The New Eppendorf Micro Centrifuge
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Pnas11148toc 3..7
December 2, 2014 u vol. 111 u no. 48 u 16975–17336 Cover image: Pictured is a vermillion sea star, Mediaster aequalis,inPorlierPass,British Columbia, that shows signs of sea-star wasting disease. As of June 2014, the disease had affected 20 species of sea stars from Alaska to Baja California, but its cause is unknown. Ian Hewson et al. surveyed sea-star populations and conducted laboratory infection studies. They found that sea-star wasting disease is likely caused by a virus and identified a densovirus as a potential infectious agent. See the article by Hewson et al. on pages 17278–17283. Image courtesy of Peter Luckham (www.divemaster.ca). From the Cover 17278 Potential cause of sea-star wasting disease 17075 Human ability to evaluate probabilities 17122 X-ray free crystallography 17140 DNA binding site recognition by regulatory proteins 17182 Ebola antibodies’ modes of action Contents COMMENTARIES 16982 Going viral and the fatal vulnerability of neurons from immunity, not from infection THIS WEEK IN PNAS Lawrence Steinman See companion article on page 16053 in issue 45 of volume 111 16975 In This Issue 16984 Fuzzy universality of probability judgment Valerie F. Reyna and Charles J. Brainerd See companion article on page 17075 INNER WORKINGS—An over-the-shoulder look at scientists at work 16986 Expanding the femtosecond crystallography toolkit Sol M. Gruner 16977 Inner Workings: Freeing the dinos within See companion article on page 17122 Stephen Ornes CORE CONCEPTS—A brief introduction to emerging topics in science PNAS PLUS 16978 Core Concept: Synthetic biology—change, accelerated 16988 Significance Statements Danielle Venton Brief statements written by the authors about the significance of their papers. -
Documenting the Biotechnology Industry in the San Francisco Bay Area
UC San Diego Reports and Studies Title Documenting the Biotechnology Industry in the San Francisco Bay Area Permalink https://escholarship.org/uc/item/1m24k447 Author Chandler, Robin L. Publication Date 1997 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Documenting the Biotechnology Industry In the San Francisco Bay Area Robin L. Chandler Head, Archives and Special Collections UCSF Library and Center for Knowledge Management 1997 1 Table of Contents Project Goals……………………………………………………………………….p. 3 Participants Interviewed………………………………………………………….p. 4 I. Documenting Biotechnology in the San Francisco Bay Area……………..p. 5 The Emergence of An Industry Developments at the University of California since the mid-1970s Developments in Biotech Companies since mid-1970s Collaborations between Universities and Biotech Companies University Training Programs Preparing Students for Careers in the Biotechnology Industry II. Appraisal Guidelines for Records Generated by Scientists in the University and the Biotechnology Industry………………………. p. 33 Why Preserve the Records of Biotechnology? Research Records to Preserve Records Management at the University of California Records Keeping at Biotech Companies III. Collecting and Preserving Records in Biotechnology…………………….p. 48 Potential Users of Biotechnology Archives Approaches to Documenting the Field of Biotechnology Project Recommendations 2 Project Goals The University of California, San Francisco (UCSF) Library & Center for Knowledge Management and the Bancroft Library at the University of California, Berkeley (UCB) are collaborating in a year-long project beginning in December 1996 to document the impact of biotechnology in the Bay Area. The collaborative effort is focused upon the development of an archival collecting model for the field of biotechnology to acquire original papers, manuscripts and records from selected individuals, organizations and corporations as well as coordinating with the effort to capture oral history interviews with many biotechnology pioneers. -
Hria Medical Foundation 2012 Review
20․12 DI VISION REVIE W Where Science and Philanthropy Converge IN THIS ISSUE: About Us and Our Services / 2 Funding Opportunities Jeffress Trust /8 Hood Foundation /10 Noonan Memorial Research Fund /11 Klarman Family Foundation /12 King Trust /13 Thome Foundation /14 Smith Family Foundation /16 Davis Foundation /17 Lymphatic Research Foundation /18 Scientific Review Committees /19 Gene Discovery in Anorexia Nervosa / 6 The Medical Foundation, a division of HRiA About Us Since 1957, foundations, bank trusts and individuals have engaged us to create and manage customized biomedical research grant programs that accelerate the pace of scientific discoveries. As evidenced by the more than 145,000 visits to our website this year alone, our funding announcements reach thousands of potential applicants for every grant cycle. And, by building a distinguished Scientific Review Committee for each program, we ensure critical and unbiased selection of the best minds in science. In 2012, we were privileged to work with foundations and bank trust departments whose grant programs distributed more than $18 million to investigators and physician-scientists across the United States and worldwide. Sally E. McNagny, M.D., M.P.H., F.A.C.P., Vice President Since 2001, Dr. McNagny has served as Vice President and head of HRiA’s Medical Foundation division where she leads biomedical research grantmaking and life sciences consulting. Dr. McNagny also serves on the faculty at Harvard Medical School and is a Fellow of the American College of Physicians. She holds a B.S. in Biology from Stanford University, an M.D. from Harvard Medical School, an M.P.H. -
Prion Diseases in Knock-In Mice Carrying Single Prp Codon Substitutions Associated with Human Diseases
Profoundly different prion diseases in knock-in mice carrying single PrP codon substitutions associated with human diseases Walker S. Jacksona,b,c,1, Andrew W. Borkowskia,b,c, Nicki E. Watsona, Oliver D. Kingd, Henryk Faase, Alan Jasanoffe,f, and Susan Lindquista,b,c,2 aWhitehead Institute for Biomedical Research, Cambridge, MA 02142; bHoward Hughes Medical Institute, cDepartment of Biology, and fDepartments of Biological Engineering, Brain and Cognitive Sciences, and Nuclear Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139; dDepartment of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, MA 01655; and eFrances Bitter Magnet Laboratory, Cambridge, MA 02139 Contributed by Susan Lindquist, July 9, 2013 (sent for review March 7, 2013) In man, mutations in different regions of the prion protein (PrP) linked to it, provides an important general model for such are associated with infectious neurodegenerative diseases that investigations. have remarkably different clinical signs and neuropathological There are several types of human prion diseases, each begin- lesions. To explore the roots of this phenomenon, we created ning with pathologic processes in a different brain region and fi – a knock-in mouse model carrying the mutation associated with leading to distinct functional de cits: cognition [Creutzfeldt – – one of these diseases [Creutzfeldt–Jakob disease (CJD)] that was Jakob disease (CJD)], movement control (Gerstmann Sträussler Scheinker syndrome), or sleep and autonomic functions [fatal exactly analogous to a previous knock-in model of a different fl prion disease [fatal familial insomnia (FFI)]. Together with the familial insomnia (FFI)] (7). Prion diseases also af ict animals WT parent, this created an allelic series of three lines, each express- and include bovine spongiform encephalopathy (BSE) of cattle, scrapie of sheep and goats, and chronic wasting disease (CWD) ing the same protein with a single amino acid difference, and with of deer and elk (1). -
Structure & Symmetry
HAPPY HOLIDAYS ASBMB MEMBERS December 2008 Structure & Symmetry American Society for Biochemistry and Molecular Biology J\\PfliGifk\`ej n`k_*.#'''>=G$kX^^\[FI=Zcfe\j 8 $@C- 9 "@C- : ; >=G$kX^^\[Kil\FI=Zcfe\jXi\kiXej]\Zk\[`ekf?<B)0* Z\ccjXe[k_\kX^^\[gifk\`ejXi\m`jlXc`q\[[li`e^@C$- `e[lZ\[elZc\XikiXejcfZXk`feJK8K*#gXe\c8Xe[9 Xe[`e Ôcfgf[`XXe[jki\jjÔY\i]fidXk`fe8Zk`e#gXe\c:Xe[; % Kil\FI= Fi`>\e\jXclk\j >\efd\n`[\FI=Zcfe\j k_\>=Gg`fe\\ij ]fik_\`iEfY\c ]fikX^^\[gifk\`e\ogi\jj`fe Gi`q\XnXi[ ×:$k\id`eXckX^f]>=G ×J\hl\eZ\m\i`Ô\[Xe[^lXiXek\\[ ×<Xj`cpj_lkkc\[`ekf)'[\jk`eXk`fem\Zkfij ×KiXej]\Zk`fe$i\X[p1('l^gcXjd`[;E8 fi`^\e\%Zfd&fi] ORG-041-GFPTaggedAd_ASBMB_v7.indd 1 10/20/08 12:29:39 PM contents DECEMBER 2008 ON THE COVER: Captivated by the symmetry society news of molecular structure, Sung-Hou Kim has been a 2 From the Editor leader in revealing symmetry 3 President’s Message through his studies in crystallography and 5 Letters to the Editor structural genomics. 30 6 Washington Update 12 Retrospective: Anthony G. San Pietro FASEB releases new Breakthroughs in special interest Bioscience. 6 13 Science’s Role in Foreign Policy 14 ASBMB Round Table: Jim Wells and Mary Woolley 16 Keeping Women in Science 19 Grammar and Writing Tips 2009 meeting 20 The 2009 Fritz Lipmann Lectureship: Douglas C. Rees 21 The 2009 ASBMB Merck Award: John Kuriyan 22 The 2009 FASEB Excellence in Science Award: Susan Lindquist science focus 30 Sung-Hou Kim: Consummate Crystallographer departments 7 News from the Hill 10 Member Spotlight 23 Education and Training A leaky pipeline for women scientists. -
Susan Lee Lindquist (1949–2016)
In Memoriam Sue was a spectacular scientist who com- at the University of Chicago, where she bined a searing intellect with deep wis- would later join the faculty (1978) and rise Susan Lee Lindquist (1949–2016) dom, sagacious intuition, and limitless to full professor (1988). While at the Uni- creativity. These characteristics enabled versity of Chicago, Sue married Edward Sue to make connections across dispa- Buckbee and would have two wonderful 1, James Shorter * rate disciplines that nobody else could daughters, Alana and Nora. She also make. Her infectious esprit for scientific launched a remarkable and radical series Lindquist was a visionary and pio- discovery was combined with disarming of trailblazing discoveries. These contin- neer who transformed our under- warmth, positivity, openness, directness, ued when Sue moved her research pro- standing of how protein folding and generosity, which made her an inspi- gram to the Whitehead Institute for rational, nurturing, and indefatigable men- Biomedical Research at Massachusetts sculpts biology, evolution, and dis- tor. These synergistic traits empowered Institute of Technology (MIT), which is ease. She revealed several unantici- extraordinarily effective collaborations where I trained with Sue as a postdoctoral pated mechanisms by which protein between scientists from diverse back- fellow (2002–2007). Sue would spend the folding can buffer, release, and grounds and disciplines. Indeed, rest of her career at the Whitehead Insti- potentiate genetic variation in researchers from diverse backgrounds – tute as Director (2001–2004), Institute response to environmental stress, physicists, chemists, biochemists, biolo- Member (2001–2016), and Professor of thereby enabling the rapid evolution gists, mathematicians, and physicians – Biology at MIT (2001–2016). -
History of Digital PCR Trisha Dhawan and Rémi Dangla 8/8/19
History of Digital PCR Trisha Dhawan and Rémi Dangla 8/8/19 Outline 1.1. PCR, the start of a revolution .......................................................................................... 1 1.2. Limit dilution PCR .......................................................................................................... 1 1.3. Digital PCR, a term coined by Vogestein and Kinzler ................................................... 2 2.1. PCR meets microfluidics ................................................................................................ 2 2.2. PCR in micro-droplets .................................................................................................... 3 3.1. Digital PCR in Microarrays ............................................................................................ 4 3.2. Digital PCR in Micro-droplets ........................................................................................ 4 Combining microarrays and microdropelts with Crystal™ digital PCR ............................ 5 Discussion ........................................................................................................................... 6 The origins of Digital PCR of template nucleic acid molecules, oligonucleotide primers, dNTPs, and a 1.1. PCR, the start of a revolution thermostable DNA polymerase. Over the years, The polymerase chain reaction (PCR) was ongoing development and application of the PCR invented by Kary Mulis while he was developing reaction enabled molecular cloning, engineered methods for -
Calcineurin Determines Toxic Versus Beneficial Responses to Α-Synuclein
Calcineurin determines toxic versus beneficial responses to α-synuclein Gabriela Caraveoa,b, Pavan K. Aulucka,c,1, Luke Whitesella, Chee Yeun Chunga, Valeriya Barua,b, Eugene V. Mosharovd, Xiaohui Yane, Manu Ben-Johnyf, Martin Sosteg, Paola Picottig, Hanna Kime, Kim A. Caldwelle, Guy A. Caldwelle, David Sulzerd,h, David T. Yuef, and Susan Lindquista,b,2 aWhitehead Institute for Biomedical Research, Cambridge, MA 02142; bHoward Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139; cDepartment of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114; Departments of dNeurology and hPsychiatry, Columbia University Medical Center, New York, NY 10032; eDepartment of Biological Sciences, The University of Alabama, Tuscaloosa, AL 35487; fDepartments of Biomedical Engineering and Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD 21205; and gDepartment of Biology, Institute of Biochemistry, Eidgenossische Technische Hochschule Zurich, Zurich CH-8093, Switzerland Contributed by Susan Lindquist, July 15, 2014 (sent for review May 7, 2014) + Calcineurin (CN) is a highly conserved Ca2 –calmodulin (CaM)- tigations, given their genetic tractability and the remarkable + + dependent phosphatase that senses Ca2 concentrations and trans- conservation of Ca2 -signaling pathways from yeast to humans duces that information into cellular responses. Ca2+ homeostasis (14, 15). Moreover, the expression of human α-syn in yeast leads is disrupted by α-synuclein (α-syn), a small lipid binding protein to cellular pathologies directly relevant to neurons and PD, in- whose misfolding and accumulation is a pathological hallmark of cluding nitrosative stress (16, 17), defects in vesicle trafficking several neurodegenerative diseases. We report that α-syn, from (18–20), and faulty mitochondrial function (21, 22). -
Regional Oral History Office University of California the Bancroft Library Berkeley, California
Regional Oral History Office University of California The Bancroft Library Berkeley, California Program in Bioscience and Biotechnology Studies RONALD E. CAPE, M.B.A., Ph. D. BIOTECH PIONEER AND CO-FOUNDER OF CETUS Interviews Conducted by Sally Smith Hughes in 2003 Copyright © 2006 by The Regents of the University of California Since 1954 the Regional Oral History Office has been interviewing leading participants in or well-placed witnesses to major events in the development of northern California, the West, and the nation. Oral history is a method of collecting historical information through tape-recorded interviews between a narrator with firsthand knowledge of historically significant events and a well-informed interviewer, with the goal of preserving substantive additions to the historical record. The tape recording is transcribed, lightly edited for continuity and clarity, and reviewed by the interviewee. The corrected manuscript is indexed, bound with photographs and illustrative materials, and placed in The Bancroft Library at the University of California, Berkeley, and in other research collections for scholarly use. Because it is primary material, oral history is not intended to present the final, verified, or complete narrative of events. It is a spoken account, offered by the interviewee in response to questioning, and as such it is reflective, partisan, deeply involved, and irreplaceable. ************************************ All uses of this manuscript are covered by legal agreements between The Regents of the University of California and Ronald Cape, dated December 18, 2003. The manuscript is thereby made available for research purposes. All literary rights in the manuscript, including the right to publish, are reserved to The Bancroft Library of the University of California, Berkeley. -
Documenting the Biotechnology Industry in the San Francisco Bay Area
Documenting the Biotechnology Industry In the San Francisco Bay Area Robin L. Chandler Head, Archives and Special Collections UCSF Library and Center for Knowledge Management 1997 1 Table of Contents Project Goals……………………………………………………………………….p. 3 Participants Interviewed………………………………………………………….p. 4 I. Documenting Biotechnology in the San Francisco Bay Area……………..p. 5 The Emergence of An Industry Developments at the University of California since the mid-1970s Developments in Biotech Companies since mid-1970s Collaborations between Universities and Biotech Companies University Training Programs Preparing Students for Careers in the Biotechnology Industry II. Appraisal Guidelines for Records Generated by Scientists in the University and the Biotechnology Industry………………………. p. 33 Why Preserve the Records of Biotechnology? Research Records to Preserve Records Management at the University of California Records Keeping at Biotech Companies III. Collecting and Preserving Records in Biotechnology…………………….p. 48 Potential Users of Biotechnology Archives Approaches to Documenting the Field of Biotechnology Project Recommendations 2 Project Goals The University of California, San Francisco (UCSF) Library & Center for Knowledge Management and the Bancroft Library at the University of California, Berkeley (UCB) are collaborating in a year-long project beginning in December 1996 to document the impact of biotechnology in the Bay Area. The collaborative effort is focused upon the development of an archival collecting model for the field of biotechnology to acquire original papers, manuscripts and records from selected individuals, organizations and corporations as well as coordinating with the effort to capture oral history interviews with many biotechnology pioneers. This project combines the strengths of the existing UCSF Biotechnology Archives and the UCB Program in the History of the Biological Sciences and Biotechnology and will contribute to an overall picture of the growth and impact of biotechnology in the Bay Area. -
Invited Keynote Speakers, Chairs and Vice Chairs
Invited Keynote Speakers, Chairs and Vice Chairs Session 1 - Viruses: From Environments to Clinics Tuesday, June 19th, 2018 Keynote Speaker: Dr. Peter Palese, Mount Sinai, New York, USA Towards a Universal Influenza Virus Vaccine Dr. Peter Palese is a Professor of Microbiology and the Chair of the Department of Microbiology at the Icahn School of Medicine at Mount Sinai. His research is in the area of RNA-containing viruses with a special emphasis on influenza viruses. Specifically, he established the first genetic maps for influenza A, B, and C viruses, identified the function of several viral genes, and defined the mechanism of neuraminidase inhibitors (which are now FDA-approved antivirals). He was also a pioneer in the field of reverse genetics for negative strand RNA viruses, which allows the introduction of site-specific mutations into the genomes of these viruses. Chair: Dr. Keith Fowke, University of Manitoba, Winnipeg, MB Dr. Keith Fowke is Professor and Head in the Department of Medical Microbiology and Infectious Diseases, University of Manitoba. His laboratory focuses on defining cellular immune mechanisms of the control of, and resistance to, HIV infection. Current studies include understanding how to block the negative effects of HIV to restore the immune response to full capabilities and preventing HIV infections by reducing inflammation at the genital tract. Vice-Chair: Dr. Peter Pelka, University of Manitoba, Winnipeg, MB Dr. Peter Pelka is an Assistant Professor at University of Manitoba. My lab studies how a viral oncoprotein reprograms the cell in order to support virus replication. I use the human adenovirus as a model system to study how E1A reprograms the infected cell. -
Maize Genetics Cooperation Newsletter Exists for the Benefit of the Maize Community As an Informal Vehicle for Communication
MAIZE GENETICS COOPERATION NEWSLETTER 80 July 25, 2006 The data presented here are not to be used in publications without the consent of the authors. Division of Biological Sciences and Division of Plant Services University of Missouri Columbia, Missouri The Maize Genetics Executive Committee Sarah Hake, Chair, Class of 2008 Marty Sachs, Class of 2010 Patrick Schnable, Class of 2010 Mary Schaeffer (Polacco), Class of 2009 Anne Sylvester, Class of 2009 Jo Messing, Class of 2008 Ed Buckler, Class of 2007 Karen Cone, Class of 2007 Alfons Gierl, Class of 2007 Jeanne-Philippe Vielle-Calzada, Class of 2007 Jeff Bennetzen, Class of 2006 Ron Phillips, Class of 2006 Year 2007 Maize Genetics Conference Steering Committee Anne Sylvester, Chair Thomas P. Brutnell, Co-Chair Ed Buckler Mei Guo Erin Irish Steve Moose Jorge Nieto Sotelo Peter Rogowski Richard Schneeberger Marja Timmermans Ex Officio Karen Cone, Treasurer Marty Sachs Mary Schaeffer (Polacco) Trent Seigfried NOTE: The 49th Maize Meeting will be held at St. Charles, IL, March 22-25, 2007. I. FOREWORD .......................................................................................................................................................................................................................1 II. REPORTS FROM COOPERATORS............................................................................................................................................................................2 BEIJING, CHINA Effect of space on leaf cell plasmadesma in maize (Zea mays L.)