DOCSLIB.ORG

MOLECULAR and CELLULAR BIOLOGY of HELMINTH PARASITES V Burroughs Wellcome Fund

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
MOLECULAR and CELLULAR BIOLOGY of HELMINTH PARASITES V Burroughs Wellcome Fund MOLECULAR AND CELLULAR BIOLOGY OF HELMINTH PARASITES V BRATSERA HOTEL, HYDRA, GREECE 12-17 SEPTEMBER 2008 ~~~~~~~~~~~~ This meeting was made possible through the generous support of: Burroughs Wellcome Fund SCIENTIFIC PROGRAMME All timings include minimum of 5 minutes for discussion. ~~~~~~~~~~~~~~~~~~~~ ~ Friday 12 September ~ 15:00 - 18:00 Registration, Bratsera Hotel 18:15 Introduction: Rick Maizels, University of Edinburgh 18:45 Plenary Lecture: Paul Sternberg, California Institute of Technology Nematode genomics and informatics: leveraging C. elegans 20:00 Welcome Reception, Bratsera Hotel Day 1 ~Saturday 13 September ~ GENOMES AND GENETICS Session 1: GENOMICS AND EVOLUTION OF PARASITISM Chair - Murray Selkirk, Imperial College London 9:00 Matthew Berriman, Welcome Trust Sanger Institute. Parasitic helminth genome sequencing 9:40 Juan Pedro Laclette, Universidad Nacional Autonoma. The genome project of Taenia solium 10:00 David Bird, NC State University: Sequence of Meloidogyne hapla. A compact nematode genome for plant parasitism 10:20 Sommer Ralf, Max-Planck Institute. The genome of Pristionchus pacificus and implications for the evolution of parasitism Coffee Break Session 2: CROSSOVER FROM C ELEGANS TO PARASITES Chair - Paul Sternberg, California Institute of Technology 11:10 Adrain Streit, Tübingen. Genetics in the parasitic nematode genus Strongyloides 11:30 John Gilleard, Faculty of Veterinary Medicine. Developing Haemonchus contortus as a model parasite for forward genetic studies of anthelmintic resistance 11:50 Collette Britton, University of Glasgow. Expression of potential control targets of parasitic nematodes using Caenorhabditis elegans 12:10 Peter Geldhof, Ghent University. Functional analysis of Ostertagia ostertagi vaccine candidates in Caenorhabditis elegans 12:30 Claudia Welz, Univ of Vet Med Hannover. Functional expression of parasitic SLO-1 in Caenorhabditis elegans slo-1 knockout mutants Lunch/Free Time Session 3: TRANSFECTION IN PARASITIC HELMINTHS Chair - Eileen Devaney, University of Glasgow 16:30 James Lok, University of Pennsylvania. Transgenesis in Strongyloides stercoralis and S. ratti: prospects for transposon-mediated gene transfer and applications in studies of gene function, immunology and sensory neurobiology 17:10 Michelle Castelletto, University of Pennsylvania. Regulation and function of fktf-1 in transgenic Strongyloides stercoralis 17:30 Thomas Unnasch, University of South Florida. Analysis of transcription and mRNA processing in Brugia malayi using a homologous transient transfection system 17:50 Paul Brindley, George Wash Med Center. Integration of reporter transgenes into Schistosoma mansoni chromosomes mediated by pseudotyped murine leukemia virus 18:10 - 20:00 Poster Session 1 SCIENTIFIC PROGRAMME ~ FRIDAY 12 SEPTEMBER AND SATURDAY 13 SEPTEMBER Day 2 ~ Sunday 14 September ~ MOLECULAR AND DEVELOPMENTAL BIOLOGY Session 4 DEVELOPMENT AND DIFFERENTIATION Chair - Ed Pearce, University of Pennsylvania 9:00 Klaus Brehm, University of Würzburg. Cestode stem cells: their role in host-induced larval development and their utilization to achieve parasite transgenesis 9:40 Christoph Grevelding, Institute for Parasitology. Signaling molecules involved in gonad differentiation of Schistosoma mansoni 10:00 Olson Peter, Nat History Museum. Developmental genes in the life cycle of a parasitic flatworm 10:20 Svenja Beckmann, Justus-Liebig-University. The Syk-kinase SmTK4 from Schistosoma mansoni: upstream interaction partners and functional aspects in oogenesis and spermatogenesis Coffee Break Session 5 HELMINTH GENE FAMILIES AND SIGNALLING Chair - Charles Shoemaker, Tufts University 11:10 Cecilia Fernández, Universidad de la República. A diverse family of Kunitz inhibitors from Echinococcus granulosus involved in host-parasite cross-talk in echinococcosis 11:30 Bernadette Connolly, University of Aberdeen. TGF-beta and hedgehog signalling pathways in Trichinella spiralis 11:50 Ed Pearce, University of Pennsylvania. TGFbeta signaling in schistosomes 12:10 Sally Williamson, University of Bath. The nicotinic acetylcholine receptors of Ascaris suum 12:30 Niki Gounaris, Imperial College London. Secreted proteins of Trichinella spiralis modulate purinergic signalling in immune cells Lunch/Free Time (note earlier start time for session 6 to allow for sunset dinner) Session 6 MOLECULAR MECHANISMS IN HELMINTHS Chair - Tom Unnasch, University of South Florida 15:30 Alan Scott, Johns Hopkins University. The Brugia malayi genome 16:10 Larry McReynolds, New England Biolabs. miRNAs in Brugia malayi 16:30 Murray Selkirk, Imperial College London. RNAi in parasitic nematodes 16:50 Richard Davis, Univ of Colorado. RNA metabolism and molecular adaptation in Ascaris embryos 18:30 ~ Boats depart from Hydra port for Vlychos 19:00 Dinner, Vlychos Taverna SCIENTIFIC PROGRAMME ~ DAY 2 ~ SUNDAY 14 SEPTEMBER Day 3 ~ Monday 15 September ~ METABOLISM AND DRUG DISCOVERY Session 7 INSIGHTS FROM C ELEGANS Chair - Niki Gounaris, Imperial College London 9:00 Nektarios Tavernarakis, Foundation for Research and Technology - Hellas. Cellular energy metabolism and ageing in Caenorhabditis elegans 9:40 Richard Martin, Iowa State University. Levamisole channel currents and resistance in adult Caenorhabditis elegans muscle 10:00 Carolyn Behm, Australian National University. MITR-1: a conserved novel nematode-specific protein required for mitochondrial respiration in C. elegans 10:20 Anthony Page, University of Glasgow. A hypodermally expressed Haem peroxidase is critical for viability and cuticle formation in C. elegans Coffee Break Session 8 NEW DRUGS AND DRUG TARGETS Chair - Carolyn Behm, Australian National University 11:10 Pascal Mäser, University Of Bern. Molecular mechanisms of drug resistance in Haemonchus contortus 11:30 Achim Hoerauf, Univ Bonn Med Center. Anti-wolbachial chemotherapy of onchocerciasis - a macrofilaricide at last? 11:50 Mark Taylor, Liverpool School of Tropical Med. Filarial Wolbachia lipoprotein stimulates innate and adaptive inflammatory responses 12:10 Robert Greenberg, University of Pennsylvania. Praziquantel-dependent effects on expression of a P-glycoprotein homolog in Schistosoma mansoni 12:30 David Williams, Illinois State University. Identification of new drug leads targeting redox biochemistry for the control of schistosomiasis Lunch/Free Time Session 9 MOLECULAR DISCOVERY Chair - Alex Loukas, Queensland Inst of Med Research 16:30 Charles Shoemaker, Tufts University. Probing the helminth host-parasite inferface with phage-displayed antibodies 17:10 Patrick Skelly, Tufts Cummings School of Vet Med. Functional characterization of the schistosome surface 17:30 Eileen Devaney, University of Glasgow. Hsp90 and the biology of parasitism 17:50 Karl Hoffmann, Aberystwyth Univ. Transcriptomic analyses of schistosome biology: what we have learned and where we are headed 18:10 Richard Komuniecki, University of Toledo. Use of the Caenorhabditis elegans model system to identify the receptors in parasitic helminths modulating serotonin-stimulated paralysis 18:30 - 20:00 Poster Session 2 SCIENTIFIC PROGRAMME ~ DAY 3 ~ MONDAY 15 SEPTEMBER Day 4 ~ Tuesday 16 September ~ IMMUNOMODULATION AND IMMUNITY Session 10 MECHANISMS OF HOST IMMUNITY Chair - Rick Maizels, University of Edinburgh 9:00 Jonathon Ewbank, Centre d'Immunologie de Marseille-. Innate immunity in C. elegans 9:40 Rachel Lawrence, Royal Vet College. The mechanism by which mannose-binding lectin deficiency prevents parasite clearance and development of anti-parasite IgM 10:00 Nicola Harris, Swiss Federal Institute of Technology. Polyclonal and specific antibodies mediate protective immunity against enteric helminth infection 10:20 Graham LeGros, Malaghan Inst of Med Research The lung is a key site for protective immunity against gastrointestinal helminth parasites Coffee Break Session 11 HELMINTH IMMUNOMODULATION Chair - Achim Hoerauf, Univ Bonn Med Center 11:10 Helmut Haas, Borstel. S. mansoni egg excretory/secretory proteins - major modulators of the host’s immune response 11:30 Corinna Schnöller, Humboldt University. Gastrointestinal nematode infection inhibits experimental allergic airway inflammation but not atopic dermatitis 11:50 Matthew Taylor, University of Edinburgh. Induction and maintenance of effector and regulatory T cell responses during filarial infection 12:10 Sabine Specht, Univ Bonn Med Center. Murine filarial infection induces protection against malaria and inhibits allergic asthma 12:30 Rick Maizels, University of Edinburgh. Immunomodulation by H polygyrus products Lunch/Free Time Session 12 IMMUNE RECOGNITION AND VACCINES Chair - Graham LeGros, Malaghan Inst of Medical Research 16:30 Dave Knox, Moredun Research Institute. Proteomics and vaccine candidate discovery – the old and the new 16:50 Lustigman Sara, NY Blood Center. The immunopotentiating properties of the Onchocerca volvulus recombinant Ov-ASP-1 protein 17:10 Jan Bradley, University of Nottingham. The immunomodulatory properties of hOv-FAR 1; the fatty acid and retinol binding protein of Onchocerca volvulus 17:30 David Dunne, University of Cambridge. Human sensitization and desensitization to allergen- like proteins from Schistosoma mansoni depends on their expression patterns through the parasite life-cycle 17:50 Alex Loukas, Queensland Inst of Med Research. Apical membrane proteins as recombinant vaccines against Schistosoma mansoni - from men to mice then back to men 20:00 Farewell Dinner, Douskos Taverna, Hydra SCIENTIFIC PROGRAMME ~ DAY 4 ~ TUESDAY 16 SEPTEMBER POSTER
Load more

Recommended publications
  • Springer A++ Viewer
    PublisherInfo PublisherName : BioMed Central PublisherLocation : London PublisherImprintName : BioMed Central Ira Herskowitz dies ArticleInfo ArticleID : 4766 ArticleDOI : 10.1186/gb-spotlight-20030506-01 ArticleCitationID : spotlight-20030506-01 ArticleSequenceNumber : 118 ArticleCategory : Research news ArticleFirstPage : 1 ArticleLastPage : 4 RegistrationDate : 2003–5–6 ArticleHistory : OnlineDate : 2003–5–6 ArticleCopyright : BioMed Central Ltd2003 ArticleGrants : ArticleContext : 130594411 Brendan Maher Email: [email protected] Ira Herskowitz, professor of genetics at the University of California, San Francisco (UCSF), died at home on April 28 of pancreatic cancer. He was 56. Remembered for clarity of mind, exceptional science, enthusiastic teaching, and a love of music, his death sent a shock throughout the yeast community this week, where Herskowitz made many great contributions and friends. "It's a great loss to us all," said Paul Nurse, Nobel Laureate and new president of The Rockefeller University. "Ira Herskowitz had a huge influence on the yeast field and on me personally." Laureate Leland Hartwell, president of Fred Hutchinson Cancer Research Center told us in an e-mail, "I'll bet there are few scientists in genetics, molecular and cell biology who were not personally affected by him." Born in Brooklyn, NY, Herskowitz moved with his family about the country as father Irwin, a Drosophilageneticist, took various academic positions. Herskowitz received a bachelor's degree in science from the California Institute of Technology in 1967 and a PhD in microbiology from the Massachusetts Institute of Technology (MIT) in 1971. After a short postdoctoral stint there, he assumed an assistant professor position at the University of Oregon. Moving in 1982 to UCSF, he immediately revamped the genetics program there, chaired the department of biochemistry and biophysics from 1990 to 1995, and co-directed the program in human genetics from 1997.
    [Show full text]
  • The Polyp and the Medusa Life on the Move
    The Polyp and the Medusa Life on the Move Millions of years ago, unlikely pioneers sparked a revolution. Cnidarians set animal life in motion. So much of what we take for granted today began with Cnidarians. FROM SHAPE OF LIFE The Polyp and the Medusa Life on the Move Take a moment to follow these instructions: Raise your right hand in front of your eyes. Make a fist. Make the peace sign with your first and second fingers. Make a fist again. Open your hand. Read the next paragraph. What you just did was exhibit a trait we associate with all animals, a trait called, quite simply, movement. And not only did you just move your hand, but you moved it after passing the idea of movement through your brain and nerve cells to command the muscles in your hand to obey. To do this, your body needs muscles to move and nerves to transmit and coordinate movement, whether voluntary or involuntary. The bit of business involved in making fists and peace signs is pretty complex behavior, but it pales by comparison with the suites of thought and movement associated with throwing a curve ball, walking, swimming, dancing, breathing, landing an airplane, running down prey, or fleeing a predator. But whether by thought or instinct, you and all animals except sponges have the ability to move and to carry out complex sequences of movement called behavior. In fact, movement is such a basic part of being an animal that we tend to define animalness as having the ability to move and behave.
    [Show full text]
  • Course Outline for Biology Department Adeyemi College of Education
    COURSE CODE: BIO 111 COURSE TITLE: Basic Principles of Biology COURSE OUTLINE Definition, brief history and importance of science Scientific method:- Identifying and defining problem. Raising question, formulating Hypotheses. Designing experiments to test hypothesis, collecting data, analyzing data, drawing interference and conclusion. Science processes/intellectual skills: (a) Basic processes: observation, Classification, measurement etc (b) Integrated processes: Science of Biology and its subdivisions: Botany, Zoology, Biochemistry, Microbiology, Ecology, Entomology, Genetics, etc. The Relevance of Biology to man: Application in conservation, agriculture, Public Health, Medical Sciences etc Relation of Biology to other science subjects Principles of classification Brief history of classification nomenclature and systematic The 5 kingdom system of classification Living and non-living things: General characteristics of living things. Differences between plants and animals. COURSE OUTLINE FOR BIOLOGY DEPARTMENT ADEYEMI COLLEGE OF EDUCATION COURSE CODE: BIO 112 COURSE TITLE: Cell Biology COURSE OUTLINE (a) A brief history of the concept of cell and cell theory. The structure of a generalized plant cell and generalized animal cell, and their comparison Protoplasm and its properties. Cytoplasmic Organelles: Definition and functions of nucleus, endoplasmic reticulum, cell membrane, mitochondria, ribosomes, Golgi, complex, plastids, lysosomes and other cell organelles. (b) Chemical constituents of cell - salts, carbohydrates, proteins, fats
    [Show full text]
  • Hydra Effects in Stable Communities and Their Implications for System Dynamics
    Ecology, 97(5), 2016, pp. 1135–1145 © 2016 by the Ecological Society of America Hydra effects in stable communities and their implications for system dynamics MICHAEL H. CORTEZ,1,3 AND PETER A. ABRAMS2 1Department of Mathematics and Statistics, Utah State University, Logan, Utah 84322, USA 2Department of Ecology and Evolutionary Biology, University of Toronto, 25 Harbord St., Toronto, ON M5S 3G5, Canada Abstract. A hydra effect occurs when the mean density of a species increases in response to greater mortality. We show that, in a stable multispecies system, a species exhibits a hydra effect only if maintaining that species at its equilibrium density destabilizes the system. The stability of the original system is due to the responses of the hydra-effect species to changes in the other species’ densities. If that dynamical feedback is removed by fixing the density of the hydra-effect species, large changes in the community make-up (including the possibility of species extinction) can occur. This general result has several implications: (1) Hydra effects occur in a much wider variety of species and interaction webs than has previously been described, and may occur for multiple species, even in small webs; (2) conditions for hydra effects caused by predators (or diseases) often differ from those caused by other mortality factors; (3) introducing a specialist or a switching predator of a hydra-effect species often causes large changes in the community, which frequently involve extinction of other species; (4) harvest policies that attempt to maintain a constant density of a hydra-effect species may be difficult to implement, and, if successful, are likely to cause large changes in the densities of other species; and (5) trophic cascades and other indirect effects caused by predators of hydra-effect species can exhibit amplification of effects or unexpected directions of change.
    [Show full text]
  • Curriculum Vitae Jasper Rine Address: Work Home Department of Molecular and Cellular Biology 400 Western Drive Division of Genet
    Curriculum Vitae Jasper Rine Address: Work Home Department of Molecular and Cellular Biology 400 Western Drive Division of Genetics Richmond, California 94801 California Institute for Quantitative Biosciences 374A Stanley Hall 510-232-4293 University of California Birth Date: October 16, 1953 Berkeley, CA 94720-3202 Tel: 510-642-7047 Fax: 510-666-2768 Higher Education Post-doctoral Fellow 1980-1982 Stanford University School of Medicine Advisor: Ronald W. Davis University of Oregon, Ph.D. 1975-1979 Molecular Genetics Advisor: Ira Herskowitz State University of New York at Albany 1971-1975 B.S. Biological Sciences, Magna Cum Laude Professional Experience Professor of Genetics Division of Genetics, Genomics and Development Department of Molecular and Cell Biology University of California, Berkeley 1990-Present Director, Human Genome Center Lawrence Berkeley Labs Berkeley, California 1991-1994 Associate Professor of Genetics Division of Genetics Department of Molecular and Cell Biology University of California, Berkeley 1989-1990 Assistant Professor of Biochemistry Department of Biochemistry University of California, Berkeley 1982-1988 Honors/Awards N.I.H. Postdoctoral Fellowship 1980-1982 The Camille and Henry Dreyfus Teacher Scholar Award 1986 Miller Research Professor, UC Berkeley 1993 Philips Distinguished Lecturer, Haverford College 1993 American Academy of Microbiology – Fellow Election 1993 Streisinger Lecturer, University of Oregon 1993 UCB Distinguished Teaching Award 1997 Richard & Rhoda Goldman Distinguished Professor of Biology
    [Show full text]
  • How to Use Hydra As a Model System to Teach Biology in the Classroom PATRICIA BOSSERT1 and BRIGITTE GALLIOT*,2
    Int. J. Dev. Biol. 56: 637-652 (2012) doi: 10.1387/ijdb.123523pb www.intjdevbiol.com How to use Hydra as a model system to teach biology in the classroom PATRICIA BOSSERT1 and BRIGITTE GALLIOT*,2 1University of Stony Brook, New York, USA and 2 Department of Genetics and Evolution, University of Geneva, Switzerland ABSTRACT As scientists it is our duty to fight against obscurantism and loss of rational thinking if we want politicians and citizens to freely make the most intelligent choices for the future gen- erations. With that aim, the scientific education and training of young students is an obvious and urgent necessity. We claim here that Hydra provides a highly versatile but cheap model organism to study biology at any age. Teachers of biology have the unenviable task of motivating young people, who with many other motivations that are quite valid, nevertheless must be guided along a path congruent with a ‘syllabus’ or a ‘curriculum’. The biology of Hydra spans the history of biology as an experimental science from Trembley’s first manipulations designed to determine if the green polyp he found was plant or animal to the dissection of the molecular cascades underpinning, regenera- tion, wound healing, stemness, aging and cancer. It is described here in terms designed to elicit its wider use in classrooms. Simple lessons are outlined in sufficient detail for beginners to enter the world of ‘Hydra biology’. Protocols start with the simplest observations to experiments that have been pretested with students in the USA and in Europe. The lessons are practical and can be used to bring ‘life’, but also rational thinking into the study of life for the teachers of students from elementary school through early university.
    [Show full text]
  • OREGON ESTUARINE INVERTEBRATES an Illustrated Guide to the Common and Important Invertebrate Animals
    OREGON ESTUARINE INVERTEBRATES An Illustrated Guide to the Common and Important Invertebrate Animals By Paul Rudy, Jr. Lynn Hay Rudy Oregon Institute of Marine Biology University of Oregon Charleston, Oregon 97420 Contract No. 79-111 Project Officer Jay F. Watson U.S. Fish and Wildlife Service 500 N.E. Multnomah Street Portland, Oregon 97232 Performed for National Coastal Ecosystems Team Office of Biological Services Fish and Wildlife Service U.S. Department of Interior Washington, D.C. 20240 Table of Contents Introduction CNIDARIA Hydrozoa Aequorea aequorea ................................................................ 6 Obelia longissima .................................................................. 8 Polyorchis penicillatus 10 Tubularia crocea ................................................................. 12 Anthozoa Anthopleura artemisia ................................. 14 Anthopleura elegantissima .................................................. 16 Haliplanella luciae .................................................................. 18 Nematostella vectensis ......................................................... 20 Metridium senile .................................................................... 22 NEMERTEA Amphiporus imparispinosus ................................................ 24 Carinoma mutabilis ................................................................ 26 Cerebratulus californiensis .................................................. 28 Lineus ruber .........................................................................
    [Show full text]
  • Freshwater Jellyfish
    Freshwater Jellyfish Craspedacusta sowerbyi www.seagrant.psu.edu Species at a Glance While similar in appearance to its saltwater relative, the freshwater jellyfish is actually considered a member of the hydra family, and not a “true” jellyfish. It is widespread around the world and has been in the United States since the early 1900’s. Even though this jellyfish uses stinging cells to capture prey, these stingers are too small to penetrate Map courtesy of United States human skin and are not considered a threat to people. Geological Survey. FRESHWATER JELLYFISH Species Description Craspedacusta sowerbyi The freshwater jellyfish exists in two main forms throughout its life. In the juvenile phase, it is a small, 1 mm long gelatinous polyp that lacks tentacles. In this phase the jellyfish attaches to hard surfaces and forms colonies. The freshwater jellyfish is most easily identified in its adult phase, as it has many of the characteristics of a true jellyfish such as a small, bell-shaped transparent body that is 5-25 mm in diameter. A whorl of string-like tentacles surround the circular edge of the body in sets of three to seven. The tentacles contain hundreds of specialized stinging cells that aid in capturing prey and protecting against predators. Native HUCs HUC 8 Level Record Native & Introduced Ranges HUC 6 Level Record Non-specific State Record Originally from the Yangtze River valley in China, the fresh- Map created on 8/5/2017. water jellyfish can now be found on all continents worldwide. It was first reported in the United States in the early 1900’s, presumably introduced with the transport of stocked fish and aquatic plants.
    [Show full text]
  • From Controlling Elements to Transposons: Barbara Mcclintock and the Nobel Prize Nathaniel C
    454 Forum TRENDS in Biochemical Sciences Vol.26 No.7 July 2001 Historical Perspective From controlling elements to transposons: Barbara McClintock and the Nobel Prize Nathaniel C. Comfort Why did it take so long for Barbara correspondence. From these and other to prevent her controlling elements from McClintock (Fig. 1) to win the Nobel Prize? materials, we can reconstruct the events moving because their effects were difficult In the mid-1940s, McClintock discovered leading up to the 1983 prize*. to study when they jumped around. She genetic transposition in maize. She What today are known as transposable never had any inclination to pursue the published her results over several years elements, McClintock called ‘controlling biochemistry of transposition. and, in 1951, gave a famous presentation elements’. During the years 1945–1946, at Current understanding of how gene at the Cold Spring Harbor Symposium, the Carnegie Dept of Genetics, Cold activity is regulated, of course, springs yet it took until 1983 for her to win a Nobel Spring Harbor, McClintock discovered a from the operon, François Jacob and Prize. The delay is widely attributed to a pair of genetic loci in maize that seemed to Jacques Monod’s 1960 model of a block of combination of gender bias and gendered trigger spontaneous and reversible structural genes under the control of an science. McClintock’s results were not mutations in what had been ordinary, adjacent set of regulatory genes (Fig. 2). accepted, the story goes, because women stable alleles. In the term of the day, they Though subsequent studies revealed in science are marginalized, because the made stable alleles into ‘mutable’ ones.
    [Show full text]
  • H Size Determination in Hydra: the Roles of Growth and Budding
    t /. Embryol. exp. Morph. Vol. 30, l,pp. 1-19, 1973 Printed in Great Britain h Size determination in Hydra: The roles K of growth and budding By JOHN W. BISBEE1 ^ From the Department of Biology, University of Pittsburgh r P SUMMARY \~ Hydra pseudoligactis cultured at 9 °C for 3-4 weeks are one-and-a-half times larger than ^ those cultured at 18 °C. The size of Hydra is correlated with the numbers of epithelio- muscular and digestive cells in the distal portion of the animal and with the diameters of the k- epithelio-muscular cells in the peduncle. [ Counts of mitotic figures and tritiated-thymidine-labeled nuclei and determinations of T increase in mass of Hydra populations suggest that the difference caused by these tempera- y. tures does not affect mitosis. At 9 °C buds are initiated at a lower rate and take longer to develop than at 18 °C. The surface-areas of buds raised at the two temperatures are similar. T Because Hydra raised at the two temperatures have similar growth dynamics, the differences u in sizes of the animals cannot be due to growth rate. The observed effect of temperature on bud initiation and development is probably relevant to the increased size of animals raised at 9 "C, since these larger animals may be accumulating more cells while losing fewer to buds. INTRODUCTION The shape and size of Hydra seems to be a consequence of several dynamic processes. Growth, budding, cell sloughing, cell migration, and mesogleal metabolism have possible roles in Hydra morphogenesis (Burnett, 1961, 1966; Burnett & Hausman, 1969; Brien & Reniers-Decoen, 1949; Campbell, 1965, 1967 a, b, c, 1968; Shostak, Patel & Burnett, 1965; Shostak & Globus, 1966; Shostak, 1968).
    [Show full text]
  • 2020 Online Session Descriptions
    Thursday, April 16 2:00 pm - 6:00 pm Mammalian Trainee Symposium Session Chairs: Fernando Pardo-Manuel de Villena, UNC Chapel Hill Linda Siracusa, Hackensack Meridian School of Medicine at Seton Hall University 538A 2:00 pm No more paywalls: cost-benefit analysis across scRNA-seq platforms reveals biological insight is reproducible at low sequencing depths. Kathryn McClelland, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK/NIH) 882C 2:15 pm Control of target gene specificity in Wnt signaling by transcription factor interactions. Aravindabharathi Ramakrishnan, University of Michigan, Ann Arbor 2217C 2:30 pm Evolutionary genomics of centromeric satellites in House Mice (Mus). Uma Arora, The Jackson Laboratory 2:45 pm Reference quality mouse genomes reveal complete strain-specific haplotypes and novel functional loci. Mohab Helmy, EMBL-EBI 887B 3:00 pm Divergence in KRAB zinc finger proteins is associated with pluripotency spectrum in mouse embryonic stem cells. Candice Byers Jackson Laboratory 531C 3:15 pm Replicability and reproducibility of genetic analysis between different studies using identical Collaborative Cross inbred mice. UNC CHAPEL HILL 3:30 pm Proteomics reveals the role of translational regulation in ES cells. Selcan Aydin, The Jackson Laboratory for Mouse Genetics 563B 3:45 pm Super-Mendelian inheritance mediated by CRISPR–Cas9 in the female mouse germline. Hannah Grunwald, University of California San Diego 2103C 4:00 pm Gene Editing ELANE in Human Hematopoietic Stem and Progenitor Cells Reveals Variant Pathogenicity and Therapeutic Strategies for Severe Congenital Neutropenia. Shuquan Rao, Boston Childrens Hospital 4:15 pm Phase separation of YAP reorganizes genome topology for long-term YAP target gene expression.
    [Show full text]
  • Hydra Magnipapillata
    A Proposal to Construct a BAC Library from Hydra magnipapillata Submitted by Robert E. Steele and Hans R. Bode University of California, Irvine The importance of the organism to biomedical or biological research. Hydra has been the subject of experimental studies for over 200 years (Trembley, 1744). Its attractive features include (1) its ease of culture in the laboratory, (2) its simple structure, (3) a small number of cell types, (4) three cell lineages, each of which is a stem cell lineage, and (5) an extensive capacity for regeneration. Because of the tissue dynamics of an adult Hydra, the developmental processes governing pattern formation, morphogenesis, cell division, and cell differentiation are continuously active. Given its considerable capacity for regeneration as well as its being amenable to a variety of manipulations at the tissue and cell levels, most of the work up to the mid-1980s was focused on aspects of the developmental biology of Hydra. Primarily, these efforts involved gaining an understanding of formation and patterning of the single axis of the animal (Bode and Bode, 1984) as well as understanding the nature and control of cell division and differentiation of the stem cell lineages (Bode, 1996). With these aspects fairly well established, the emphasis shifted in the late 1980s to gaining an understanding of the molecular mechanisms underlying the elucidated developmental processes. Efforts were focused on looking for orthologues of genes affecting patterning and stem cell processes in bilaterians, which led to the isolation and characterization of >100 such genes. The variety of cell and tissue manipulations that had been developed proved, and are proving, valuable in sorting out the developmental roles of these genes with some precision.
    [Show full text]