contractures, and distal joint hypermobility, which suggest the involvement of tendon function. -
Brown Adipose Tissue: New Challenges for Prevention of Childhood Obesity
nutrients Review Brown Adipose Tissue: New Challenges for Prevention of Childhood Obesity. A Narrative Review Elvira Verduci 1,2,*,† , Valeria Calcaterra 2,3,† , Elisabetta Di Profio 2,4, Giulia Fiore 2, Federica Rey 5,6 , Vittoria Carlotta Magenes 2, Carolina Federica Todisco 2, Stephana Carelli 5,6,* and Gian Vincenzo Zuccotti 2,5,6 1 Department of Health Sciences, University of Milan, 20146 Milan, Italy 2 Department of Pediatrics, Vittore Buzzi Children’s Hospital, University of Milan, 20154 Milan, Italy; [email protected] (V.C.); elisabetta.diprofi[email protected] (E.D.P.); giulia.fi[email protected] (G.F.); [email protected] (V.C.M.); [email protected] (C.F.T.); [email protected] (G.V.Z.) 3 Pediatric and Adolescent Unit, Department of Internal Medicine, University of Pavia, 27100 Pavia, Italy 4 Department of Animal Sciences for Health, Animal Production and Food Safety, University of Milan, 20133 Milan, Italy 5 Department of Biomedical and Clinical Sciences “L. Sacco”, University of Milan, 20157 Milan, Italy; [email protected] 6 Pediatric Clinical Research Center Fondazione Romeo ed Enrica Invernizzi, University of Milan, 20157 Milan, Italy * Correspondence: [email protected] (E.V.); [email protected] (S.C.) † These authors contributed equally to this work. Abstract: Pediatric obesity remains a challenge in modern society. Recently, research has focused on the role of the brown adipose tissue (BAT) as a potential target of intervention. In this review, we Citation: Verduci, E.; Calcaterra, V.; revised preclinical and clinical works on factors that may promote BAT or browning of white adipose Di Profio, E.; Fiore, G.; Rey, F.; tissue (WAT) from fetal age to adolescence. -
Estudo Histológico E Biomecânico Da Tendinopatia Induzida Por Injeções Seriadas De Colagenase: Novo Modelo Experimental No
CÉSAR DE CÉSAR NETTO Estudo histológico e biomecânico da tendinopatia induzida por injeções seriadas de colagenase: novo modelo experimental no tendão do calcâneo de coelhos Tese apresentada à Faculdade de Medicina da Universidade de São Paulo para obtenção do Título de Doutor em Ciências Programa de Ortopedia e Traumatologia Orientador: Prof. Dr. Túlio Diniz Fernandes São Paulo 2017 Dados Internacionais de Catalogação na Publicação (CIP) Preparada pela Biblioteca da Faculdade de Medicina da Universidade de São Paulo reprodução autorizada pelo autor Cesar Netto, Cesar de Estudo histológico e biomecânico da tendinopatia induzida por injeções seriadas de colagenase : novo modelo experimental no tendão do calcâneo de coelhos/ Cesar de Cesar Netto. -- São Paulo, 2017. Tese(doutorado)--Faculdade de Medicina da Universidade de São Paulo. Programa de Ortopedia e Traumatologia. Orientador: Túlio Diniz Fernandes. Descritores: 1.Tendinopatia 2.Tendão do calcâneo 3.Modelos animais 4.Tendinopatia induzida quimicamente 5.Colagenase bacteriana 6.Coelhos USP/FM/DBD-105/17 Dedicatória Dedicatória À minha amada esposa Sabrina, amor da minha vida, que sempre esteve ao meu lado, me apoiando nos momentos mais difíceis. À minha filha Manuela, razão do meu viver. À minha filha Juliana, que logo estará conosco e nos trará ainda mais alegria. Aos meus amados pais Luiz Edmundo e Maria Amélia, pelo amor e carinho, e por proporcionarem todas as oportunidades da minha vida. À minha querida irmã Marcela, eterna companheira, que me presenteou com meus amados sobrinhos Gianluca e Sofia. Ao meu saudoso avô Osvaldo, melhor amigo. A Deus, por me dar saúde e sempre me guiar pelos melhores caminhos. -
Tendon Healing with Allogenic Fibroblast and Static Magnetic Field in Rabbit Model
IJVS 2015; 10(2); Serial No:23 IRANIAN JOURNAL OF VETERINARY SURGERY (IJVS) WWW.IVSA.IR Tendon Healing with Allogenic Fibroblast and Static Magnetic Field in Rabbit Model Amin Bigham-Sadegh*1, Setare Ghasemi 2, Iraj Karimi 3, Pezhman Mirshokraei 4, Hasan Nazari 5, Ahmad Oryan 6 Abstract Objectives- Tendons are integral parts of musculoskeletal system and are subjected to injury. Fibroblast is used in tendon healing, however, there is no proved and reported result regarding concurrent use of allogenic fibroblast with static magnetic field in tendon healing. In addition, there are some studies done on the effect of magnetic fields on tendon healing but the results are antithesis. The aim of this study is to evaluate the effect of simultaneous application of fibroblast and magnetic field on tendon healing in rabbit model. Design- Experimental study. Animals- Eighteen female rabbits, 15 months old and weighing 3.0±0.5 kg were used in this study. Procedures- Two legs of eighteen rabbits were divided into 6 groups. After skin incision, superficial flexor tendon was exposed and cut transversely and then sutured. In control group tendon injury were created in right and left legs and sutured in bunnell mayer suturing technique. In culture media substance group after tendon injury in two legs, 0.5 cc culture substance was injected in the injured tendon area in two legs. In fibroblast group, fibroblast cells were injected in the tendon injured area in both legs. Then all injuries legs were dressed up, a piece of magnet was placed in the surrounding bandage of the left leg for 7 days and right legs were left empty. -
White Adipocyte Plasticity in Physiology and Disease
cells Review White Adipocyte Plasticity in Physiology and Disease Ewa Bielczyk-Maczynska Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; [email protected] Received: 19 October 2019; Accepted: 21 November 2019; Published: 25 November 2019 Abstract: Cellular plasticity is a transformation of a terminally differentiated cell into another cell type, which has been long known to occur in disease and regeneration. However, white adipocytes (fat cells) have only recently been observed to undergo different types of cellular plasticity. Adipocyte transdifferentiation into myofibroblasts and cancer-associated fibroblasts occurs in fibrosis and cancer, respectively. On the other hand, reversible adipocyte dedifferentiation into adipocyte progenitor cells (preadipocytes) has been demonstrated in mammary gland and in dermal adipose tissue. Here we discuss the research on adipocyte plasticity, including the experimental approaches that allowed to detect and study it, the current state of the knowledge, major research questions which remain to be addressed, and the advances required to stimulate adipocyte plasticity research. In the future, the knowledge of the molecular mechanisms of adipocyte plasticity can be utilized both to prevent adipocyte plasticity in disease and to stimulate it for use in regenerative medicine. Keywords: cell plasticity; adipocytes; fat; cell dedifferentiation; cell transdifferentiation; cell differentiation 1. Introduction In the traditional view of cell differentiation, cells follow a differentiation trajectory in discrete developmental stages, beginning with stem cells and culminating with a terminally differentiated state [1]. The terminally differentiated state is thought to be permanent as the cells can no longer transfer into other cell fates. However, in many systems the phenomenon of cellular plasticity, which is a transformation of a cellular phenotype beginning with a terminally differentiated cell, has been described not only in pathologies like cancer, but also during physiological processes [2]. -
Extracellular Matrix Regulates Fibroblast Heterogeneity and Tumorigenesis
University of Pennsylvania ScholarlyCommons Publicly Accessible Penn Dissertations 2017 Extracellular Matrix Regulates Fibroblast Heterogeneity And Tumorigenesis Diana Leigh Avery University of Pennsylvania, [email protected] Follow this and additional works at: https://repository.upenn.edu/edissertations Part of the Cell Biology Commons, Oncology Commons, and the Pharmacology Commons Recommended Citation Avery, Diana Leigh, "Extracellular Matrix Regulates Fibroblast Heterogeneity And Tumorigenesis" (2017). Publicly Accessible Penn Dissertations. 2173. https://repository.upenn.edu/edissertations/2173 This paper is posted at ScholarlyCommons. https://repository.upenn.edu/edissertations/2173 For more information, please contact [email protected]. Extracellular Matrix Regulates Fibroblast Heterogeneity And Tumorigenesis Abstract Heterogeneous activated fibroblasts that deposit and remodel extracellular matrix (ECM) comprise desmoplasia, a key regulator of tumor development. The divergent outcomes in response to varied therapies targeting intratumoral desmoplasia underscore the pressing need to delineate the intricate role of a heterogeneous stroma in tumorigenesis. Fibroblast activation protein (FAP) and alpha-smooth muscle actin (αSMA) identify distinct, yet overlapping, activated fibroblast subsets in myriad tumor types, fibrosis, and wound healing. FAPHi reactive fibroblasts and αSMAHi myofibroblasts can exert divergent influences on tumor progression. However, the factors that drive this phenotypic heterogeneity and the