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New IBD Genes? Gut: First Published As 10.1136/Gut.2004.062406 on 11 July 2005

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New IBD Genes? Gut: First Published As 10.1136/Gut.2004.062406 on 11 July 2005 1060 CLINICAL @LERT New IBD genes? Gut: first published as 10.1136/gut.2004.062406 on 11 July 2005. Downloaded from D McGovern, T Ahmad ............................................................................................................................... New reports of potential novel inflammatory bowel disease (IBD) genes further advance our understanding of the genetic basis of IBD rohn’s disease (CD) and ulcerative colitis result from an inappropri- Peltekova VD, Wintle RF, Rubin LA, et al. Functional variants of OCTN cation transporter Cate response of the mucosal genes are associated with Crohn disease. Nat Genet 2004;36:471–5. immune system to the normal enteric flora in a genetically susceptible indivi- Crohn disease is a chronic, inflammatory disease of the gastrointestinal tract. A locus dual. The importance of genetic predis- of approximately 250 kb at 5q31 (IBD5) was previously associated with suscept- position was firmly established in 2001 ibility to Crohn disease, as indicated by increased prevalence of a risk haplotype of by the identification of the first CD 11 single-nucleotide polymorphisms among individuals with Crohn disease, but the susceptibility gene NOD2 (CARD15)on pathogenic lesion in the region has not yet been identified. We report here that two chromosome 16q12 (IBD1).12 It is now variants in the organic cation transporter cluster at 5q31 (a missense substitution in clear that NOD2 variants contribute only SLC22A4 and a GRC transversion in the SLC22A5 promoter) form a haplotype a small part to genetic susceptibility, associated with susceptibility to Crohn disease. These variants alter transcription and suggesting the existence of other, as yet transporter functions of the organic cation transporters and interact with variants in unidentified, genes. Although these another gene associated with Crohn disease, CARD15, to increase risk of Crohn have remained elusive, linkage studies disease. These results suggest that SLC22A4, SLC22A5 and CARD15 act in a have provided important clues to their common pathogenic pathway to cause Crohn disease. location, implicating at least seven other regions of the human genome. Two recent studies report the cloning Stoll M, Corneliussen B, Costello CM, et al. Genetic variation in DLG5 is associated with of potential novel inflammatory bowel inflammatory bowel disease. Nat Genet 2004;36:476–80. disease (IBD) genes within linkage areas 3 4 on chromosomes 5 and 10. Linkage Crohn disease and ulcerative colitis are two subphenotypes of inflammatory bowel between CD and chromosome 5q31-33 disease (IBD), a complex disorder resulting from gene-environment interaction. We 5 (IBD5) was first demonstrated in 1999 refined our previously defined linkage region for IBD on chromosome 10q23 and and further characterisation of this locus used positional cloning to identify genetic variants in DLG5 associated with IBD. refined the region to a highly conserved DLG5 encodes a scaffolding protein involved in the maintenance of epithelial 6 http://gut.bmj.com/ 250 kb haplotype. Identifying the IBD5 integrity. We identified two distinct haplotypes with a replicable distortion in causal mutation(s) has been hampered transmission (P = 0.000023 and P = 0.004 for association with IBD, P = 0.00012 and by the degree of linkage disequilibrium P = 0.04 for association with Crohn disease). One of the risk-associated DLG5 and the density of immunoregulatory haplotypes is distinguished from the common haplotype by a nonsynonymous single- genes on this haplotype. A Canadian nucleotide polymorphism 113GRA, resulting in the amino acid substitution R30Q in 3 group now suggest that the true IBD5 the DUF622 domain of DLG5. This mutation probably impedes scaffolding of DLG5. disease causing mutations occur in We stratified the study sample according to the presence of risk-associated CARD15 SLC22A4 and SLC22A5, encoding OCTN1 variants to study potential gene-gene interaction. We found a significant difference on September 24, 2021 by guest. Protected copyright. and OCTN2 respectively, members of the in association of the 113A DLG5 variant with Crohn disease in affected individuals organic cationic transporter subfamily. carrying the risk-associated CARD15 alleles versus those carrying non-risk- These proteins are involved in the elim- associated CARD15 alleles. This is suggestive of a complex pattern of gene-gene ination of toxins and the uptake of interaction between DLG5 and CARD15, reflecting the complex nature of polygenic various physiological substrates, includ- diseases. Further functional studies will evaluate the biological significance of DLG5 ing carnitine (required for oxygen burst variants. mediated pathogen killing). OCTN1 and OCTN2 are widely expressed, including in intestinal epithelial cells, macro- phages, and T cells. Two putatively the risk haplotype is associated with a Precisely how these SNPs might interact functional single nucleotide polymorph- 3–4-fold risk of disease, similar to that with NOD2 to increase the risk of CD isms (SNPs), one in SLC22A4 and the for possession of CARD15 mutations. remains unclear. However, tantalising other in SLC22A5, form a risk haplotype Interestingly, the risk was much greater clues are provided by the suggestion that enriched in individuals with CD. The in the presence of both the risk haplotype a-defensins are potential substrates of L503F (leucine to phenylalanine at codon and CD associated CARD15 alleles, con- OCTN1 and 2, and by a recent report 503) polymorphism maps to a region of sistent with an interaction between IBD5 suggesting that NOD2 variants may actu- SLC22A4, important for cellular transport, and CARD15. The strong linkage ally increase susceptibility to CD, through and functional data presented by the disequilibrium across IBD5 makes inter- adefectina-defensin production.7 The authors suggest the variant allele may pretation of association data from indivi- same group has published a study in an affect uptake of carnitine, various xeno- dual SNPs difficult. This region is rich in independent cohort confirming their biotics, and toxins. The SLC22A5 SNP is genes, many of which are attractive findings. Analyses in this cohort have located in the gene promoter where it candidates for disease pathogenesis. It revealed particular association with ileal appears to disrupt a predicted heat shock therefore remains possible that other CD8 in contrast to a previous study element required for the binding of heat ‘‘functional’’ SNPs, expressed in tissues suggesting an association between IBD5 shock transcription factors. Possession of relevant to IBD, exist on this haplotype. and perianal CD.9 www.gutjnl.com CLINICAL @LERT 1061 A second study reports an association The rapid advances in our under- with susceptibility to Crohn’s disease. Nature between SNPs in DLG5, a member of the 2001;411:599–603. standing of the genetic basis of IBD 2 Ogura Y, Bonen DK, Inohara N, et al. A membrane associated guanylate kinase are yet to impact on routine clinical frameshift mutation in NOD2 associated with Gut: first published as 10.1136/gut.2004.062406 on 11 July 2005. Downloaded from gene family, and IBD. A German group4 practice although recent discoveries susceptibility to Crohn’s disease. Nature performed linkage disequilibrium map- have provided considerable insights for 2001;411:603–6. 3 Peltekova VD, Wintle RF, Rubin LA, et al. ping of a locus on chromosome 10 and investigators. There is considerable opti- Functional variants of OCTN cation transporter demonstrated association with variants mism that as IBD genes are identified genes are associated with Crohn disease. Nat in DLG5. Four common haplotypes and their environmental interactions Genet 2004;36:471–5. 4 Stoll M, Corneliussen B, Costello CM, et al. across DLG5 were identified. Haplotype unravelled that we may be able to better Genetic variation in DLG5 is associated with D (containing the unique SNP 113A) define the heterogeneous group of inflammatory bowel disease. Nat Genet conferred a modest risk of CD while patients with IBD, aid understanding 2004;36:476–80. haplotype A was protective. The results of the molecular mechanisms specific to 5 Ma Y, Ohmen JD, Li Z, et al. A genome-wide search identifies potential new susceptibility loci were replicated in a case control study subgroups of disease, and provide a for Crohn’s disease. Inflamm Bowel Dis (but not in an independent family based framework to predict clinical phenotype 1999;5:271–8. association study) and once again pos- and response to therapy. These novel 6 Rioux JD, Daly MJ, Silverberg MS, et al. Genetic variation in the 5q31 cytokine gene cluster confers sible interaction with the CD associated findings reported by the Canadian and susceptibility to Crohn disease. Nat Genet CARD15 alleles was noted. DLG5 is German make an important contribu- 2001;29:223–8. expressed in the colon and small intes- tion towards this goal. 7 Wehkamp J, Harder J, Weichenthal M, et al. NOD2 (CARD15) mutations in Crohn’s disease tine where it acts as a multifunctional are associated with diminished mucosal alpha- adapter and scaffold protein involved in Gut 2005;54:1060–1061. defensin expression. Gut 2004;53:1658–64. the regulation of epithelial cell growth, doi: 10.1136/gut.2004.048918 8 Newman B, Gu X, Wintle R, et al. A risk shape, and polarity. Computer predic- haplotype in the Solute Carrier Family 22A4/ .................. 22A5 gene cluster influences phenotypic tion programs suggest that the IBD Authors’ affiliations expression of Crohn’s
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