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Table I: Incubation of Primary Human Endothelial Cells with IFN-Γ Results in a Significant Increase in the Transcription of 215 Genes

Table I: Incubation of Primary Human Endothelial Cells with IFN-Γ Results in a Significant Increase in the Transcription of 215 Genes

Table I: Incubation of primary human endothelial cells with IFN-γ results in a significant increase in the transcription of 215

Locus Fold ID Accession # Name Symbol Changeb p value 6373 AF002985 chemokine (C-X-C motif) ligand 11 CXCL11 298.28 5.66E-06 6373 AF030514 chemokine (C-X-C motif) ligand 11 CXCL11 229.77 2.59E-08 3627 NM_001565 chemokine (C-X-C motif) ligand 10 CXCL10 178.82 1.86E-08 3620 M34455 indoleamine-pyrrole 2,3 dioxygenase INDO 117.81 3.77E-06 4283 NM_002416 chemokine (C-X-C motif) ligand 9 CXCL9 78.17 8.36E-07 2633 AW014593 guanylate binding 1, interferon-inducible, 67kDa GBP1 52.15 1.07E-03 2633 NM_002053 guanylate binding protein 1, interferon-inducible, 67kDa GBP1 43.83 6.36E-04 2633 BC002666 guanylate binding protein 1, interferon-inducible, 67kDa GBP1 27.75 6.19E-04 115362 BG545653 Guanylate binding protein 5 GBP5 27.12 1.50E-05 NAc AI075407 NAc NAc 25.87 2.24E-03 94240 AA633203 epithelial stromal interaction 1 (breast) EPSTI1 23.43 2.87E-05 NAc AW392551 NAc NAc 22.64 3.81E-06 5920 NM_004585 retinoic acid receptor responder (tazarotene induced) 3 RARRES3 19.87 9.68E-07 2634 BF509371 guanylate binding protein 2, interferon-inducible GBP2 19.58 2.15E-03 10673 AF134715 (ligand) superfamily, member 13b TNFSF13B 19.38 1.18E-06 6890 NM_000593 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) TAP1 18.23 9.80E-07 6355 AI984980 chemokine (C-C motif) ligand 8 CCL8 17.67 5.16E-07 10537 NM_006398 gamma-aminobutyric acid (GABA) B receptor, 1 GABBR1 16.74 1.44E-05 51365 NM_015900 phospholipase A1 member A PLA1A 15.62 1.24E-04 115362 BG271923 Guanylate binding protein 5 GBP5 15.60 1.22E-04 10964 NM_006820 interferon-induced protein 44-like IFI44L 15.36 1.73E-03 3437 NM_001549 interferon-induced protein with tetratricopeptide repeats 3 IFIT3 14.98 2.03E-04 54625 AA056548 poly (ADP-) polymerase family, member 14 PARP14 14.29 1.66E-04 151636 AA577672 deltex 3-like (Drosophila) DTX3L 13.56 5.42E-03 NAc AW151360 NAc NAc 13.18 4.11E-04 51056 NM_015907 leucine aminopeptidase 3 LAP3 11.64 2.40E-03 6376 U84487 chemokine (C-X3-C motif) ligand 1 CX3CL1 11.22 4.14E-03 proteasome (prosome, macropain) subunit, beta type, 9 (large 5698 NM_002800 multifunctional peptidase 2) PSMB9 11.12 9.12E-04 6398 BF939675 secreted and transmembrane 1 SECTM1 10.64 2.88E-02 2643 NM_000161 GTP cyclohydrolase 1 (dopa-responsive dystonia) GCH1 10.56 1.02E-03 219285 BE966604 sterile alpha motif domain containing 9-like SAMD9L 10.20 6.84E-04 1462 BF218922 chondroitin sulfate proteoglycan 2 (versican) CSPG2 10.19 1.51E-05 8743 NM_003810 tumor necrosis factor (ligand) superfamily, member 10 TNFSF10 10.06 5.33E-03 2634 NM_004120 guanylate binding protein 2, interferon-inducible GBP2 10.05 1.39E-04 80833 NM_014349 apolipoprotein L, 3 APOL3 9.50 1.19E-03 116071 AW083820 basic leucine zipper transcription factor, ATF-like 2 BATF2 9.45 4.60E-04 Transcribed locus, strongly similar to XP_498081.1 PREDICTED: NAc AI694413 similar to Olfactory receptor 2I2 [Homo sapiens] NAc 9.25 1.10E-03 219285 BE669858 sterile alpha motif domain containing 9-like SAMD9L 9.17 7.46E-04 NAc W58601 Transcribed locus NAc 9.00 1.71E-05 3600 NM_000585 interleukin 15 IL15 8.83 2.97E-03 1193 AI768628 chloride intracellular channel 2 CLIC2 8.68 3.43E-03 115361 BG260886 guanylate binding protein 4 GBP4 8.66 7.64E-04 3659 NM_002198 interferon regulatory factor 1 IRF1 8.61 1.96E-04 Locus Fold ID Accession # Name Symbol Changeb p value NAc AI608902 NAc NAc 8.07 1.11E-04 2635 AL136680 guanylate binding protein 3 GBP3 8.02 6.20E-03 3431 NM_004509 SP110 nuclear body protein SP110 8.01 2.52E-04 57674 AA233374 17 open reading frame 27 C17orf27 7.75 1.19E-04 441168 AV734646 hypothetical protein LOC441168 RP1-93H18.5 7.66 3.68E-05 168537 AA858297 GTPase, IMAP family member 7 GIMAP7 7.59 1.64E-02 129607 AI742057 hypothetical protein LOC129607 LOC129607 7.51 8.40E-04 54739 AA142842 XIAP associated factor-1 BIRC4BP 7.46 2.97E-03 10068 AI521549 interleukin 18 binding protein IL18BP 7.39 3.52E-03 8743 AW474434 tumor necrosis factor (ligand) superfamily, member 10 TNFSF10 7.26 4.70E-02 64135 NM_022168 interferon induced with C domain 1 IFIH1 7.06 6.11E-03 6891 AA573502 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) TAP2 6.92 5.58E-03 1462 NM_004385 chondroitin sulfate proteoglycan 2 (versican) CSPG2 6.75 4.71E-04 6648 R34841 Superoxide dismutase 2, mitochondrial SOD2 6.74 2.75E-02 140711 BF437747 open reading frame 118 C20orf118 6.47 2.37E-04 5359 AI825926 phospholipid scramblase 1 PLSCR1 6.47 7.01E-03 10346 AA083478 tripartite motif-containing 22 TRIM22 6.38 2.78E-02 3431 AF280094 SP110 nuclear body protein SP110 6.37 1.26E-03 140711 AA603344 Chromosome 20 open reading frame 118 C20orf118 6.32 5.77E-04 80830 NM_030641 apolipoprotein L, 6 APOL6 6.25 4.45E-05 3433 AA131041 interferon-induced protein with tetratricopeptide repeats 2 IFIT2 6.19 9.80E-03 23586 NM_014314 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 DDX58 6.18 3.26E-04 7412 NM_001078 vascular cell adhesion molecule 1 VCAM1 6.16 3.06E-03 28984 NM_014059 response to complement 32 RGC32 6.05 4.17E-04 2920 M57731 chemokine (C-X-C motif) ligand 2 CXCL2 6.01 3.42E-03 6773 H98105 signal transducer and activator of transcription 2, 113kDa STAT2 6.00 2.08E-04 9111 NM_004688 N-myc (and STAT) interactor NMI 5.98 4.20E-03 54625 AW297731 Poly (ADP-ribose) polymerase family, member 14 PARP14 5.98 4.68E-02 6772 BC002704 signal transducer and activator of transcription 1, 91kDa STAT1 5.89 3.99E-03 84166 AA005023 nucleotide-binding oligomerization domains 27 NOD27 5.71 8.38E-05 6376 NM_002996 chemokine (C-X3-C motif) ligand 1 CX3CL1 5.57 3.66E-03 83666 AF307338 poly (ADP-ribose) polymerase family, member 9 PARP9 5.56 7.29E-03 8519 AA749101 interferon induced transmembrane protein 1 (9-27) IFITM1 5.56 4.64E-03 8542 AF323540 apolipoprotein L, 1 APOL1 5.49 1.57E-05 219285 AI064690 Sterile alpha motif domain containing 9-like SAMD9L 5.44 1.58E-04 23780 BC004395 apolipoprotein L, 2 APOL2 5.40 3.00E-04 716 M18767 complement component 1, s subcomponent C1S 5.37 1.53E-02 145741 BE218239 Nuclear localized factor 1 NLF1 5.36 3.54E-03 7453 M61715 tryptophanyl-tRNA synthetase WARS 5.36 5.40E-03 NAc AI655467 Full length insert cDNA clone YX74D05 NAc 5.21 1.68E-03 55601 NM_017631 hypothetical protein FLJ20035 FLJ20035 5.19 7.30E-03 5359 NM_021105 phospholipid scramblase 1 PLSCR1 5.14 4.34E-02 7453 NM_004184 tryptophanyl-tRNA synthetase WARS 5.05 4.20E-05 9246 NM_004223 ubiquitin-conjugating E2L 6 UBE2L6 4.89 1.08E-04 3628 NM_002194 inositol polyphosphate-1-phosphatase INPP1 4.85 4.56E-03 myxovirus (influenza virus) resistance 1, interferon-inducible 4599 NM_002462 protein p78 (mouse) MX1 4.79 2.84E-03 Locus Fold ID Accession # Name Symbol Changeb p value AFFX- HUMISGF3A/ 6772 M97935_3 signal transducer and activator of transcription 1, 91kDa STAT1 4.79 1.86E-02 3428 BG256677 interferon, gamma-inducible protein 16 IFI16 4.78 4.76E-02 94240 BE645480 Epithelial stromal interaction 1 (breast) EPSTI1 4.73 3.07E-03 91543 AI337069 radical S-adenosyl methionine domain containing 2 RSAD2 4.67 1.58E-03 similar to Caspase-4 precursor (CASP-4) (ICH-2 protease) (TX 648470 AA041298 protease) (ICE(rel)-II) LOC648470 4.59 1.56E-02 NAc AA781795 NAc NAc 4.57 5.06E-04 v-maf musculoaponeurotic fibrosarcoma oncogene homolog 4094 AF055376 (avian) MAF 4.56 4.74E-04 11217 BG540494 A kinase (PRKA) anchor protein 2 AKAP2 4.33 1.57E-02 NAc AW964431 NAc NAc 4.32 4.06E-04 91543 AW189843 radical S-adenosyl methionine domain containing 2 RSAD2 4.19 1.15E-03 9021 AI244908 suppressor of cytokine signaling 3 SOCS3 4.17 1.90E-02 8767 AF064824 receptor-interacting serine-threonine kinase 2 RIPK2 4.17 1.51E-03 3660 NM_002199 interferon regulatory factor 2 IRF2 4.15 3.13E-02 CD74 molecule, major histocompatibility complex, class II 972 K01144 invariant chain CD74 4.13 8.22E-04 3430 BC001356 interferon-induced protein 35 IFI35 4.08 4.83E-03 4939 NM_016817 2'-5'-oligoadenylate synthetase 2, 69/71kDa OAS2 4.07 3.39E-02 25939 AF147427 SAM domain and HD domain 1 SAMHD1 4.06 8.93E-03 7444 NM_006296 vaccinia related kinase 2 VRK2 4.04 8.94E-03 55859 NM_018476 brain expressed, X-linked 1 BEX1 3.99 1.78E-04 843 NM_001230 caspase 10, apoptosis-related cysteine peptidase CASP10 3.96 2.36E-04 3601 NM_002189 interleukin 15 receptor, alpha IL15RA 3.89 1.09E-03 64108 NM_022147 receptor transporter protein 4 RTP4 3.88 2.53E-04 1052 NM_005195 CCAAT/enhancer binding protein (C/EBP), delta CEBPD 3.85 4.59E-03 8519 NM_003641 interferon induced transmembrane protein 1 (9-27) IFITM1 3.82 2.15E-02 6737 NM_003141 tripartite motif-containing 21 TRIM21 3.82 2.09E-03 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, 834 M87507 convertase) CASP1 3.78 1.87E-02 121506 AI051248 chromosome 12 open reading frame 46 C12orf46 3.77 1.23E-03 10384 NM_006994 butyrophilin, subfamily 3, member A3 BTN3A3 3.76 1.63E-02 6772 NM_007315 signal transducer and activator of transcription 1, 91kDa STAT1 3.73 4.25E-03 3431 AF280095 SP110 nuclear body protein SP110 3.68 1.46E-02 54739 NM_017523 XIAP associated factor-1 BIRC4BP 3.67 1.11E-03 4938 NM_016816 2',5'-oligoadenylate synthetase 1, 40/46kDa OAS1 3.65 2.08E-02 55905 NM_018683 zinc finger protein 313 ZNF313 3.65 4.76E-03 10561 NM_006417 interferon-induced protein 44 IFI44 3.59 3.35E-02 961 BG230614 CD47 molecule CD47 3.58 1.88E-02 55281 NM_018295 transmembrane protein 140 TMEM140 3.55 2.69E-03 2355 N36408 FOS-like antigen 2 FOSL2 3.51 2.06E-02 55905 AF090934 zinc finger protein 313 ZNF313 3.50 1.67E-02 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, 834 U13698 convertase) CASP1 3.50 1.00E-02 10906 AB007447 TRAF-type zinc finger domain containing 1 TRAFD1 3.48 1.34E-03 10085 AA053711 EGF-like repeats and discoidin I-like domains 3 EDIL3 3.47 3.99E-02 NAc AU144114 NAc NAc 3.46 2.42E-03 Locus Fold ID Accession # Name Symbol Changeb p value NAc AV734646 NAc NAc 3.45 5.40E-04 197259 AA706818 mixed lineage kinase domain-like MLKL 3.44 7.37E-04 7127 NM_006291 tumor necrosis factor, alpha-induced protein 2 TNFAIP2 3.42 4.97E-03 1520 AK024855 cathepsin S CTSS 3.39 1.26E-03 961 Z25521 CD47 molecule CD47 3.37 4.98E-02 3434 NM_001548 interferon-induced protein with tetratricopeptide repeats 1 IFIT1 3.32 3.20E-02 8798 AF263541 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 DYRK4 3.32 2.41E-03 1462 BF590263 chondroitin sulfate proteoglycan 2 (versican) CSPG2 3.31 6.70E-04 54464 BG534738 5'-3' exoribonuclease 1 XRN1 3.30 3.30E-02 604 NM_001706 B-cell CLL/lymphoma 6 (zinc finger protein 51) BCL6 3.28 3.48E-03 54832 AA828371 Vacuolar protein sorting 13 homolog C (S. cerevisiae) VPS13C 3.27 3.12E-02 3428 AF208043 interferon, gamma-inducible protein 16 IFI16 3.26 4.13E-02 140691 BM976092 ring finger protein 36 RNF36 3.25 9.81E-03 10221 NM_025195 tribbles homolog 1 (Drosophila) TRIB1 3.24 2.51E-02 55905 AL031685 zinc finger protein 313 ZNF313 3.22 9.45E-03 solute carrier family 6 (neurotransmitter transporter, taurine), 6533 NM_003043 member 6 SLC6A6 3.22 3.22E-02 91351 AK023743 hypothetical protein FLJ31033 FLJ31033 3.22 5.29E-03 467 NM_001674 activating transcription factor 3 ATF3 3.22 5.61E-04 170575 NM_130759 GTPase, IMAP family member 1 GIMAP1 3.22 3.45E-02 5997 NM_002923 regulator of G-protein signalling 2, 24kDa RGS2 3.22 1.76E-03 10384 NM_006994 butyrophilin, subfamily 3, member A3 BTN3A3 3.22 1.46E-02 10384 U90548 butyrophilin, subfamily 3, member A3 BTN3A3 3.21 2.59E-02 25939 NM_015474 SAM domain and HD domain 1 SAMHD1 3.19 3.10E-03 4938 NM_002534 2',5'-oligoadenylate synthetase 1, 40/46kDa OAS1 3.19 1.63E-02 5366 NM_021127 phorbol-12-myristate-13-acetate-induced protein 1 PMAIP1 3.18 2.80E-03 56990 AF131831 CDC42 small effector 2 CDC42SE2 3.16 3.26E-02 leucine-rich repeats and calponin homology (CH) domain 57631 AK022128 containing 2 LRCH2 3.14 2.89E-02 51131 BF055474 PHD finger protein 11 PHF11 3.13 3.52E-02 56987 AF283775 bobby sox homolog (Drosophila) BBX 3.12 2.34E-02 127544 AL031602 IBR domain containing 3 IBRDC3 3.11 5.74E-03 BTB and CNC homology 1, basic leucine zipper transcription 571 NM_001186 factor 1 BACH1 3.08 2.69E-02 6672 NM_003113 SP100 nuclear antigen SP100 3.06 1.14E-03 NAc AI686890 Transcribed locus NAc 3.06 1.12E-02 56990 BE675995 CDC42 small effector 2 CDC42SE2 3.01 4.50E-02 283070 AA528080 hypothetical protein LOC283070 LOC283070 2.98 3.83E-03 22836 BE620739 Rho-related BTB domain containing 3 RHOBTB3 2.96 2.56E-02 84632 AW138743 KIAA1914 KIAA1914 2.95 2.00E-02 v-maf musculoaponeurotic fibrosarcoma oncogene homolog 4094 NM_005360 (avian) MAF 2.92 2.61E-03 10561 BE049439 Interferon-induced protein 44 IFI44 2.92 3.22E-03 388125 BE672687 Nuclear localized factor 2 NLF2 2.88 1.65E-03 10111 AI143416 RAD50 homolog (S. cerevisiae) RAD50 2.87 1.34E-03 5699 NM_002801 proteasome (prosome, macropain) subunit, beta type, 10 PSMB10 2.86 3.25E-02 55303 NM_018326 GTPase, IMAP family member 4 GIMAP4 2.84 2.17E-02 6772 AI539443 Signal transducer and activator of transcription 1, 91kDa STAT1 2.84 3.20E-03 10616 BE788439 chromosome 20 open reading frame 18 C20orf18 2.83 4.61E-03 Locus Fold ID Accession # Name Symbol Changeb p value 3717 AF001362 Janus kinase 2 (a protein tyrosine kinase) JAK2 2.80 3.79E-02 56951 NM_020199 open reading frame 15 C5orf15 2.79 5.40E-03 5464 NM_021129 (inorganic) 1 PPA1 2.79 2.63E-02 283131 AU155361 trophoblast-derived noncoding RNA TncRNA 2.77 1.48E-02 3075 X56210 complement factor H CFH 2.77 3.98E-02 5771 AI828880 protein tyrosine phosphatase, non-receptor type 2 PTPN2 2.76 6.37E-03 80830 BM980001 apolipoprotein L, 6 APOL6 2.67 1.53E-02 NAc T65020 CDNA FLJ32568 fis, clone SPLEN2000098 NAc 2.64 1.10E-03 carcinoembryonic antigen-related cell adhesion molecule 1 (biliary 634 X16354 ) CEACAM1 2.63 6.12E-03 1540 BE046443 cylindromatosis (turban tumor syndrome) CYLD 2.62 9.66E-03 1404 U43328 hyaluronan and proteoglycan link protein 1 HAPLN1 2.61 4.26E-02 51296 NM_016582 solute carrier family 15, member 3 SLC15A3 2.60 4.17E-02 55337 AI862559 hypothetical protein FLJ11286 FLJ11286 2.55 3.56E-02 81894 AF267854 solute carrier family 25, member 28 SLC25A28 2.55 9.69E-03 4261 NM_000246 class II, major histocompatibility complex, transactivator CIITA 2.54 4.82E-03 7424 U58111 vascular endothelial growth factor C VEGFC 2.54 4.66E-03 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, 834 AI719655 convertase) CASP1 2.53 4.87E-02 5721 NM_002818 proteasome (prosome, macropain) activator subunit 2 (PA28 beta) PSME2 2.53 5.80E-03 154761 AI928764 hypothetical protein LOC154761 LOC154761 2.52 1.09E-02 261726 NM_152902 TIP41, TOR signalling pathway regulator-like (S. cerevisiae) TIPRL 2.50 6.81E-03 355 AA164751 Fas (TNF receptor superfamily, member 6) FAS 2.50 1.06E-02 NAc BE889628 NAc NAc 2.48 2.57E-02 8507 NM_003633 ectodermal-neural cortex (with BTB-like domain) ENC1 2.48 1.90E-02 NAc AI733194 Transcribed locus NAc 2.48 1.16E-03 NAc AI761675 NAc NAc 2.46 6.91E-03 202 U83115 absent in melanoma 1 AIM1 2.45 2.77E-02 3301 AL534104 DnaJ (Hsp40) homolog, subfamily A, member 1 DNAJA1 2.45 4.83E-02 8837 AF005775 CASP8 and FADD-like apoptosis regulator CFLAR 2.45 8.68E-03 57665 AK023625 retinol dehydrogenase 14 (all-trans/9-cis/11-cis) RDH14 2.44 1.35E-02 5873 AF125393 RAB27A, member RAS oncogene family RAB27A 2.44 4.40E-02 25878 AF245505 matrix-remodelling associated 5 MXRA5 2.44 1.78E-02 10906 NM_006700 TRAF-type zinc finger domain containing 1 TRAFD1 2.43 1.13E-03 1404 NM_001884 hyaluronan and proteoglycan link protein 1 HAPLN1 2.42 3.59E-02 159091 AI629041 Hypothetical protein BC017868 RP3-473B4.1 2.41 3.66E-02 65983 AW575493 GRAM domain containing 3 GRAMD3 2.41 2.56E-02 51667 AF300717 NEDD8 ultimate buster-1 NYREN18 2.41 1.85E-02 7026 AV703465 nuclear receptor subfamily 2, group F, member 2 NR2F2 2.40 2.24E-02 3394 AI073984 interferon regulatory factor 8 IRF8 2.40 4.50E-03 51741 AK094336 WW domain containing WWOX 2.39 3.03E-02 6672 U36501 SP100 nuclear antigen SP100 2.39 2.68E-02 6347 S69738 chemokine (C-C motif) ligand 2 CCL2 2.36 2.34E-02 83666 AI738416 poly (ADP-ribose) polymerase family, member 9 PARP9 2.36 2.09E-03 8502 AA194254 plakophilin 4 PKP4 2.35 3.02E-02 8837 U97075 CASP8 and FADD-like apoptosis regulator CFLAR 2.34 1.19E-02 375035 AL035297 SFT2 domain containing 2 SFT2D2 2.34 1.74E-02 3371 NM_002160 tenascin C (hexabrachion) TNC 2.34 6.51E-03 Locus Fold ID Accession # Name Symbol Changeb p value 950 NM_005506 scavenger receptor class B, member 2 SCARB2 2.33 4.40E-02 10133 NM_021980 optineurin OPTN 2.33 2.75E-03 3965 NM_009587 lectin, galactoside-binding, soluble, 9 (galectin 9) LGALS9 2.32 4.74E-02 624 NM_000623 bradykinin receptor B2 BDKRB2 2.32 3.71E-02 5437 U37689 polymerase (RNA) II (DNA directed) polypeptide H POLR2H 2.32 2.21E-03 NAc BC013288 Homo sapiens, clone IMAGE:4042735, mRNA NAc 2.31 2.50E-03 837 U25804 caspase 4, apoptosis-related cysteine peptidase CASP4 2.28 3.41E-02 27445 AB011131 piccolo (presynaptic cytomatrix protein) PCLO 2.28 4.63E-02 9636 NM_005101 ISG15 ubiquitin-like modifier ISG15 2.27 1.94E-02 7098 NM_003265 toll-like receptor 3 TLR3 2.26 4.70E-02 84955 AI802955 NudC domain containing 1 NUDCD1 2.25 2.98E-02 NAc BF448315 ARTC1 mRNA, complete sequence NAc 2.25 8.48E-03 Full-length cDNA clone CS0DK002YF13 of HeLa cells Cot 25- NAc BG251692 normalized of Homo sapiens (human) NAc 2.25 3.24E-03 57047 NM_020359 phospholipid scramblase 2 PLSCR2 2.24 1.65E-02 56987 AI795970 bobby sox homolog (Drosophila) BBX 2.23 5.95E-03 4921 NM_006182 discoidin domain receptor family, member 2 DDR2 2.22 3.13E-02 4277 NM_005931 MHC class I polypeptide-related sequence B MICB 2.21 1.39E-02 NAc AL119957 NAc NAc 2.21 9.62E-03 5993 AW027312 regulatory factor X, 5 (influences HLA class II expression) RFX5 2.20 2.49E-02 222171 AI347918 hypothetical protein LOC222171 LOC222171 2.20 3.60E-02 284058 AI823453 KIAA1267 KIAA1267 2.19 2.98E-03 23194 BF692592 F-box and leucine-rich repeat protein 7 FBXL7 2.18 1.86E-02 24138 NM_012420 interferon-induced protein with tetratricopeptide repeats 5 IFIT5 2.18 1.36E-02 6734 BG474541 signal recognition particle receptor ('docking protein') SRPR 2.17 1.34E-02 3669 U88964 interferon stimulated exonuclease gene 20kDa ISG20 2.16 3.69E-03 2533 BF679849 FYN binding protein (FYB-120/130) FYB 2.15 3.12E-02 NAc AI799915 Clone DPDP-3 dental pulp-derived protein 3, mRNA sequence NAc 2.15 4.13E-02 29126 AF233516 CD274 molecule CD274 2.14 2.48E-02 NAc BG197463 CDNA clone IMAGE:5295612 NAc 2.14 1.19E-02 23549 AF005050 aspartyl aminopeptidase DNPEP 2.11 3.85E-02 28984 AI744499 Response gene to complement 32 RGC32 2.11 1.25E-02 10018 AI949179 BCL2-like 11 (apoptosis facilitator) BCL2L11 2.10 4.70E-02 9659 AA632758 Phosphodiesterase 4D interacting protein (myomegalin) PDE4DIP 2.10 4.45E-02 65987 AI672101 potassium channel tetramerisation domain containing 14 KCTD14 2.09 3.87E-03 57169 AA150460 zinc finger, NFX1-type containing 1 ZNFX1 2.08 9.69E-03 1021 AW274756 -dependent kinase 6 CDK6 2.07 3.70E-02 4299 AI768144 AF4/FMR2 family, member 1 AFF1 2.06 1.35E-02 NAc AI760356 MRNA; cDNA DKFZp586K1922 (from clone DKFZp586K1922) NAc 2.06 1.14E-02 6443 U29586 sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) SGCB 2.06 2.77E-02 643977 BE875232 hypothetical protein LOC643977 FLJ32255 2.05 3.94E-02 3109 NM_002118 major histocompatibility complex, class II, DM beta HLA-DMB 2.05 4.55E-03 6891 M74447 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) TAP2 2.04 1.20E-02 28996 BF594155 Homeodomain interacting 2 HIPK2 2.04 2.55E-02 23424 AW129593 tudor domain containing 7 TDRD7 2.04 2.64E-02 4008 AA100793 LIM domain 7 LMO7 2.03 2.47E-03 65987 NM_023930 potassium channel tetramerisation domain containing 14 KCTD14 2.03 2.43E-02 Locus Fold ID Accession # Name Symbol Changeb p value Transcribed locus, strongly similar to NP_689758.1 RasGEF NAc BF110534 domain family, member 1B; GPI-gamma 4 [Homo sapiens] NAc 2.02 4.17E-02 NAc AL137430 NAc NAc 2.02 2.62E-03 23360 AI083506 formin binding protein 4 FNBP4 2.02 1.98E-02 355 X83493 Fas (TNF receptor superfamily, member 6) FAS 2.01 3.24E-02 54464 AY137776 5'-3' exoribonuclease 1 XRN1 2.01 2.86E-02 10437 NM_006332 interferon, gamma-inducible protein 30 IFI30 2.01 5.72E-03

a Genes that have been classified according to the Consortium as mediators of

inflammation appear in bold and those which have been classified as participants of the immune

response appear italicized.

b Fold change in levels versus untreated HUVEC. Values shown are based on the

mean expression intensities obtained from two separate microarray experiments, normalized by

Robust Multichip Average (18).

c Not annotated Table II: Stimulation of HUVEC with IFN-γ results in a decrease in the transcription of 62 genes

Locus Fold ID Accession # Namea Symbol Changeb p value 7056 NM_000361 thrombomodulin THBD -4.43 1.42E-02 NAc BF439063 Transcribed locus NAc -4.16 2.34E-04 9518 AF003934 growth differentiation factor 15 GDF15 -4.12 1.27E-03 3357 NM_000867 5-hydroxytryptamine (serotonin) receptor 2B HTR2B -3.54 1.84E-02 84935 AW084730 hypothetical protein FLJ14834 FLJ14834 -3.21 1.41E-03 3624 M13436 inhibin, beta A (activin A, activin AB alpha polypeptide) INHBA -3.13 5.13E-03 6659 NM_003107 SRY (sex determining region Y)-box 4 SOX4 -3.06 9.21E-03 5569 NM_006823 protein kinase (cAMP-dependent, catalytic) inhibitor alpha PKIA -2.88 3.83E-02 7378 NM_003364 1 UPP1 -2.69 1.48E-02 tumor necrosis factor receptor superfamily, member 10d, decoy with 8793 AI738556 truncated death domain TNFRSF10D -2.62 7.21E-03 4953 NM_002539 ornithine decarboxylase 1 ODC1 -2.58 4.24E-02 79960 NM_024900 PHD finger protein 17 PHF17 -2.57 9.02E-03 8870 NM_003897 immediate early response 3 IER3 -2.57 2.26E-02 7851 BC003179 mal, T-cell differentiation protein-like MALL -2.56 4.00E-03 64129 NM_022164 tubulointerstitial nephritis antigen-like 1 TINAGL1 -2.55 4.69E-02 339123 AL577395 hypothetical LOC339123 LOC339123 -2.55 1.76E-02 5998 NM_021106 regulator of G-protein signalling 3 RGS3 -2.51 6.88E-03 79709 NM_024656 25 domain containing 1 GLT25D1 -2.49 4.09E-02 1660 NM_001357 DEAH (Asp-Glu-Ala-His) box polypeptide 9 DHX9 -2.49 1.75E-02 6723 NM_003132 spermidine synthase SRM -2.47 4.97E-02 3487 NM_001552 insulin-like growth factor binding protein 4 IGFBP4 -2.46 3.87E-02 fascin homolog 1, actin-bundling protein (Strongylocentrotus 6624 NM_003088 purpuratus) FSCN1 -2.41 3.49E-02 3339 M85289 heparan sulfate proteoglycan 2 (perlecan) HSPG2 -2.39 4.65E-02 23129 AL575403 plexin D1 PLXND1 -2.34 4.44E-02 64943 NM_022908 5'-nucleotidase domain containing 2 NT5DC2 -2.34 2.46E-02 646324 AI739132 Hypothetical protein LOC646324 LOC646324 -2.34 1.26E-03 NAc AA772172 NAc NAc -2.33 5.41E-03 57574 AB037820 membrane-associated ring finger (C3HC4) 4 NAc -2.33 2.94E-02 10939 NM_006796 AFG3 ATPase family gene 3-like 2 (yeast) AFG3L2 -2.32 4.57E-02 2006 AA479278 elastin (supravalvular aortic stenosis, Williams-Beuren syndrome) ELN -2.30 1.92E-02 56008 AU157605 hypothetical protein FLJ11236 FLJ11236 -2.25 4.17E-02 1984 BC000751 eukaryotic translation initiation factor 5A EIF5A -2.25 2.99E-02 10587 AB019695 thioredoxin reductase 2 TXNRD2 -2.21 2.93E-03 221749 AK024828 chromosome 6 open reading frame 145 C6orf145 -2.19 1.04E-02 7791 BC002323 zyxin ZYX -2.18 4.36E-02 8322 NM_012193 frizzled homolog 4 (Drosophila) FZD4 -2.18 2.38E-03 9466 AW295066 interleukin 27 receptor, alpha IL27RA -2.17 4.38E-02 80256 NM_025182 KIAA1539 KIAA1539 -2.17 1.97E-02 2316 NM_001456 filamin A, alpha (actin binding protein 280) FLNA -2.16 1.98E-02 283209 BE138757 phosphoglucomutase 2-like 1 PGM2L1 -2.16 1.88E-02 Locus Fold ID Accession # Namea Symbol Changeb p value 3678 NM_002205 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) ITGA5 -2.15 7.19E-03 5915 NM_000965 retinoic acid receptor, beta RARB -2.14 4.81E-02 microtubule associated monoxygenase, calponin and LIM domain 9645 BE965029 containing 2 MICAL2 -2.14 4.57E-02 56937 AL035541 transmembrane, prostate androgen induced RNA TMEPAI -2.13 3.15E-02 1839 NM_001945 heparin-binding EGF-like growth factor HBEGF -2.13 6.58E-03 1839 M60278 heparin-binding EGF-like growth factor HBEGF -2.13 4.26E-03 4066 BC002796 lymphoblastic leukemia derived sequence 1 LYL1 -2.13 2.85E-02 57661 BC004950 CTD-binding SR-like protein rA9 KIAA1542 -2.09 3.59E-02 79411 NM_024506 galactosidase, beta 1-like GLB1L -2.08 2.69E-02 1717 NM_001360 7-dehydrocholesterol reductase DHCR7 -2.08 1.96E-02 374907 AI760623 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 B3GNT8 -2.06 9.21E-03 84878 W63776 zinc finger protein 499 ZNF499 -2.06 2.56E-02 8605 AF065214 phospholipase A2, group IVC (cytosolic, calcium-independent) PLA2G4C -2.05 3.23E-02 sema domain, transmembrane domain (TM), and cytoplasmic 10501 AB022433 domain, (semaphorin) 6B SEMA6B -2.05 1.26E-02 53838 NM_022338 open reading frame 24 C11orf24 -2.05 3.99E-02 2030 NM_004955 solute carrier family 29 (nucleoside transporters), member 1 SLC29A1 -2.04 1.56E-02 9260 NM_005451 PDZ and LIM domain 7 (enigma) PDLIM7 -2.04 4.82E-02 2114 AL575509 v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) ETS2 -2.03 3.31E-02 8692 NM_003773 hyaluronoglucosaminidase 2 HYAL2 -2.03 3.59E-02 9136 NM_004704 RNA, U3 small nucleolar interacting protein 2 RNU3IP2 -2.02 2.65E-02 2050 NM_004444 EPH receptor B4 EPHB4 -2.01 3.95E-02 dual specificity phosphatase 3 (vaccinia virus phosphatase VH1- 1845 AL048503 related) DUSP3 -2.01 4.14E-02 23396 AB011161 phosphatidylinositol-4-phosphate 5-kinase, type I, gamma PIP5K1C -2.00 4.06E-02

a Genes that have been classified according to the Gene Ontology Consortium as mediators of

inflammation appear in bold and those which have been classified as participants in the immune

response are italicized.

b Fold change in gene expression levels versus untreated HUVEC. Values shown are based on the mean

expression intensities obtained from two separate microarray experiments, normalized by Robust

Multichip Average (18).

c Not annotated

Table III: B. burgdorferi and IFN-γ synergistically downregulate the expression of 237 genes

Locus Bb + Synergy ID Accession # Namea Symbol IFN-γb Bbb IFN-γb Scorec 81688 BE465032 Chromosome 6 open reading frame 62 C6orf62 1.19 -1.17 -10.74 -5.35 3326 BG612458 heat shock protein 90kDa alpha (cytosolic), class B member 1 HSP90AB1 -1.17 -1.36 -13.36 -5.28 7184 AI582238 heat shock protein 90kDa beta (Grp94), member 1 HSP90B1 -1.08 -1.01 -9.32 -4.45 procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4- 5034 AK075503 hydroxylase), beta polypeptide P4HB -2.22 -1.37 -15.08 -4.20 2202 AI740711 EGF-containing fibulin-like extracellular matrix protein 1 EFEMP1 -3.41 -1.05 -18.35 -4.11 2017 N45140 Cortactin CTTN -2.85 -1.31 -13.57 -3.26 10576 AL545982 chaperonin containing TCP1, subunit 2 (beta) CCT2 -1.65 -1.57 -9.94 -3.09 9749 AW880875 Phosphatase and actin regulator 2 PHACTR2 -1.08 -1.05 -6.16 -2.90 2023 U88968 enolase 1, (alpha) ENO1 -3.03 -1.20 -12.09 -2.86 81688 BG252842 chromosome 6 open reading frame 62 C6orf62 1.08 -1.30 -5.94 -2.67 375 AA580004 ADP-ribosylation factor 1 ARF1 -1.86 -1.41 -8.71 -2.67 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N- 2589 BC038440 acetylgalactosaminyltransferase 1 (GalNAc-T1) GALNT1 -1.35 -1.03 -6.22 -2.61 NAd AW084125 NAd NAd -1.49 -1.14 -6.86 -2.61 6659 AL136179 SRY (sex determining region Y)-box 4 SOX4 -1.81 1.60 -6.13 -2.52 3329 BE256479 heat shock 60kDa protein 1 (chaperonin) HSPD1 -2.00 -1.17 -7.88 -2.49 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 3688 NM_033669 includes MDF2, MSK12) ITGB1 -2.01 -1.23 -7.96 -2.46 57509 AI695017 mitochondrial tumor suppressor 1 MTUS1 -1.96 -1.01 -7.18 -2.42 800 NM_004342 caldesmon 1 CALD1 -1.08 1.44 -4.23 -2.39 AFFX- 60 HSAC07/X00351_5 actin, beta ACTB -4.58 -1.67 -14.78 -2.36 4781 AI186739 nuclear factor I/B NFIB -1.52 1.18 -5.51 -2.32 4781 T90642 Nuclear factor I/B NFIB 1.19 -1.02 -4.27 -2.29 NAd AI697657 Transcribed locus NAd -1.07 -1.20 -5.15 -2.28 283209 AA736452 phosphoglucomutase 2-like 1 PGM2L1 -1.24 1.45 -4.37 -2.26 119587 BF726934 carboxypeptidase X (M14 family), member 2 CPXM2 -1.36 -1.19 -5.53 -2.17 6303 BE326919 Spermidine/spermine N1-acetyltransferase SAT -2.74 -1.02 -8.06 -2.15 10434 AF077198 lysophospholipase I LYPLA1 1.08 1.18 -3.69 -2.08 1984 NM_001970 eukaryotic translation initiation factor 5A EIF5A -1.43 -1.14 -5.32 -2.07 Similar to SLIT-ROBO Rho GTPase-activating protein 2 (srGAP2) 647135 AI916641 (Formin-binding protein 2) LOC647135 1.63 1.45 -2.59 -1.99 6418 Z95126 SET translocation (myeloid leukemia-associated) SET 1.61 1.08 -3.07 -1.98 10203 U17473 calcitonin receptor-like CALCRL 1.44 -1.13 -3.58 -1.96 10492 AI472757 synaptotagmin binding, cytoplasmic RNA interacting protein SYNCRIP 1.15 1.16 -3.36 -1.95 25912 BC008306 chromosome 1 open reading frame 43 C1orf43 -1.87 -1.15 -5.85 -1.94 9749 NM_014721 phosphatase and actin regulator 2 PHACTR2 1.82 1.28 -2.58 -1.94 1654 BG492602 DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked DDX3X -1.41 1.26 -4.26 -1.93 3326 AI218219 heat shock protein 90kDa alpha (cytosolic), class B member 1 HSP90AB1 -2.37 1.13 -6.21 -1.91 8436 NM_004657 serum deprivation response (phosphatidylserine binding protein) SDPR -1.88 -1.30 -6.06 -1.91 3183 BE898861 heterogeneous nuclear ribonucleoprotein C (C1/C2) HNRPC -2.27 -1.03 -6.24 -1.89 4154 AI697540 Muscleblind-like (Drosophila) MBNL1 2.09 1.88 -1.91 -1.89 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 3688 NM_133376 includes MDF2, MSK12) ITGB1 -1.35 1.09 -4.29 -1.89 4256 AW512787 matrix Gla protein MGP 1.48 1.05 -3.07 -1.88 10492 BE672181 synaptotagmin binding, cytoplasmic RNA interacting protein SYNCRIP -1.41 1.01 -4.52 -1.88 64710 AL035851 nuclear casein kinase and cyclin-dependent kinase 1 NUCKS1 1.43 1.07 -3.07 -1.87 387 AF498970 ras homolog gene family, member A RHOA -1.90 -1.32 -6.00 -1.87 Locus Bb + Synergy ID Accession # Namea Symbol IFN-γb Bbb IFN-γb Scorec 55353 AF317417 lysosomal associated protein transmembrane 4 beta LAPTM4B -1.60 -1.35 -5.49 -1.86 51313 NM_016613 open reading frame 18 C4orf18 1.13 -1.45 -4.33 -1.85 splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate 6426 NM_006924 splicing factor) SFRS1 1.00 -1.52 -4.65 -1.85 7056 NM_000361 thrombomodulin THBD -4.43 1.13 -9.76 -1.83 8754 AF495383 ADAM metallopeptidase domain 9 (meltrin gamma) ADAM9 -1.35 -1.18 -4.63 -1.83 3320 AI962933 heat shock protein 90kDa alpha (cytosolic), class A member 1 HSP90AA1 -1.35 -1.04 -4.30 -1.80 10097 NM_005722 ARP2 actin-related protein 2 homolog (yeast) ACTR2 -1.09 1.04 -3.69 -1.79 NAd AI041522 Transcribed locus NAd -1.36 1.01 -4.18 -1.78 27250 NM_014456 programmed cell death 4 (neoplastic transformation inhibitor) PDCD4 -1.17 -1.14 -4.10 -1.78 406991 BF674052 microRNA 21 MIRN21 1.52 1.47 -2.37 -1.77 55378 NM_018507 hypothetical protein PRO1843 PRO1843 1.54 -1.19 -3.25 -1.77 115207 AA551075 potassium channel tetramerisation domain containing 12 KCTD12 -1.33 -1.08 -4.26 -1.77 55623 NM_017736 THUMP domain containing 1 THUMPD1 -1.08 1.38 -3.19 -1.76 3843 AU148466 binding protein 5 RANBP5 -1.32 1.18 -3.81 -1.76 10959 NM_006815 transmembrane emp24 domain trafficking protein 2 TMED2 -1.99 -1.26 -5.70 -1.75 653121 AW006067 zinc finger and BTB domain containing 8 ZBTB8 -1.27 1.31 -3.56 -1.75 54443 NM_018685 anillin, actin binding protein (scraps homolog, Drosophila) ANLN 1.08 -1.36 -3.97 -1.74 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H 5743 AY151286 synthase and cyclooxygenase) PTGS2 -2.10 1.54 -4.78 -1.74 8125 BE560202 Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A ANP32A -2.25 1.32 -5.20 -1.73 5861 NM_004161 RAB1A, member RAS oncogene family RAB1A 1.10 1.24 -2.95 -1.72 9652 BE566023 KIAA0372 KIAA0372 -1.02 1.15 -3.22 -1.70 54873 AI082747 palmdelphin PALMD -1.18 -1.66 -4.79 -1.69 8323 NM_003506 frizzled homolog 6 (Drosophila) FZD6 1.35 1.65 -2.26 -1.68 NAd R92925 Transcribed locus NAd 1.28 -1.04 -3.05 -1.67 81555 NM_030799 Yip1 domain family, member 5 YIPF5 -1.07 -1.10 -3.62 -1.67 8763 BF669455 CD164 molecule, sialomucin CD164 1.19 1.24 -2.75 -1.67 25937 AA081084 WW domain containing transcription regulator 1 WWTR1 -1.48 1.03 -4.08 -1.67 54407 NM_018573 solute carrier family 38, member 2 SLC38A2 -1.69 1.14 -4.28 -1.66 5962 AI057093 radixin RDX 1.25 -1.35 -3.56 -1.65 57182 BF196876 ankyrin repeat domain 50 ANKRD50 -1.65 1.39 -3.90 -1.65 140609 AL080111 NIMA (never in mitosis gene a)-related kinase 7 NEK7 -1.26 1.57 -3.13 -1.65 10129 NM_023037 furry homolog (Drosophila) FRY 1.13 -1.22 -3.47 -1.65 1968 NM_001415 eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa EIF2S3 -1.19 -1.53 -4.46 -1.64 5718 NM_002816 proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 PSMD12 -1.21 1.56 -3.04 -1.64 7184 AK025862 heat shock protein 90kDa beta (Grp94), member 1 HSP90B1 1.01 -1.11 -3.41 -1.63 succinate dehydrogenase complex, subunit C, integral membrane 6391 AF080579 protein, 15kDa SDHC -1.03 -1.35 -3.86 -1.63 55197 T79568 hypothetical protein FLJ10656 P15RS -1.13 -1.04 -3.52 -1.62 4154 BF512200 muscleblind-like (Drosophila) MBNL1 1.22 -2.05 -4.61 -1.61 3895 BF589024 kinectin 1 ( receptor) KTN1 1.74 1.60 -1.93 -1.61 54407 NM_018976 solute carrier family 38, member 2 SLC38A2 -1.88 1.21 -4.31 -1.59 55573 AV646549 CDV3 homolog (mouse) CDV3 -1.34 -1.43 -4.40 -1.59 633 NM_001711 biglycan BGN -1.25 -1.60 -4.52 -1.58 3426 BC020718 complement factor I CFI 1.11 -1.31 -3.47 -1.57 821 AI761759 calnexin CANX -1.29 1.23 -3.30 -1.57 9867 AA142966 praja 2, RING-H2 motif containing PJA2 -1.20 1.14 -3.26 -1.57 3357 NM_000867 5-hydroxytryptamine (serotonin) receptor 2B HTR2B -3.54 1.13 -6.94 -1.57 5552 J03223 proteoglycan 1, secretory granule PRG1 -1.00 -1.08 -3.25 -1.56 Locus Bb + Synergy ID Accession # Namea Symbol IFN-γb Bbb IFN-γb Scorec 54536 AK002113 exocyst complex component 6 EXOC6 -1.80 1.12 -4.17 -1.55 NAd W26593 MRNA from HIV associated non-Hodgkin's lymphoma (clone hl1-98) NAd -1.48 1.15 -3.65 -1.55 22915 NM_007351 multimerin 1 MMRN1 -1.25 1.43 -3.02 -1.55 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N- 2589 U41514 acetylgalactosaminyltransferase 1 (GalNAc-T1) GALNT1 -1.76 1.35 -3.87 -1.55 801 AI653730 calmodulin 1 (phosphorylase kinase, delta) CALM1 -1.39 -1.04 -3.76 -1.55 7163 BE974098 tumor protein D52 TPD52 -1.04 -1.07 -3.25 -1.55 9948 AF274954 WD repeat domain 1 WDR1 1.17 -1.56 -3.74 -1.55 Similar to SLIT-ROBO Rho GTPase-activating protein 2 (srGAP2) 653464 BF977829 (Formin-binding protein 2) LOC653464 -1.07 1.23 -2.91 -1.54 6783 NM_005420 sulfotransferase family 1E, estrogen-preferring, member 1 SULT1E1 -1.01 1.08 -2.98 -1.54 950 AA885297 scavenger receptor class B, member 2 SCARB2 1.54 -1.49 -3.30 -1.54 1452 AF119911 casein kinase 1, alpha 1 CSNK1A1 -1.15 -1.22 -3.65 -1.54 7026 AL554245 nuclear receptor subfamily 2, group F, member 2 NR2F2 1.50 1.21 -2.29 -1.54 6845 NM_005638 synaptobrevin-like 1 SYBL1 1.18 1.37 -2.42 -1.53 54542 AF255304 membrane associated DNA binding protein MNAB 1.77 1.40 -1.96 -1.53 5445 AF001602 paraoxonase 2 PON2 1.05 1.01 -2.97 -1.52 55854 AV716798 likely ortholog of mouse immediate early response, erythropoietin 4 LEREPO4 -1.38 1.19 -3.37 -1.52 6711 NM_003128 spectrin, beta, non-erythrocytic 1 SPTBN1 -1.47 -1.15 -3.98 -1.52 1601 NM_001343 disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) DAB2 -1.73 1.71 -3.50 -1.51 23075 AB014540 SWAP-70 protein SWAP70 -1.48 1.36 -3.35 -1.51 114882 AL049923 oxysterol binding protein-like 8 OSBPL8 1.48 1.49 -2.04 -1.51 8087 AI990766 fragile X mental retardation, autosomal homolog 1 FXR1 1.26 -1.15 -2.94 -1.51 51552 AA919115 RAB14, member RAS oncogene family RAB14 1.06 1.23 -2.65 -1.51 10093 AF019888 actin related protein 2/3 complex, subunit 4, 20kDa ARPC4 -1.78 -1.16 -4.42 -1.50 9341 NM_004781 vesicle-associated membrane protein 3 (cellubrevin) VAMP3 1.01 -1.56 -3.82 -1.50 10983 AA020986 Cyclin I CCNI -1.46 -1.23 -4.04 -1.50 120 NM_019903 adducin 3 (gamma) ADD3 1.06 1.38 -2.50 -1.50 10055 AF110956 SUMO-1 activating enzyme subunit 1 SAE1 -2.03 -1.37 -5.08 -1.49 10159 NM_005765 ATPase, H+ transporting, lysosomal accessory protein 2 ATP6AP2 1.01 1.08 -2.84 -1.49 64853 NM_022831 chromosome 1 open reading frame 80 C1orf80 1.25 -1.00 -2.67 -1.49 79772 NM_024717 multiple C2 domains, transmembrane 1 MCTP1 1.29 1.60 -2.07 -1.48 157638 AL039862 family with sequence similarity 84, member B FAM84B -1.13 1.65 -2.57 -1.48 5529 BF529715 2, regulatory subunit B (B56), epsilon isoform PPP2R5E 1.47 1.46 -2.02 -1.48 5928 X71810 retinoblastoma binding protein 4 RBBP4 -1.01 -1.06 -3.06 -1.47 9819 NM_014779 TSC22 domain family, member 2 TSC22D2 1.11 1.70 -2.20 -1.47 8801 BF593940 succinate-CoA , GDP-forming, beta subunit SUCLG2 1.27 1.34 -2.26 -1.47 27230 AL136807 stress-associated endoplasmic reticulum protein 1 SERP1 -1.16 1.59 -2.63 -1.47 55660 Z78308 PRP40 pre-mRNA processing factor 40 homolog A (yeast) PRPF40A 1.17 1.32 -2.36 -1.46 51705 NM_016242 endomucin EMCN -1.46 1.05 -3.51 -1.46 8615 BE875592 vesicle docking protein p115 VDP 1.33 -1.18 -2.81 -1.46 800 D90453 caldesmon 1 CALD1 -1.14 1.22 -2.85 -1.45 3084 NM_004495 neuregulin 1 NRG1 1.91 1.32 -1.86 -1.45 8436 BF982174 serum deprivation response (phosphatidylserine binding protein) SDPR -1.02 1.01 -2.91 -1.45 Transcribed locus, strongly similar to NP_055107.3 chromobox NAd N54506 homolog 6 [Homo sapiens] NAd 1.21 -1.12 -2.82 -1.45 9337 AF180476 CCR4-NOT transcription complex, subunit 8 CNOT8 -1.53 1.05 -3.59 -1.45 50810 AL133102 hepatoma-derived growth factor, related protein 3 HDGFRP3 -1.31 1.13 -3.17 -1.44 NAd BE645241 NAd NAd 3.05 1.49 -1.44 -1.44 9782 AA577695 matrin 3 MATR3 1.07 1.20 -2.56 -1.44 Locus Bb + Synergy ID Accession # Namea Symbol IFN-γb Bbb IFN-γb Scorec 1848 BC003143 dual specificity phosphatase 6 DUSP6 -1.21 1.69 -2.60 -1.44 57211 AL033377 -coupled receptor 126 GPR126 -1.13 1.27 -2.76 -1.44 protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 5781 AF119855 1) PTPN11 -1.18 -1.27 -3.54 -1.44 10402 AB022918 ST3 beta-galactoside alpha-2,3-sialyltransferase 6 ST3GAL6 -1.96 1.55 -3.75 -1.44 51495 AJ271091 protein tyrosine phosphatase-like A domain containing 1 PTPLAD1 1.21 1.28 -2.31 -1.44 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation 7531 U28936 protein, epsilon polypeptide YWHAE -1.41 -1.61 -4.34 -1.43 10299 NM_005885 membrane-associated ring finger (C3HC4) 6 NAd 1.41 1.23 -2.18 -1.43 3417 BC012846 isocitrate dehydrogenase 1 (NADP+), soluble IDH1 1.21 1.29 -2.29 -1.43 NAd AI635774 MRNA; cDNA DKFZp586N0121 (from clone DKFZp586N0121) NAd -1.41 1.39 -3.04 -1.42 23012 AW779556 serine/threonine kinase 38 like STK38L 1.21 1.53 -2.11 -1.42 23014 AK001699 F-box protein 21 FBXO21 1.20 -1.03 -2.64 -1.42 23405 BF590131 Dicer1, Dcr-1 homolog (Drosophila) DICER1 1.74 2.10 -1.49 -1.42 283209 AV724329 phosphoglucomutase 2-like 1 PGM2L1 -2.35 1.42 -4.34 -1.42 55858 AW665624 TPA regulated locus TPARL 1.21 1.19 -2.36 -1.42 23394 NM_015339 activity-dependent neuroprotector ADNP -1.32 1.36 -2.91 -1.41 9653 AW151887 heparan sulfate 2-O-sulfotransferase 1 HS2ST1 1.31 1.17 -2.29 -1.41 51495 AI984229 protein tyrosine phosphatase-like A domain containing 1 PTPLAD1 1.17 1.16 -2.42 -1.41 DKFZP686A01 22998 AB029025 hypothetical protein 247 -1.23 1.26 -2.85 -1.41 3205 U41813 homeobox A9 HOXA9 1.01 1.30 -2.49 -1.41 3799 BF223224 kinesin family member 5B KIF5B -1.05 1.32 -2.55 -1.41 Transcribed locus, strongly similar to NP_002194.1 integrin alpha 2 precursor; Integrin, alpha-2 (CD49B; alpha-2 subunit of VLA-2 NAd N95414 receptor; platelet antigen Br) [Homo sapiens] NAd -1.99 1.93 -3.52 -1.41 NAd N92500 Transcribed locus NAd -1.01 -1.16 -3.06 -1.41 165215 AW043602 KIAA1946 KIAA1946 1.21 1.20 -2.33 -1.40 4163 BE967311 mutated in colorectal cancers MCC -1.84 1.28 -3.67 -1.40 9782 NM_018834 matrin 3 MATR3 -1.00 1.29 -2.48 -1.40 NAd H09085 CDNA clone IMAGE:4513453 NAd -1.33 -1.13 -3.43 -1.39 1284 AA909035 collagen, type IV, alpha 2 COL4A2 -1.02 -1.23 -3.13 -1.39 23022 AU157932 palladin, cytoskeletal associated protein PALLD -1.15 1.03 -2.95 -1.39 10424 BE879873 progesterone receptor membrane component 2 PGRMC2 -1.35 1.17 -3.07 -1.39 149013 NM_015383 neuroblastoma breakpoint family, member 14 NBPF14 -1.32 1.12 -3.07 -1.39 10810 AB020707 WAS , member 3 WASF3 -1.17 1.38 -2.63 -1.39 182 U61276 jagged 1 (Alagille syndrome) JAG1 1.52 1.13 -2.13 -1.38 144165 AA404269 prickle-like 1 (Drosophila) PRICKLE1 -1.17 -1.06 -3.09 -1.38 8773 NM_003825 synaptosomal-associated protein, 23kDa SNAP23 -1.37 -1.37 -3.77 -1.38 aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl 60496 AF302110 AASDHPPT 1.20 1.87 -1.89 -1.38 4893 NM_002524 neuroblastoma RAS viral (v-ras) oncogene homolog NRAS 1.66 1.27 -1.92 -1.38 4131 AA554833 microtubule-associated protein 1B MAP1B 1.06 1.70 -2.11 -1.38 1977 AW268640 eukaryotic translation initiation factor 4E EIF4E 1.19 1.05 -2.47 -1.38 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation 7534 U28964 protein, zeta polypeptide YWHAZ -1.41 -1.14 -3.51 -1.38 7416 AJ002428 voltage-dependent anion channel 1 VDAC1 -1.63 -1.08 -3.72 -1.37 23471 NM_014294 translocation associated membrane protein 1 TRAM1 -1.12 1.04 -2.86 -1.37 64848 AL049305 YTH domain containing 2 YTHDC2 1.04 1.56 -2.19 -1.37 813 AF257659 calumenin CALU -1.12 -1.39 -3.42 -1.37 9236 AK022459 progression 1 CCPG1 1.35 1.24 -2.11 -1.36 3320 BG420237 heat shock protein 90kDa alpha (cytosolic), class A member 1 HSP90AA1 1.33 1.78 -1.79 -1.36 Locus Bb + Synergy ID Accession # Namea Symbol IFN-γb Bbb IFN-γb Scorec 6418 D45198 SET translocation (myeloid leukemia-associated) SET -1.44 1.42 -2.93 -1.36 57534 AW243097 mindbomb homolog 1 (Drosophila) MIB1 -1.10 1.07 -2.77 -1.36 Platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 5048 AA502643 45kDa PAFAH1B1 1.25 1.10 -2.32 -1.36 54443 AK023208 anillin, actin binding protein (scraps homolog, Drosophila) ANLN 1.10 1.24 -2.34 -1.36 NAd AW188464 Full length insert cDNA clone YI48C03 NAd 1.05 1.36 -2.29 -1.36 5434 AI554759 polymerase (RNA) II (DNA directed) polypeptide E, 25kDa POLR2E -1.58 -1.08 -3.61 -1.36 9341 BC005941 vesicle-associated membrane protein 3 (cellubrevin) VAMP3 1.10 1.06 -2.52 -1.36 11228 BG484552 Ras association (RalGDS/AF-6) domain family 8 RASSF8 -1.57 1.49 -3.04 -1.35 3840 AI935246 karyopherin alpha 4 (importin alpha 3) KPNA4 1.12 1.39 -2.17 -1.35 23731 AA495988 chromosome 9 open reading frame 5 C9orf5 -1.53 1.11 -3.28 -1.35 54751 AF459643 filamin binding LIM protein 1 FBLIM1 -1.30 -1.09 -3.22 -1.35 9749 AA551142 phosphatase and actin regulator 2 PHACTR2 1.83 1.27 -1.80 -1.35 2665 D13988 GDP dissociation inhibitor 2 GDI2 -1.16 1.06 -2.84 -1.35 26225 AW291264 ADP-ribosylation factor-like 5A ARL5A 1.23 1.40 -2.06 -1.35 1488 N23018 C-terminal binding protein 2 CTBP2 -1.12 1.40 -2.46 -1.34 830 BC005338 capping protein (actin filament) muscle Z-line, alpha 2 CAPZA2 1.12 1.45 -2.11 -1.34 10092 AF017807 actin related protein 2/3 complex, subunit 5, 16kDa ARPC5 -1.11 -1.62 -3.63 -1.33 284119 AL545542 polymerase I and transcript release factor PTRF -3.28 -1.25 -6.03 -1.33 627 BF674612 Brain-derived neurotrophic factor BDNF -1.75 1.08 -3.57 -1.33 57381 BE218803 ras homolog gene family, member J RHOJ -1.77 -1.01 -3.69 -1.33 7171 BC002827 tropomyosin 4 TPM4 -1.97 -1.10 -4.08 -1.33 protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue 5573 NM_002734 specific extinguisher 1) PRKAR1A -1.11 1.32 -2.48 -1.33 30061 AL136944 solute carrier family 40 (iron-regulated transporter), member 1 SLC40A1 -1.01 1.49 -2.22 -1.32 7153 AL561834 topoisomerase (DNA) II alpha 170kDa TOP2A -1.12 1.18 -2.61 -1.32 57092 BC013916 PEST proteolytic signal containing nuclear protein PCNP 1.04 1.10 -2.47 -1.32 8895 AA541758 copine III CPNE3 1.24 -1.45 -2.97 -1.32 10006 BF673013 abl-interactor 1 ABI1 1.15 1.16 -2.28 -1.32 3146 BE311760 high-mobility group box 1 HMGB1 1.02 -1.23 -2.90 -1.32 9823 NM_014782 armadillo repeat containing, X-linked 2 ARMCX2 -1.52 1.16 -3.13 -1.31 1843 NM_004417 dual specificity phosphatase 1 DUSP1 -1.60 1.29 -3.11 -1.31 mediator of RNA polymerase II transcription, subunit 6 homolog (S. 10001 NM_005466 cerevisiae) MED6 1.04 -1.36 -3.03 -1.31 11059 BF131791 WW domain containing E3 ubiquitin protein ligase 1 WWP1 -1.10 1.44 -2.35 -1.30 4154 N31913 muscleblind-like (Drosophila) MBNL1 -1.70 1.09 -3.42 -1.30 11051 AL520677 nudix (nucleoside diphosphate linked moiety X)-type motif 21 NUDT21 1.03 1.33 -2.25 -1.30 10276 NM_005863 neuroepithelial cell transforming gene 1 NET1 -1.03 1.22 -2.40 -1.30 54796 AI377043 Basonuclin 2 BNC2 -1.09 1.20 -2.50 -1.30 9821 BG402105 RB1-inducible coiled-coil 1 RB1CC1 1.30 1.28 -2.02 -1.30 51454 AF200715 GULP, engulfment adaptor PTB domain containing 1 GULP1 -1.11 1.02 -2.72 -1.30 29966 NM_014574 striatin, calmodulin binding protein 3 STRN3 1.45 1.16 -2.02 -1.30 8754 NM_003816 ADAM metallopeptidase domain 9 (meltrin gamma) ADAM9 1.39 1.89 -1.62 -1.30 54665 AK022166 round spermatid basic protein 1 RSBN1 1.83 1.35 -1.67 -1.30 133619 AL524045 hypothetical protein MGC12103 LOC133619 -1.57 1.65 -2.82 -1.30 2956 D89646 mutS homolog 6 (E. coli) MSH6 -1.20 -1.37 -3.33 -1.29 205428 BE348597 chromosome 3 open reading frame 58 C3orf58 1.11 -1.04 -2.52 -1.29 55031 AF059318 ubiquitin specific peptidase 47 USP47 1.16 -1.21 -2.68 -1.29 25932 AF109196 chloride intracellular channel 4 CLIC4 -1.01 -1.31 -2.99 -1.29 81555 AW001618 Yip1 domain family, member 5 YIPF5 -1.42 1.62 -2.62 -1.29 Locus Bb + Synergy ID Accession # Namea Symbol IFN-γb Bbb IFN-γb Scorec 8560 NM_003676 degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) DEGS1 1.10 -1.06 -2.53 -1.29 10611 BG054550 PDZ and LIM domain 5 PDLIM5 1.10 -1.38 -2.95 -1.29 102 AU135154 ADAM metallopeptidase domain 10 ADAM10 -1.18 -1.32 -3.21 -1.29 5500 AI700962 Protein phosphatase 1, catalytic subunit, beta isoform PPP1CB 1.27 1.48 -1.88 -1.29 81555 AA169752 Yip1 domain family, member 5 YIPF5 -1.22 1.40 -2.49 -1.28 29005 AA827878 PRO1073 protein PRO1073 1.29 1.34 -1.96 -1.28 3397 D13889 inhibitor of DNA binding 1, dominant negative helix-loop-helix protein ID1 -2.33 -1.06 -4.36 -1.28 23741 AF109873 CREBBP/EP300 inhibitor 1 CRI1 1.17 1.23 -2.15 -1.28 10724 AF307332 meningioma expressed antigen 5 (hyaluronidase) MGEA5 1.25 1.30 -2.01 -1.28 7764 NM_006526 zinc finger protein 217 ZNF217 1.78 1.22 -1.78 -1.28 23136 BC006141 erythrocyte membrane protein band 4.1-like 3 EPB41L3 -1.09 1.35 -2.34 -1.28 80218 AU149868 Mak3 homolog (S. cerevisiae) MAK3 1.01 1.13 -2.40 -1.28 11010 AI912583 GLI pathogenesis-related 1 (glioma) GLIPR1 1.84 1.38 -1.62 -1.28 6009 AF493921 Ras homolog enriched in brain RHEB 1.16 1.32 -2.07 -1.28 4644 AW235548 VA (heavy polypeptide 12, myoxin) MYO5A -1.24 1.48 -2.45 -1.28 80204 AL117620 F-box protein 11 FBXO11 1.38 1.40 -1.84 -1.28 4089 NM_005359 SMAD, mothers against DPP homolog 4 (Drosophila) SMAD4 1.06 -1.25 -2.80 -1.28 10048 NM_005493 RAN binding protein 9 RANBP9 -1.40 1.11 -2.93 -1.27 115207 AI718937 potassium channel tetramerisation domain containing 12 KCTD12 1.11 1.63 -1.92 -1.27 64399 AY009951 hedgehog interacting protein HHIP 1.06 1.74 -1.93 -1.27 55754 AK022883 transmembrane protein 30A TMEM30A 1.19 -1.22 -2.61 -1.27 2335 AI333596 Fibronectin 1 FN1 -1.42 -1.07 -3.16 -1.27 284119 AF312393 polymerase I and transcript release factor PTRF -1.30 1.39 -2.56 -1.27 9147 BC006001 serologically defined colon cancer antigen 1 SDCCAG1 1.56 1.47 -1.68 -1.27 23196 AK091785 chromosome 9 open reading frame 10 C9orf10 1.24 1.06 -2.22 -1.27 216 NM_000689 aldehyde dehydrogenase 1 family, member A1 ALDH1A1 -1.48 -1.02 -3.16 -1.27 7812 NM_007158 cold shock domain containing E1, RNA-binding CSDE1 1.20 1.07 -2.24 -1.27 84295 AK027039 PHD finger protein 6 PHF6 1.11 1.48 -1.99 -1.26 50810 AB029156 hepatoma-derived growth factor, related protein 3 HDGFRP3 -1.07 1.32 -2.30 -1.26 26118 AL110243 WD repeat and SOCS box-containing 1 WSB1 1.92 1.61 -1.44 -1.26 23136 NM_012307 erythrocyte membrane protein band 4.1-like 3 EPB41L3 -1.48 1.39 -2.77 -1.26 Transcribed locus, strongly similar to NP_005594.1 ATPase, Class I, NAd BG290908 type 8B, member 1; [Homo sapiens] NAd -1.30 1.45 -2.50 -1.26 414327 AW450329 HBV preS1-transactivated protein 4 PS1TP4 1.06 1.25 -2.20 -1.26 64397 AI493587 zinc finger protein 106 homolog (mouse) ZFP106 -1.15 1.12 -2.57 -1.26 NAd AW294869 CDNA clone IMAGE:5299642 NAd 1.38 1.63 -1.68 -1.26 10959 NM_006815 transmembrane emp24 domain trafficking protein 2 TMED2 -1.07 -1.15 -2.79 -1.26 5087 AL049381 Pre-B-cell leukemia transcription factor 1 PBX1 -1.50 1.41 -2.77 -1.25 8773 U55936 synaptosomal-associated protein, 23kDa SNAP23 -1.21 -1.61 -3.54 -1.25 3087 Z21533 homeobox, hematopoietically expressed HHEX -1.06 1.25 -2.34 -1.25

a Genes that have been classified according to the Gene Ontology Consortium as mediators of

inflammation appear in bold and those which have been classified as participants of the immune

response appear italicized. b Fold change in gene expression levels versus untreated HUVEC. Values shown are based on the mean expression intensities obtained from two separate microarray experiments, normalized by

Robust Multichip Average (18). c The synergy score is equal to the inverse of the gene expression levels induced by B. burgdorferi and

IFN-γ combined divided by the sum of the inverses of the gene expression levels attributed to the individual stimuli; this number is multiplied by -1 to give the negative synergy score a negative value for representative purposes. d Not annotated

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