Page S92 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS BIOINFORMATICS, 001 - 026 WP 012 SpecPlot – A Versatile Tool for Spectrum WP 001 Precursor Monoisotopic Mass and charge Visualization; Phil Bouchard; Nuno Bandeira; Center Determination with Almost 100% Accuracy; Xi Han1; for Computational Mass Spectrometry, UCSD, La Jolla, Baozhen Shan2; Bin Ma1; 1University of Waterloo, CA Waterloo, Canada; 2Bioinformatics Solutions Inc., WP 013 Improvement and Application of Analytical Software Waterloo, ON, Canada for Examining Reproducibility in Shotgun WP 002 Constructing a Minimal Spanning Tree for Proteomics; Xinjian Yan; Dmitrii V. Tchekhovskoi; Improving the Analysis of the Mass Spectrum of Bhaskar Godugu; Stephen Stein; NIST, Gaithersburg, Complex Mixtures; Yury Kostyukevich1, 2; Anton MD Grigoriev1, 2; 1, Dolgoprudnii, Russian Federation; WP 014 Fragment Selection and Scoring for Isomer Detection 2MIPT, Dolgoprudnii, by Tandem Mass Spectrometry; Javier Satulovsky; WP 003 A New Approach to Deisotoping of Complex Maggie Bynum; Hongfeng Yin; Kevin Killeen; Agilent Isotopically Resolved Spectra; Dmitry M. Avtonomov1, Laboratories, Santa Clara, CA 2; Ilya A Agron1, 3; Eugene Nikolaev1, 2; 1The Institute WP 015 Online Library of Peptide Fragmentation Mass for Energy Problems of Chemical Phys, Moscow, Spectra; Niksa Blonder; Qian Dong; Yuri Mirokhin; Jeri Russia; 2Institute of Biochemical Physics RAS, Moscow, Roth; Paul Rudnick; Stephen Stein; NIST, Gaithersburg, Russia; 3Institute of Biomedical Chemistry RAMS, MD Moscow, Russia WP 016 Creating Consensus Spectra with a Peak Density- WP 004 Deconvolution of Complex Tandem Mass Spectra of Based Clustering Method; Songfeng Wu; Bhaskar Intact Proteins: A Combinatorial Approach; Xiaowen Godugu; Xiaoyu Yang; Steve Stein; NIST, Gaithersburg, Liu1; Yuval Inbar1; Sara Weitz2; Pieter Dorrestein 2; MD Puneet Souda3; Julian Whitelegge3; Vineet Bafna1; Pavel WP 017 Evaluation of Methods for Unsupervised 1 1 Classification of MALDI Mass Spectra; Joseph Pevzner ; Dept. of Comp. Sci. and Eng., UCSD, La 1 1 1 Jolla, CA; 2Skaggs School, UCSD, La Jolla, CA; Salisbury ; Emmanuel Obasuyi ; Nicole Zaia ; Kristin 3 Boggio1; Nathalie Agar2; Jeffrey Agar1; 1Brandeis Pasarow Mass Spectrometry Laboratory, UCLA, Los 2 Angeles, CA University, Waltham, MA; Brigham and Women's WP 005 Accurate Measurement of Biopolymer Molecular Hospital, Harvard University, Boston, MA WP 018 Outlier Analysis of Protein Microarray Data for Weight with Orbitrap Mass Spectrometer; Zheming 1 Gu; Dawei Zhou; XenoBiotic Laboratories, Inc., Biomarker Discovery; Huy Vuong ; David M. Lubman 1; Michael S. Sabel1; Sara Forrester2; Timothy Barder2; Plainsboro, NJ 1 2 WP 006 Quantitative and Qualitative Aspects of University of Michigan, Ann Arbor, MI; Eprogen, Inc, Deconvolution of Intact Proteins; Natalia Belyaeva; Darien, IL Jim Shofstahl; Thomas Mcclure; Tonya Second; David WP 019 Automated Analysis of Hydrogen/Deuterium Horn; Thermo Fisher Scientific, San Jose, CA Exchange-Mass Spectrometry Data of Large Proteins WP 007 A Novel MS/MS Typing-Based Platform for to Determine Exchange Rate Constant with Near Automated Selection and Detection of Strain-Specific Single-Residue Resolution; Zhongqi Zhang; Gang Biomarkers.; Jane Razumovskaya; Appavu Sundaram; Xiao; Amgen, Thousand Oaks, CA Vladimir M. Doroshenko; SESI, Columbia, MD WP 020 Strategy for Automatic Interpretation of MS Data in WP 008 Exploring Data-Dependent Acquisition Strategies Target Compound Analysis: Evaluation Methods for with COM Instrument Control Libraries for the Comparing Data Against Acceptance Criteria; David LTQ-Orbitrap (Thermo Scientific) Instruments.; N. Heller; Hui Li; FDA/CVM, Laurel, MD Aleksey Nakorchevsky; John Yates; The Scripps WP 021 Biomarker Discovery by Automatic Annotation of N- Research Institute, La Jolla, CA glycan Species in MALDI-TOF-TOF Spectra; Chuan- WP 009 mzServer: Remote Mass-Informatics through a Web- Yih Yu; Anoop M. Mayampurath; Yehia Mechref; Based mzAPI; Manor Askenazi1; James Webber2; Haixu Tang; Indiana University, Bloomington, IN Saurav Singh2; Jarrod Marto2; 1Dana-Farber Cancer WP 022 Improving Wavelet Denoising of HPLC-MS Data by Institute and Hebrew University, Boston, MA; 2Dana Adaptive Thresholding in the Retention Time Domain; Salvatore Cappadona1, 2; Linda Pattini3; Farber Cancer Institute, Boston, MA 4 1, 2 WP 010 First Demonstration on NSE Biomarker of a Fredrik Levander ; Reinout Raijmakers ; Shabaz Mohammed1, 2; Albert J.R. Heck 1, 2; Bas van Breukelen1, Computational Environment Dedicated to Lab-on- 2 1 Chip Based Cancer Diagnosis; Pierre Grangeat1; ; Biomolecular MS & Proteomics - Utrecht University, 1 1 Utrecht, Netherlands; 2Netherlands Proteomics Centre, Laurent Gerfault ; Caroline Paulus ; Vangelis 3 Kritsotakis2; Manolis Tsiknakis2; Frédérique Lisacek3; Utrecht, Netherlands; Bioengineering Department - 4 5 Politecnico di Milano, Milano, Italy; Pierre-Alain Binz ; Manuel M Perez-Perez ; Mathieu 4 Trauchessec6; Virginie Brun6; 1CEA, LETI, MINATEC, Immunotechnology Dept, Lund University, Lund, DTBS, LE2S, Grenoble, France; 2FORTH, ICS, Sweden Heraklion, Greece; 3SIB, Proteome Informatics Group, WP 023 mMass Reloaded - Advances in the Popular Cross- 4 Platform Software for Precise Analysis Of Mass Geneva, Switzerland; GeneBio SA, Geneva, 1 1, Switzerland; 5ATOS Research and Innovation, Madrid, Spectrometric Data; Martin Strohalm ; Daniel Kavan 6 2; Petr Novak1, 2; Vladimir Havlicek1, 3; 1Institute of Spain; CEA/INSERM/UJF U880, LEDyP, Grenoble, 2 France Microbiology, Prague, Czech Republic; Faculty of Science, Charles University, Prague, Czech Republic; WP 011 Beyond Monoisotopic Mass II : Prediction of Peptide 3 PTMs by Isotope Cluster Analysis; Jonathan A Palacky University, Olomouc, Czech Republic Epstein1; Matthew T. Olson2; Peter S. Backlund1; Paul S. WP 024 Digging Deeper into a Million-Spectral Dataset: Blank1; Aaron Catlin1; Alfred L. Yergey 1; 1NICHD, Analysis of 3D-Fractionated Salivary Proteome by NIH, Bethesda, MD; 2JHMI, Baltimore, MD Using Search Algorithms for High-Mass-Accuracy 58th ASMS Conference on Mass Spectrometry Page S93 WEDNESDAY POSTERS Data.; Pratik Jagtap1; Sricharan Bandhakavi2; Sean L. Metz; Netherlands Vaccine Institute, Bilthoven, Seymour3; Tim Griffin2; 1Minnesota Supercomputing Netherlands Institute, UMN, Minneapolis, MN; 2University of WP 037 Determination of the Binding Sites of Minnesota, Minneapolis, MN; 3AB SCIEX, Foster City, Dimethylphenylisocyanate on Human Serum CA Albumin; Justin M. Hettick; Tinashe B. Ruwona; Paul WP 025 A Mathematical Algorithm for Estimation Protein D. Siegel; NIOSH, Morgantown, WV Dynamics Using Mass Spectrometry; Sergei Ilchenko WP 038 Identification of MDI Albumin Adducts with Liquid 2; Nadia Rachdaoui 2; Ling Li1; Belinda Willard1; Arthur Chromatography Tandem Mass Spectrometry and McCullough1; Takhar Kasumov1; 1CCF, Cleveland, OH; Mascot Software; Fagen Zhang; Michael J. Bartels; The 2Case Western Reserve University, Cleveland, OH Dow Chemical Company, Midland, MI WP 026 Probabilistic Scoring of Protein-Protein Interactions WP 039 Characterization of Challenging PEGylated from Label-Free Quantitative AP-MS Data; Therapeutics Utilizing Advanced MS Technology and Hyungwon Choi1; Ashton Breitkreutz2; Dattatreya Novel Preparation Techniques; Tonya Pekar Second1; Mellacheruvu1; Damian Fermin1; Mike Tyers2, 3; Rosa Viner1; Andrew Carr 2; Chad Cummins2; John E. P. Zhaohui Qin1; Anne-Claude Gingras2, 4; Alexey Syka1; Lihua Huang2; 1Thermo Fisher Scientific, San Nesvizhskii1; 1University of Michigan, Ann Arbor, MI; Jose, CA; 2Eli Lilly and Company, Indianapolis, IN 2Samuel Lunenfeld Research Institute, Toronto, Canada; WP 040 Effect of Fluorescent Dye Labeling on Electrospray 3University of Edinburgh, Edinburgh, UK; 4University of Ionization Efficiencies of Peptides and Proteins; Suraj Toronto, Toronto, Canada Saraswat; Dragan Isailovic; University of Toledo, PROTEINS: MODIFIED, 027 - 060 Toledo, OH WP 027 Identification of Acrolein Adducts by LC/MS/MS; WP 041 Formation of Carbamino Groups with Peptides and Maria Ospina; Alina Costin; Hubert Vesper; Proteins Studied by Mass Spectrometry; Donald J. Center/Disease Control & Prevention, Atlanta, GA Douglas; Peran Terrier; University of British Columbia, WP 028 Acetaldehyde Adducts as a Biomarker of Exposure; Vancouver, Canada Alina Costin; Maria Ospina; Hubert Vesper; Centers for WP 042 Use of Label-Free Quantitative Mass Spectrometry to Disease Control and Prevention, Atlanta, GA Identify Lyric Ubiquitylation Sites: Towards the WP 029 Characterization of Intact Complexes of Determination of Lyric Functional Modulation; Karin Barnouin1; Hayley Thirkettle2, 3; Sarah Hanrahan1; Nick Recombinant Albumin Domains with ON 01910, an 1 2 2 1 Antineoplastic Benzylstyryl Sulfone, Using Totty ; David Neal ; Hayley Whitaker ; London Electrospray Mass Spectrometry; John Roboz; Sool Research Institute, Cancer Research UK, London, UK; 2Cambridge Research Institute, Cancer Research UK, Yeon Cho; Mount Sinai School of Medicine, New York, 3 NY Cambridge, UK; Paterson Institute for Cancer WP 030 Interaction Site of Periplasmic Metal-Resistance Research, Manchester, UK Proteins CusF/CusB Determined by Chemical Cross- WP 043 Characterization of Trisulfide -- A Common Linking and Mass Spectrometry; Tiffany Mealman1; Modification in Antibodies; Sheng Gu; Dingyi Wen; Pragya Singh2; David R. Goodlett 2; Megan McEvoy1; R.Blake Pepinsky; Paul H. Weinreb; Yaping Sun; Lihe Vicki H. Wysocki1; 1University of Arizona, Tucson, AZ; Zhang; Susan Foley; Sha Mi; Werner Meier; Biogen 2University of Washington, Seattle, WA Idec, Cambridge, MA WP 031 Reporter Ion Signature for Cross-Linked Peptides; WP 044 MS Analysis of Differentially Acetylated Species of Lutz Fischer; Helena Barysz; Zhuo Chen; Lau Sennels; CheY, Response Regulator in Chemotaxis of E.coli. Salman Tahir; Juri Rappsilber; Wellcome Trust Centre From Top-Down to Bottom-Up; Tevie Mehlman; for Cell Biology, Edinburgh, UK Bassem Ziadeh; Orna Liarzi; Rina Barak; Michael WP 032 Structural Characterization of Chemically Stabilized, Eisenbach; Alla Shainskaya; Weizmann Institute of ALS-Associated Variants of Cu, Zn Superoxide Science, Rehovot, Israel Dismutase.; Jared R. Auclair; Kristin J. Boggio; Dagmar WP 045 Mass Spectrometric Identification of Human CENP- A Post-Translational Modifications; Aaron Bailey1; Ringe; Gregory A. Petsko; Jeffrey Agar; Brandeis 2 2 1 University, Waltham, MA Tatyana Panchenko ; Ben Black ; Daniel Foltz ; Jeffrey Shabanowitz1; Donald Hunt1; 1University of Virginia, WP 033 Cysteine Rich Protein Segments: A Comparison of 2 Disulfide Mapping Methods; Cedric Bobst1; Adriana Z. Charlottesville, VA; University of Pennsylvania, Kita1; Igor A. Kaltashov1; Melanie Lin2; Paul Salinas2; Philadelphia, PA John J. Thomas2; Philip J. Savickas2; 1University of WP 046 Role of Methionine Sulfoxide Reductase in Repairing 2 Catalase of Helicobacter pylori; ViLinh Tran1; Manish Massachusetts, Amherst, MA; Shire HGT, Cambridge, 2 2 1 1 MA Mahawar ; Robert J. Maier ; Joshua S. Sharp ; Complex Carbohydrate Research Center, UGA, Athens, GA; WP 034 Disulfide Mapping of Therapeutic Proteins by 2 Differential Mass Spectrometry (dMS); Yi Du; Van Department of Microbiology, UGA, Athens, GA WP 047 Identification of H-Ras Posttranslational M. Hoang; Merck Research Laboratories, West Point, 1 PA Modifications under Oxidative Stresses; Yuhuan Ji ; Dagmar J. F. Haeussler2; Markus M. Bachschmid2; WP 035 Insights into Formaldehyde Cross-Linking of 1 2 1 Proteins and Cross-Link Reversal by Mass David Perlman ; Richard A. Cohen ; Cheng Lin ; 1Department of Biochemistry, Boston University, Boston, Spectrometry Based Time-Course Analysis; Xuan 2 Ding; Juergen Kast; University of British Columbia, MA; Department of Medicine, Boston University, Vancouver, BC Boston, MA WP 036 BPL Modifications in Viruses: Reactions with Model WP 048 Mapping Free Radical Initiated Oxidation of apo B- 100 Low Density Lipoprotein by LC-MS/MS; Sourav Peptides.; Joost Uittenbogaard; Bert Zomer; Peter 1 2 1 Hoogerhout; Hugo D. Meiring; Ad de Jong; Bernard Sourav Chakraborty ; Yang Cai ; Matthew A. Tarr ; 1Department of Chemistry, University of New Orleans, Page S94 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS New Orleans, LA; 2The Research Institute for Children, Keppel; David Weis; University of Kansas, Lawrence, New Orleans, LA KS WP 049 Semi-Quantitative Analysis of Combinatorial Post- WP 062 Structure/Dynamics Studies of Dodecameric DPS- Translational Modifications on Full-Length Histone like Cages from Bacteria and Archaea (Mesophilic H4 of Cow, Mouse and Chicken; Li Zhou 1; Michael vs. Thermophilic); Navid Movahed1; Geoff Blatter1; Parra 1; Yuan-Yu Lee1; Yanbao Yu1; Yi Huang2; Brian Jonathan Hilmer2; George Gauss1; Martin Lawrence1; D. Strahl 1; Xian Chen1; 1University of North Carolina - Mark Young1; Trevor Douglas1; Brian Bothner1; Chapel Hill, Chapel Hill, NC; 2Fudan University, 1Montana State University, Bozeman, MT; 2Montana Shanghai, China State University, Mass Spectrometry Facili, Bozeman, WP 050 Localization and Quantification of Free Sulfhydryl in MT Monoclonal Antibodies by Top-Down HPLC/MS WP 063 Histidine Hydrogen-Deuterium Exchange Mass Analysis; Pavel V. Bondarenko; Gang Xiao; Thomas M. Spectrometry for Probing Structural Changes in Dillon; Zhongqi Zhang; Amgen, Inc., Thousand Oaks, Rhodopsin; Masaru Miyagi; David Lodowski; CA Krzysztof Palczewski; Case Western Reserve Univ., WP 051 Fourier Transform Mass Spectrometry of Cleveland, OH Antiproliferative Agents and Their Protein Targets; WP 064 Understanding the Transferrin Binding Protein B – Jeremy J. Wolff1; DongEun Lee2; Matthew L. Meketa2; Porcine Transferrin Interaction and its Role in Iron Andrew G. Myers2; 1Bruker Daltonics, Billerica, MA; Release and Transfer; Leslie Silva; Ronghua Yu; Tony 2Harvard University, Cambridge, MA B. Schryvers; David C. Schriemer; University of WP 052 Discovery of New Protein Methyltransferases and Calgary, Calgary, Canada Relative Quantitation of the Methylation State of WP 065 An Integrated Microfluidic Chip for Spatially Their Translational Machinery Substrates Using Resolved Measurements of Millisecond Time-Scale Mass Spectrometry; Kristofor Webb; Rebecca Lipson; Conformational Dynamics in Proteins.; Tamanna Rob; Julian Whitelegge; Steve Clarke; University of Derek Wilson; York University, Toronto, Canada California LA, Los Angeles, CA WP 066 Understanding Oncogenic Activation and Inhibitor WP 053 Analysis of Fluorescently Labeled Proteins by Binding of the Epidermal Growth Factor Receptor MALDI-MS; Dragan Isailovic; Suraj Saraswat; Nidhi by Hydrogen Exchange Mass Spectrometry; Kasper Jaiswal; University of Toledo, Toledo, OH D. Rand1; Cai-hung Yun2, 3; Michael J. Eck2, 3; John R. WP 054 Top-Down Sequencing of Intact Histones by MALDI- Engen1; 1Northeastern University, Boston, MA; TOF In Source Decay; Elaine Stephens; Sew Peak 2Harvard Medical School, Boston, MA; 3Dana-Farber Chew; Medical Research Council, Cambridge, UK Cancer Institute, Boston, MA WP 055 Top-Down ECD Analysis of Beta 2 Microglobulin WP 067 Investigation of the Protein Dynamics of AMP- Protein Deamidation; Xiaojuan Li 1; Cheng Lin 1; Peter Activated Protein Kinase with Hydrogen/Deuterium B. O'connor2; 1Boston University School of Medicine, Exchange Mass Spectrometry; Rachelle R. Landgraf1; Boston, MA; 2University of Warwick, Coventry, UK Michael J. Chalmers1; Bruce D. Pascal1; Francis WP 056 Identification of Different Protein Isoforms in C. Rajamohan2; Melissa S. Harris2; Ravi G. Kurumbail2; elegans Using the Molecular Weight Fractionation Patrick R. Griffin1; 1The Scripps Research Institute, and Shotgun Proteomics; Gennifer Merrihew; Sanna Jupiter, FL; 2Pfizer Inc., Groton, CT Oddone; Michael J. Maccoss; University of Washington, WP 068 HDX Reveals DNA Binding Modulates Ligand- Seattle, WA Dependent Co-Activator Interaction with the WP 057 HDAC3 and HDAC8 Alkylation by a Selective VDR/RXRĮ Nuclear Receptor Complex; Jun Zhang 1; Photoaffinity Labeling Inhibitor; Yongsoo Choi 1; Bai Michael J. Chalmers1; Bruce D. Pascal1; Scooter Willis1; He1; Raghupathi Neelarapu1; Subash Velaparthi2; Sylvie Keith R. Stayrook2; Jeffrey A. Dodge2; Patrick R. Blond1; Pavel A Petukhov1; Richard B van Breemen1; Griffin1; 1The Scripps Research Institute, Scripps 1University of Illinois College of Pharmacy, Chicago, Florida, Jupiter, FL; 2Lilly Research Laboratories, Eli IL; 2The Scripps Research Institute, La Jolla, CA Lilly and Company, Indianapolis, IN WP 058 Localization of the Specific Site of Enzyme-Mediated WP 069 Screening for Unique RXRĮ-rexinoid Interactions by Covalent Binding of Phycoerythrobilin to C- Automated Hydrogen/Deuterium Exchange; Leeann Phycoerythrin; M. Nazim Boutaghou; Avijit Biswas; Boerma; Gang Xia; Donald Muccio; Matthew B. Richard B. Cole; Wendy M. Schluchter; University of Renfrow; University of Alabama at Birmingham, New Orleans, New Orleans, LA Birmingham, Al WP 059 Use of Hybrid LTQ-Orbitrap to Detect Ubiquitin and WP 070 Cross Comparison of Differential HDX MS Datasets; Polyubiquitin-Chain-Derived Peptides in Unenriched Bruce D. Pascal1; Michael J. Chalmers1; Scooter Willis1; Whole-Cell and Tissue Lysates; Thomas A. Shaler1; Jeffrey A. Dodge2; Stephen J. Iturria2; Jun Zhang1; Brigit Riley2; Steve Kaiser2; Ron Kopito2; Chris Patrick R. Griffin1; 1The Scripps Research Institute, Becker1; 1Caprion Proteomics U.S. LLC, Menlo Park, Scripps Florida, Jupiter, FL; 2Eli Lilly and Company, CA; 2Stanford University, Stanford, CA Indianapolis, Indiana WP 060 Mapping the Chromatin Structure Controlling Life WP 071 Allostery and Feedback Regulation in the Cyclic Cycle Progression of Plasmodium Falciparum; Anita AMP Pathway Unravelled by Amide Saraf2; Serena Cervantes1; Michael Washburn2; Karine Hydogen/Deuterium Exchange Mass Spectrometry; Le Roch1; Laurence Florens2; 1University of California Ganesh S. Anand; Balakrishnan S. Moorthy; Suguna Riverside, Riverside, CA; 2Stowers Institute for Medical Badireddy; NUS, Singapore Research, Kansas City, MO WP 072 Development of an HXMS Workflow and its PROTEIN CONFORMATION: (HDX), 061 - 088 Application to Epitope Mapping; Keith Fadgen; WP 061 Localizing Binding-Induced Folding in Disordered Michael Eggertson; Martha Stapels; Joomi Ahn; Waters Proteins CBP and ACTR with H/D-MS; Theodore Corp., Milford, MA 58th ASMS Conference on Mass Spectrometry Page S95 WEDNESDAY POSTERS WP 073 Combination of Top-Down and Bottom-Up University, Boston, MA; 2Waters Corporation, Milford, Approaches to HDX MS: On-Line Digestion and MA Continuous Flow Injection Followed by Peptide Ion WP 085 Probing the HIV-1 Vif:Cullin 5 Interaction Using ECD/ETD; Rinat Abzalimov1; Cedric Bobst1; Michael Hydrogen Exchange Mass Spectrometry; Sean R. Easterling2; Igor A. Kaltashov1; 1University of Marcsisin; John R. Engen; Northeastern University, Massachusetts, Amherst, MA; 2Bruker Daltonics, Inc., Boston, MA Billerica, MA WP 086 Conformational Changes of an Integral Membrane WP 074 Interactions of Various Modulators with Human Protein Studied by Hydrogen/Deuterium Exchange FXR-LBD Studied by Hydrogen/Deuterium Mass Spectrometry; Yan Pan1; Leonid Brown2; Lars Exchange with LC-ESI Mass Spectrometry; Liping Konermann1; 1University of Western Ontario, London, Yang1; Claudia Maier1; 1Oregon State University, Canada; 2University of Guelph, Guelph, Canada Corvallis, OR WP 087 Membrane-Assisted Hydrogen/Deuterium Exchange WP 075 Investigating Novel Molecular Events Involved in Mass Spectrometry for Protein Dynamics and Calcium-Calmodulin Activation of Smooth Muscle Protein/Protein Interaction Studies; Juan Astorga- Myosin Light Chain Kinase Using Hydrogen- Wells; Michael Fitzen; Hans Jörnvall; Karolinska Deuterium Exchange Mass Spectrometry; Madonna Institutet, Stockholm, Sweden Lily Choi1; Michael Walsh1; John Chik1, 2; 1University of WP 088 Mapping the Interfaces of Insulin Oligomers by H/D Calgary, Calgary, Canada; 2Mount Royal University, Amide Exchange and Top-Down Mass Spectrometry; Calgary, AB Yining Huang1; Weidong Cui 2; Michael L. Gross2; WP 076 The Transport Cycle of a Membrane ABC 1WUSTL, St. Louis, MO; 2Washington University, St. Transporter Probed by HDX MS.; Shahid Mehmood; Louis, MO Jean-Michel Jault; Eric Forest; Inst. for Structural PROTEOMICS TISSUE, 089 - 106 Biology, Grenoble, France WP 089 Dysregulation of PSD and NMDA Receptor Complex WP 077 Measurement of pKa Values and C2 H/D Exchange Composition and Activity in Schizophrenia; Matthew Rates of Individual Histidine Residues in Proteins L Macdonald1; Eugene F. Ciccimaro2; Anamika Using MALDI-MS; Naoka Hayashi1; Hiroki Kuyama2; 1 1 3 1 2 2 Banerjee ; Chang-Gyu Hahn ; Ian A. Blair ; University Chihiro Nakajima ; Osamu Nishimura ; Masaru of Pennsylvania, Philadelphia, PA; 2ThermoFisher Miyagi3; Hisayuki Matsuo4; Takashi Nakazawa1; 1Nara 3 2 Scientific, Somerset, NJ; Univ. of Women's University, Nara, Japan; Institute for Protein Penn/SOM/Pharmacol, Philadelphia, PA Research, Osaka, Japan; 3Case Western Reserve 4 WP 090 Unlocking the Proteome of Formalin-Fixed CNS University, Cleveland, Ohio; National Cardiovascular Tissue: Optimisation and Application to the Study of Center Research Institute, Osaka, Japan Multiple Sclerosis; Linda Ly1; Ben Crossett2; John W. WP 078 Using Hydrogen/Deuterium Exchange Mass Prineas1; Michael Barnett1, 3; 1Central Clinical School, Spectrometry to Study the Conformation of University of Sydney, Australia; 2School of Molecular Myoglobin Adsorbed on Silver Nanoparticles; and Microbial Biosciences, University of Sydney, Yaoling Long; Barber David; David Powell; John Eyler; Australia; 3Brain and Mind Research Institute, University of Florida, Gainesville, FL University of Sydney, Australia WP 079 The Potential for "Synergistic" Protein Digestion WP 091 Chamber-Related Variation in Phosphorylation State Using Multiple, Immobilized Protease for of Cardiac Troponin Revealed by High Resolution Hydrogen/Deuterium Exchange Mass Spectrometry; Top-Down Mass Spectrometry; Xintong Dong; Jiang Anne Yang2; John Chik1, 2; 1Mount Royal University, 2 Zhang; Timothy Hacker; Holly Norman; Jitandrakumar Calgary, Canada; University of Calgary, Calgary, AB Patel; Takushi Kohmoto; Richard Moss; Ying Ge; WP 080 Slow H/D Exchange of Histidine Residues as a Probe University of Wisconsin-Madison, Madison, WI of Protein Folding and Stability; Duc Tran; Michael C. WP 092 Differential Proteomics Analysis in Ischemic Cardiac Fitzgerald; Duke University, Durham, North Carolina Disease; Evelyn H. Kim1; Vladimir I. Galchev1; WP 081 Recognizing False EX1 Signatures Caused by Peptide Sharlene M. Day1; Francis Pagani1; JinYoung Kim2; Carryover during HXMS Analyses; Jing Fang; Kasper Yangsun Kim3; David E. Misek1; Margaret V. Westfall1; D. Rand; Penny J. Beuning; John R. Engen; 1University of Michigan, Ann Arbor, MI; 2Korea Basic Northeastern University, Boston, MA Science Institute, Ochang, South Korea; 3Hudson WP 082 Apolipoprotein-E Oligomerization Investigated by Surface Technology, Newark, NJ Hydrogen/Deuterium Exchange Mass Spectrometry; WP 093 Proteomic Analysis of Formalin-Fixed Paraffin- Richard Yu-Cheng Huang; Brian C. Gau; Kanchan Embedded (FFPE) Her2 Positive Breast Cancer Garai; Carl Frieden; Michael L. Gross; Washington Tissue Using Differential Mass Spectrometry; University, St.Louis, MO Ekaterina G. Deyanova; Qinghua Song; Nathan Yates; WP 083 Front-End Automation for Solution-Phase H/D Ronald Hendrickson; Merck & Co. Inc., Rahway, NJ Exchange FT-ICR Mass Spectrometry; Qian Zhang1, 2 1 1 1 WP 094 Global Proteomics of Early-Stage Recurrent Breast ; Greg T. Blakney ; Mark R. Emmett ; Huimin Zhang ; Cancer; Nicholas Bateman1; Jenny Heidbrink B. Kerry Maddox3; Scott M. Stagg2, 3; Alan G. Marshall1, 2 2 1 2 1 Thompson ; Marlene M. Darfler ; Mai Sun ; Brian L ; Ion Cyclotron Resonance Program, NHMFL, Hood1; Sheeno Thyparambil2; Todd Hembrough2; Tallahassee, Florida; 2Department of Chemistry and 1 2 1 3 Thomas P. Conrads ; David Krizman ; University of Biochemistry, FSU, Tallahassee, Florida; Institute of Pittsburgh Cancer Institute, Pittsburgh, PA; 2Expression Molecular Biophysics, FSU, Tallahassee, Florida Pathology Inc., Rockville, MD WP 084 Investigating Conformational Changes in Oxidized WP 095 Handling of Clinical Samples for Improved Interferon alpha-2b by Hydrogen/Deuterium Proteomics; John Lindsay1; Marcus Svensson1; Mats Exchange Mass Spectrometry (HX MS); Joomi Ahn1; 1 2 3 1 2 1 1 Borén ; Per Svenningsson ; Per Andrén ; Karl Sköld ; Keith Fadgen ; John R. Engen ; Northeastern 1Denator AB, Gothenburg, Sweden; 2Inst. for Physiology Page S96 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS and Pharmacology, Karolinska Institute, Stockholm, Esbenshade; Diana Donnelly-Roberts; Lance Lee; Shaun Sweden; 3Biological and Medical Mass Spectrometry, Mcloughlin; Abbott Labs, Abbott Park, IL Uppsala University, Uppsala, Sweden PROTEOMICS: PLASMA, 107 - 117 WP 096 Label-Free LC-MS-Based Proteomics for Integrated WP 107 A Combined Proteomic and Lipidomic Approach for Preclinical Pharmaceutical Toxicology: Experience Biomarker Discovery of Obstetric Disorders in from the FP6 InnoMed PredTox Consortium; Ben Individual Patient Plasma during the First Collins1; Christine Miller2; Martin Wells3; William 1 1 1 1 1 Trimester; Susan E. Slade ; Eamonn Breslin ; John P. Gallagher ; Stephen Pennington ; UCD Conway Shockcor2; Hayley Crowe2; Stephen Mcdonald2; Ranjit Institute, University College Dublin, Dublin, Ireland; 3 3 1 2 3 Akolekar ; Kypros Nicolaides ; Steven Thornton ; James Agilent Technologies, Santa Clara, CA; Nonlinear Scrivens1; 1University of Warwick, Coventry, UK; Dynamics, Newcastle upon Tyne, UK 2Waters Corp, Milford, MA; 3Kings College Hospital, WP 097 A Quantitative Proteomic Study on the Inhalable London, UK Nanoparticle-Induced Lung Injury Animal Model; 1 2 2 WP 108 A Comprehensive Analysis of Swine Plasma Mei Ling Wu ; Chia-Hua Chen ; Jui-Ping Li ; Yi-Ting Proteome Using Combinatorial Peptide Ligand Wu2; Jen-Kun Chen2; Chung-Shi Yang2; Yi-Ting Chen1; 1 2 Libraries (CPLL), Dual-Enzyme Digestion and Dual- Chang Gung University, Taoyuan, Taiwan; National Activation Sequencing with LTQ/Orbitrap/ETD; Jun Health Research Institutes, Miaoli, Taiwan Li; Marc S. Halfon; Jun Qu; University at Buffalo, WP 098 Quantitative Proteomic Analysis of Gill in Zebrafish Buffalo, NY (Danio rerio) Exposed to Naphthenic Acids from Oils WP 109 Deep Quantitative Profiling Reveals a Substantial Sands Process Water; Andrea De Souza; Tyson Effect of Post Menopausal Hormone Therapy on the MacCormack; Greg Goss; Liang Li; University of Serum Proteome; Sharon Pitteri1; Aaron Aragaki1; Alberta, Edmonton, Canada Hiroyuki Katayama1, 3; Sophie Paczesny1, 2; Lynn WP 099 Quantitative Analysis of the brain Synaptic Plasma Amon1; Hong Wang1; Qing Zhang1; Tina Busald Membrane Proteins from the DHA-Deficient and Buson1; Melissa Johnson1; Lin Chen1; Pei Wang1; Adequate Mice Using 16O/ 18O Labeling; Vishaldeep Martin Mcintosh1; Ross Prentice1; Samir Hanash1; 1Fred Sidhu; Bill Huang; Hee-Yong Kim; NIAAA/NIH, Hutchinson Cancer Research Center, Seattle, WA; Rockville, MD 2University of Michigan, Ann Arbor, MI; 3Eisai Inc., WP 100 Effect of Botanicals on Regulation of Insulin Action Andover, MA in Primary Human Skeletal Muscle Culture Using WP 110 Optimizing Plasma Digestion for Quantitative Mass Spectrometry; Peter Scherp; Indu Kheterpal; Ku Proteomic Studies: Diagnostic Peptides as Ginger; Zhong Wang; William Cefalu; Pennington Endogenous Markers of Incomplete Digestion; Naomi Biomedical Research Center, Baton Rouge, LA R. Choodnovskiy1; Margaret Pyle1; Susan E. WP 101 Broad-Scale Proteomic Analysis from Less Than 3ug Abbatiello1; Leigh Anderson2; Derek Smith3; Steven A. Protein Derived from Human Prostate Epithelium Carr1; 1Broad Institute, Cambridge, MA; 2Plasma Isolated Using Laser Capture Microdissection; Kristin 3 1 1 2 2 Proteome Institute, Washington, DC; UVic-Genome BC West ; Nikhil Garge ; Zhi Huang ; Andra Frost ; Proteomics, Victoria, BC Maureen Bunger1; 1Research Triangle Institute, 2 WP 111 Plasma Proteome Profiling of HIV-1/HCV Mono and Research Triangle Park, NC; University of Alabama at Co-Infected Individuals by Multiplex iTRAQ Birmingham, Birmingham, AL Quantitative Proteomics; Vivekananda Shetty1; WP 102 Immune-Related Changes in Oxidative Stress of Sinnathamby Gomathinayagam1; Zacharie Nickens 1; SAMP8 Mice following in vivo N-acetylcysteine Edward Acheampong2; Pooja Jain2; Ramila Philip1; Treatment: A Proteomics Assessment; Renã A. S. 1 2 1 3 2 Immunotope, Inc., Doylestown, PA; Drexel Inst of Robinson ; D. Allan Butterfield ; William A. Banks ; Biotechnology and Virology Research, Doylestown, PA William M. Pierce4; 1University of Pittsburgh, 2 WP 112 Enhancement in MALDI Profiling of the Low Pittsburgh, PA; Saint Louis University, Saint Louis, Molecular Weight Human Serum Proteome Using MO; 3University of Kentucky, Lexington, KY; 1 2 4 Ultrafiltration; Christine Bunai ; Krista White ; University of Louisville, Louisville, KY Christopher Wilkins2; Richard R Drake2; Maureen B. WP 103 2-DE-MALDI-TOF Tandem MS for Evaluation of Tracy 1; Dariya Malyarenko1; 1College of William and Protein Expression Patterns in Tissues of the Model Mary, Williamsburg, VA; 2Eastern Virginia Medical Fish Species, Fundulus grandis; Naga Abbaraju; M. School, Norfolk, VA Nazim Boutaghou; Richard B. Cole; Bernard B. Rees; WP 113 Combining Abundant Plasma Protein Depletion and University of New Orleans, New Orleans, LA the ALiPHAT Method for Quantitative Global and WP 104 Rat Muscle Proteome and Transcriptome: Targeted Proteomic Studies; Christopher M. Shuford 1; Differential Analysis According to Changes in Fuel Adam M. Hawkridge 1; John C. Burnett Jr. 2; David C. Partitionning; Thierry Wasselin; Chrystel Husser; Alain Muddiman1; 1North Carolina State University, Raleigh, van Dorsselaer; Fabrice Bertile; LSMBO, IPHC-DSA, NC; 2Mayo Clinic College of Medicine, Rochester, MN Strasbourg, France WP 114 Identification and Quantification of Plasma WP 105 Proteomic Comparison of the Peritoneal Dialysates in Biomarkers for Metabolic Syndrome in Ossabaw Chronic Diabetic and Glomerulonephritic Patients; 1 1 2 2 1 Swine; Jacob A. Galan ; Hui-Jie Zhang ; Lianshui Hsin-Chieh Wu ; Hung Su ; Ming-Hui Yang ; Yu- Wang2; Haixu Tang2; Michael Sturek3; Weiguo Andy Chang Tyan2; 1National Sun Yat-Sen University, 1 1 2 2 Tao ; Purdue University, West Lafayette, IN; Indiana Kaohsiung, Taiwan; Kaohsiung Medical University, University, Bloomington, IN; 3Indiana University School Kaohsiung, Taiwan of Medicine, Indianapolis, IN WP 106 Exploration of Resveratrol’s Target Profile Using WP 115 Sickle Cell Disease-Associated Alterations in Human Chemical Proteomics; Mary Shannon Duggan; Hua Plasma; David H. Perlman; Wantao Ying; Lei Li; Tang; Paul L. Richardson; Violeta Marin; Timothy Marianna Budnik; Vivek N. Bhatia; Lynda M. Reid; 58th ASMS Conference on Mass Spectrometry Page S97 WEDNESDAY POSTERS Elizabeth S. Klings; Martin H. Steinberg; Catherine E. WP 126 Changes in Protein Phosphorylation and Abundance Costello; Mark E. Mccomb; Boston University School of in a Drought Sensitive Rice MAP Kinase Mutant Medicine, Boston, MA Using Gel- and Liquid-Based Proteomics Approach.; WP 116 Characterization of Secreted Human Complement Sophie Alvarez1; Ellen Marsh1; Jing Ning2; Lizhong Component C3 in Breast Cancer to Reveal the Xiong2; Daniel Schachtman1; Leslie M. Hicks1; Related Enzyme Activity and Pathway; Zhi Zeng; 1Danforth Center, St Louis, MO; 2Huazhong Shiaw-Lin Wu; William Hancock; Barnett Institute, Agricultural University, Wuhan, China Northeastern University, Boston, MA WP 127 Identification of Kinases and Phosphorylation Sites WP 117 A Reproducible and Sustainable Two Dimensional Involved in MicroRNA Biogenesis; Erica Jacobs; Brian HPLC Method that Removes Highly and Moderately Chait; Rockefeller University, New York, NY Abundant Plasma Proteins Stepwise and WP 128 Characterization of Csn5/Jab1 Phosphorylation by Continuously; Weixun Wang; Jun Man; Nathan Yates; the IKK Complex and Its Biological Roles in TNF-Į Ronald Hendrickson; Merck Research Labs, Rahway, NJ Induced Signaling Pathway; Lei Fang; Lan Huang; PROTEOMICS: PHOSPHORYLATION PATHWAYS, University of California, Irvine, CA 118 - 139 WP 129 Study of T-cell Receptor (TCR) Complex Signaling Using Proteomic Approach; Wei-Yun Chen1; Meng- WP 118 Kinase Activity Assay for Kinome profiling 1 2 1 (KAYAK): A Versatile Multiplexed Mass Chieh Chen ; Mei-Chun Tseng ; Wen-Chin Yang ; Yet- Ran Chen1; 1Agricultural Biotechnology Research Spectrometry Based Assay for Measuring Kinase 2 Activity; Fiona E Mcallister1, 2; Ryan Kunz1; Kazuishi Center, Academia Sinica, Taipei, Taiwan; Institute of Kubota1; Mat Sowa1; Yonghao Yu1; Ramin Rad1; Chemistry, Academia Sinica, Taipei, Taiwan Wilhelm Haas1; Steven Gygi1; 1Harvard Medical WP 130 Elucidating ROCK Signaling Network through School, Boston, MA; 2Chemistry Department, Edinburgh Quantitative Phosphoproteomics Using 8-plex University, Edinburgh, Scotland Isobaric Tags, High Resolution Tandem Mass WP 119 Comparative Phosphoproteomics of the Nematode Spectrometry, and Alternative Fragmentation Models P. pacificus and C. elegans; Nadine Borchert1; Technologies.; Qiangwei Xia; Joshua J. Coon; Guokai Karsten Krug2; Silke Wahl2; Ralf J. Sommer1; Boris Chen; James Thomson; Univ of Wisconsin-Madison, Macek2; 1Max-Planck-Institute for Developmental Madison, WI Biology, Tübingen, Germany; 2Proteome Center, WP 131 Fyn Promotes Phosphorylation of Collapsin Response Mediator Protein 1 at Tyrosine 504, a Novel, Tübingen, Germany 1 WP 120 The Myocardial Phosphoproteome; Alistair V. G. Isoform-Specific Regulatory Site; Gwen Buel ; John 1 1 1 Rush2; Bryan Ballif1; 1University of Vermont, Edwards ; Melanie Y. White ; Benjamin L. Parker ; 2 Melanie Schulz2; Kasper Engholm-Keller2; Brett D. Burlington, VT; Cell Signaling Technology, Danvers, Hambly1; Martin R. Larsen2; Stuart J. Cordwell1; 1The MA University of Sydney, Sydney, Australia; 2Univ. Southern WP 132 Nucleotides and Growth Factors Modulate EGFR Denmark, Odense, Denmark Activation Distinctively; Amanuel Kehasse; David H. WP 121 Phosphorylation Detection by MudPIT Separation.; Perlman; Giuseppe Infusini; Mark E. McComb; Vickery Daniela Mavrici1; Andrew Schumacher2; Stuart C. Trinkaus-Randall; Catherine E. Costello; Boston Feinstein2; Lori Kohlstaedt1; 1QB3, University of University School of Medicine, Boston, MA California at Berkeley, Berkeley, CA; 2Neuroscience WP 133 Phosphoproteomics Analysis in Human Metastasis- Research Institute, UC Santa Barbara, Santa Barbara, Related Gene-Manipulated Breast Cancer Cells; Xiaolei Xie 1; Jintang He 4; Yashu Liu 1; Na Yang 5; Fan CA 2 3 1 WP 122 Phosphoproteomic Analyses of Phosphorylation Sites Xiang ; Steven Goodison ; David M. Lubman ; 1University of Michigan, Ann Arbor, MI; 2Shimadzu in Membrane Fractions of 400 mM High-Salt- 3 Stressed Arabidopsis Thaliana; Ing-Feng Chang1; Shu- Biotech, Pleasanton, CA; M. D. Anderson Cancer 1 2 3 1 Center - Orlando, Orlando, FL; 4Department of Surgery, Ying Wang ; Lan-Yu Wang ; Jue-Liang Hsu ; National 5 Taiwan U, Taipei, Taiwan; 2Mass Solutions Technology University of Michigan, Ann Arbor, MI; Universiyt of Co. Ltd., Taipei, Taiwan; 3National Pingtung University, Michigan, Medical Center, Ann Arbor, MI Pingtung, Taiwan WP 134 Phosphoproteomics Analysis of Leishmania mexicana WP 123 Quantitative Synthesis/Degradation Proteomic Major Life Stages, Wild Type and Kinase Deletion Analysis of Vps4B-Mediated Perturbations in EGFR Mutants by Multistage Activation LC-MS/MS; Heidi Rosenqvist1, 2; Martin Wiese1; Ole N. Jensen2; 1SIPBS, Signaling in Breast Cancer Cells under Hypoxic 2 Conditions; Zhongping Liao1; Yunhu Wan 1; Stefani University of Strathclyde, Glasgow, UK; Univ. of Thomas1; David Ann2; Austin Yang1; 1Univ of Maryland Southern Denmark, Odense, Denmark at Baltimore, Baltimore, MD; 2City of Hope, Duarte, CA WP 135 Study of Prostate-Specific G Protein-Coupled WP 124 Phosphoproteomic Analysis of Human Embryonic Receptor Signalling in Human LNCaP Cells by 1 3 Quantitative Phosphoproteomics: From Method Stem Cells; Laurence M. Brill ; Wen Xiong ; Ki-Bum 1 Lee2; Scott Ficarro4; Andrew Crain1; Yue Xu3; Alexey Evaluation to Biological Data.; Heike Piechura ; Katja 1 1 3 1 Kuhlmann1; Silke Oeljeklaus1; Martin Eisenacher1; Terskikh ; Evan Snyder ; Sheng Ding ; Burnham Instit 1 1 2 2 Christian Stephan ; Helmut E. Meyer ; Hanns H. Hatt ; for Med Res, La Jolla, CA; Rutgers University, 3 1 1 Piscataway, NJ; 3The Scripps Research Institute, La Eva M. Neuhaus ; Bettina Warscheid ; Ruhr- Jolla, CA; 4Dana-Farber Cancer Instit, Boston, MA Universität, Medizinisches Proteom-Center, Bochum, Germany; 2Ruhr-Universität, Department for WP 125 Proteome and Phosphoproteome Revealed Unique 3 Features of Mouse Embryonic Stem Cells; Qing-Run Cellphysiology, Bochum, Germany; Charite, Li; Xiao-Bin Xing; Tao-Tao Chen; Rong Zeng; Neurowissenschaftliches Forschungszentrum, , Shanghai Institutes for Biological Sciences, CAS, Germany Shanghai, China Page S98 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS WP 136 Mode of Action Analysis of Sorafenib by Integrating Pascovici; Brian Atwell; Paul A. Haynes; Macquarie Chemical Proteomics and Phosphoproteomics; University, North Ryde, Sydney, Australia Andreas Tebbe; Marc Kaminsk; Sebastian Wandinger; WP 148 Large Scale Quantitative Proteomics of Maize Christian Eckert; Stefan Müller; Christoph Schaab; Bundle Sheath and Mesophyll Chloroplasts Using the Klaus Godl; Martin Klammer; KINAXO First Maize Genome Assembly; Friso Giulia; Wojciech Biotechnologies, Munich, Germany Majeran; Mingshu Huang; Qi Sun; Klaas J. van Wijk; WP 137 Phosphoproteome of PTEN-Null and PTEN- Cornell University, Ithaca, NY Expressing U-87 MG Human Glioblastoma Cells WP 149 Large Scale Label-Free Quantitative Proteomics of Using a Phosphochip Coupled to a QTOF and ETD an Obligate Biosymbiotic System; Anton Poliakov; trap; Vaibhav Chumbalkar1; Vadiraja Bhat 2; Khatri Calum Russell; Angela Douglas; Klaas J. Van Wijk; Latha1; Oliver Bogler1; 1UT MD Anderson Cancer Cornell University, Ithaca, NY Center, Houston, TX; 2Agilent Technologies, WP 150 Protein Correlation Profiling in Combination with Wilmington, DE Blue Native PAGE Applied to Structural Analysis of WP 138 High Resolution Mass Spectrometry Based Profiling the Proteasome in Plasmodium falciparum; Nicole of Phosphoproteome in Breast Epithelial Cells with Sessler1, 3; Karsten Krug1; Alfred Nordheim2; Benjamin PIK3CA Knock-In Mutants; Xinyan Wu; Raghothama Mordmüller3; Boris Macek1; 1Proteome Center Chaerkady; Jun Zhong; Yi Yang; Kumaran Kandasamy; Tübingen, University of Tübingen, Tübingen, Germany; Min-Sik Kim; Ben Ho Park; Akhilesh Pandey; Min 2Department of Molecular Biology, Univ. of Tübingen, Ling; Johns Hopkins University, Baltimore, MD Tübingen, Germany; 3Institute of Tropical Medicine, WP 139 Identification and Functional Characterization of Univ.of Tübingen, Tübingen, Germany Pheromone Induced Fus3 Specific Phosphorylation WP 151 Survey of Estrogen-Induced Differential Protein Sites of the Yeast 26S Proteasome by Quantitative Expression in vivo from Mouse Brain Mitochondria Mass Spectrometry; Robyn Kaake; Lan Huang; Using LC-MS/MS and Label-Free Relative University of California, Irvine, CA Quantitation; Tatjana Talamantes; Vien Nguyen; QUANTITATIVE PROTEOMICS III, 140 - 163 Laszlo Prokai; University of North Texas Health Science WP 140 Quantitation of Insoluble/Low Level Escherichia coli Center, Fort Worth, TX WP 152 Colistin Resistant Acinetobacter baumannii Exhibits Proteins Under Acid Adaptation Conditions Using 1 15 Unique Protein Profile; Sara E. Whitson ; E. Ricky N Labeling, Subtractive Protein Solubilization and 1 2 3 ProteaseMAXTM Digestion; Grzegorz Sabat; Gregory Chan ; Giridharan Gokulrangan ; Sergei Ilchenko ; Mark D. Adams1; Masaru Miyagi 3; Mark Chance3; Barrett-Wilt; Heather L. Burch; Michael R. Sussman; 1 Amy C. Harms; University of Wisconsin, Madison, WI Case Western Reserve University School Of Medicine, Cleveland, OH; 2Case Center for Proteomics, Cleveland, WP 141 Construction of a Protein Assembly Timeline for 3 Spliceosomes by Relative MS-Based Quantification; OH; Case Western Reserve University, Cleveland, OH Carla Schmidt; Monika Raabe; Klaus Hartmuth; Mads WP 153 Quantitative Label Free Proteomics Approach for Gronborg; Reinhard Luhrmann; Henning Urlaub; MPI Understanding the Molecular Mechanisms of for Biophysical Chemistry, Goettingen, Germany Cellular Response against Stress; Nathan Smith; WP 142 How Deep Can We Go? Stable-Isotope Labeling for Christophe Espirito Santo; Sathish Kumar Natarajan; Quantitative Proteomics of Neuronal Cells; Martin Janani Prahlad; Jaekwon Lee; Gregor Grass; Mark Hornshaw1; Jenny Ho1; Ian Gilmore2; William James Wilson; Donald Becker; Renu Nandakumar; M.P. Griffiths2; Yuqin Wang2; 1Thermo Fisher Scientific, Nandakumar; University of Nebraska, Lincoln, NE Hemel Hempstead, UK; 2Swansea University, Swansea, WP 154 Proteomics, Ultrastructure and Physiology of UK Hippocampal Synapses in a Mouse Model of Fragile X Syndrome Reveals a Pre-Synaptic Phenotype; WP 143 Use of Multiplex SILAC Labeling to Study NT3 1 1 Signaling in Primary Neurons; Guoan Zhang1; Katrin Patricia Klemmer ; Roel van der Schors ; Rhiannon 1 1 2 1 Meredith3; Joke Wortel2; August B. Smit1; Ka Wan Li1; Deinhardt ; Moses Chao ; Thomas Neubert ; New York 1 University, New York, NY; 2Skirball Institute, NYUMC, Dept. Mol. & Cell. Neurobiology, CNCR, NCA, VU, , Netherlands; 2Dept. Func. Genomics, New York, NY 3 WP 144 Label-Free Quantitative Proteomic Studies on an CNCR, NCA, VU, Amsterdam, Netherlands; Dept. Int. Environmentally Important Methanotroph; Nisha A. Neurophys., CNCR, NCA, VU, Amsterdam, Netherlands Patel; Konstantinos Thalassinos; Yin Chen; Andrew WP 155 Elucidation of Methyl Jasmonate Signaling in Brassica napus Guard Cells by LC MALDI; Aaron Crombie; Susan E. Slade; J. Colin Murrell; James H. 1 1 1 Scrivens; University of Warwick, Coventry, UK Booy ; Brigitte Simons ; Sean L. Seymour ; Mengmeng Zhu2; Sixue Chen2; 1AB SCIEX, Concord, Canada; WP 145 Proteomics of Adipose-Derived Human Stem Cells 2 under Hyperosmotic Treatment; Elizabeth S. Oswald; University of Florida, Gainesville, Florida Lewis M. Brown; J. Chloë Bulinski; Clark T. Hung; WP 156 Quantification of Beta Tubulin Isotypes with iTRAQ Columbia University, New York, NY Labelling and MALDI Analysis of Specific Peptides; WP 146 Characterizing Human Respiratory Cilia Using Data- Nicole Bec; Christian Larroque; IRCM/CRLC Val Independent LC/MSE Provides Component Level d'Aurelle P. Lamarque, Montpellier, France Protein Stoichiometry Measurements; Kevin WP 157 Quantitative Proteomics of Lycopene Effects on 1 2 1 Prostate Primary Epithelial Cells by LC-MS/MS; Xi Blackburn ; Kristin Thompson ; Michael Goshe ; 1 1 1 2 Lawrence Ostrowski2; 1NC State University, Raleigh, Qiu ; Yang Yuan ; Chenqi Hu ; Avani Vaishnav ; Larisa 2 Nonn2; Richard B. Van Breemen1; 1University of Illinois, NC; University of North Carolina at Chapel Hill, 2 Chapel Hill, NC Chicago, IL; University of Illinois Medical Center, WP 147 Proteomic Analysis of Environmental Stress; Chicago, IL Chumithri Gayani Gammulla; Karlie Neilson; Mehdi WP 158 Quantitative Proteomics Analysis of cAMP/protein Mirzaei; Sridevi Muralidharan; Dylan Xavier; Dana kinase A (PKA)-mediated Protein Changes in S49 58th ASMS Conference on Mass Spectrometry Page S99 WEDNESDAY POSTERS Lymphoma Cells; Yurong Guo1; Lingzhi Zhang2; Paul Peptidome.; Hans-Dieter Zucht; Sasa Koncarevic; Insel2; Susan Taylor1, 2; 1HHMI, San Diego, CA; Karsten Kuhn; Christian Baumann; Stefan Selzer; Marco 2Department of Pharmacology, UCSD, La Jolla, CA Schärfke; Petra Budde; Proteome Sciences R&D GmbH WP 159 Antimicrobial Histones in Gills of Perch and & Co. KG, Frankfurt, Germany Walleye: Identification by Mass Spectrometry; WP 170 Solving the "Arginine-Conversion Problem" in Lorraine Anderson; Richard Leino; University of SILAC by Genetic Engineering; Juri Rappsilber; Minnesota, Minneapolis, MN Claudia Bicho; Flavia de Lima Alves; Zhuo Chen; Ken WP 160 Quantitative Proteomics Profiling Using iTRAQ and Sawin; Wellcome Trust Centre for Cell Biology, SCX RP-RP LC-MS; Edward Lau1; Maggie P. Y. Edinburgh, UK Lam1; S. O. Siu1; Simon Lee2; Ivan Chu1; 1University of WP 171 Development of Quantitative Methods to detect Hong Kong, Hong Kong, Hong Kong; 2University of Trace Amounts of Hazelnut and Soy Allergens in Macau, Macau, China Food; Rowan L. Dobson 1; Sévérine Fourdrilis2; WP 161 Tissue-Based Identification of Renal Cell Carcinoma Stéphanie Kirsch 2; Dominique Baiwir2; Guy Maghuin- Markers; Olena Masui1; Leroi Desouza1; K W Michael Rogister1; Marie Louise Scippo1; Edwin De Pauw1; Siu1; George Yousef2; Nicole White2; Olga Krakovska1; 1Liege University, Liège, Belgium; 2University of Liege, 1York University, Toronto, Canada; 2St. Michael's Liege, Belgium Hospital, Toronto, Canada WP 172 A Novel Software Algorithm for High-Throughput WP 162 Proteomic Analysis of Aldosterone Regulated Optimization of Quantitative MS/MS Parameters for Changes in Endosomal Compartments; Manimalha Peptide Quantitation and Alteration/Modification Balasubramani; Guy Uechi; Emanuel Schreiber; Huamin Screening; Xavier Misonne1; Anthony Romanelli1; Wang; Robert Edinger; John Johnson; University of Kevin Shirey2; 1AB SCIEX, Framingham, MA; 2Sound Pittsburgh, Pittsburgh, PA Analytics, Niantic, CT WP 163 Ultrathroughput Targeted Quantitative Proteomics WP 173 Using Mass Spectrometry to Speed Up the for Measuring Plasma Membrane CFTR Expression; Production of Influenza Vaccine and Ensure the Bekim Bajrami; Xudong Yao; University of Connecticut, Quality of the Final Product; Tracie L. Williams; Storrs, CT Carrie L. Pierce; James L. Pirkle; John R. Barr; Centers PEPTIDES: QUANTITATION METHODS, 164 - 192 for Disease Control and Prevention, Atlanta, GA WP 164 Comparison of SISCAPA, SCX, and SCX + Ion WP 174 Quantitation Analysis of Multiply Charged Small Funnel MS Coupled with SID-MRM-MS for the Peptides Using Liquid Chromatography/Targeted- Verification of Protein Biomarker Candidates; Enhanced Multiply Charged Scan in a Hybrid 1 Regine M. Schoenherr1; Hasmik Keshishian2; Tao Liu3; Quadrupole Linear Ion Trap.; Changtong Hao ; J.C. 2 2 1 Terri A. Addona2; Li-Chia Feng1; Lei Zhao1; Jeffrey R. Yves Leblanc ; J. Larry Campbell ; Udo Verkerk ; K W 1 1 2 Whiteaker1; Michael Burgess2; Eric Kuhn2; Mahmud Michael Siu ; York University, Toronto, Canada; AB Hossain3; Errol W. Robinson3; David G. Camp, II3; SCIEX, Concord, Canada Richard D. Smith3; Steven A. Carr2; Amanda G. WP 175 Search Algorithm for Determining Differences in the Paulovich1; 1Fred Hutchinson Cancer Research Center, MS/MS Spectra of Aspartate Isomers in Amyloid- 2 2 Seattle, WA; 2Broad Institute, Cambridge, MA; 3Pacific Beta Peptides; Maria I. Indeykina ; Igor Popov ; Anton 3 1 4 Northwest National Laboratory, Richland, WA Grigoriev ; Alexey Kononikhin ; Sergey Kozin ; Eugene 1 1 WP 165 Analytical Challenges in the Development of a Nikolaev ; The Institute for Energy Problems of 2 Quantitative SPE/LC/MS/MS Assay for Amyloid Chemical Phys, Moscow, Russian Federation; Institute 3 Beta Peptides in Cerebrospinal Fluid; Erin E. of Biochemical Physics RAS, Moscow, Russia; Moscow Chambers1; Mary E. Lame2; Diane M. Diehl1; Yanhua Institute of Physics and Technology, Moscow, Russia; 4 Zhang3; 1Waters Corporation, Milford, MA; 2Pfizer Institute of Biomedical Chemistry RAMS, Moscow, Neuroscience Research Unit, Groton, CT; 3Pfizer PDM, Russia Groton, CT WP 176 Evaluation of Quantification Consistency Using WP 166 Identification of Disease Biomarker Candidates Forward and Reverse Differential Isotope Labeling Using Subcellullar Fractionation Coupled to of Proteome Digests; Andy Lo; Joel H. Weiner; Liang Metabolic Labeling of Mouse Model of Alcoholic Li; University of Alberta, Edmonton, Canada Liver Disease; Prince Tieku1; Christine Wu 1; Hide WP 177 Development of an LC-MS/MS Method for 1 Tsukamoto2; 1University of Colorado School of Quantification of P-glycoprotein; Tasso Miliotis ; 1 2 Medicine, Aurora, CO; 2University of Southern Constanze Hilgendorf ; Peter Abrahamsson ; Tommy B 1 1 1 California, Los Angeles, CA Andersson ; Martin Ahnoff ; AstraZeneca R&D 2 WP 167 Application of Dried Blood Spots for the Quantitative Molndal, Molndal, Sweden; Agilent technologies, Assessment of Biologics; Jonathan Kehler; Chester L Gothenburg, Sweden Bowen; Christopher A. Evans; GlaxoSmithKline, King WP 178 An FTICR – Ion Trap Cluster for High Throughput Of Prussia, PA Label-Free Quantitative Proteomics; Magnus WP 168 Effect of Collision Energy Optimization on the Palmblad; Yuri E.M. Van Der Burgt; Hans Dalebout; Measurement of Peptides by Selected Reaction Ekaterina Mostovenko; André M. Deelder; Leiden Monitoring (SRM) Mass Spectrometry; Daniela University Medical Center, Leiden, Netherlands Tomazela1; Brendan Maclean1; Susan E. Abbatiello2; WP 179 Investigation of Peptide loss in MRM-Experiments Steven A. Carr2; Michael J. Maccoss1; 1University of during Absolute Protein Quantification; Olaf Washington, Seattle, WA; 2Broad Institute, Cambridge, Boernsen; Rafael Sande; Denis Herzog; Stephan Bek; MA Novartis Pharma AG, Basel, Switzerland WP 169 Tandem Mass Tag (TMT) Labelling Developed for WP 180 Investigating Ion Mobility Coupled with Time-of- Peptidomics of Cerebrospinal Fluid: Investigating Flight Mass Spectrometry and Liquid Preanalytical Sample Handling Influencing the CSF- Chromatography for the Parallel Sequencing of Page S100 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS Labelled Isobaric Peptides; Jim Langridge1; Chris the Extracellular Matrix; Kirk Hansen; Lauren Hughes 1; Marc V. Gorenstein1; Scott Geromanos1; Kiemele; Jenean Obrien; Pepper Schedin; Univ. of CO. Johannes Pc Vissers1; Thérèse Mckenna1; Dan Golick1; Denver, AMC, Aurora, CO 2 1 Bernhard Kuster ; Waters Corporation, Manchester, PEPTIDES: FRAGMENTATION AND SEQUENCING, 2 UK; Technical Universitiy Munich, Freising, Germany 193 - 212 WP 181 Subtle Modification of Isotope Ratio Proteomics 13 12 WP 193 Does Scrambling of b-Ions Lead to Incorrect (SMIRP) through Manipulation of C/ C ratio in E. Assignments of Protein Sequences?; Irine Saminathan; coli and the Development of IsoArt; Puneet Souda1; 1 1 2 Alan Hopkinson; K W Michael Siu; CRMS, Chemistry, Arthur Laganowsky ; Sara Bassilian ; Jonathan Katz ; York University, Toronto, Canada Julian Whitelegge1; 1University of California Los 2 WP 194 Multiple Structures of b-Ions Probed by Electron Angeles, Los Angeles, CA; University of Southern Capture Dissociation; Cheng Lin 1; Xiaojuan Li1; Peter California, Los Angeles, CA O'Connor2; 1Boston University School of Medicine, WP 182 The use of Dual cHiPLC Columns to Increase Boston, MA; 2Warwick University, Coventry, UK Throughput in Quantitative Peptide MRM Analyses; WP 195 Sequencing of Phosphopeptide Anions: Negative J.Bryce Young; Nicole Hebert; Erika Lin; Remco Van Electron Transfer Dissociation and IR-MPD; Israel Soest; Eksigent Technologies, Dublin, CA Ugalde; Nicolas Polfer; University of Florida, WP 183 Delving Deeper - A Comparative Proteomic Analysis 1 Gainesville, FL of Depleted Suction Blister Fluid; André Müller ; WP 196 Characterizing Negative Electron Transfer Keiryn Bennett1; Oliver Brandt2; Florian P. Breitwieser1; 1 2 1 Dissociation (NETD) Induced Neutral Losses; Neil Jacques Colinge ; Georg Stingl ; Giulio Superti-Furga ; Rumachik; Graeme McAlister; Joshua J. Coon; Adelheid Elbe-Bürger2; 1CeMM - Center for Molecular 2 University of Wisconsin, Madison, WI Medicine, Vienna, Austria; Dept. Dermatology, Med. WP 197 ExD Fragmentation of Modified Peptides Containing Uni. Vienna, Vienna, Austria Beta Amino Acids Including Isoaspartate and WP 184 A New Automated Approach for the Determination Various Beta and Cyclic Beta Amino Acids; Nadezda of Peptides Using On-Line SPE-LC-MS/MS; John P. Sargaeva1; Cheng Lin 1; Konstantin Aizikov1; Crutchfield2; Martin Sibum1; Emile Koster1; 1Spark 1 1 1 2 Chunxiang Yao ; Xiang Yu ; Xiaojuan Li ; Peter B. Holland, Emmen, Netherlands; Spark Holland Inc., O'connor2; 1Boston University School of Medicine, Brick, NJ Boston, MA; 2University of Warwick, Coventry, UK WP 185 Comparison of Triple Quadrupole, Ion Trap and WP 198 Comparison of Hot ECD with ECD and AI-ECD in Orbitrap Parameters Required for Optimal Absolute Radio Frequency Ion Trap Using Mascot MS/MS Protein Quantitation; David M. Schieltz1; Sara C. 1 2 1 1 Search; Hiroyuki Satake; Akihito Kaneko; Naomi Mcgrath ; Lisa G. Mcwilliams ; John R. Barr ; Centers Manri; Atsumu Hirabayashi; Yuichiro Hashimoto; for Disease Control and Prevention, Atlanta, GA; 2 Takeshi Sakamoto; Hitachi, Ltd., Central Research Battelle Memorial Institute, Atlanta, GA Laboratory, Kokubunji, Tokyo, Japan WP 186 Ultra High-Resolution, UHR-TOF Coupled with WP 199 Gas-Phase Fragmentation of [M+nH+OH]n•+ Ions UHPLC for Quantitative Determination of Peptides Formed from Peptides Containing Intra-Molecular and Small Molecule Drugs in Biological Matrix; Disulfide Bonds; Xiaoxiao Ma; Yu Xia; Purdue Hongwei Zhang; Baomin Xin; Asoka Ranasinghe; Qian University, West Lafayette, IN Ruan; Li Ma; Mingshe Zhu; Timothy Olah; Bristol- WP 200 New Algorithm for the Identification of Intact Myers Squibb, Princeton, NJ Disulfide Linkages Based on Fragmentation WP 187 The Dynamic Range of MS/MS-based Label-Free Characteristics in Tandem Mass Spectra; Seonhwa Quantification in Ion Trap Instruments; Alexander Choi1; Jaeho Jeong2; Seungjin Na1; Hyo Sun Lee2; Hwa- Scherl; HuiSong Pak; Patrizia Arboit; Carla Pasquarello; Young Kim3; Kong-Joo Lee2; Eunok Paek1; 1University University of Geneva, Geneva, Switzerland of Seoul, Seoul, South Korea; 2Ewha Womans WP 188 Bioanalytical Challenges and Strategies used to University, Seoul, South Korea; 3Yeungnam University Develop a Highly Sensitive LC/MS/MS-based Method College of Medicine, Daegu, South Korea for Peptides in Support of a Discovery Program; WP 201 Selective C-I Bond Cleavage in ECD: Prospects for Hongwei Zhang; Baomin Xin; Christian Caporuscio; Designing Novel Chemical Cross-Linker and Timothy Olah; Bristol-Myers Squibb, Pennington, NJ Mechanistic Implications; Bo Wang; Cynthia Cipolla; WP 189 Robust and Sensitive Relative Quantitation of TMT Kristina Hakansson; University of Michigan, Ann Arbor, Labeled E. coli Digest by Dual Cell Linear Ion Trap- MI Orbitrap Hybrid Mass Spectrometer; Terry Zhang; WP 202 Tandem MS, Isotope Labeling, and DFT Study of Rosa Viner; Vlad Zabrouskov; David Horn; Model Phosphotripeptides; Sarah Young; Ryan Dain; ThermoFisher, San Jose, CA Sam Molesworth; Michael J. Van Stipdonk; Wichita WP 190 A Novel Approach for Quantitative Peptides Analysis State University, Wichita, KS by Selected Electron Transfer Reaction Monitoring; WP 203 Effect of Basic Residues on Selective CĮ–C Bond Chien-Chen Lai; Bi-Ying Wei; National Chung Hsing Cleavages of Peptide Radical Cations; Tao Song1; University, Taichung, Taiwan Minjie Xu1; Quan Quan1; C. K. Siu2; Decai Fang3; Ivan WP 191 An On-Line Solid Phase Extraction LC-MS/MS K. Chu1; 1The University of Hong Kong, HK, China; Method for the Simultaneous Determination of 2City University of Hong Kong, HK, China; 3Beijing Hepcidin Isoforms in Human Serum; Takahide Normal University, Beijing, China Uchimura; Tomonori Kamei; Kazuo Tokuda; Kazuhiro WP 204 The Theoretical Calculations of Relative Ion Nishimiya; Takehiko Kawanishi; Chugai Intensities in MSMS Spectra of Doubly Charged Pharmaceutical Co.,Ltd, Kamakura, Kanagawa, Japan Penta-Peptides; Tibor Pechan; Steven Gwaltney; WP 192 Comparison of Two Label-Free Quantification Mississippi State University, Mississippi State, MS Methods; Monitoring Involution Induced Changes in 58th ASMS Conference on Mass Spectrometry Page S101 WEDNESDAY POSTERS + + WP 205 Structural Characterization of b2 , [M+H-17] , and WP 217 Phospho-Tau SRM – A Multi-Site Phosphorylation [M+H-18] + From Glutamine Containing Tripeptides Assay for Tau Protein in Pre-Clinical Models of Using IRMPD Spectroscopy, DFT, and Tandem Mass Alzheimer’s Disease and Other Neurological Spectrometry; Sam Molesworth1; Sarah Young1; Disorders; Malcolm Ward; Helen Byers; James Jeffrey Steill2; Jos Oomens2; Michael J. Van Stipdonk 1; Campbell; Emma Schofield; Proteome Sciences Plc, 1Wichita State University, Wichita, KS; 2FOM London, UK Rijnhuizen, Nieuwegein, Netherlands WP 218 Developing and Validating An Immunoaffinity LC- WP 206 Density Functional Theory -- Based Predictions of MS/MS Assay for the Quantitation of Matrix Ion Intensity Relationships in Mass Spectra of Metalloproteinase 9 in Mouse Serum; Mireia Oligoalanines; Oleg Obolensky 1; Wells Wu 2; Rong- Fernandez Ocana1; Hendrik Neubert2; 1Pfizer, Sandwich, Fong Shen3; Yi-Kuo Yu1; 1National Center for UK; 2Pfizer Inc., Sandwich, UK Biotechnology Information, NLM, Bethesda, MD; 2NIH WP 219 Direct Quantitation of Hydroxyethylvaline in (NIA), Baltimore, MD; 3NIA/NIH, Baltimore, MD Hemoglobin by Liquid Chromatography/ Positive WP 207 Computational and Experimental Study of Bond Electrospray Tandem Mass Spectrometry; Fagen Cleavage Mechanisms in phospho-serine Peptide Zhang; Kathy A. Brzak; Michael J. Bartels; The Dow ECD and ETD Mass Spectrometry; Christopher Moss; Chemical Company, Midland, MI Thomas W. Chung; Frantisek Turecek; University of WP 220 LC/MRM Quantification of Protein-Bound Washington, Seattle, WA Chlorotyrosine and Bromotyrosine in Serum of WP 208 Carbonyl vs. Side-Chain Directed Nucleophilic Patients Infected by Inflammatory Bowel Disease; Yu Attacks in Peptide Collision-induced Dissociation; Zeng1; John S. Wishnok1; Joshua Korzenik2; Steve Shen Zou1; Warren K Mino1; Xian Chen1; Jeffrey Steill2; Tannenbaum1, 3; 1MIT, Cambridge, MA; 2Massachusetts Jos Oomens2, 3; Nicolas Polfer1; 1University of Florida, General Hospital, Boston, MA; 3Department of Gainesville, FL; 2FOM Rijnhuizen, Nieuwegein, Chemsitry, MIT, Cambridge, MA Netherlands; 3University of Amsterdam, Amsterdam, WP 221 Discovering Oral Squamous Cell Carcinoma Netherlands Biomarkers via Novel, Non-Invasive in-situ of Oral WP 209 Residue Dependence of Observable Modified b1 Ions Lesion Exudates and Proteomic Analysis; Joel for Proteome Digests Labeled with Substituted Kooren; Chuanning Tang; Nelson Rhodus; Matthew Phenylisothiocyanates; Pamela Ann Diego; Xudong Stone; Tim Griffin; University of Minnesota, Yao; University of Connecticut, Storrs Mansfield, CT Minneapolis, MN WP 210 Specific N-Terminal Labeling and Its Application on WP 222 LC/MS/MS Quantitation of CA125 in Human Urine Protein Mixture Analysis; Suping Zheng; Min Bian; for Ovarian Cancer Diagnosis and Prognosis; Bob Steven Becht; Xiaoya Ding; PPD, Inc., Middleton, WI Xiong1; Lily Li2; Patrick Bennett1; Bin Ye3; Daniel WP 211 Effect of Basic Amino-Acid Residue on Radical Cramer3; 1Tandem Labs, Woburn, MA; 2TandemLabs, Migration and Dissociation of Į-Carbon-Centered Woburn, MA; 3Brigham and Women’s Hospital, Boston, Glycylarginyltryptophan Radical Cation; Dominic MA Chun Ming Ng1; Tao Song1; Chi Kit Siu2; Ivan Keung WP 223 Assay for the Simultaneous Measurement of Multiple Chu1; 1The University of Hong Kong, Hong Kong, Amyloid Beta Isoforms; Kwasi Mawuenyega; Randall China; 2City University of Hong Kong, Hong Kong, Bateman; Washington University School of Medicine, China Saint Louis, MO WP 212 A Comparison of 193 nm Photofragment ion Spectra WP 224 Development and Implementation of a TMT-SRM Probing Fragment Ions Formed on Distinct Assay for the Qualification of Candidate Biomarkers Timescales; Kevin Kmiec; David H. Russell; Texas of Alzheimer’s Disease; Darragh O'Brien1, 2; Andreas A&M University, College Station, TX Güntert2; Karsten Kuhn3; Malcolm Ward1; Simon 2 1 1 1 BIOMARKDER ASSAYS, 213 - 236 Lovestone ; James Campbell ; Helen Byers ; Proteome Sciences Plc, London, UK; 2Institute of Psychiatry, WP 213 An MRM assay for HAPLN1 - A Protein with 3 Potential as a Prognostic Marker for Malignant King's College London, London, UK; Proteome Pleural Mesothelioma (MPM); Sofia Waldemarson1; Sciences R&D GmbH&CoKG, Frankfurt am Main, Chandra M.V. Goparaju1; Asa Wahlander1; Steven Germany Blais1; David Fenyo2; Harvey I. Pass1; Thomas WP 225 Mass Spectrometric Study of the Highly Selective Neubert1; 1New York University Medical Center, New Derivatization of Biological Thiols by Selenium 2 Reagents; Kehua Xu1, 2; Yun Zhang 1; Bo Tang2; Julia York, NY; The Rockefeller University, New York, NY 3 3 1 1 WP 214 Determination of Lipoxygenase Products of Laskin ; Patrick Roach ; Hao Chen ; Ohio University, Athens, OH; 2Shandong Normal University, Jinan, Arachidonic Acid in Tissue and Plasma by LC- 3 MS/MS; Kris King; Ling Morgan; Guangyu Zhao; Chris China; Pacific Northwest National Laboratory, Tran; Tandem Labs, Woburn, MA Richland, WA WP 215 Delipidation of ApoB48 and ApoB100 for Fast WP 226 Synthesis of Stercobilin and its Deuterated Isotopomer: ESI and MS/MS of a Potential Autism Quantitation by LC-ESI/MS/MS; Theresa Mclaughlin; 2 1 Michael Lassman; Elizabeth Polizzi Somers; Zhu Chen; Biomarker; Dr. Troy Wood ; Dr. Amber Charlebois ; Gregory Pirrone1; 1Faileigh Dickinson University, Thomas Roddy; Merck Research Labs, Rahway, NJ 2 WP 216 The Albuminome: Converting Proteomic-Based Madison, NJ; SUNY University at Buffalo, Buffalo, NY Discovery into Biomarker Validation; Christine A. WP 227 Open-Platform and Targeted MRM Approaches for 1 2 2 Prediction of INF/Ribavirin Treatment Response in Jelinek ; Rebekah L. Gundry ; Jennifer E. Van Eyk ; 1 2 Robert J. Cotter1; 1Johns Hopkins School of Medicine, Hepatitis C Patients; Laura Dubois ; Keyur Patel ; 2 Joseph Lucas1; J. Will Thompson 1; Jeanette McCarthy1; Baltimore, MD; Johns Hopkins University, Baltimore, 2 1 1 MD John McHutchison ; Arthur Moseley ; Duke Institute Page S102 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS 2 for Genome Sciences and Policy, Durham, NC; Duke PROTEOMICS PTM DETERMINATION: Clinical Research Institute, Durham, NC PHOSPHORYLATION & GLYCOSYLATION, 237 - 254 WP 228 PhosphoScan Direct: A Novel Method for WP 237 Combining Protein- and Peptide-Level Enrichment Quantitative Monitoring of Signaling Pathways; for the Determination of Minor Human Milk Protein Charles L. Farnsworth; Matthew P. Stokes; Xiaoying Jia; Phosphorylation; John Froehlich1; Ning Tang2; Keith Jian-Min Ren; Joan MacNeill; Christopher Bunker; John Waddell2; Carlito Lebrilla1; 1University of California, Rush; Jeffrey C. Silva; Cell Signaling Technology, Davis, CA; 2Agilent Technologies, Santa Clara, CA Danvers, MA WP 238 A New Method to Identify Phosphoproteins WP 229 Practice of Protein Biomarker Assay Development Quantitatively and in Real Time Using 2D-DIGE; Sun and Validation From Drug Discovery to Yong Jeong 1, 2; Cristina Osorio2; Robert DeKroon1, 2; Development; Lily Li; Bob Xiong; David Ho; Patrick Eric Hamlett1; Jennifer Robinette2; Mihaela Mocanu2; Bennett; TandemLabs, Woburn, MA Oscar Alzate1, 2; 1Department of Cell and WP 230 Development of an Optimized ProteaseMAX Assisted Developemental Biology, Chapel Hill, NC; 2UNC- Trypsin Digestion of Human CSF for SRM Systems Proteomics Center, Chapel Hill, NC Quantification of GFAP and Identification of 1 1 WP 239 Evaluation of a Protein Phosphorylation Biomarkers.; Robert Cunningham ; Dustin Frost ; Determination Method Using a MALDI-TOF/TOF Albee Messing2; Lingjun Li1; 1Univ. of Wisconsin- 2 Mass Spectrometer; Jhoana Mendoza; Rong Wang; Maidson, Madison, WI; Waisman Center, Univ. of Mount Sinai School of Medicine, New York, NY Wisconsin-Madison, Madison, WI WP 240 Unspecific Products of Specific Cleavage: WP 231 Evaluation of a Large Set of Quantitative Peptide Implications for Phosphorylation Analysis.; Anna Affinity-Based Mass Spectrometric Assays; Jeffrey Shevchenko; Henrik Thomas; Andrea Knaust; Andrej Whiteaker1; Lei Zhao1; Eric Kuhn2; Susan E. 2 3 4 Shevchenko; MPI of Molecular Cell Biology and Abbatiello ; Matthew Pope ; Angela Jackson ; Michael Genetics, Dresden, Germany Burgess2; Leigh Anderson5; Terry Pearson3; Steven A. 2 1 1 WP 241 Top-Down High Resolution Electron Capture Carr ; Amanda Paulovich ; Fred Hutchinson Cancer Dissociation Mass Spectrometry for Characterization Research Center, Seattle, WA; 2Broad Institute, 3 of Post-Translational Modifications in Non-Human Cambridge, MA; University of Victoria, Victoria, Primate Cardiac Troponin; Fangmin Xu; Lisa Xu; British Columbia, Canada; 4UVic Genome BC 5 Xintong Dong; Timothy Hacker; Ying Ge; University of Proteomic Centre, Victoria, BC; Plasma Proteome Wisconsin-Madison, Madison, WI Institute, Washington, DC WP 242 Functional Study of BR-Signalling Kinases (BSKs) in WP 232 Peptide Choice for MRM3 Quantification in Clinical Arabidopsis; Shouling Xu1, 2; Peng Xu1; Robert Evaluation of Biomarkers; Tanguy Fortin1; Arnaud 2 2 2 2 1 Chalkley ; Shenheng Guan ; A.L. Burlingame ; Zhiyong Salvador ; Jean-Philippe Charrier ; Geneviève Choquet- Wang1; 1Carnegie Institution for Science, Stanford, CA; Kastylevsky1; Jérôme Lemoine2; 1BIOMERIEUX, Marcy 2 2 University of California, San Francisco, CA L'etoile, France; University of Lyon (UCBL-1), Lyon, WP 243 Improved Phosphoproteome Analysis by Multi- France Enzymatic Digestions Targeting the Trypsin- WP 233 IEF-Chip/HPLC-Chip-SRM-MS: Quantification of Resistant Proteome (TReP); Bao Tran1; Celine Proteins Using Chip-Based Enrichment/Separation Hernandez1, 2; Alexandra Potts1; Patrice Waridel1; with Selected Reaction Monitoring MS and Stable Frederique Lisacek2; Manfredo Quadroni1; 1University of Isotope Dilution; Agnes Rafalko; Shujia Dai; William Lausanne, Lausanne, Switzerland; 2Swiss Institute of Hancock; Barry L. Karger; Marina Hincapie; Bioinformatics, Geneva, Switzerland Northeastern University, Barnett Institute, Boston, MA WP 244 FeOx, ZrOx, HfOx and TiOx Nano-Enhanced WP 234 Screening for Drug-Drug Interactions Using a MALDI Plates for Selective Capture of Targeted Proteomics Strategy; Daniel B. Kassel1; 1 1 2 Phosphorylated and Sialylated Peptides.; Paolo Kheng B. Lim ; Melinda Manuel ; Teruaki Okuda ; Soffientini1; Andrea Di Fonzo1; Emanuele Barborini2; Naomi Kamiguchi2; Christie L Hunter3; Brian 2 2 3 3 3 1 Roberta Carbone ; Simone Vinati ; Gabriela Grigorean ; Williamson ; Lydia Nuwaysir ; Takeda San Diego, Inc, 1COGENTECH, Milan, Italy; 2Tethis s.r.l., Milan, Italy; San Diego, CA; 2Takeda Pharmaceutical Company, 3 3 IFOM-IEO, Milan, Italy Limited, Osaka, Japan; Applied Biosystems, Foster WP 245 Large-Scale Phosphoproteomics with High Mass City, CA Accuracy at the MS and MS/MS Level Using HCD on WP 235 Evaluating Blood Collection Tubes for Plasma an LTQ Orbitrap Velos; Nagarjuna Nagaraj1; Rochelle Protein Biomarker Discovery Using Selected D'souza1; Juergen Cox2; Jesper V Olsen3; Matthias Reaction Monitoring Mass Spectrometry; Sarah Mann4; 1MaxPlanck Institute for Biochemistry, Munich, Randall1, 2; Matthew McKay1, 2; Mark Molloy1, 2; 1APAF, 2 2 Germany; Max-Planck-Institute of Biochemistry, Sydney, Australia; Macquarie University, Sydney, Martinsried, Germany; 3Copenhagen University, Australia Copenhagen, Denmark; 4Max Planck Institute for WP 236 Development of a Quantitative SRM Assay for Biochemistry, D Martinsried, Germany Histone H2AX Ser139 Phosphorylation, a Key WP 246 Does Ion-trap CID Intramolecular Phosphate Regulator of the DNA Damage Response (DDR); Transfer Compromise Site Localization in Large- Mary F Lopez1; Victoria Lunyak2; Amol Prakash3; 4 5 1 Scale Phosphoproteomics Studies? Is ETD a Viable Michael Athanas ; Taha Rezai ; Bryan Krastins ; David Alternative?; Mike Aguiar1; Wilhelm Haas1; Sean Sarracino 5; 1ThermoFisher, Cambridge, MA; 2Buck 1 2 1 1 3 Beausoleil ; John Rush ; Steven Gygi ; Harvard Institute for Age Research, Novato, CA; ThermoFisher Medical School, Boston, MA; 2Cell Signaling Scientific, Cambridge, MA; 4VAST Scientific, Wayland, 5 Technology, Danvers, MA MA; Thermo Fisher Scientific, San Jose, CA WP 247 Highly Efficient Online Enrichment Method for the Selective Analysis of Phospho-Epitopes in Complex 58th ASMS Conference on Mass Spectrometry Page S103 WEDNESDAY POSTERS HLA Elution Samples; Geert Mommen; Ad de Jong; MALDI-TOF-MS; Ömür Çelikbçak; Bekir Salih; Netherlands Vaccin Institute, Bilthoven, The Netherlands Hacettepe University, Ankara, Turkey WP 248 Human Beta Defensin 3 Alters Activated T Cell WP 258 Structural Characterization of Aȕ Protein Responses Via Tyrosine Phosphorylation of STAT1; Crosslinking Using Ion Mapping and Tandem Mass Xiaolin Li; Jeffrey Meisch; Daniela M. Schlatzer; Giri Spectrometry; Jennifer G. Bryant1; William M. Tay2; Gokulragan; Janna Kiselar; Alan D. Levine; Mark R. A. Jeremy Nix2; Patricia K. Martin2; Terrone L. Chance; Case Western Reserve University, Cleveland, Rosenberry2; 1University of North Florida, Jacksonville, OH FL; 2Mayo Clinic, Jacksonville, Florida WP 249 Exploring the Human Leukocyte Phosphoproteome WP 259 Ecdysis Triggering Hormone Profiles in Endocrine Using a Microfluidic RP-TiO2-RP HPLC Inka Cells Using Single Cell MALDI-MS; Li Dai1, 2; Phosphochip Coupled to a Q-ToF Mass Michael Adams2; 1University of Utah, Salt Lake City, Spectrometer; Reinout Raijmakers1; Karsten Kraiczek3; UT; 2Depts. of Entomology, Cell Biology & Ad De Jong2; Shabaz Mohammed1; Albert J.R. Heck 1; Neuroscience, Riverside, CA 1Utrecht University, Utrecht, netherlands; 2Netherlands WP 260 Electron Ionization Mass Spectra Obtained for Vaccine Institute, Bilthoven, The Netherlands; 3Agilent Amino Acids and Peptides by Using Laser-Induced Technologies, Waldbronn, Germany Acoustic Desorption (LIAD); Benjamin C. Owen; WP 250 Large-Scale Enrichment and Analysis of Lindsey M. Kirkpatrick; Hilkka I. Kenttämaa; Purdue GlcNAcylated peptiDes from Arabidopsis Thaliana; University, West Lafayette, IN Sushmit Maitra1; Yung-Chun Kim2; Neil Olszewski2; WP 261 A Systematic Study for Determining Optimal Protein Jeffrey Shabanowitz1; Donald F. Hunt1; 1Dept. of Digest Conditions in Disulfide-Containing Proteins; Chemistry, University of Virginia, Charlottesville, VA; Kathryn Rebecchi; Eden Go; Heather Desaire; 2Dept of Plant Biology, University of Minnesota, St. University of Kansas, Lawrence, KS Paul, MN WP 262 Measurement of Major Urinary Protein Synthesis WP 251 Characterizing Post-Translational Modifications by Rate in Mice by Heavy Water Labeling and Peptide Mass Spectrometry for Protein Specific Activity Mass Spectrometry.; Mahalakshmi Rudrabhatla1; Study; David Wong; Marina Chow; Bill Cuevas; Jackie Mahalakshmi Shankaran1; Tom Hirst1; Scott Turner1; Huitink; Mariliz Johnson; Pooja Patel; Matt Reboli; Robert Beynon2; Richard Neese3; Marc Hellerstein3; Genencor Division of Danisco US Inc., Palo Alto, CA 1Kinemed, Inc., Emeryville, CA; 2University of WP 252 Differential Mass Spectrometric Analysis of Paxillin Liverpool, Liverpool, UK; 3Nutritional Science and O-glcNAcylation Using Chemical Labeling and Toxicology, UC Berkeley, Berkeley, CA IMAC Enrichment; Erin D Jeffery; Pablo Grigera; J. WP 263 Selective ESI-MS Detection of Basic Peptides Using Thomas Parsons; Donald F. Hunt; University of Virginia, Crown Ethers and Proton Transfer Reactions; Charlottesville, VA Michelle Sweeney; Megan Macnaughtan; Louisiana WP 253 Progress in the Characterization of Glycosylation State University, Baton Rouge, LA Microheterogeneity in Xenopus laevis Vitelline PROTEOMICS: SYSTEMS BIOLOGY( NON GEL BASED), 1 Envelope Glycoprotein ZPA; Liang Zhao ; Fan 264 – 293 Xiang2; Jerry L. Hedrick3; Andreas H. Franz1; 1 WP 264 A Systems Biology Approach to the Molecular Effects Department of Chemistry, University of the Pacific, Induced by Secondhand Smoke Exposure in Rat Pup Stockton, CA; 2Shimadzu Biotech, Pleasanton, CA; 1 1 1 3 Brain; Brian Fuller ; Andrew K. Ottens ; Virginia Department of Animal Science, UC Davis, Davis, CA Commonwealth University, Richmond, VA WP 254 Glycosylation and Sialylation of Macrophage- WP 265 Toxicological Pathway Protein Analysis Using Highly Derived Human Apolipoprotein E Analysed by 1- Multiplexed Multiple Reaction Monitoring (MRM) and 2-D SDS-PAGE and Mass Spectrometry.; Youra 2 1 2 2 Measurements; Hua Lin; Thomas A. Shaler; Sophia Lee ; Mark J. Raftery ; Wendy Jessup ; Maaike Kockx ; Chen; Chris Becker; Caprion Proteomics U.S. LLC, Renate Griffith3; Len Kritharides4; 1Bioanalytical Mass 2 Menlo Park, CA Spectrometry, Sydney, Australia; Centre for Vascular WP 266 Large Scale Quantitative Analysis of Adipose and Research, SOMS, UNSW, Sydney, Australia; Liver Tissue of Obese Mice Using an LTQ Orbitrap 3Pharmacology, SOMS, UNSW, Sydney, Australia; 1 2 4 XL; Dorothea Rutishauser ; Hadi al Hasani ; Ralf Department of Cardiology, Concord Hospital, Herwig3; Michal Okoniewski1; Jonas Grossmann1; Ralph University of Syndey, Sydney, Australia Schlapbach1; 1Functional Genomics Center, ETH PEPTIDES: GENERAL, 255 - 263 Zurich, Zurich, Switzerland; 2German Institute for WP 255 Structure, Reaction Intermediates and Human Nutrition, DIfE, Potsdam-Rehbruecke, Nuthetal, Topographical Characterization of ß-amyloid Germany; 3Max Planck Institute for Molecular Genetics, Oligomerisation Revealed by Ion Mobility Mass Berlin, Germany Spectrometry and Electron Paramagnetic Resonance WP 267 Quantitative Proteome Analysis of Pluripotent Cells Spectroscopy; Marius-Ionut Iurascu1; Claudia Cozma 1; by iTRAQ Mass Tagging Identifies Post- Michael Desor2; Malte Drescher1; Michael Przybylski1; Transcriptional Regulation of Proteins Required for 1University of Konstanz, Konstanz, Germany; 2Waters, ES Cell Self-Renewal; Robert N. O’Brien; Zhouxin Eschborn, Germany Shen; Kiyoshi Tachikawa; Angel Lee; Steven P. Briggs; WP 256 Synthesis and Structural Characterization of a University of California San Diego, La Jolla, CA Proteolytic Parkinson-Related Fragment of Į - WP 268 Profiling Human Transcriptional Regulators with Synuclein, Į -Syn(71-140); Kathrin Lindner; Camelia Shotgun Nucleoproteomics; Andrew Stergachis; Eric Vlad; Michael Przybylski; Universitat Konstanz, Rynes; Greg Finney; Michael MacCoss; John Konstanz, Germany Stamatoyannopoulos; University of Washington, Seattle, WP 257 Investigation of ȕ-Amyloid(1-40) Peptide Plaque WA Formation and Inhibition with Various Inhibitors by Page S104 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS WP 269 Quantitative Proteomics Analysis of Human WP 279 Virus and Host Proteomics of the Lactic Acid Adenovirus Infections by iTRAQ-8plex; Hung Viet Bacteria Streptococcus thermophilus and Its Phage Trinh1; Peter Gehrig2; Hansruedi Baetschmann2; Jonas D2972; Jacque Young1, 2; Manesh Shah1; Brian Dill1; Grossmann2; Ralph Schlapbach2; Urs Greber1; Silvio Philippe Horvath4; Christine Sun3; Christophe Fremaux5; Hemmi1; 1IMLS, University of Zurich, Zurich, Jillian Banfield3; Rodolphe Barrangou5; Nathan C. Switzerland; 2Functional Genomics Center, Zurich, Verberkmoes1; 1Oak Ridge National Labs, Oak Ridge, Switzerland TN; 2Univ. of Tennessee, Knoxville, TN; 3University of WP 270 Proteomic Analysis of 3t3-LI Adipocyte California, Berkeley, CA; 4Danisco France SAS, Dangé- Mitochondria during Differentiation, Maturation, Saint-Romain, France; 5Danisco USA, Inc., Madison, WI and Hypertrophic Enlargement; William K. Russell; WP 280 Expanding the Protein Library and Knowledge of Stephanie Cologna; Billy Newton; Arul Jayaraman; Relative Protein Abundances for M. Tuberculosis; David H. Russell; Texas A&M University, College Thomas A. Shaler1; Hua Lin1; Sophia Chen1; Julian Station, TX Richards2; James Galagan3; Chris Becker1; 1Caprion WP 271 A Systems Biology Approach; Proteomic Study on Proteomics U.S. LLC, Menlo Park, CA; 2Broad Institute, Rapamycin Treated Human Cancer Cell Line; Sudha Cambridge, MA; 3Boston University, Boston, MA Rajagopalan; Yugandhar Reddy; Nilanjan Guha; Siji WP 281 Elucidating the Parasitic/Symbiotic Association Joseph; Syed Salman Lateef; Agilent Technologies India between the Archaea ignicoccus hospitalis and Pvt.Ltd, Bangalore, India Nanoarchaeum equitans via Differential Proteomics.; WP 272 Global Analysis of Protein Expression Dynamics in Richard J. Giannone; Mircea Podar; Robert Hettich; Oak Human Tumor Cells upon Treatment with the Raf Ridge National Laboratory, Oak Ridge, TN Inhibitor Sorafenib; Sven Nahnsen1; Oliver WP 282 Protein Abundance Dynamics in the Unicellular Kohlbacher2; Alfred Nordheim3; Boris Macek1; Cyanobacterium Cyanothece ATCC 51142 During 1Proteome Center, University of Tübingen, Tübingen, Light-Dark Cycle; Uma K. Aryal1; Jon M. Jacobs1; Germany; 2Center for Bioinformatics, University of Jana Stöckel2; Carrie D. Nicora1; Himadri B. Pakrasi2; Tübingen, Tübingen, Germany; 3Department of David W. Koppenaal1; Louis A. Sherman3; Richard D. Molecular Biology, Univ. of Tübingen, Tübingen, Smith1; 1Biological Science Division, Pacific Northwest Germany Nat, Richland, WA; 2Department of Biology, Washington WP 273 Proteomic Profiling of Cellular Reprogramming: Are University, St Louis, MO; 3Dept. of Biological Sciences, Human Induced Pluripotent Stem Cells (hiPSCs) Purdue University, West Lafayette, IN Indistinguishable from Human Embryonic Stem WP 283 Identification of Host Species from the Blood Meal of Cells (hESCs)?; Javier Munoz1; Teck Yew Low1; Yee Tick Nymphs Using LC/MSMS; Ünige A. Laskay1; Jiun Kok2; Andre Choo2; Albert J.R. Heck 1; 1Utrecht Erin M. Johnson 1; Alan G. Barbour2; Vicki H. University, Utrecht, Netherlands; 2Bioprocessing Wysocki1; 1University of Arizona, Tucson, AZ; Technology Institute, Singapore, Singapore 2University of California Irvine, Irvine, CA WP 274 A Systems Biology View of the HeLa Proteome: WP 284 A Systems Biology Approach to Understanding in- Mapping 4000+ Proteins to Their Cellular Pathways; vitro Protein Translation Reveals a Biosignature of Robert Lj Graham; Michael J Sweredoski; Sonja Hess; Inhibitory Cold-Shock Proteins; Sushmita Mimi Roy; CalTech, Pasadena, CA Sunil Bajad; Evan Green; Junhao Yang; Tyler H WP 275 An Integrated Proteomic Approach to Decipher Cell Heibeck; James Zawada; Henry Heinsohn; Sutro Type-Specific Proteasome Structures and Functions; Biopharma Inc, South San Francisco, CA Charity Aiken1, 2; Lan Huang1, 2; 1Dept. of Physiology WP 285 Proteomic Profiling of a Wild-Type and a and Biophysics, University of California, Irvine, Irvine, Translation Defective Strain in Escherichia coli for CA; 2Dept. of Developmental and Cell Biology, Understanding Regulatory Mechanisms in Protein University of California Irvine, Irvine, CA Synthesis; Debnath Pal; Umesh Varshney; Arnab Roy; WP 276 Localization of Organelle Proteins by Isotope Pratip Saha; Goutam Kar; Aluri Srinivas; Indian Institute Tagging (LOPIT) in the HEK293T Cell Line; Andrew of Science, Bangalore, India Christoforou1; Matthew Trotter2; Phil Charles1; Alfonso WP 286 Integrating Metagenomics and Metaproteomics Martinez Arias3; Kathryn Lilley1; 1Cambridge Centre for Provides Insight into the Molecular Activities and Proteomics, Cambridge, UK; 2Laboratory for Biosignature(s) of the Human Gut Microbiota in Regenerative Medicine, Cambridge, UK; 3Department of Crohn’s Disease; Alison Russell1, 2; Nathan C. Genetics, University of Cambridge, Cambridge, UK Verberkmoes2; Manesh Shah2; Brandi Cantarel3; WP 277 A Comprehensive Strategy to Maximize the Analysis Emmanuel Mongodin3; William Hsiao3; Regina of Substrates of the Ubiquitin Proteasome Pathway Lamendella4; Claire Fraser-Liggett3; Janet Jansson4; by LC-MS/MS; Geoffrey Smith; Michael J Sweredoski; Robert Hettich2; 1University of Tennessee-Knoxville, Sonja Hess; Caltech, Pasadena, CA Knoxville, TN; 2Oak Ridge National Lab, Oak Ridge, WP 278 LC/MSE Label-Free Quantitative Proteomic Analysis TN; 3University of Maryland School of Medicine, of Purified Invasive Versus Non-Invasive Plasmodium Baltimore, MD; 4Lawrence Berkeley National Lab, falciparum Merozoites in Support Of Malaria Berkeley, CA Vaccine Development; Krishan Kumar1; Michael WP 287 Characterization of Bacterial Proteins from Nold2; J. David Haynes3, 4; Prakash Srinivasan4; J. Organisms without Sequenced Genomes; Colin Kathleen Moch3; Karine Reiter1; David Narum1; Wynne1; Nathan J. Edwards 2; Catherine Fenselau 1; 1Laboratory of Malaria Immunol and Vaccinology, NIH, 1University of Maryland, College Park, MD; Rockville, MD; 2Waters Corporation, Milford, MA; 2Georgetown University Medical Center, Washington, 3Walter Reed Army Institute of Research, Silver Spring, DC MD; 4Laboratory of Malaria and Vector Research, NIH, WP 288 Integrative Proteomics and Network Analyses of Rockville, MD Growth Stresses Induced Systems Fluctuations in 58th ASMS Conference on Mass Spectrometry Page S105 WEDNESDAY POSTERS Haloarcula Marismortui; Lichieh Julie Chu1; Hanying Comparing Cellulose and Chitin-Based Stationary Yang1; Peiyin Shih1; Yuchieh Kao1; Yihsuan Shannon Phases; Ed Bodnar; Helene Perreault; University of Tsai2; Jinzhi Chen2; Gueitang Huang1; Rueyhung Roc Manitoba, Winnipeg, Canada Weng1; Ying Sonia Ting2; Xuefeng Fung3; Wailap WP 300 Influence of Matrix on the In Source Decay of Victor Ng1; David R. Goodlett2; 1National Yang Ming Permethylated Glycans during MALDI-TOF University, Taipei, Taiwan; 2University of Washington, Analysis; Nicolas Smargiasso; Edwin De Pauw; Seattle, WA; 3Zhejiang University, Hangzhou, China University of Liege, Liege, Belgium WP 289 Toward a Complete Map of the Drosophila WP 301 High Sensitivity MALDI Analysis of Glycans by a Melanogaster Protein Interactome; Bo Zhai1; Julian New 3AQ Labeling Method in 3AQ/CHCA Liquid Mintseris1; Robert Obar1; Guruharsha Kuthethur1; Jean- Matrix; Kaoru Kaneshiro; Yuko Fukuyama; Shinichi François Rual1; Joseph Carlson2; Odise Cenaj1; Xiao Iwamoto; Sadanori Sekiya; Kenichi Taniguchi; Koichi Chen2; Aijaz Noor3; Venkateswara Onteddu3; Pujita Tanaka; Shimadzu Corporation, Kyoto, Japan Vaidya1; Kenneth Wan2; Charles Yu2; K. WP 302 Mid-Infrared 6ȝm MALDI MS of Acidic VijayRaghavan3; Susan Celniker2; Spyros Artavanis- Biomolecules; Yoshinao Wada; Michiko Tajiri; Osaka Tsakonas1; Steven Gygi1; 1Harvard Medical School, MCHRI & Osaka University, Osaka, Japan Boston, MA; 2Lawrence Berkeley National Laboratory, WP 303 High-Throughput Quantitative Analysis of Sialylated Berkeley, CA; 3Tata Institute of Fundamental Research, N-glycans Using Negative MALDI-TOF MS; Chong- Bangalore, India Feng Xu; Li Zang; Samnang Tep; Yelena Lyubarskaya; WP 290 Effects of Growth Conditions on the Secretome of Biogen Idec, Cambridge, MA Chlamydomonas reinhardtii; Lauren Fields; Casey WP 304 Negative-Ion MALDI-MSn for Discrimination of Madinger; Michelle Cushing; Jack S. Benner; New Į2,3- and Į2,6-sialylation on Glycopeptides Labeled England Biolabs, Ipswich, MA with a Pyrene Derivative; Takashi Nishikaze; Toshio WP 291 Proteomics of Leaf Tissues from Populus; Gregory Nakamura; Hiroshi Jinmei; Junko Amano; The Noguchi Hurst; Xiaohan Yang; Timothy Tschaplinski; Gerald Institute, Tokyo, Japan Tuskan; Trish Lankford; Manesh Shah; Sara Jawdy; Lee WP 305 Mass-Spectral Investigation of Sialic-acid Linkages in Gunter; Nancy Engle; Oak Ridge National Laboratory, Blood-serum Glycoproteins; William R. Alley; Milos Oak Ridge, TN V. Novotny; Indiana University, Bloomington, IN WP 292 Deep Proteome Characterization and Comparison of WP 306 Ionic liquid for Ionization Efficiency Improvement of Populus Species under Wild-Type vs. Tensional Polysaccharides in ESI-MS; Yu-Ling Chang; Chung- Stress Environmental Conditions; Paul Abraham1, 2; Hsuan Chen; The Genomics Research Center, Academia Udaya Kalluri1; Rachel Adams1, 2; Gerry Tuskan1; Bob Sinica, Taipei, Taiwan Hettich1; 1Oak Ridge National Lab, Oak Ridge, TN; WP 307 The Interplay of Permanent Charge and 2GST, University of Tennessee, Knoxville, TN Hydrophobicity with Respect to Electrospray WP 293 Coupling Sequence Identities to Exact Masses Using Ionization of Glycans; Hunter Walker; Brian Papas; a Simple, Automated Procedure; Noam Kirshenbaum; Daniel Comins; David C. Muddiman; North Carolina Michal Sharon; The Weizmann Institute of Science, State University, Raleigh, NC Rehovot, Israel WP 308 Improvement of ESI Behavior of Heparan Sulfate CARBOHYDRATES I, 294 - 319 Oligosaccharides by Using a Chip-based Pulsed Post- WP 294 Methods for Biodiscovery of Oral Glycosylation Column Makeup Flow for Online LC/MS; Yu Huang1; Xiaofeng Shi 1; Hongfeng Yin2; Kevin Killeen3; Using Liquid Chromatography Mass Spectrometry; 4 1 Liaqat Ali; Gothenburg University, Gothenburg, Sweden Joseph Zaia ; Boston University School of Medicine, Boston, MA; 2Agilent Labs, Santa Clara, CA; 3Agilent WP 295 Preparative PAGE Fractionation and ESI-FTMS 4 Analysis of Bikunin Glycosaminoglycan; Tatiana Laboratories, Santa Clara, CA; Boston University, Laremore1; Kemal Solakyildirim1; Mellisa Ly1; Franklin Boston, MA E. Leach III2; Toshihiko Toida3; Dmitri Zagorevski1; Jon WP 309 Use of Ion Mobility and Negative Ion CID Mass Amster2; Robert Linhardt1; 1Rensselaer Polytechnic Spectrometry for the Identification of N-glycans from 2 Nanomolar Amounts of Glycoprotein; David J. Institute, Troy, NY; University of Georgia, Athens, GA; 1 1 1 3Chiba University, School of Pharmacy, Chiba, Japan Harvey ; Max Crispin ; Christopher Scanlan ; Camille Bonomelli1; Frank Sobott2; Charlotte Scarff3; WP 296 Rapid Deglycosylation of Proteins for Carbohydrate 3 3 1 and Protein Characterization by Mass Spectrometry Konstantinos Thalassinos ; James Scrivens ; University of Oxford, Oxford, UK; 2CeProMa, University of Using EndoH-Bound Magnetic Beads; Casey 3 Madinger; Ellen Guthrie; Jack S. Benner; New England Antwerp, Antwerp, Belgium; University of Warwick, BioLabs, Ipswich, MA Coventry, UK WP 297 A Unique Workflow for Glycoprotein WP 310 A Separation-Free Quantitative MS-Based Profiling Characterization from Sample Preparation to Approach Using 2-AA Isotopically Labeled MS/MS Spectral Interpretation; Jenny Albanese1; Substrates for High-Throughput Glycan Screening; Randy Lee2; 1Applied Biosystems part of Life Justin M Prien; Lorna Maheu; Brad Prater; Qiang Qin; Technology, Foster City, CA; 2ProZyme, Inc., Hayward, Steve Cockrill; Amgen, Longmont, CO CA WP 311 LC/MS Analysis of Heparan Sulfate Chain WP 298 2D-HPLC with Online and Offline Orbitrap Mass Processing by Human Sulf2; Gregory O Staples; Spectrometry for Profiling and Characterization of Xiaofeng Shi; Joseph Zaia; Boston University School of N-linked Glycans Released from Biotherapeutic Medicine, Boston, MA Receptor-Fc Glycoproteins; Andrew Hanneman; Chi- WP 312 Characterization of Heparan Sulfate N-sulfated Ting Huang; Acceleron Pharma, Cambridge, MA Domains Binding Fibroblast Growth Factor-2.; WP 299 An Investigation to Assess the Enrichment of Hicham Naimy; Nancy Leymarie; Joseph Zaia; Boston Glycopeptides from Tryptic Digestion Mixtures by Univ., Boston, MA Page S106 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS WP 313 A Novel Strategy for Characterization of Mucins WP 325 Integrating Shotgun Lipidomics and HPLC Toward Biomarker Discovery: Composition-Based Separation of Lipids into a New Platform for Total Arrangement of Mass Spectral Data; Yu-Ki Matsuno1; Lipid Analysis; Reinaldo Almeida1; Christer Ejsing2; Weijie Dong1; Seiya Yokoyama2; Suguru Yonezawa2; 1Advion BioSciences Ltd., Arnsberg, Germany; Hisashi Narimatsu1; Akihiko Kameyama1; 1Research 2University of Southern Denmark, Odense, Denmark Center for Medical Glycoscience, AIST, Tsukuba, Japan; WP 326 Distinctive Oxidized Cardiolipin Species Generated 2Human Pathology, Kagoshima University, Kagoshima, by Singlet Oxygen and Radical Initiator Japan Characterized by HPLC-MS/MS Analysis; Junhwan WP 314 Novel Sulfated and Fucosylated Glycans Isolated Kim; Paul Minkler; Robert Salomon; Charles Hoppel; from Human Saliva and Tear; Sureyya Ozcan; Hyun Case Western Reserve University, Cleveland, OH Joo An; Carlito Lebrilla; Department of Chemistry, WP 327 The Identification and Quantification of Molecular University of California, Davis, CA Lipid Species by Higher Energy Collisional WP 315 Mass Spectrometric Analysis of N-linked Glycans Dissociation on Orbitrap Instruments.; Kai from Folate Receptor; Nidhi Jaiswal; Suraj Saraswat; Schuhmann1, 2; Ronny Herzog2; Stefan R. Bornstein1; Mahohar Ratnam; Dragan Isailovic; University of Andrej Shevchenko2; 1Department of Internal Medicine Toledo, Toledo, OH III, TU Dresden, Dresden, Germany; 2Max Planck WP 316 Site-Specific N-Glycosylation of Biologically Active Institute CBG, Dresden, Germany L-Selectin; Véronique Blanchard; Stefanie Wedepohl; WP 328 Characterization of Nonpolar Lipids by Using Laser- Matthias Kaup; Sebastian Riese; Jens Dernedde; Markus Induced Acoustic Desorption/Chemical Ionization, Berger; Rudolf Tauber; Charité Berlin, Berlin, Germany APCI, and ESI; Zhicheng Jin; Shavani Daiya; Hilkka WP 317 Characterization of Chondroitin Sulfate Using High Kenttamaa; Purdue University, West Lafayette, IN Resolution Ion Trap-Time of Flight (IT-TOF) Mass WP 329 Analysis of a New Immunomodulatory Milk Lipid Spectrometry; Hiro Katagiri1; Herbert M. Geller1; Faith Particle Using Top-Down Lipidomics on a Hybrid A. Hays2; Yuhui Wang2; William A. Hedgepeth2; Triple Quadrupole FT-ICR Mass Spectrometer; Masatoshi Takahashi2; 1National Heart, Lung, and Larry Lerno1; Jennifer T. Smilowitz2; J. Bruce German2; Blood Institute, NIH, Bethesda, MD; 2Shimadzu Carlito Lebrilla1; 1UC Davis, Dept. of Chemistry, Davis, Scientific, Columbia, MD California; 2UC Davis, Dept. of Food Science, Davis, WP 318 N-Glycan Profile Comparison of Serum and Cancer California Cell Culture Lysate Using Liquid Chromatography WP 330 Lipid Analysis from Biological Samples by Normal- Mass Spectrometry; Kathryn Cindric; Ting Ye; Kinjal Phase Chromatography / Atmospheric Pressure Amin; Qinfeng Liu; Campbell University, Buies Creek, Photoionisation Mass Spectrometry.; Mathieu NC Gaudin1, 4; Laurent Imbert2; Danielle Libong2; Pierre WP 319 A Mass Spectrometry Based Glycomic Approach for Chaminade2; Philippe Loiseau3; Julie Allegrand4; Auzeil Identification of Carbohydrate Dependant Virulence Nicolas1; David Touboul4; Olivier Laprévot1,4; 1C-TAC, Factors Using Caenorhabditis Elegans as a Surrogate Descartes University, Paris, France; 2Paris-Sud Host; Md Mizanur Rahman 1; Jonathan Hodgkin2; John Analytical Chemistry Group, Chatenay-Malabry, F Cipollo1; 1Food and Drug Administration/ CBER, France; 3BIOCIS, Paris-Sud Faculty of Pharmacy, Bethesda, MD; 2Departmnent of Biochemistry, Chatenay-Malabry, France; 4CNRS-ICSN, Gif-Sur- University of Oxford, Oxford, UK Yvette, France LIPIDS II, 320 - 340 WP 331 Reversed Phase Liquid Chromatography Coupled to ESI Mass Spectrometry for Separation, Detection of WP 320 Comprehensive Lipid Profiling of Algal Strains for 1 Biofuel Feedstock by High Resolution Mass Polar Lipids in Biological Samples; Ying Zhou ; Julian Ollesch2; Holger Wille2; David S. Newburg3; Catherine Spectrometry; Karen Glenn; Patrick McGinn; Stephen 1 1 O'Leary; Jeremy Melanson; National Research Council E. Costello ; Boston University School of Medicine, Boston, MA; 2University of California, San Francisco, of Canada, Halifax, Canada 3 WP 321 Lipidomics: Fatty Acid and Lipid Profiling in Human San Francisco, CA; Harvard University, Massachusetts Plasma Using RP-HPLC/ESI-MS Method; Miroslav General Hospital, Boston, MA Lísa; Eva ýáĖová; KateĜina Netušilová; Michal WP 332 Identification of Triacylglycerol in Oils Using LTQ- Orbitrap Mass Spectrometer; CheongTae Kim2; Holþapek; University of Pardubice, Pardubice, Czech 2 2 1 1 Republic DaeHyun Kim ; WooSeok Kang ; Jongsoo Park ; Euro Science Co., Ltd., Sungnam City, South Korea; WP 322 Different Distribution of Individual Molecular 2 Species in Mouse Organs Obtained by Automated NongShim Co., Ltd, Seoul, South Korea Lipid Search Engine and Multivariate Analyses; Ryo WP 333 Rapid Liquid Chromatography-Mass Spectrometry Taguchi; Hiroki Nakanishi; Moonjin Ra; The University Screening for Lipids in the Unicellular Red Alga of Tokyo, Tokyo, Japan Porphyridium cruentum; Behnaz Shafii; A. Daniel WP 323 Triacylglycerol Profiling and Positional Isomer Jones; Michigan State University, East Lansing, MI Analysis in Omega-3 Enriched Fish Oil by Mass WP 334 Nascent HDL from ABCA1: Using the Lipid Spectrometry; Lisandra Cubero Herrera1; Karen M. Composition of Nascent HDL to Deduce the Glenn1; Tobias Karakach1; Michael A. Potvin2; Jeremy Intracellular Lipid Source; Shaila Bhat; Mary G. E. Melanson1; 1NRC Institute for Marine Biosciences, Sorci-Thomas; John S. Owen; Dharika Shah; Michael P. Halifax, Canada; 2Ocean Nutrition Canada, Dartmouth, Samuel; Manal Zabalawi; Michael J. Thomas; WFU Canada School of Medicine, Winston-Salem, NC WP 324 Lipidomics and Neurodegeneration; Sarita Hebbar; WP 335 NP-HPLC/MS/MS Analysis of Neutral Lipid Oxidation in Human Atherosclerosis; Patrick Dominik Schwudke; National Centre for Biological 1 2 1 Sciences, TIFR, Bangalore / Bengaluru, India Hutchins ; Robert C. Murphy ; Univ of Colorado, Denver, CO; 2University of Colorado Den, Aurora, CO 58th ASMS Conference on Mass Spectrometry Page S107 WEDNESDAY POSTERS WP 336 HPTLC-MALDI MS and MS/MS Analysis of Lipid White1; 1NIST, Gaithersburg, MD; 2Liege University, Mixtures; Martin Schuerenberg1; Beate Fuchs2; Andrea Liège, Belgium Bischoff2; Rosemarie Suess2; Detlev Suckau1; Ulrike WP 347 Metabolomic Analysis Reveals Metabolic Anders1; Gertrud Morlock3; Juergen Schiller2; 1Bruker Conditionality of Antibiotic Resistance in Daltonics, Bremen, Germany; 2University Leipzig, Staphylococcus aureus; Steven M. Fischer1; Elizabeth Medical School, Leipzig, Germany; 3University of Alexander2; Kyu Rhee2; 1Agilent Technologies, Santa Hohenheim, Inst. of Food Chemistry, Stuttgart, Germany Clara, CA; 2Cornell Medical College, New York, NY WP 337 A Novel Method for Analysis of Diversities in WP 348 Combined use of HILIC and RPLC Increases Ceramides and Glycosphingolipids by MALDI- Metabolite Coverage in LC-MS Analyses of Yeast TOFMS and MS2 Equipped with High-Energy CID; Metabolome; Anna Lindahl; Hasanuzzaman Bhuiyan; Kouji Tanaka1, 2; Masaki Yamada3; Yuzo Yamazaki3; Anders Nordstrom; Karolinska Institute, Stockholm, Andrew Eaton4; Toshifumi Aoyama5; Atsushi Hara5; Sweden Reiji Kannagi1; Mamoru Kyogashima1, 2; 1Aichi Cancer WP 349 Metabolomic Profile of Human Serum to Determine Center Research Institute, Nagoya, Japan; 2Nagoya City the Age Related Immunosenescence Biomarkers; University, Nagoya, Japan; 3Shimadzu Corporation, Gauthier Eppe; Andrei Turtoi; Edwin De Pauw; Liege Kyoto, Japan; 4Kratos Analytical, Manchester, UK; University, Liège, Belgium 5Shinshu University, Graduate School of Medicine, WP 350 GCxGC-TOFMS Elucidation of Differences in the Matsumoto, Japan Metabolome of Strains of the Pathogen Stagonospora WP 338 Regioisomeric Characterization of Triacylglycerols nodorum; Robert Trengove 1, 3; Joel Gummer2; Using Atmospheric Pressure Chemical Ionization Catherine Rawlinson3; Garth Maker1; Christian Krill2; Mass Spectrometry and Silver-Ion Liquid Peter Solomon4; Richard Oliver2; 1Murdoch University, Chromatography; Michal Holcapek; Hana Velinska; Murdoch, Australia; 2ACNFP, Murdoch University, Miroslav Lisa; University of Pardubice, Pardubice, Murdoch, Australia; 3Metabolomics Australia, Murdoch Czech Republic University, Murdoch, Australia; 4The Australian WP 339 Ozone Induced Dissociation for Lipidomics National University, Canberra, Australia Workflows; Berwyck Poad1; Huong Pham Thu3; Todd WP 351 LC/MS Based Metabolomics Approach for the Study W Mitchell1; J. Larry Campbell2; Stephen J Blanksby1; of Salicylic Acid Response in Arabidopsis thaliana; 1University of Wollongong, Wollongong, Nsw, Australia; Jong Bok Seo1; Kwang Chul Kwon1; Hak Chul Jin2; Yu 2MDS Analytical Tech, Sciex, Concord, ON; 3School of Ran Kim1; Ohkmae K Park2; Myung Hee Nam1; 1Korea Chemistry, University of Wollongong, Wollongong, Basic Science Institute, Seoul, South Korea; 2Korea Australia University, Seoul, South Korea WP 340 Double Bond Localization in Wax Ester Molecular WP 352 A Rapid Method for Estimating Triglyceride Species by HPLC/APCI-MS Utilizing CID of the Synthesis in vivo Using Stable Labeled Fatty Acids Acetonitrile Adducts; Vladimir Vrkoslav; Martina and Analysis by UPLC-MS/MS; Timothy He; David Háková; Josef Cvaþka; Institute of Organic Chemistry G. McLaren; Jose Castro-Perez; Stephen Previs; Sheng- and Biochemistry, Prague, Czech Republic ping Wang; Ray Rosa; Vivienne Mendoza; Douglas G. METABOLOMICS III, 341 - 367 Johns; Thomas P. Roddy; Merck & Co., Rahway, NJ WP 341 Metabonomic Approaches for Identification of WP 353 Comparison of Metabolomic Profiles between the Urinary Phospholipids as Markers of Drug-Induced Androgen-Dependent and -Independent Prostate Cell Lines by Using Liquid Chromatography and Mass Phospholipidosis; Petia Shipkova; Serhiy Hnatyshyn; 1 Nelly Aranibar; Michael Reily; Monicah Otieno; Lois Spectrometry (LC-MS); Nagireddy Putluri ; Shaiju Vareed1; Theodore Sana2; Steven Fischer2; Gagan Lehman-McKeeman; Don Robertson; Bristol-Myers 1 1 3 4 Squibb, Princeton, NJ Thangjam ; Vasanta Putluri ; Ling Lan ; Judith Giri ; Arun Sreekumar1; 1Cancer Center, Medical College of WP 342 Characterising Functionalised Surfaces for Studying 2 Metabolite-Protein Interaction, Using ToF-SIMS; Georgia, Augusta, GA; Metabolomics Laboratory Malinda Salim; Phillip C Wright; Seetharaman Application Group, Agilent Technologies, Santa Clara, CA; 3Department of Biostatistics, Medical College of Vaidyanathan; The University of Sheffield, Sheffield, UK 4 WP 343 Discovery of Cancer Stem Cell Biomarkers Using Georgia, Augusta, GA; Pathology, Biomedical and Metabolomics; Paul R. West; Alan Smith; April Weir; Radiological Technologies, Medical College of Georgia, Gabriela Cezar; Stemina Biomarker Discovery, Madison, Augusta, GA WI WP 354 Searching the Hydrophobic Cargo of Human Serum WP 344 Metabolomics and Integrated Functional Genomics Albumin to Gain Insights into Type II Diabetes; Jared Bowden1; Penggao Duan2; Edward Dratz1; 1Montana for the Discovery and Elucidation of Saponin 2 Biosynthesis; Lloyd W. Sumner; John H. Snyder; David State University, Bozeman, Montana; Bruker Daltonics, V. Huhman; Stacy Allen; Yuhong Tang; The Noble Billerica, MA WP 355 Changes in the Serum Metabolome Caused by Foundation, Ardmore, OK 1 WP 345 Targeted and Non-Targeted Profiling and Inflammation; Erin G. Prestwich ; Ramesh Babu Indrakanti2; Koli Taghizadeh1; Peter Dedon3; Quantification of Plant Hormones and Related 1 2 1 Massachusetts Institute of Tech, Cambridge, MA; MIT, Compounds by Mass Spectrometry; Baichen Zhang ; 3 Leslie M. Hicks2; 1Donald Danforth Center, St Louis, Cambridge, MA; MIT, Dept. of Biological Engineering, MO; 2Danforth Center, St. Louis, MO Cambridge, MA WP 346 Identification of Derivatized Components of WP 356 Flux Analysis of Central Metabolic Pathways in Thermoanerobacter Tengcongensis; Wei Tong1; Qian Standard Reference Material (SRM) 1950 1 3 1 1 Metabolites in Human Plasma by Linear Quadrupole Wang ; Zhe Cao ; Zhuowei Wang ; Zhen Chen ; Chuanqi Zhou1; Quanhui Wang1; Rong Wang1, 2; Siqi Gas Chromatography/Mass Spectrometry.; Bruce A. 1 1 Benner1; Gauthier Eppe2; Nathan G. Dodder 1; Edward Liu ; Beijing Institute of Genomics, CAS, Beijing, Page S108 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS China; 2Mount Sinai School of Medicine, New York, NY; Spectrometry; Catharina Crone1; Michaela Scigelova1; 3Agilent Technologies Co. (China), Beijing, China Olaf Scheibner1; Martin Hornshaw2; Luis Duarte3; Luísa WP 357 Improving the Performance of AccQ•Tag Amino B. Roseiro3; Francisco Girio3; M.Gabriela Bernardo- Acid Quantitation in Biological Samples by UPLC- Gil4; 1Thermo Fisher Scientific (Bremen), Bremen, ESI-MS/MS Using the Xevo TQ Mass Spectrometer; Germany; 2Thermo Fisher Scientific (Hemel Carolina Salazar1; Jenny M. Armenta2; Diego F. Cortes Hempstead), Hemel Hempstead, UK; 3Laboratório 1; Vladimir Shulaev1; 1Virginia Bioinformatics Inst., Nacional de Energia e Geologia, Lisboa, Portugal; Blacksburg, VA; 2Waters Corporation, Beverley, MA 4Centro de Engenharia Química e Biologica, Lisboa, WP 358 Cigarette Smoke Exposure Induces Metabolome Portugal Alterations in Uterine Tissue of Pregnant Mice; SMALL MOLECULE ANALYSIS, 368 - 399 1 1 2 Rachel Neal ; Jing Chen ; Robert M. Greene ; M. WP 368 Withdrawn Michele Pisano2; 1EOHS, SPHIS, University of 2 WP 369 The Analysis of Free and Total Amino Acids in Food Louisville, Louisville, KY; Birth Defects Center, Matrices Using aTRAQ Technology Chemistry and University of Louisville, Louisville, KY LC/MS/MS Specificity; Jim Krol; Scott B. Daniels; WP 359 Metabolomics of Hypobaric Hypoxia: Oxidative Babu Purkayastha; Applied Biosystems, Framingham, Stress and Its Metabolic Signature; Denise U. 1 2 3 MA Sonntag ; Jacqueline Pichler Hefti ; Urs Hefti ; Lorenz WP 370 2-Methyl-2-nitrosopropane, A Promising Label for Risch4; Tobias M. Merz5; Andreas Huber2; Klaus M. 1 1 Biological Thiol Targets: A Combined Electronic Weinberger ; BIOCRATES Life Sciences AG, Paramagnetic Resonance and Electrospray Tandem Innsbruck, Austria; 2Center of Laboratory Medicine, 3 Mass Spectrometry Study.; Mathilde Triquigneaux; Kantonsspital, Aarau, Switzerland; Department of Beatrice Tuccio; Laurence Charles; laboratoire chimie Orthopedic Surgery, Kantonsspital, Liestal, Switzerland; 4 5 provence, Marseille, France VIVIT-Institute, Feldkirch, Austria; Dept of Intensive WP 371 The Characterization and Differentiation of Sorbitan Care Med., University Hospital, Bern Switzerland Esters by LC-MS-MS Combined with Statistical Data WP 360 Evaluation of the LTQ-Orbitrap Technology for Processing.; Stephen Rumbelow1; Johnie Brown2; Quantitative Metabolite Profiling in Cell Extracts; 1 2 1 2 1 Croda Inc, New Castle, DE; Applied Biosystems, Ludovic Muller ; Jean Labarre ; Sandra Alves ; Eric Framingham, MA Ezan3; Jean-Claude Tabet1; Christophe Junot3; 1IPCM, 2 WP 372 LC/MS/MS Approach to Determine in vivo Receptor UPMC Paris Universitas, Paris, France; DSV/iBiTec- Occupancy of CNS Drugs at GABAA and D2 S/SBIGeM, CEA/Saclay, Gif-sur-Yvette, France; 3 Receptors in Rat Brain; Farzin Gharahdaghi; John DSV/iBiTec-S/SPI, CEA/Saclay, Gif-sur-Yvette, France Zysk; Teng Peng; John Roberts; Reto Gadient; Vijay WP 361 Mass Spectrometric Detection of Nucleotides in Chhajlani; Min Ding; Donna Maier; Astrazeneca, Single Muscle Cells and Development of an LDI- Wilmington, DE Based Signal Amplification Assay; Stephan Rupert WP 373 Comparison of LC-MS, LC-CAD and Indirect CE as Fagerer1; Pawel Lukasz Urban1; Fabian Dettwiler1; 1 1 1 the Assay Technique for a Non-Chromophoric David Aguayo ; Claudio Signorell ; Jachen Denoth ; Impurity in an Active Pharmaceutical Ingredient; Yu-Chie Chen2; Renato Zenobi1; 1ETH Zurich, Zurich, 2 Hong Gao; Merck & Co. Inc., Rahway, NJ Switzerland; NCTU, Hsinchu, Taiwan WP 374 The Odds are in Your Favour: An Investigation of WP 362 Two pH Optimized LC-MS Methods for the Unusual Fragmentations of Quinazolines Using Metabolomics Analysis of Hydrophilic Compounds ESI-QIT-MS and DFT Calculations.; Angelika on Silica Hydride Stationary Phase; Theodore R. Sana 1 2 1 1 1 1 2 Galezowska ; Mark W. Harrison ; G. John Langley ; ; Steven M. Fischer ; Stefan Jenkins ; Maria Matyska ; 1University of Southampton, Southampton, UK; Joseph J. Pesek3; 1Agilent Technologies, Santa Clara, 2 2 3 Analytical Chemistry Group, AstraZeneca, CA; MicroSolv Technologies, Eatontown, NJ; San Jose Macclesfield, UK State University, San Jose, CA WP 375 Pesticide Screening and Quantitation in Cotton WP 363 Isotopomics: A Tool for Mapping Dynamic Goods; Anna Marques1; April L. Thomas1; Jose Costa2; Metabolism in Rats; Jean-Philippe Godin; Nestlé Daniel Lebre3; Andre Schreiber4; 1AB Sciex USA, Research Center, Lausanne, Switzerland Framingham, MA; 2AB Sciex Brazil, Sao Paulo, Brazil; WP 364 Plant Metabolomics: Tomato Metabolite Profiling 3CEMSA, Sao Paulo, Brazil; 4AB Sciex Canada, and Identification Employing High Resolution MS 1 2 Concord, Canada Strategies; Helen Welchman ; David Portwood ; Mark WP 376 Quantitation of Nitroglycerin, Glyceryl 1,2-dinitrate Seymour2; Mark Earll2; Gary Woffendin1; Martin 1 3 1 and Glyceryl 1,3-dinitrate in Human Plasma via Hornshaw ; Michael Athanas ; Madalina Oppermann ; HPLC with MS/MS Detection; Paul Connolly; 1Thermo Fisher Scientific, Hemel Hempstead, UK; 2 3 Jingduan Chi; Jennifer Ammarman; Denise Hemminger; Syngenta, Bracknell, UK; VAST Scientific, Boston, MA Emily Barrey; MPI Research Inc., State College, PA WP 365 LC-MS Analysis of Hydrophilic Metabolites in WP 377 Evaluation of Accurate Mass and Dynamic Range Guinea Pig Lungs Infected with Mycobacterium Capabilities of Low and High-Resolution Tuberculosis; Justin Searcy; Gavin Ryan; Courtney Instrumentation in Compound Identification in Drug Hastings; Dean Crick; Colorado State University, Fort Discovery.; Vladimir Capka1, 2; Sharon Tentarelli1, 2; Collins, CO 1Astra Zeneca R&D Boston, Waltham, MA; 2Astra WP 366 A Differential Isotope-Coded Mass Defect Label for Zeneca R&D Boston, Waltham, MA Metabolite Discovery and Relative Quantitation; WP 378 When Stability, Solubility, and Chiral Selectivity Jacobo Iglesias; Albert Koulman; Dietrich Volmer; Intertwine: A Quantitative Assay for Medical Research Council, Cambridge, UK Dexlansoprazole; Ryan S. Adler; Brad Bessette; Min WP 367 Antioxidants in Carob Bean Extracts: Evaluation of Meng; Spencer Carter; Scott A. Reuschel; Patrick Extraction Methods Using High Resolution Mass Bennett; Tandem Labs, Salt Lake City, UT 58th ASMS Conference on Mass Spectrometry Page S109 WEDNESDAY POSTERS WP 379 Characterization of Potentially Bioactive Peptides WP 389 Fragmentation Pathway Study of Rhein by Ion Trap and Small Molecules in Tetraponera rufonigra Using Combined Time-of-Flight Mass Spectrometer.; Jing HPLC-Chip Cube - Accurate-Mass Q-TOF MS and Dong; Lei Cao; Shimadzu International Trading MS/MS; Hooi Ling Liew1; Aishah A. Latiff1; Michael (Shanghai) Co., Limit, Beijing, China Harvey2; Chow-Yang Lee3; Chee Yuen Gan1; 1Doping WP 390 Gas Chromatography-Atmospheric Pressure Control Centre, Minden, Malaysia; 2Oasis Biologics Sdn Chemical Ionisation Time of Flight Mass Bhd, Damansara Perdana, Malaysia; 3School of Spectrometry Optimisation and Application to Biological Sciences, USM, Minden, Malaysia Impurity Identification in Drug Development; Mark WP 380 The SALDÎMS Screening and LĈMS/MS W. Harrison1, 1; Anthony W.T. Bristow1, 2; Martin Sims1, 2 1, 3 1, 3 1 Identification of Amino Acids and Amines from the ; Athen-Engeland Thomas ; Barsch Aiko ; , Miller Volcanic Spark Discharge Experiment; Zdenek Macclesfield, UK; 2AstraZeneca, Macclesfield, UK; Spacil1, 2; Ly Jiang3; Pawel Dziedzic3; Frank Turecek1; 3Bruker Daltonics GmbH, Bremen, Germany Armando Cordova3, 4; 1University of Washington, Seatte, WP 391 Detection and Identification by LC-MS of an WA; 2Charles University in Prague, Hradec Kralove, Extractable/Leachable Impurity Essential for Czech Republic; 3Stockholm University, Stockholm, Ophthalmic Formulation Stability.; Audrey Sweden; 4Mid Sweden University, Sundsvall, Sweden Tousignant; Yvonne Lear; Éric Vincent; Anne Danion; WP 381 Verification of the Origin of Whisky by Electrospray Alain Carrier; Sandoz Canada Inc., Boucherville, FT-ICR Mass Spectrometry and Statistical Methods; Canada Matthias Witt 1; Rainer Paape1; Jens Fuchser1; Gary WP 392 Analysis of the Formation of Novel Melphalan Kruppa2; 1Bruker Daltonik GmbH, Bremen, Germany; Hydrolysis Products under Isolated Lung Perfusion 2Bruker Daltonics Inc., New York, NY Conditions Using LC-MSMS; Jasper Boschmans1; WP 382 Analysis of Black Tea Thearubigins: An Willem den Hengst4; Paul Van Schil4; Ernst de Bruijn3; Electrospray-FT-ICR Mass Spectrometric Study; Filip Lemière1; Erwin Witters 1, 2; 1University of Matthias Witt 1; Nikolai Kuhnert2; Michael Clifford3; M Antwerp, Antwerp, Belgium; 2Flemish Institute for Gokhan Baykut1; 1Bruker Daltonik GmbH, Bremen, Technological Research, Antwerp, Belgium; 3KULeuven, Germany; 2Jabocs University, Bremen, Germany; Leuven, Belgium; 4University Hospital Antwerp, 3University of Surrey, Guilford, UK Antwerp, Belgium WP 383 Differentiation of Diastereomeric Phosphoramidate WP 393 Identification and Confirmation of an Unknown Prodrugs with Electrospray LC-MS; Donghui Bao; P. Degradation Product in the Stability Samples of a Ganapati Reddy; Bruce Ross; Michael J. Sofia; Pharmaceutical Dosage Form; Charles Pan 1; Jack Pharmasset, Princeton, NJ Guan2; Melissa Lin1; 1Novartis, East Hanover, NJ; WP 384 TLC-MS/MS: Integrated Liquid Surface Sampling- 2Sandoz, East Hanover, NJ Nano Electrospray Determination of Small Drug WP 394 Isomer Differentiation of Hydroxypyridine N-oxides Molecules from Thin Layer Chromatography Plates.; Using Metal Complexation and Electrospray Jack D. Henion1; Daniel Eikel2; Chris Alpha; Simon J. Ionization Mass Spectrometry; Matías Butler; Pau Prosser4; Geoffrey S. Rule6; Joseph Ebel5; 1Advion Arroyo Mañez; Gabriela Cabrera; Universidad de BioSciences, Inc, Ithaca, NY; 2AdvionBioSystems, Buenos Aires, Buenos Aires, Argentina Ithaca, NY; 3Advion Biosystems Inc., Ithaca, NY; WP 395 Analysis of an Expired Opthalmic Solution of 4Advion BioSciences, Inc., Ithaca, NY; 5Analytical Polymycin B Sulfate and Trimethoprim by LC-MS; Toxicology, Cornell University, Ithaca, NY; 6Advion Sharanya Reddy; David Negrotti; Avinash Dalmia; Biosystems, Salt Lake City, UT Daniel Pentek; William Goodman; PerkinElmer, WP 385 Highly Sensitive LC/MS/MS Method for Shelton, CT Quantitation of Bronchodilators in Dried Blood Spot WP 396 Dried Blood Spot Analysis - Utilizing the Technique Samples; Suma Ramagiri1; Mauro Aiello 1; Hesham to Develop Assays in Rare or Limited Matrices; Chad Ghobarah1; Neil Spooner2; 1AB/ SCIEX, Concord, Christianson; Casey Johnson; Jennifer Zimmer; Shane Canada; 2GlaxoSmithKline Pharmaceutical, Welwyn, Needham; Alturas Analytics, Inc., Moscow, ID UK WP 397 SFC/MS and LC/MS Analysis of Bio-Diesel Fuels; WP 386 Formation and Biological Activity of 11-oxo- Anna Marques1; Debora Santos2; Rogerio C. Rodrigues3; 3 4 eicosatetraenoic Acid, a Novel 15- Alexandre S. Machado ; David J. Tognarelli ; Becky 5 6 6 6 hydroxyprostagladin Dehydrogenase-Derived Wittrig ; Robert Ellis ; Stephen Tate ; Mauro Aiello ; Arachidonic Acid Metabolite; Cong Wei; Suhong Tadeo Sakuma6; 1ABSciex USA, Framingham, MA; Zhong; Xiaojing Liu; Ian Blair; Center for Cancer 2ABSciex Brazil, Sao Paulo, Brazil; 3SENAI/CETIND, Pharmacology, University of Penn, Philadelphia, PA Lauro de Freitas, Brazil; 4JASCO, Easton, MA; 5Restek WP 387 Rapidly Screening of Melamine in Milk Using Liquid Corporation, Bellefonte; 6ABSciex Canada, Concord, Electrospray Laser Desorption / Ionization Mass CA Spectrometry; Ming-Hui Yang1; Chu-Nian Cheng1; Yu- WP 398 Adding Empirical Formula Rules to Accurate Mass Chang Tyan2; Jentaie Shiea 1; 1National Sun Yat-Sen and Exact Isotope Modeling for Elemental Univ., Kaohsiung, Taiwan; 2Kaohsiung Medical Composition Determination; Ming Gu; Yongdong University, Kaohsiung, Taiwan Wang; Cerno Bioscience, Yardley, PA WP 388 Analysis of Impurities in Į-zearalanol by High WP 399 LC-MS/MS Method Improvement Using Dried Blood Performance Liquid Chromatography/Ion Spot Technology: Evaluation of Extraction Trap/Time-of-Flight Mass Spectrometry; Kang Ma1; Efficiency, HPLC Column Life Span and Jing Dong 2; Jinjing Xing3; 1National Institute of Automation; Jie Zhang; Shimin Wei; Harold T Smith; Metrology of China, Beijing, China; 2Shimadzu Francis LS Tse; Novartis Institutes for BioMedical International Trading(Shanghai)Co., Limit, Beijing, Research, East Hanover, NJ China; 3Jiangsu Institute of Metrology, Nanjing, China Page S110 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS LC-MS SAMPLE PREPATION I, 400 - 427 Extraction and LC/MS/MS; Joelle Onorato; Luping WP 400 Cross-Validation of Supported-Liquid Extraction Chen; Petia Shipkova; Zhengping Ma; Anthony Azzara; and Solid-Phase Extraction Methods for Soy James Devenny; Ningning Liang; Tasir Haque; Dong Isoflavones in Serum and Urine Using UPLC- Cheng; Bristol-Myers Squibb, Princeton, NJ ES/MS/MS; Kellie Woodling; Nathan C. Twaddle; WP 414 Determination of Trace Sulfonamides in Swine Waste Daniel R. Doerge; NCTR/FDA, Jefferson, AR Water by Three-Phase Liquid-Phase Microextraction WP 401 Assessment of Protein Digestion Protocols by Combined with Liquid Chromatography-Tandem Combining Standard (LTQ-Orbitrap-MS) and Mass Spectrometry; Chien-Chun Shen; Chung-Yu unbiasEd (QToF-MSE) Tandem Mass Spectrometry; Chen; Maw-Rong Lee; National Chung-Hsing Ileana R. León; Richard R. Sprenger; Simone Sidoli; University, Taichung, Taiwan Clifford Young; Ole N. Jensen; Univ. of Southern WP 415 Sensitive Femtogram Determination of Aflatoxins B1, B2, G1 and G2 in Food Matrices Using Tandem LC- Denmark, Odense, Denmark 1 2 1 WP 402 Qualification of Automation Procedures for MS/MS; Yang Chen ; Peter Stone ; Jack Cappozzo ; 1National Center for Food Safety and Technology/IIT, Preparation of Calibration Standards and QCs in 2 Biological Matrices; Jie Zhang; David Ayres; Shimin Summit-Argo, IL; Agilent Technologies, Inc., Santa Wei; Harold T Smith; Francis LS Tse; Novartis Clara, CA Institutes for BioMedical Research, East Hanover, NJ WP 416 Mercury and Arsenic Speciation Analysis by IC- ICP/MS; Jörg Kleimann1; Jay Gandhi2; Laura H. WP 403 Extraction of Tamoxifen and Metabolites from Urine 3 3 1 and Plasma Using Supported Liquid Extraction Reyes ; H.M. Skip Kingston ; Metrohm International Headquarters, Herisau, Switzerland; 2Metrohm USA, (SLE) prior to LC-MS/MS Analysis; Elena Gairloch; 3 Lee Williams; Rhys Jones; Helen Lodder; Geoff Davies; Tampa, FL; Duquesne University, Pittsburgh, PA Steve Jordan; Richard Calverley; Claire Desbrow; Gary WP 417 A Highly Sensitive Method for the Quantitation of Dowthwaite; Biotage GB Limited, Cardiff, UK Testosterone and Dihydrotestosterone in Human WP 404 Mixed Mode Polymeric SPE for the Fractionation of Serum Using LC-MS/MS; Ginny B. James; Jonathan Acidic, Basic and Neutral Combination Therapeutics Rathe; Curtis Sheldon; Chad Briscoe; Ridha Nachi; MDS in Plasma; Paul Boguszewski; William Hudson; Yung- Pharma Services, Lincoln, NE lin Chen; Varian, Inc., Lake Forest, CA WP 418 Increased Protein Coverage and Tyrosine Cleavage WP 405 Extraction of Paraquat and Diquat from Urine: of Chymotrypsin In-Gel Digestions; Tatiana N. Method Optimisation Using a New Resin-based Boronina; Robert N. Cole; Johns Hopkins School of Mixed-Mode Weak Cation Exchange SPE Sorbent.; Medicine, Baltimore, MD Lee Williams; Rhys Jones; Geoff Davies; Helen Lodder; WP 419 Extraction of THC and Metabolites from Urine and Gary Dowthwaite; Claire Desbrow; Richard Calverley; Plasma Using Supported Liquid Extraction (SLE) Steve Plant; Steve Jordan; Biotage GB Limited, Cardiff, Prior to LC-MS/MS Analysis; Rhys Jones; Lee UK Williams; Helen Lodder; Geoff Davies; Steve Jordan; WP 406 Rapid and Generic Sample Clean-up of Clinical Richard Calverley; Claire Desbrow; Gary Dowthwaite; Samples for Quantitation of a Drug Candidate; S. Biotage GB Limited, Cardiff, UK Nicholas Potter; Michael Boudreaux; Janet Tam; Julie WP 420 Efficiency Assay of Detergent Removal Columns on Zalikowski; Amgen Inc., Seattle, WA Protein and Peptide Samples for Mass Spectrometric Analysis; Sophie Alvarez1; Aftab Alam2; Leslie M. WP 407 Microcrystalline Cellulose Degradation with a 1 1 2 Cellulase Enzyme Employing LC-MS to Follow Hicks ; Danforth Center, St Louis, MO; G- Glucose Formation and Degradation Impurities.; Biosciences/Genotech, Maryland Heights, MO Louis-Philippe Labranche1; Jonathan Drapeau1; Anne WP 421 The Use of a New Automated Blood Analyzer for the Danion1; Alain Carrier2; 1Sandoz, Boucherville, Canada; Determination of Immunosuppressants in Whole 2 Blood; Meike Baden1; Norbert Helle1; Fredrick D. Sandoz Canada, Boucherville, QC 2 2 3 1 WP 408 Optimizing Tissue Analysis For LC-MS/MS in Foster ; Dirk Bremer ; Juergen Wendt ; TeLA GmbH, Bremerhaven, Germany; 2Gerstel GmbH & Co KG, Support of Drug Distribution Studies; Nalini Anand; 3 Michael Koleto; Rohan Thakur; Tong Wu; Taylor Mülheim an der Ruhr, Germany; Agilent Technologies, Technology, Princeton, NJ Waldbronn, Germany WP 409 Quantification of Clindamycin in Human Plasma by WP 422 Quantification of Lipids and the Challenge of LC/MS/MS Using an Isotopically Labelled Versus Developing LC/MS Methods for Compounds with Structurally Related Internal Standard; Scott Both Hydrophilic and Hydrophobic Characteristics.; Kragerud; Autumn Dinnel; Chelsie Grochow; Ardeshir Lin Tan; Troy Voelker; Yue Zhao; Ryan S. Adler; Khadang; Chinna Pamidi; Cetero Research, Fargo, ND Cassie Ye; Min Meng; Patrick Bennett; Scott Reuschel; WP 410 Development of an Ultra Fast Online-SPE-LC-ESI- Tandem Labs, Salt Lake City, UT MS/MS Approach for Pharmacokinetic Studies; Nils WP 423 Simultaneous Determination of Plasma Helge Schebb; Bora Inceoglu; Bruce Hammock; Catecholamines Using an Integrated Strategy of an University of California, Davis, CA Automated Protein Precipitation Technique, WP 411 The Phospholipid Fix: Quantitative Measurement Reductive Ethylation Labeling and UPLC-MS/MS; and Analytical Solutions for Phosphilipid Depletion; Chengjie Ji; Max Tella; Pfizer Inc, Groton, CT Russell Grant; Patricia Holland; Brian Rappold; WP 424 Analysis of Drugs of Abuse Using Automated Labcorp, Burlington, NC Disposable Pipette Extraction and LC/MS/MS; Fred Foster 1; John Stuff1; Edward Pfannkoch1; William WP 412 A Specific 3D Conformation that Can Cause 2 2 2 Bioanalytical Challenges; Yizhong Zhang; Rick Brewer ; Sparkle Ellison ; Stephen Morgan ; Tom Gluodenis3; 1Gerstel, Inc., Linthicum, MD; 2University Steenwyk; Pfizer Inc., Groton, CT 3 WP 413 Determination of Malonyl-CoA Levels and of South Carolina, Columbia, SC; Agilent Technologies, Distribution in Rat Brain Tissue Using Liquid-Liquid Wilmington, DE 58th ASMS Conference on Mass Spectrometry Page S111 WEDNESDAY POSTERS WP 425 Determination of Nitrofuran Metabolites in Shrimp Stephen Oshea3; 1Waters Corporation, Milford, MA; by Liquid Chromatography-Tandem Mass 2Waters Corp., Manchester, UK; 3Roger Williams Spectrometry in APCI mode Using 1 Hr Sonication University, Bristol, RI Extraction; Haejung An; Mark Henry; Teresa Cain; WP 438 A Mass Spectrometry Platform to Quantitatively Darin Files; Han Chol Paek; Dennis Farley; US FDA, Profile Cancer Cell Metabolism from Cell Lines to Irvine, CA Tissues; John M Asara1, 2; Jason Locasale1; Xuemei WP 426 Method Development for Polar Embedded Polymeric Yang1; Rami Rahal2; Matthew G Vander Heiden3; 1Beth SPE; William Hudson; Paul Boguszewski; Yung-lin Israel Deaconess Medical Center, Boston, MA; Chen; Varian, Inc., Lake Forest, CA 2Harvard Medical School, Boston, MA; 3Massachusetts WP 427 A Novel Concept for Sample Collection and Sample Institue of Technology, Cambridge, MA Preparation; Stefan König; Werner Döbelin; Inovalab WP 439 Yeast Elemental, Molecular and Isotopic AG, Reinach, Switzerland Fractionation Fingerprinting: An Original Mass METABOLITE PROFILING II, 428 - 448 Spectrometry Strategy; Johann Far; Hugues WP 428 Simultaneous Determination of Nicotinic Acid and Its Preud'homme; Sylvain Berail; Ryszard Lobinski; Three Metabolites in Human Urine with Different LCABIE-UMR5254-IPREM, University of Pau, Pau, Calibration Curve Ranges by LC/MS/MS; Jiang Luo; France Jia Liu; Xiaohang Shen; Jinsong Xing; WuXi AppTec, WP 440 Analysis of Urinary Organic Acids by GC Tandem Shanghai, China Mass Spectrometry for the Accurate Diagnosis of WP 429 A Quantitative UPLC-MS/MS Method for Inborn Errors of Metabolism; Lisa Bates-Dubrow; Determining Human Metabolites of Ellagic Acid in Aaron Wheat; Patricia Whittecar; Amy Daniel; Carolyn Urine; Ken Riedl; Pratiq Patel; Jim Fuchs; Li-Shu Sparks; Leslie Wheat; Jessalyn Permenter; Stephen Wang; Gary Stoner; Steven Schwartz; The Ohio State Kahler; Frances Soderberg; Arkansas Children's University, Columbus, OH Hospital, Little Rock, AR WP 430 Approaches in Method Development and WP 441 Metabolic Profiling of Adult Urine Using Head Space- SPME-GC/MS and 1H NMR: Determination of Endogenous 25-Hydroxyvitamin D3 1 in Rat Serum by Electrospray LC-MS/MS; Rong Yi; Characterization of Gender; Shucha Zhang ; Lingyan Liu1; Debbie Steffen2; Tao Ye1; Daniel Raftery1; Hong Zhang; Gina de Boer; Xuejun Peng; Can Test Ltd, 1 2 Burnaby, Canada Purdue University, West Lafayette, IN; Center for WP 431 Lycopene Sub-Cellular Localization in Primary Authentic Science Practice in Education, West Lafayette, Prostate Epithelial and Stromal Cells; Yongchao Li1; IN Avani Vaishnav2; Linlin Dong 1; Larisa Nonn2; Richard WP 442 Quantitative Analysis of Cyanobacterial Free Fatty B. Van Breemen1; 1University of Illinois College of acids by Direct Infusion ESI; Daniel C. Brune; Shuqin Pharmacy, Chicago, IL; 2University of Illinois College Li; Xinyao Liu; James A. Garcia; Roy Curtiss III; Wim of Medicine, Chicago, IL Vermaas; Arizona State University, Tempe, AZ WP 432 Development of LC-APPI-MS/MS Methods for the WP 443 Characterization of the Populus Metabolome with an Rapid Quantitation of Retinyl Esters; Thomas LTQ-Orbitrap Mass Spectrometer; Maria Ahnlund- Hansson1, 2; Thomas Moritz1, 2; 1Swedish University of Mcdonald; Beijing Tan; Jennifer Laperle; Christopher 2 Holliman; Pfizer Inc., Groton, CT Agricultural Sciences (SLU), Umeå, Sweden; Umeå WP 433 A New Methodology for Determination of Tretinoin Plant Science Center, Umeå, Sweden in Rat Plasma Using Column Switching and LC- WP 444 Metabolic Profiling of Corn (Zea mays) Roots by Mid-Infrared Laser Ablation Electrospray Ionization MS/MS Techniques; Yansheng Liu; Moo-Young Kim; 1 Yu-Hui Fu; Chen-Yu Chung; Sarah Swenson; Darioush Mass Spectrometry; Jennifer A Day ; Bindesh Shrestha1; Peter Nemes1, 2; Akos Vertes1; 1George Dadgar; KCAS Laboratory, Shawnee, KS 2 WP 434 LC-MS/MS Method for Simultaneous Determination Washington University, Washington, DC; University of of L-Arginine (ARG), L-Citrulline (CIT), and Illinois at Urbana-Champaign, Urbana, IL Asymmetric Dimethylarginine (ADMA) in Human WP 445 Confirmation of Metabolite Identification in LC- Plasma; Yuwen Zhao1; Hsun Wen Chou1; Lina Tang1; FTMS Metabolomics Experiments Using an In-Line 1 1 2 1 Triple Quadrupole; Nicholas Carruthers1; Gilles Yuan-Shek Chen ; Kumar Ramu ; James Hui ; QPS, 1 1 LLC, Newark, DE; 2Gilead Sciences, Inc., Durham, NC Lajoie ; University of Western Ontario, London, WP 435 Simultaneous Quantitation of Histamine and 1- Canada methylhistamine Using Pre-Column Derivatization WP 446 Accurate Mass Spectrometry Based Quantification Prior to HILIC-MS/MS Analysis. Application to a and Characterization of Hepatic Microsomal Rat Brain Microdialysis Study; Emmanuel Bourgogne; Metabolism of the Thiopeptide Antibiotic GE François Xavier Mathy; David Boucaut; Hilmar 37468A; Mustafa Varoglu; Xiaowei He; Min Chu; Scott Boekens; Steven Smith; UCB pharma, Braine L'alleud, Coleman; Cubist Pharmaceuticals, Lexington, MA Belgium WP 447 Application of High Resolution Orbitrap Mass WP 436 Simultaneous Quantitation of Three Major Analyzer to Quantitation and Profiling of Amino Acrylonitrile Mercapturic Acids and Cotinine by an Acids and Short Peptides after Derivatization; Pavel Isotope-Dilution UPLC-MS/MS Method; Chia-Fang Aronov; Rabindra Tirouvanziam; Allis S. Chien; Wu; Kuen-Yuh Wu; National Taiwan University, Taipei, Stanford Univ., Stanford, CA Taiwan WP 448 Determination of 16 Anabolic Steroids Esters in WP 437 A Systematic Approach to Targeted Analysis of Equine Plasma by Liquid Chromatography- Carotenoids in the Skin of False Percula Anemone Electrospray-Tandem Mass Spectrometry; Youwen 1 2 You1; Cornelius Uboh 2; Lawrence Soma1; Fuyu Guan1; Fish Using LC/MS; Hayley Crowe ; Lucy Fernandes ; 1 1 2 1 Kate Yu1; Nancy Breen3; John Shockcor1; Henry Shion1; Xiaoqing Li ; Ying Liu ; Jeffrey Rudy ; Jinwen Chen ; Harold Pomeroy3; Bradford Bourque3; Alan Millar1; Page S112 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS 1University of Pennsylvania, West Chester, PA; 2West WP 459 Investigation of Free Radical Metabolism of Chester University, West Chester, PA Raloxifene by NADPH-fortified Human Liver DRUG METABOLISM: REACTIVE METABOLITES, Microsomes Using Liquid Chromatography/ LTQ- 449 - 465 Orbitrap; Heng-Keang Lim; Ke Yang; Silva Jose; WP 449 Identification of Novel Metabolites of Bile Acids: S- DMPK, GPCD, Johnson and Johnson PRD, Raritan, NJ Acyl Glutathione Conjugates in Rat Bile by LC-ESI- WP 460 Detection and Characterization of Covalent Protein Linear Ion Trap MS/MS; Naohiro Hori1; Kuniko Adducts by Liquid Chromatography-LTQ/Orbitrap; Mitamura1; Toshihiro Sakai1; Takashi Iida2; Shigeo Heng-Keang Lim; Wing W Lam; Jose Silva; DMPK, Ikegawa1; 1Kinki University, Higashi-Osaka, Japan; GPCD, Johnson and Johnson PRD, Raritan, NJ 2Nihon University, Tokyo, Japan WP 461 Live Single-Cell Drug (Tamoxifen) Metabolism and WP 450 Metabolism and Transport of the Glutathione Transport in an Organelle of a HepG2 Cell; Sachiko Conjugate of 4-Hydroxy-2-nonenal in Human Date; Kiyoshi Takeshima; Hajime Mizuno; Naohiro Monocytic THP-1 Cells Studied by Electrospray LC- Tsuyama; Takanori Harada; Tsutomu Masujima; MS/MS; Brandi L. Langsdorf; Cristobal L. Miranda; Jan Hiroshima Univ. BioMed., Hiroshima, Japan F. Stevens; Oregon State University, Corvallis, OR WP 462 in-vitro Metabolic Profile of Investigational WP 451 Atrazine Metabolism: Characterization of Novel Anticancer Agents by LC-Ion Trap Mass Metabolites and Glutathione Adducts Detected by Spectrometry.; Mohamed Attwa; Adnan Kadi; King LC-TOF-MS; André Leblanc; Lekha Sleno; UQAM, Saud University, College of Pharmacy, Riyadh, Saudi Montreal, Canada Arabia WP 452 Trapping Experiments with Electrochemically WP 463 Chirality Study of Homolytic Decomposition Generated Iminium Ions – Correlation to Metabolites from 15-Hydroperoxy-Eicosatetraenoic Metabolism in Human and Rat Liver Microsomes; Acid (15-HPETE) and 11-HPETE by LC-MS/MS; Kim Grimstrup Madsen1; Thomas Amos Jacobsen1; Suhong Zhang; Xiaojing Liu; Cong Wei; Clementina Steen H. Hansen2; Christian Skonberg2; Ulrik Jurva3; Mesaros; Ian A. Blair; Center for Cancer Pharmacology, Joergen Olsen4; 1NeuroSearch A/S, Ballerup, Denmark; University of Penn, Philadelphia, PA 2University of Copenhagen, Fac. Pharm. Sciences, WP 464 Use of Deuterated Napthalene and Orbitrap LC-MS Copenhagen, Denmark; 3AstraZeneca R&D Mölndal, for Identification of in vivo Biomarker Protein Mölndal, Sweden; 4Novo Nordisk A/S, Måløv, Denmark Adducts in Urine.; William T. Jewell; Nathalie Pham; WP 453 On-Line Coupling of Electrochemistry/HPLC/MS as Alan Buckpitt; UC Davis, Davis, CA an Effective Tool for the Simulation of the Oxidative WP 465 MsXelerator RM: A Software Platform for Reactive Metabolism of Alkaloids; Sandra Jahn1; Anne Metabolite Detection Using Low and High Resolution Baumann1; Torsten Vielhaber1; Bettina Seiwert2; Uwe Mass Spectrometry Data; Marco Ruijken; MsMetrix, Karst1; 1University of Münster, Münster, Germany; Maarssen, Netherlands 2University of Leipzig, Leipzig, Germany AGRICULTURE, 466 - 487 WP 454 Electrochemistry Coupled to Mass Spectrometry as a WP 466 Exploration and Comparison of Milk Whey Proteins Biomimetic Tool for the Investigation of the from Human Milk, Cow’s Milk and Infant Formula Metabolic Pathway of Pro-Hapten Skin Sensitisers; Using Non-Gel-based Proteomics Approach; Qiang Torsten Vielhaber1; Sandra Jahn1; Anne Baumann1; Zhang; Anh Le; Jeff Sanders; Mike Baim; Mead Wiebke Lohmann1; Raniero Zazzeroni2; Uwe Karst1; Johnson Nutrition, Evansville, IN 1University of Münster, Münster, Germany; 2Unilever, WP 467 Mining the Small Proteome of the Biotrophic Fungal Bedford, UK Pathogen Blumeria graminis to Reveal Effectors WP 455 Simulation of the Oxidative Metabolism of Delivered to its Host Barley; Laurence V. Triclocarban in an Electrochemical Thin Layer Cell Bindschedler1; Tim A. Burgis2; Pietro D. Spanu2; Rainer Coupled Online to LC/ESI-ToF-MS; Anne Baumann1; Cramer1; 1University of Reading, Reading, UK; Nils-Helge Schebb2; Ki Chan Ahn2; Tristan Rose2; 2Imperial college London, London, UK Bruce D. Hammock2; Uwe Karst1; 1University of WP 468 Differential Quantitation of Protein Markers in Plant Münster, Münster, Germany; 2University of California, Tissue: A Proteomic Case Study for Agriculture; Davis, CA Leah Riter1; Pamela Jensen1; Olga Vitek2; Ping Feng1; WP 456 Structural Identification of Oxidative Metabolites Susan MacIsaac1; 1Monsanto Company, St Louis, MO; and Reactive Intermediates of Trizodone by Ultra 2Purdue University, West Lafayettte, Indiana High-Resolution Q-TOF Mass Spectrometry; Qian WP 469 A Quantitative Method for Seven Polypeptide Ruan; Li Ma; Asoka Ranasinghe; Hongwei Zhang; W. Antibiotic Residues in Animal Tissues and miLk by Griffith Humphreys; Timothy Olah; Mingshe Zhu; Liquid Chromatography-Tandem Mass Bristol-Myers Squibb, Princeton, NJ Spectrometry; Joe O. Boison; Ron Gedir; Stephen Lee; WP 457 Investigating the use of Electrochemistry with Mass Johanna Matus; Canadian Food Inspection Agcy, Spectrometry To Detect Reactive Metabolites; Saskatoon, Canada Matthew Lochansky1; Annie Moran2; Randy WP 470 High-Throughput Cyclolinopeptide and Rutkowske1; Luke Miller1; 1GlaxoSmithKline, Research Triacylglycerol (TAG) Profiling of Linum Triangle Park, NC; 2University of North Carolina, usitatissimum Using LDTD-MS/MS; Julie Marr 1; Chapel Hill, NC Patrice Tremblay2; Pierre Picard3; Peta-Gaye Burnett4; WP 458 Bioactivation of 2-Acyl-5-chlorothiophene-containing Denis Paskal Okinyo Owiti4; Martin J.T. Reaney4; Compounds by Human Liver Microsomes; Weiqi 1Agilent Technologies, Mississauga, Canada; 2Phytronix Chen; Haiying zhang; Donglu Zhang; William Technologies, Quebec, QC; 3Phytronix Technologies, Humphreys; Jinping Gan; Bristol-myers Squibb, Inc., Quebec, QC; 4University of Saskatchewan, Princeton, NJ Saskatoon, Canada 58th ASMS Conference on Mass Spectrometry Page S113 WEDNESDAY POSTERS WP 471 Dicamba O-demethylase Monooxygenase (DMO) ARS-DB NRRC, Stuttgart, AR; 3USDA-ARS-SRRC, New Substrate Specificity Studies Using UPLC-Mass Orleans, LA Spectrometry; Yanfei Wang; Mason Hughes; Ryan WP 482 Structural Investigation of Synthetic Lignins by Bartlett; Monsanto Company, Chesterfield, MO Nano-Electrospray Ionization Quadrupole Time-of- WP 472 Determination and Risk Assessment of Arsenic Flight Tandem Mass Spectrometry; Chamnongsak Compounds in Agricultural Products by Anion- Chanthamontri1; Timothy Zwier1; John Ralph2; Scott A. Exchange Liquid Chromatography with Inductively Mcluckey1; 1Purdue University, West Lafayette, IN; Coupled Plasma-Mass Spectrometry; Sung-Kug Park; 2University of Wisconsin-Madison, Madison, Wisconsin Hoon Choi; Hee-Ra Park; Ock-Jin Paek; Young-Woon WP 483 High Throughput Analysis of Melamine in Infant Kang; Cheon-Ho Jo; Dong-Sul Kim; Korea Food and Milk Powder Formula Using LDTD-MS/MS; Serge Drug Administration, Seoul, South Korea Auger; Pierre Picard; Patrice Tremblay; Gregory WP 473 Applications of UHPLC Orbitrap-MS for the Blachon; Sylvain Letarte; Phytronix Technologies inc., Multiresidue Screening of Pesticides in Agricultural Québec, Canada Commodities; James Chang1; Jon Wong2; 1Thermo WP 484 Effect of Internal Standard on the Accuracy of Fisher Scientific, San Jose, CA; 2FDA-CFSAN, College Vitamin D Measurement by Liquid Chromatography Park, MD Tandem Mass Spectrometry (LC/MS/MS); Min WP 474 Screening for Pesticides in Food and Water Samples Huang; Doug Winters; Covance, Nutrition Chemistry with Identification based on Library Searching Using and Food Safety, Madison, WI Accurate Mass LC/MS/MS; Andre Schreiber1; Yun WP 485 LC-MS/MS Based Targeted Profiling of Plant Yun Zou1; Doina Caraiman1; Tanya Gamble1; Rebecca Hormones in Stressed Arabidopsis and Tomato Wittrig2; Paul Yang3; 1AB SCIEX, Concord, Canada; Leaves; Chao-Jung Chen1; Yit-Wai Mak2; Yet-Ran 2Restek, Bellefonte, PA; 3Minstry of Environment, Chen1; Sheng-Yu Huang3; Kefei Wang4; 1Agricultural Toronto, Ontario, Canada Biotech Research Ct, Academia Sinica, Taipei, Taiwan; WP 475 Comparative Assessment of ToF Profiling and 2Scientific Instrument Ct, Academia Sinica, Taipei, Tandem Quadrupole Mass Spectrometric Screening Taiwan; 3Mass Solutions Technology, Taipei, Taiwan; of Pesticides in a Vegetable Matrix.; Stephen Wong1; 4Thermo Fisher Scientific, San Jose, CA Evelyn Goh1; Mark Ritchie1; Eleanor Riches2; Peter WP 486 Determination of the Secondary Metabolite Hancock2; James Morphet2; 1Waters Pacific Pte Ltd, Bikaverin from Maize by LC-MS/MS; Mark Busman; Singapore, Singapore; 2Waters Corporation, Northwich, USDA-ARS, Peoria, IL UK WP 487 Multi-Residue Analysis of Veterinary Drugs in WP 476 Multi Residue Pesticide Screening and Confirmation Animal Produce by Hybrid Triple Quadrupole in Food Using LC/MS/MS System and Library Linear Ion Trap LC-MS/MS; Pavel Metalnikov; Irina Searching; Hyunjeong Cho1; Gijun Park1; Byungchul Bogdanova; Alexander Komarov; Alexander Panin; Son1; Youngbae Son1; Sunghoon Yeo2; Hansoon Kwon2; VGNKI, Moscow, Russian Federation 1 National Agricultural Products Quality Management, FLAVORS AND FRAGRANCES, 488 - 492 2 Seoul, South Korea; AB SCIEX Korea, Seoul, South WP 488 A GC/MS Tailored Multivariate Approach to the Korea Analysis of Trace Odor Compounds in Green Tea; WP 477 Study of Qualitative Screening Analysis of Takeshi Serino1; Sadao Nakamura1; Anthony Gray2; Organophosphorus Pesticides Residues in Foods David Peterson2; 1Agilent Technologies, Tokyo, Japan; Using a GC-TOFMS with Fast GC Technique; 2Agilent Technologies, Santa Clara, CA Masaaki Ubukata1; Yoshiyuki Itoh1; Jun Tamura1; 1 1 3 WP 489 Volatile Composition and Organoleptic Qualitative Yoshihisa Ueda ; Jun Onodera ; Bob DiPasquale ; Evaluation of Rose Species by Live Single Cell Direct Robert Hertsens2; 1JEOL Ltd., Akishima, Japan; 2JEOL 3 Mass Spectrometry; Mónica Lorenzo Tejedor; Hajime (Europe) B. V., Zaventem, Belgium; JEOL USA Inc., Mizuno; Naohiro Tsuyama; Takanori Harada; Tsutomu Boston, MA Masujima; Hiroshima Univ. BioMed., Hiroshima, Japan WP 478 Electrospray MS as an Effective, Quantitative WP 490 Identification of Non-Volatile Congeners by Accurate Method for Tracking Root Exudates Throughout a Mass LC-TOF to Determine Bourbon Product Plant's Growth Cycle; Sharon Curtis1; Paul Mayer1; 1 2 2 2 2 Authenticity; Luke Adam ; Sue Dantonio ; Lynne Savka Orozovic ; Morris Switzer ; Carlos Monreal ; Marshall2; Andre Szczesniewski2; 1Beam Global Spirits, Justin Renaud1; 1University Of Ottawa, Ottawa, Canada; 2 2 Louisville, KY; Agilent Technologies, Schuamburg, IL Agriculture and Agri-Food Canada, Ottawa, Canada WP 491 Quantitative Analysis and Confirmation of Presence WP 479 Distribution of Almond Phenolics in Skins and Water of Some Migrants in Food Packaging; Philippe during Blanching as Quantified by LC-TOF MS; 1 1 2 2 1 1 1 Tourelle ; Gilles Jarry ; Cécile Busset ; Stephen Lock ; Christine A. Hughey ; Carina Minardi ; Lorenzo Reyes ; 1Impress Metal Packaging, La Flèche, France; 2AB Anuradha Prakash1; Bruce Wilcox2; 1Chapman 2 SCIEX, Paris, France University, Orange, CA; Applied Proteomics, Inc., WP 492 Distinguishing Among Three Luteolin Glycoside Orange, CA Isomers by Using Host-Guest Chemistry and LC- WP 480 Analysis of Organic Volatile Compounds in Brazilian 1 2 MS/MS; Canan Ozer; Dil Ramanathan; Kean University, Stingless Bee Honey; Ildenize Cunha ; Jose L. P. Jará ; Union, NJ Marcos N Eberlin2; 1ThoMSon Mass Spectrometry HOMELAND SECURITY, 493 - 514 Laboratory - UNICAMP, Campinas, Brazil; 2ThoMSon Lab UNICAMP, Campinas, Sp, Brazil WP 493 Development of Ion Mobility Separation and Mass WP 481 Single Kernel Method for Detection of 2-acetyl-1- Spectrometric Conditions in a Q-Tof Instrument for the Analysis of Chemical Warfare Agents; Paul pyrroline in Aromatic Rice Germplasm Using SPME- GC/MS; Rolfe Bryant1, 2; Anna Mcclung1, 2; Casey C. D'agostino; Claude Chenier; DRDC Suffield, Medicine Grimm3; 1USDA-ARS-DB NRRC, Stuttgart, AR; 2USDA- Hat, Canada Page S114 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS WP 494 Improving Selectivity and Sensitivity of Anti- WP 507 GC-MS Analysis of Carbohydate and Fatty Acid Terrorism Detection Equipment with FAPA-IMS- Marker Abundance in Ricin Toxin Preparations; IMS; Jonathan Dilger1, 2; Jeff Everett1; Jake Shelley1; Kate C. Antolick; David S. Wunschel; Heather A. Stephen Valentine1; Gary M. Hieftje1; David E. Colburn; Helen W. Kreuzer; James J. Moran; Angie M. Clemmer1; 1Indiana University, Bloomington, IN; 2Naval Melville; Pacific Northwest National Laboratory, Surface Warfare Center, Crane Division, Crane, IN Richland, WA WP 495 Ambient Direct Analysis in Real Time with a WP 508 Gas Chromatographic-Negative Ion Chemical Monolithic Ion Mobility Spectrometry (DART-IMS) Ionization Mass Spectrometric Analysis of Sulfur Platform for Chemical Agent Monitoring; Glenn A Mustard-Plasma Protein Adducts in Rabbits from Harris; Facundo Fernandez; Georgia Institute of Ocular Vapor Exposure; Richard Lawrence; Bethany Technology, Atlanta, GA Williams; Denise Milhorn; Benedict Capacio; US Army WP 496 Quantitation of Alkyl Methylphosphonic Acids as MRICD, Aberdeen Proving Ground, MD Free Metabolites of Organophosphorus Nerve Agents WP 509 Comparison and Evaluation of Sample Preparation in Rat and Human Urine by LC/ESI/MS/MS; Sophie Methods for Identification of Highly Pathogenic Gougeon; Cécile Montauban; Didier Christin; Laurent Bacteria Using MALDI–ToF Mass Spectrometry; Taysse; Anne Bossee; DGA NRBC Defence, Vert-Le- Michal Drevinek1; Jiri Dresler2; Hana Placakova1; Petit, France Martin Hubalek3; 1Natl Inst for NBC Protection, Milin, WP 497 Multimode Ion Source for Low Level Detection of Czech Republic; 2Central Military Institute of Health, Threat Agents; Vadym Berkout; Thomas Saul; Prague, Czech Republic; 3Institute of Molecular Vladimir M. Doroshenko; SESI, Inc., Columbia, MD Pathology, Univ. of Defense, Hradec Kralove, Czech WP 498 Advances in Mobile GC/MS Technology for Republic Homeland Security and Defense; Garth Patterson; WP 510 Quantification of Botulinum Neurotoxins A and B: Cynthia Liu; Mark Gregory; Mitch Wells; Dennis From Method Development to Applications in Barket; ICx Griffin, West Lafayette, IN Clinical Samples; Bryan Parks; Jakub Baudys; Wanda WP 499 Use of RAMFAC (Rapid Multivariate Factorization) Santana; Suzanne Kalb; John R. Barr; Centers for Deconvolution Algorithm In a Portable GC/MS Disease Control and Prevention, Atlanta, GA Instrument for Compound Identification; Joe WP 511 High-Salt Washing Significantly Improves the Oliphant1; Edgar Lee1; Dennis Tolley2; Cory Grant1; Detection of BoNT/A in Stool Matrix by Mass Chad Grant1; 1Torion Technologies, American Fork, UT; Spectrometry; Dongxia Wang; Jakub Baudys; Suzanne 2Brigham Young University, Provo, Utah Kalb; John R. Barr; Centers for Disease Control and WP 500 A LVI– GC– EI/MS - Selective Detectors Apparatus Prevention, Atlanta, GA for Enhanced Sensitivity in Detection and WP 512 Application of a Label-Free Mass Spectrometry Identification of Chem.-Terror Agents; Nitzan Method to Measure Relative Quantities of Proteins Tzanani; Dana Marder; Hagit Prihed; Israel Inst. for within Botulinum Neurotoxin Complexes; Hercules Biological Research, Ness Ziona, Israel Moura; Adrian R Woolfitt; Rebecca R Terilli; Maria WP 501 Effect of Prednisolone for Mitigation of Solano; John R. Barr; Centers for Disease Control & Neovascularization of the Cornea Following Prevention, Atlanta, GA Exposure to HD by use of Quantitative Shotgun WP 513 The Use of Mass Spectrometry to Determine the Proteomics; Mitchell Meade1; Pavel Shiyanov1; Michael Enzymatic Target of Clostridium baratii Type F Babin2; 1AFRL, Dayton, OH; 2Battelle, Columbus, OH Neurotoxin; Suzanne R. Kalb1; Jakub Baudys2; WP 502 Comparison of LC-MS/MS Strategies for Nerve Christina Egan3; Theresa J. Smith4; Leonard A. Smith4; Agent Metabolites Analysis; John Tokarz; Kevin J. John R. Barr1; 1CDC, Atlanta, GA; 2Battelle Memorial Shefcheck; Joy M. Ginter; US Army - ECBC, Aberdeen Institute, Atlanta, GA; 3Wadsworth Center, New York Proving Ground, MD State Dept. of Health, Albany, NY; 4Integrated WP 503 Detection of Ribosomal Inactivating Proteins via Toxicology, USAMRIID, Ft. Detrick, MD Immuno-precipitation and MALDI-TOF MS; James WP 514 Carbohydrate Analysis by MALDI-MS and GC-MS Robertson; Roman Aranda IV; Shauna M Dineen; Jason to Detect Potential Markers of Organism Processing Bannan; FBI Laboratory, Quantico, VA and Preservation; Angela M. Melville; David S. WP 504 Forensic Characterization and Source Wunschel; Heather A. Colburn; Christina M. Sorensen; Apportionment of Endogenous Peptides Derived Casey L. Stamper; Nancy B. Valentine; Heather E. from Biological Threat Agents; Brian H. Clowers; Engelmann; Kate C. Antolick; Karen L. Wahl; Pacific Cinnamon D. Bolz; Heather Engelmann; David S. Northwest National Laboratory, Richland, WA Wunschel; Angela M Melville; Nancy B. Valentine; POLYMER ANALYSIS, 515 - 527 Christina Sorensen; Karen L. Wahl; Pacific Northwest WP 515 Complementary Mass Spectrometric Techniques for National Laboratory, Richland, WA the Characterization of Intractable Polymers; Patricia WP 505 Simultaneous Profiling and Quantitation of 1 M. Peacock; Dupont, Wilmington, DE Phosphonic Acids; Keith Goodman ; Anthony WP 516 Characterization of Butyl Rubber Thermal- Romanelli1; Patrick South2; Mark Bauer2; 1AB SCIEX, 2 Oxidative Decomposition Products; James Hochrein; Framingham, MA; Battelle, Columbus, OH Robert Bernstein; Michael White; Shawn Dirk; Steven WP 506 Investigation Using SIMION on the Implementation Thornberg; Cody Washburn; Sandia National of a Cubic Ion Trap as an Ionization Chamber on a 1, 2 3 Laboratories, Albuquerque, NM GC/LIT; Sarah Vitcher ; Claude Beaugrand ; Laurens WP 517 A Study of Structure and Thermal Degradation of Dudragne2; Roberta Collino2; Jean-Claude Tabet1; 1 1 2 Flame Retardant Polycarbonate; Yoshiyuki Itoh ; University Paris VI (UPMC), Paris, France; Thales Hiroaki Sato2; Masaaki Ubukata1; Kanae Teramoto1; Jun Security Solutions & SystemS, Vélizy-Villacoublay, 1 1 2 3 Tamura ; JEOL Ltd., Akishima, Japan; AIST, Tsukuba, France; ALPHA-MOS, Toulouse, France Japan 58th ASMS Conference on Mass Spectrometry Page S115 WEDNESDAY POSTERS WP 518 Dual Measurement of Polymer and Peptide Thermal Cutting GC/MS; Andrew Tipler; Adam J. Patkin; Decomposition by Calorimetry and Laser Desorption William Goodman; PerkinElmer, Shelton, CT Ionization Using a Single Microfabricated Device WP 531 Removing the Need for Extractions in the Analysis of Platform; Curtis Mowry; Matthew Moorman; John Pesticides Using Triple Quadrupole GC/MS System; Anderson; Jeffrey Reich; Sandia National Laboratories, Eric Phillips; ThermoFisher Scientific, Austin, TX Albuquerque, NM WP 532 Mars Organic Molecule Analyzer: Detecting WP 519 Time of Flight Secondary Ion Mass Spectrometric Organics in Extra-Terrestrial Soil via Low Power Determination of Molecular Weight Distribution of Gas Chromatography-Mass Spectrometry; Veronica Low Polydispersity Poly(Dimethylsiloxane) with Pinnick 1; Arnaud Buch2; Friso H. W. Van Amerom3; Polyatomic Primary Ions; Hye Kyoung Moon; David Ryan M. Danell4; Luann Becker5; Robert J. Cotter1; D. Wells; Joseph A. Gardella, Jr.; State University of 1Middle Atlantic MS Laboratory, Baltimore, MD; 2Ecole New York at Buffalo, Buffalo, NY Centrale Paris, Paris, France; 3SRI International, St. WP 520 Fragmentation of Polyacrylates by Tandem ESI- Petersburg, Florida; 4Danell Consulting, Greenville, FTMS; Sasa Miladinovic1; Cynthia Kaeser2; Charles L. NC; 5John's Hopkins University, Baltimore, MD Wilkins1; 1University of Arkansas, Fayetteville, AR; WP 533 Withdrawn 2University of the Cumberlands, Williamsburg, KY WP 534 Mass Spectra of the Derivatives of Biological Buffer WP 521 Mechanistic Study of the Collision-Induced Components by GC-MS; Yufang Zheng; Edward Dissociation of Sodium-Cationized Polylactide White V; Stephen E. Stein; NIST, Gaithersburg, MD Oligomers : A Joint Experimental and Theoretical WP 535 Methods for the Detection of Benzenedithiols and Investigation; Julien De Winter; Vincent Lemaur; Their Analogs by GC-MS; Kirill Tretyakov1; Roman Philippe Marsal; Olivier Coulembier; Jérôme Cornil; Borisov2; Nino Todua1; Vladimir Zaikin2; Stephen Philippe Dubois; Pascal Gerbaux; University of Mons, Stein1; Anzor Mikaia1; 1National Institute of Standards Mons, Belgium and Technology, Gaithersburg, MD; 2Topchiev Institute WP 522 CAD Versus ETD Fragmentation Pathways of of Petrochemical Synthesis RAS, Moscow, Russia Polyesters; Vincenzo Scionti; Chrys Wesdemiotis; The WP 536 Determination of Indole in Seafood by Headspace University of Akron, Akron, OH SPME GC-MS; F. Aladar Bencsath; Ann Abraham; WP 523 Characterization of Copolymers by Mass and Ronald A. Benner, Jr.; FDA, Gulf Coast Seafood Lab, Tandem Mass Spectrometry; Aleer M. Yol; Roderic P. Dauphin Island, AL Quirk; Chrys Wesdemiotis; Jon E. Janoski; The WP 537 Electron Ionization Mass Spectra of Derivatives of University of Akron, Akron, OH Sulfabenzamide and Related Compounds: Unusual WP 524 Influence of the End-Group on the MS/MS Rearrangement Involving Migration of Carbonyl O- Dissociation of Electrosprayed Atom; Nino Todua1; Kirill Tretyakov1; Roman Borisov2; Poly(Dimethylsiloxane) Oligomers: Hydride vs Dmitry Zhilyaev2; Vladimir Zaikin2; Stephen Stein1; Trimethylsilyl End-Groups; Thierry Fouquet; Stéphane Anzor Mikaia1; 1National Institute of Standards and Humbel; Laurence Charles; University Aix-Marseille I & Technology, Gaithersburg, Maryland; 2Topchiev III, Marseille, France Institute of Petrochemical Synthesis RAS, Moscow, WP 525 Mass Spectrometric Investigations of Bisphenol A Russia and Other Migrants in Canned Food; Luke K. WP 538 Use of Deuterated O-n-butyl-hydroxylamines in the Ackerman; Gregory Noonan; John Roach; Eugene Analysis of Fructosylamino Acids Provide GLC/MS Mazzola; Pierluigi Delmonte; Timothy Begley; FDA Characteristics that Improve Separation, Center for Food Safety, College Park, MD Identification and Verification; Thomas P. WP 526 Selectivities Estimation of tritonX-114-Co(II) Mawhinney; Deborah Chance; Valeri Mossine; Nancy Complex by Electrospray Ionization Fourier Cassity; James Waters; University of Missouri, Transform Ion Cyclotron Resonance (ESI-FTICR) Columbia, MO Mass Spectrometry; Khaled Edbey 1; Grainne Moran2; WP 539 Low-Pressure Positive Chemical Ionization GC/MS Gary D. Willett2; 1University of Garyounis, Benghazi, for the Analysis of Lipids from Chromulina Libya; 2University of New South Wales, Sydney, freibugensis Defl., A Potential Biofuel Source.; Australia Douglas Cameron; Ashley Stewart; Montana Tech, WP 527 Qualitative Analysis of the Additives in Butte, MT Polypropylene Film by MALDI-MS Combined with WP 540 Development of a Sensitive and Reliable Method for Micro HPLC Fractionation; Hirotaka Shioji; the Measurement of Drugs of Abuse comparing GC- Yoshihiko Taguchi; Nobuyuki Sato; Toray Research TOF/MS vs. GC- Quadrupole/MS; Petra Gerhards1; Center, Inc., Otsu, Japan Juergen Sawazki3; Gerhard Horner2; Pierre Schanen2; 1 2 GCMS DEVELOPMENTS AND APPLICATIONS, 528 - 548 ALMSCO International, Llantrisant, UK; five 3 WP 528 5975-SMB - A New Type of GC-MS with Advanced technologies, Munich, Germany; Apotheke der LVR Capabilities; Aviv Amirav1; Alexander B. Fialkov2; Klinik Viersen, Viersen, Germany Alexander Gordin2; Tal Alon2; 1Tel-Aviv University, Tel- WP 541 Headspace-GC/MS Quantification of Furan in Rat Aviv, Israel; 2Tel Aviv University, Tel Aviv, Israel Blood and Liver; Mona I. Churchwell; Linda S. Von WP 529 Quantitative Solid Phase Microextraction Analysis of Tungeln; Daniel R. Doerge; Nat. Ctr. Tox. Res., Volatiles and Semivolatiles Using field Portable Gas Jefferson, AR Chromatography-Toroidal Ion Trap Mass WP 542 Analysis of Polybrominated Biphenyls in Spectrometry (SPME-GC-TMS); Christopher R. Pharmaceutical Manufacturing Process Samples Bowerbank; Joseph L. Oliphant; Edgar D. Lee; Douglas Using GC-MS; Chen Ding; Adrian Anderson; Gang W. Later; Torion Technologies Inc., American Fork, UT Zhao; Russel Cink; Qunying Zhang; Nancy Benz; Abbott WP 530 Reduced Sample Preparation for Determination of Laboratories, North Chicago, IL N-Nitrosamines in Food and Beverages with Heart- Page S116 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS WP 543 Trace Level Determination of Phenyl Chloroformate Nicoletta Schintu1; Anna Nilsson2; Per Svenningsson1; as a Potential Genotoxic Impurity in Process Related Per E. Andren2; 1Karolinska Institute, Stockholm, Intermediates and Drug Substance; Qizhi (Kelly) Hu; Sweden; 2Uppsala University, Uppsala, Sweden Susanna Lai; Sheng Cui; David Yeung; Xiao-Keng Liu; WP 556 Imaging MS in Studies of Pancreatic Cancer; Patrícia Fang Wang; Tiffany Correll; Amgen, Inc., Thousand V. Abdelnur1, 2; Michelle L. Reyzer1; Lisa M. Manier 1; Oaks, CA Erika E. Frazier1; Nipun Merchant1; Marcos N. Eberlin2; WP 544 Electron Ionization Mass Spectra and Retention Richard M. Caprioli1; 1Vanderbilt Univ Sch of Med, Indices of Phthalate Monoesters and Derivatives; Nashville, TN; 2ThoMSon Lab UNICAMP, Campinas, Yuxue Liang; Yufang Zheng; Edward White V; Stephen SP, Brazil Stein; National Institute of Standards and Technology, WP 557 Mass Spectrometric Imaging of Gaithersburg, MD Lysophosphatyidylcholines in Various Tissue Types; WP 545 Investigation of Hazardous, Volatile Hydrocarbons in Robert F. Menger1; Whitney L. Stutts1; John A. Commercial Beverages; Sarah J Saylor1; Catherine Bowden2; Dhanam Anbukumar2; David A. Ford2; Bentzley1; Alexander V. Kachur1, 2; 1University of the William W. Dawson3; Timothy J. Garrett4; Richard A. Sciences in Philadelphia, Philadelphia, PA; 2University Yost1; 1University of Florida, Department of Chemistry, of Pennsylvania, Philadelphia, PA Gainesville, FL; 2St Louis University School of WP 546 High Sensitivity Multi-Residue Pesticide Analysis in Medicine, St Louis, MO; 3University of Florida, Fruit Preserve Using GC-MS/MS; Hans J. Department of Ophthamology, Gainesville, FL; Huebschmann; Thermo Fisher Scientific, Austin, TX 4University of Florida, College of Medicine, Gainesville, WP 547 The Application of APGC TOF MS in the Analysis of FL Archeological Biomarkers Associated with Bitumens WP 558 High Field FTMS as a Tool in the Identification of in Egyptian Mummies; Steve Smith1; Hilary J. Major1; Neuroncological Lipid Biomarkers; Katherine A. Keith Hall2; Natalie McCreesh3; Andrew Gize3; Tony Kellersberger1; Nathalie Y.R. Agar2; 1Bruker Daltonics, Newton1; 1Waters Corporation, Manchester, UK; 2Hall Billerica, MA; 2Brigham and Womens Hospital Harvard Analytical, Manchester, UK; 3The University of Medical School, Boston, MA Manchester, Manchester, UK WP 559 High Mass Resolution Imaging and Metabolic IMAGING MS: BIOMOLECULES, 548 - 583 Profiling of Flowers of Arabidopsis Cuticle Mutant; Zhihong Song 3; Natasha D. Brohier2; Ji Hyun Jun2; WP 548 Direct Molecular Analysis of Biomass Samples Using 1 2 2 MALDI Imaging Mass Spectrometry; Yanfeng Chen; Edward S. Yeung ; Young Jin Lee ; Basil J. Nikolau ; 1Ames Lab of US DOE, Ames, IA; 2Iowa State Jung Seokwon; Mark Cameron Sullards; Arthur 3 Ragauskas; Georgia Institute of Technology, Atlanta, GA University, Ames, IA; Ames Lab of US DOE/Iowa State WP 549 Analysis of in situ Trypsin-Digested Amyloid Beta University, Ames, IA Peptides by MALDI-LIT-MSn for Mass Spectral WP 560 Molecular Pathology Using Desorption Electrospray Imaging; Daniel P. Magparangalan1; Timothy J. Ionization Imaging Mass Spectrometry From the 1 2 1 Lipid Profiles of Human Bladder and Kidney Garrett ; Dieter M. Drexler ; Richard A. Yost ; 1 1 1University of Florida, Gainesville, FL; 2Bristol-Myers Cancers; Allison Dill ; Livia S Eberlin ; Anthony Costa1; Cheng Zheng1; Demian R. Ifa1; Liang Cheng2; Squibb, Wallingford, CT 2 2 1 WP 550 Comparative Study of the Molecular Content of Islets Timothy Masterson ; Michael Koch ; Olga Vitek ; R. Graham Cooks1; 1Purdue University, West Lafayette, IN; of Langerhans between ob/ob and Normal Mouse 2 Pancreas with MALDI-IMS and LC/MS.; Laurens Indiana University School of Medicine, Indianapolis, IN Minerva; Kurt Boonen; Geert Baggerman; Lut Arckens; WP 561 Differentiation of Soft Tissue Tumors Using Imaging K.U.Leuven, Leuven, Belgium Mass Spectrometry; Stefan Willems; Alexandra van WP 551 High-Mass Accuracy MALDI Imaging of Trypic Remoortere; René van Zeijl; André Deelder; Liam Peptides - Identification by On-Tissue MS/MS and Mcdonnell; Pancras Hogendoorn; LUMC, Leiden, LC/MS Measurements; Yvonne Schober1; Kerstin Netherlands Strupat2; Spengler Bernhard1; Andreas Römpp1; 1Justus WP 562 Mapping Lipid Alterations in Traumatically Injured Liebig University, Giessen, Giessen, Germany; 2Thermo Rat Spinal Cord by desorption Electrospray Fisher Scientific, Bremen, Germany Ionization Imaging Mass Spectrometry; Marion WP 552 MALDI Mass Spectrometry Imaging of Penile Girod; Yunzhou Shi; Ji-Xin Cheng; R. Graham Cooks; Cancer Tissue; Brian Flatley1; Peter Malone2; Rainer Purdue University, West Lafayette, IN Cramer1; 1University of Reading, Reading, UK; 2Royal WP 563 Abnormal Alteration of Brain Lipids in the Scrapper- Berkshire NHS Foundation Trust Hospital, Reading, UK Gene Knockout Mice Revealed by Imaging Mass Spectrometry Coupled with Principle Component WP 553 MALDI Imaging of Post Mortem Human Spinal 1 2 3 Cord in Amyotrophic Lateral Sclerosis; Jörg Analysis; Ikuko Yao ; Yuki Sugiura ; Mitsutoshi Setou ; 1Kansai Medical University, Osaka, Japan; 2Tokyo Tech, Hanrieder; Analytical Chemistry, Uppsala University, 3 Uppsala, Sweden Yokohama, Japan; Hamamatsu School of Medicine, WP 554 The Distribution of the alphaA-crystallin Protein and Hamamatsu, Japan Its Truncation Products in Tissue Sections from a WP 564 Mass Spectrometric Imaging of Glycosphingolipids 1 from Breast Cancer Tissue Sections in Ion Mobility rodent Model of Cataracts; David R. Stella ; Kyle A. 1 Floyd1; Angus C. Grey2; Matthew B. Renfrow1; Kevin Separation Mode; Kamila Czornak ; Tiffany R. 3 1 1 Blackwell2; Lu Jiang2; Kristine Glunde2; Ron M.A. L. Schey ; Stephen Barnes ; University of Alabama at 1 1 2 Birmingham, Birmingham, Alabama; 2University of Heeren ; AMOLF, Amsterdam, Netherlands; Johns Auckland, Auckland, New Zealand; 3Vanderbilt Hopkins University School of Medicine, Baltimore, MD University, Nashville, TN WP 565 MALDI Imaging of Sub-20 Micron Lipid WP 555 Brain Alterations of Neuropeptides in an Distribution in Tissue Using Lasers Operating Experimental Mouse Model of Parkinson’s Disease; Between 1kHz and 10kHz; Jeff Brown; Paul Murray; 58th ASMS Conference on Mass Spectrometry Page S117 WEDNESDAY POSTERS Emmanuelle Claude; Daniel Kenny; Waters Micromass Tissue Proteome Using Alzheimer’s Disease Model; MS Technologies, Manchester, UK Fiona Plows1; Mariana Rusa 1; Matt Hammond1; Steve WP 566 Investigation of Tuberculosis Infection and Response Roth1; Amanda Bulman1; Enrique Dalmasso1; Martin to Therapy by Imaging Mass Spectrometry; Michelle Schuerenberg2; Detlev Suckau2; 1Bio-Rad Laboratories, L. Reyzer1; Jamie L. Allen1; Laura Via3; JoAnne L. Inc., Hercules, CA; 2Bruker Daltonics, Bremen, Flynn2; Clifton E. Barry, III3; Richard M. Caprioli1; Germany 1Vanderbilt University, Nashville, TN; 2University of WP 576 Localization and Identification of Novel Peptides in Pittsburgh, Pittsburgh, PA; 3NIAID, NIH, Bethesda, MD Hirudo Medicinalis Using MALDI Imaging.; Jocelyne WP 567 Lipid Topography of Colonic Mucosa by TOF-SIMS Bruand1; Srinivas Sistla1; Pieter Dorrestein 2; Majid Imaging in Cftr Knockout Mice; Mario Ollero1, 2; Ghassemian1; Maxence Wisztorski3; Michel Salzet4; Alexandre Seyer1; Marc Brulet1; Alain Brunelle1; Janine Terry Gaasterland1; Eduardo Macagno1; Vineet Bafna5; Fritsch2, 3; Aleksander Edelman2, 3; Olivier Laprévote1, 3; 1UCSD, La Jolla, CA; 2University of California, San 1Centre de Recherche Gif, ICSN-CNRS, Gif-sur-Yvette, Diego, Skaggs School, La Jolla, CA; 3University of Lille France; 2INSERM, U845, Paris, France; 3Université 1, Villeneuve D'ascq, France; 4University, Villeneuve Paris Descartes, Paris, France D'ascq Cedex, France; 5University of California San WP 568 Enhanced Visualization of Phospholipids in Animal Diego, San Diego, CA and Plant Tissue Sections by Using Ionic Liquid WP 577 Development of Methods for Quantitative MALDI Matrixes in MALDI Mass Spectrometry; Kamlesh Imaging by MRM for Absolute Spatial Quantitation Shrivas; Takahiro Hayasaka; Mitsutoshi Setou; of Proteins: Application to Breast Cancer Tissues; Hamamatsu University School of Medicine, Hamamatsu, Elizabeth Clemis1; Derek Smith1; Ryan M. Danell2; Japan Christoph Borchers1; 1UVic Genome BC Proteomics WP 569 Visualizing Sphingolipids in Porcine Ocular Lens by Centre, Victoria, Canada; 2Danell Consulting, MALDI High-Resolution Mass Spectrometry Greenville, North Carolina Imaging; Jaroslav Pol1, 2; Veronika Vidova1; Michael WP 578 3D Spatial Distribution of Synthetic Volny 1; Petr Novak1; Vladimir Havlicek1; Susanne K Oligodeoxynucleotide in Tumor-Bearing Mice: An Wiedmer2; Juha Holopainen2; 1Institute of Microbiology, Imaging Mass Spectrometry Study; Rita Casadonte 1; Prague, Czech Republic; 2University of Helsinki, Joseph M. Amman 1; Jennifer Grandis2; David Carbone Helsinki, Finland 1; Richard M. Caprioli3; 1Vanderbilt University, WP 570 Identification and Visualization of Phospholipids in Nashville, TN; 2University of Pittsburgh School of Tissue Sections Using MALDI, NALDI and DESI Medicine, Pittsburgh, Pennsylvania; 3Vanderbilt Univ Mass Spectrometry Imaging: A Comparative Study; Sch of Med, Nashville, TN Veronika Vidova1, 2; Michael Volny 1; Petr Novak1, 3; WP 579 Application of MALDI Mass Spectrometry Tissue Martin Strohalm1; Jaroslav Pol1, 4; Vladimir Havlicek1, 2; Imaging to Discovery Biomarkers for Diagnosis and 1Institute of Microbiology, Prague, Czech Republic; Prognosis of Alzheimer’s Disease; Sun Yong Jeong 1, 2; 2Palacky University, Olomouc, Czech Republic; Eric Hamlett1; Oscar Alzate1, 2; 1Department of Cell and 3Charles University, Prague, Czech Republic; Development Biology, UNC, Chapel Hill, NC; 2UNC- 4University of Helsinki, Helsinki, Finland Systems Proteomics Center, Chapel Hill, NC WP 571 Imaging of Abundant Human Lens Lipids by WP 580 Uncovering the Proteome of Late Stage Prostate Desorption Electrospray Ionization Mass Cancer by Imaging Mass Spectrometry; Kristina Spectrometry; Shane Ellis1; Chunping Wu2; Jane M. Schwamborn1; Peter Wild2; Richard M. Caprioli1; Deeley1; Xiangjia Zhu3; Roger Truscott3; Todd W 1Vanderbilt University, Nashville, TN; 2Institute of Mitchell1; R. Graham Cooks 2; Stephen J Blanksby1; Surgical Pathology, Zurich, Switzerland 1University of Wollongong, Wollongong, Australia; WP 581 Investigation of the Cutaneous Proteome in Humans: 2Purdue University, West Lafayette, IN; 3Save Sight Imaging MS of Biopsies from Skin Ulcers; Domenico Institute, University of Sydney, Sydney, Australia Taverna1; Alonda C. Pollins2; Lillian B. Nanney2; WP 572 Selective MALDI Imaging Mass Spectrometry of Giovanni Sindona1; Richard M. Caprioli2; 1University of Lipids by Adding Lithium Salts to the Matrix Calabria, Arcavacata Di Rende, Italy; 2Vanderbilt Solution; Vincent Guérineau1; David Touboul1; Alain University, Nashville, TN Brunelle1; Olivier Laprévote1, 2; 1Centre de Recherche de WP 582 Sub-Micrometer ToF-SIMS Mass Spectrometry Gif, ICSN-CNRS, Gif-sur-Yvette, France; 2Université Imaging of Lipid Droplets during Intestinal Paris Descartes, Paris, France Absorption; Alexandre Seyer1; David Touboul1; WP 573 Optimization of the Collection Efficiency of Christine Coméra2; Xavier Collet2; Alain Brunelle1; Secondary Ions for Spatially Resolved Secondary Ion Olivier Laprévote1, 3; 1Centre de Recherche de Gif, Mass Spectrometry in Crossbeam Devices; Dirk ICSN-CNRS, Gif-Sur-Yvette, France; 2INSERM U563, Preikszas1; Michel Aliman1; Hubert Mantz1; Albrecht Toulouse, France; 3Université Paris-Descartes, Paris, Brockhaus2; Alexander Laue2; 1Carl Zeiss NTS GmbH, France Oberkochen, Germany; 2University of Wuppertal, WP 583 Differentiation of Human Brain Gliomas by Lipid Wuppertal, Germany Analysis Using Desorption Electrospray Ionization WP 574 Imaging of Neuropeptides with High Lateral Imaging Mass Spectrometry; Livia S. Eberlin 1; Resolution and High Mass Accuracy by Matrix Allison Dill1; Alexandra Golby2; Keith Ligon2; Justin Assisted Laser Desorption/Ionization Mass Wiseman3; Nathalie Agar2; R. Graham Cooks 1; 1Purdue Spectrometry (MALDI MS); Sabine Günther; Andreas University, West Lafayette, IN; 2Brigham & Women’s Roempp; Wolfgang Kummer; Bernhard Spengler; Justus Hospital, Harvard Medical School, Boston, MA; Liebig University, Giessen, Germany 3Prosolia, Inc., Indianapolis, IN WP 575 ImmunoImaging: Quantitative Investigation of Tissue-Less Tissue Imaging and Simplification of the Page S118 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS 1 MALDI SAMPLE PREPARATION: DEPOSITION AND MALDI-TOF and TOF/TOF; Witold M Winnik ; 2 1 1 SENSITIVITY, 584 - 602 Oscar Alzate ; Maribel Bruno ; Lyle Burgoon ; Yue Ge1; Gary R. Klinefelter1; Prasada R. Kodavanti1; WP 584 Investigating the Sensitivity of Laserspray Ionization 2 1 of an Orbitrap Exactive; Diana L. Sardelis; Catherine Jennifer B. Robinette ; Juan Suarez ; Kathleen Wallace1;1US EPA, NHEERL, Research Triangle Park, Bentzley; Charles N. Mcewen; University of the 2 Sciences in Philadelphia, Philadelphia, PA NC; UNC-Chapel Hill, Chapel Hill, NC WP 585 Sensitivity in MALDI with Small Spot Sizes; Andriy WP 599 High Resolution MALDI Plates for the Direct Yamchuck; Victor Spicer; Werner Ens; University of Coupling of PAGE Separations and Tissue Analysis Manitoba, Winnipeg, Canada with MALDI Mass Spectrometry; Stephen J. Hattan; WP 586 In-Capillary Protein Methyl Esterification at Sub- Kenneth C. Parker; Marvin L. Vestal; VIC Instruments Microliter Volumes; Ashley N. St. Pierre; Ken K.-C. Corporation, Sudbury, MA Yeung; The University of Western Ontario, London, WP 600 Evaluation and Optimization of Protein Recovery Canada from Gels for MALDI-MS Analysis; Kandalama WP 587 Nanoliter-Volume Protein Desalting and Enrichment Priyasantha; Mary Elizabeth Gimon-Kinsel; Gary R. with Capillary Isoelectric Trapping; Christina J Kinsel; Southern Illinois University Carbondale, Booker; Jose S. Mejia; Samuel Sun; Sarah Woolsey; Carbondale, Illinois Ken K.-C. Yeung; University of Western Ontario, WP 601 Rapid Automated Off-Line LC-MALDI Analyses London, Canada Using a Liquid Matrix Optimized for Uniformity and WP 588 Polymer Segregation in MALDI Spots Investigated Durability of Ionization; Yuko Fukuyama; Shinichi by MALDI-TOF Imaging MS; Steffen M. Weidner; Iwamoto; Natsumi Funakoshi; Kenichi Taniguchi; Katrin Hoffmann; Andreas Thuenemann; Jana Koichi Tanaka; Shimadzu Corporation, Kyoto, Japan Falkenhagen; Fed.Inst.f.Mat.Research, Berlin, Germany WP 602 Polymer-Based Single-Use Targets for Protein Characterization for NanoLC-MALDI Mass WP 589 Solid State Nuclear Magnetic Resonance as a Tool to 1 2 Explore MALDI Samples; Caroline Barrere1; Hélène Spectrometry; Wolfgang Winkler ; Werner Balika ; 1 2 3, 4 Manfred Koranda2; Guenter Allmaier1; 1Vienna Pizzala ; Michael Mazarin ; Fabio Ziarelli ; Laurence 2 Charles 5; 1University Aix-Marseille, Marseille, France; University of Technology, Vienna, Austria; Sony DADC 2TOTAL FRANCE - CReS, Solaize, France; 3Sciences Austria AG, Anif, Austria Chimiques Federation–CNRS, Marseille, France; ION SOURCES, API, 603 - 611 4Spectropole, Marseille, France; 5University Aix- WP 603 Cluster Compositions of Acetonitrile and Marseille I & III, Marseille, France Acetonitrile-Water Studied by Liquid-Ionization WP 590 Detection of Monolayer/Multilayer Transition at MS/MS and Optimized Structures by Computer Surfaces by Laser-Desorption Mass Spectrometry; Calculation; Haruhiko Fukaya1; Yasuo Shida1; Hans Joachim Räder; Nataliya Mavrinskaya; Klaus Masahiko Tsuchiya2; 1Tokyo University of Pharmacy Müllen; MPI for Polymer Research, Mainz, Germany and Life Science, Tokyo, Japan; 2Yokohama National WP 591 Sublimed MALDI-Matrix Supports Large University, Yokohama, Japan Biomolecule Detection and High Density Arrays with WP 604 Laser Ablation Electrospray Ionization Mass SAMDI-TOF MS; Alex Swaim1; Daniel Smith2; Jaekuk Spectrometry for Phospholipids and Small Molecules Kim2; Steven Patrie2; 1UT Dallas, Kingwood, TX; 2UT in Tissue Samples; Berk Oktem; Eugene Moskovets; Southwestern Medical Center, Dallas, TX Vladimir M. Doroshenko; MassTech Inc., Columbia, WP 592 New Frontiers in MALDI-TOF-MS Sample MD Preparation for Peptides Analysis Using Electric WP 605 Driving Atmospheric Pressure Ion Sampling Field Enhanced Deposition; Parul Jain; Julie Harmon; Efficiency to the MAX; Alex Mordehai1; Paul Momoh1; University of South Florida, Tampa, FL Michael Ugarov 1; Anabel Fandino1; Gangqiang Li2; WP 593 High Intra- and Inter-Sample Precision Obtained for Stuart Hansen2; Peter Williams 2; John Fjeldsted1; Electrospray Deposited MALDI TOFMS Samples of 1Agilent Technologies, Santa Clara, CA; 2Agilent Controlled Spot Size; Jonathan Haulenbeek; Kevin G. Laboratories, Santa Clara, CA Owens; Drexel University, Philadelphia, PA WP 606 Modular Computational Toolset for Atmospheric WP 594 RF Plasma Polymerized Ethylenendiamine Modified Pressure Ionization Method Development: SIMION MALDI Target for Enhanced E. coli Proteomic Meets FEM; Walter Wissdorf1; Larissa Pohler1; Analysis; Lijuan Peng 1; Gary R. Kinsel1; 1Southern Thorsten Pöhler2; Herwart Hönen2; Klaus J. Illinois University, Carbondale, IL Brockmann1; Thorsten Benter2; 1University of WP 595 Chip-Based Open Channel Preconcentration for Wuppertal, Wuppertal, Germany; 2RWTH Aachen, Mass Spectrometry Analysis of Biosamples; Johan Aachen, Germany Jacksén; Åsa Emmer; Royal Institute of Technology, WP 607 Novel Laminar Flow Ion Sources for LC- and GC- Stockholm, Sweden API MS; Ian Barnes1; Hendrik Kersten1; Walter WP 596 The Need for Constant Review of Laboratory Wissdorf1; Thorsten Pöhler2; Herwart Hönen2; Sonja Consumables; Celia Smith; Rainer Cramer; University Klee1; Klaus J. Brockmann1; Thorsten Benter1; of Reading, Reading, UK 1University of Wuppertal, Wuppertal, Germany; 2RWTH WP 597 Correlating Histochemistry and Mass Spectrometry Aachen, Aachen, Germany for Single Cell-Type Analysis: To Stain or Not to WP 608 A Comparison of the Performance of Diode Pumped Stain; Jamie Allen1; Erin Seeley1; Kristina Solid State Lasers and Excimer Lasers in LC- and Schwamborn1; Ray Mernaugh1; Richard M. Caprioli2; GC-APLI MS; Sonja Klee; Hendrik Kersten; Matthias 1Vanderbilt University, Nashville, TN; 2Vanderbilt Univ Lorenz; Walter Wissdorf; Klaus J. Brockmann; Thorsten Sch of Med, Nashville, TN Benter; University of Wuppertal, Wuppertal, Germany WP 598 Improved Method for Identification of Low WP 609 Fundamental Characterization of Ion Transfer Abundance Proteins Using 2D-gel Electrophoresis, Capillaries Used in Atmospheric Pressure Ionization 58th ASMS Conference on Mass Spectrometry Page S119 WEDNESDAY POSTERS Sources; Klaus J. Brockmann; Walter Wissdorf; Lukas WP 623 A New Method To Measure Relative Collision Cross Hyzak; Hendrik Kersten; Thorsten Benter; University of Sections Using Fourier Transform Ion Cyclotron Wuppertal, Wuppertal, Germany Resonance Mass Spectrometry; Fan Yang; David V. WP 610 Spatially and Temporally Resolved Atmospheric Dearden; Brigham Young University, Provo, UT Pressure Laser Ionization as a Powerful Tool for the WP 624 First and Second Hydration Shell Energies of Barium Characterization of Ion Sources: An Overview; Dications; Oscar Wheeler; Theresa Cooper; Peter B. Matthias Lorenz; Walter Wißdorf; Sonja Klee; Hendrik Armentrout; University of Utah, Salt Lake City, Utah Kersten; Klaus J. Brockmann; Thorsten Benter; WP 625 Transition Metal Hydration Studies: Experimental University of Wuppertal, Wuppertal, Germany and Theoretical Investigation of Binding Energies for 2+ WP 611 Progress in the Development and Characterization of the Cu (H2O) n, n=7-10 Complexes; Andrew In-source Atmospheric Pressure-Electron Capture Sweeney; Theresa Cooper; Peter B. Armentrout; Dissociation (AP-ECD); Damon Robb; Jason Rogalski; University of Utah, Salt Lake City, UT Juergen Kast; Michael Blades; University of British WP 626 ESI-MS Estimation of the Acidities of Polyfunctional Columbia, Vancouver, BC Groups by the Kinetic Method: Hydroxycinnamic ION STRUCTURES/ENERGETICS II, 612 - 636 Acids; Juan Davalos; Andres Guerrero; Antonio Chana; WP 612 Mass Spectrometry Study of Cobalt(III) Porphyrins Rebeca Herrero; Instituto de Quimica-Fisica Fragmentation Patterns and Their Ligand Binding Rocasolano-CSIC, Madrid, Spain Strength; Ekta Mishra1; Victor Ryzhov1; Jill L. WP 627 Experimental and Computational Studies of Gas- Worlinsky2; Christian Brückner2; 1Northern Illinois Phase Acidities Related to Deprotonation of University, Dekalb, IL; 2University of Connecticut, Tripeptide Backbones Using FT-ICR Mass Storres, CT Spectrometry; Samantha Bokatzian-Johnson; John WP 613 Reinvestigation of the Thermochemistry of Sulfuric Killian; Myrna Hernandez-Matus; David Dixon; Carolyn Acid-Related Ions; Sara Koepke; Thomas M. Gilbert; J. Cassady; University of Alabama, Tuscaloosa, AL Lee S. Sunderlin; Northern Illinois University, DeKalb, WP 628 Experimental Determination of the Gas-Phase Heat Illinois of Formation of 2,3 and 3,4-Pyridyne; Nathan Rau; WP 614 Thermochemical Properties of Fluorinated Alanes; Paul Wenthold; Purdue University, Lafayette, IN Jamelle K.P. Williams; Paul G. Wenthold; Purdue WP 629 Polar Groups Induced Acidity Enhancements of Carboxylic Acids in the Gas Phase and in Solution; University, West Lafayette, IN 1 1 1 WP 615 Investigation of the O-O Dissociation Energy of Yuan Tian ; Jianhua Ren ; University of the Pacific, Peroxycarboxyl Anions; Alex A. Nickel; Jerry G. Stockton, CA Lanorio; Kent M. Ervin; University of Nevada, Reno, WP 630 Gas Phase Acidities of Short Cysteine-Containing Reno, NV Peptides; Jialin Shen; Jianhua Ren; University of the WP 616 Collision-Induced Dissociation of Protonated and Pacific, Stockton, CA Group I Metallated Lysine Complexes: Binding WP 631 Proton Affinities of Charged Peptides; Xiaoning Zhao; Energies, Transition State Energies, and Theoretical Noah Fang; Jianhua Ren; Uinversity of the Pacific, Structures; Amy A. Clark; Peter B. Armentrout; Stockton, CA WP 632 Gas-Phase Proton Affinity of N-heterocyclic University of Utah, Salt Lake City, UT 1 1 1 WP 617 Group I Metal – Ligand Thermochemistry of N- Carbenes (NHCs); Min Liu ; Jeehiun K. Lee ; Rutgers Methyl Proline (NMP) – An Experimental and University, Piscataway, NJ Theoretical Approach; Abhigya Mookherjee; Peter WP 633 Investigation of Nanoparticle Surface Chemistry for Armentrout; University of Utah, Salt Lake City, UT Laser Desorption Ionization Mass Spectrometry WP 618 Gas-Phase Acidities and Mass Spectral Applications; Mario E. Gomez; Edward T. Castellana; Fragmentation Processes of Anions from N- David H. Russell; Texas A&M University, College Benzoylamino Acids; Robert L. White; J. Stuart Station, TX Grossert; Lana E. Greene; Dalhousie University, WP 634 Synthesis and Enhanced Reaction Selectivity of the í; Department of Chemistry, Halifax, Canada Mixed-Metal RCCMgLiCl3 ; George N. Khairallah; WP 619 Structures and Energetics of [UracilnCa] 2+ (n=14-4) Edward Whiting; Charlene C. L. Thum; Richard A. J. Clusters by BIRD and Theoretical Studies; Elizabeth O'hair; Bio21 Inst,Univ of Melbourne, Melbourne, A.L. Gillis; Travis D. Fridgen; Memorial University of Australia Newfoundland, St. John's, Newfoundland and Labrador, WP 635 Charge State Stability of PAMAM Dendrimers in the 1 2 3 Canada Gas Phase; Aura Tintaru ; Sabrina Pricl ; Jiangyu Wu ; 3 1 1 WP 620 Energetics of Cation-Phosphate Interactions in Ling Peng ; Laurence Charles ; Aix-Marseille 2 Biological Systems; Julia Laskin1; Zhibo Yang 2; Amina University, Marseille, France; University of Trieste, 3 S. Woods3; 1Pacific NW National Laboratory, Richland, Trieste, Italy; Université de la Méditeranée, Marseille, WA; 2University of Colorado, Boulder, CO; 3NIDA IRP, France NIH, Baltimore, MD WP 636 An ab-initio Study on Guanidination of Peptides in WP 621 Complexation of Į,ȧ-alkyldiammonium Ions with Matrix-Assisted Laser Desorption Ionization; 1 3 Cucurbit[7]uril in the Gas Phase: Dependence of Francesco L Brancia ; Roberto Marchese ; Mauro 2 3 1 Bond Strength on Alkyl Chain Length; Daniel Stener ; Simone Raugei ; Aarhus University, Tjele, 2 3 Mortensen; David V. Dearden; Brigham Young Denmark; University of Trieste, Trieste, Italy; SISSA, University, Provo, UT Trieste, Italy WP 622 Influence of Weak Interactions on Supramolecular ION MOBILITY II, 637 - 665 Binding: Characterization of Cucurbit[6]uril WP 637 Improving the Resolution of Ion Mobility Complexes with Alkylmonoammonium Ions via Spectrometry; Glenn E. Spangler; Technispan LLC, Experiment and Theory; Ruijun Shi; David V. Lutherville, MD Dearden; Brigham Young University, Provo, UT Page S120 58th ASMS Conference on Mass Spectrometry WEDNESDAY POSTERS WP 638 New Ion Mobility Spectrometry with a novel Flow- WP 649 Assembly Pathways of Islet Amyloid Polypeptide and Rectification Nozzle for Mass Spectrometry; Masaru Aggregation Inhibition with a Designed Peptide, Nishiguchi; Yoshihiro Ueno; Fujio Inoue; Daisuke IAPP-GI; Nicholas F. Dupuis; Chun Wu; Michael T. Okumura; Hiroaki Waki; Hiroto Itoi; Shimadzu Bowers; University of California, Santa Barbara, CA Corporation, Kyoto, Japan WP 650 Collision Cross-Section Determination of Isomeric WP 639 Development of a “Ping-Pong” Drift Tube: Carotenoids Using Electrospray Ion Mobility Time- Theoretical Considerations of Resolving Power; of-Flight Mass Spectrometry; Linlin Dong 1; Henry Y. Sunyoung Lee; Stephen Valentine; David E. Clemmer; Shion 2; Roderick Davis3; Brent Terry-Penak1; Richard Indiana University, Bloomington, IN B. van Breemen1; 1University of Illinois College of WP 640 Conformational Studies of Melittin Using Pharmacy, Chicago, IL; 2Waters Corp., Milford, MA; Electrospray Ion Mobility-Mass Spectrometry: The 3Univ.of Illinois at Chicago Research Resources Ctr, Effects of Temperature and Solvent Composition; Chicago, IL Sevugarajan Sundarapandian1; Jody May1; Brandon T. WP 651 Bottom Up Approaches for Protein Characterization Ruotolo2; David H. Russell3; John A. Mclean1; by Laserspray Ionization – Ion Mobility 1Department of Chemistry, Vanderbilt University, Spectrometry – Mass Spectrometry; Darrell Marshall; Nashville, TN; 2Department of Chemistry, University of Thushani N. Herath; Ellen D. Inutan; Sarah Trimpin; Michigan, Ann Arbor, MI; 3Department of Chemistry, Wayne State University, Detroit, MI Texas A&M University, College Station, TX WP 652 Characterization of Proteins by Laserspray WP 641 Simulation of Ion Mobility at Reduced Pressures Ionization-Ion Mobility Spectrometry-Mass Based on Revised Ion-Molecule Collision Models; Spectrometry; Fadia Cudry; Ellen D. Inutan; Tamara Dimitris Papanastasiou1; Mikhail Sudakov2; Roger Hendrickson; Sarah Trimpin; Wayne State University, Giles3; Emmanuel Raptakis1; 1Fasmatech, Athens, Detroit, MI Greece; 2Ryazan State Radioengineering University, WP 653 Folding Analysis of Carbonic anhydrase2 Using Ion Ryazan, Russia; 3Shimadzu Research Laboratory, Mobility and Subsequent Collision-Induced Manchester, UK Dissociation; Kenji Hirose 1; Yoshiaki Nabuchi2; Mitsuo WP 642 Protein Complexes as Ion Mobility Standards: Takayama2; 1Nihon Waters K.K., Osaka, Japan; Strategies for Reducing Their Uncertainties and 2Yokohama City University, Yokohama, Japan Benefits for Gas-Phase Structural Biology; Zoe Hall1; WP 654 Laserspray Ionization (LSI) Ion Mobility Kevin Giles2; John B. Hoyes2; Matthew F. Bush1; Carol Spectrometry (IMS) Mass Spectrometry (MS) of V. Robinson1; 1Department of Chemistry, University of Proteins; Ellen D. Inutan; Sarah Trimpin; Wayne State Oxford, Oxford, UK; 2Waters Corporation, Manchester, University, Detroit, MI UK WP 655 Higher Resolving Power and Ion Mobility WP 643 Characterization of Insulin Oligomers Using Ion Significantly Reduces the Effects of Over Lapping Mobility Mass Spectrometry; Rune Salbo1, 2; Ingrid Ion Clusters in "Systems" Samples; Scott Geromanos; Pettersson1; Helle Naver1; Thomas J.D. Jørgensen2; Kim Dan Golick; Marc V. Gorenstein; Jim Langridge; Waters F. Haselmann1; 1Novo Nordisk, Måløv, Denmark; 2BMB, Corporation, Milford, MA University of Southern Denmark, Odense, Denmark WP 656 MALDI Ion Mobility-Mass Spectrometry for Post- WP 644 Determining the Topological Arrangements of Ionization Fractionation of Peptides Containing Protein Complexes by Combining Ion Mobility Mass Posttranslational Modifications; Whitney B. Ridenour; Spectrometry and Molecular Modelling; Argyris John A. Mclean; Kevin L. Schey; Richard M. Caprioli; Politis1, 2; Ahyoung Park 1, 2; Suk-Joon Hyung2, 3; Daniel Vanderbilt University, Nashville, TN Barsky2, 4; Brandon Ruotolo2, 3; Carol V. Robinson1, 2; WP 657 High-Throughput Ion Mobility-Mass Spectrometry 1University of Oxford, Oxford, UK; 2University of Strategies for Monitoring Cellular Response in Real Cambridge, Cambridge, UK; 3University of Michigan, Time; Jody C. May; Jeffrey R. Enders; Sevugarajan Ann Arbor, MI; 4Lawrence Livermore National Sundarapandian; Kevin T. Seale; John P. Wikswo; John Laboratory, Livermore, CA A. Mclean; Vanderbilt University, Nashville, TN WP 646 Traveling-Wave Ion Mobility Mass Spectrometry WP 658 Use of Traveling-Wave Ion Mobility Mass Studies of Gas-Phase Conformations of CC and CXC Spectrometry to Analyze Dead-End Isomers from Chemokines: Monocyte Chemoattractant Protein-1,- Peptide Cross-Linking: Application to Lys Intrinsic 2, and Interleukin-8; Zhiyin Xun; Julie A. Leary; UC Reactivity; Fabio C Gozzo1; Luiz Fernando Arruda Davis, Davis, CA Santos 1; Amadeu H Iglesias2; Eduardo J Pilau 1; WP 647 Development of a Hofmeister Series Analogue for Alexandre F. Gomes1; 1IQ - University of Campinas, Gas-phase Protein Complexes: Evidence from Ion Campinas, Brazil; 2Brazilian Synchrotron Light Source, Mobility-Mass Spectrometry; Suk-Joon Hyung; Han Campinas, Brazil Linjie; Alex Pagliaro; Brandon Ruotolo; University of WP 659 Ion Mobility Analyses of Peptide Ions Produced By Michigan, Ann Arbor, MI CID and ETD Fragmentation; Ross Chawner1; Jeff WP 648 Ion-Mobility Mass Spectrometry Enables the Brown 2; Kevin Giles2; Simon J. Gaskell3; Claire Eyers1; Structure Determination of Heterogeneous Protein 1University of Manchester, Manchester, UK; 2Waters Assemblies; Justin Benesch; University of Oxford, Corporation, Manchester, UK; 3Queen Mary, University Oxford, UK of London, London, UK WP 648 New Avenues in Natural Product Discovery Using WP 660 Separation of Fragment Ions of Lipids in Gaucher Ion Mobility-Mass Spectrometry; Cody Goodwin; Disease Tissues Using High/Low Energy Function Larissa S. Fenn; Brian O. Bachmann; John A. Mclean; Switching MALDI IMS Imaging; Marten Snel1; Paul J Vanderbilt University Department of Chemistry, Trim1; Emmanuelle Claude2; Jim Langridge2; Maria Nashville, TN Fuller1; 1Lysosomal Diseases Research Unit, SA 58th ASMS Conference on Mass Spectrometry Page S121 WEDNESDAY POSTERS Pathology, North Adelaide, Australia; 2Waters WP 672 A New Sample Introduction Technique for Mass Corporation, Manchester, UK Spectrometry That Allows for Dried Blood Spot WP 661 Investigating Aniline Isomer Separation Utilizing Analysis and Online SPE; Lena Knegt; Emile Koster; Travelling Wave Ion Mobility and Polarizable Drift Spark Holland, Emmen, Netherlands Gases; Gustavo HMF Souza1, 3; Iain D G Campuzano1, 3; WP 673 Laminar Flow Assisted Ion Transfer for Mass Priscila M Lalli2; Fabiane M Nachtigall2; Maria Analysis; Sandilya Garimella; Jason Harper; Guangming Francesca Riccio 2; Gilberto de Sa2; Romeu J Daroda2; Huang; Wei Xu; R. Graham Cooks; Zheng Ouyang; Vanderlea de Souza2; Marcos N. Eberlin2; 1Waters Purdue University, West Lafayette, IN Corporation, Barueri, Sao Paulo, Brazil; 2ThoMSon WP 674 Characterization of Protein and Labile Protein Mass Spectrometry Laboratory, UNICAMP, Campinas, Modifications Using Laserspray Ionization on a LTQ Brazil; 3Waters Corporation, Manchester, UK - Electron Transfer Dissociation Mass Spectrometer; WP 662 Thermally-Assisted Extractive Electrospray Ion Thushani N. Herath; Darrell Marshall; Alicia Richards; Mobility Mass Spectrometry Characterisation of Ellen D. Inutan; Sarah Trimpin; Wayne State Univ., Medicinal Spray Formulations; James Scrivens; Detroit, MI Charlotte Scarff; Jonathon Snelling; Univ of Warwick, WP 675 Determination of 41Ca Using the Isobar Separator Coventry, UK for Anions Coupled to an Accelerator Mass WP 663 Intrinsic Mobility of Gaseous Cationic and Anionic Spectrometer; Jean-Francois Alary1; Lisa M. Cousins 2; Aggregates of Ionic Liquids by Ion Mobility Mass John Eliades3; Reza Javahari2; William E. Kieser4; Spectrometry; Priscila M Lalli1; Yuri E Corilo1; Albert E. Litherland3; Sha Ye2; Xiao-Lei Zhao3; Gilberto Sa2; Romeu J Daroda2; Vanderlea Souza2; 1Isobarex Corp., Bolton ON, Canada; 2Ionics Mass Gunter Ebeling3; Jairton Dupont3; Marcos N Eberlin1; Spectrometry Group Inc., Bolton ON, Canada; 1ThoMSon Mass Spectrometry Laboratory, UNICAMP, 3IsoTrace, University of Toronto, Toronto ON, Canada; Campinas, Brazil; 2National Institute of Metrology, 4University of Ottawa, Ottawa ON, Canada Inmetro, Duque de Caxias, RJ, Brazil; 3Laboratory Of WP 676 Withdrawn Molecular Catalysis, UFRGS, Porto Alegre, Brazil WP 677 Chip-Based Electrospray Interface for nanoLC-MS; WP 664 Cage Flight! Ion Mobility-Mass Spectrometry of Self- Don Arnold; Remco Van Soest; David Neyer; J. Bryce Assembling 3-D Architectures.; Martin De Cecco1; Young; Nicole Hebert; Eksigent Technologies, Dublin, Chris Moffat1; Nick Tomczyk2; Sarah Pike1; Paul CA Lusby1; Perdita Barran1; 1University of Edinburgh, WP 678 Interfacing Ion Funnel Trap (IFT) to Triple Edinburgh, UK; 2Waters Corporation, Manchester, UK Quadrupole Analyzer for Enhancing Sensitivity in WP 665 Characterization of Self-Assembled Zinc Terpyridine Multiple Reaction Monitoring (MRM) Mode.; Macrocycles Using Traveling Wave Ion Mobility Satendra Prasad; David Prior; Bill Danielson; Karl Mass Spectrometry; Xiaopeng Li; Yi-Tsu Chan; Weitz; Yehia Ibrahim; Richard D. Smith; Mikhail Belov; George Newkome; Chrys Wesdemiotis; The University Pacific Northwest National Laboratory, Richland, WA of Akron, Akron, OH WP 679 Optimization of the Electrodynamic Ion Funnel for INSTRUMENTATION: NEW CONCEPTS I, 666 - 680 Enhanced Low Mass Transmission: Influence of WP 666 Yttria / Rhenium Alloy Emission Filaments for Mass Funnel Operational Pressure on Ion Transmission; Spectrometers; John Manura; Ronald Shomo; Paul Momoh; Michael Ugarov; Mark Werlich; Tom Christopher W. Baker; Scientific Instrument Services, Knotts; Alex Mordehai; Agilent Technologies, Santa Ringoes, NJ Clara, CA WP 667 Electrospray Ionization Mass Spectrometry from WP 680 A Novel Low Power, Real Time Pre-Concentrator for Discrete Nanoliter-Sized Droplets; Patrik Ek; Mårten Use in Laboratory and Field Portable Chemical Detection Systems; Thomas Bowden1; Aaron Jesseph2; Stjernström; Åsa Emmer; Johan Roeraade; KTH Royal 1 1 1 Institute of Technology, Stockholm, Sweden Pedro Ojeda ; David Rafferty ; Abrar Riaz ; Michael Spencer1; Guido F. Verbeck2; James Wylde1; 11st Detect WP 668 Microfabrication of On-Line Micro-Reactors for Gas 2 Chromatography Isotope Ratio Mass Spectrometry Corp., Houston, TX; University of North Texas, Denton, (GC-IRMS); Herbert Tobias; J Thomas Brenna; Cornell TX University, Ithaca, NY WP 669 A Pulsed-Valve Mass Spectrometric Approach to Measuring Trace Gas Permeation at Ambient Atmospheric Conditions; Dana Reed; Fred DeRoos; Mark Mueller; Mark Roehrig; 3M Corporation, St. Paul, MN WP 670 Novel DMS/MS Coupling for a Triple Quadrupole Mass Spectrometer; Bradley B Schneider1; Thomas Covey1; Stephen L Coy2; Evgeny V Krylov2; Erkinjon Nazarov2; 1AB SCIEX, Concord, Canada; 2Sionex Corp., Bedford, MA WP 671 Analyzing Liquids via Direct Injection Proton- Transfer-Reaction Mass Spectrometry (PTR-MS); Simone Jürschik1; Philipp Sulzer1; Lukas Maerk 1; Alfons Jordan1; Ralf Schottkowsky1; Eugen Hartungen1; Gernot Hanel1; Hans Seehauser1; Stefan Jaksch1; Stefan Haidacher1; Tilmann D. Märk1, 2; 1IONICON Analytik, Innsbruck, Austria; 2Institut für Ionenphysik und Angewandte Physik, Innsbruck, Austria