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SHIRAI ET AL.: Aggregation of eupompe TROP. LEPID. RES., 27(2): 111-114, 2017 111

Scientific Note: Aggregation ofEpityches eupompe (: ) in southern Brazil

Leila T. Shirai, Luísa L. Mota and André V. L. Freitas

Departamento de Biologia , Instituto de Biologia, Universidade Estadual de Campinas, P.O. box 6109, CEP 13083-970 Campinas, SP, Brazil, e-mail: [email protected], [email protected], [email protected]

Abstract: An aggregation of thousands of adults of the ithomiine butterflyEpityches eupompe was observed in June-July 2017 at Intervales State Park, São Paulo State, Brazil, with another observation from an amateur naturalist at Corupá, Santa Catarina State, Brazil in September 2006. These aggregations have only been seen during winter, and they may function to provide protection from colder and/or drier climates. The phenomenon has never been previously documented. To publicize these novel observations, a short 1:32 min outreach video was produced and may be viewed via the internet at https://youtu.be/bUO4kpYS2uo, and downloaded from http://doi.org/10.5281/zenodo.1040325.

Key words: Paranapanema, Atlantic Forest, ithomiine pocket, citizen science

Resumo: Uma agregação de milhares de adultos da borboleta Epityches eupompe foi observada no Parque Estadual de Intervales, SP em Junho-Julho de 2017, além de outra ocorrência reportada por uma naturalista amadora em Corupá, SC em Setembro de 2006. A agregação foi registrada apenas no inverno, e pode estar relacionada à proteção contra locais mais frios e/ou secos. Esse fenômeno nunca foi descrito pela ciência. A fim de tornar essas observações públicas, um filme de 1:32 min para divulgação científica foi produzido junto à presente contribuição, disponível pela internet em https://youtu.be/bUO4kpYS2uo e http://doi. org/10.5281/zenodo.1040325.

Palavras chave: Paranapanema, Mata Atlântica, bolsão de ithomiine, ciência cidadã

The ithomiine Epityches eupompe (Geyer, 1832) Adults were observed hanging and perching at several (Nymphalidae: , Fig. 1) is endemic to the Neotropical heights from canopy to understory, on branches and tree Atlantic Forest, occurring in forests with different degrees of trunks of different plant , none of which were its known disturbance from south Bahia to Argentina (Brown, 1979). It solanaceaous host plants (Brown, 1987), nor presented any traces occurs from sea level (e.g., Freitas, 1996) to 2000 m altitude of herbivory (Fig. 1E, F). The stayed motionless for (e.g., Soares et al., 2011), but E. eupompe is particularly most of the time, with periods of increased activity from 11:00 common from 600 m to 1100 m (d’Almeida, 1938; AVLF, pers. to 14:00 (see movie at https://youtu.be/bUO4kpYS2uo), but obs.). From 12th-15th June and on 22nd July 2017, an aggregation only when the air temperature increased and there was no heavy of thousands of adult E. eupompe was observed at Intervales rain. When sounds of breaking or falling branches and birds in State Park, in the south of São Paulo State, in southern Brazil the near vicinity were heard, some individuals flew but soon (Figs. 1A-C; 24° 15’ 56’’S, 48° 24’ 48’’W). With 42,000 ha resumed their resting behavior, returning to the same plant or of predominantly dense montane ombrophilous forest, the park another plant on which they had previously perched. In addition, contains one of the largest continuous areas of Atlantic Forest there were no flowering plantsin situ and in surrounding areas, (Ribeiro et al., 2009), summing up to more than 120,000 ha in except for bromeliads in the upper levels of the forest, where conjunction with the contiguous Carlos Botelho and PETAR E. eupompe was not observed feeding. Local guides reported State Parks, and the Xitué Ecological Station (Nisi, 2006; that the butterflies prefer the understory when the temperature Leonel, 2010). The name “Intervales” means “between valleys” is “warm” (above 16°C), but otherwise they stay in the canopy. (Fig. 1A), in reference to the neighboring mountains that, despite A small mark-release-recapture sampling was carried out being relatively low (up to 1200 m), may reach temperatures in June 2017. A total of 30 E. eupompe adults were marked (all close to 0 °C during winter. The aggregation was observed at young, judging from the degree of wing wear, and showing no a particular site at 791 m altitude, 33 m above a river running signs of predation) with a blue permanent felt-tipped pen and from a nearby waterfall in a slope facing west (Figs. 1B, C), in individual characteristics were recorded (as in Freitas, 1996; a trail through primary forest opened 12 years ago but unused Ramos & Freitas, 1999). Each individual received a unique due to its difficulty. The aggregation was first noticed in 2013 symbol corresponding to each of the seven plant individuals by the personnel involved in trail maintenance and was seen from which they were collected, and an additional unique every year since (Ribeiro, F. A. & Conforti, T. B., pers. comm.). sequential number. The mean forewing length was 2.81 cm for Epityches eupompe (Fig. 1D) formed the aggregation from females (SD = 0.11 cm, n = 25) and 2.68 cm for males (SD = May to June as soon as the temperature decreased significantly 0.13 cm, n = 5). By exhaustive search with binoculars, a single (usually marked by frost), and dispersed from July to August marked individual was observed in situ, on a different plant (Ribeiro, F. A., Floido, J. & Amaral, B., pers. comm.). from where it was collected, and only in July 2017. 112 TROP. LEPID. RES., 27(2): 111-114, 2017 SHIRAI ET AL.: Aggregation of Epityches eupompe

Figure 1. A. General view of Intervales State Park showing the large extent of well-preserved forest (photo by Marco A. Pizo, used with his permission); B, C. close view of the area adjacent to the aggregation site (see text for details); D. male Epityches eupompe feeding on an flower; E, F. aggregation of E. eupompe on a bush in the understory and on a tree trunk near the canopy, respectively. See also the companion video at https://youtu.be/bUO4kpYS2uo. SHIRAI ET AL.: Aggregation of Epityches eupompe TROP. LEPID. RES., 27(2): 111-114, 2017 113

The aggregation was apparently not related to reproduction associated with migratory movement are common in several since only a single mating pair was observed during five days of butterfly groups, such asDanaus plexippus (Brower & Calvert, observations in June and July 2017. The near absence of mating, 1985; Davis, 2015), Nymphalis californica (Whittaker, 1953), together with the lack of males displaying their hindwing and Vanessa cardui (Knowlton, 1954; Talavera & Vila, 2017). androconial hair pencils, indicate that the aggregation does An aggregation has been reported for the ithomiine not correspond to a mating lek. Ithomiini butterflies are also hymenaea, in July 1979 in Paraguay, but the study did not relate known to form aggregations called “ithomiine pockets,” where it to an ithomiine pocket or migratory movement; it similarly several species are found in specific sites with high humidity reported a lack of evidence for oviposition and mass emergence during the dry season (Collenette & Talbot, 1928; Brown, 1972; (Strickman, 1981). Transparent Ithomiini species are not known Young, 1974; Brown & Vasconcellos-Neto, 1976; Pinheiro et to migrate large distances, although opaque, colored ithomiines al., 2008). However, Intervales does not have marked rainfall may do so, according to Brown (1977), so we suggest that the seasonality (observations in June had rain every day, as did observed E. eupompe aggregation is likely related with climatic the weeks leading up to these observations), preventing the protection from colder and/or drier weather experienced in establishment of such pockets (see Freitas, 1996). In fact, all nearby areas. If that is the case, the appropriate micro-climate other Ithomiini species observed at Intervales did not form that these thousands of butterflies are able to find year after year aggregations during the study period, nor in other years and is likely dependent on habitat preservation over the large extent seasons (LTS and AVLF pers. obs.), and only three individuals that is (still) found at Intervales (Ribeiro et al., 2009; Ribeiro & of lysimnia were seen together with the thousands Freitas, 2014). One approach to understanding this phenomenon of E. eupompe. The latter species is one of the 20 ithomiine could include a phylogeographical analysis using fast-evolving species recorded at the park, which include olena, molecular markers (microsatellites or mitochondrial genes) lenea, dero, Episcada carcinia, Episcada of individuals from this and other populations, especially hymenaea, Episcada philoclea, Episcadas striposis, southwards and at different altitudes, including a visit to adasa, ninonia, agnosia, Ithomia drymo, Corupá region to assess whether the population observed there Ithomia lichyi, , ethra, Melinaea still remains, and if it is related to the one at Intervales. If this ludovica, aquata, euryanassa, were to be pursued, recording climatic variables in situ and erruca and carlia (data from field collections by at nearby sites over months is also desirable. Further mark- LTS and AVLF, and specimens at the “Adão José Cardoso” recapture studies are not recommended because numerous Zoology Museum, Unicamp). Additionally, E. eupompe was individuals (certainly more than 30) would need to be sampled collected at other places within the park and in other months and reducing the impact of human presence is perhaps more of the year (December to April, when field expeditions were important than the result of such an experiment. Following this made by LTS and AVLF, deposited in the Zoology Museum, rationale, visits to the site should be maintained as infrequent as Unicamp under numbers ZUEC-LEP 683, 684, 832, 880, 1267, they are or become further restricted, but allowing this natural and 10253-10262), but never with this unusual high abundance. phenomenon to be seen without disturbing it is also important. It is likely that populations aggregate in other places of the park, Thus, a short video aimed at the general public was produced but we cannot point to likely factors that facilitate finding them by LTS, and is available at https://youtu.be/bUO4kpYS2uo, elsewhere, except for seeking undisturbed forest that lies at a http://doi.org/10.5281/zenodo.1040325, or https://vimeo. reasonable distance from well-used trails. Similar aggregations com/223507916/68acdd08f0. were observed by AVLF in 1992 near “Pousada Capivara” at Intervales, and an amateur lepidopterist informed us about a ACKNOWLEDGMENTS huge aggregation of E. eupompe observed on 7th September 2006, in a forest near to Corupá city, Santa Catarina State (100 We would like to thank the Parque Estadual de Intervales – 200 m altitude), about 255 km south of Intervales. It cannot be staff: Eliseu Cordeiro de Paula for providing meteorological ascertained whether the population at Intervales migrates south, data; local guides Faustino A. Ribeiro, José Floido, and over such a large distance, but we encourage the participation Benedito Amaral and the director Thiago B. Conforti for of both scientists and amateurs, in the context of citizen science informing us about the phenomenon and their outstanding (Silvertown, 2009), to report to us any relevant observations that assistance. Marco A. Pizo kindly gave us permission to use his might help us to understand this and similar natural phenomena image of Intervales. Marianne Elias, Lucas A. Kaminski, and before they disappear (Young, 1974; Davis, 2015). Keith Willmott improved the final version of the manuscript frequently surprise us with sudden or cyclic with critical suggestions. We are also grateful to Grasiela Casas demographic explosions. These population increases may and Alexandra Bächtold who told us about, and photographed, be related to reproduction (e.g., periodical 13- and 17-year the E. eupompe aggregation in Corupá, Santa Catarina; Daniel cicadas: Alexander & Moore, 1962; Cooley & Marshall, 2001), Carezzato de Almeida for editing the short video; Tamara M. abundance of host plants (e.g., cricket clouds in monoculture C. Aguiar, Artur N. Furegatti, and Michela Borges for their plantations: Drummond & Brown, 1987), and climatic assistance at the Zoology Museum, Unicamp; and Julia L. protection associated with migration (e.g., adult hibernation Pablos for helping during the June field trip. This study was in Mexico during the North American Monarch butterfly done under environmental licenses ICMBio 8585-1 (sol. migration: Brower & Calvert, 1985; and in Dominican Republic 10438) and COTEC 260108-004.611/2016. AVLF thanks by Anetia briarea: Ivie et al., 1990). Single-species aggregations CNPq (grant 303834/2015-3), RedeLep-SISBIOTA-Brasil/ 114 TROP. LEPID. RES., 27(2): 111-114, 2017 SHIRAI ET AL.: Aggregation of Epityches eupompe

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