NUB1 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science NUB1 Rabbit pAb Catalog No.: A18665 Basic Information Background Catalog No. This gene encodes a protein that functions as a negative regulator of NEDD8, a A18665 ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 Observed MW conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the 71kDa proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and Calculated MW mutations in that gene can abolish interaction with this protein, which may contribute to 69kDa/70kDa the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions Category in multiple system atrophy, with this abnormal accumulation being specific to alpha- synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript Primary antibody variants. Applications WB, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 51667 Q9Y5A7 IF 1:50 - 1:200 Immunogen Recombinant protein of human IFT140. Synonyms NUB1; BS4; NUB1L; NYREN18; NEDD8 ultimate buster 1 Contact Product Information 400-999-6126 Source Isotype Purification Rabbit IgG Affinity purification [email protected] www.abclonal.com.cn Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using NUB1 Rabbit pAb (A18665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 300s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Identification of the Binding Partners for Hspb2 and Cryab Reveals
Brigham Young University BYU ScholarsArchive Theses and Dissertations 2013-12-12 Identification of the Binding arP tners for HspB2 and CryAB Reveals Myofibril and Mitochondrial Protein Interactions and Non- Redundant Roles for Small Heat Shock Proteins Kelsey Murphey Langston Brigham Young University - Provo Follow this and additional works at: https://scholarsarchive.byu.edu/etd Part of the Microbiology Commons BYU ScholarsArchive Citation Langston, Kelsey Murphey, "Identification of the Binding Partners for HspB2 and CryAB Reveals Myofibril and Mitochondrial Protein Interactions and Non-Redundant Roles for Small Heat Shock Proteins" (2013). Theses and Dissertations. 3822. https://scholarsarchive.byu.edu/etd/3822 This Thesis is brought to you for free and open access by BYU ScholarsArchive. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of BYU ScholarsArchive. For more information, please contact [email protected], [email protected]. Identification of the Binding Partners for HspB2 and CryAB Reveals Myofibril and Mitochondrial Protein Interactions and Non-Redundant Roles for Small Heat Shock Proteins Kelsey Langston A thesis submitted to the faculty of Brigham Young University in partial fulfillment of the requirements for the degree of Master of Science Julianne H. Grose, Chair William R. McCleary Brian Poole Department of Microbiology and Molecular Biology Brigham Young University December 2013 Copyright © 2013 Kelsey Langston All Rights Reserved ABSTRACT Identification of the Binding Partners for HspB2 and CryAB Reveals Myofibril and Mitochondrial Protein Interactors and Non-Redundant Roles for Small Heat Shock Proteins Kelsey Langston Department of Microbiology and Molecular Biology, BYU Master of Science Small Heat Shock Proteins (sHSP) are molecular chaperones that play protective roles in cell survival and have been shown to possess chaperone activity.
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Regulation of NUB1 Activity Through Non-Proteolytic Mdm2-Mediated Ubiquitination
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1 Negative Regulator of Ubiquitin-Like Protein 1 Modulates the Autophagy-Lysosomal Pathway Via P62 to Facilitate the Extracellul
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Regulation of NUB1 Activity Through Non- Proteolytic Mdm2-Mediated Ubiquitination
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NYREN18 (NUB1) Mouse Monoclonal Antibody [Clone ID: OTI2G1] Product Data
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Datasheet: VMA00450KT Product Details
Datasheet: VMA00450KT Description: NUB1 ANTIBODY WITH CONTROL LYSATE Specificity: NUB1 Format: Purified Product Type: PrecisionAb™ Monoclonal Clone: 4H2 Isotype: IgG2a Quantity: 2 Westerns Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Western Blotting 1/1000 PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type. Target Species Human Species Cross Reacts with: Mouse Reactivity N.B. Antibody reactivity and working conditions may vary between species. Product Form Purified IgG - liquid Preparation 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites Buffer Solution Phosphate buffered saline Preservative 0.09% Sodium Azide (NaN3) Stabilisers 1% Bovine Serum Albumin 50% Glycerol Immunogen Recombinant protein fragment corresponding to aa 1-326 of human NUB1 (NP_057202) produced in E.coli External Database Links UniProt: Q9Y5A7 Related reagents Entrez Gene: Page 1 of 3 51667 NUB1 Related reagents Synonyms NYREN18 Specificity Mouse anti Human NUB1 antibody recognizes the NUB1, also known as negative regulator of ubiquitin-like proteins 1, NEDD8 ultimate buster-1, BS4, renal carcinoma antigen NY-REN-18 or simply NYREN18, .
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