Karyology study of Spirlin ( bipunctatus) in Zabol region

Item Type Journal Contribution

Authors Khosravanizadeh, A.; Pourkazemi, M.; Nowrozfashkhami, M.R.

Download date 26/09/2021 15:08:48

Link to Item http://hdl.handle.net/1834/10673 1392 2/ /

( Alburnoides bipunctatus )

(((333( )))3) (((222( )))2) *(*(*( 111)))1)

[email protected] 98615 538 1 41635 3464 – 23 1389 : 1389 :

: ﻛﺎرﻳﻮﺗﺎﻳﭗ، ﻣﺎﻫﻲ ﺧﻴﺎﻃﻪ، Alburnoides bipunctatus. زاﺑﻞ

. ( 1374 Alburnoides bipunctatus ( 1380 ) Abramis brama Teleostei (Bloch, 1782) ) Hypophthalmichthys molitrix Ctenopharyngodon (1381 (1381 ) idella . (1381 ) zarudnyi Barbus mursa Barbus capito ( ) ) Copoeta copoeta gracilis 1378 ) ) rufa (1384 .( 1383 Esmaeili ) Garra persica ( 1386 ( et al., 2009 . ( Esmaeili & Piravar, 2006 )Petroleuciscus pradis 23000 ) Vimba vimba persa . ( 2400 ) 10 4/

Downloaded from isfj.ir at 9:09 +0330 on Thursday November 30th 2017 [ DOI: 10.22092/ISFJ.2017.110126 ] regium ( 1389 Blicca bjoerkna (Esmaeili et al., 2010 ) ( Pourkazemi et al., 2010 ) transcaucasica Khosravanizadeh et al., ) Alburnus alburnus Nazari et )Alburnus filippii ( 2011 . ( al. , 2011 Al-Sabti, Gold et al., 1990 1381 ) .( Khosravanizadeh et al ., 2011 1991 . ) Rutilus frisii kutum 137 * ( Alburnoides bipunctatus )

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138 1392 2/ /

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( 10000 × 2() n=50 ) 1

M M

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Sm Sm

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139 ( Alburnoides bipunctatus )

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) Abramis brama ( 1374 2 n 50= (1380 ( 70 ) ( Esmaeli & Piravar, 2006 ) Petroleuciscus persidis (1386 ) Garra rufa ;Kirpichnikov, 1981 ; Khuda-Bukhsh et al. , 1986 ) ) Chalcalburnus chalcoides .( Al-sabti, 1991 ) Vimba vimba persa ( 1386 ( Cyprinidae ) Blicca bjoerkna (1389 (Pourkazemi et al. , 2010 ) transcaucasica Khosravanizadeh et al., ) Alburnus alburnus (Barbus ethiopcius , B. intermedius , B. bynni ) Nazari et )Alburnus filippii ( 2011 2n=150 2n=148 Chondrostoma 2 n 50= ( al. , 2011 Cyprinus .( Oellerman & Skelton, 1990 ) 2 n 52= ( Esmaeili et al., 2010 ) regium 2 n 98= 2 n=100 carpio ( Kalbassi et al., 2008 ) Schizothorax zarudnyi Schizothorax Schizothorax richardsonii Barbus mursa 2 n 98= Lakara et al. , ) 2 n 98= kumaonesis ) Barbus capito Chalcalburnus .( 1997 Copoeta copoeta 2 n=100 ( 1384 ( Kilic-Demürok & Unlu, 2001 ) mossulensis 2 n=150 (1384 ) gracilis 2 n 48= Ctenopharyngodon idella . .( Reddy, 1991 ) . 2 n 50= Cataudella ( 1380 ) Aristichthys nobilis Alburnus Alburnus ( 1977 ) Ctenopharyngodon idella A. heckeli A. bipunctatus alburnus ( 1381 ) . 2 n 50= ( 1381 ) Hypophthalmichthys molitrix 2 n 50= ( Alburnus bipunctatus ) ( 1384 ) Hemiculter leucisculus

Downloaded from isfj.ir at 9:09 +0330 on Thursday November 30th 2017 [ DOI: 10.22092/ISFJ.2017.110126 ] 2 n 48= ( Esmaeili et al., 2009 ) Garra persica 3(. ) ) Rutilus frisii kutum 141 ( Alburnoides bipunctatus )

A. bipunctatus 3:

88 12 38 2n=50 Cataudella et al. , 1977 88 12 38 2n=50 Sofradzija et al. , 1979 96 10 24 16 2n=50 Rab, 1981 - - - - 2n=50 Collares -Perier a, 1989 96 -92 8-4 28 -24 16 -12 2n=50 Rab & Collares -Periera, 1995 90 - - - 2n=50 Gul et al., 2004 88 12 22 16 2n=50 Kilic -Demürok & Unlu, 2004

. A. bipunctatus Kilic-Demürok & ) NF 96= NF =88 . (Unlu, 2004 . 1386 . .. NF 96= Garra rufa (Actinopterygii: Cyprinidae) . ( 1995 ) Collares-Periera Rab ( 1981 ) Rab . 17. 11 1386 3 Levan .1386 . . ( 1964 ) ( Chalcalburnus chalcoides ) ) .( Vimba vimba persa ) ( .( Vimba vimba persa .) . .1384 . .( Hemiculter leucisculus )

Downloaded from isfj.ir at 9:09 +0330 on Thursday November 30th 2017 [ DOI: 10.22092/ISFJ.2017.110126 ] . . . ... Barbus . 1384 . Capoeta Barbus mursa, capito 58 . capoeta . 842 831 4 . . . . 1389 ..

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and fish chromosomes. Ljubljana, 97P. (. Vimba vimba persa ) Cataudella S., Sola L., Accame Muratori R. and 30. 19 2 Capanna E., 1977. The chromosomes of 11 .1381 .. . species of Cyprinidae and one Cobitdae from Schizothorax zarudnyi Italy, with some remarks on the problem of 2 . polyploidy in the cypriniformes. Genetica, 21. 13 1 47:161-171. . . . 1378 . Collares-Pereira M.J., 1989. Hybridization in . 378 European Cyprinids: Evolutionary potential of .1380 . . . unisexual populations. In : Dawley RM (ed). Aristichthys ) Bulletin 466, New York, USA, pp.281-287. . ( nobilis Esmaeili H.R., Ebrahimi M., Ansari T.H., . Teimory A. and Gholamhosseini G.A., 2009. . 1383 . . Karyotype analysis of Persian stone lapper, .( ) Garra persica Berg, 1913 (Actinopterygii: . 80 . Cyprinidae) from . Current Sciences, . 1381 .. . .. 96(7):959-962. Ctenopharyngodon idella Esmaeili H.R. and Piravar Z., 2006. Karyotype of . Persian Chub, Petroleuciscus persidis (Coad, 1381 3 1981) (Actinopterygii: Cyprinidae) from .144 137 Southern Iran. Turkish Journal of Zoology, .1374 . . . 30:137-139. Esmaeili H.R., Zareian H., Gholamhosseini A., 1 . Ebrahimi M., Gholami Z., Teimori A. and 71. 64 1374 Ansari T.H., 2010. Karyotype Analysis of the .1380 . . .

Downloaded from isfj.ir at 9:09 +0330 on Thursday November 30th 2017 [ DOI: 10.22092/ISFJ.2017.110126 ] King Nase Fish, Chondrostoma regium (Heckel, Abramis brama 1843) (Actinopterygii: Cyprinidae) from Iran. 3 . Turkish Journal of Fisheries and Aquatic . 100 89 Sciences, 10:477-481. . . . Gold J.R., Li Y.C., Shipley N.S. and Powers P.K., .1381 .. 1990. Improved methods for working with fish Hypophthamichthys molitrix chromosomes with a review of metaphase . chromosome banding. Journal of Fish Biology, .114 107 1381 1 37:563-575. Al-Sabti K. , 1991. Hand book of genotoxic effects 143 ( Alburnoides bipunctatus )

Gul S., Colak A., Sezgin I. and Kaloglu B., 2004. Lakara W.S., John G. and Barat A., 1997. Karyotype analysis in Alburnus heckeli Cytogenetic studies on endangered and (Battalgil, 1943) from Lake Hazer. Turkish threatened fishes karyotypes of two species of Journal of Veterinary and Science, Snow-trout, Schizothorax richardsonii (Gray) 28:309-314. and S. kumaonensis (Menon). Proceedings of the Kalbassi M.R., Hosseini S.V. and Tahergorabi R., National Academy of Sciences India, section B: 2008. Karyotype analysis in Schizothorax Biological Science, 67(1):79-81. zarudnyi from Hamoon Lake, Iran. Turkish Levan A., Fredga K. and Sandberg A.A., 1964 . Journal of Fisheries and Aquatic Sciences, Nomenclature for centromeric positions on 8:335-340. chromosomes. Hereditas, 52:201-202. Kilic-Demürok N. and Unlu E., 2001. Karyotypes Nazari S., Pourkazemi M. and Porto J.I.R., 2011. of Cyprinid fish Capoeta trutta and Capoeta Chromosome description and localization of capoeta umbla (Cyprinidae) from the Tigris nucleolus organizing regions (NORs) by Ag- River . Turkish Journal of Zoology, 25:389-393 . staining technique in Alburnus filippii Kilic-Demürok N. and Unlu E., 2004. Karyotypes (Cyprinidae, Cypriniformes) in Anzali Lagoon, of the Cyprinid fish Alburnoides bipunctatus North Iran. Iranian Journal of Fisheries Science, (Cyprinidae) from the Tigris River. Folia 10(2):352-355. Biologica (Krakow), 52:57-59 . Oellerman L.K. and Skelton P.H., 1990. Khosravanizadeh A., Pourkazemi M. and Hexaploidy in yellofish species (Barbus, Pisces, Nowruzfashkhami M.R., 2011. Karyology Cyprinidae) from southern Africa. Journal of study on Bleak ( Alburnus alburnus ) from the Fish Biology, 37:105-115. south Caspian Sea region . Caspian Journal of Pourkazemi M., Nazari S. and Bakhshalizadeh S., Environmental Sciences, 9(1):27-36. 2010. Karyotype analysis in white bream ( Blicca Khuda-Bukhsh A.R., Chanda T. and Barat A., bjoerkna transcaucasica ) from north coast of 1986. Karyomorphology and evolution in some Iran. Iranian Journal of Fisheries Science,

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145 Iranian Scientific Fisheries Journal short communication Vol. 22, No. 2, Summer 2013

Karyology study of Spirlin ( Alburnoides bipunctatus ) in Zabol region

Khosravanizadeh A. (1)* ;Pourkazemi M. (2) ;Nowrozfashkhami M.R. (3) [email protected] 1- Institute of Hamoon international wetland of Zabol University, P.O.Box: 98615-538 Zabol, Iran 2,3- International Sturgeon Research Institute, P.O.Box: 41635-3464 Rasht, Iran Received: September 2011 Accepted: February 2011

Keywords: Zabol, Karyotype, Spirlin, Alburnoides bipunctatus

Abstract The chromosomal spread and karyotype of Spirlin ( Alburnoides bipunctatus ) from Zabol region were identified using tissue squashing techniques with injection of 0.5ml/100g body weight of 0.01% Colchicines solution in fish fingerlings. Kidney and gill tissues were extracted and chopped in KCl 0.075M for 30 min and fixed in Carnoy solution in 3 stages. The chromosomal spreads were stained in 20% Gimsa for 20 min. From 34 chromosomal spread counts, the results showed diploid chromosome number of this species 2n=50. Karyotype composed of 9 metacentric, 14 submetacentric and 2 acrocentric or subtelocentric chromosome pairs, and the number of chromosome arms (NF) was determined as NF=96.

*Corresponding author Downloaded from isfj.ir at 9:09 +0330 on Thursday November 30th 2017 [ DOI: 10.22092/ISFJ.2017.110126 ]

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