Journal of Systematics and Evolution

Total Page:16

File Type:pdf, Size:1020Kb

Journal of Systematics and Evolution Journal of Systematics and Evolution wileyonlinelibrary.com/journal/jse J S E J S E Journal of Systematics and Evolution Journal of Systematics and Evolution Volume 53 Number 2 March 2015 N D I N O I D E A E A R U I C R A I R O I M D E A E D A N T H O N I O I E D Andropogoninae Arundineae E Mi r A i a ieae E Molinieae INVITED REVIEW craire C Anthistiriinae c H h L Saccharinae n O 117 Robert J. Soreng, Paul M. Peterson, Konstantin Romaschenko, Gerrit Davidse, Fernando O. Zuloaga, Emmet J. Judziewicz, Germainiinae S R Rottboell org e I a a D Tarciso S. Filgueiras, Jerrold I. Davis, and Osvaldo Morrone A worldwide phylogenetic classifi cation of the Poaceae (Gramineae) e Ischaeminae hinae e O Isachneae tteinae Hubbard Danthonieae nae I Centropodieae Triraphideae D Dimeriinae Co E ii Uniolinae Chio nae Volume 53 Volume Number 2 March 2015 Pages 117–202 Eragrostidinae robolinae A RESEARCH ARTICLE ae Coicina e Eragr Zoysiinae E a E na e Spo 138 Robert J. Soreng, Lynn J. Gillespie, Hidehisa Koba, Ekaterina Boudko, and Roger D. Bull Molecular and morphological A Tripsacinaec n Zoy Aeluropodi ininae Arthraxoninaehnin o riodiin E Arundinelleae g T iinae evidence for a new grass genus, Dupontiopsis (Poaceae tribe Poeae subtribe Poinae s.l.), endemic to alpine Japan, and implications for e o ae D a p Eleus n I Arthropogoninae e o Orcutt ae the reticulate origin of Dupontia and Arctophila within Poinae s.l. r n nae O d Gouinii C Otachyrii n I C Ctenii goninae A y LETTER TO THE EDITOR N Trichoneurinopo P nae n m A aspalina o Gy l 163 Wei-Ning Bai and Da-Yong Zhang Small effective population size in microrefugia? P d P a Farragininaedinae anicinae e p o oti s Melinidinae a n Per P t nae RESEARCH ARTICLES Cench e Tripogoninae rin e Neurachninaeae a Pappophori 166 Xiao-Qin Wu, Pei-Xing Li, Xiao-Fang Deng, and Dian-Xiang Zhang Distyly and cryptic heteromorphic self-incompatibility a e Dichan e Traginae in Mussaenda macrophylla (Rubiaceae) thelini c nae nae ilarii i H 179 Xiao-Qin Li, Qin Zuo, Min Li, Si He, Yu Jia, and You-Fang Wang Phylogenetic relationships and species delimitation among the Boivinellin iinae ae n lenberg Anthep Muh a horinae taxa with propagula in Pseudotaxiphyllum (Plagiotheciaceae, Bryophyta) Scleropogoninae P Gynerieae 191 Xin-Bo Zhang, Xiang-Ming Ma, Bai-Yun Wang, Xiang-Hui Ma, and Zhi-Wen Wang Selective pressure on synonymous codon Joinvillea Boutelouinae Cyperochloeae Ecdeiocolea usage in mammalian protein-encoding genes PACMAD Monanthochloinae E Thysanolaeneae 196 Gao Chen, Wei-Chang Gong, Jia Ge, Yang Niu, Xin Zhang, Bruce L. Dunn, and Wei-Bang Sun Comparison of fl oral properties A Strep E Centotheceae tochaeteae E and breeding system in dimorphic Buddleja delavayi (Scrophulariaceae) Anomo D chloe A ae I P h are a E Steyermarkochloeae BOP e O Guaduellieae Tristachyideae E D D Puel Atractocarpeae I Zeugiteae A I E O E T thieae Streptogyneae A E O S h D Chasman rhart I E I L Aristideae Phyllorachideaeeae E R D O H Zizaniinae I Ory A A Scolochloinae R C Loliinae Oryzinae E O A I O Arundinariinae D Sesleriinae L H C I M Buergersiochloinae h Oly E P P O O lo ylidinae arianinae Ammochloinae U r O Z o Dact lyr N p C inae Y P e husqueinae A la liinae a Cynosurinae P o e a e e Arthrostylidiin E R s inae s Cover illustration: New phylotaxonomy of grasses, includes 12 subfamilies (Anomochlooideae, Pharoideae, and t u Guad A G b O ParaphoHolc E ro e m Bambusinae a Racemobambosinae D Puelioideae forming the basal lineages; Oryzoideae, Bambusoideae, and Pooideae forming the BOP clade; and u Poinae B uinae p liina H I 2 Mi e Melo ickeliinae ae irinae O Aristidoideae, Panicoideae, Arundinoideae, Micraioideae, Danthonioideae, and Chloridoideae forming the (P A S o ae B ea in Lygeeaerachyel canninae U PACMAD clade), 51 tribes, and 80 subtribes evolving clockwise. Triangles are proportional in height to e Nardea B eanthina Trit Phaenospermateae Brylkinieae M rostid Meliceae Col Stipeae Amp nae Diar Brachyp A the size of the taxon. See Soreng et al., pp. 117–137 in this issue. Ag Brizinae nae hi ytreae B inae nae nae C Calothecinae Aveninae e ei h rheneae elodesmea l d o oxant ritici r. r G T r. 1 Phalaridi (A Ho v Anth odieae en Bromeae a orreyochlo e ty pe) T Littledaleeae e P O O I D E A E ISSN 1674-4918 Volume 53 Number 2 March 2015 CN 11-5779/Q JJSE_53(2)_Cover4_1.inddSE_53(2)_Cover4_1.indd 1 119/03/159/03/15 99:52:52 AAMM Journal of Systematics JSE and Evolution doi: 10.1111/jse.12150 Invited Review A worldwide phylogenetic classification of the Poaceae (Gramineae) Robert J. Soreng1, Paul M. Peterson1*, Konstantin Romaschenko1, Gerrit Davidse2, Fernando O. Zuloaga3, Emmet J. Judziewicz4, Tarciso S. Filgueiras5, Jerrold I. Davis6, and Osvaldo Morrone3,7 1Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC 20013-7012, USA 2Missouri Botanical Garden, P.O. Box 299, St. Louis, MO 63166-0299, USA 3Instituto de Botanica Darwinion, Labarden 200, San Isidro B1642HYD, Buenos Aires, Argentina 4Department of Biology and Museum of Natural History, University of Wisconsin-Stevens Point, WI 54481, USA 5Instituto de Botanica,^ Secretaria do Meio Ambiente, Avenida Miguel Stefano 3687, Agua Funda, S~ao Paulo CEP, 04301-902, Brazil 6Department of Plant Biology, Cornell University, 412 Mann Library Building, Ithaca, NY 14853, USA 7Deceased *Author for correspondence. E-mail: [email protected]. Tel.: 1-202-633-0975. Fax: 1-202-786-2653. Received 5 February 2015; Accepted 25 February 2015; Article first published online 23 March 2015 Abstract Based on recent molecular and morphological studies we present a modern worldwide phylogenetic classification of the Æ 12074 grasses and place the 771 grass genera into 12 subfamilies (Anomochlooideae, Aristidoideae, Arundinoideae, Bambusoideae, Chloridoideae, Danthonioideae, Micraioideae, Oryzoideae, Panicoi- deae, Pharoideae, Puelioideae, and Pooideae), 6 supertribes (Andropogonodae, Arundinarodae, Bambusodae, Panicodae, Poodae, Triticodae), 51 tribes (Ampelodesmeae, Andropogoneae, Anomochloeae, Aristideae, Arundinarieae, Arundineae, Arundinelleae, Atractocarpeae, Bambuseae, Brachyelytreae, Brachypodieae, Bromeae, Brylkinieae, Centotheceae, Centropodieae, Chasmanthieae, Cynodonteae, Cyperochloeae, Danthonieae, Diarrhe- neae, Ehrharteae, Eragrostideae, Eriachneae, Guaduellieae, Gynerieae, Hubbardieae, Isachneae, Littledaleeae, Lygeeae, Meliceae, Micraireae, Molinieae, Nardeae, Olyreae, Oryzeae, Paniceae, Paspaleae, Phaenospermateae, Phareae, Phyllorachideae, Poeae, Steyermarkochloeae, Stipeae, Streptochaeteae, Streptogyneae, Thysanolaeneae, Triraphideae, Tristachyideae, Triticeae, Zeugiteae, and Zoysieae), and 80 subtribes (Aeluropodinae, Agrostidinae, Airinae, Ammochloinae, Andropogoninae, Anthephorinae, Anthistiriinae, Anthoxanthinae, Arthraxoninae, Arthro- pogoninae, Arthrostylidiinae, Arundinariinae, Aveninae, Bambusinae, Boivinellinae, Boutelouinae, Brizinae, Buergersiochloinae, Calothecinae, Cenchrinae, Chionachninae, Chusqueinae, Coicinae, Coleanthinae, Cotteinae, Cteniinae, Cynosurinae, Dactylidinae, Dichantheliinae, Dimeriinae, Duthieinae, Eleusininae, Eragrostidinae, Farragininae, Germainiinae, Gouiniinae, Guaduinae, Gymnopogoninae, Hickeliinae, Hilariinae, Holcinae, Hordeinae, Ischaeminae, Loliinae, Melinidinae, Melocanninae, Miliinae, Monanthochloinae, Muhlenbergiinae, Neurachninae, Olyrinae, Orcuttiinae, Oryzinae, Otachyriinae, Panicinae, Pappophorinae, Parapholiinae, Parianinae, Paspalinae, Perotidinae, Phalaridinae, Poinae, Racemobambosinae, Rottboelliinae, Saccharinae, Scleropogoninae, Scolochloi- nae, Sesleriinae, Sorghinae, Sporobolinae, Torreyochloinae, Traginae, Trichoneurinae, Triodiinae, Tripogoninae, Tripsacinae, Triticinae, Unioliinae, Zizaniinae, and Zoysiinae). In addition, we include a radial tree illustrating the hierarchical relationships among the subtribes, tribes, and subfamilies. We use the subfamilial name, Oryzoideae, over Ehrhartoideae because the latter was initially published as a misplaced rank, and we circumscribe Molinieae to include 13 Arundinoideae genera. The subtribe Calothecinae is newly described and the tribe Littledaleeae is new at that rank. Key words: classification, DNA, Gramineae, grasses, morphology, phylogeny, Poaceae, subfamily, subtribe, tribe. In 1991, most of the authors of this paper attended the 42nd Ehrhartoideae, and Pharoideae was published (Judziewicz American Institute of Biological Sciences Annual Meeting in et al., 2000). Subsequently, the Chloridoideae (Peterson et al., San Antonio, Texas to discuss the possibility of creating a large 2001), Pooideae (Soreng et al., 2003), and the Panicoideae, digital database that would include nomenclature, taxonomy, Aristidoideae, Arundinoideae, and Danthonioideae (Zuloaga synonymy, original publications, type collections, secondary et al., 2003) were printed. Within each of these, provisional references using accepted names, and distribution by country but now woefully out of date treatments, we included our of all New World grasses. Nine years later the first hard copy best estimate of the generic classification of the tribes and treatment of subfamilies Anomochlooideae, Bambusoideae, subtribes for each subfamily. The online database for the March 2015 | Volume 53 | Issue 2 | 117–137 © 2015 Institute of Botany, Chinese Academy of Sciences 118 Soreng et al. classification of New World grasses was first posted as a Peterson et al. (2010a, 2014c). A radial phylogenetic tree separate file in Tropicos in 2005. Since that time it has been produced in FigTree version 1.4.2
Recommended publications
  • Poaceae: Bambusoideae) Christopher Dean Tyrrell Iowa State University
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 2008 Systematics of the neotropical woody bamboo genus Rhipidocladum (Poaceae: Bambusoideae) Christopher Dean Tyrrell Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Botany Commons Recommended Citation Tyrrell, Christopher Dean, "Systematics of the neotropical woody bamboo genus Rhipidocladum (Poaceae: Bambusoideae)" (2008). Retrospective Theses and Dissertations. 15419. https://lib.dr.iastate.edu/rtd/15419 This Thesis is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Systematics of the neotropical woody bamboo genus Rhipidocladum (Poaceae: Bambusoideae) by Christopher Dean Tyrrell A thesis submitted to the graduate faculty in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Major: Ecology and Evolutionary Biology Program of Study Committee: Lynn G. Clark, Major Professor Dennis V. Lavrov Robert S. Wallace Iowa State University Ames, Iowa 2008 Copyright © Christopher Dean Tyrrell, 2008. All rights reserved. 1457571 1457571 2008 ii In memory of Thomas D. Tyrrell Festum Asinorum iii TABLE OF CONTENTS ABSTRACT iv CHAPTER 1. GENERAL INTRODUCTION 1 Background and Significance 1 Research Objectives 5 Thesis Organization 6 Literature Cited 6 CHAPTER 2. PHYLOGENY OF THE BAMBOO SUBTRIBE 9 ARTHROSTYLIDIINAE WITH EMPHASIS ON RHIPIDOCLADUM Abstract 9 Introduction 10 Methods and Materials 13 Results 19 Discussion 25 Taxonomic Treatment 26 Literature Cited 31 CHAPTER 3.
    [Show full text]
  • ARTICLE Floristic and Characterization of Grassland Vegetation at a Granitic Hill in Southern Brazil Robberson Bernal Setubal1* and Ilsi Iob Boldrini2
    Revista Brasileira de Biociências Brazilian Journal of Biosciences http://www.ufrgs.br/seerbio/ojs ISSN 1980-4849 (on-line) / 1679-2343 (print) ARTICLE Floristic and characterization of grassland vegetation at a granitic hill in Southern Brazil Robberson Bernal Setubal1* and Ilsi Iob Boldrini2 Received: March 31 2009 Received after revision: November 19 2009 Accepted: November 20 2009 Available online at http://www.ufrgs.br/seerbio/ojs/index.php/rbb/article/view/1209 ABSTRACT: (Floristic and characterization of grasslands vegetation of a granite hill in Southern Brazil). A floristic and vegeta- tion study was carried out in the grassland formations at Morro São Pedro, Porto Alegre municipality, Rio Grande do Sul State, a granitic elevation area, so far poorly surveyed, that cradles important natural vegetation remnants of the region. After the study, which lasted from April 2005 to March 2009, we found 497 angiosperm species, distributed in four main grassland formation types: dry grassland, rocky grassland, humid grassland and wetlands. Among the species list three species are noteworthy: Alstroemeria albescens, a new species for the science, Lepuropethalon spathulatum, a new record for Southern Brazil, and Thrasyopsis juergensii, a new record for the Pampa biome. Based on our results and on support from other papers we concluded that ca. 65% of the grassland species present in the granitic hills of the region belong to seven main botanical families (Astera- ceae, Poaceae, Fabaceae, Cyperaceae, Rubiaceae, Verbenaceae and Apiaceae). The species belonging to these families are also determining in the vegetation phytophysiognomical and structural composition, so that cespitous grasses predominate in the landscape, shaping a continuous gramineous layer.
    [Show full text]
  • Poaceae: Bambusoideae: Bambuseae: Arthrostylidiinae) with the Northernmost Distribution of the Genus
    Phytotaxa 344 (1): 031–038 ISSN 1179-3155 (print edition) http://www.mapress.com/j/pt/ PHYTOTAXA Copyright © 2018 Magnolia Press Article ISSN 1179-3163 (online edition) https://doi.org/10.11646/phytotaxa.344.1.4 A new species of Merostachys (Poaceae: Bambusoideae: Bambuseae: Arthrostylidiinae) with the northernmost distribution of the genus EDUARDO RUIZ-SANCHEZ1,*, LYNN G. CLARK2, TERESA MEJÍA-SAULÉS3 & FRANCISCO LOREA- HERNÁNDEZ4 1 Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara. Camino Ing. Ramón Padilla Sánchez 2100, Nextipac, Zapopan, Jalisco 45110, Mexico., e-mail: [email protected] 2 Department of Ecology, Evolution, and Organismal Biology, 251 Bessey Hall, Iowa State University, Ames, Iowa 50011–4009, United States of America., e-mail: [email protected] 3 Red de Biología Evolutiva, Instituto de Ecología, A. C. Carretera antigua a Coatepec 351, Xalapa, 91070, Mexico., e-mail: [email protected] 4 Red de Biodiversidad y Sistemática, Instituto de Ecología, A. C. Carretera antigua a Coatepec 351, Xalapa, 91070, Mexico., e-mail: [email protected] *author for correspondence Abstract With 52 described species, Merostachys is the most diverse genus in the Arthrostylidiinae; 50 of the species are present in South America and only two, M. latifolia and M. pauciflora, are distributed in Central America and Mexico. Previous col- lections of vegetative Merostachys specimens from El Triunfo, Chiapas, Mexico, were identified as M. pauciflora. However, new flowering collections from the state of Tabasco, Mexico, allowed us to differentiate the Mexican populations from M. latifolia and M. pauciflora. A detailed study of samples from the Tabasco population, and a review of the previous collections from Chiapas, confirmed the existence of a new Merostachys species, which we here describe and illustrate as M.
    [Show full text]
  • Genome Sequence of the Progenitor of the Wheat D Genome Aegilops Tauschii Ming-Cheng Luo1*, Yong Q
    OPEN LETTER doi:10.1038/nature24486 Genome sequence of the progenitor of the wheat D genome Aegilops tauschii Ming-Cheng Luo1*, Yong Q. Gu2*, Daniela Puiu3*, Hao Wang4,5,6*, Sven O. Twardziok7*, Karin R. Deal1, Naxin Huo1,2, Tingting Zhu1, Le Wang1, Yi Wang1,2, Patrick E. McGuire1, Shuyang Liu1, Hai Long1, Ramesh K. Ramasamy1, Juan C. Rodriguez1, Sonny L. Van1, Luxia Yuan1, Zhenzhong Wang1,8, Zhiqiang Xia1, Lichan Xiao1, Olin D. Anderson2, Shuhong Ouyang2,8, Yong Liang2,8, Aleksey V. Zimin3, Geo Pertea3, Peng Qi4,5, Jeffrey L. Bennetzen6, Xiongtao Dai9, Matthew W. Dawson9, Hans-Georg Müller9, Karl Kugler7, Lorena Rivarola-Duarte7, Manuel Spannagl7, Klaus F. X. Mayer7,10, Fu-Hao Lu11, Michael W. Bevan11, Philippe Leroy12, Pingchuan Li13, Frank M. You13, Qixin Sun8, Zhiyong Liu8, Eric Lyons14, Thomas Wicker15, Steven L. Salzberg3,16, Katrien M. Devos4,5 & Jan Dvořák1 Aegilops tauschii is the diploid progenitor of the D genome of We conclude therefore that the size of the Ae. tauschii genome is about hexaploid wheat1 (Triticum aestivum, genomes AABBDD) and 4.3 Gb. an important genetic resource for wheat2–4. The large size and To assess the accuracy of our assembly, sequences of 195 inde- highly repetitive nature of the Ae. tauschii genome has until now pendently sequenced and assembled AL8/78 BAC clones8, which precluded the development of a reference-quality genome sequence5. contained 25,540,177 bp in 2,405 unordered contigs, were aligned to Here we use an array of advanced technologies, including ordered- Aet v3.0. Five contigs failed to align and six extended partly into gaps, clone genome sequencing, whole-genome shotgun sequencing, accounting for 0.25% of the total length of the contigs.
    [Show full text]
  • Taxonomy, Morphology and Palynology of Aegilops Vavilovii (Zhuk.) Chennav
    African Journal of Agricultural Research Vol. 5(20), pp. 2841-2849, 18 October, 2010 Available online at http://www.academicjournals.org/AJAR ISSN 1991-637X ©2010 Academic Journals Full Length Research Paper Taxonomy, morphology and palynology of Aegilops vavilovii (Zhuk.) Chennav. (Poaceae: Triticeae) Evren Cabi1* Musa Doan1 Hülya Özler2, Galip Akaydin3 and Alptekin Karagöz4 1Department of Biological Sciences, Faculty of Arts and Sciences, Middle East Technical University, Ankara, Turkey. 2Department of Biology, Faculty of Arts and Sciences, Sinop University, Sinop Turkey. 3Department of Biology Education, Hacettepe University, 06800 Ankara, Turkey. 4Department of Biology, Aksaray University, Aksaray, Turkey. Accepted 23 September, 2010 Aegilops vavilovii (Zhuk.) Chennav., a rare species, was collected from Southeast Anatolia, Turkey. During the field studies of the project “Taxonomic revision of Tribe Triticeae in Turkey”, Ae. vavilovii was accidentally recollected from three localities in anliurfa and Mardin provinces in 2007 and 2008, respectively. The main objective of this study is to shed light on the diagnostic characteristics of this rare species including its morphological, palynological and micro morphological features. Moreover, an emended and expanded description, distribution, phenology and ecology of this rare species are also provided. A. vavilovii and A. crassa are naturally found in the Southeastern part of Turkey and they share similar morphological features that caused a confused taxonomy. Pollen grains of A. vavilovii are heteropolar, monoporate and spheroidal (A/B: 1,13) typically as Poaceous. However, it generally, prefers clayish loam soils that are slightly alkaline (pH 7.7) with low organic content (1.54%). Although it is a rare species with very narrow area of distribution, very few samples have been represented in ex situ collections and the species has not been involved in any in situ conservation activities to save its genetic resources in Turkey.
    [Show full text]
  • Distribution and Diversity of Grass Species in Banni Grassland, Kachchh District, Gujarat, India
    Patel Yatin et al. IJSRR 2012, 1(1), 43-56 Research article Available online www.ijsrr.org ISSN: 2279-0543 International Journal of Scientific Research and Reviews Distribution and Diversity of Grass Species in Banni Grassland, Kachchh District, Gujarat, India 1* 2 3 Patel Yatin , Dabgar YB , and Joshi PN 1Shri Jagdish Prasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan 2R.R. Mehta Colg. of Sci. and C.L. Parikh College of Commerce, Palanpur, Banaskantha, Gujarat. 3Sahjeevan, 175-Jalaram Society, Vijay Nagar, Bhuj- Kachchh, Gujarat ABSTRACT: Banni, an internationally recognized unique grassland stretch of Western India. It is a predominantly flat land with several shallow depressions, which act as seasonal wetlands after monsoon and during winter its converts into sedge mixed grassland, an ideal dual ecosystem. An attempt was made to document ecology, biomass and community based assessment of grasses in Banni, we surveyed systematically and recorded a total of 49 herbaceous plant species, being used as fodder by livestock. In which, the maximum numbers of species (21 Nos.) were recorded in Echinocloa and Cressa habitat; followed by Sporobolus and Elussine habitat (20 species); and Desmostechia-Aeluropus and Cressa habiat (19 species). A total of 21 highest palatable species were recorded in Echinocloa-Cressa communities followed by Sporobolus-Elussine–Desmostechia (20 species and 18 palatable species) and Aeluropus–Cressa (19 species and 17 palatable species). For long-term conservation of Banni grassland, we also suggest a participatory co-management plan. KEY WORDS: Banni, Grassland, Palatability, Communities, Conservation. *Corresponding Author: Yatin Patel Research Scholar, Shri Jagdish Prasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan E-mail: [email protected] IJSRR 1(1) APRIL-JUNE 2012 Page 43 Patel Yatin et al.
    [Show full text]
  • Identification and Validation of Aeluropus Littoralis Reference Genes
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Springer - Publisher Connector Hashemi et al. J of Biol Res-Thessaloniki (2016) 23:18 DOI 10.1186/s40709-016-0053-8 Journal of Biological Research-Thessaloniki RESEARCH Open Access Identification and validation of Aeluropus littoralis reference genes for Quantitative Real‑Time PCR Normalization Seyyed Hamidreza Hashemi1, Ghorbanali Nematzadeh1†, Gholamreza Ahmadian2†, Ahad Yamchi3 and Markus Kuhlmann4* Abstract Background: The use of stably expressed genes as normalizers has crucial role in accurate and reliable expression analysis estimated by quantitative real-time polymerase chain reaction (qPCR). Recent studies have shown that, the expression levels of common housekeeping genes are varying in different tissues and experimental conditions. The genomic DNA contamination in RNA samples is another important factor that also influence the interpretation of the data obtained from qPCR. It is estimated that the gDNA contamination in gene expression analysis lead to an overes- timation of the RNA transcript level. The aim of this study was to validate the most stably expressed reference genes in two different tissues of Aeluropus littoralis—halophyte grass at salt stress and recovery condition. Also, a qPCR-based approach for monitoring contamination with gDNA was conducted. Results: Ten candidate reference genes participating in different biological processes were analyzed in four groups of samples including root and leaf tissues, salt stress and recovery condition. To determine the most stably expressed reference genes, three statistical methods (geNorm, NormFinder and BestKeeper) were applied. According to results obtained, ten candidate reference genes were ranked based on the stability of their expression.
    [Show full text]
  • Checklist Das Spermatophyta Do Estado De São Paulo, Brasil
    Biota Neotrop., vol. 11(Supl.1) Checklist das Spermatophyta do Estado de São Paulo, Brasil Maria das Graças Lapa Wanderley1,10, George John Shepherd2, Suzana Ehlin Martins1, Tiago Egger Moellwald Duque Estrada3, Rebeca Politano Romanini1, Ingrid Koch4, José Rubens Pirani5, Therezinha Sant’Anna Melhem1, Ana Maria Giulietti Harley6, Luiza Sumiko Kinoshita2, Mara Angelina Galvão Magenta7, Hilda Maria Longhi Wagner8, Fábio de Barros9, Lúcia Garcez Lohmann5, Maria do Carmo Estanislau do Amaral2, Inês Cordeiro1, Sonia Aragaki1, Rosângela Simão Bianchini1 & Gerleni Lopes Esteves1 1Núcleo de Pesquisa Herbário do Estado, Instituto de Botânica, CP 68041, CEP 04045-972, São Paulo, SP, Brasil 2Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas – UNICAMP, CP 6109, CEP 13083-970, Campinas, SP, Brasil 3Programa Biota/FAPESP, Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas – UNICAMP, CP 6109, CEP 13083-970, Campinas, SP, Brasil 4Universidade Federal de São Carlos – UFSCar, Rod. João Leme dos Santos, Km 110, SP-264, Itinga, CEP 18052-780, Sorocaba, SP, Brasil 5Departamento de Botânica – IBUSP, Universidade de São Paulo – USP, Rua do Matão, 277, CEP 05508-090, Cidade Universitária, Butantã, São Paulo, SP, Brasil 6Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana – UEFS, Av. Transnordestina, s/n, Novo Horizonte, CEP 44036-900, Feira de Santana, BA, Brasil 7Universidade Santa Cecília – UNISANTA, R. Dr. Oswaldo Cruz, 266, Boqueirão, CEP 11045-907,
    [Show full text]
  • Poaceae: Pooideae) Based on Plastid and Nuclear DNA Sequences
    d i v e r s i t y , p h y l o g e n y , a n d e v o l u t i o n i n t h e monocotyledons e d i t e d b y s e b e r g , p e t e r s e n , b a r f o d & d a v i s a a r h u s u n i v e r s i t y p r e s s , d e n m a r k , 2 0 1 0 Phylogenetics of Stipeae (Poaceae: Pooideae) Based on Plastid and Nuclear DNA Sequences Konstantin Romaschenko,1 Paul M. Peterson,2 Robert J. Soreng,2 Núria Garcia-Jacas,3 and Alfonso Susanna3 1M. G. Kholodny Institute of Botany, Tereshchenkovska 2, 01601 Kiev, Ukraine 2Smithsonian Institution, Department of Botany MRC-166, National Museum of Natural History, P.O. Box 37012, Washington, District of Columbia 20013-7012 USA. 3Laboratory of Molecular Systematics, Botanic Institute of Barcelona (CSIC-ICUB), Pg. del Migdia, s.n., E08038 Barcelona, Spain Author for correspondence ([email protected]) Abstract—The Stipeae tribe is a group of 400−600 grass species of worldwide distribution that are currently placed in 21 genera. The ‘needlegrasses’ are char- acterized by having single-flowered spikelets and stout, terminally-awned lem- mas. We conducted a molecular phylogenetic study of the Stipeae (including all genera except Anemanthele) using a total of 94 species (nine species were used as outgroups) based on five plastid DNA regions (trnK-5’matK, matK, trnHGUG-psbA, trnL5’-trnF, and ndhF) and a single nuclear DNA region (ITS).
    [Show full text]
  • Poaceae) De México Species Richness, Classification and a Checklist of the Grasses (Poaceae) of Mexico
    Artículo de investigación Riqueza de especies, clasificación y listado de las gramíneas (Poaceae) de México Species richness, classification and a checklist of the grasses (Poaceae) of Mexico J. Gabriel Sánchez-Ken Resumen: Antecedentes y Objetivos: En 2006 se dio a conocer el catálogo de las gramíneas de México, pero desde entonces tanto la clasificación como la apa- rición de especies nuevas cambiaron las cifras de esta familia en México y en el mundo. El objetivo principal de este estudio fue actualizar la lista de taxa en México, reuniendo el mayor número posible de bases de datos en línea. Otros objetivos incluyeron la comparación de la riqueza de especies de la familia Poaceae entre los países con mayor diversidad, así como analizar la riqueza de sus taxa a nivel estatal y por categorías taxonómicas. Métodos: Se consultaron bases de datos en línea, tanto nacionales como extranjeras, que incluían ejemplares de referencia de México, se descarga- ron y se curaron, además de una confirmación visual de la identidad de un gran porcentaje de ejemplares. Se extrajeron los nombres de las especies, estados donde fueron colectadas y los acrónimos de los herbarios. La base resultante se complementó solo con bibliografía a partir de 2006 y la no incluida en el catálogo que mostraba ejemplares de respaldo. Resultados clave: Se revisaron 227,973 registros que contabilizaron, en total, 1416 taxa, que incluyen 215 géneros, 1312 especies y 214 categorías infraespecíficas. Del total de especies, 1092 se consideran nativas y 224 introducidas (cultivadas y/o malezas). Las especies endémicas de México son 304 (27.83%) o 311 (28.10%) si se consideran categorías inferiores.
    [Show full text]
  • TESIS Caracterización Ambiental De Los Ecosistemas, Zonas De Vida Y
    UNIVERSIDAD NACIONAL DANIEL ALCIDES CARRIÓN FACULTAD DE INGENIERÍA ESCUELA DE FORMACIÓN PROFESIONAL DE INGENIERÍA AMBIENTAL TESIS Caracterización Ambiental De Los Ecosistemas, Zonas De Vida Y Vegetación Natural De La Provincia De Pasco Para optar el título profesional de: Ingeniero Ambiental Autor: Bach. Yelitza Orializ VILLEGAS ALANIA Asesor: Ing. Anderson MARCELO MANRIQUE Cerro de Pasco – Perú – 2019 UNIVERSIDAD NACIONAL DANIEL ALCIDES CARRIÓN FACULTAD DE INGENIERÍA ESCUELA DE FORMACIÓN PROFESIONAL DE INGENIERÍA AMBIENTAL TESIS Caracterización Ambiental De Los Ecosistemas, Zonas De Vida Y Vegetación Natural De La Provincia De Pasco Sustentada y aprobada ante los miembros del jurado: Mg. Julio Antonio ASTO LIÑAN Mg. Luis Alberto PACHECO PEÑA PRESIDENTE MIEMBRO Mg. David Johnny CUYUBAMBA ZEVALLOS MIEMBRO 2 DEDICATORIA A mi abuelita, madre y hermanos por el apoyo constante, el empeño y la confianza que me dieron durante el desarrollo y culminación de mis estudios A mi alma mater por brindarme la oportunidad de llegar a ser una profesional 3 RECONOCIMIENTO A Dios por permitirnos tener una buena experiencia dentro de la universidad, por permitir convertimos en profesionales en lo que tanto nos apasiona A mi abuelita, madre y hermanos quienes me brindaron su valiosa y desinteresada orientación y guía en la elaboración del presente trabajo de investigación A cada docente que fue parte de este proceso integral de formación profesional, ya que también fueron un apoyo para poder llegar a esta etapa de nuestras vidas Y a todas las personas que en una u otra forma me apoyaron en la realización de este trabajo 4 RESUMEN La superficie de la Provincia de Pasco abarca dos Eco regiones de las 21 identificadas para nuestro país: La puna de los Andes Centrales y la jalca, las cuales involucran grandes paisajes naturales, con características de formaciones vegetales de matorrales y también de praderas abiertas de gramíneas en los “pajonales” altos andinos.
    [Show full text]
  • Revisión Taxonómica Del Género Molineriella Rouy (Gramineae: Poeae: Airinae) En La Península Ibérica
    Acta Botanica Malacitana1~P J.Cebrino Cruz & C. Romero-Zarco. Revisión taxonómica del género Molineriella Artículo203 '2,KWWSG[GRLRUJDEPYL&DEHFHUD $UWtFXOR Revisión taxonómica del género Molineriella Rouy (Gramineae: Poeae: Airinae) en la Península Ibérica Jesús Cebrino Cruz & Carlos Romero-Zarco 'HSDUWDPHQWR GH %LRORJtD 9HJHWDO \ (FRORJtD )DFXOWDG GH %LRORJtD 8QLYHUVLGDG GH 6HYLOOD & 3URIHVRU *DUFtD *RQ]iOH]VQ6HYLOOD(VSDxD Correspondencia Resumen C. Romero-Zarco e-mail: [email protected] Se presenta una revisión del género Molineriella Rouy sobre la base del Recibido: 11 septiembre 2017 estudio morfológico de 1.300 ejemplares de herbario. Se trata de un género Aceptado: 26 octubre 2017 PHGLWHUUiQHRTXHWLHQHVXPi[LPDULTXH]DHVSHFtÀFDHQOD3HQtQVXOD,EpULFD Publicado on-line: diciembre 2017 \SDUDHOTXHVHKDQUHFRQRFLGRDTXtWUHVHVSHFLHVSDUDODVFXDOHVVHDSRUWDQ descripciones y mapas de distribución en el área de estudio. Se han designado un neotipo para Aira laevis Brot. y sendos lectotipos para Periballia minuta subsp. australis3DXQHUR\Molineria minuta var. baetica Willk. Se ha descrito como nueva Molineriella laevis var. submutica Cebrino & Romero Zarco. Palabras clave: *UDPLQHDH3RHDH$LULQDH MolineriellaPeriballia3HQtQVXOD ,EpULFD7D[RQRPtD7LSLÀFDFLyQ Abstract Taxonomic revision of the genus Molineriella Rouy (Gramineae: Poeae: Airinae) in the Iberian Península We present the revision of the genus Molineriella Rouy based on a PRUSKRORJLFDOVWXG\RIKHUEDULXPVSHFLPHQV,WLVD0HGLWHUUDQHDQJHQXV ZLWKLWVPD[LPXPVSHFLHVULFKQHVVLQWKH,EHULDQ3HQLQVXOD7KUHHVSHFLHVDUH UHFRJQL]HG
    [Show full text]