Supporting Information for Proteomics DOI 10.1002/pmic.200400896

Odette Prat, Frdric Berenguer, Vronique Malard, Emmanuelle Tavan, Nicole Sage, Grard Steinmetz and Eric Quemeneur

Transcriptomic and proteomic responses of renal HEK293 cells to uranium toxicity

ª 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.de Table 1 : Differentially expressed in HEK293 cells treated with uranium at CI50 , CI30 and CI20. ID GENE DESCRIPTION CI50 CI30 CI20

AANAT arylalkylamine N-acetyltransferase 1.66

AASDHPPT aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase -1.73

ABCC8 ATP-binding cassette, sub-family C (CFTR/MRP), member 8 2.96 2.9

ABCF2 ATP-binding cassette, sub-family F (GCN20), member 2 1.86

ACAT2 acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase) -2.47

ACTB actin, beta -2.12

ACTR2 ARP2 actin-related 2 homolog (yeast) -1.94

ADAR adenosine deaminase, RNA-specific 1.87 1.92

ADNP activity-dependent neuroprotector -1.03

ADPRTL1 ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1 1.48 2.31 2.1

AKAP1 A kinase (PRKA) anchor protein 1 1.59

AKR1C3 aldo-keto reductase family 1, member C3 -1.37

(3-alpha hydroxysteroid dehydrogenase, type II)

ALS2CR3 amyotrophic lateral sclerosis 2 (juvenile) region, candidate 3 -1.21

APBB1 amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) 4.41

APC adenomatosis polyposis coli -1.66

APP amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) -1.32

APPBP1 amyloid beta precursor protein binding protein 1, 59kDa -1.55

APPL adaptor protein containing pH domain, PTB domain and zipper motif 1.44

ARHA ras homolog gene family, member A -1.64

ATP6V1A1 ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A, isoform 1 1.97

ATP9B ATPase, Class II, type 9B 2.56 1.41

B2M beta-2-microglobulin 1.57 -2.55

BA108L7.2 similar to rat tricarboxylate carrier-like protein 2.27

BAALC brain and acute leukemia, cytoplasmic -1.39

BACE beta-site APP-cleaving enzyme -1.29

BBP beta-amyloid binding protein precursor 1.77 1.73

BGN biglycan 1.68 1.88

BIRC1 baculoviral IAP repeat-containing 1 2.36 2.13

BTF3 basic factor 3 -2.39

C15orf15 chromosome 15 open reading frame 15 -1.73

C20orf12 chromosome 20 open reading frame 12 2.6 2.52 2.14

C20orf155 chromosome 20 open reading frame 155 1.79

C20orf30 chromosome 20 open reading frame 30 2.11

C2orf9 open reading frame 9 1.86 1.82 1.9

CALM2 calmodulin 2 (phosphorylase kinase, delta) -2.83 Table 1 (continued)

CAMK2D calcium/calmodulin-dependent protein kinase (CaM kinase) II delta 1.13

CANX calnexin -2.18

CBARA1 calcium binding atopy-related autoantigen 1 3.55

CBFA2T2 core-binding factor, runt domain, alpha subunit 2 ; translocated to, 2 1.88 1.77

CGB chorionic gonadotropin, beta polypeptide 2.18

CLSTN3 calsyntenin 3 -1.47

CNIL cornichon-like -2.11

COG7 component of oligomeric golgi complex 7 1.7

COP9 COP9 homolog -1.68

COPS3 COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis) -1.39

CRMP5 collapsin response mediator protein-5 ; CRMP3-associated molecule 2.04 1.94 1.96

CRYBB2 core-binding factor, runt domain, alpha subunit 2 ; translocated to, 2 1.47

CTCF CCCTC-binding factor (zinc finger protein) -2.39

CTRL chymotrypsin-like 3.3

CUL2 cullin 2 1.32

D1S155E NRAS-related gene -2.11

dA141H5.1 Likely ortholog of mouse neuralin 1 (NRLN1) 1.53 1.39 1.59

DDEF2 development and differentiation enhancing factor 2 -1.25

DGKA diacylglycerol kinase, alpha 80kDa 1.95

DKFZp434N0650 hypothetical protein DKFZp434N0650 -2 -1.61

DKFZP564A2416 DKFZP564A2416 protein -1.18

DKFZp761N1114 hypothetical protein DKFZp761N1114 2.49 2.91

DNAJA2 DnaJ (Hsp40) homolog, subfamily A, member 2 -1.47

DNAJC9 DnaJ (Hsp40) homolog, subfamily C, member 9 2

DSCR2 Down syndrome critical region gene 2 -1.35

DTNA dystrobrevin, alpha 1.22 2.04 2.03

DTYMK deoxythymidylate kinase (thymidylate kinase) 1.58 1.98

E46L like mouse brain protein E46 -2.11

EEF1A1 eukaryotic translation elongation factor 1 alpha 1 -1.58

EEF1G eukaryotic translation elongation factor 1 gamma -2.83 -1.76

EPS15 epidermal growth factor receptor pathway substrate 15 -1.21

ERAP140 endoplasmic reticulum associated protein 140 kDa 2.42

ERBB3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) 1.63

EVI5 ecotropic viral integration site 5 2.36

FAM3C family with sequence similarity 3, member C -1.29

FBXL3A F-box and leucine-rich repeat protein 3A -1.73

FLJ10326 hypothetical protein FLJ10326 -1.15 Table 1 (continued)

FLJ10803 hypothetical protein FLJ10803 3.29

FLJ10849 hypothetical protein FLJ10849 1.74

FLJ11040 hypothetical protein FLJ11040 -1.55

FLJ12270 hypothetical protein FLJ12270 1.57

FLJ12448 hypothetical protein FLJ12448 2.15

FLJ12565 hypothetical protein FLJ12565 -2.47

FLJ12584 hypothetical protein FLJ12584 1.7 1.94 2.05

FLJ12666 hypothetical protein FLJ12666 2.32 2.25 1.99

FLJ12747/KIAA1133 hypothetical protein FLJ12747/novel C3HC4 type Zinc finger (ring finger) 1.89

FLJ12888 hypothetical protein FLJ12888 1.13

FLJ13110 hypothetical protein FLJ13110 -1.11

FLJ20783 hypothetical protein FLJ20783 2.36

FLJ20886/GRB2 hypothetical protein FLJ20886/growth factor receptor-bound protein 2 1.52

FLJ30594 hypothetical protein FLJ30594 2.06

FLJ30596 hypothetical protein FLJ30596 2.51 1.99 2.62

FLJ31657 hypothetical protein FLJ31657 -1.43

FUT8 fucosyltransferase 8 (alpha (1,6) fucosyltransferase) -1.51

G3BP Ras-GTPase-activating protein SH3-domain-binding protein -1.08

GLO1 glyoxalase I -1.34

GLUL glutamate-ammonia ligase (glutamine synthase) -2

GPX1 glutathione peroxidase 1 2.21

GSPT1 G1 to S phase transition 1 2.67 1.85 1.66

GTF2H2 general transcription factor IIH, polypeptide 2, 44kDa 2.16

HADHSC L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain 2.26

HBP1 HMG-box containing protein 1 2.9 2.53

HCS cytochrome c -1.51

HLA-E major histocompatibility complex, class I, E 1.33

HMGB1 high-mobility group box 1 -1.78

HMP19 HMP19 protein 1.22 1.48

HNRPC heterogeneous nuclear ribonucleoprotein C (C1/C2) -1.51 -1.64

HSPC228 hypothetical protein HSPC228 -1.15

HSPCB heat shock 90kDa protein 1, beta -2

ID2 inhibitor of DNA binding 2, dominant negative helix-loop-helix protein -2.06 -1.88

IFRD2 interferon-related developmental regulator 2 1.13

IK IK cytokine, down-regulator of HLA II -1.35

INSIG1 insulin induced gene 1 -1.55

IQGAP2 IQ motif containing GTPase activating protein 2 2.07 Table 1 (continued)

KATII L-kynurenine/alpha-aminoadipate aminotransferase -2.32

KIAA0171 Enthoprotin protein -1.28

KIAA0275 KIAA0275 protein -1.64

KIAA0453 KIAA0453 protein -2.55

KIAA0472 KIAA0472 protein 1.86

KIAA0532 KIAA0532 protein 1.62

KIAA0594 KIAA0594 protein 3.04 3.09

KIAA0876 KIAA0876 protein 3.26 2.03 1.73

KIAA0923 KIAA0923 protein -1.58

KIAA1026 KIAA1026 protein 1.64

KIAA1086 KIAA1086 protein 1.39

KIAA1143 KIAA1143 protein 3.05 2.42 1.82

KIAA1155 KIAA1155 protein 1.6

KIAA1354 KIAA1354 protein 1.28

KIAA1467 KIAA1467 protein 2.43 2.07

KIAA1724 KIAA1724 protein 2.05 1.51

KIAA1735 KIAA1735 protein 1.83

KIAA1841 KIAA1841 protein 2.49 2.18

KIAA1948 KIAA1948 protein 1.58

KIF1B kinesin family member 1B 1.94 2.33 2.24

KIF3B kinesin family member 3B 1.63

KIF3C kinesin family member 3C -2.64

KPNA3 karyopherin alpha 3 (importin alpha 4) -1.58

KPNB2 karyopherin (importin) beta 2 2.23 1.66

LAD1 ladinin 1 1.19

LAMP2 lysosomal-associated membrane protein 2 2.83

LDHA lactate dehydrogenase A -2.94

LOC121512/KIAA1615 KIAA1615 protein 2.24

LOC123016 hypothetical protein BC001563 2.14 1.97

LOC51030 CGI-148 protein -1.83

LOC56990 non-kinase Cdc42 effector protein SPEC2 3.21

LOC57038 similar to arginyl-tRNA synthetase 2.45

LOC57146 hypothetical protein from clone 24796 1.51 1.84

LPIN1 lipin 1 1.13

M6PR mannose-6-phosphate receptor (cation dependent) -1.28

MARCKS myristoylated -rich protein kinase C substrate -2.18

MAT2B methionine adenosyltransferase II, beta -1.47 Table 1 (continued)

MDH2 malate dehydrogenase 2, NAD (mitochondrial) -1.23

METAP2 methionyl aminopeptidase 2 -1.88

MGC10485 hypothetical protein MGC10485 1.34

MGC10924 similar to arginyl-tRNA synthetase -1.47

MGC11316/KIAA1821 rab11-family interacting protein 4(RAB11-FIP4) 1.78

MGC21675 hypothetical protein MGC21675 1.21 2.24 2.09

MGC34032 hypothetical protein MGC34032 2.25 2.02

MGC4707 hypothetical protein MGC4707 1.9

MGC4840 LONP peroxisomal Lon protease 2.82 2.16 2.2

MLLT10 myeloid/lymphoid or mixed-lineage leukemia 1.64

(trithorax homolog, Drosophila) ; translocated to, 10

MSH2 mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) -1.68

My015 My015 3.14 2.26 2.01

NAP1L1 nucleosome assembly protein 1-like 1 -1.94

NARS asparaginyl-tRNA synthetase -2.32

NDRG2 NDRG family member 2 1.34

NDRG4 NDRG family member 4 2.35 1.49 2.09

NEFL neurofilament, light polypeptide 68kDa -1.43

NEIL2 nei like 2 (E. coli) 1.47

NICE-3 NICE-3 protein -1.78

NOTCH2 Notch homolog 2 (Drosophila) -1.83

NPEPL1 aminopeptidase-like 1 1.93 1.65

NRF1 nuclear respiratory factor 1 2.06

NT5E 5’-nucleotidase, ecto (CD73) -1.94

OAT ornithine aminotransferase (gyrate atrophy) -2

P23 P23 protein -1.35

PAI-RBP1 PAI-1 mRNA-binding protein -1.78

PBEF P23 protein -2

PBX3 pre-B-cell leukemia transcription factor 3 2.56

PCCX2 protein containing CXXC domain 2 1.74

PDCD6IP programmed cell death 6 interacting protein -1.28

PDCD7 programmed cell death 7 2.45 1.95

PDE1A phosphodiesterase 1A, calmodulin-dependent 1.62

PFKFB4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 1.31

PGD phosphogluconate dehydrogenase -1.78

PI4KII phosphatidylinositol 4-kinase type II 1.61

PIK3R1 phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) 1.2 Table 1 (continued)

PIP5K2B phosphatidylinositol-4-phosphate 5-kinase, type II, beta 1.51

PLU-1 putative DNA/chromatin binding motif -1.08

PPIA peptidylprolyl isomerase A (cyclophilin A) -1.83

PPP2CA protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform -1.47

PRDX2 peroxiredoxin 2 -1.51

PRPF4B PRP4 pre-mRNA processing factor 4 homolog B (yeast) -1.32

PSMA4 proteasome (prosome, macropain) subunit, alpha type, 4 -2.11

PTK2 PTK2 protein tyrosine kinase 2 -1.94

PTMA prothymosin, alpha (gene sequence 28) -2

PUM1 pumilio homolog 1 (Drosophila) -1.64

RAB2 RAB2, member RAS oncogene family -1.51

RAB7 RAB7, member RAS oncogene family -1.39

RABGGTB Rab geranylgeranyltransferase, beta subunit -1.02

RAC1 ras-related C3 botulinum toxin substrate 1 -2.05

(rho family, small GTP binding protein Rac1)

RAN RAN, member RAS oncogene family -3.32

RANBP7 RAN binding protein 7 -1.68

RBBP7 retinoblastoma binding protein 7 -1.32

RBM3 RNA binding motif protein 3 2.62 1.87 1.31

RBM8A RNA binding motif protein 8A -1.35

RDX radixin -1.39

RGL RalGDS-like gene -1.11

RNB6 RNB6 -1.68

RNF10 ring finger protein 10 -1.25

RNGTT RNA guanylyltransferase and 5'-phosphatase -2.11

RPA1 replication protein A1, 70kDa -1.55

RPL15 ribosomal protein L15 -2.83

RPL22 ribosomal protein L22 -1.66

RPL24 ribosomal protein L24 -2.18

SBA2 CS box-containing WD protein -1.43

SCAMP1 secretory carrier membrane protein 1 1.38

SCN2B sodium channel, voltage-gated, type II, beta polypeptide 2.14 1.72

SCP2 sterol carrier protein 2 -1.88

SDC3 syndecan 3 (N-syndecan) 2.4 -1.76

SDCBP syndecan binding protein (syntenin) -2.11

SDNSF neural stem cell derived neuronal survival protein 2

SEC24B SEC24 related gene family, member B (S. cerevisiae) -3.32 Table 1 (continued)

SEPX1 selenoprotein X, 1 -1.83

SLC17A2 solute carrier family 17 (sodium phosphate), member 2 1.54

SLC25A6 solute carrier family 25 (mitochondrial carrier ; adenine nucleotide translocator), -2.55 -2.11

member 6

SLC2A3 solute carrier family 2 (facilitated glucose transporter), member 3 1.65 1.53

SMAP1 stromal membrane-associated protein 2.12

SMARCC1 SWI/SNF complex -1.51

SNAP91 synaptosomal-associated protein, 91kDa homolog (mouse) -1.21

SNX6 sorting nexin 6 -1.35

SPP2 secreted phosphoprotein 2, 24kDa 2.93

SRRM1 serine/arginine repetitive matrix 1 -1.47

STK36 serine/threonine kinase 36 (fused homolog, Drosophila) 1.88

STMN1 stathmin 1/oncoprotein 18 -1.78

STMN2 stathmin-like 2 -1.65

SUI1 putative translation initiation factor -2.32

SYBL1 SWI/SNF complex -1.53

TALDO1 transaldolase 1 -2.06

TDG thymine-DNA glycosylase -1.39

TFAM transcription factor A, mitochondrial -1.47

TGIF2 TGFB-induced factor 2 (TALE family homeobox) 1.15

TGOLN2 trans-golgi network protein 2 2.26

TLK1 tousled-like kinase 1 -1.47

TLOC1 translocation protein 1 -1.43

TNFRSF1B tumor necrosis factor receptor superfamily, member 1B 1.95

TNFRSF9 tumor necrosis factor receptor superfamily, member 9 1.36

TNP1 transition protein 1 (during histone to protamine replacement) 1.19

TOMM20 translocase of outer mitochondrial membrane 20 (yeast) homolog -1.64

TRIM28 tripartite motif-containing 28 -2.18

TUBA1 tubulin, alpha 1 (testis specific) -1.11

TUBA3 tubulin, alpha 3 -2.83

UBCE7IP4 likely ortholog of mouse ubiquitin conjugating enzyme 7 interacting protein 4 2.53 2.46

UBE2D2 ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) -1.39

ULK2 unc-51-like kinase 2 (C. elegans) -1.55

UNG uracil-DNA glycosylase -1.11

VDAC2 voltage-dependent anion channel 2 -1.94

VSNL1 visinin-like 1 1.63

WASL Wiskott-Aldrich syndrome-like -1.73 Table 1 (continued)

WFDC2 WAP four-disulfide core domain 2 1.73 2.01

WIT-1 Wilms tumor associated protein 2.12

WNT7B wingless-type MMTV integration site family, member 7B 2.17

XAP135 PHD zinc finger protein XAP135 2.73

YWHAQ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, -1.83

theta polypeptide

Values are mean of Log2 R of dyeswaps replicates. Values of Log2 R higher and lower than 1 and –1 stand for more than two fold induced or repressed mRNAs, respectively. Modulated genes were selected when differentially expressed in at least two time-points for a given concentration. The most extreme value is indicated in the table. Footnote Table 1. Overlapping gene response at 3 concentration-levels of U are in bold. Table 2. List of 65 of HEK293 identified on 2-DE reference map by MALDI-TOF analysis.

SWISS-PROT Mascot SEQUENCE MATCHING NAME PROTEIN IDENTITY ppm Mr pI Accession number score COVERAGE % PEPTIDES

O75347 TBCA tubulin specific chaperone A 175 74/52 27 7 12724 5.25

hypoxanthine guanine phosphorybosyl P00492 HPRT 100 68/52 28 5 24448 6.24 transférase

P00918 CAH2 Carbonic Anhydrase II 40 64/52 13 5 29154 6.86

P00938 TPIS Triosephosphataseisomerase (TPIS) 45 73/52 32 7 26807 6.51

P02248 UBIQ Ubiquitin 100 55/52 51 4 8560 6.56

P02570 ACTB Actin cytoplasmic.1 (Béta actin) 30 57/52 13 5 42052 5.29

P02571 ACTG Actin cytoplasmic.2 (gamma actin) 30 57/52 13 5 42108 5.31

P02768 ALBU Serum albumin precursor 100 78/52 17 9 71317 5.92

P02787 TRFE Serotransferrine precursor 100 68/52 15 12 79280 6.81

P04075 ALFA Fructose-bisphosphate aldolase A 150 64/52 37 9 39720 8.39

P04181 OAT Ornithine aminotransferase 150 72/52 14 6 48846 6.57

glyceraldehyde-3 phosphate P04406 G3P2 125 74/52 19 11 36070 8.58 deshydrogenase

P04765 IF41 Eukayotic initiation factor 4A-I 55 54/52 18 9 46353 5.32

P05092 PPIA peptidyl prolyl cis trans isomerase A 100 86/52 43 8 17881 7.82

P05092 PPIA peptidyl prolyl cis trans isomerase A 75 78/52 39 8 17881 7.82

P05209 TBA1 Tubulin alpha-1 chain 100 63/52 21 12 50804 4.94

P05787 K2C8 Keratin ,type II cytoskeletal 8 100 116/52 20 12 53510 5.52

P06733 ENOA Alpha enolase 45 73/52 19 8 47350 6.99

P06753 TMP3 tropomyosin alpha 3 chain 50 53/52 12 5 32856 4.68

P07195 LDHB L-lactatedehydrogenase B 25 75/52 20 8 36769 5.72

P08107 HSP70 heat shock protein 70 100 98/52 16 12 70052 5.48

P08238 HS9B Heat shock protein 90 -beta 100 127/52 23 15 83423 4.97

P08865 RSP4 40s ribosomal protein SA (P40) 100 92/52 24 7 32947 4.79

P09429 HMG1 High mobility group protein 1 50 113/52 33 11 24918 5.62

P10159 IF5A eukariotic translation initiation factor 60 58/52 28 6 16918 5.08

P10809 CH60 60kDa Heat Shock Protein mitochondria 80 70/52 17 8 61187 5.7

P11021 GR78 78 kDa Glucose regulated prot precursor 120 53/52 17 9 72402 5.07

P12004 PCNA proliferating cell nuclear antigen 40 55/52 24 5 29092 4.57

P12277 KCRB creatine kinase B chain 100 82/52 20 8 42644 5.34

P13489 RINI Placental ribonuclease inhibitor 100 91/52 20 8 51635 4.71

P13796 PLSL L plastin 100 61/52 14 7 70289 5.2

P13797 PLST T-plastin 200 53/52 16 11 70904 5.52 Table 2 (continued)

P14866 ROL Heterogenous nuclear rironucleoprot. 60 68/52 21 10 60719 6.65

P15531 NDKA Nucléoside Diphosphate Kinase A 15 55/52 29 4 17309 5.83

P16949 STN 1 stathmin phosphoprotein P19 100 57/52 31 5 17171 5.77

P17987 TCPA T-complex protein 1, alpha-subunit 75 62/52 14 8 60819 5.8

P18669 PMG1 phosphoglycerate mutase, 190 58/52 15 5 28769 6.75

P20700 LAM1 lamin B1 100 105/52 21 12 66277 5.11

P22626 ROA2 Heterogenous nuclear rironucleoprot. 100 96/52 30 12 37464 8.97

P22626 ROA2 heterogeneous nuclear ribonucleoprotein 100 60/52 25 9 37464 8.97

P22626 ROA2 Heterogeneous nuclear riboprotein 60 83/52 31 11 37464 8.97

P27797 CRP55 calréticulin precursor 100 57/52 12 6 48283 4.29

P30101 PDA3 Protein disulfide isomerase A3 50 58/52 19 8 57146 5.98

P31943 ROH1 Heterogeneous nuclear precursor 20 55/52 15 7 49484 5.89

P31946 143B 14-3-3 protein beta / alpha 50 125/52 43 15 28048 4.76

P32119 PDX2 peroxiredoxin2 180 63/52 30 5 22049 5.66

P35232 PHB Prohibitin 50 99/52 26 8 29843 5.57

P35998 PRS7 26S protease regulatory subunit 7 100 53/52 12 5 48871 5.72

P38646 GR75 Stress-70 protein, mitochondrial precursor 150 69/52 16 9 73920 5.87

P40227 TCPZ T-complex protein 1, zeta-subunit 90 96/52 17 11 58444 6.23

P42655 143E 14-3-3 protein epsilon 25 52/52 21 6 29326 4.63

P43487 RANG Ran specificGTP-ase activating 100 57/52 18 5 23467 5.19

P47756 CAPB F-actin capping protein beta subnit 100 65/52 30 8 31485 5.36

P47813 IF1X eukariotic translation initiation factor 60 101/52 25 6 16433 5.07

Elongation factor TU, mitochondial P49411 EFTU 130 74/52 23 12 49852 7.26 precursor

P52565 GDIR Rho-GDP-dissociation inhibitor 50 60/52 22 6 23250 5.02

Q04760 LGUL Lactoylglutathione lyase 70 67/52 23 7 20803 5.25

Q06830 PDX1 Preoxiredoxin1 (Thyoredoxin peroxydase) 40 61/52 29 6 22324 8.27

Q13347 IF32 eukaryotic translation initiation factor 110 53/52 17 6 36878 5.38

Q14847 LAS1 Lin and SH3 domain prot 1 60 59/52 24 8 30097 6.61

Q15365 PCB1 poly (rC) binding protein 100 60/52 25 6 37526 6.66

Q16658 FSCI fascin 50 72/52 21 9 54992 6.81

Q16781 UBCN ubiquitin conjugating enzyme E2N 100 53/52 31 4 17138 6.13

Q94Q80 P2G4 proliferation associated protein 2G4 70 53/52 19 6 44101 6.13

Q9Y230 RUV2 RubB-like 2 100 123/52 31 13 51296 5,49

U-modulated proteins are in bold Table 3. Data comparison between 2-DE and DNA microarrays.

mRNA PROTEIN GENE PROTEIN NAME REPRESSION REPRESSION FUNCTIONS ID RATE a) RATE b)

HSPCB Heat shock protein 90 4 >15 Cell defense, chaperone, regulation and intracellular

signaling

HMGB1 Amphoterin (High mobility group protein 1) 2 >15 Nucleoprotein, extracellular signaling, inflammation

TUBA1 Tubulin A1 3 15 Cytoskeleton, associated to HSP90, microtubules

assembly

YWHAQ Protein 14-3-3 3 10 Intracellular signaling

ACTB Beta actin 4 3 Cytoskeleton, associated to HSP90

Footnote Table 3 : a) mRNA repression rate is the reverse of the U+/U- repression ratio b) Protein repression rate is the U-/U+ spot volumes ratio