Pcr Primer Design for 16S Rrnas for Experimental Horizontal Gene Transfer Test in Escherichia Coli

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Pcr Primer Design for 16S Rrnas for Experimental Horizontal Gene Transfer Test in Escherichia Coli View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Frontiers - Publisher Connector ORIGINAL RESEARCH published: 28 February 2017 doi: 10.3389/fbioe.2017.00014 PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli Kentaro Miyazaki1,2*, Mitsuharu Sato1,2 and Miyuki Tsukuda1,2 1 Department of Life Science and Biotechnology, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan, 2 Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan We recently demonstrated that the Escherichia coli ribosome is robust enough to accommodate foreign 16S rRNAs from diverse gamma- and betaproteobacteria bac- teria (Kitahara et al., 2012). Therein, we used the common universal primers Bac8f and UN1541r to obtain a nearly full-length gene. However, we noticed that these primers overlap variable sites at 19[A/C] and 1527[U/C] in Bac8f and UN1541r, respectively, and thus, the amplicon could contain mutations. This is problematic, particularly for the former site, because the 19th nucleotide pairs with the 916th nucleotide, which is Edited by: a part of the “central pseudoknot” and is critical for function. Therefore, we mutation- Diana Elizabeth Marco, ally investigated the role of the base pair using several 16S rRNAs from gamma- and National Scientific Council (CONICET), Argentina betaproteobacteria. We found that both the native base pairs (gammaproteobacterial Reviewed by: 19A–916U and betaproteobacterial 19C–916G) and the non-native 19A–916G pair Kei Kitahara, retained function, whereas the non-native 19C–916U was defective 16S rRNAs. We Hokkaido University, Japan Trevor Carlos Charles, next designed a new primer set, Bac1f and UN1542r, so that they do not overlap the University of Waterloo, Canada potential mismatch sites. 16S rRNA amplicons obtained from the environmental metag- *Correspondence: enome using the new primer set were dominated by proteobacterial species (~85%). Kentaro Miyazaki Subsequent functional screening identified various 16S rRNAs from proteobacteria, all [email protected] of which contained native 19A–916U or 19C–916G base pairs. The primers developed Specialty section: in this study are thus advantageous for functional characterization of foreign 16S rRNA This article was submitted to in E. coli with no artifacts. Microbiotechnology, Ecotoxicology and Bioremediation, Keywords: bacterial phylogeny, 16S rRNA, ribosome, horizontal gene transfer, molecular clock, functional a section of the journal complementation, metagenome, central pseudoknot Frontiers in Bioengineering and Biotechnology Received: 19 December 2016 INTRODUCTION Accepted: 09 February 2017 Published: 28 February 2017 The bacterial ribosome consists of 3 rRNA molecules and 54 proteins and plays a crucial role in Citation: translating mRNA-encoded information into proteins. Because of the structural complexity of Miyazaki K, Sato M and Tsukuda M the ribosome (Schuwirth et al., 2005), it is believed that each ribosomal component coevolves to (2017) PCR Primer Design for 16S rRNAs for Experimental Horizontal maintain function (Jain et al., 1999). In particular, because the 16S and 23S rRNAs form the struc- Gene Transfer Test in Escherichia coli. tural core of the ribosome (Schuwirth et al., 2005), they are believed to be least likely to experience Front. Bioeng. Biotechnol. 5:14. horizontal gene transfer between species (Jain et al., 1999). On the basis of the species-specific nature doi: 10.3389/fbioe.2017.00014 of rRNA and their omnipresence in all bacteria, the rRNA genes, especially those for 16S rRNA, have Frontiers in Bioengineering and Biotechnology | www.frontiersin.org 1 February 2017 | Volume 5 | Article 14 Miyazaki et al. Functional Analysis of 16S rRNA long been used as an “ultimate chronometer” (Woese, 1987) for phylogenetic classification of bacterial species (Lane et al., 1985; Woese, 1987). Despite the apparent species-specific nature of 16S rRNAs, we recently found that the Escherichia coli ribosome is able to accommodate foreign 16S rRNA (Kitahara et al., 2012). Namely, using E. coli Δ7, a null mutant of the rrn (ribosomal RNA) operon, as a host strain, we have shown that various 16S rRNA genes, including those from a different phyloge- netic class (i.e., betaproteobacteria), were able to complement growth. The lowest identity of functional 16S rRNA gene to that of E. coli was as low as 80%, implying that hundreds of simultaneous nucleotide changes are permitted in the mainte- nance of ribosome function. The basis for this high mutability is the conservation of the RNA secondary structures, which is consistent with a previous finding that 16S rRNA is typically recognized by ribosomal proteins via salt bridges between phosphate oxygen atoms of the RNA backbone, but nucleotide bases are not strictly discriminated (Brodersen et al., 2002). Furthermore, insertion/deletion is allowed in some RNA helices (e.g., h6, 10, and 17) that are not involved in protein binding. Understanding the sequence and structural variations of 16S rRNA that are accommodated in the E. coli ribosome should be helpful for our understanding of the evolution of rRNA and the sequence–structure–function relationships of the ribosome. In our previous study, to PCR amplify foreign 16S rRNA genes, we used Bac8f(A) or Bac8f(C) [the most commonly used “Bac8f” (Eden et al., 1991)] for the forward primer and UN1541r(U) or UN1541r(C) for the reverse primer (Figure 1; oligonucleotide sequences summarized in Table 1) (Kitahara et al., 2012), which allowed amplification of a nearly full-length gene. These primers can cover the majority of bacterial 16S FIGURE 1 | Coverage rates and nucleotide composition at the (A) rRNA genes and thus are commonly used for phylogenetic and/ 5′- and (B) and 3′-ends of the bacterial 16S rRNA gene. The annealing regions for Bac1f, Bac8f, and UN1541r are shown by arrows with the or community analysis (Lane et al., 1985; Weisburg et al., 1991; sequences. Wobble nucleotides are marked by asterisks. Colors: blue, A; Amann et al., 1995). However, we noticed that the amplicons red, T; green, G; purple, C; black, gap. obtained using the primer set contained mutations at certain frequencies, which could affect the functionality of in vivo- reconstituted mutant ribosomes. In E. coli 16S rRNA, nucleo- tides 17–19 pair with nucleotides 916–918 to form a short helix take the RNA processing issue into consideration. For proper (h2) (Figure 2). The helix is involved in the formation of the processing of a precursor transcript into mature rRNAs (16S, “central pseudoknot,” whose structure is highly conserved in 23S, and 5S rRNAs), the processing sites (i.e., RNase cleavage both prokaryotes and eukaryotes. This unique structure is sites) need to be similar to the E. coli sequences (Gutgsell and essential for translational initiation and is highly susceptible Jain, 2012). to point mutations (Brink et al., 1993; Dammel and Noller, Taking these points into consideration, we first evaluated the 1993; Poot et al., 1998). Despite this structural conservation, effects of mismatches between the 19th and 916th nucleotides. In however, the 19th nucleotide varies depending on the species, addition to the native 19A–916U and 19C–916G pairs, the non- 19A or 19C, which pairs with 916U or 916G, respectively native 19A–916G pair retained function, whereas the non-native (Figure 2). Thus, if Bac8f(A) or Bac8f(C) is used as a primer, 19C–916U was detrimental. Second, we designed new primers, there is a possibility of generating a mismatch between the 19th Bac1f and UN1542r, which encompassed nucleotide positions and 916th nucleotides. Similarly, the 1,527th position is also 1–18 for Bac1f and 1542–1528 for UN1542r, so that they did not variable (C or U) and can generate a mismatch in the amplicons overlap the potential mismatch sites. These primers were used for (Figure 1B), although this site may not be involved in function. PCR amplification of 16S rRNA genes and the resultant library Thus, in our specific system for functional investigation of 16S was functionally screened. DNA sequencing of the 16S rRNA rRNAs, it is essential to develop a new primer set to avoid the genes in the functional clones confirmed the absence of unwanted introduction of artificial mutations. In addition, we need to mismatches in the functional genes. Frontiers in Bioengineering and Biotechnology | www.frontiersin.org 2 February 2017 | Volume 5 | Article 14 Miyazaki et al. Functional Analysis of 16S rRNA TABLE 1 | PCR primers used for amplification of 16S rRNA genes. Primer Sequence Reference Bac1f 5′-AAATTGAAGAGTTTGATC-3′ This study Bac8f(A)a 5′-AGAGTTTGATCATGGCTCAG-3′ Kitahara and Miyazaki (2011); Kitahara et al. (2012); Weisburg et al. (1991) Bac8f(C)a 5′-AGAGTTTGATCCTGGCTCAG-3′ Kitahara and Miyazaki (2011); Kitahara et al. (2012); Weisburg et al. (1991) UN1542r 5′-TAAGGAGGTGATCCA-3′ This study UN1541r(U)a 5′-AAGGAGGTGATCCAACC-3′ Kitahara and Miyazaki (2011); Kitahara et al. (2012) UN1541r(C)a 5′-AAGGAGGTGATCCAGCC-3′ Kitahara and Miyazaki (2011); Kitahara et al. (2012); Weisburg et al. (1991) Bac1r 5′-GATCAAACTCTTCAATTTAAAAGTTTGACGCTCAAAG-3′ This study Bac8r(A) 5′-CTGAGCCATGATCAAACTCTTC-3′ Kitahara and Miyazaki (2011); Kitahara et al. (2012) Bac8r(C) 5′-CTGAGCCAGGATCAAACTCTTC-3′ This study UN1542f 5′-TGGATCACCTCCTTACCTTAAAGAAGCGT-3′ This study aUnderlined
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