QUALITY MEANS TRUST

β-Glucuronidase: An E ctive Sample Preparation for Drugs of Abuse Screening and Therapeutic Drug Monitoring SUPPORTING ALL TESTING NEEDS WITH THE 600 BROADEST ENZYME OFFERING IN THE INDUSTRY 500 β-Glucuronidase is a key enzymatic component for sample Sigma400 -Aldrich® has over 60 years of experience as a primary preparation of metabolites of several drug classes in human manufa300 cturer of β-Glucuronidase o ering industry leading quality biolog s. C your analytical results and wor ow management200 and consistency of supply.

cy is dependent on quality reagents in each and every step. 100 Concentration (ng/mL) 0 Spend Your Lab’s Time E ectively 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 Time (hrs) Our exclusive Limpet sourced β-Glucuronidase provides high rates of hydrolysis and br ty across key drug classes. Limpet Abalone Competitor Recombinant Hydrolysis should be optimized f s. Oxymorphone Glucuronide Hydrolysis Curve Oxymorphone Glucuronide Rate of Hydrolysis

600 14

500 12

400 10 300 8 200 6

100 ng/mL/min Concentration (ng/mL) 4 0 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2 Time (hrs) 0 Competitor Abalone Limpet Limpet Abalone Competitor Recombinant Recombinant Glucuronide substrates spiked at 1000 ng/mL in synthetic urine. All titers were normalized using the industry standard phenolphthalein-glucuronide colorimetric assay at 37 °C. Enzyme titers were 10 U/µL and 1 U/µL spiked urine for codeine and oxymorphone, respectively. Samples were quanti by LC/MSMS using heavy isotope labelled parent compound internal standards.

14250 Commercial Crude ß-Gluc 6-M600A M to Morphine Conversion 12 200 Comme500 rcial crude enzyme products can be plagued by the 10 co-e400xtra ction of esterase activity that results in unwanted conversion 150 8 of 6-300M onoacetylmorphine (6-MAM) to morphine, confusing toxicology200 results. 1006 ng/mL/min oncentrations (μg/mL) New100, highly pur greatly reduces this unwanted enzyme

4 Concentration (ng/mL) 50 activit0y and provides a high activity protein. 2 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 0 Time (hrs) Morphine C 0 0 0.5 1 1.5 2 Competitor Abalone Limpet Recombinant Time (hrs) Limpet Abalone Competitor Recombinant

8 600 7 500 Concentration (ng/mL) Rely on Sigma-Aldrich® to be Your Trusted Partner

Are you looking to increase your eciency in sample preparation for Do you have specic packaging, formulation, or large volume/ drug screening or therapeutic drug monitoring analysis? reserved supply needs?

β-Glucuronidase from Molluskan Source Cat. No. Form Product Description Glucuronidase Activity Sulfatase Activity Highly Puri ed Abalone SRE0022 Solid β-Glucuronidase from abalone entrails NEW ≥20,000,000 units/g solid ≤4,000,000 units/g solid SRE0023 Liquid β-Glucuronidase from abalone entrails NEW ≥100,000 units/mL ≤7,500 units/mL Abalone Source SRE0038 Solid β-Glucuronidase from abalone entrails NEW 1,500,000–3,000,000 units/g solid ≤100,000 units/g SRE0037 Liquid β-Glucuronidase from abalone entrails NEW ≥100,000 units/mL ≤8,000 units/mL Limpet Source G8132 Solid β-Glucuronidase from limpets (Patella vulgata) Type L-II 1,000,000–3,000,000 units/g solid ≥1,000 units/g solid G2174 Liquid β-Glucuronidase from limpets (Patella vulgata) ≥85,000 units/mL ≤7,500 units/mL Helix pomatia Source G0751 Solid β-Glucuronidase from Helix pomatia Type H-1 ≥300,000 units/g solid ≥10,000 units/g solid G1512 Solid β-Glucuronidase from Helix pomatia Type H-5 ≥400,000 units/g solid ≤40,000 units/g solid G7017 Liquid β-Glucuronidase from Helix pomatia Type HP-2 ≥100,000 units/mL ≤7,500 units/mL G0876 Liquid β-Glucuronidase from Helix pomatia Type H-2 ≥85,000 units/mL ≤7,500 units/mL G7770 Liquid β-Glucuronidase from Helix pomatia Type HP-2S 90,000 units/mL ≤7,500 units/mL G8885 Liquid β-Glucuronidase from Helix pomatia Type H-3 ≥90,000 units/mL ≤1,000 units/mL G0762 Liquid β-Glucuronidase from Helix pomatia Type H-3AF ≥60,000 units/mL ≤1,000 unit/mL β-Glucuronidase from E. coli Source Cat. No. Form Product Description Glucuronidase Activity Sulfatase Activity G7396 Solid β-Glucuronidase from Escherichia coli Type IX-A 1,000,000–5,000,000 units/g protein Little to no activity G7646 Solid β-Glucuronidase from Escherichia coli Type VII-A 5,000,000–20,000,000 units/g protein Little to no activity G8295 Solid β-Glucuronidase from Escherichia coli Recombinant ≥10,000,000 units/g protein Little to no activity G8420 Solid β-Glucuronidase from Escherichia coli Recombinant ≥20,000 units/mg protein Little to no activity G8162 Liquid β-Glucuronidase from Escherichia coli ≥5,000,000 units/g protein Little to no activity

DISTRIBUTED BY:

10700 Rockley Road • Houston, Texas 77099 Phone: 281-496-0900 • Fax: 281-496-0400

[email protected] • www.expotechusa.com