THE ORBITRAP: a NEW MASS SPECTROMETER Qizhi Hua, Robert J

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THE ORBITRAP: A NEW MASS SPECTROMETER Qizhi Hua, Robert J. Nolla, Hongyan Lia, Alexander Makarovb, Mark Hardmanc and R. Graham Cooksa a Purdue University, b Thermo Electron (Bremen), c Thermo Electron (San Jose) Supported by NSF Grant CHE-0216239, ONR, and Thermo Electron Corporation The Orbitrap is a new mass spectrometer based on a new type of mass analyzer invented by Makarov. Features of the Orbitrap at its present stage of development include high mass resolution (up to 150 000), large space charge capacity, high mass accuracy (2–5 ppm), a mass/charge range of at least 6000, and dynamic range greater than 1000 Figure 2. The experimental Orbitrap mass spectrometer. Ions are produced by the electrospray Figure 1. Cutaway view of the Orbitrap mass analyzer. ion source at the extreme left. Ions then proceed through the source, collision quadrupole, Ions are injected at the point indicated by the red arrow selection quadrupole and then pass into the storage quadrupole. The storage quadrupole serves with velocity perpendicular to the long axis of the as an ion accumulator and buncher, allowing a pulsed mass analyzer such as the Orbitrap to be Orbitrap (the z-axis). Injection at a point displaced from coupled to a continuous source like an electrospray ionization source. After accumulation and z=0 gives the ions potential energy in the z-direction. bunching in the storage quadrupole, the exit lens (‘Lens 1’) is pulsed low, the ion bunches Ion injection at this point on the z-potential is analogous traverse the ion transfer lens system and are injected into the Orbitrap mass analyzer (shown to pulling back a pendulum bob and then releasing it to end-on). oscillate. In this case, the frequency of motion is dependent on ion mass and independent of its velocity. J. Mass Spectrom. 2005; 40: 430–443.

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